• MicroRNAs (miRNAs or miRs) play an active role in the regulation of gene expression by modifying mRNA stability and translation, usually through specific albeit partial complementary binding to the 3′ untranslated region. (nature.com)
  • The aberrant expression of circulating microRNAs (miRNAs/miRs) is reported in many tumors and can provide insights into the pathogenesis of tumor development and progression. (frontiersin.org)
  • Multiple components of biological fluids are being investigated as potential disease-related markers, including microRNAs (miRs, miRNAs) ( 8 ). (frontiersin.org)
  • MicroRNAs (miRNAs/miRs) are endogenous, single-stranded, small (approximately 22 nucleotides in length) noncoding RNA molecules that play an important and ubiquitous role in regulating genes expression. (spandidos-publications.com)
  • Previous studies have demonstrated that microRNAs (miRs) serve important roles in the progression of human cancer types, including pancreatic cancer (PC), a highly lethal malignancy. (spandidos-publications.com)
  • MicroRNAs (miRNAs or miRs) are small non-coding RNAs that participate in diverse cellular processes and negatively regulate gene expression at the post-transcriptional level by binding with 3′-untranslated regions (3′-UTRs) ( 2 - 4 ). (spandidos-publications.com)
  • A number of studies have demonstrated that altered expression of miRs serves critical roles in human cancers by directly regulating cell behaviors ( 5 - 7 ). (spandidos-publications.com)
  • However, to the best of our knowledge, the miRs that regulate RUNX2 expression in tumors are unknown. (spandidos-publications.com)
  • The Ct values of the target miRs were normalized to the Ct values of U6 RNA before quantification using the 2 −ΔΔ Ct method. (biomedcentral.com)
  • miRNAs are thought to have regulatory roles through complementarity to mRNA. (wikipedia.org)
  • It plays an important role in controlling mRNA expression. (wikipedia.org)
  • Bioinformatic predictions suggest that the human EGFR mRNA 3'-untranslated region contains three microRNA-7 (miR-7) target sites, which are not conserved across mammals. (wikipedia.org)
  • Furthermore, the miRNAs correlated negatively with the immunohistochemical expression of NRF1 and NRF2 but positively with their respective mRNA. (hindawi.com)
  • MicroRNAs (miRNAs) are small noncoding RNAs that posttranscriptionally regulate gene expression by imperfect matching of mRNA [ 6 ]. (hindawi.com)
  • The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. (cancerindex.org)
  • MiRNAs typically bind to the 3′ untranslated region (UTR) of their mRNA targets and downregulate gene expression via mRNA degradation or translational inhibition ( 8 - 10 ). (spandidos-publications.com)
  • For the mRNA analysis, RNA (1 mg) was reverse-transcribed by using the PrimeScript RT reagent Kit with gDNA Eraser (Tiangen), the mRNA level of the AK4 gene was quantified using duplex-qRT-PCR analysis where the Taqman probes with different fluorescence for β-actin were used in the FTC-3000P. (biomedcentral.com)
  • miRNAs predominantly interact with mRNA targets through imperfect binding to motifs in target mRNA 3′-untranslated regions (3′UTRs) ( Bartel, 2009 ). (elifesciences.org)
  • The nature of imperfect binding specificity means that a single miRNA can regulate a large number of mRNA targets involved in complex cellular processes, thereby tightly controlling genetic networks during development and in response to stress ( Pocock, 2011 ). (elifesciences.org)
  • As binding of miRNAs to a target mRNA may result in an airway inflammatory disease closely correlates with either mRNA degradation or inhibition of protein immune regulation, more and more evidences suggest translation.1 More than 2000 miRNAs have been discovered in humans. (cdc.gov)
  • Pax6 translation in the lateral wall of the subventricular zone of developed mice is post-transcriptionally regulated by miRNA-7a mediated gene silencing, which is necessary to control the rate of dopaminergic neuron production in the olfactory bulb. (wikipedia.org)
  • miRNAs are short-chain single-stranded RNA that suppress protein synthesis post-transcriptionally. (nii.ac.jp)
  • 4 , 5 , 6 The idea, however, that miRNAs may play an important role in some human disease/disorders is gaining momentum, and as these molecules have the ability to silence many genes simultaneously, dysregulation of even a single miRNA can have a significant polygenic effect. (nature.com)
  • They can modulate various biochemical and physiological processes, including plant response and resistance to fungal pathogens, by regulating the expression of numerous genes. (researchsquare.com)
  • Among of these genes, miR-124 caught our attention in view of the finding that large number of microRNAs(MiRNAs) have been shown important roles in the development of different cancers [ 11 ]. (biomedcentral.com)
  • Numbers shown below represent number of publications held in OncomiRDB database for Oncogenic and Tumor-Suppressive MicroRNAs. (cancerindex.org)
  • Understanding the mechanism underlying the role of tumor stroma, especially cancer-associated fibroblasts (CAFs), during chemoresistance would significantly contribute to the clinical application of chemotherapy agents. (dovepress.com)
  • Cancer-associated fibroblasts (CAFs) have been demonstrated to be essential stromal components in the tumor microenvironment, regulating cancer cell growth, migration, invasion, and chemo-response through various mechanisms. (dovepress.com)
  • miR-205 expression in humans was validated by Landgraf et al ( 8 ), however, its role in tumor progression is contradictory ( 9 - 12 ). (spandidos-publications.com)
  • The expression profiling of miRNAs has already been used in cancer clinics as diagnostic and prognostic biomarkers to assess tumor development [ 4 ]. (biomedcentral.com)
  • MicroRNAs (miRNAs) are a category of small nonencoding RNAs of 22 nucleotides in length. (hindawi.com)
  • microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. (cancerindex.org)
  • They regulate gene expression through different mechanisms [ 10 ] and play a diverse role in many cellular processes [ 11 - 14 ]. (hindawi.com)
  • These single-stranded non-coding RNAs drive the post-transcriptional repression of gene expression ( 9 ) and can be released to the extracellular environment either as circulating cell-free molecules or encapsulated within extracellular vesicles (EVs). (frontiersin.org)
  • Micro RNAs are 21- to 24-nucleotide-long, non-coding RNA molecules that regulate gene expression at the post-transcriptional level. (researchsquare.com)
  • A microRNA is a small non-coding RNA molecule, which functions in the post-transcriptional regulation of gene expression by partially complementing with the 3′-untranslated regions (3'UTR) of specific mRNAs [ 14 ]. (biomedcentral.com)
  • This study indicates that CAFs-derived exosomal miRNA-130a may be a potential therapeutic target for cisplatin resistance in NSCLC. (dovepress.com)
  • Due to the central role of autophagy in the removal of aggregation-prone proteins, a better understanding of mechanisms controlling autophagy is essential for the identification of novel therapeutic opportunities for multiple disease states. (elifesciences.org)
  • Understanding the roles of Asthma is a worldwide problem, the prevalence of miRNAs in asthma pathogenesis may also aid to asthma ranged from 1% to 18% of the population in explore new therapeutic targets. (cdc.gov)
  • Mature miRNA-7 is derived from three microRNA precursors in the human genome, miR-7-1, miR-7-2 and miR-7-3. (wikipedia.org)
  • The miR-7 gene is found in most sequenced Urbilateria species, and the sequence of its mature miRNA product is perfectly conserved from annelids to humans, indicating a strong functional conservation. (wikipedia.org)
  • The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. (cancerindex.org)
  • Annotated database entry including the location and sequence of the mature miRNA sequence. (cancerindex.org)
  • We focused on several miRNAs expressed in endothelial cells. (nii.ac.jp)
  • All-trans retinoic acid (RA) is the most active metabolite of vitamin A. Several studies have described a pivotal role for RA signalling in different biological processes such as cell growth and differentiation, embryonic development and organogenesis. (mdpi.com)
  • A total of 63 differentially expressed miRNAs have been previously linked to metastatic melanoma, many of which are known to be associated with multiple different cancers [ 10 ]. (hindawi.com)
  • In response to infection with V. nonalfalfae , we found five known and two novel miRNAs that are differentially expressed in the susceptible cultivar and six known miRNAs in the resistant cultivar. (researchsquare.com)
  • Differentially expressed miRNAs target 49 transcripts and their gene ontology enrichment analysis showed that the susceptible cultivar responds by modulating the processes of protein localization and pigment synthesis, whereas the resistant cultivar responds by modulating transcription factors and hormone signalling. (researchsquare.com)
  • An increasing number of evidence suggests that miRNAs may act as potential biomarkers for pancreatic injury [ 19 - 23 ]. (hindawi.com)
  • Circulating extracellular RNAs (ex-RNAs) are an emerging class of biomarkers with target-organ epigenetic effects. (researchgate.net)
  • Multiple roles and targets of miR-7 as well as its expression pattern were linked to regulatory mechanisms and pathogenesis in glioblastoma, breast cancer and other types of cancers, as well as in schizophrenia and visual abnormalities. (wikipedia.org)
  • The aim of this study was to determine the expression of microRNA-127 (miR-127) in both rat models and patients of acute pancreatitis (AP) with lung injury (LI). (hindawi.com)
  • We aimed to evaluate the selected miRNAs expression based on bioinformatic analysis and their changes before and after an ultramar. (researchgate.net)
  • Altered expression of circular RNAs (circRNAs) has previously been investigated in breast cancer. (researchgate.net)
  • While miR-137 overexpression was not associated with an observable specific phenotype, downregulation by antisense morpholino and/or transgenic expression of miR-sponge RNA induced significant impairment of both embryonic and larval touch-sensitivity without compromising overall anatomical development. (nature.com)
  • We observed miR-137 expression and activity in sensory neurons including Rohon-Beard neurons and dorsal root ganglia, two neuronal cell types that confer touch-sensitivity in normal zebrafish, suggesting a role of these cell types in the observed phenotype. (nature.com)
  • We tested selected miRNAs on a training cohort and created a classifier by integrating miRNA expression and patients' clinical data. (frontiersin.org)
  • The present study explored the expression and potential role of TRIM13 in non-small-cell lung carcinoma (NSCLC). (cancerindex.org)
  • In our study, we identified miRNAs in hops and their expression patterns in the roots of susceptible and resistant hop cultivars in the early stages of infection with the fungus V. nonalfalfae . (researchsquare.com)
  • For detecting and quantifying the expression of specific miRNAs, RNA was reverse transcribed using a Bulge-Loop™ miRNA qRT-PCR Primer Set and quantified by SYBR Green-based real-time PCR analysis in the FTC-3000P PCR instrument. (biomedcentral.com)
  • On the other hand, we clarified the upstream mechanism regulating miR-124-3p expression in ESCC, which involves in the hypermethylation-silencing regulation mediated by DNA methyltransferase 1(DNMT1), which is of high expression in ESCC tissues and cell lines in the present study. (biomedcentral.com)
  • Although more and animals and plants and regulate gene/protein expression more progressions have been made about the through direct complementarity between their 5' region pathogenesis of asthma in recent years, the increasing and the 3' untranslated region of target mRNAs. (cdc.gov)
  • TRIM13 inhibited cell proliferation and induced cell apoptosis by regulating NF-κB pathway in non-small-cell lung carcinoma cells. (cancerindex.org)
  • Here, we report that the microRNA miR-1 regulates the autophagy pathway through conserved targeting of the orthologous T re-2/ B ub2/ C DC16 (TBC) Rab GTPase-activating proteins TBC-7 and TBC1D15 in Caenorhabditis elegans and mammalian cells, respectively. (elifesciences.org)
  • Furthermore, it has been shown that miRNAs have a substantial role in melanocyte and melanoma biology [ 8 ] and that they affect, for instance, melanoma cell proliferation, invasion, and migration [ 9 ]. (hindawi.com)
  • We have identified a single microRNA that controls glioma cell proliferation, migration, and responsiveness to glucose deprivation. (researchgate.net)
  • This family represents the microRNA (miRNA) precursor mir-7. (wikipedia.org)
  • 3 These molecules play a crucial role in development, particularly in the brain, where they are believed to be particularly important for stabilizing gene-regulatory networks during the transition between developmental states. (nature.com)
  • Although the roles of cytokines and chemokines in respiratory complications of AP were proposed by extensive studies in the last two decades, the definite mechanism is yet to be fully elucidated [ 4 ]. (hindawi.com)
  • In addition, Kayed et al ( 15 ) demonstrated that RUNX2 was overexpressed in PC and could be regulated by certain cytokines, including transforming growth factor β1 and bone morphogenetic protein 2. (spandidos-publications.com)
  • Zhang et al cytokines, mediators and signals and closely related to demonstrated that microRNA let-7i was upregulated immune regulation. (cdc.gov)
  • MicroRNAs participate in the regulation of asthma, the goal of this study is to summarize recent researches on the roles of microRNAs in the pathogenesis of asthma. (cdc.gov)
  • The prognostic significance of the major redox regulator nuclear factor erythroid-2-related factor (NRF2) is recognized in many cancers, but the role of NRF1 is not generally well understood in cancer. (hindawi.com)
  • Specifically, one type of miRNA is also involved in different cancers. (biomedcentral.com)
  • In this review, we focus on the impact of ncRNA post-transcriptional regulatory mechanisms, especially those of microRNAs and lncRNAs, in RA signalling pathways during differentiation and disease. (mdpi.com)
  • In addition, due to their rapid and reversible regulatory capacity, miRNAs are prime candidate facilitators of responses to proteotoxic stress. (elifesciences.org)
  • Extracellular small non-coding RNAs (exRNAs) have been associated with cardiac inflammation and fibrosis and we hypothesized that they are associated with post-MI LVRm phenotypes. (researchgate.net)
  • Small non-coding RNAs in particular have emerged as potential candidates as they can exert a broad influence on functional networks and have been found to be dysregulated in SCZ postmortem brains. (nature.com)
  • MiRNAs (miRNAs) are small non-coding RNAs which are recognized as vital and evolutionarily ancient components of gene regulation [ 1 ]. (biomedcentral.com)
  • 1 , 2 Most miRNAs are predicted to regulate several hundred mRNAs, and many mRNAs are regulated by multiple miRNAs. (nature.com)
  • Our study thus clarifies a new mechanism that DNMT1/miR-124/BCAT1 axis regulates the development and progression of ESCC. (biomedcentral.com)
  • These findings prompted us to investigate the role of DNMT/miR-124/BCAT1 axis in regulating development and progression of ESCC. (biomedcentral.com)
  • This study proposes a miRNA-based biomarker classifier for SCLC that considers clinical demographics with specific cut offs to inform SCLC diagnosis. (frontiersin.org)
  • Considering the potential role of miR-127 in the vital biological process of inflammation, we hypothesized that miR-127 may serve as a marker of AP with LI. (hindawi.com)
  • MicroRNAs not only participate in determining DCs phenotype and then naive T lymphocyte differentiation, but also participate in the regulation of airway inflammation and airway remodeling in asthma. (cdc.gov)
  • In particular, miR-23B, miR-93, miR-144, and miR-212 were found to play a role as NRF2 inhibitors, while miR-340 appears to have a role as an NRF1 and MAPK inhibitor with miR-383 and miR-510 having a less clear role in the regulation of NRF1 and NRF2 levels [ 7 ]. (hindawi.com)
  • An improvement in our understanding of the role that miRNAs play in AP may represent an attractive way to develop new diagnostic and prognostic tools for use in future clinical applications. (hindawi.com)
  • Endothelial cells play a central role in controlling vascular function and maintaining vascular integrity. (nii.ac.jp)
  • Exosomes play an essential role in intercellular communication. (dovepress.com)
  • TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. (cancerindex.org)
  • MicroRNAs (miRNAs) play vital roles in regulating various biological processes. (biomedcentral.com)
  • The results of the present study indicate that microRNAs play important roles in regulating asthma immune responses. (cdc.gov)
  • While long noncoding RNAs (lncRNAs) are important i. (researchgate.net)
  • While long noncoding RNAs (lncRNAs) are important in cardiac development and disease, their role in physiol. (researchgate.net)
  • Long noncoding-RNAs (lncRNAs) are critical regulators of cardiac development as well as pathological hypertrophy and heart failure (HF). (researchgate.net)
  • miRNAs are transcribed as ~70 nucleotide precursors (modelled here) and subsequently processed by the Dicer enzyme to give a ~22 nucleotide product. (wikipedia.org)
  • The involvement of Dicer in miRNA processing suggests a relationship with the phenomenon of RNA interference. (wikipedia.org)
  • miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. (cancerindex.org)
  • The discovery of misregulated miRNAs not only broadened our biological understanding of these diseases but also provided a new class of markers. (hindawi.com)
  • Up- or down-regulation of miR-199a-3p and/or the AK4 gene was done to detect their roles in OS multi-drug resistance using drug resistance profiling assays. (biomedcentral.com)
  • DC demonstrated that miRNAs participate in airway maturation, while downregulation of let-7i significantly inflammatory regulation in asthma. (cdc.gov)
  • Indeed, miRNAs have been shown to be actively involved in the coordination of almost all major cellular processes, such as differentiation ( 11 ), proliferation ( 12 ), metabolism, and apoptosis ( 13 ). (spandidos-publications.com)
  • Taken together, these results suggest that CAFs-derived exosomes confer cisplatin resistance of NSCLC cells through transferring miRNA-130a and that PUM2 is a critical factor for packaging miRNA-130a into exosomes. (dovepress.com)
  • The present study was designed to investigate the role of the miR‑133b‑5p in HPC‑induced cardioprotection and the underlying mechanisms involving caspase‑8 and caspase‑3 apoptotic signaling. (spandidos-publications.com)
  • To achieve the best effectiveness of the defence response, all defence mechanisms must be well regulated. (researchsquare.com)
  • The neurodevelopmentally regulated microRNA miR-137 was strongly implicated as risk locus for schizophrenia in the most recent genome wide association study coordinated by the Psychiatric Genome Consortium (PGC). (nature.com)
  • In a previous study using a microRNA (miRNA/miR) microarray assay, we demonstrated that miR-133b-5p was upregulated in response to hypoxic preconditioning (HPC). (spandidos-publications.com)
  • The results of our study suggest that the susceptible and the resistant cultivar respond differently at the miRNA level to infection with V. nonalfalfae and that miRNAs may contribute to the successful defence of the resistant cultivar. (researchsquare.com)
  • A review of the English medical literatures was conducted by searching PubMed for studies concerning asthma and microRNAs. (cdc.gov)
  • that asthma is intensively regulated by a variety of microRNAs (miRNAs). (cdc.gov)
  • Our aim was to investigate these redox transcription factors in conjunction with redox-related microRNAs in naevi and melanoma. (hindawi.com)
  • miR-127 is originally found to be highly expressed in human and murine embryos and has a critical role in lung development and placental formation [ 34 , 35 ]. (hindawi.com)
  • Loss of cardiomyocytes is a major cause of heart failure, and while the adult heart has a limited capacity for cardiomyogenesis, little is known about what regulates this ability or whether it can be effectively harnessed. (researchgate.net)