• Recently, we have found that a number of microRNAs (miRNAs) and proteins are involved in the response to acupuncture therapy in hypertensive rats. (hindawi.com)
  • MicroRNAs (miRNAs) is a large family of endogenous, small noncoding RNAs with 20-25 nucleotides that have emerged as key posttranscriptional regulators of gene expression in mammals, bacteria, and plants [ 1 - 4 ]. (hindawi.com)
  • MicroRNAs (miRNAs) are small non-coding RNAs that function as novel gene expression regulators at the post-transcriptional level. (engreen.com.cn)
  • DRB4 was purified from (fig. guided through microRNAs (miRNAs) and small interfering RNAs (siRNAs) ((and through RNA-dependent DNA methylation mechanisms ((have reduced siRNA production from sense and inverted-repeat transgenes. (researchhunt.com)
  • MicroRNAs (miRNAs) and Twist1-induced epithelial-mesenchymal transition (EMT) in cancer cell dissemination are well established, but the involvement of long noncoding RNAs (lncRNAs) in Twist1-mediated signaling remains largely unknown. (biomedcentral.com)
  • Thus, it would be of interest to identify molecular biomarkers which indicate predisposition to the development of HD, and as microRNAs (miRNAs) circulate in bio-fluids they would be particularly useful biomarkers. (fortuneonline.org)
  • microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. (nih.gov)
  • SncRNAs are less than 200 nt in length, consisting of microRNAs (miRNAs), Piwi-interacting RNAs (piRNAs), endogenous small interfering RNAs (endo-siRNAs or esiRNAs) and transcription initiation RNAs (tiRNAs) [ 6 ]. (ijbs.com)
  • MicroRNAs (miRNAs) certainly are a course of noncoding little RNAs that play important roles within the modulation of varied biological procedures through straight binding towards the 3 untranslated area (3-UTR) of focus on genes, which leading to posttranscriptional inhibition and mRNA cleavage [16, 17]. (opioid-receptors.com)
  • MicroRNAs (miRNAs) have been established as important regulators of gene expression in the mammalian ovary. (appliedbioinfo.com)
  • Transcriptome-wide identification of RNA-binding protein and microRNA target sites by PAR-CLIP. (eurekaselect.com)
  • 2. MicroRNA (miRNA) MiRNAs are noncoding little RNA substances that become posttranscriptional regulators. (bio-biz-navi.com)
  • More importantly, circRNAs serve as microRNA (miRNA) sponges and crucial regulators of gene expression. (ijbs.com)
  • Nevertheless, only a few of the almost 60,000 lncRNAs have been characterized, and little is known about their diverse functions (transcriptional interference, chromatin remodeling, splicing, translation regulation, interaction with miRNAs and siRNAs, and mRNA degradation). (wikipedia.org)
  • Because alternative RNA splicing allows different types of mRNA molecules to be created from a single gene, it generates the diversity of protein function and structure that is essential to complex organisms. (genomicglossaries.com)
  • RNA-like oligonucleotides that are complementary to a portion of a target mRNA molecule. (genomicglossaries.com)
  • More specifically, antisense oligonucleotides that are useful as reagents for target validation , or as drugs, are engineered molecules that differ from natural RNA but that have a base sequence that is recognized as being complementary to a very specific mRNA sequence. (genomicglossaries.com)
  • A complementary RNA sequence that binds to a naturally occurring (sense) mRNA molecule, thus blocking its translation. (genomicglossaries.com)
  • For example, an individual miRNA may regulate multiple mRNAs, and in contrast, an individual gene may also be regulated by multiple miRNAs, thus representing a complex network of miRNA-mRNA interactions. (biomedcentral.com)
  • Nuclear mRNA transcripts acquire a protein coat composed of cap- and RNA-binding proteins that allow nuclear export and dictate the functional program of cytoplasmic mRNAs ( Moore, 2005 ). (rupress.org)
  • Different classes of RNA granules share some protein components and may use similar mechanisms to regulate mRNA translation/decay. (rupress.org)
  • Among the strands, the older miRNA strand or information strand, is certainly packed in the RNA induced silencing complicated (RISC), and directs RISC to the mark mRNA, where in fact the complicated hybridizes to (partly) complementary sequences leading to cleavage or translational inhibition of the mark mRNA. (bio-biz-navi.com)
  • We explored a large HD miRNA-mRNA expression dataset (GSE65776) using bioinformatics and machine learning (ML) techniques. (fortuneonline.org)
  • The new class of plant small peptide regulators was recently shown to be encoded by primiRNA transcripts which can be transported to cytoplasm in unprocessed mRNA-like form. (fortunepublish.com)
  • The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. (nih.gov)
  • RNA-seq experiments generate reads derived not only from mature RNA transcripts but also from pre-mRNA. (nature.com)
  • Here we present a computational approach called exon-intron split analysis (EISA) that measures changes in mature RNA and pre-mRNA reads across different experimental conditions to quantify transcriptional and post-transcriptional regulation of gene expression. (nature.com)
  • In general, these protocols aim to enrich for mature mRNA by selection of polyadenylated RNA or by depletion of ribosomal RNA. (nature.com)
  • MiRNA-mRNA target prediction was performed using miRWALK and c-Met protein levels were analyzed by immunohistochemistry. (biomedcentral.com)
  • Comprehensive lncRNA-mRNA-miRNA co-expression pattern analysis was also performed. (biomedcentral.com)
  • In line with aberrant expression of certain lncRNAs in tumors, the expression of miRNA and mRNA targets showed systematic alterations. (biomedcentral.com)
  • Here, we highlight that an important RNA sequence region, encompassing an exon-intron hairpin loop (also called IDX-rasISS1), of the H-Ras pre-mRNA may encode an ncRNA that regulates p68 RNA helicase. (lidsen.com)
  • The biosynthesis of BC200 RNA occurs at the cell body of a neuron and requires upstream promoter elements, downstream internal promoter elements (intragenic A and B boxes), at least two transcription factor binding sites, a TATA-like sequence, TATA-box binding protein (TBP), and RNA polymerase III. (wikipedia.org)
  • 87(5): 1663-1667] Specifically, the investigators started by priming whole cerebellar RNA with a synthetic oligonucleotide containing a T7 RNA polymerase promoter sequence. (genomicglossaries.com)
  • After second- strand cDNA synthesis, T7 RNA polymerase was used to generate aRNA. (genomicglossaries.com)
  • MiRNAs are usually generated by RNA polymerase II. (bio-biz-navi.com)
  • miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. (nih.gov)
  • As a long non-coding RNA, it belongs to a family of RNA transcripts that are not translated into protein (ncRNAs). (wikipedia.org)
  • As a long non-coding cytoplasmic RNA, BC200 RNA is a part of the largest group of non-coding transcripts in the human genome, which is more prevalent than protein coding genes. (wikipedia.org)
  • Ghini F, Rubolino C, Climent M, Simeone I, Marzi MJ, Nicassio F. Endogenous transcripts control miRNA levels and activity in mammalian cells by target-directed miRNA degradation. (eurekaselect.com)
  • Although thousands of sncRNA genes are known to exist in the human genome, no single database provides searchable, unified annotation, and expression information for full sncRNA transcripts and mature RNA products derived from these larger RNAs. (lisanwanglab.org)
  • Because BC200 RNA acts as a translational regulator, it is then transported to the dendrites to bind to specific proteins involved in translation and inhibit their activity (see next section). (wikipedia.org)
  • Our bioinformatics study suggests an association between these miRNAs and proteins, which include miR-339 and sirtuin 2 (Sirt2). (hindawi.com)
  • Bioinformatic algorithms allowing predicting of biomolecular folding for proteins, peptides, and RNAs, even though sometimes successful, have all their limitations. (biosyn.com)
  • RNA molecules in solution may adopt secondary structures that are only partially determined by thermodynamics since RNA molecules can undergo conformational changes during interaction with other RNAs, RNA binding proteins or RNA binding peptides. (biosyn.com)
  • Since RNA-binding proteins (RBPs) are key players in the post-transcriptional regulation of gene expression precise knowledge of their binding sites is critical for determining their molecular function and for understanding their roles in cell development and disease. (biosyn.com)
  • The principal transcript (pri-miRNA) is certainly processed with the RNase III enzyme Drosha, in collaboration with double-stranded (ds) RNA-binding proteins DGCR8 right into a ~60 pre-miRNA hairpin. (bio-biz-navi.com)
  • Shi D, Cheng X, Saquet A, Grifone R. RNA-Binding Proteins and Inner Ear Hair Cell. (encyclopedia.pub)
  • RNA-Binding Proteins and Inner Ear Hair Cell" Encyclopedia , https://encyclopedia.pub/entry/30958 (accessed December 05, 2023). (encyclopedia.pub)
  • RNA-binding proteins (RBPs) regulate gene expression at the post-transcriptional level. (encyclopedia.pub)
  • LIN28 (LIN28A and LIN28B) proteins are highly conserved small cytoplasmic RNA-binding proteins (RBPs) that function as pluripotency factors, regulating the transition from self-renewal to a differentiated cell fate [ 1 ] . (encyclopedia.pub)
  • Previously, non-coding RNAs (ncRNAs), including micro-RNAs (miRNAs) and other long non-coding RNAs (lncRNAs), have been generally considered unable to encode proteins both in plants and animals [1-9]. (fortunepublish.com)
  • Cytoplasmic -catenin is definitely managed by the degradation complicated made up of adenomatosis polyposis coli (APC), Axin, proteins phosphatase 2A (PP2A), glycogen synthase kinase 3 beta (GSK3) and casein kinase 1 (CK1) [6C9]. (opioid-receptors.com)
  • According to experimental data, lncRNAs can interact with DNA, RNA and also with proteins and can either promote or inhibit transcription [ 10 ]. (biomedcentral.com)
  • hundreds of human genes encode signal proteins, cell-surface receptors, cell adhesion proteins, or ion channels that are either not present in yeast or present in much smaller numbers. (catsboard.com)
  • miRNAs are transcribed as ~70 nucleotide stem-loop precursors and subsequently processed by the cytoplasmic RNase-III type enzyme Dicer to generate ~22 nucleotide mature products which can target and modulate gene expression by inhibiting translation and/or inducing degradation of target mRNAs [ 4 , 6 , 7 ]. (hindawi.com)
  • The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA. (hindawi.com)
  • miRNAs act as adaptors that employ a silencing complex to target mRNAs by selective base pairing, primarily in the 3′-untranslated region (3′-UTR). (hindawi.com)
  • In pets, mature miRNAs usually do not need complete complementarity with their focus on mRNAs, enabling these to bind to and stop translation of many. (bio-biz-navi.com)
  • We sought sets of features (mRNAs or miRNAs) to predict HD or WT samples from aged or young mouse cortex samples, and we asked if a set of features could predict predisposition to HD or WT genotypes by training on aged samples and testing using the young samples. (fortuneonline.org)
  • Mechanistically, MS-444 disrupted HuR cytoplasmic trafficking and released ARE-mRNAs for localization to P-bodies, but did not affect total HuR expression levels. (oncotarget.com)
  • Finally, alternative splicing microarrays containing apoptosis targets were incubated to verify whether pre-mRNAs other than H-Ras could also present a similar hairpin loop structure regulated by p68 RNA helicase. (lidsen.com)
  • Molecular interactions involve several types of entities including Long non-coding RNAs (lncRNAs) and Small non-coding RNAs (sncRNAs), further divided into subclasses shortly called biotypes. (biomedcentral.com)
  • The lncRNAs have a broader spectrum of functions [ 2 , 3 ] such as regulation of transcription, RNA processing, nuclear-cytoplasmic transport, translation control and modulation of chromatin structure and are, therefore, a potential new class of cancer therapeutic targets [ 4 ]. (biomedcentral.com)
  • Indeed, we demonstrated that in Rhabdomyosarcoma there is an augmented production of a class of peculiar lncRNAs, the covalently closed circular RNAs. (uniroma1.it)
  • ncRNAs can be classified into long ncRNAs (lncRNAs) and small ncRNAs (sncRNAs) [ 4 ]. (ijbs.com)
  • Long non-coding RNAs (lncRNAs) play a fundamental role in colorectal cancer (CRC) development, however, lncRNA expression profiles in CRC and its precancerous stages remain to be explored. (biomedcentral.com)
  • MiRNA targets of lncRNAs were predicted with miRCODE and lncBase v2 algorithms and miRNA expression was analyzed on miRNA3.0 Array data. (biomedcentral.com)
  • DsiRNAs are 27mer duplex RNAs that demonstrate increased potency in RNA interference compared to traditional, 21mer siRNAs. (idtdna.com)
  • Long dsRNAs are degraded by the endoribonuclease Dicer into small effector molecules called siRNAs (small interfering RNAs). (idtdna.com)
  • IDT DsiRNAs are chemically synthesized 27mer RNA duplexes that are optimized for Dicer processing and show increased potency when compared with 21mer siRNAs [ 7-8 ]. (idtdna.com)
  • Specific tertiary structure types are observed for transfer RNA (tRNA), ribosomal RNA (rRNA), small nuclear RNA (snRNA), certain introns, and ribozymes. (biosyn.com)
  • The Filipowicz lab reported enrichment of mammalian Dicer at ribosomal RNA loci, suggesting a possible role for Dicer in maintaining integrity of ribosomal DNA arrays. (prolekarniky.cz)
  • Noncoding RNAs (ncRNAs) are a large and diverse class of transcribed RNA molecules and participate in many biological processes [ 3 ]. (biomedcentral.com)
  • Non-coding RNAs (ncRNAs), for a long time believed to be non-functional, are emerging as one of the most important and large family of gene regulators and key elements for genome maintenance. (biomedcentral.com)
  • The availability of omics repositories represents a powerful resource for the discovery of interactions among non coding RNAs (ncRNAs). (biomedcentral.com)
  • Circular RNAs (circRNAs), a novel type of non-coding RNAs (ncRNAs), are ubiquitously expressed in eukaryotic cells during post-transcriptional processes. (ijbs.com)
  • However, it has been proven that more than 62% of genomic DNA serves as a template for transcription, which indicates that there are abundant non-coding RNAs (ncRNAs) in human transcriptome [ 2 , 3 ]. (ijbs.com)
  • Non-coding RNAs (ncRNAs) have emerged as one of the most abundant regulatory molecules. (lidsen.com)
  • The discovery of miRNAs has revolutionized our comprehension of the regulation of gene expression. (hindawi.com)
  • Endogenous antisense RNAs function as regulators of gene expression by a variety of mechanisms. (genomicglossaries.com)
  • Therapeutic tiny or short modified antisense oligonucleotides (Tiny ASOs) can block gene expression or modulate splice-switching! (biosyn.com)
  • The characterization of RNA and RNA interactions is closely related to transcription, for example, gene expression levels are investigated within a biological context. (biosyn.com)
  • Cytoplasmic RNA granules in germ cells (polar and germinal granules), somatic cells (stress granules and processing bodies), and neurons (neuronal granules) have emerged as important players in the posttranscriptional regulation of gene expression. (rupress.org)
  • Despite the well-known fact that primiRNAs are acting as non-protein-coding RNAs, the published data suggest that, in the plant genomes, some pri-miRNAs can also be found in polysomes, and the expression of these miRNA precursors may results in formation of micropeptides which may be involved in regulation of gene expression. (fortunepublish.com)
  • In mammals, Dicer processing occurs as a complex with the RNA-binding protein TRBP. (idtdna.com)
  • The nascent siRNA associates with Dicer, TRBP, and Argonaut (Ago2) to form the RNA-induced silencing complex (RISC), which mediates gene silencing (Figure 1) [ 3 ]. (idtdna.com)
  • Dicer-substrate RNAi methods take advantage of the link between Dicer and RISC loading that occurs when RNAs are processed by Dicer. (idtdna.com)
  • DsiRNAs are chemically synthesized, 27 nt RNA duplexes that are optimized for Dicer processing and are ideal for small-scale in vitro applications. (idtdna.com)
  • Cytoplasmic Dicer is a key component of the canonical micro (mi)RNA biogenesis pathway. (prolekarniky.cz)
  • employed an endogenously HA-tagged Dicer knock-in mouse cell line to show that Dicer is exclusively cytoplasmic. (prolekarniky.cz)
  • This paper challenges several studies reporting various RNA metabolic functions of Dicer in human nuclei. (prolekarniky.cz)
  • The endoribonuclease Dicer recognises and processes double-stranded (ds)RNA substrates of various origins into small non-coding (nc)RNA [ 1 ]. (prolekarniky.cz)
  • Interestingly, Dicer depletion in human cells caused defects in precursor messenger (pre-m)RNA processing [ 8 ]. (prolekarniky.cz)
  • The latter two studies conclude that Dicer association with chromatin may be mediated by the localised production of dsRNA, which is processed into endogenous small interfering (endo-si)RNA to mediate heterochromatin formation by recruitment of G9a methyltransferase in a Dicer-dependent manner. (prolekarniky.cz)
  • This nuclear pre-miRNA is certainly then transported in to the cytoplasma by exportin 5/Ran-GTP and cleaved with the cytoplasmic RNase III Dicer to create an imperfect ds RNA of 21C25 nucleotides. (bio-biz-navi.com)
  • The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. (nih.gov)
  • Not with standing that the biogenesis and function of miRNAs are well-understood in eukaryotes, little is known about RNA virus-encoded miRNAs. (engreen.com.cn)
  • This review summarizes the latest results on virus-encoded miRNAs and MK-0679 their referred to features and briefly discusses the potential of antiviral miRNA being a book therapeutic technique in combating pathogen attacks. (bio-biz-navi.com)
  • We apply EISA to 17 diverse data sets to show that most intronic reads arise from nuclear RNA and changes in intronic read counts accurately predict changes in transcriptional activity. (nature.com)
  • Synthetic antisense RNAs are used to effect the functioning of specific genes for investigative or therapeutic purposes. (genomicglossaries.com)
  • It turned out that four peptides, encoded by a number of long non-coding RNAs, have a length of 11 to 32 residues and are necessary for embryonic development of flies [13, 14]. (fortunepublish.com)
  • Then, generation and function analyses were conducted on one of the candidate miRNAs, BmCPV-miR-1, in the BmN cells and the silkworm larvae by RNA interference, quantitative PCR, dual-luciferase assay. (engreen.com.cn)
  • What is RNA interference? (idtdna.com)
  • RNA interference is a conserved pathway common to plants and mammals, where double-stranded RNAs (dsRNAs) suppress expression of genes with complementary sequences [ 1-2 ]. (idtdna.com)
  • For these reasons, infections are suffering from different strategies, among which include the posttranscriptional legislation of both mobile and viral gene expressions through modulating the host's RNA-interference (RNAi) equipment. (bio-biz-navi.com)
  • Moreover, a similar effect on p68 RNA helicase-mediated interference is observed during the upregulation of the hairpin loop. (lidsen.com)
  • Noncoding RNAs such as circular RNAs (circRNAs) are abundant in the human body and influence the occurrence and development of various diseases. (biomedcentral.com)
  • We demonstrated that YTHDC1, the reader of the RNA epitranscriptomic modification N6 methyladenosine and the RNA helicase DDX5 are able to act in complex to regulate this process in this cancer type. (uniroma1.it)
  • The precise mechanism by which Lin28B and let7 miRNA regulate mTORC1 activity in cochlear epithelial cells awaits further investigation. (encyclopedia.pub)
  • Initially, two observations prompted the present study: i) previous results revealed down-regulation of p68 RNA helicase resulting from overexpression of the IDX hairpin loop in HeLa cells, and ii) the secondary structure of the IDX hairpin loop resembles pri-miRNAs, implying that an miRNA could be processed from the hairpin loop-containing pri-miRNA and regulate 68 RNA helicase. (lidsen.com)
  • Based on miRBase version 21 released in June 2014 ( http://www.mirbase.org/ ), there are 1,881 miRNA precursors and 2,588 mature miRNAs in humans. (hindawi.com)
  • RNAs are single-stranded molecules and the unlinked monomer building blocks are known as nucleotides. (biosyn.com)
  • Fig. 4: Localization of transposon RNAs to cytoplasmic RGs. (nature.com)
  • In vivo treatment of MS-444 inhibited HuR cytoplasmic localization and decreased COX-2 expression in tumors. (oncotarget.com)
  • This indicates that an inhibitory feedback mechanism acting on the p68 RNA helicase is mediated by higher structural levels of the hairpin-loop. (lidsen.com)
  • Furthermore, RNAi assays containing the hairpin loop as an miRNA precursor were conducted, using the pTer vector, to explore the effects on 68 RNA helicase expression levels. (lidsen.com)
  • These RNAi analyses were quantified by Western blots (using anti-p68 RNA helicase and anti-EIF 2 α ) and Fluc/Rluc 3'UTRs/CDS assays. (lidsen.com)
  • Cellular RNAs are regulated at multiple stages, including transcription, RNA maturation and degradation. (nature.com)
  • Overexpression of the RNA-binding protein HuR (ELAVL1) occurs during colon tumorigenesis and is abnormally present within the cytoplasm, where it post-transcriptionally regulates genes through its interaction with 3'UTR AU-rich elements (AREs). (oncotarget.com)
  • There is a deletion of sequences between -100 and -1 in the DNA that blocks transcription activity, revealing that the transcription complex must interact with this 100-bp sequence of the upstream region for proper synthesis of BC200 RNA. (wikipedia.org)
  • In addition to upstream elements, there is an upstream TATGAAA sequence (similar to TATA box sequence) at positions -28 to -22 which, when deleted, compromises transcription, revealing this TATA-like sequence as another critical player in the synthesis of BC200 RNA. (wikipedia.org)
  • The topics will include transcription, RNA processing and metabolism, non-coding RNAs, RNA in therapy and medicine. (gov.pl)
  • Brain cytoplasmic 200 long-noncoding RNA (or BC200 lncRNA) is a 200 nucleotide RNA transcript found predominantly in the brain with a primary function of regulating translation by inhibiting its initiation. (wikipedia.org)
  • moreover, we have described the functions of the multiple elements generated by the complex MN2 locus: two miRNAs (miR-384-5p and miR-325-3p) and a cytoplasmic lncRNA lncMN2-203 that synergistically cooperate to favor motor neurogenesis. (uniroma1.it)
  • For example, a small peptide that is encoded by lncRNA HOXB-AS3 inhibits oncogenesis by regulating alternative splicing and metabolic reprogramming of colon cancer cells [5-7, 17]. (fortunepublish.com)
  • Circular RNAs (circRNAs), a novel member of the lncRNA kingdom, are generated by non-sequential backsplicing of exons, introns or a combination of both [ 7 - 9 ]. (ijbs.com)
  • Our results provide new evidence supporting the hypothesis that RNA viruses could generate miRNAs to modulate antiviral host defense. (engreen.com.cn)
  • Wang M, Yu F, Wu W, Zhang Y, Chang W, Ponnusamy M, Wang K, Li P. Circular RNAs: A novel type of non-coding RNA and their potential implications in antiviral immunity. (ijbs.com)
  • Being an integral part of the eukaryotic transcriptome, miRNAs are regarded as vital regulators of diverse developmental and physiological processes. (eurekaselect.com)
  • The program will be broadly aligned with the RNA Biology in prokaryotic and eukaryotic systems. (gov.pl)
  • Bombyx mori cypovirus (BmCPV) is a double-stranded RNA virus with a segmented genome that causes cytoplasmic polyhedrosis disease in silkworm larvae. (engreen.com.cn)
  • miRNAs are predicted to control the activity of more than 30% of human genes [ 5 ]. (hindawi.com)
  • Finally, genes used to train our miRNA-based predisposition model were mined from HD patient bio-fluid samples. (fortuneonline.org)
  • The TATA-box binding protein (TBP) binds here, and when inhibited, BC200 RNA levels decrease, indicating that the 100 base pair region and TBP are critical players in the biosynthesis of BC200 RNA. (wikipedia.org)
  • Over the last decades, a variety of RNA methods have been developed for the study of RNA-DNA, RNA-RNA, and RNA-protein interactions including RNA complexes with ligand molecules. (biosyn.com)
  • RNA molecules are functionally diverse and involved in many cellular processes such as catalysis, ligand binding, and protein recognition. (biosyn.com)
  • The mapping of RNA-protein or RNA-RNA interactions by protein pull-down or affinity pull-down methods allow studying RNA structures, as well as RNA-protein, and RNA-RNA interactions. (biosyn.com)
  • Furthermore, pumilio homolog 2 (PUM2), a RNA-binding protein, mediated the packaging of miRNA-130a into exosomes. (dovepress.com)
  • The authors showed that transfection of exogenous small interfering (exo-si)RNA triggers silencing of a subset of protein-coding gene promoters. (prolekarniky.cz)
  • Secretory carrier membrane protein 4 (SCAMP4) was predicted to be subject to posttranscriptional gene regulation mediated by small RNA, by annotating small RNA reads mapped to exonic regions in the pig genome. (appliedbioinfo.com)
  • RNA molecules which hybridize to complementary sequences in either RNA or DNA altering the function of the latter. (genomicglossaries.com)
  • RNA molecules are structural flexible and can adopt different structures. (biosyn.com)
  • RNA molecules are generally folded into compact and defined tertiary structures. (biosyn.com)
  • Histology, whole exome and RNA sequencing revealed a high concordance with the primary patient's tumor profile, human leukocyte-antigen characteristics and specific metabolic pathway signatures. (bvsalud.org)
  • Although RNA-seq mostly generates reads that map to exons, it also captures less abundant intronic sequences 6 . (nature.com)
  • RNAi can be performed in mammalian cells using short RNAs, which generally do not induce IFN responses. (idtdna.com)
  • The interaction of circHERC1 with FOXO1 was verified by RNA pull-down, RNA immunoprecipitation (RIP) and western blot assays. (biomedcentral.com)
  • Moreover, the role of RNA interaction is often deregulated in pathological context. (uniroma1.it)
  • Clearly, miRNA activity is kept in check by various regulatory mechanisms that control their biogenesis and decay pathways. (eurekaselect.com)
  • Little is known about their biogenesis and alteration in cancer. (uniroma1.it)
  • This study indicates that CAFs-derived exosomal miRNA-130a may be a potential therapeutic target for cisplatin resistance in NSCLC. (dovepress.com)
  • Here, we examine the therapeutic potential of targeting HuR using MS-444, a small molecule HuR inhibitor. (oncotarget.com)
  • We have characterized the first SG transcriptome in human neural system, and we have found that in presence of mutant FUS their physical properties and RNA composition are altered. (uniroma1.it)
  • EISA reveals both transcriptional and post-transcriptional contributions to expression changes, increasing the amount of information that can be gained from RNA-seq data sets. (nature.com)
  • Taken together, we help to elucidate a mechanism of post-transcriptional regulation by miRNA in the mammalian ovary. (appliedbioinfo.com)
  • A previous screen of small RNA in the porcine ovary identified the down regulation of miR-574 during oocyte maturation, although its role during this process was not established. (appliedbioinfo.com)
  • Inhibiting this miRNA during in vitro maturation (IVM) increased HAS2 levels along with several markers of oocyte quality. (appliedbioinfo.com)
  • Additionally, a heterologous miRNA microarray method was also used to identify miRNA expressed in the oocyte during in vitro maturation. (appliedbioinfo.com)
  • Similar to the expression analysis of other species, these data demonstrate dynamic expression regulation of multiple classes of non-coding RNA during oocyte maturation and development to the blastocyst stage. (appliedbioinfo.com)
  • Viral RNA was predominantly restricted to neurons, particularly in the brain stem, cerebellum (Purkinje cells), and cervical spinal cord. (cdc.gov)
  • This review provides a bird's eye view of miRNAs expressed in different subcellular locations, thus affecting the gene regulatory pathways therein. (eurekaselect.com)
  • Further mechanistic research indicated that circHERC1 acted as a competing endogenous RNA of miR-142-3p to relieve the repressive effect of miR-142-3p on its target HMGB1, activating the MAPK/ERK and NF-κB pathways and promoting cell migration and invasion. (biomedcentral.com)
  • If these ceRNAs possess many miRNAs response elements (MREs) and are expressed at high enough levels, they act to sequester miRNAs [ 10 ]. (biomedcentral.com)
  • Unlike linear RNAs, circRNAs form covalent-closed continuous loops without 5' to 3' polarities and poly (A) tails. (ijbs.com)
  • CircRNAs were first identified in RNA viruses in the 1970s [ 12 - 14 ]. (ijbs.com)
  • We review the relationship between different classes of these granules and discuss how spatial organization regulates messenger RNA translation/decay. (rupress.org)
  • Three recent studies based on RNA-seq provided evidence that intronic reads might correlate with transcriptional activity. (nature.com)
  • The beta pseudogene is composed of a BC200 RNA gene and additional Alu sequences. (wikipedia.org)
  • To validate our hypothesis , we directly compared p68 RNA sequences and the hairpin loop in silico . (lidsen.com)
  • The role of RNA interactions is crucial during complex processes such as cell differentiation in which many layers of molecular regulatory mechanism are orchestrated to promote the progression of developmental stages. (uniroma1.it)
  • The combination of different biochemical methods with computational modelling allows scientists to gain insight into molecular processes in which RNA is involved. (biosyn.com)
  • Mechanism of miR-455-3 in suppressing epithelial-mesenchymal transition and angiogenesis of non-small cell lung cancer cells. (nih.gov)
  • Moreover, exosomal miRNA-130a was transferred from CAFs to recipient NSCLC cells and knockdown of miRNA-130a reversed the effect of CAFs-derived exosomes during chemoresistance of NSCLC cells. (dovepress.com)
  • Additionally, a cluster of miRNA and piRNA was discovered on chromosome 6. (appliedbioinfo.com)
  • Both upstream and internal promoter elements are also essential for BC200 RNA synthesis. (wikipedia.org)
  • Compartmentalization of miRNAs adds complexity to the regulatory circuits of miRNA expression, thereby providing superior control over the miRNA function. (eurekaselect.com)
  • 22 candidate BmCPV-encoded miRNAs were identified in this study through small RNA sequencing, stem-loop RT-PCR and qRT-PCR. (engreen.com.cn)
  • In neuro-muscular diseases we have characterized the role of RNA interactions focusing on Rhabdomyosarcoma and the motoneuron disease Amyotrophic Lateral Sclerosis (ALS). (uniroma1.it)
  • Furthermore, the role of RNA interactions is pivotal especially in the generation of membrane-less organelles that have often crucial implications in neurodegenerative diseases such as ALS. (uniroma1.it)