GroELHSPD1Hsp10Known as Rubisco-bindingEncoded mitochondrial proteinMitochondriaProteinsChaperonesPrecursorHSP65MammalianHomologousFragmentationInducesEndogenousOverexpressionHeatFunctionalProtein importKnockdownDysfunctionSurvivalPathwayExperimentSequenceInductionFamilySpeciesExpressionInteractionFunctionBioinformaticsTranscriptHumanIncreasesOrganelleComplexRolePromoterPlant
GroEL9
- The homolog in E. coli is GroES that is a chaperonin which usually works in conjunction with GroEL. (wikipedia.org)
- GroES exists as a ring-shaped oligomer of between six and eight identical subunits, while the 60 kDa chaperonin (cpn60, or groEL in bacteria) forms a structure comprising 2 stacked rings, each ring containing 7 identical subunits. (wikipedia.org)
- HSP60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. (abeomics.com)
- This study has demonstrated that expression of HSP60-HSP10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES. (abeomics.com)
- We serendipitously found that chaperonin GroEL can hydrolyze ortho-nitrophenyl ß-galactoside (ONPG), a well-known substrate of the enzyme ß-galactosidase. (bvsalud.org)
- The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. (thermofisher.cn)
- The TCP-1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL. (thermofisher.cn)
- The human Hsp60 is a member of a highly conserved family which includes molecular chaperones from several species including plant Hsp60 (known as Rubisco binding protein), and bacterial GroEL, a major antigen of mycobacteria. (enzolifesciences.com)
- Amino-acid sequence homology of a polymorphic cellular protein from human lymphocytes and the chaperonins from Escherichia coli (groEL) and chloroplasts (Rubisco-binding protein). (wikidata.org)
HSPD12
- We show heat shock protein family D member 1 (HSPD1 or HSP60) as a survival gene ubiquitously expressed in NSCLC and associated with poor patients' prognosis. (biomedcentral.com)
- These results highlight mitochondrial metabolism as an attractive target and HSPD1 as a potential theranostic marker for developing therapies to combat NSCLC. (biomedcentral.com)
Hsp106
- Heat shock 10 kDa protein 1 (Hsp10), also known as chaperonin 10 (cpn10) or early-pregnancy factor (EPF), is a protein that in humans is encoded by the HSPE1 gene. (wikipedia.org)
- These similarities are supported by recent studies where the single-ring human mitochondrial homolog, HSP60 with its co-chaperonin, HSP10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. (abeomics.com)
- Another important function of HSP60 and HSP10 is their protective functions against infection and cellular stress. (abeomics.com)
- Quantitative patterns of Hsps in tubular adenoma compared with normal and tumor tissues reveal the value of Hsp10 and Hsp60 in early diagnosis of large bowel cancer. (gzsys.org.cn)
- Consistent with their function as chaperones, Hsp60 and Hsp10 have been suggested to act as docking molecules with a passive role in the maturation of caspase processing. (enzolifesciences.com)
- Recombinant Hsp60 and Hsp10 have been shown to accelerate the activation of procaspase-3 by cytochrome c and dATP in an ATP-dependent manner. (enzolifesciences.com)
Known as Rubisco-binding1
- Whereas mammalian HSP60 is localized within the mitochondria, plant HSP60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. (abeomics.com)
Encoded mitochondrial protein1
- The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. (thermofisher.com)
Mitochondria3
- In line with this notion, mitochondria are highly dynamic organelles that undergo fission and fusion and move into the cell along the microtubules to generate the mitochondrial network [ 6 ]. (hindawi.com)
- Although studies suggest that αSyn and its missense mutant, A53T, preferentially accumulate in the mitochondria, the mechanisms by which αSyn and mitochondrial proteins regulate each other to trigger mitochondrial and neuronal toxicity are poorly understood. (springer.com)
- Despite these findings suggesting that mitochondria might be a key link between αSyn toxicity and neuronal degeneration in PD, the field still lacks an understanding of how αSyn abnormality and mitochondrial functional deficiency influence each other. (springer.com)
Proteins8
- Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. (abeomics.com)
- It has been indicated that these proteins carry out a very important biological function due to the fact that HSP60 is present in so many different species. (abeomics.com)
- Deficiency in ClpP induces an overload of mitochondrial misfolded/unfolded proteins, suppresses mitochondrial respiratory activity, increases mitochondrial oxidative damage and causes cell death. (springer.com)
- cis-acting sequences and trans-acting factors in the localization of mRNA for mitochondrial ribosomal proteins. (gzsys.org.cn)
- The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP70) including high abundance, induction by environmental stress, and ATPase activity. (thermofisher.cn)
- Hsps are intracellular proteins which are thought to serve protective functions against infection and cellular stress, however several studies indicate that members of the Hsp60 family are linked to a number of autoimmune diseases, artherosclerosis and chlamydial disease. (enzolifesciences.com)
- It provides a very informative, albeit preliminary, glimpse into the expression of genes encoding functionally relevant proteins as potential targets for chemotherapy, and evidence for the presence of a relict plastid. (biomedcentral.com)
- Integrated epigenetics of human breast cancer : synoptic investigation of targeted genes, microRNAs and proteins upon demethylation treatment. (unibas.ch)
Chaperones1
- An interconnected highly integrated system of mitochondrial and cytosolic chaperones and proteases along with the fission/fusion machinery represents the surveillance scaffold of mitostasis. (hindawi.com)
Precursor1
- Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides. (yeastrc.org)
HSP651
- Antibodies against Hsp60 and Hsp65 in the sera of women with ovarian cancer. (gzsys.org.cn)
Mammalian3
- It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian HSP60. (abeomics.com)
- Structural studies have provided valuable insights into the mechanism of this process, but real-time dynamics analyses of mammalian type II chaperonins are still scarce. (bvsalud.org)
- This study is the first to use DXT to probe the real-time molecular dynamics of mammalian type II chaperonins at the millisecond level. (bvsalud.org)
Homologous1
- Click gene/probe ID to show a list of genes that are homologous to the gene. (or.jp)
Fragmentation1
- Mitochondrial protein with a potential role in promoting mitochondrial fragmentation during programm. (yeastrc.org)
Induces2
- HSP60 induces the phosphorylation and nuclear localization of NF-κB both in vitro and in vivo. (biomedcentral.com)
- It also induces perturbation in mitochondrial membrane potential and enhances reactive oxygen species (ROS) generation in microglia. (biomedcentral.com)
Endogenous3
- However, whether HSP60 regulates endogenous IL-1β production is not known. (biomedcentral.com)
- Therefore, to probe the underlying mechanism, we elucidate the role of HSP60 in endogenous IL-1β production. (biomedcentral.com)
- HSP60 overexpression and knockdown experiment was done to elucidate the role of HSP60 in endogenous IL-1β production by microglia. (biomedcentral.com)
Overexpression1
- Overexpression of ClpP reduces αSyn-induced mitochondrial oxidative stress through enhancing the level of Superoxide Dismutase-2 (SOD2), and suppresses the accumulation of αSyn S129 phosphorylation and promotes neuronal morphology in neurons derived from PD patient iPS cells carrying αSyn A53T mutant. (springer.com)
Heat2
- Our previous study suggests the critical role of heat shock protein 60 (HSP60) in IL-1β-induced inflammation in microglia through TLR4-p38 MAPK axis. (biomedcentral.com)
- heat shock protein family D (Hsp60) me. (gsea-msigdb.org)
Functional3
- In this review, we discuss the functional cross talk of proteostasis and mitostasis in cellular homeodynamics and the impairment of mitochondrial quality control during ageing, cancer, and neurodegeneration. (hindawi.com)
- The CCT/TRiC complex is a type II chaperonin that undergoes ATP-driven conformational changes during its functional cycle. (bvsalud.org)
- Our findings provide new insights into the complex dynamics of chaperonins and their role in the functional folding cycle. (bvsalud.org)
Protein import1
- Implicated in mitochondrial protein import and macromolecular assembly. (abcam.cn)
Knockdown2
- Knockdown of HSP60 reduces the IL-1β-induced production of IL-1β both in vitro and in vivo. (biomedcentral.com)
- Also, we have shown for the first time that knockdown of HSP60 leads to decreased IL-1β production during Japanese encephalitis virus (JEV) infection, which eventually leads to decreased inflammation and increased survival of JEV-infected mice. (biomedcentral.com)
Dysfunction3
- Mitochondrial dysfunction has also been associated with ageing and most of the so-called age-related diseases [ 13 - 17 ]. (hindawi.com)
- Both α-Synuclein (αSyn) accumulation and mitochondrial dysfunction have been implicated in the pathology of Parkinson's disease (PD). (springer.com)
- Although the mechanisms underlying PD remain elusive, α-Synuclein (αSyn) accumulation and mitochondrial dysfunction have been recognized as major contributors [ 1 , 57 ]. (springer.com)
Survival1
- HSP60 predicts survival in advanced serous ovarian cancer. (gzsys.org.cn)
Pathway2
- HSP60 mediates microglial IL-1β production by regulating NLRP3 inflammasome pathway and reduction of HSP60 leads to reduction of inflammation in JEV infection. (biomedcentral.com)
- RAS genes in Saccharomyces cerevisiae: signal transduction in search of a pathway. (wikidata.org)
Experiment1
- The following product was used in this experiment: HSP60 Polyclonal Antibody from Thermo Fisher Scientific, catalog # PA5-143265. (thermofisher.com)
Sequence1
- A signal sequence domain essential for processing, but not import, of mitochondrial pre-ornithine carbamyl transferase. (mcmaster.ca)
Induction1
- The accumulation of OAS and the decrease in GSH are then presumed to regulate the expression of numerous genes, such as the induction of genes implicated in S uptake, assimilation and redistribution, improving the acquisition and the utilisation of S for plant growth [ 14 ]. (biomedcentral.com)
Family2
- This gene encodes a member of the chaperonin family. (thermofisher.com)
- This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. (thermofisher.com)
Species1
- Reactive oxygen species levels and mitochondrial membrane depolarization in N9 cells were analyzed by flow cytometry. (biomedcentral.com)
Expression3
- Compensating for the loss of ClpP in the substantia nigra of αSyn A53T mice by viral expression of ClpP suppresses mitochondrial oxidative damage, and reduces αSyn pathology and behavioral deficits of mice. (springer.com)
- Immunohistochemical expression of Hsp60 correlates with tumor progression and hormone resistance in prostate cancer. (gzsys.org.cn)
- HSP60 further activates NLRP3 inflammasome by elevating NLRP3 expression both at RNA and protein levels. (biomedcentral.com)
Interaction1
- Interaction between HSP60 and beta-catenin promotes metastasis. (gzsys.org.cn)
Function1
- Substantial evidence has shown that αSyn toxicity may directly disrupt mitochondrial function. (springer.com)
Bioinformatics1
- Gene fitness was determined by bioinformatics analysis from available datasets from genetic screenings. (biomedcentral.com)
Transcript1
- Two transcript variants encoding the same protein have been identified for this gene. (thermofisher.com)
Human2
- Synthetic peptide corresponding to Human Hsp60 aa 511-560. (abcam.cn)
- SDS-PAGE analysis: Lane 1: MWM, Lane 2: 1.0 µg of purified Human Hsp60 protein. (enzolifesciences.com)
Increases1
- Furthermore, HSP60 enhances caspase-1 activity and increases IL-1β secretion by microglia. (biomedcentral.com)
Organelle1
- Moreover, nonreversible mitochondrial damage targets the organelle to a specific autophagic removal, namely, mitophagy. (hindawi.com)
Complex2
- The dissociation of the Hsp60/pro-Caspase-3 complex by bis(pyridyl)oxadiazole copper complex (CubipyOXA) leads to cell death in NCI-H292 cancer cells. (gzsys.org.cn)
- Genes up-regulated through activation of mTORC1 complex. (gsea-msigdb.org)
Role1
- ATP-dependent Clp protease (ClpP), a mitochondrial matrix protease, plays an important role in regulating mitochondrial protein turnover and bioenergetics activity. (springer.com)
Promoter1
- These designs are created with VectorBuilder's standard backbones containing a ubiquitous promoter driving the ORF of your gene. (vectorbuilder.com)
Plant1
- In eukaryotes, Hsp60 is localized in the mitochondrial matrix while plant Hsp60 is localized in the chloroplast. (enzolifesciences.com)