MitochondrialHydrazoneDysfunctionalCCCPAccumulationPhenotypesMembrane potentialMetabolismParkinConcentrationCellsVitroClearCellFoundTrifluoromethoxyAutophagyUncouplerReactive oxygenDegradationIntracellularUbiquitin ligaseOxidativeRespirationNeuronsOrganismalMitophagyMassMechanismFailureCell growthProductionHuman
Mitochondrial10
- Effects of mitochondrial uncouplers on intracellular calcium, pH and membrane potential in rat carotid body type I cells. (ox.ac.uk)
- This study evaluated the roles of PINK1 mitochondrial kinase, and Parkin E3 ubiquitin ligase in targeting depolarized mitochondria for degradation in vivo, using quantitative measurements of mitochondria in Drosophila nervous system. (sdbonline.org)
- The hypotheses was tested that impairment of mitochondrial quality control via suppression of PINK1 function should produce failures of turnover, accumulation of senescent mitochondria in the axon, defects in mitochondrial traffic, and a significant shift in the mitochondrial fission-fusion steady state. (sdbonline.org)
- Loss of PINK1 also produced specific, directionally balanced defects in mitochondrial transport, without altering the balance between stationary and moving mitochondria. (sdbonline.org)
- The Parkinson's disease genes pink1 and parkin , which encode a mitochondrially targeted protein kinase, and an E3 ubiquitin ligase, respectively, participate in a key mitochondrial quality-control pathway that eliminates damaged mitochondria. (sdbonline.org)
- The time effects of acidic pH e stress were determined with respect to tumor cell proliferation, phenotypic regulation, autophagic control, metabolic plasticity, mitochondrial network dynamics, and metastatic potentials. (biomedcentral.com)
- Mitochondrial condition in the carbonyl cyanide m-chlorophenyl hydrazine (CCCP) treated Parkin-expressing HeLa cells was compared with untreated cells using Mitophagy Detection Kit (MD01, MT02) and JC-1 MitoMP Detection Kit (MT09). (dojindo.com)
- Herein, effects of mitochondrial transplantation via different approaches in breast cancer were investigated. (biomedcentral.com)
- Fresh mitochondria isolated from homeoplasmic 143B osteosarcoma cybrids containing mitochondrial DNA (mtDNA) derived from health individuals (Mito) or mtDNA with the A8344G mutation (Mito 8344 ) were conjugated with cell-penetrating peptide Pep-1 (P-Mito) or not conjugated prior to cell co-culture. (biomedcentral.com)
- Furthermore, although there were no differences in energy metabolism after transplantation of normal mitochondria, metabolism was switched to the energetic and glycolytic phenotypes when the mitochondria were replaced with dysfunctional mitochondria, namely, Mito 8344 and P-Mito 8344 , due to dramatically induced glycolysis and reduced mitochondrial respiration, respectively. (biomedcentral.com)
Hydrazone1
- Both 2,4-dinitrophenol (DNP) and carbonyl cyanide p-trifluoromethoxyphenyl hydrazone (FCCP) caused an increase in [Ca2+]i which was largely inhibited by removal of extracellular Ca2+ or Na+, or by the addition of 2 mM Ni2+. (ox.ac.uk)
Dysfunctional2
- Attributed to the failure of neurons to clear dysfunctional mitochondria , loss of gene expression leads to loss of nigrostriatal neurons. (sdbonline.org)
- In C. elegans, UA prevented the accumulation of dysfunctional mitochondria with age and extended lifespan. (lsd1receptor.com)
CCCP1
- Outside of its effects on mitochondria, CCCP may also disrupt lysosomal degradation during autophagy. (wikipedia.org)
Accumulation1
- This shift in metabolic pathways is called "The Warburg Effect" and results in the accumulation of lactate regardless of oxygen availability (4). (researchsquare.com)
Phenotypes1
- The Pink1/parkin pathway plays a role in the quality control mechanism aimed at eliminating defective mitochondria, and the failure of this mechanism results in a reduced lifespan and impaired locomotor ability, among other phenotypes. (sdbonline.org)
Membrane potential1
- Red fluorescence indicates normal membrane potential or health mitochondria. (dojindo.com)
Metabolism3
- To test the first possibility, we measured the effect of UA on bacterial metabolism by measuring the growth of two Escherichia coli strains, OP50 and HT115, after treatment with UA. (lsd1receptor.com)
- Furthermore, in vitro culturing conditions can have a large effect on cell growth (11-13) and thus, cellular metabolism. (researchsquare.com)
- Mitochondria are dynamic organelles whose dynamic properties not only govern energy generation but also control the gateway to cancer based on their comprehensive regulation of energy metabolism, calcium homeostasis, redox regulation and apoptosis. (biomedcentral.com)
Parkin1
- To further evaluate the effects of the overexpression of the Bcl-2 homologue Buffy , this study analysed lifespan and climbing ability in both parkin -RNAi- and Pink1 -RNAi-expressing flies. (sdbonline.org)
Concentration1
- In contrast, treatment with EA at the same concentration had no effect on lifespan (Fig. 1b). (lsd1receptor.com)
Cells7
- We have therefore investigated their effects upon isolated type I cells. (ox.ac.uk)
- Mitochondria were visualized using TOM20 antibody (n=4, 50 cells counted for each replicate). (cov19longhaulfoundation.org)
- Mitochondria synthesize ATP using oxygen to produce necessary energy for living cells. (dojindo.com)
- HeLa cells treated with depolarizing reagent, carbonylcyanide-p-trifluoromethoxyphenylhydrazone (FCCP) were stained with JC-1 MitoMP Detection Kit. (dojindo.com)
- Untreated cells showed red fluorescence, while FCCP treated cells showed little red fluorescence. (dojindo.com)
- Whole mitochondria (approximately 10.5 μg/ml) were transported into MCF-7 breast cancer cells via passive uptake or Pep-1-mediated delivery. (biomedcentral.com)
- Compared with P-Mito, a small portion of Mito adhered to the cell membrane, and this was accompanied by a slightly lower fluorescent signal by foreign mitochondria in MCF-7 cells. (biomedcentral.com)
Vitro2
- A few studies have documented biological effects of urolithins in vitro, including antiproliferation in cancer cell models10, anti-inflammation11 and benefits on lipid metabolism12, although a clear biological pathway has not yet been described. (lsd1receptor.com)
- In this study we investigated the effects of over-expressing or silencing of sFRP4 in ovarian cancer lines with different chemosensitivity in an in vitro model. (biomedcentral.com)
Clear2
- We found a clear dose-response effect on lifespan when UA concentrations were increased from 10 to 50 µM (Fig. 1c). (lsd1receptor.com)
- Fragment: the majority of mitochondria were spherical (no clear length or width). (cov19longhaulfoundation.org)
Cell3
- 4. FCCP and DNP also depolarized type I cell mitochondria. (ox.ac.uk)
- These effects are unlikely to be caused by cell acidification. (ox.ac.uk)
- The transfer of whole mitochondria that occurs during cell contact has been found to support cancer progression. (biomedcentral.com)
Found2
- The biological effects of urolithins remain poorly characterized, despite wide-spread human exposure via the dietary consumption of their metabolic precursors, the ellagitannins, which are found in the pomegranate fruit, as well as in nuts and berries. (lsd1receptor.com)
- We found that UA had a very modest effect on the growth of these two strains (Supplementary Fig. 1a,b). (lsd1receptor.com)
Trifluoromethoxy1
- Using the mitochondrial uncoupler carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) and thapsigargin to empty intracellular Ca 2+ stores, we found that the initial increase originates from emptying of the endoplasmic reticulum and consequent influx of extracellular Ca 2+ , whereas the late increase originates primarily from mitochondria. (nih.gov)
Autophagy3
- Outside of its effects on mitochondria, CCCP may also disrupt lysosomal degradation during autophagy. (wikipedia.org)
- However, the effects of autophagy are highly contextual, and autophagic cell death (ACD) is emerging as an alternative paradigm of (stress- and drug-induced) cell demise. (lih.lu)
- Here, we found, in the Cln7 ∆ex2 mouse model of CLN7 disease, that failure in autophagy causes accumulation of structurally and bioenergetically impaired neuronal mitochondria. (nature.com)
Uncoupler1
- FCCP is a very potent uncoupler of oxidative phosphorylation in mitochondria. (bio-techne.com)
Reactive oxygen1
- Mitochondria are believed to be a major intracellular source of reactive oxygen species (ROS) as a byproduct of the respiratory chain-mediated transfer of electrons to oxygen and its incomplete reduction. (biomedcentral.com)
Degradation1
- This study evaluated the roles of PINK1 mitochondrial kinase, and Parkin E3 ubiquitin ligase in targeting depolarized mitochondria for degradation in vivo, using quantitative measurements of mitochondria in Drosophila nervous system. (sdbonline.org)
Intracellular1
- Less well appreciated is the fact that mitochondria integrate environmental and intercellular as well as intracellular signals to modulate function. (nih.gov)
Ubiquitin ligase1
- The Parkinson's disease genes pink1 and parkin , which encode a mitochondrially targeted protein kinase, and an E3 ubiquitin ligase, respectively, participate in a key mitochondrial quality-control pathway that eliminates damaged mitochondria. (sdbonline.org)
Oxidative1
- Since mitochondria are involved in tumorigenesis through ROS production and modulation of oxidative metabolism, we investigated the biological effects of pomegranate on cellular redox state, proliferation and metabolism in the breast cancer cell line MDA-MB-231 (MDA). (biomedcentral.com)
Respiration2
- Additionally, it enhanced mitochondria respiration, and decreased glycolysis at high concentrations, inhibiting at the same time cell proliferation. (biomedcentral.com)
- Instead, we show that pyrvinium localizes to mitochondria and induces apoptosis by inhibiting mitochondrial respiration. (oncotarget.com)
Neurons1
- Attributed to the failure of neurons to clear dysfunctional mitochondria , loss of gene expression leads to loss of nigrostriatal neurons. (sdbonline.org)
Organismal2
- Because mitochondria function in an organismal milieu, there is need for assays capable of rapidly assessing mitochondrial health in vivo. (nih.gov)
- While these effects at the organismal level were interesting in a pest control context, the effects at the organ or tissue level and also immune effects have not been investigated yet. (bvsalud.org)
Mitophagy1
- Collectively, these data suggest that early mitochondrial dysfunction and HMOX1 overactivation synergize to trigger lethal mitophagy, which contributes to the cell killing effects of AT 101 in glioma cells. (lih.lu)
Mass1
- AT 101 rapidly triggered mitochondrial membrane depolarization, engulfment of mitochondria within autophagosomes and a significant reduction of mitochondrial mass and proteins that did not depend on the presence of BAX and BAK1. (lih.lu)
Mechanism1
- Additionally, based on the observed mechanism of action, a cumulative neurotoxic effect of PBDEs and ortho -substituted polychlorinated biphenyls on [Ca 2+ ] i cannot be ruled out. (nih.gov)
Failure1
- However, parental RNAi of FucT6 led to full failure in the hatching of eggs, and this effect was maternally mediated. (bvsalud.org)
Cell growth2
- The lectin presented a biostatic effect by reducing the cell growth and inducing cell agglutination, suggesting an interaction with glycans on the cell surface. (bvsalud.org)
- Conversely, freezing procedure, though retaining the antioxidant and cell-growth inhibitory property, elicited an opposite effect on the metabolic profile as compared with fresh extract. (biomedcentral.com)
Production1
- Mitochondria are critical for their role in ATP production as well as multiple nonenergetic functions, and mitochondrial dysfunction is causal in myriad human diseases. (nih.gov)
Human1
- Increasing concentrations of brominated flame retardants, in particular polybrominated diphenyl ethers (PBDEs), in the environment, human food chain, and human tissues raise concern about possible neurotoxic effects. (nih.gov)