Loading...
  • targets
  • We performed more detailed experiments on two miRNAs, miR-974 and miR-31a, by mapping their roles to subpopulations of brain neurons and testing the functional involvement in memory of potential mRNA targets through bioinformatics and a RNA interference knockdown approach. (ox.ac.uk)
  • This screen offers an important first step toward the comprehensive identification of all miRNAs and their potential targets that serve in gene regulatory networks important for normal learning and memory. (ox.ac.uk)
  • roles
  • Here, we systematically surveyed 134 different miRNAs for roles in olfactory learning and memory formation using "sponge" technology to titrate their activity broadly in the Drosophila melanogaster central nervous system. (ox.ac.uk)
  • cellular
  • Prior studies have shown that they regulate numerous physiological processes critical for normal development, cellular growth control, and organismal behavior. (ox.ac.uk)
  • physiological
  • Whereas miRNA expression has been examined in lung tumors ( 18 - 24 ), neither the expression of miRNA in bronchial epithelium nor the effect of smoking on bronchial airway miRNA expression has been characterized, and it is unclear what role miRNAs play in regulating the physiological response to environmental exposures such as tobacco smoke. (pnas.org)
  • cancers
  • In solid tumors, such as tummy, pancreatic and prostate cancers, amendment of the known amounts of a little amount Crenolanib of miRNAs, including miR-214, provides been discovered as a personal for these tumors (10,11). (healthandwellnesssource.org)
  • Irregularities in miRNA-regulated gene expression have been found to be associated with cancers, cardiovascular disorders and a variety of other diseases. (ambriex.com.br)
  • Altered miRNA levels have been associated with diabetes, insulin resistance, inflammation and various cancers. (mdpi.com)
  • The altered miRNA expression profile has been proposed to correlate with the stage and survival in patients with various cancers, including colon, lung, breast, and pancreatic cancers [ 5 ]. (hindawi.com)
  • Aberrant miRNA profiles have been noted in various cancers ( 5, 6 ), including RCC ( 7-10 ). (aacrjournals.org)
  • Circulating MicroRNAs
  • In Circulating MicroRNAs: Methods and Protocols, expert researchers in the field detail recent advances in the isolation, purification and analysis of circulating miRNAs from a variety of sources for research. (springer.com)
  • Authoritative and practical, Circulating MicroRNAs: Methods and Protocols seeks to aid scientists in dealing with the recent advances of RNAi technology from the bench to the bedside. (springer.com)
  • progression
  • Elegant studies have demonstrated that miRNAs are involved in the initiation and progression of several malignancies. (springer.com)
  • Several studies have been conducted to identify the RCC-specific miRNA signature ( 7-10 ) though no consensus has been reached on which miRNAs are relevant for development and progression of this malignancy. (aacrjournals.org)
  • Serum
  • Emerging evidence underlines an involvement of miRNAs in the pathogenesis of Multiple Sclerosis (MS). A number of miRNAs have been found to be dysregulated in blood cells from MS patients, in brain lesions, as well as in biological fluids such as serum and plasma. (mdpi.com)
  • Despite miRNA altered expression likely showing a high tissue specificity, some profile similarities could be observed for certain miRNAs such as miR-326-such as upregulation in both active lesions and blood-though not for others such as miR-323, which demonstrated upregulation in whole blood, active brain lesions, and T-reg cells, but not in the serum of MS patients. (mdpi.com)
  • specificity
  • Moreover, researchers have exploited the special characteristics of miRNAs, such as the tissue and disease specificity or miRNA presence in blood, to explore their use as non-invasive tumour markers. (mdpi.com)
  • Dicer
  • The pre-miRNA is a double-stranded hairpin structure of RNA with 60-70 bp in length [ 3 ], which can be further processed into matured miRNA by RNaseIII dicer, ultimately form RNA-induced silencing complex (RISC) after exported to cytoplasm by exportin-5 [ 4 ]. (hindawi.com)
  • target mRNA
  • RISC can functionally inhibit gene expression by binding to the 3′ untranslated region (3′ UTR) in target mRNA, which is degraded if the miRNA:mRNA complex complementarity is perfect, or the translation is suppressed if the complementarity is not perfect. (hindawi.com)
  • formalin-fixed
  • This study investigated whether formalin-fixed paraffin-embedded (FFPE) specimens from postmortem cases of neurodegenerative disorders would be suitable for miRNA profiling. (biomedcentral.com)
  • proteins
  • Exosomes can fuse with the plasma membrane resulting in the transfer of RNA and proteins to the target cell. (jove.com)
  • exosomes
  • Here we will describe the procedure for quantitatively analyzing miRNAs and messenger RNAs (mRNA) from exosomes secreted in blood and cell culture media. (jove.com)
  • Total RNA will be purified from these exosomes to ensure that we can study both mRNA and miRNA from the same sample. (jove.com)
  • Our representative data show variations in miRNAs present in exosomes purified from mouse blood, human blood and human cell culture media. (jove.com)
  • expression patterns
  • MiRNAs mir-488, mir-503, and mir-647 were identified as potential reference miRNAs for future studies, based on the stability of their expression patterns across the 77 cases and the two case-groups. (aacrjournals.org)
  • qPCR
  • After validating RNA integrity by Bioanalyzer, we will perform a medium throughput quantitative real time PCR (qPCR) to identify the exosomal miRNA using Taqman Low Density Array (TLDA) cards and gene expression studies for transcripts of interest. (jove.com)
  • Each miRNA-specific primer used in the qPCR arrays has been experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted miRNA. (ambriex.com.br)
  • Using a universal real-time PCR condition, one can easily profile and analyze the miRNA expression in a high-throughput fashion.The All-in-One™ miRNA First-Strand cDNA Synthesis Kits and qPCR Mix Kits are the recommended and supported RT-PCR reagents for use with the miProfile miRNA qPCR arrays. (ambriex.com.br)