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  • mRNA
  • Unsupervised clustering of samples by miRNA sequence families best reflected the clustering based on mRNA expression available for this sample set. (aacrjournals.org)
  • Although prognostic mRNA expression signatures have been defined for some invasive breast carcinomas ( 1, 2 ), the underlying pathways regulating breast cancer aggressiveness remain poorly understood. (aacrjournals.org)
  • Our meta-analysis of miRNA database revealed that 17 common miRNAs potentially interact with B7H3 mRNA. (frontiersin.org)
  • Mature miR-320 was highly expressed in brain compared with testis, whereas the inverse was observed for POLR3D mRNA, suggesting a tissue-specific role for miR-320 in regulating POLR3D expression ( Fig. 1 B ). (pnas.org)
  • By comprehensively profiling expression of miRNAs and mRNAs in serous ovarian tumors and cell lines and normal ovarian surface epithelium, we identified hundreds of potential miRNA-mRNA targeting associations underlying cancer. (aacrjournals.org)
  • Functionally, miRNAs bind to complementary sequences in the 3′ untranslated region of target gene transcripts, leading to mRNA degradation and/or translational repression ( 6 ). (aacrjournals.org)
  • Recent studies showed that ∼30% of protein levels actually correspond to mRNA levels at steady-state, suggesting that mechanisms solely regulating gene transcription or mRNA stability do not necessarily affect protein levels in cells and that changes in mRNA expression profiles frequently do not correspond to those seen in the cell's proteome ( 5 , 6 ). (jimmunol.org)
  • One mechanism by which immune cells control mRNA levels is through RNA-binding proteins (RBPs). (jimmunol.org)
  • The experimental approach included targeted transcriptional profiling of miRNA and target mRNA previously identified in rodent studies as well as genome-wide miRNA profiling using microarrays. (biomedcentral.com)
  • The biogenesis of microRNAs (miRNAs), which regulate mRNA abundance through posttranscriptional silencing, comprises multiple well-orchestrated processing steps. (pnas.org)
  • High-Throughput MicroRNA and mRNA Sequencing Reveals That MicroRNAs May Be Involved in Melatonin-Mediated Cold Tolerance in Citrullus lanatus L. (frontiersin.org)
  • Importantly, mRNA-seq analysis revealed that melatonin-induced downregulation of some miRNAs, such as miR159-5p , miR858 , miR8029-3p , and novel-m0048-3p correlated with the upregulation of target genes involved in signal transduction (CDPK, BHLH, WRKY, MYB, and DREB) and protection/detoxification (LEA and MDAR) under cold stress. (frontiersin.org)
  • analysis of miRNA
  • Clustering and comparative analysis of miRNA read frequencies showed that normal breast samples were separated from most noninvasive ductal carcinoma in situ and invasive carcinomas by increased miR-21 (the most abundant miRNA in carcinomas) and multiple decreased miRNA families (including miR-98/let-7), with most miRNA changes apparent already in the noninvasive carcinomas. (aacrjournals.org)
  • assays
  • and bEnd.3 and HeLa cells transfected with control or anti-miR-320 oligonucleotides ( D ), as measured by TaqMan miRNA assays and qRT-PCR, respectively, and normalized to GAPDH levels. (pnas.org)
  • Cell viability, invasion and wound healing assays were carried out for functional analysis after miRNA transfection. (spandidos-publications.com)
  • proteins
  • To fine-tune the production of miRNAs, plants deploy a combination of proteins that act as cofactors of the miRNA-processing machinery. (pnas.org)
  • RCF3 interacts with the phosphatases C-TERMINAL DOMAIN PHOSPHATASE-LIKE1 and 2 (CPL1 and CPL2), ultimately affecting the phosphorylation of one of the main DICER-LIKE1 (DCL1) accessory proteins, HYPONASTIC LEAVES1 (HYL1), with a concomitant effect on miRNA production. (pnas.org)
  • therapeutic
  • In addition, knocking-down specific miRNAs in these animals have demonstrated that this may consist in a promising therapeutic intervention. (frontiersin.org)
  • Scientists from the Institute of Molecular and Translational Therapeutic Strategies (IMTTS) are screening genetic material for RNAs that play a role in regulating the cardiovascular system and thereby might be putative targets for an improved treatment of cardiovascular diseases. (mh-hannover.de)
  • potentially
  • To gain insight into potentially relevant miRNAs in vivo, we investigated 2 models with differential 11β-HSD2 activity linked with salt-sensitive hypertension. (ahajournals.org)
  • distinct
  • Bioinformatic analyses showed a distinct clustering for cortical collecting ducts and proximal convoluted tubules with 53 of 375 miRNAs, where 13 were predicted to bind to the rat HSD11B2 3′-untranslated region. (ahajournals.org)
  • largely
  • miR-9-5p/3p are brain-enriched miRNAs known to regulate development and their changes have been implicated in several neurological disorders, yet their role in mature neurons in mice is largely unknown. (jneurosci.org)