Qproteome Mitochondria Isolation KitMembranesProteinsTissuesNeuronsDensity gradient centrifugationMammalianSaccharomycesPeroxisomesMass spectrometryEukaryotic cellsCastor bean endospermNucleusMetabolismCellularLiverLipidOrganellesDrosophilaRespiratory chainCharacterizationSucroseSynapticPhysiologyTherapeuticCalciumOxidativeFractionsStructuresProteinSeparationMembranePathologicalStem cellsUnderlying mechanismsFunctionalParametersCellsFormationDamageFunctionDepressionEnergyRoleBufferAnimalTransferTreatmentsImportantHumanFound
Qproteome Mitochondria Isolation Kit1
- 4472942). For mitochondria transfer generation: Mitochondria were harvested from HEK293T DsRed cells, LP351 PBMC, or LP298 PBMC using a Qproteome Mitochondria Isolation Kit (Qiagen, Cat. (nih.gov)
Membranes13
- With the optimal medium, the constituents of which are raffinose, versene (optional), dextran of high molecular weight, heparin, and AMP (optional), most of the mitochondria in the osmium-fixed pellet are apparently intact, and show the membranes characteristic of mitochondria as seen in cell sections. (rupress.org)
- Resuspend pellet in the isolation buffer (1:40, vol/vol) and spin for 15 min at 6500 x g, 4 o C. This will remove broken mitochondria and reduce ER membranes. (pancreapedia.org)
- Mitochondria-associated membranes (MAM) represent a region of the endoplasmic reticulum (ER) which is reversibly tethered to mitochondria. (wikipedia.org)
- These membranes are involved in import of certain lipids from the ER to mitochondria and in regulation of calcium homeostasis, mitochondrial function, autophagy and apoptosis. (wikipedia.org)
- In mammalian cells, formation of these linkage sites are important for some cellular events including: Mitochondria associated membranes are involved in the transport of calcium from the ER to mitochondria. (wikipedia.org)
- Isolation membranes are the initial step to form auto-phagosomes. (wikipedia.org)
- the ER stress have a key sensor enriched at the mitochondria-associated ER membranes (MAMs). (wikipedia.org)
- The mitochondria-associated ER membranes (MAMs), play role in cell death modulation. (wikipedia.org)
- Mitochindrial associated membranes associated with Alzheimer's disease have been reported to have an up-regulation of lipids synthesized in the MAMs juxtaposition and an up regulation of protein complexes present in the contact region between the ER and mitochondria. (wikipedia.org)
- Proteinase K finds application in in-vitro diagnostics, in the treatment of paraffin-embedded tissue sections during antibody labelling, extraction of mitochondria from hepatic cells, digestion of proteins from clinical tissue samples, and the analysis of the localization of enzymes on membranes. (pharmiweb.com)
- Many endoplasmic reticulum (ER) were transferred into a special "Chrysanthemum flower center" (CFC) in which several double-layer isolation membranes (IM) were formed and extended. (frontiersin.org)
- However, the excessive generation of ROS, such as super oxide anions (O 2 − ) and hydrogen peroxide (H 2 O 2 ) due to environmental stress results in significant oxidative damage to cell structures, such as DNA, the mitochondria and cell membranes ( 3 , 4 ). (spandidos-publications.com)
- Isolation of well purified mitochondria and mitochondria associated membranes (MAM) from animal tissues and cell cultures. (edu.pl)
Proteins4
- This transport is regulated with chaperones and regulatory proteins which control the formation of the ER-mitochondria junction. (wikipedia.org)
- Mitochondria contain their own DNA (mtDNA) and their own machinery for synthesizing RNA and proteins. (justia.com)
- This simultaneously denatures and digests the proteins, including the RNA, in the nuclei and mitochondria, leaving digested proteins and the pure, long-strand, high molecular weight genomic DNA in solution. (autogen.com)
- Proteins encoded by the mitochondrial genome or proteins encoded by the nuclear genome that are imported to and resident in the MITOCHONDRIA. (bvsalud.org)
Tissues2
- In fact, and differently from mammalian species, mitochondria from Drosophila melanogaster tissues are characterized by low amounts of SCs, despite the high metabolic demands and MRC activity shown by these mitochondria. (elifesciences.org)
- Isolation of Mitochondria from Mouse Tissues for Functional Analysis. (ucla.edu)
Neurons1
- DRP1 is required for proper cellular distribution of mitochondria, and in mutant neurons, mitochondria are largely absent from synapses, thus providing a genetic tool to assess the role of mitochondria at synapses. (nih.gov)
Density gradient centrifugation1
- The buoyant density of isolated mitochondria and glyoxysomes obtained from Neurospora crassa grown under different conditions was investigated by density-gradient centrifugation on Percoll. (microbiologyresearch.org)
Mammalian2
- WO 2016/135723 to the present inventors discloses mammalian bone marrow cells enriched with mitochondria for treatment of mitochondrial diseases. (justia.com)
- US 20130022666 provides compositions comprising a lipid carrier and mitochondria as well as methods of delivering exogenous mitochondria to a cell and methods of treating or reversing progression of a disorder associated with mitochondrial dysfunction in a mammalian subject in need thereof. (justia.com)
Saccharomyces1
- The proteome of Saccharomyces cerevisiae mitochondria. (nature.com)
Peroxisomes2
- Upon germination, mobilization of the storage reserves requires inter-organellar interplay of plastids, mitochondria, and peroxisomes to optimize growth for the developing seedling. (bvsalud.org)
- The data obtained were used to build a comprehensive metabolic model for plastids, mitochondria, and peroxisomes during storage reserve mobilization in castor bean endosperm. (bvsalud.org)
Mass spectrometry1
- Two‐dimensional electrophoresis of human placental mitochondria and protein identification by mass spectrometry: toward a human mitochondrial proteome. (nature.com)
Eukaryotic cells2
- The mitochondrion is a membrane bound organelle found in most eukaryotic cells, ranging from 0.5 to 1.0 μm in diameter. (justia.com)
- Mitochondria are found in nearly all eukaryotic cells and vary in number and location depending on the cell type. (justia.com)
Castor bean endosperm1
- Mitochondria and glyoxysomes from castor bean endosperm: enzyme constituents and catalytic capacity. (microbiologyresearch.org)
Nucleus2
- Our cells contain genetic information in two separate compartments - the nucleus and the mitochondria. (umu.se)
- Although most DNA is packaged in chromosomes within the nucleus, mitochondria also have a small amount of their own DNA. (medlineplus.gov)
Metabolism3
- Mitochondria are multifaceted organelles with key roles in anabolic and catabolic metabolism, bioenergetics, cellular signalling and nutrient sensing, and programmed cell death processes. (nature.com)
- Mitochondria perform numerous essential tasks in the eukaryotic cell such as pyruvate oxidation, the Krebs cycle and metabolism of amino acids, fatty acids and steroids. (justia.com)
- Metabolism of diethylhexyl phthalate by rats isolation and characterization of the urinary metabolites. (cdc.gov)
Cellular4
- Mitochondria are the organelles providing most of the cellular energy in form of adenosine triphosphate (ATP) in aerobic eukaryotes. (elifesciences.org)
- A tight ER-mitochondria contact site is integral to the mechanisms controlling cellular apoptosis and to inter-organelle Ca2 + signals. (wikipedia.org)
- This protein is found in a cellular compartment called the mitochondria, which is responsible for supplying energy to the rest of the cell and therefore plays an important role in metabolic processes. (elifesciences.org)
- Additional processes in which mitochondria are involved include heat production, storage of calcium ions, calcium signaling, programmed cell death (apoptosis) and cellular proliferation. (justia.com)
Liver8
- In view of the unsatisfactory appearance, under the electron microscope, of liver mitochondria isolated in isotonic sucrose medium, alternative media have been examined. (rupress.org)
- Isolation and analysis of fractions enriched in WrappER-associated mitochondria from mouse liver. (ulaval.ca)
- WrappER is a curved wrapping type of rough-ER that establishes extensive contacts with almost every mitochondria of the hepatocyte in the mouse liver. (ulaval.ca)
- Here, we describe a protocol for isolation of fractions enriched in wrappER-associated mitochondria from the mouse liver. (ulaval.ca)
- Kennedy, E. P. & Lehninger, A. L. Oxidation of fatty acids and tricarboxylic acid cycle intermediates by isolated rat liver mitochondria. (nature.com)
- Lardy, H. A. & Adler, J. Synthesis of succinate from propionate and bicarbonate by soluble enzymes from liver mitochondria. (nature.com)
- Isolation of rat liver lysosomes by isopycnic centrifugation in a metrizamide gradient. (rupress.org)
- The procedure for the isolation of rat liver lysosomes described in this paper is compared with the already existing methods. (rupress.org)
Lipid1
- Transport of phosphatidylserine into mitochondria from the ER for decarboxylation to phosphatidylethanolamine through the ER-mitochondria lipid which transform phosphatidic acid (PA) into phosphatidylserine (PS) by phosphatidylserine synthases 1 and 2 (PSS1, PSS2) in the ER and then transfers PS to mitochondria, where phosphatidylserine decarboxylase (PSD) transform into phosphatidylethanolamine (PE). (wikipedia.org)
Organelles1
- As an endurance athlete, the microscopic entities (organelles) located inside your muscle cells known as mitochondria are critical to your training and racing success. (trainingpeaks.com)
Drosophila1
- Disruption of the MRM2 Drosophila melanogaster orthologue leads to mitochondria-related developmental arrest. (nature.com)
Respiratory chain1
- This study presents valuable findings on the organization of respiratory chain complexes in mitochondria. (elifesciences.org)
Characterization3
- Work has also progressed in the characterization of the potassium transport system of E. coli and the investigation of the giant channels of mitochondria. (europa.eu)
- Isolation and chemical and genetic characterization will be attempted. (europa.eu)
- Nevertheless, a technique for isolating high-quality mitochondria is at present not out there in citrus fruit, stopping high-throughput characterization of mitochondrial capabilities. (procognia.com)
Sucrose1
- Isolation buffer: 250 mM sucrose, 1 mM EGTA, 10 mM Tris, adjusted pH to 7.4 with 1N HCl. (pancreapedia.org)
Synaptic1
- Our data suggest a specific role for mitochondria in regulating synaptic strength. (nih.gov)
Physiology1
- Mitochondria are affectionately referred to in almost every academic physiology textbook as the powerhouses of the cell. (trainingpeaks.com)
Therapeutic2
- The present invention provides stem cells enriched with healthy functional mitochondria, and therapeutic methods utilizing such cells for the alleviation of debilitating conditions, including aging, and age-related diseases as well as the debilitating effects of anti-cancer therapies in subjects in need thereof. (justia.com)
- The present invention relates to stem cells enriched with functional mitochondria, and therapeutic methods utilizing such cells to diminish the debilitating effects of various conditions, including aging and age-related diseases as well as the debilitating effects of anti-cancer therapy treatments. (justia.com)
Calcium2
- This interaction is important for rapid uptake of calcium by mitochondria through Voltage dependent anion channels (VDACs), which are located at the outer mitochondrial membrane (OMM). (wikipedia.org)
- Transfer of calcium from ER to mitochondria depends on high concentration of calcium in the intermembrane space, and mitochondrial calcium uniporter (MCU) accumulates calcium into the mitochondrial matrix for electrochemical gradient. (wikipedia.org)
Oxidative1
- Sugar supplies the basic building blocks (remember pyruvate and NADH) that the mitochondria use to produce ATP in the presence of oxygen (the whole process is called oxidative phosphorylation). (trainingpeaks.com)
Fractions1
- Further biochemical characterisation of the channels will be carried out using a traditional isolation approach involving solubilization and reconstitution of protein fractions (the most convenient functional assay being provided by the planar bilayer technique), leading to a study of the molecular biology of the channels. (europa.eu)
Structures1
- Separation of mitochondria from contaminating subcellular structures utilizing silica sol gradient centrifugation. (microbiologyresearch.org)
Protein2
- Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria. (nature.com)
- Using a gene editing tool to selectively substitute some of the building blocks, also known as amino acids, that make up the ATP5G1 protein revealed that improvements to the mitochondria were caused by switching specific amino acids. (elifesciences.org)
Separation1
- A good separation from mitochondria is obtained only when the L fraction if set down underneath the gradient. (rupress.org)
Membrane4
- Maybe it is the plasma membrane, the endoplasmic reticulum (ER) and the mitochondria. (wikipedia.org)
- MOMP is the process before apoptosis, which is accompanied to permeability of the inner membrane of the mitochondria (IMM). (wikipedia.org)
- Permeability transition pore (PTP) opening induces mitochondrial swelling and outer membrane of the mitochondria (OMM) rupture. (wikipedia.org)
- The method involves microinjecting heterologous mitochondria into an oocyte or embryonic cell wherein the heterologous mitochondria are capable of achieving at least normal levels of mitochondrial membrane potential in the oocyte or embryonic cell. (justia.com)
Pathological1
- These results highlight the potential of MSCs to modulate mitochondria in both inherited and pathological disease states. (frontiersin.org)
Stem cells1
- WO 2013/002880 describes compositions and methods comprising bio-energetic agents for restoring the quality of aged oocytes, enhancing oogonial stem cells or improving derivatives thereof (e.g., cytoplasm or isolated mitochondria) for use in fertility-enhancing procedures. (justia.com)
Underlying mechanisms1
- Thus, studies with isolated mitochondria can be a useful tool in understanding the underlying mechanisms of mitochondrial dysfunction in pancreatitis and pancreatic cancer. (pancreapedia.org)
Functional2
- This chapter presents detailed protocol developed by our group for isolation of pancreatic mitochondria and assessment of their functional parameters. (pancreapedia.org)
- Mitochondria maintain their functional activity for 4-5 h. (pancreapedia.org)
Parameters1
- To address this issue, we evaluated behavioral responses, assessed neuronal function and integrity, and conducted a comprehensive analysis of mitochondria-related parameters in selected brain regions of aged (24 months old) male rats supplemented with testosterone propionate (TP). (aging-us.com)
Cells5
- Our results indicated that terpinen-4-ol induced apoptosis through a mitochondria-mediated pathway in NSCLC cells and that the apoptosis elicited by terpinen-4-ol was p53 dependent. (hindawi.com)
- found that when this variant form of ATP5G1 was introduced into the cells of mice, their mitochondria was better at coping with stress conditions, such as low oxygen, low temperature and poisoning. (elifesciences.org)
- For our purposes we are going to focus on the muscle cells of the skeletal and cardiac (heart) systems but mitochondria populate many other cells of the human body. (trainingpeaks.com)
- Isolation and characteriza-tion of microbodies from Candida tropicalis pK 233 cells grown on n -alkanes. (microbiologyresearch.org)
- This lysis step releases the nuclei and mitochondria from the white blood cells, which are collected via centrifugation, leaving the RNA in the supernatant liquid (Figure 1b). (autogen.com)
Formation1
- But the ER- mitochondria contact site have markers, the auto-phagosome marker ATG14, and the auto-phagosome-formation marker ATG5, until the formation of auto-phagosome is complete. (wikipedia.org)
Damage1
- This damage results from a buildup of harmful molecules called reactive oxygen species, which are byproducts of energy production in mitochondria. (medlineplus.gov)
Function1
- In the nervous system, mitochondria SIRT3 deficiency would affect the normal function of the neuron [ 10 ]. (hindawi.com)
Depression1
- It can contribute to social isolation, depression , and loss of self-esteem. (medlineplus.gov)
Energy1
- The mitochondria take both of these ingredients, also called substrates, and use them to produce the energy (product) that makes your heartbeat and your muscles perform. (trainingpeaks.com)
Role1
- Mitochondria play a fundamental role in your performance as an endurance athlete. (trainingpeaks.com)
Buffer3
- Harvest rat or mouse pancreata, place immediately in ice-cold PBS, trim of fat and connective tissue, and wash with ice-cold isolation buffer to remove blood contamination. (pancreapedia.org)
- Place pancreas in 5 ml of isolation buffer, mince tissue into small pieces with scissors and homogenize using 5 to 6 up-and-down strokes at 600 rpm. (pancreapedia.org)
- Here, the white cell nuclei and mitochondria are treated with a denaturation buffer containing chaotropic salt and a protease. (autogen.com)
Animal1
- Several methods are available for the isolation of mitochondria from animal tissue and all of them are based on tissue disruption in an appropriate medium and subsequent differential centrifugation of homogenate (1,14,18). (pancreapedia.org)
Transfer1
- Caspase-activating factors induced by cytochrome C to bind to the IP3R, this will result in higher Ca2 + transfer from the ER to the mitochondria, amplifying the apoptotic signal. (wikipedia.org)
Treatments1
- With this method you also avoid having to use expensive RNase treatments to achieve a pure gDNA isolation. (autogen.com)
Important1
- Molly Breslin breaks down what mitochondria is, why it is important, and how you can increase it. (trainingpeaks.com)
Human2
- Also up increased connectivity between the ER and the mitochondria at MAM sites has been observed in human patients diagnosed with familial AD (FAD) by increase of the contact sites. (wikipedia.org)
- The Exiqon Mircury Rna Isolation Kit reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (micrornaprofile.com)
Found1
- Glucose (the form of sugar found in our blood stream) is repackaged inside the complex internal structure of the mitochondria into two key components: pyruvate and Nicotinic Adenine Dinucleotide (NADH). (trainingpeaks.com)