TFOBO increases Bax/Bcl2 levels, caspase9, and caspase3/7 activity and decreases mitochondrial membrane potential. (bvsalud.org)
Ca 2+ added from the outer side of mitochondrial membrane produced dual effect on mPTP activity: activation at the onset of depolarization, but consequent promotion of mPTP closure. (ukrbiochemjournal.org)
Based on the experiments, it was concluded that mitochondrial energization was required for prolonged mPTP functioning in sub-conductance states, whereas membrane depolarization promoted the transition of mPTP to inactive state during calcium release from mitochondria. (ukrbiochemjournal.org)
It was shown that bioenergetic effects of MPTP opening under steady state conditions (increase in the oxygen consumption rate under substrate oxidation without ADP, decrease in respiratory control ratio as well as the effectiveness of ATP synthesis, P/O) are close to the functional alterations, which result from the increase of endogenous proton conductance of mitochondrial membrane. (ukrbiochemjournal.org)
ABC transporters are considered to be an ABC superfamily based on the similarities of the sequence and organization of their ATP-binding cassette (ABC) domains, even though the integral membrane proteins appear to have evolved independently several times, and thus comprise different protein families. (wikipedia.org)
Potassium channel2
Effective pharmacotherapy against oxidative injury: alternative utility of an ATP-sensitive potassium channel opener. (uchicago.edu)
K ATP channels, widely represented in metabolically active tissues, are hetero-octamers composed of four regulatory SUR subunits (SUR1, SUR2A, or SUR2B) and four ATP-sensitive pore-forming inwardly rectifying potassium channel (Kir6.x) subunits (Kir6.1 or Kir6.2) [ 20 ]. (biomedcentral.com)
ABC transporters utilize the energy of ATP binding and hydrolysis to transport various substrates across cellular membranes. (wikipedia.org)
Kir6.21
Specifically, current through recombinant wild-type SUR1/Kir6.2 channels expressed in COS7 cells was activated by NO, but channels formed only from truncated isoform Kir6.2 subunits without SUR1 subunits were insensitive to NO. Further, mutagenesis of SUR1 indicated that NO-induced K ATP channel activation involves interaction of NO with residues in the NBD1 of the SUR1 subunit. (biomedcentral.com)
Ratio1
Because their opening is determined by the cytosolic ADP/ATP ratio, K ATP channels act as metabolic sensors, linking cytosolic energetics with cellular functions in various tissues [ 21 , 22 ]. (biomedcentral.com)
Subunits1
The ATPase subunits utilize the energy of adenosine triphosphate (ATP) binding and hydrolysis to provide the energy needed for the translocation of substrates across membranes, either for uptake or for export of the substrate. (wikipedia.org)
Mechanism1
The capacity of NO to activate K ATP channels via this mechanism remains intact even after spinal nerve ligation, thus providing opportunities for selective pharmacological enhancement of K ATP current even after decrease of this current by painful-like nerve injury. (biomedcentral.com)
Activation6
Furthermore, activation of K ATP channels is suppressed in DRG neurons after painful-like nerve injury. (biomedcentral.com)
Cell-attached and cell-free recordings of K ATP currents in large DRG neurons from control rats (sham surgery, SS) revealed activation of K ATP channels by NO exogenously released by the NO donor SNAP, through decreased sensitivity to [ATP]i. (biomedcentral.com)
This NO-induced K ATP channel activation was not altered in ganglia from animals that demonstrated sustained hyperalgesia-type response to nociceptive stimulation following spinal nerve ligation. (biomedcentral.com)
However, baseline opening of K ATP channels and their activation induced by metabolic inhibition was suppressed by axotomy. (biomedcentral.com)
Failure to block the NO-mediated amplification of K ATP currents with specific inhibitors of sGC and PKG indicated that the classical sGC/cGMP/PKG signaling pathway was not involved in the activation by SNAP. (biomedcentral.com)
NO-induced activation of K ATP channels remained intact in cell-free patches, was reversed by DTT, a thiol-reducing agent, and prevented by NEM, a thiol-alkylating agent. (biomedcentral.com)
Control1
Therefore, we investigated NO modulation of K ATP channels in control and axotomized DRG neurons. (biomedcentral.com)
Potassium15
1. The effect of atp-dependent potassium uptake on mitochondrial functions under acute hypoxia. (nih.gov)
12. Combined modulation of the mitochondrial ATP-dependent potassium channel and the permeability transition pore causes prolongation of the biphasic calcium dynamics. (nih.gov)
18. [Effect of ATP-sensitive potassium channel modulators and intermittent hypoxia on mitochondrial respiration during stress]. (nih.gov)
Furthermore, the effect of 14,15-EEZE on the cardioprotective actions of the selective mitochondrial ATP-sensitive potassium channel opener diazoxide was investigated. (nih.gov)
Two innovative libraries (413 cyclosulfamides and 709 orthopyridine sulfamides) of potential new openers of ATPsensitive potassium channels of cell membranes have been created. (org.ua)
7. Ishizaka H., Kuo L. Acidosis-induced coronary arteriolar dilation is mediated by ATP-sensitive potassium channels in vascular smooth muscle. (org.ua)
9. Strutyns'kyi R.B., Kotsiuruba A.V., Neshcheret O.P., Shysh A.M., Rovenets' R.A., Moibenko O.O. Cardioprotective effects of activation of ATP-sensitive potassium channels in experiments in vivo: influence on blood biochemical parameters following ischemia-reperfusion of the myocardium. (org.ua)
Creation and preparation to apply the new do mestic cardioprotective drug - fluorine-containing activator of ATP-dependent potassium channel Flocalin. (org.ua)
Nielsen JJ, Kristensen M, Hellsten Y, Bangsbo J, Juel C. Localization and function of ATP-sensitive potassium channels in human skeletal muscle. (ukrbiochemjournal.org)
Adenosine triphosphate1
The ATPase subunits utilize the energy of adenosine triphosphate (ATP) binding and hydrolysis to provide the energy needed for the translocation of substrates across membranes, either for uptake or for export of the substrate. (wikipedia.org)
Pharmacological3
It is shown experimentally that, at least, ten new original compounds have properties of pharmacological openers of the channels. (org.ua)
10. Wakahara N., Katoh H., Yaguchi Y., Uehara A., Satoh H., Terada H., Fujise Y., Hayashi H. Difference in the cardioprotective mechanisms between Ischemic preconditioning and pharmacological preconditioning by diazoxide in rat hearts. (org.ua)
Interaction of mitoK ATP with physiological and pharmacological ligands is discussed as well. (ukrbiochemjournal.org)
Cell membranes1
17. The influence of heavy metal ions, spermine and sodium nitroprusside on ATP-hydrolases of cell membranes of rat colon smooth muscle. (nih.gov)
Activators1
The SUR subunits contain binding sites for activators and blockers and have metabolic sensor, which enables channel activation under conditions of metabolic stress. (ukrbiochemjournal.org)
Cardioprotective3
11. Strutyns'kyi R.B., Kotsiuruba A.V., Rovenets' R.A., Strutyns'ka N.A., Iagupols'kyi Iu.L., Sagach V.F., Moibenko O.O. Biochemical mechanisms of the cardioprotective effect of the K(ATP) channels opener flocalin (medicinal form) in ischemia-reperfusion of myocardium. (org.ua)
Sarcolemmal cardiac KATP channels as a target for the cardioprotective effects of the fluorine-containing pinacidil analogue flocalin. (org.ua)
13. Strutyns'kyi R.B., Rovenets' R.A., Moibenko O.O. Mechanisms of cardioprotective action domestic opener of the KATP-channels flocalin. (org.ua)
Regulation2
16. The regulation of mitochondrial respiration by opening of mKCa channels is age-dependent. (nih.gov)
Farzaneh T, Tinker A. Differences in the mechanism of metabolic regulation of ATP-sensitive K+ channels containing Kir6.1 and Kir6.2 subunits. (ukrbiochemjournal.org)
Ischemic1
Activation of these channels under ischemic conditions underlies their cytoprotective action, which results in prevention of Ca 2+ overload in cytosol. (ukrbiochemjournal.org)
Vasodilation1
6. Strutyns'kyi R.B. The vasodilation effects of flokalin, a fluorine-containing K(ATP) channel opener. (org.ua)
3. [The effect of ATP-dependent K(+)-channel opener on the functional state and the opening of cyclosporine-sensitive pore in rat liver mitochondria]. (nih.gov)
However, functional activity of these channels does not depend on absolute concentration of ATP but on the АТР/ADP ratio and presence of Mg 2+ . (ukrbiochemjournal.org)
ABC transporters utilize the energy of ATP binding and hydrolysis to transport various substrates across cellular membranes. (wikipedia.org)
ATP-sensitive K + transport through the mitochondrial- membrane is easily tested by different methods, which are briefly reviewed in this paper. (ukrbiochemjournal.org)
Results1
Vasodilatory intervention with dopamine, fenoldopam, calcium channel blockers, and theophylline has been investigated, but results are inconsistent [ 11 - 14 ]. (e-ceth.org)
Type1
Z31197374 and Z666664306 compounds have showed the affinity only to the К ATP channels of the mitochondrial type. (org.ua)
Stress1
9. [Effect of K-ATP channel opener-pinacidil on the liver mitochondria function in rats with different resistance to hypoxia during stress]. (nih.gov)
Influence2
5. Influence of ATP-dependent K(+)-channel opener on K(+)-cycle and oxygen consumption in rat liver mitochondria. (nih.gov)
8. [The influence of ATP-dependent K(+)-channel diazoxide opener on the opening of mitochondrial permeability transition pore in rat liver mitochondria]. (nih.gov)