• miRNAs are thought to have regulatory roles through complementarity to mRNA. (wikipedia.org)
  • It plays an important role in controlling mRNA expression. (wikipedia.org)
  • Bioinformatic predictions suggest that the human EGFR mRNA 3'-untranslated region contains three microRNA-7 (miR-7) target sites, which are not conserved across mammals. (wikipedia.org)
  • MicroRNAs (miRNAs) are small noncoding endogenous RNAs containing 19 to 25 nucleotides that promote posttranscriptional control in regulating the expression of target gene by binding to the 3′-UTR sequences of its mRNA [ 5 ]. (hindawi.com)
  • MicroRNAs (miRNAs), a class of small, endogenous, non-coding RNAs, are able to regulate gene expression by translational repression or mRNA degradation of the target, thereby affecting critical functions in various physiological processes, ranging from cell proliferation to apoptosis ( 8 , 9 ). (iiarjournals.org)
  • miRNAs exert their function via base pairing with complementary mRNA molecules, resulting in gene silencing via transcriptional repression or target degradation. (biogenex.com)
  • We show that, similar to metazoan steady-state systems, endogenous miRNAs in Chlamydomonas can regulate gene expression both by destabilization of the mRNA and by translational repression. (freethoughtblogs.com)
  • The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. (cancerindex.org)
  • A possible model for AP2 regulation by miRNA172 can be envisioned in which expression of miRNA172 in the inner two floral whorls causes' translational inhibition of AP2 mRNA in these whorls. (oboolo.com)
  • The mRNA expression of diagnostic genes was determined by qRT-PCR analysis. (nature.com)
  • We found that TRIM13 mRNA and protein expression was reduced in NSCLC tissues and cell lines in comparison to paired non-cancerous tissues and a human normal bronchial epithelial cell line, respectively. (cancerindex.org)
  • microRNAs (miRNAs) are small non-coding RNAs that regulate gene expression by either blocking translation or inducing degradation of target mRNA. (shengsci.com)
  • MicroRNAs (miRNAs) are short non-coding RNAs that regulate gene expression via mRNA targeting, playing important roles in the pancreatic islets. (lu.se)
  • ADAR editing in premRNAs recodes open reading frames and alters splicing, mRNA structure and interactions with miRNAs. (muni.cz)
  • Multiple linear regression analysis was used to construct the model and find the significant mRNA-miRNA associations. (edu.hk)
  • We identified three significantly associated mRNA-miRNA pairs: BCL2 was positively associated with miR-16 and SMAD4 was positively associated with miR-567 in the CRC tissue, while MSH6 was positively associated with miR-142-5p in the normal tissue. (edu.hk)
  • MicroRNAs (miRNAs) are short non-coding RNAs, typically 21-23 nucleotides long, that function in post-transcriptional gene regulation typically through translation inhibition and/or mRNA degradation ( 1 ). (amegroups.org)
  • Aberrant changes in epigenetic mechanisms regulating DNA methylation, histone methylation and acetylation, expression of noncoding RNAs, and mRNA methylation are associated with the initiation, growth, and progression of digestive system tumors [ 2 , 4 ]. (ijbs.com)
  • The miRNA-mRNA pairing triggers casein kinase 1α (CK1α)-induced phosphorylation and dissociation of AGO2 from the active complex, whereas the ANKRD52-PPP6C phosphatase complex dephosphorylates AGO2 to restore its miRNA loading activity 21 , 22 . (nature.com)
  • MicroRNAs (miRNAs) are endogenous small noncoding RNAs that negatively regulate the expression of target genes through mRNA cleavage, translational repression and DNA methylation. (plantae.org)
  • Incorporated into one member of the Argonaute (AGO) protein family in the RNA induced silencing complex (RISC), a mature miRNA binds typically to the 3' untranslated region (3' UTR) of the targeted messenger RNA (mRNA) and inhibits its translation via various mechanisms. (mirtar2go.org)
  • The key determinant of the target recognition is a short sequence homology between the miRNA seed sequence (the 2nd-7th nucleotides of the miRNAs) and the targeted mRNA. (mirtar2go.org)
  • To this end we developed miRTar2GO, a method that predicts miRNA targets by allocating CLIPed regions of the mRNA 3' UTRs to miRNA seed regions. (mirtar2go.org)
  • As binding of miRNAs to a target mRNA may result in an airway inflammatory disease closely correlates with either mRNA degradation or inhibition of protein immune regulation, more and more evidences suggest translation.1 More than 2000 miRNAs have been discovered in humans. (cdc.gov)
  • Micro RNAs (MiRNAs) are endogenous, small RNA molecules that were identified in a wide range of eukaryotes. (usda.gov)
  • MicroRNA (miRNA) is a short and endogenous RNA molecule that regulates posttranscriptional gene expression. (edu.hk)
  • Circular RNAs (circRNAs) are a new type of endogenous noncoding RNA that exhibit a variety of biological functions. (biomedcentral.com)
  • Multiple roles and targets of miR-7 as well as its expression pattern were linked to regulatory mechanisms and pathogenesis in glioblastoma, breast cancer and other types of cancers, as well as in schizophrenia and visual abnormalities. (wikipedia.org)
  • Recently, increasing evidences elucidated that miRNAs was involved in the cancer pathogenesis, including cell proliferation, metastasis, and apoptosis [ 6 ]. (hindawi.com)
  • Oxidative stress and neuroinflammation play important roles in the pathogenesis of AD. (nature.com)
  • Our study identified 9 key genes associated with oxidative stress and immune reaction in AD pathogenesis. (nature.com)
  • Hepatic Stellate Cells and microRNAs in Pathogenesis of Liver Fibrosis. (shengsci.com)
  • The miRNA-driven network-based approach presented in this study contributes to a better understanding of impaired insulin secretion in T2D pathogenesis. (lu.se)
  • The malaria parasite exerted a potent selective signature on the human genome, which is apparent in the genetic polymorphism landscape of genes related to pathogenesis. (biomedcentral.com)
  • Advances in miRNA profiling techniques have highlighted the significance of both EBV- and human genome-encoded miRNAs in the pathogenesis of NPC. (amegroups.org)
  • MicroRNAs participate in the regulation of asthma, the goal of this study is to summarize recent researches on the roles of microRNAs in the pathogenesis of asthma. (cdc.gov)
  • Understanding the roles of Asthma is a worldwide problem, the prevalence of miRNAs in asthma pathogenesis may also aid to asthma ranged from 1% to 18% of the population in explore new therapeutic targets. (cdc.gov)
  • Although more and animals and plants and regulate gene/protein expression more progressions have been made about the through direct complementarity between their 5' region pathogenesis of asthma in recent years, the increasing and the 3' untranslated region of target mRNAs. (cdc.gov)
  • Most eukaryotic organisms possess highly conserved RNA silencing machinery that generates 21- ~ 24-nucleotide small RNAs from double stranded RNA precursors. (ttu.edu)
  • Background: MicroRNAs (miRNA) are small, highly conserved, non-coding RNAs that regulate gene expression of target mRNAs through cleavage or translational inhibition. (usda.gov)
  • This miRNA is a member of the highly conserved miR-34 family. (cancerindex.org)
  • Exosomes are found in abundance in endosome-derived components, which are considered to play important roles in intercellular communication due to their ability to transfer "cargos" [ 24 , 25 ]. (hindawi.com)
  • These small RNAs, which include microRNA (miRNA) and several classes of small interfering RNAs (siRNA), play important roles in regulating gene expression, heterochromatin formation, and defense against invading viruses. (ttu.edu)
  • Background: As microRNAs play important roles in cancer development and progression by regulating the expressions of oncogenes and tumor suppressor genes though interacting with the 3' untranslated region (UTR) of target genes, we aimed to evaluate the association between genetic variants of miRNAs and their binding sites and prognosis in patients with colorectal cancer (CRC). (iiarjournals.org)
  • MicroRNAs (miRNAs) are small non-coding RNAs of approximately 22 nucleotides in length, which play important roles in regulating gene expression post-transcriptionally. (geneticsmr.org)
  • Parasite and human genetic factors play important roles in malaria susceptibility and disease severity. (biomedcentral.com)
  • Proton pumps and pH regulation are known to play important roles in apoptosis signaling [15,16]. (biomedres.us)
  • The results of the present study indicate that microRNAs play important roles in regulating asthma immune responses. (cdc.gov)
  • By transporting "cargos" such as proteins, RNAs, DNAs, and lipids [ 26 ], exosomes regulate the eventual fate of recipient cells. (hindawi.com)
  • MicroRNAs (miRNAs) are short (21-24 nucleotides) sequences of RNA that reduce gene expression by promoting the breakdown of messenger RNAs (mRNAs) and by repressing translation of mRNAs into proteins. (freethoughtblogs.com)
  • Maintenance of class B gene expression The maintenance of AP3 and PI expression in flowers requires the activity of both AP3 and PI indicating that these two proteins function in a positive autoregulatory loop. (oboolo.com)
  • Photosynthesis occurs mainly in chloroplasts, whose development is regulated by proteins encoded by nuclear genes. (chinaagrisci.com)
  • Among them, pentapeptide repeat (PPR) proteins participate in organelle RNA editing. (chinaagrisci.com)
  • The osppr9 mutants were obtained by CRISPR/Cas9, which showed yellowing leaves and a lethal phenotype, with suppressed expression of genes associated with chloroplast development and accumulation of photosynthetic-related proteins. (chinaagrisci.com)
  • MiRNA expression is frequently altered during cancer development, associated with dysregulated expression of a plethora of different miRNAs, their biogenesis or processing proteins, such as DROSHA and DICER1 ( 1 ). (amegroups.org)
  • Finally, we discuss clinical significance of the CRISPR-Cas9 gene editing system and inhibitors of SE-related proteins such as BET and CDK7 as potential cancer therapeutics. (ijbs.com)
  • MiRNAs are generated by stepwise cleavage via double-stranded ribonuclease III enzymes DROSHA and DICER1 and loaded onto Argonaute (AGO) proteins to pair with target sequences on mRNAs 20 . (nature.com)
  • Real-time PCR and western blotting were carried out to detect the expressions of genes and proteins related to epithelial-mesenchymal transition (EMT). (biomedcentral.com)
  • Given the limitations of serum creatinine as a biomarker of kidney function, different urinary and serum proteins, molecules, and, most recently, microRNAs have been rigorously investigated over the past decade as possible biomarkers for kidney disease. (medscape.com)
  • MicroRNAs (miRNAs) are an abundant class of small single-stranded non-coding RNA molecules ranging from 18 to 24 nucleotides. (pasteur.fr)
  • BioGenex miRNA probes are dual-end labeled on 3' and 5' with five FAM molecules that amplify the signal, giving intense stains. (biogenex.com)
  • MicroRNAs (miRNAs) are approximately 22 nucleotide single-stranded RNA molecules that originate from hairpin precursors and regulate gene expression at the posttranscriptional level by basepairing with target messenger RNA and blocking its translation or inducing its degradation. (knaw.nl)
  • MicroRNAs (miRNAs) represent a class of naturally occurring small non-coding RNA molecules. (uni-luebeck.de)
  • As might be expected from molecules with these roles, miRNAs are involved in cancer development, and deregulation of several miRNAs has been found in various cancer types. (uni-luebeck.de)
  • Circular RNAs (circRNAs) belong to a class of non‐coding RNAs that are widespread in the cytoplasm of eukaryotic cells and are structurally and functionally different from linear RNA molecules [ 1 ]. (biomedcentral.com)
  • They are covalently closed-loop RNA molecules that are formed by back-splicing of the 5′ and 3′ ends of the primary transcript. (biomedcentral.com)
  • MicroRNAs are an abundant class of small regulatory RNA molecules about 19-22 nucleotides (nt) in length. (mirtar2go.org)
  • Previous studies have demonstrated that microRNAs (miRs) serve important roles in the progression of human cancer types, including pancreatic cancer (PC), a highly lethal malignancy. (spandidos-publications.com)
  • However, to the best of our knowledge, the association between miR‑205 expression and the progression of PC has rarely been investigated. (spandidos-publications.com)
  • miR-205 expression in humans was validated by Landgraf et al ( 8 ), however, its role in tumor progression is contradictory ( 9 - 12 ). (spandidos-publications.com)
  • Previous studies mainly focused on its roles in metabolism, but recently it was reported to be correlated with cancer progression and metastasis ( 5 ). (spandidos-publications.com)
  • For CRC, polymorphisms in the genes involved in tumor progression, apoptosis, and angiogenesis have already been extensively studied for their association with cancer susceptibility and prognosis ( 1 - 7 ). (iiarjournals.org)
  • The aim of this study is to investigate the key regulatory miRNA-486-5p and underlying molecular mechanisms in chronic obstructive pulmonary disease (COPD) progression. (researchsquare.com)
  • This miRNA functions as a tumor suppressor and dysregulation or loss of the host gene from which this miRNA is processed is associated with cancer progression in numerous cell types. (cancerindex.org)
  • The aim of our study was to evaluate if there is differential microRNA (miRNA) expression in various tumor subtypes during this progression. (openaccesspub.org)
  • In cancer, dysregulation of miRNAs can facilitate disease progression and therapeutic resistance, affecting tumour-suppressors and oncogenes. (amegroups.org)
  • These miRNA alterations are potential diagnostic and prognostic biomarkers when assessing patient disease progression or guiding clinical management. (amegroups.org)
  • We highlight the role of SE-driven genes, enhancer RNAs (eRNAs), lncRNAs, and miRNAs in the digestive system tumor growth and progression. (ijbs.com)
  • The core components of RNA silencing machinery involve several evolutionarily conserved protein families, including DICER (DCR) or DICER-LIKE (DCL), ARGONAUTE (AGO), and, in some cases, RNA-DEPENDENT RNA POLYMERASE (RDR). (ttu.edu)
  • miRNAs are small, evolutionarily conserved, noncoding RNAs that negatively regulate gene expression and have fundamental roles in cancer growth and metastasis. (biogenex.com)
  • These results demonstrate the evolutionarily conserved mode of action for miRNAs, but details of the mechanism diverge between the plant and metazoan kingdoms. (freethoughtblogs.com)
  • Our data indicate that the evolutionarily conserved miRNA pathway can be exploited by cancer cells to escape from T cell-mediated elimination and immunotherapy. (nature.com)
  • This study aims to investigate the potential role of miRNAs in equine osteochondrosis (OC) physiopathology.Short-read NGS technology (SOLID™, Life Technologies) was used to establish a comprehensive repertoire of miRNA expressed in either equine cartilage or subchondral bone. (pasteur.fr)
  • Despite their demonstrated roles in age-associated pathologies, little is known about the role of miRNAs in human aging and longevity. (altmetric.com)
  • This is a methodological pilot study of a prospective ovarian cancer patient cohort investigating the potential of liquid biopsies and the role of miRNAs in ovarian cancer treatment. (biomedcentral.com)
  • This study is a pilot part of the ongoing registered prospective CHEMOVA study, which aims at analyzing the role of miRNAs in prediction of primary treatment response and in the development of chemoresistance in high-grade serous ovarian cancer (HGSOC). (biomedcentral.com)
  • Xie Z . (2011) Biogenesis and function of virus-derived small interfering RNAs in plants. (ttu.edu)
  • Xie Z . (2010) Piecing the Puzzle Together: Genetic Requirements for miRNA Biogenesis in Arabidopsis thaliana . (ttu.edu)
  • Qi X, Bao FS, and Xie Z. (2009) Small RNA Deep Sequencing Reveals Role for Arabidopsis thaliana RNA-Dependent RNA Polymerases in Viral siRNA Biogenesis. (ttu.edu)
  • 2005) microRNA-directed phasing during trans-acting siRNA biogenesis in plants. (ttu.edu)
  • We find that the core microRNA (miRNA) biogenesis and targeting machinery maintains the sensitivity of cancer cells to PD-1-independent T cell-mediated cytotoxicity. (nature.com)
  • Research in my laboratory is directed to understand how multiple small RNA pathways operate in plants using genetic, genomic, and biochemical approaches. (ttu.edu)
  • Plants, therefore, provide a unique system to study the genetic diversification and functional adaptation of small RNA pathways. (ttu.edu)
  • Xie Z , and Qi X. (2008) Diverse small RNA-directed silencing pathways in plants. (ttu.edu)
  • 2004) Genetic and functional diversification of small RNA pathways in plants. (ttu.edu)
  • In this review, we focus on the impact of ncRNA post-transcriptional regulatory mechanisms, especially those of microRNAs and lncRNAs, in RA signalling pathways during differentiation and disease. (mdpi.com)
  • What pathways are this gene/protein implicaed in? (cancerindex.org)
  • We aimed to identify molecular pathways and genomic regulatory regions associated with altered miRNA expression due to glycemic status, which could contribute to the development of type 2 diabetes (T2D). (lu.se)
  • Expression pattern analysis of miRNAs helps to identify potential genetic interactors that exhibit similar expression patterns and this, combined with further supporting experiments, helps to identify the genetic pathways in which the specific miRNAs are involved. (knaw.nl)
  • This article summarises the current literature describing the impact of miRNAs on prediction and modification of anticancer treatment including the possible intracellular pathways involved in these processes. (uni-luebeck.de)
  • Our objective is to identify the related miRNAs and their associations with genes frequently involved in CRC microsatellite instability (MSI) and chromosomal instability (CIN) signaling pathways. (edu.hk)
  • A regression model was adopted to identify the significantly associated miRNAs targeting a set of candidate genes frequently involved in colorectal cancer MSI and CIN pathways. (edu.hk)
  • The results support significant roles for miR-126 in regulating cardiac myocyte survival pathways and cell death during exposure to simulated ischemia and acidosis. (biomedres.us)
  • Given the importance of miRNAs in regulating gene expression in other metazoans, further expression analyses of small non-coding RNAs in transcriptional studies of corals should be informative about miRNA-affected processes and pathways. (pasteur.fr)
  • Investigation of hub genes for the development of potential therapeutic targets and candidate biomarkers is warranted. (nature.com)
  • Finally, we identified 76 therapeutic agents, 152 miRNAs targets, and 91 TFs regulatory networks. (nature.com)
  • Recently, miRNAs have been studied as potential diagnostic or therapeutic targets in cancer treatment. (uni-luebeck.de)
  • Among other targets of miR-7 are insulin-like growth factor 1 receptor (IGF1R) and PIK3CD, E(spl) gene family and Pak1 (cancer cells). (wikipedia.org)
  • Analysis and clustering of highly correlated, experimentally validated gene targets of these miRNAs revealed two islet-specific clusters, which were associated with key aspects of islet functions and included a high number of T2D-related genes. (lu.se)
  • Several computational methods and algorithms have been developed to predict miRNA targets. (geneticsmr.org)
  • Therefore, miRNAs are ideal targets for anticancer drug development. (cancer-research-network.com)
  • 48 h) significantly reverses the expression of miR-34a downstream gene targets of DU145-TXR and PC3-TXR cell lines. (cancer-research-network.com)
  • Examination of putative miRNA gene targets indicates potential roles in development, metabolism, immunity, and biomineralisation for several of the microRNAs. (pasteur.fr)
  • MicroRNAs (miRNAs, miRs) are endogenously expressed small non-coding RNAs that generally regulate gene expression by binding to the 3ʹ untranslated region (3ʹ-UTR) of their targets, resulting in silencing [ 13 ]. (biomedcentral.com)
  • NEW: Analysis of the user's personal CLIP-Seq to find microRNA targets is also provided upon request (more in documentation page). (mirtar2go.org)
  • Furthermore, microRNAs are also shown to be targets for asthma therapy in the future. (cdc.gov)
  • Xie Z , Khanna K, Ruan S. (2010) Expression of MicroRNAs and Its Regulation in Plants. (ttu.edu)
  • The gene expression programs that establish and maintain cellular and organism homeostasis require precise, potent, and multifaceted forms of regulation. (k-state.edu)
  • We have only known that microRNAs even existed since the early 1990's, and their importance in gene regulation and development wasn't appreciated until the 2000's. (freethoughtblogs.com)
  • microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. (cancerindex.org)
  • Regulation of AP1 expression. (oboolo.com)
  • All elements required for proper spatial and temporal regulation of AG expression are contained within this intron. (oboolo.com)
  • MicroRNAs (miRNAs) are short non-coding RNAs that have been termed "master regulators of the genome" given their significance in post-transcriptional gene regulation, and roles in a multitude of normal and disease processes. (amegroups.org)
  • We found 1100 differentially expressed circRNAs (675 downregulated and 425 upregulated) which were involved in various biological processes such as protein ubiquitination modification, cell cycle regulation, RNA transport, and autophagy. (biomedcentral.com)
  • Combinatorial regulation is a feature of miRNA regulation in animals. (microrna1.com)
  • MicroRNAs not only participate in determining DCs phenotype and then naive T lymphocyte differentiation, but also participate in the regulation of airway inflammation and airway remodeling in asthma. (cdc.gov)
  • Zhang et al cytokines, mediators and signals and closely related to demonstrated that microRNA let-7i was upregulated immune regulation. (cdc.gov)
  • DC demonstrated that miRNAs participate in airway maturation, while downregulation of let-7i significantly inflammatory regulation in asthma. (cdc.gov)
  • More so, our results show that the epigenetic regulation of TE transcription is dynamically regulated throughout life (Paper II), upon the beginning of neuroinflammation (Paper III), and in a disease-driving polymorphic TE insertion (Paper IV). (lu.se)
  • MicroRNAs (miRNAs) are ~22-nt non-coding RNAs that negatively regulate gene expression at the post-transciptional level via direct binding with the 3′ untranslated regions (UTR) of target mRNAs ( 16 , 17 ). (spandidos-publications.com)
  • In particular, most miRNAs bind to target sequences located within the 3'-untranslated region (3'UTR) of mRNAs, resulting in the cleavage of the target mRNAs or repression of their translation ( 13 ). (iiarjournals.org)
  • MicroRNAs (miRNAs) are small non-coding RNAs that bind complementary messenger RNAs (mRNAs) to repress gene expression and regulate essentially all cellular processes 19 . (nature.com)
  • Xie Z ., Cheng H. (2017) Interplay and transition between small RNA-directed posttranscriptional and transcriptional gene silencing in plants. (ttu.edu)
  • TRIM13 inhibited cell proliferation and induced cell apoptosis by regulating NF-κB pathway in non-small-cell lung carcinoma cells. (cancerindex.org)
  • They regulate gene expression at the post-transcriptional level and control thereby cellular mechanisms including developmental transitions, organ morphology, apoptosis and cell proliferation. (uni-luebeck.de)
  • Micro-RNAs have been demonstrated to have multiple roles in regulating embryonic development, proliferation, apoptosis, etc. (openaccesspub.org)
  • Moreover, in cancer cells, miRNAs might function as oncogenes or tumor suppressors and their roles are manifested in almost all aspects of cancer biology including proliferation, apoptosis, invasion/metastasis, and angiogenesis. (cancer-research-network.com)
  • Here we investigated roles for miR-126 in stress kinase activation, induction of inflammatory cytokines and apoptosis caused by exposure of cultured cardiac myocytes to hypoxia/acidosis or acidosis alone. (biomedres.us)
  • Obligatory roles for acidosis in promoting apoptosis confirmed our previous reports that hypoxia alone does not confer a lethal signal. (biomedres.us)
  • Acidosis with or without hypoxia increased apoptosis that was paralleled by elevated miR-126, increased phosphorylation of p38-MAPK and JNK, enhanced expression of IL-6, IL-8, and TNF, and downregulation of Bcl-2. (biomedres.us)
  • The biological function of the differentially expressed circRNAs was determined by protein interaction network, Gene Ontology (GO), and Kyoto Encyclopedia of Gene and Genome (KEGG) pathway enrichment. (biomedcentral.com)
  • Key apoptotic pathway intermediates and inflammation markers IL-6, IL-8, and tumor necrosis factor (TNF) were quantified by western blot analyses, while miR-126 expression was tracked by quantitative polymerase chain reaction (qPCR). (biomedres.us)
  • The multistep transformation of normal cells into malignant cells involves genetic and epigenetic alterations that promote the aberrant expression of critical oncogenes and tumor suppressor genes [ 1 , 2 ]. (ijbs.com)
  • In this study, we found a negative correlation of IRS-1 expression with tumor metastasis both in human tissue samples and in cell lines. (spandidos-publications.com)
  • The epithelial-mesenchymal transition (EMT) plays important roles in tumor metastasis. (spandidos-publications.com)
  • Thus, the pivotal roles of miR-455 in cell viability and metastasis in HCC still needed to be explored. (hindawi.com)
  • These miRNAs have been implicated in critical NPC processes such as epithelial-to-mesenchymal transition (EMT) and metastasis, while further promoting chemoresistance and radioresistance. (amegroups.org)
  • Finally, TGF-β expression plasmids were transfected into NPC cells to verify the role of TGF-β in the miR-296-5p-mediated inhibition of nasopharyngeal carcinoma cell metastasis. (biomedcentral.com)
  • This provides new insight into the role of miR-296-5p in regulating NPC metastasis and invasiveness. (biomedcentral.com)
  • In this study, we established that miR-296-5p has a significantly lower expression in NPC tissues and cells and was involved in regulating the EMT and metastasis of NPC cells in vitro. (biomedcentral.com)
  • This study revealed a novel mechanism by which miR-296-5p regulates the EMT and metastasis of NPC cells, indicating that it could be a new target for the treatment of NPC. (biomedcentral.com)
  • First, with a profiling study, I identified a unique pattern of viral miRNA expression by normal persistently infected primary B cells in vivo and a subset of miRNAs that might be associated with cell growth and are deregulated in tumors. (tufts.edu)
  • Second, using optimized mouse cancer models, I found that the EBV BART miRNAs provide a growth/survival advantage to EBV-associated tumors in vivo therefore promoting tumor growth. (tufts.edu)
  • However, to the best of our knowledge, the miRs that regulate RUNX2 expression in tumors are unknown. (spandidos-publications.com)
  • Hundreds of dysregulated miRNAs have been found in studies comparing the expression profiles of miRNAs in malignant ovarian tumors and normal ovaries. (biomedcentral.com)
  • miR-494 and miR-125a-5p were found to be differentially regulated in tumors with an anaplastic component and even the well-differentiated component (FTC) of these tumors were found to be aligned with the anaplastic profile. (openaccesspub.org)
  • Recently, Nikiforva et al showed that miR-187 was the most up-regulated miRNA in tumors harboring RET/PTC rearrangements and RAS mutations, however it's expression is much lower in tumors with BRAF mutation 10 . (openaccesspub.org)
  • The core transcription regulatory loop of the digestive system tumors is complex and a high density of transcription regulatory complexes in the SEs and the crosstalk between SEs and the noncoding RNAs. (ijbs.com)
  • Furthermore, we discuss the oncogenic roles and regulatory mechanisms of SEs in the digestive system tumors. (ijbs.com)
  • The other approach is to screen CRISPR-Cas9-based guide RNA (gRNA) libraries that target either the whole genome using cancer cell and immune cell co-culture systems 9 , 10 , or focused gene sets using immunocompetent murine tumors 11 , 12 . (nature.com)
  • Research advances have demonstrated the crucial regulatory roles of miRNAs in many diseases, including COPD [ 13 , 14 ]. (researchsquare.com)
  • Taking into account the important regulatory roles that miRNAs have in cancer development, by acting either as oncogenes or as tumor-suppressor genes, they represent potential biomarkers in ovarian cancer [ 5 ]. (biomedcentral.com)
  • Circulating extracellular RNAs (ex-RNAs) are an emerging class of biomarkers with target-organ epigenetic effects. (researchgate.net)
  • In addition, miRNAs involvement in osteoarticular diseases has been proved and some of them were identified as suitable biomarkers for pathological conditions. (pasteur.fr)
  • Owing to their stability in biological fluids and resistance to various storage conditions, miRNAs are considered as useful biomarkers for cancer diagnosis, prognosis, and prediction of treatment efficacy. (biogenex.com)
  • Recent studies have tested miRNAs as predictive biomarkers for determining BRCA mutated patient's prognosis. (biogenex.com)
  • This review integrates the literature on EBV-miRNAs, cellular miRNAs, and miRNA signatures to guide future research, while acknowledging challenges in the selection and clinical implementation of relevant miRNA biomarkers and signatures. (amegroups.org)
  • How Reliable Are Gene Expression-Based and Immunohistochemical Biomarkers Assessed on a Core-Needle Biopsy? (lu.se)
  • 2004) Evolution of microRNA genes by inverted duplication of target gene sequences in Arabidopsis thaliana . (ttu.edu)
  • MiRNAs are most often identified through computational prediction from genome sequences. (usda.gov)
  • 50 with conserved sequences matching previously identified miRNAs and 4 novel miRNAs. (usda.gov)
  • The subsequent huge changes in morphology were more often due to changes in when and where those genes were expressed than to changes in the coding sequences of the genes themselves. (freethoughtblogs.com)
  • In addition, several miRNAs were shown to influence sensitivity to chemo- or radiotherapy: miRNAs of the Let-7 family induced radiosensitivity in vitro/in vivo, inhibition of miR-21 and miR-200b increased sensitivity to gemcitabine in cholangiocarcinoma cell lines, and restoration of miR-34 in p53-deficient human gastric cancer cells induced chemosensitisation. (uni-luebeck.de)
  • MicroRNAs (miRNAs or miRs) are small non-coding RNAs that participate in diverse cellular processes and negatively regulate gene expression at the post-transcriptional level by binding with 3′-untranslated regions (3′-UTRs) ( 2 - 4 ). (spandidos-publications.com)
  • They negatively regulate gene expression at the post-transcriptional level and play key roles in many biological processes, including skeletal development and cartilage maturation. (pasteur.fr)
  • MicroRNAs (miRNAs) are a class of short noncoding RNAs that negatively regulate gene expression. (cancer-research-network.com)
  • I am molecular and cellular biologist with expertise in virus and microRNAs. (researchgate.net)
  • miR-34a was found to be up-regulated in small airway fibroblasts in COPD patients compared to non-smokers, and miR-34a could promote cellular senescence in small airway fibroblasts in COPD patients [ 15 ]. (researchsquare.com)
  • To further understand the cellular programs essential for HCL ontogeny, we profiled the gene expression of leukemic cells isolated from BRAF(V600E)KI and Trp53KO or pTENKO mice, and found that they had similar but different gene expression signatures that resemble that of M2 or M1 macrophages. (bvsalud.org)
  • MiRNAs are approximately 19- to 25-nucleotide estimated about 250,000 and mortality does not appear single-stranded, noncoding RNAs that exist in both to correlate well with prevalence. (cdc.gov)
  • The EBV genome encodes more than 40 mature miRNAs identified to date. (tufts.edu)
  • In addition, more than 2,500 mature miRNAs in the human genome and miRNAs can regulate approximately 25%-70% of human genes. (cancer-research-network.com)
  • Using BioGenex ISH miRNA probes, the expression pattern of distinct miRNAs were successfully differentiated in wild type and BRCA mutated breast carcinomas. (biogenex.com)
  • While long noncoding RNAs (lncRNAs) are important i. (researchgate.net)
  • While long noncoding RNAs (lncRNAs) are important in cardiac development and disease, their role in physiol. (researchgate.net)
  • Long noncoding-RNAs (lncRNAs) are critical regulators of cardiac development as well as pathological hypertrophy and heart failure (HF). (researchgate.net)
  • Here, we used RNAseq to identify a novel class of cardiac lncRNAs that are dynamically regulated by exercise. (researchgate.net)
  • Moreover, some recent studies have demonstrated a relationship between the aberrant expression of miRNAs and CRC susceptibility, prognosis, and responsiveness to treatment ( 10 - 12 ). (iiarjournals.org)
  • Genetic susceptibility to breast cancer is highly increased with mutations in breast cancer susceptibility genes (BRCA1 and BRCA2). (biogenex.com)
  • The results of these analyses revealed that the differentially expressed exosomal miRNAs participate in multiple biological processes, such as gene expression, synthesis of biomolecules, cell development, differentiation, and signal transduction, among others. (hindawi.com)
  • Moreover, we found that these differentially expressed exosomal miRNAs connect osteogenic differentiation to processes such as axon guidance, MAPK signaling, and Wnt signaling. (hindawi.com)
  • Most of these paracrine secretions include soluble factors and exosomes, which regulate the repair and regeneration processes at sites of damage by affecting cell proliferation, migration, and differentiation [ 22 , 23 ]. (hindawi.com)
  • MiRNAs are known to control diverse processes including animal development and growth, cell differentiation, signal transduction, cancer disease, virus-induced immune defense, programmed cell death, insulin secretion and metabolism. (usda.gov)
  • Our results suggest that their is great potential for miRNAs to affect biochemical processes underlying important aquaculture production traits. (usda.gov)
  • miRNAs play essential roles in diverse biological and pathological processes, including development of hepatic fibrosis. (shengsci.com)
  • Previous studies have revealed the miR164 family and the miR164-targeted NAC transcription factor genes in rice ( Oryza sativa ) and Arabidopsis that play versatile roles in developmental processes and stress responses. (chinaagrisci.com)
  • They have been predicted to regulate the expression of more than 60% of mammalian genes and play fundamental roles in most biological processes including diseases. (mirtar2go.org)
  • Aberrant miRNA expression in smokers compared to non-smokers and COPD compared to normal was analyzed using microarray datasets and reverse-transcriptase quantitative polymerase chain reaction (qPCR). (researchsquare.com)
  • miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. (cancerindex.org)
  • SEs are defined as large clusters of active enhancers in large clusters that are enriched with high levels of transcription factors (TFs), master co-factors, mediator complexes, RNA polymerase II (RNA pol II), and epigenetic modifications [ 5 , 7 ]. (ijbs.com)
  • miRNAs are transcribed as ~70 nucleotide precursors (modelled here) and subsequently processed by the Dicer enzyme to give a ~22 nucleotide product. (wikipedia.org)
  • Mature miRNA-7 is derived from three microRNA precursors in the human genome, miR-7-1, miR-7-2 and miR-7-3. (wikipedia.org)
  • The rainbow trout genome sequence is not available yet, which does not allow miRNA prediction for this species which is of great economic interest for aquaculture and sport fisheries, and is a model research organism for studies related to carcinogenesis, toxicology, comparative immunology, disease ecology, physiology and nutrition. (usda.gov)
  • Conclusion: The rainbow trout miRNAs identified and characterized in this study provide a new tool for functional genome research in salmonids. (usda.gov)
  • It is one of the largest gene families accounting for approximately 1% of the genome and regulate approximately 1/3rd of all human genes 4 , 5 , 6 . (openaccesspub.org)
  • In this study, we described a method that can be used to integrate miRNA target prediction data from multiple sources and gene expression data to predict target genes of particular miRNAs. (geneticsmr.org)
  • A total of 5645 target genes of hsa-miR-375 were identified from five prediction programs, and among them, 2440 target genes were shared by at least 2 of these 5 programs. (geneticsmr.org)
  • Potential miRNA-target interactions were computationally predicted and single nucleotide polymorphisms (SNPs) were identified in the miRNAs and their target sites in the rainbow trout transcripts. (usda.gov)
  • Identification of sequence variations in the miRNA -target interactions may be particularly useful for rainbow trout breeding programs. (usda.gov)
  • miRTar2GO ranks the interactions predicted for a miRNA based on its distance to the verified interactions of that miRNA. (mirtar2go.org)
  • A number of studies have demonstrated that altered expression of miRs serves critical roles in human cancers by directly regulating cell behaviors ( 5 - 7 ). (spandidos-publications.com)
  • Some miRNAs modulate expression of known oncogenes or tumour suppressor genes whereas others function as so called onco-miRs or tumour-suppressor-miRs. (uni-luebeck.de)
  • Our results have laid down a solid foundation in exploration of novel CRC mechanisms, and identification of miRNA roles as oncomirs or tumor suppressor mirs in CRC. (edu.hk)
  • And indeed, different miRNAs have been found to predict sensitivity to anticancer treatment: miR-30c, miR-130a and miR-335 are downregulated in various chemoresistant cell lines, hsa-Let-7g and hsa-miR-181b are strongly associated with response to 5-fluorouracil-based antimetabolite S-1. (uni-luebeck.de)
  • The differentially expressed genes (DEGs) in AD were screened in GSE48350 dataset. (nature.com)
  • The differentially expressed oxidative stress genes (DEOSGs) were analyzed by intersection of DEGs and oxidative stress-related genes. (nature.com)
  • BioGenex unique SSNA miRNA ISH probes enable detection of single nucleotide mismatch thus allowing high sensitivity and specificity with a clean intense staining. (biogenex.com)
  • The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. (cancerindex.org)
  • BioGenex miRNA ISH Panel Probes are currently one of the best products for complex diagnostic assays. (biogenex.com)
  • The expressions of miR-296-5p in NPC tissues and cells were determined using GSE32920 database analysis and real-time PCR and miRNA microarray assays. (biomedcentral.com)
  • Based on small RNA sequencing, we present evidence for 31 bona fide microRNAs, 5 of which (miR-100, miR-2022, miR-2023, miR-2030, and miR-2036) are conserved in other metazoans. (pasteur.fr)
  • miR-7 is directly regulated by the transcription factor HoxD10. (wikipedia.org)
  • Using bioinformatics, a luciferase reporter assay and western blot analyses, the current study identified that runt‑related transcription factor 2 (RUNX2) was a target of miR‑205 in PC and overexpression of miR‑205 suppressed the expression of RUNX2. (spandidos-publications.com)
  • In addition, we examined the expression signature of transcription factors/regulators required for germinal center reaction and memory B cell versus plasma cell differentiation in these leukemic cells and found that most transcription factors/regulators essential for these programs were severely inhibited, illustrating why hairy cells are arrested at a transitional stage between activated B cells and memory B cells. (bvsalud.org)
  • LFY can directly regulate the transcription of at least one representative of each of the class A, B and C genes but uses distinct mechanisms to activate expression of each of these genes in a region-specific manner. (oboolo.com)
  • Finally, we constructed the drug, transcription factors (TFs), and microRNA network of the diagnostic genes. (nature.com)
  • We review related studies on mouse Adarl mutant phenotypes, their rescues by preventing signaling from the antiviral RIG-I-like Sensors (RLRs), as well as Marl mechanisms in innate immune suppression and other roles of Adarl, including editing-independent effects. (muni.cz)
  • 2004) Role of Arabidopsis ARGONAUTE4 in RNA-directed DNA methylation triggered by inverted repeats. (ttu.edu)
  • These studies have also addressed the various diagnostic and prognostic implications of micro-RNAs in thyroid carcinomas. (openaccesspub.org)
  • Enrichment analysis was performed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes. (nature.com)
  • In addition, 13 miRNAs were computationally predicted from the rainbow trout transcriptome. (usda.gov)
  • Cross-linking immunoprecipitation (CLIP) using AGO specific antibodies has been used to experimentally identify the AGO bound transcriptome that includes transcripts which are possibly targeted by miRNAs. (mirtar2go.org)
  • No physical interaction or cooperation of binding was observed in gel shift experiments Transcriptional repression of AG The lack of AG expression in vegetative tissues and the outer two floral whorls is a consequence of both the absence of AG activators and the activity of AG repressors. (oboolo.com)
  • Translational repression of AP2 The only floral organ-identity gene that does not encode a MADS-domain protein is the class A gene AP2. (oboolo.com)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • ADAR1 mutations lead to aberrant expression of interferon in Aicardi Goutieres syndrome (AGS), a human congenital encephalopathy. (muni.cz)
  • If this is the case, gene-correction presents a solution for patients with known monogenetic mutations. (lu.se)
  • Dysregulation of miRNA has been widely reported in many cancer types, including breast cancer. (biogenex.com)
  • In the post-genomic era, the cancer research is focused on the dysregulation of transcriptional dysregulation mediated by epigenetic modifications in the enhancer, SE, and gene promoter regions of key tumor suppressor and tumor-promoting genes [ 5 , 6 ]. (ijbs.com)
  • The plant hormone gibberellin appears to be an important regulator of homeotic gene expression in later stages of flower development (Yu et al. (oboolo.com)
  • however, with the rapid development of high-throughput sequencing technologies and bioinformatics, there is growing evidence that circRNAs are involved in regulating a variety of important physiological functions [ 8 ]. (biomedcentral.com)
  • Important roles of inflammatory mediators in cardiac cell death by ischemia with or without reperfusion are well established [12-14]. (biomedres.us)
  • This study confirms that alterations in the expression of exosomal miRNAs can promote osteogenic differentiation of ADSCs, which also provides the foundation for further research on the regulatory functions of exosomal miRNAs in the context of ADSC osteogenesis. (hindawi.com)
  • Insulin receptor substrate 1 (IRS-1) is an adaptor protein that integrates multiple transmembrane signals from growth factors and hormones, to regulate cell growth, survival, differentiation and metabolism ( 4 ). (spandidos-publications.com)
  • BioGenex is pleased to launch two Super Sensitive TM Nucleic Acid miRNA in situ hybridization (SSNA miRNA ISH) probes and automated systems for differentiation of wild type and BRCA mutated breast cancer. (biogenex.com)
  • A dual luciferase reporter assay was used to identify whether TGF-β is the target gene of miR-296-5p. (biomedcentral.com)