Mirnas inhibit the expression of target genes regulates gene expression micro rna that regulates. Medical search. Frequent questions

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. (cancerindex.org)
The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. (cancerindex.org)
Primarily, all the cellular and molecular functions are controlled by miRNAs and as many as 60% of all human mRNAs are regulated by miRNA [6]. (rawveronica.com)
MiRNAs bind to complementary sequences in the 3-untranslated region (3-UTR) of mRNAs to regulate gene expression by inhibiting protein translation and/or causing mRNA degradation [11]. (rawveronica.com)
Due to imperfect complementary binding by miRNAs, a single miRNA can potentially bind to >100 different mRNAs [11,12]. (rawveronica.com)
MicroRNAs (miRNAs) are a category of endogenous, non-coding small molecule-RNA and play a vital role in regulating gene expression by either inhibiting the translation of target genes or causing the degradation of mRNAs. (shsmu.edu.cn)
MiRNAs bind the 3' untranslated region (3'UTR) of target mRNAs. (ukessays.com)
Mature miRNAs are generated by the RNase III enzymes Drosha and Dicer, then incorporate into the RNA-induced silencing intricate (RISC), and eventually bind to the 39-untranslated location (39-UTR) of their target gene mRNAs, inhibiting their expression[1,2]. (signsin1dayinc.com)
MiRNAs are small non-coding RNAs which regulate gene expression by binding to complementary sequences in mRNAs. (duke.edu)
Although more and animals and plants and regulate gene/protein expression more progressions have been made about the through direct complementarity between their 5' region pathogenesis of asthma in recent years, the increasing and the 3' untranslated region of target mRNAs. (cdc.gov)

MicroRNAs (miRNAs/miRs) are endogenous, single-stranded, small (approximately 22 nucleotides in length) noncoding RNA molecules that play an important and ubiquitous role in regulating genes expression. (spandidos-publications.com)
Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
Many ncRNAs have critical roles in tumorigenesis, and some ncRNAs function as competing endogenous RNAs (ceRNAs) to perturb gene expression. (biomedcentral.com)
Our findings suggest that miRNAs confer precision to protein expression and thus offer plausible explanations for the commonly observed combinatorial targeting of endogenous genes by multiple miRNAs, as well as the preferential targeting of lowly expressed genes. (knaw.nl)
MicroRNAs (miRNAs) are small endogenous RNAs that are associated with tumourigenesis, cell proliferation and apoptosis ( 4 , 5 ). (spandidos-publications.com)

Indeed, miRNAs have been shown to be actively involved in the coordination of almost all major cellular processes, such as differentiation ( 11 ), proliferation ( 12 ), metabolism, and apoptosis ( 13 ). (spandidos-publications.com)
TRIM13 inhibited cell proliferation and induced cell apoptosis by regulating NF-κB pathway in non-small-cell lung carcinoma cells. (cancerindex.org)
Studies have found that miRNA can affect a variety of biological effects, such as DNA damage repair, cell cycle arrest, cell hypoxia, proliferation and apoptosis, etc. (researchsquare.com)
It has complex functions and can inhibit cell apoptosis, promote cell transformation, participate in cell mitosis, angiogenesis, and cause tumor cells to develop drug resistance [ 7 , 8 ] . (researchsquare.com)
Down-regulation of those genes prospects to inhibition of cell proliferation, migration and invasion, and inducing apoptosis in vitro . (biotechnologyconsultinggroup.com)
As a consequence, any modulations of the target transcript expression, miRNAs can affect various signalling pathways and cellular process such as apoptosis, proliferation or differentiation. (ukessays.com)
In addition, compared to the mono-treatment, combination of Polo-like kinase 1 inhibition with anti-mitotic or DNA damaging agents boosts more severe mitotic defects, effectually triggers apoptosis and strongly inhibits proliferation of cancer cells with functional p53. (oncotarget.com)
Activation of p53 will increase miR-34a creation, and about-expression of miR-34a induces cell cycle arrest, senescence and apoptosis. (signsin1dayinc.com)
Here, I present two studies utilizing the phenotypic and the target approaches, respectively, to demonstrate that miR-BARTs can inhibit apoptosis and activate the Wnt signaling pathway. (duke.edu)
To discover miR-BARTs that can inhibit apoptosis, I individually expressed miR-BARTs in the EBV- GaCa cell line AGS, and identified five miR-BARTs that conferred this phenotype. (duke.edu)
Next, I thoroughly demonstrated that the 10 candidate target genes were substantially suppressed by expression of the relevant miR-BARTs, as measured by 3'UTR-containing firefly luciferase (FLuc) expression, mRNA and protein levels, and knockdown of seven of the 10 candidate genes could suppress apoptosis, mimicking the effects of relevant miR-BARTs. (duke.edu)
Together, my results identified and validated a substantial number of novel targets of miR-BARTs involved in apoptosis and the Wnt signaling pathway, indicating that EBV may employ miR-BARTs to heavily target these two pathways to facilitate chronic infection. (duke.edu)
The impact of JPX on Twist1 expression, cell growth, invasion, apoptosis, and in vivo tumor growth were investigated in lung cancer cells by western blotting, rescue experiments, colony formation assay, flow cytometry, and xenograft animal experiment. (biomedcentral.com)

Aptamers, which are biomaterials comprised of single-stranded DNA/RNA that form tertiary structures, have significant potential as next-generation materials, particularly for drug discovery. (biomedcentral.com)
MicroRNAs (miRNAs) are single-stranded, endogenously expressed, short, noncoding RNAs with a length of 18-25 nucleotides. (nature.com)
Through biochemical experiments in vitro, the authors directly proved that rAtRDR1 could modify single-stranded miRNA and double-stranded miRNA with 1-nt or 2-nt overhang by 3' terminal single nucleotide, but could not modify double-stranded miRNA with flat ends. (sciencemission.com)
MiRNAs are approximately 19- to 25-nucleotide estimated about 250,000 and mortality does not appear single-stranded, noncoding RNAs that exist in both to correlate well with prevalence. (cdc.gov)

By transporting "cargos" such as proteins, RNAs, DNAs, and lipids [ 26 ], exosomes regulate the eventual fate of recipient cells. (hindawi.com)
The presence of miRNAs in viruses brings forth the idea that when a viral infection penetrates a host cell the virus uses miRNAs to suppress the cells' defense systems by inhibiting the production of important proteins in the host. (thefutureofthings.com)
The blood circulating miRNA is mainly derived from a type of exosomes containing a variety of proteins and miRNAs that can be passed between cells and endocytic vesicles. (researchsquare.com)
RNA-binding proteins of the DBHS (Drosophila Behavior Human Splicing) family, NONO, SFPQ, and PSPC1 have numerous roles in genome stability and transcriptional and posttranscriptional regulation. (edu.au)
Critical to DBHS activity is their recruitment to distinct subnuclear locations, for example, paraspeckle condensates, where DBHS proteins bind to the long noncoding RNA NEAT1 in the first essential step in paraspeckle formation. (edu.au)
However, miRNAs only slightly reduce the mean expression of most targeted proteins, leading to speculation about their role in the variability, or noise, of protein expression. (knaw.nl)
Inhibition of miR‑132 activated PGC‑1α/Nrf2 signalling and inhibited oxidative stress and the expression of the pyroptosis‑related proteins NLRP3, caspase‑1 and IL‑1β, which were all reversed by inhibiting SIRT1 with EX527. (spandidos-publications.com)
Modern research confirmed that miR-22 targets various proteins such as estrogen receptor a (Period), c-Myc binding protein 11487506 (MYCBP), Myc connected element X (MAX), and PTEN, suggesting that miR-22 may possibly be implicated in tumorigenesis. (signsin1dayinc.com)
MiRNA expression is frequently altered during cancer development, associated with dysregulated expression of a plethora of different miRNAs, their biogenesis or processing proteins, such as DROSHA and DICER1 ( 1 ). (amegroups.org)

Now it is understood that they play an important role in the regulation of gene expression. (thefutureofthings.com)
demonstrates that wt renilla mRNA was expressed to similar or higher levels compared to mut suggesting that translation repression is a predominant mode of miRNA regulation. (rawveronica.com)
Given the immense potential of miRNA as a therapeutic option, these findings highlight the necessity to thoroughly examine the mode of mRNA regulation in order to achieve the beneficial effects in targeting cells. (rawveronica.com)
Post-transcriptional regulation of mRNA targets by miRNAs and their deregulation has been linked to a number of disease processes [3,7]. (rawveronica.com)
Global protein expression studies in human cells have revealed that translation repression is a predominant mode of miRNA regulation followed by the degradation of miRNA bound transcripts [13,14]. (rawveronica.com)
We hypothesize that cells may differ in their mode of miRNA regulation. (rawveronica.com)
The aim of this study is to determine if different cells lines expressing a similar miRNA target would pursue similar or alternate modes of miRNA regulation. (rawveronica.com)
We estimate that hundreds of (lowly expressed) genes in mouse embryonic stem cells have reduced noise due to substantial miRNA regulation. (knaw.nl)
This Special Issue of "Genes" seeks reviews and original papers covering a wide range of topics related to microRNA biology, such as regulation of expression in various disorders (cancer, metabolism, autoimmunity to mention but a few), genetics of microRNAs and their target sites, functional analysis of microRNA function and studies of interactions between microRNAs and target genes. (mdpi.com)
The underlying mechanism lies in that miR-20b-5p overall enhanced both CCND1 and E2F1 targets via bidirectional regulation probably involving direct downregulation and indirect upregulation. (researchsquare.com)
Previous studies have confirmed that miRNAs play an important role in gene regulation in CD44 + CD24 -/low breast cancer stem cells (BCSCs). (researchsquare.com)
Generally, the regulation of target genes by miRNAs occurs through binding with the 3′-untranslated region (UTR). (spandidos-publications.com)
1) For example, the down-regulation of miR15s and miR - 16 - 1 in chronic lymphocytic leukaemia, prostate cancer and pituitary tumours, leading to the inhibition of tumour growth and induce cell cycle arrest at the G 1 - phase by target cell cycle regulators ( cyclin D1, cyclin E1, cyclin D3 and CDK6). (ukessays.com)
MicroRNAs (miRNAs) are short non-coding RNAs that have been termed "master regulators of the genome" given their significance in post-transcriptional gene regulation, and roles in a multitude of normal and disease processes. (amegroups.org)
MicroRNAs (miRNAs) are short non-coding RNAs, typically 21-23 nucleotides long, that function in post-transcriptional gene regulation typically through translation inhibition and/or mRNA degradation ( 1 ). (amegroups.org)
As main findings, 23 miRNAs were already identified as being involved in genetic regulation of PCa cell response to RT. (frontiersin.org)
Moreover, down-regulation of let-7c expression is accompanied by up-regulation of Bcl-2 expression and down-regulation of Bax expression, both of which are the downstream genes of HSP70. (molcells.org)
MicroRNAs participate in the regulation of asthma, the goal of this study is to summarize recent researches on the roles of microRNAs in the pathogenesis of asthma. (cdc.gov)
MicroRNAs not only participate in determining DCs phenotype and then naive T lymphocyte differentiation, but also participate in the regulation of airway inflammation and airway remodeling in asthma. (cdc.gov)
As binding of miRNAs to a target mRNA may result in an airway inflammatory disease closely correlates with either mRNA degradation or inhibition of protein immune regulation, more and more evidences suggest translation.1 More than 2000 miRNAs have been discovered in humans. (cdc.gov)
Zhang et al cytokines, mediators and signals and closely related to demonstrated that microRNA let-7i was upregulated immune regulation. (cdc.gov)
DC demonstrated that miRNAs participate in airway maturation, while downregulation of let-7i significantly inflammatory regulation in asthma. (cdc.gov)

The results of these analyses revealed that the differentially expressed exosomal miRNAs participate in multiple biological processes, such as gene expression, synthesis of biomolecules, cell development, differentiation, and signal transduction, among others. (hindawi.com)
Moreover, we found that these differentially expressed exosomal miRNAs connect osteogenic differentiation to processes such as axon guidance, MAPK signaling, and Wnt signaling. (hindawi.com)
Interestingly, the results of comparative transcriptomics showed that the TPM3 gene was differentially expressed in donkey skeletal muscle S1 (2 months old) and S2 (24 months old), as verified via RT-PCR. (bvsalud.org)
Dual-luciferase test analysis showed that the TPM3 gene was targeted by differentially expressed miRNA (eca-miR-1). (bvsalud.org)
BC-related differentially expressed microRNAs (DE-miRs) were recognized and assessed in the present study. (nature.com)

MiRNAs TR-701 regulate genes involved in virtually all physiologic processes while dysregulated miRNA expression and function contributes towards TR-701 the pathogenesis of numerous diseases. (rawveronica.com)
Herein, to provide better understanding of the molecular events in GBC pathogenesis, we developed GBCdb ( http://tmliang.cn/gbc/ ), a user-friendly interface for the query and browsing of GBC-associated genes and RNA interaction networks using published multi-omics data, which also included experimentally supported data from different molecular levels. (biomedcentral.com)
A large number of studies have demonstrated that the synergistic collaboration of a number of microRNAs (miRNAs), their growth factors and their downstream agents is required for the initiation and completion of pathogenesis in the liver. (wjgnet.com)
Advances in miRNA profiling techniques have highlighted the significance of both EBV- and human genome-encoded miRNAs in the pathogenesis of NPC. (amegroups.org)
Understanding the roles of Asthma is a worldwide problem, the prevalence of miRNAs in asthma pathogenesis may also aid to asthma ranged from 1% to 18% of the population in explore new therapeutic targets. (cdc.gov)

MiRNAs typically bind to the 3′ untranslated region (UTR) of their mRNA targets and downregulate gene expression via mRNA degradation or translational inhibition ( 8 - 10 ). (spandidos-publications.com)
MicroRNAs are 18C22 nucleotides long, non-coding RNAs that hole transcripts with complementary sequences leading to either mRNA degradation or translational suppression. (rawveronica.com)
MicroRNAs (miRNAs) repress the expression of many genes in metazoans by accelerating messenger RNA degradation and inhibiting translation, thereby reducing the level of protein. (knaw.nl)

This miRNA functions as a tumor suppressor and dysregulation or loss of the host gene from which this miRNA is processed is associated with cancer progression in numerous cell types. (cancerindex.org)
Regarding the important role of microRNAs in breast cancer, investigating the molecular mechanisms of miRs and their impacts on breast cancer progression is critical. (nature.com)
According to the present data, miR-183 may play a vital role in the progression of breast cancer by reducing PTEN expression. (nature.com)
Numerous studies have found that miRNAs can be released into circular peripheral blood from tumor cells and tissues and form the circulating miRNAs with high stability and high specificity, thus serving as biomarkers for the occurrence and progression of tumors. (shsmu.edu.cn)
The circulating miRNA expressions in patients with endometrial cancer are different from those of healthy people and change specifically with the progression of tumors. (shsmu.edu.cn)
Abnormal changes in miRNAs have been discovered in breast cancer, and miRNAs have been demonstrated to be associated with the tumorigenesis and progression of breast cancer [8, 10]. (researchsquare.com)
Lastly, RDR1 inhibits the progression of various mouse solid tumors and leukemia. (sciencemission.com)
In cancer, dysregulation of miRNAs can facilitate disease progression and therapeutic resistance, affecting tumour-suppressors and oncogenes. (amegroups.org)
These miRNA alterations are potential diagnostic and prognostic biomarkers when assessing patient disease progression or guiding clinical management. (amegroups.org)

MicroRNAs are small, non-coding RNA molecules, which target genes and regulate gene expression and function. (mesotheliomagroup.com)
These indicate that miR-133b play a role as a tumor suppressor gene in osteosarcoma through inhibiting PI3K/Akt signaling and down-regulating several anti-apoptotic molecules and oncogenes such as BCL2T2, MCL-1, IGF1R, MET, and FAK. (biotechnologyconsultinggroup.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Small non-protein-coding RNA molecules, composed of around 22 nucleotides, are commonly named as miRNAs ( 1 - 3 ). (frontiersin.org)

Gene mutations and aberrant signaling pathways play key roles in GBC tumorigenesis. (biomedcentral.com)
MicroRNAs (miRNAs) decrease the reflection of particular focus on oncogenes or growth suppressor genetics and thereby play crucial assignments in tumorigenesis and growth development. (biotechnologyconsultinggroup.com)
MiRNAs have a range of physiological and pathological capabilities, such as regulate of tumorigenesis[10,11,12]. (signsin1dayinc.com)
The study reveals for the first time that abnormal 3' terminal short 1-nt miRNA isomers are widely accumulated in various human primary tumors, which provides a new insight into the reduction of global miRNA dose during tumorigenesis. (sciencemission.com)

In turn, one gene can be simultaneously regulated by multiple miRNAs [13]. (rawveronica.com)
Genes that are regulated by multiple miRNAs show more-pronounced noise reduction. (knaw.nl)

This study confirms that alterations in the expression of exosomal miRNAs can promote osteogenic differentiation of ADSCs, which also provides the foundation for further research on the regulatory functions of exosomal miRNAs in the context of ADSC osteogenesis. (hindawi.com)
Most of these paracrine secretions include soluble factors and exosomes, which regulate the repair and regeneration processes at sites of damage by affecting cell proliferation, migration, and differentiation [ 22 , 23 ]. (hindawi.com)
MicroRNAs constitute a pervasive post-transcriptional filter on protein or mRNA expression levels that are likely to control developmental timing, cellular differentiation, stress responses, metabolism and proliferation. (mdpi.com)
However, the role of microRNA in mammalian tissue development and differentiation is still quite uncharacterized. (mdpi.com)
Because of their unique expression profile and special functions, miRNAs have been used as molecular markers to define the direction and processes of cell differentiation by regulating one or several target genes. (researchsquare.com)

So, miRNAs may influence many cellular activities. (spandidos-publications.com)
Single-cell (SC) gene expression analysis is crucial to dissect the complex cellular heterogeneity of solid tumors, which is one of the main obstacles for the development of effective cancer treatments. (biomedcentral.com)
It was revealed that cellular oncogenicity is positively regulated by miR-183 by inhibiting the expression of PTEN. (nature.com)
Therefore, it is important to describe their mechanisms of actions, expression patterns and possible target genes and cellular pathways with which they interact. (mdpi.com)
Sirtuin 1 (SIRT1), a member of the sirtuin family, regulates the cellular ageing process, participates in metabolic diseases, such as diabetes, and is related to cancer development ( 13 ). (spandidos-publications.com)
This review integrates the literature on EBV-miRNAs, cellular miRNAs, and miRNA signatures to guide future research, while acknowledging challenges in the selection and clinical implementation of relevant miRNA biomarkers and signatures. (amegroups.org)

On the other hand, in order to identify miR-BARTs that can activate the Wnt signaling pathway, I analyzed the PAR-CLIP data set of C666 cells and discovered nine anti-Wnt signaling targets of miR-BARTs, including seven novel genes and two pro-apoptotic genes identified above. (duke.edu)
Utilizing a Wnt signaling FLuc reporter TOPflash which measures the Wnt signaling activation, I confirmed that expression of many miR-BARTs that target Wnt signaling inhibitors can indeed upregulate the Wnt signaling pathway. (duke.edu)
Therefore, it would be a new strategy for tumor treatment to inhibit tumor cell proliferation by repairing the defective miRNA pathway in tumors. (sciencemission.com)
RDR1, as ectopically expressed plant immune protein, modifies these double stranded free miRNA isomers of AGO2 through its single nucleotide tailing, to reactivate the defective miRNA pathway and specifically block the cycle of cancer cells in solid tumors and leukemia. (sciencemission.com)
The authors proposed that plant RDR1 can inhibit cell cycle and proliferation by increasing global miRNA expression to specifically recover miRNA deficiency in cancer cells through knockdown and AGO2-CLIP of key components of miRNA pathway. (sciencemission.com)
The authors also proved that RDR1 with nucleotide transferase activity can modify the short 1-nt double-stranded miRNA isomers isolated from AGO2 by single nucleotide, so as to restore their loading efficiency to AGO2, and finally repair the defective miRNA pathway in cancer. (sciencemission.com)
Thus, the elucidation of a new oncogenic pathway is required to precisely target lung cancer and to serve as a prognostic factor. (biomedcentral.com)

Ago-2 immunoprecipitation show that miRNA repressed renilla mRNA are associated with functional mi-RISC TR-701 (miRNA-RNA induced silencing complex). (rawveronica.com)
One of the strand of duplex RNA (guide strand) directs RNA-induced silencing complex (RISC) to exert repressive function on its target mRNA [8,10]. (rawveronica.com)
Mammalian mature miRNA double stranded with 2-nt 3' terminal overhang can be recognized and loaded by Argonaute (AGO) to form RNA Induced Silencing Complex (RISC) to regulate the expression of target genes. (sciencemission.com)

The research group published a research paper in Cell , which introduces that miRNA isomers that cannot effectively bind to the 1-nt-shorter 3′ends of the AGO2 complex are widely accumulated in different samples of human primary cancers and cancer-cell lines. (sciencemission.com)

These TR-701 miRNAs regulate various biological systems in mammals including developmental and physiological networks [3]. (rawveronica.com)
This makes miRNA a biological factor predicting NSCLC patients [ 4 ] . (researchsquare.com)
In 2009, miRNA were first suggested to have biological roles in MPM by Guled and colleagues. (mesotheliomagroup.com)
GBCdb will help to elucidate the potential biological roles of different RNAs and allow for the exploration of RNA interactions in GBC. (biomedcentral.com)
Recent studies, including our previous findings, have demonstrated that miRNAs play an important role in regulating biological functions [6-8]. (researchsquare.com)
In recent years, many studies have confirmed the involvement of miRNAs in biological processes of several types of cancer ( 4 , 9 , 10 ). (frontiersin.org)

This algorithm, named RepTar runs a search on the known genes in the human genome and detects matches to the various miRNAs by taking into account sequence, structural, and physical considerations. (thefutureofthings.com)
C-myc decreases the expression of various miRNAs like miR-22 in most cancers cell lines[thirteen]. (signsin1dayinc.com)

The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. (cancerindex.org)

Firstly, RDR1 protein inhibits the proliferation of cancer cells by targeting cell cycle. (sciencemission.com)

The MMP7 native miRNAs called the primary-miRNAs (pri-miRNA) are situated in the nucleus where they are processed by the Drosha and DiGeorge syndrome critical region 8 (DGCR8) complex into precursor-miRNA (pre-miRNA). (rawveronica.com)

The findings of the present study indicated that inhibition of miR‑132 may ameliorate myocardial I/R injury by inhibiting oxidative stress and pyroptosis through activation of PGC‑1α/Nrf2 signalling by targeting SIRT1. (spandidos-publications.com)

To achieve this, p53 mainly functions as a transcription factor (TF) targeting hundreds of genes, but it also has TF-independent roles in the nucleus and cytoplasm [ 6 ]. (nature.com)
The results of the present study indicate that microRNAs play important roles in regulating asthma immune responses. (cdc.gov)

It is believed that consecutive foundation pairing of at the very least seven nucleotides among the miRNA sequence (seed sequence) and the miRNA recognition element (MRE) is necessary to repress protein translation[three,4,5,6,seven]. (signsin1dayinc.com)

In their research, the Guled team explained that "miRNA were predicted to target some of the more commonly affected genes in MPM including cyclin-dependent kinase inhibitor 2A (CDNK2A), neurofibromatosis type 2 (NF2), jun oncogene, hepatocyte growth factor and platelet derived growth factor. (mesotheliomagroup.com)

MicroRNAs (miRNAs) and Twist1-induced epithelial-mesenchymal transition (EMT) in cancer cell dissemination are well established, but the involvement of long noncoding RNAs (lncRNAs) in Twist1-mediated signaling remains largely unknown. (biomedcentral.com)

His study showed that a viral miRNA inhibits the activity of the abovementioned gene in human immune system cells. (thefutureofthings.com)
When a microRNA is present in the cell, it binds to the target sites and downregulates GFP expression, while in cells that do not express the microRNA GFP, it is expressed. (lu.se)
The miRNA profile between breast cancer stem cells(BCSCs, CD44+CD24-/low) and control MCF-7 breast cancer cells was obtained in a previous study. (researchsquare.com)
MiRNAs can also control the self-renewal or proliferation of stem cells [9]. (researchsquare.com)
In our previous research, we performed miRNA profiling between sorted CD44 + CD24 -/low BCSCs and the control MCF-7 breast cancer cells [8]. (researchsquare.com)
Their expression in tumour cells is modulated by a complex interplay of genomic, transcriptomic and post translational factors involving multiple intracellular antigen processing pathways. (portlandpress.com)
Ongoing research investigates mechanisms invoked by cancer cells to abrogate MHC-I expression and attenuate anti-tumour CD8 + cytotoxic T cell response. (portlandpress.com)
This review provides a comprehensive overview of physiologically conserved or novel mechanisms utilised by tumour cells to reduce MHC-I or MHC-II expression. (portlandpress.com)
A) and (C) Steady over-expression of miR-133b in U2-Operating-system and MG-63 cells. (biotechnologyconsultinggroup.com)
Essential contraindications reflection of miR-133b in osteosarcoma cells U2-Operating-system and MG-63 was examined by qRT-PCR Tofacitinib citrate with total RNAs singled out from the indicated cells (d? (biotechnologyconsultinggroup.com)
U2-Operating-system or MG-63 cells had been transfected with miR-133a imitate or miRNA imitate detrimental control (NC) at a last focus of 50 nM. (biotechnologyconsultinggroup.com)
Cells had been farmed after 48 hours and total RNAs had been removed. (biotechnologyconsultinggroup.com)
A) Cells were transfected with miR-133a mimic or miRNA mimic bad control (NC) at a final concentration of 50 nM. (biotechnologyconsultinggroup.com)
p53 represses the promoter of Polo-like kinase 1, whereas Polo-like kinase 1 inhibits p53 and its family members p63 and p73 in cancer cells lacking functional p53. (oncotarget.com)
Plk1 inhibitors target all rapidly dividing cells irrespective of tumor cells or non-transformed normal but proliferating cells. (oncotarget.com)
In EBV latently infected epithelial cells, such as nasopharyngeal carcinoma (NPC) and gastric carcinoma (GaCa) cells, viral protein expression is low. (duke.edu)
The miRNAs are small stretches of RNA made by host cells to regulate gene expression. (feedstuffs.com)
The authors cloned RDR1 gene respectively from Arabidopsis (At) and rice (Os) into lentivirus vector induced by Dox, and verified its successful ectopic expression in mammalian cells. (sciencemission.com)
At the molecular level, Gene Set Enrichment Analysis (GSEA) based on RNA-seq showed that RDR1 in At and Os could interfere with cycle processes in all cancer cell lines, while it had no significant effect in non-cancer control cells. (sciencemission.com)
The authors believe that RDR1 is an exogenous tumor suppressor which can specifically target and interfere with the cycle process in cancer cells without influence on non-cancer cells. (sciencemission.com)
And through systematic analysis on the published miRNA sequencing data and the miRNA sequencing of AGO2-IP, the authors suggest that it is not so effective and stable for the abnormal short 1-nt double-stranded miRNA isomer to enter into AGO2 in cancer cells, so it may be related to the reduction of miRNA dose in different tumors. (sciencemission.com)
These findings suggest that lncRNA JPX, a mediator of Twist1 signaling, could predispose lung cancer cells to metastasis and may serve as a potential target for targeted therapy. (biomedcentral.com)
The main goal of RT consists in delivering a precise dose of radiation in a target volume, such as tumor, promoting the tumor cells eradication with as minimal damage as possible in surrounding normal tissues ( 13 ). (frontiersin.org)
Th)1 cells.5 Meanwhile, the function and immunity of with genetic predisposition, involving multiple cells, DCs are closely regulated by miRNAs. (cdc.gov)

In a previous study using a microRNA (miRNA/miR) microarray assay, we demonstrated that miR-133b-5p was upregulated in response to hypoxic preconditioning (HPC). (spandidos-publications.com)
To confirm the results of miRNA microarray assay, SYBR Green qRT-PCR was performed using the RNAs from five human osteosarcoma and three normal muscle mass samples in miRNA microarray assay as themes. (biotechnologyconsultinggroup.com)

The biogenesis and mode of action of miRNA have been well characterized [8,9]. (rawveronica.com)
Here, we aim to provide an overview on the mechanisms of miRNA biogenesis and to discuss the functional impact of miRNAs on PCa under radiation response. (frontiersin.org)

It would also enable scientists to create synthetic miRNAs that would mimic the action of the natural ones suppressing the immune system in autoimmune diseases and transplantations. (thefutureofthings.com)
We also observed that miRNA or control miRNA mimic-transfected DC show remarkably altered mRNA expression of various genes examined while, only few were impacted in M [16]. (rawveronica.com)
Exiqon) with the miRCURY LNATM miRNA Array Electricity one hundred pmol miRNA mimic in accordance to the manufacturer's guidelines. (signsin1dayinc.com)

Approximately 30-60 % of the human genome, regulated by miRNAs. (ukessays.com)
Briefly, miRNAs are expected to account for 1-5% of the human genome and to interfere with at least 30% of the protein-coding genes ( 4 , 5 ). (frontiersin.org)

The binding of miRNA- protein complexes to mRNA causes translation inhibition or destabilisation of target transcripts. (ukessays.com)

miR‑132 directly targeted SIRT1 and negatively regulated the expression of SIRT1. (spandidos-publications.com)

Therefore, as novel biomarkers, circulating miRNAs have broad application prospects for the early diagnosis and prognostic evaluation of endometrial cancer. (shsmu.edu.cn)
Research progresses in circulating microRNAs as novel biomarkers for endometrial cancer[J]. , doi: 10.3969/j.issn.1674-8115.2016.05.030 . (shsmu.edu.cn)
Explains the HIPMR team, "miRNA can function as oncogenes and/or tumor suppressors and they also have characteristics that make them "attractive biomarkers" including being stably expressed in fluid and tissue and "easily measurable using techniques such as quantitative real-time PCR (qPCR). (mesotheliomagroup.com)
Identification of miRNAs panel can be thus considered an upcoming and potentially useful strategy in PCa diagnosis, given that radioresistance biomarkers, in both prognosis and therapy still remains a challenge. (frontiersin.org)

Scope includes mutations and abnormal protein expression. (cancerindex.org)
Abnormal expression of miRNA plays a relevant role in cancer biology and they are therefore a potential target for the development of innovative cures," notes SHRO. (mesotheliomagroup.com)

The mechanisms of radioresistance are still poorly understood, despite it has been suggested that miRNAs play an important role in cell signaling pathways. (frontiersin.org)

Splicing factor proline- and glutamine-rich (SFPQ) is a nuclear RNA-binding protein that is involved in a wide range of physiological processes including neuronal development and homeostasis. (edu.au)

Moreover, it was revealed that miRNAs can act as tumor suppressors or oncogenes. (nature.com)
1) The alternation of protein levels of critical oncogenes or tumour suppressor genes, which causes by miRNAs deregulation may also lead to proliferative diseases such as cancer. (ukessays.com)
These are divided into two group, those miRNAs that are upregulated in cancer which are likely to be acting as oncogenes and those downregulated in cancer which are likely to be acting as tumour suppressors. (ukessays.com)
The first miRNA was discovered in 1993 by Lee, Freinbaum and Ambros ( 6 , 7 ), and since then an increasing load of literature data have pointed that they can act as both tumor suppressors and oncogenes ( 1 - 3 ). (frontiersin.org)

The algorithm predicted that an immune system protein would be inhibited by the viral miRNA. (thefutureofthings.com)
It may open a new direction for anti-viral therapy aiming to inhibit viral miRNA and thus stopping a virus from reproducing. (thefutureofthings.com)
Another possible direction of research is suppressing specific genes involved in tumor development by using synthetic or viral miRNA. (thefutureofthings.com)
In contrast, a cluster of viral microRNAs (miRNAs) called miR-BARTs is highly expressed. (duke.edu)
Now, the same team - including researchers in the laboratories of Charles M. Rice, the Maurice R. & Corinne P. Greenberg professor and head of the Laboratory of Virology & Infectious Disease, and Robert B. Darnell, the Robert & Harriet Heilbrunn professor and head of the Laboratory of Molecular Neuro-oncology - has found evidence that another RNA virus - bovine viral diarrhea (BVD) virus, which infects cows and other livestock - also depends on miRNA for infection. (feedstuffs.com)

Recent mammalian cell line studies have demonstrated that some miRNAs participate in fine-tuning the production of their targets, both at the mRNA as well as the protein level and play an important role as genetic expression regulators [13,14]. (rawveronica.com)
During their search for new diagnostic and therapeutic targets for mesothelioma, researchers at Harry Perkins Institute of Medical Research (HPIMR) say "the small but powerful gene regulators microRNA (miRNA) have become of interest," in the diagnosis and treatment of malignant pleural mesothelioma (MPM). (mesotheliomagroup.com)
Non-coding RNAs (ncRNAs), mainly including microRNAs (miRNAs), long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs), can function as important regulators in gene expression. (biomedcentral.com)
MicroRNAs control the levels of numerous cell cycle regulators that controls cell proliferation. (ukessays.com)
Since their discovery in Caenorhabditis elegans , miRNAs have been extensively investigated as master regulators of gene expression in a variety of animal, plant, and human models. (amegroups.org)

p53's ability to inhibit tumors can be considered as a specific outcome of its stress-responsive effects. (nature.com)
Polo-like kinase 1, a pivotal regulator of mitosis and cytokinesis, is highly expressed in a broad spectrum of tumors and its expression correlates often with poor prognosis, suggesting its potential as a therapeutic target. (oncotarget.com)
Secondly, 3'- terminal short 1-nt miRNA isomers are widely accumulated in a variety of tumors. (sciencemission.com)

As miRNAs proposed to control the expression up to one third of all genes and possibly utilised as diagnostic and prognostic marker for many genetic based diseases. (ukessays.com)

However, the effect and molecular mechanism of miRNAs and target genes on bovine immature SC remains poorly understood. (bvsalud.org)
Therefore, to improve treatment efficacy, it is necessary to tailor therapies to patient and tumor characteristics, using appropriate molecular targets. (frontiersin.org)
The survivin gene coding product consists of 142 amino acids and has a molecular weight of 16.2 KD. (researchsquare.com)

The transcription element most commonly mutated in cancer, p53, regulates a set of miRNAs. (signsin1dayinc.com)

MiRNAs are 18C22 nucleotides (nts) long, non-coding RNAs that post-transcriptionally regulate mRNA stability and control the translation efficiency of their target genes [4,5]. (rawveronica.com)

We found that TRIM13 mRNA and protein expression was reduced in NSCLC tissues and cell lines in comparison to paired non-cancerous tissues and a human normal bronchial epithelial cell line, respectively. (cancerindex.org)
It has been proven that the miR-183 expression is up-regulated in tumor tissues and patient serum in BC, which indicates its possibly an oncogenic role 15 , 16 . (nature.com)
RT-qPCR and western blotting were conducted to detect the expression levels of lncRNA JPX and Twist1 in lung cancer cell lines and tissues. (biomedcentral.com)

Micro RNAs (miRNA's) are a type of small gene that were first discovered in 1993 and were considered unimportant for a long time. (thefutureofthings.com)

Currently there exist gaps in our understanding of how the posttranscriptional mechanism of gene expression works and how deregulated miRNA expression can link to the etiology of numerous diseases including cancer. (rawveronica.com)
We should be on guard against patients with APT complicated by lung cancer, when miR-29a was low expression in serum and survivin was high expression in diseased tissue of the patient who was pathologically diagnosed with APT. (researchsquare.com)
We selected miR-183 for study among the considerably dys-regulated microRNAs in breast cancer. (nature.com)
An effort is already underway in the lung cancer field with researchers "performing a series of tests to optimize miRNA quantification in serum from a cohort of more than 1000 patients," notes the HPIMR team. (mesotheliomagroup.com)
Our findings provide a platform to improve understanding of the detailed multi-omics RNA landscapes of GBC, especially for GBC-associated RNA interactions, which will support future studies on cancer treatment. (biomedcentral.com)
MicroRNAs (miRNAs) have become a hot topic in cancer research, but the underlying mechanism of its involvement in cancer remains unclear. (researchsquare.com)
Therefore, miRNAs could consider as cancer targets. (ukessays.com)
Besides cancer, some miRNAs genes cause or contribute in many inherited and genetic based diseases. (ukessays.com)
The understanding of miRNA has increased over the past 10 years, and particularly the involvement of miRNAs in cancer. (ukessays.com)
A greater understanding of miRNA could possibly lead to better diagnostic and treatment of cancer. (ukessays.com)
These 12 genes are located at a region, which is frequently deleted in cancer. (ukessays.com)
Recently, the research group discovered that a plant immune protein enables broad anti-tumor response by alleviating micro-RNA deficiency, which provides a powerful weapon against cancer. (sciencemission.com)
In fact, dose reduction of global miRNA is considered to be one of the causes of cancer. (sciencemission.com)
Thirdly, RDR1 repairs miRNA isomers in cancer by single nucleotide tailing. (sciencemission.com)
These clinical evaluations demonstrate the importance and potential of further miRNA research for clinical use, especially in the diagnosis and treatment of cancer. (amegroups.org)
The relationship between miRNAs and cancer was demonstrated for the first time in 2002, with miRNAs being stated as a potential mechanism that may contribute to improve some cancer therapeutic approaches through restoring or blocking the miRNAs function ( 11 ). (frontiersin.org)

The present study explored the expression and potential role of TRIM13 in non-small-cell lung carcinoma (NSCLC). (cancerindex.org)
microRNA (miRNA) is a small molecule RNA that can be stably expressed in body fluids such as serum, plasma, saliva and can be sensitively detected. (researchsquare.com)
The problem with bioinformatic searches for miRNA targets is that they are small enough to occur every few-thousand bases in the transcriptome,' said study first author Troels Scheel, now at the University of Copenhagen in Denmark. (feedstuffs.com)

These miRNAs have been implicated in critical NPC processes such as epithelial-to-mesenchymal transition (EMT) and metastasis, while further promoting chemoresistance and radioresistance. (amegroups.org)

in 1993 [1], over 2500 miRNAs controlling the mammalian genome have been discovered [2]. (rawveronica.com)
Since the first microRNA was identified in 1993 by Victor Ambros and colleagues (the lin-4 heterochronic gene in C. Elegans ), more than 24,000 microRNAs have now been identified. (mdpi.com)

Similarly, miRNA-based therapeutics are also in development for the treatment of a variety of human diseases. (amegroups.org)

They are RNA sequences transcribed from DNA but not translated into protein and are first created as a long strand and then diced in the cell nucleus by special enzymes into tiny strands. (thefutureofthings.com)
TFOT has previously covered a discovery made at UCSD, California, of a DNA sequence that turns on and off gene expression in a cell. (thefutureofthings.com)
To a great extent, the identities of these cell types are determined by their patterns of gene expression. (rawveronica.com)
Our study also explored the potential for maneuvering miRNA levels to modify gene expression in a cell-specific manner. (rawveronica.com)
A number of miRNA have now been identified as aberrantly expressed in MPM with a select few shown to regulate cell activity. (mesotheliomagroup.com)
The lncRNA TMPO-AS1 promotes cell proliferation, migration, invasion and epithelial-to-mesenchymal transition by regulating the miR-1179/E2F2 axis [ 13 ]. (biomedcentral.com)
We used mathematical modeling and single-cell reporter assays to show that miRNAs, in conjunction with increased transcription, decrease protein expression noise for lowly expressed genes but increase noise for highly expressed genes. (knaw.nl)
Over-expression of miR-133b in osteosarcoma cell lines U2-OS and MG-63 decreases the manifestation of BCL2T2, MCL-1, IGF1R, MET and FAK, leading to the inactivation of Akt. (biotechnologyconsultinggroup.com)
Over-expression of miR-133b in osteosarcoma cell lines U2-Operating-system and MG-63. (biotechnologyconsultinggroup.com)
To identify pro-apoptotic genes targeted by the five anti-apoptotic miRNAs, I validated one previously published target and identified nine novel targets by performing photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP) in the EBV+ NPC cell line C666. (duke.edu)
Moreover, computational approaches typically don't know what's expressed in different cell types,' and therefore, which mRNA sequences they identify as miRNA targets might be physiologically relevant. (feedstuffs.com)
Surprisingly, many miRNAs can directly target and inhibit cell cycle genes to control cell proliferation. (sciencemission.com)
Indeed, it has been shown that miRNAs play an important role in gene expression, mainly when associated with the monitoring of several cell and metabolic pathways, being also an essential component of the gene silencing machinery in most eukaryotic organisms ( 4 , 8 ). (frontiersin.org)
Wound healing is a complex physiological process necessitating the coordinated action of various cell types, signals and microRNAs (miRNAs). (molcells.org)

PTEN was validated by dual luciferase assay as a target gene of miR-183. (nature.com)
The target genes were predicted by the online programs picTar, miRanda and TargetScan and verified by dual luciferase assay, and changes in protein expression were detected by western blot. (researchsquare.com)
MiR-20b-5p directly targeted both CCND1 and E2F1 in a dual luciferase assay, while antagomir-20b-5p downregulated the protein levels of CCND1 and E2F1. (researchsquare.com)

In addition, some studies recommend that imperfect binding such as wobbles or bulges in the seed sequence inhibits protein translation [eight,9]. (signsin1dayinc.com)

The non-sense miRNA Pre-miR miRNA Precursor Adverse Control #one (Daily life systems) was utilised as a regulate for specificity of interaction. (signsin1dayinc.com)
Scientists could weaken replication and production of BVD virus by inhibiting its interaction with micro-RNA. (feedstuffs.com)

The survivin gene is 15 KB in length, located at 17q25, and has 4 exons and 3 introns. (researchsquare.com)

Anatomy10

Organisms10

Chemicals and Drugs55

Analytical, Diagnostic and Therapeutic Techniques and Equipment23

Phenomena and Processes66

Disciplines and Occupations3

Information Science5

Health Care2

MRNAs10

Endogenous5

Apoptosis13

Single-stranded4

Proteins9

Regulation22

Differentially expressed5

Pathogenesis5

Degradation3

Progression9

Molecules4

Tumorigenesis4

Multiple miRNAs2

Differentiation5

Cellular6

Pathway7

RISC3

Bind1

Biological6

Various miRNAs2

Mature miRNA1

Proliferation of cancer cells1

Precursor miRNA1

Oxidative stress1

Roles2

Repress1

Cyclin-dependent kinase1

Epithelial-Mesenchyma1

Cells24

Microarray2

Biogenesis2

Mimic3

Human genome2

Inhibition1

Negatively regulated1

Biomarkers4

Abnormal2

Mechanisms1

Range of physiological1

Oncogenes4

Viral5

Regulators5

Tumors3

Diagnostic and prognostic1

Molecular3

Commonly1

Stability1

Tissues3

Discovered in 19931

Cancer16

Small3

Metastasis1

19932

Therapeutics1

Cell14

Assay3

Protein translation1

Interaction2

Survivin1