Mirna mimics. Medical search. Frequent questions

MicroRNAs (miRNAs or miRs) play an active role in the regulation of gene expression by modifying mRNA stability and translation, usually through specific albeit partial complementary binding to the 3′ untranslated region. (nature.com)
MicroRNAs (miRNAs) are a group of endogenous non-coding RNAs, which are single-stranded and ~21 nucleotides (~21 nt) in length. (nature.com)
In addition to protein-coding genes, the human genome contains a large number of genes which are transcribed to non-coding RNAs, including microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) ( 7 , 8 ). (spandidos-publications.com)
Recently, an increasing number of reports have implicated a new class of small regulatory RNA molecules, termed microRNAs (miRNAs), in HCC progression. (spandidos-publications.com)
MicroRNAs (miRNAs) are a kind of small noncoding RNAs that regulate the expression of protein through targeting the 3′UTR of messenger RNA (mRNA), resulting in mRNA degradation or suppression of translation [ 5 ]. (degruyter.com)
microRNAs (miRNAs) are important regulators of target messenger RNA translation by binding mainly to complementary sequences of the 3′ untranslated region of target messenger RNA transcripts thereby leading to RNA degradation and/or inhibition of protein synthesis [ 4 ]. (hindawi.com)
MicroRNAs (miRNAs) are small regulatory non-coding RNAs, resulting from the cleavage of long primary transcripts (pri-miRNAs) in the nucleus by the Microprocessor complex generating precursors (pre-miRNAs) that are then exported to the cytoplasm and processed into mature miRNAs. (mdpi.com)
Here we explore combinations of different classes of therapeutic anti-HIV-1 RNAs expressed from within the context of an intronic MCM7 (minichromosome maintenance complex component-7) platform that naturally harbors 3 microRNAs (miRNAs). (ca.gov)
The pathogenesis of myelodysplastic syndrome (MDS) may be related to the abnormal expression of microRNAs (miRNAs), which could influence the differentiation capacity of mesenchymal stem cells (MSCs) towards adipogenic and osteogenic lineages. (techscience.com)
that asthma is intensively regulated by a variety of microRNAs (miRNAs). (cdc.gov)

miRNA mimics and agomirs bind to the 3'UTR of genes to knock down native gene expression in cells. (abmgood.com)
4 , 5 , 6 The idea, however, that miRNAs may play an important role in some human disease/disorders is gaining momentum, and as these molecules have the ability to silence many genes simultaneously, dysregulation of even a single miRNA can have a significant polygenic effect. (nature.com)
Accumulating studies have suggested that miRNAs are frequently dysregulated in tumors compared with normal tissues, resulting in aberrant expression of target genes or proteins and cancer progression 13 . (nature.com)
Briefly, miRNAs are expected to account for 1-5% of the human genome and to interfere with at least 30% of the protein-coding genes ( 4 , 5 ). (frontiersin.org)
In most cases, circRNAs usually act as a competitive endogenous RNA (ceRNA) that can absorb miRNAs to regulate the expression of targeted genes [ 9 ]. (hindawi.com)
Identification of miRNA-regulated targeting genes is important for understanding their specific biological functions [ 11 ]. (degruyter.com)
Some miRNAs are hosted in pri-miRNAs annotated as long non-coding RNAs (lncRNAs) and defined as MIRHGs (for miRNA Host Genes). (mdpi.com)
As miR-24-3p is a regulatory molecule, Villanueva was keen to determine which genes the miRNA acted on were involved in cancer expression and identified two suspects: PRSS8 and HMOX1. (cornell.edu)
The miRIDIAN microRNA Mimic Housekeeping Positive Controls allows for direct monitoring of housekeeping genes in a microRNA mimic experiment. (horizondiscovery.com)
The miRIDIAN microRNA Mimic Housekeeping Positive Controls allow for the direct monitoring of housekeeping genes in a microRNA mimic experiment. (horizondiscovery.com)

miRNAs are highly conserved, small, non-coding RNAs that negatively regulate gene expression in vertebrates through multiple mechanisms, such as complimentary base pairing with the 3′-UTR of their target mRNAs, resulting in translational repression, mRNA cleavage and mRNA decay initiated by miRNA-guided rapid deadenylation ( 10 ). (spandidos-publications.com)
In this current study, we utilized an endogenous microRNA (miRNA) cluster as a platform to express multiple anti-HIV small RNAs. (ca.gov)
We demonstrate the versatility of the MCM7 platform in expressing and efficiently processing the siRNAs as miRNA mimics along with nucleolar small RNAs. (ca.gov)
Now, researchers at the Cornell University College of Veterinary Medicine have revealed in the December 2022 issue of BMC Genomics how different combinations of gene mutations can affect the activity of regulatory molecules, called micro RNAs (miRNAs), that in turn can shape colon tumor development. (cornell.edu)
They include coding mRNAs and non-coding (nc) RNAs among them antisense oligonucleotides (ASOs), small interfering RNAs (siRNAs), micro-RNAs (miRNAs), small activating RNAs (saRNAs), RNA aptamers and RNA guides. (frontiersin.org)
MiRNAs are approximately 19- to 25-nucleotide estimated about 250,000 and mortality does not appear single-stranded, noncoding RNAs that exist in both to correlate well with prevalence. (cdc.gov)

Can't find the miRNA or inhibitor you're looking for? (abmgood.com)
HBEpC were transfected with miRNAs inhibitor oligonucleotide or mimic oligonucleotide (Thermo Fisher Scientific, Carlsbad, CA) using the lipid-based Lipofectamine 2000. (cdc.gov)
An NPCIL1 genetic variant mimicking the effect of ezetimibe, an inhibitor of N PC 1L1, s associated with reduced low-density l ipoprotein(LDL) cholesterol and possibly with increased biliary cholesterol, a risk factor for gallstone disease. (p450inhibitors.com)

These miRNAs which targeted β -catenin mRNA were confirmed by dual-luciferase reporter system and RNA-pulldown. (hindawi.com)
In a previous work, increased expression of multiple miRNAs during TDI sensitization was observed and several putative mRNA targets identified for these miRNAs were directly related to regulatory T-cell (Treg) differentiation and function including Foxp3 and Runx3. (cdc.gov)
miRIDIAN microRNA Mimics designed to target the 3' UTR of either PPIB or GAPDH were transfected at 50 nM using DharmaFECT 1 into the indicated cell lines and assessed for their ability to decrease target mRNA levels. (horizondiscovery.com)
The miRIDIAN microRNA Mimic Endogenous Positive Control helps you monitor specific mimic effects on target protein levels in a validated endogenous assay, which is based upon the targeted activity of miR-122 on Aldolase A mRNA levels in cell lines. (horizondiscovery.com)
As binding of miRNAs to a target mRNA may result in an airway inflammatory disease closely correlates with either mRNA degradation or inhibition of protein immune regulation, more and more evidences suggest translation.1 More than 2000 miRNAs have been discovered in humans. (cdc.gov)

We have miRNA overexpression/mimics products and miRNA inhibitiors in synthetic, vector or virus formats to suit your experiment. (abmgood.com)
lncRNA‑NEF overexpression mediated the inhibition of miRNA‑21 expression in osteosarcoma cell lines, while miRNA‑21 overexpression did not significantly affect the expression of lncRNA‑NEF. (spandidos-publications.com)
lncRNA‑NEF overexpression inhibited, while miRNA‑21 overexpression promoted, migration and invasion of osteosarcoma cell lines in vitro. (spandidos-publications.com)
miRNA‑21 overexpression partially compensated the inhibitory effects of lncRNA‑NEF overexpression on osteosarcoma cell migration and invasion. (spandidos-publications.com)
Notably, miR-200's ability to do this was found to be dependent on tumor type, with its overexpression associated with poor clinical outcomes in certain cancers and positive outcomes in others - findings that have important translational implications for the development of miRNA-based therapeutics, according to the investigators. (genomeweb.com)
This led to significant overexpression of target miRNA in the kidney, resulting in inhibited progression of kidney disease in several mouse models. (jove.com)

SiRNAs of LINC00673, LINC00673, miRNA mimics and inhibitors were designed and synthesized by Ruibo Guangzhou. (jcancer.org)

miRNA Mimics are chemically synthesized, double-stranded miRNA-like RNA which are designed to copy the functionality of mature endogenous miRNA upon transfection. (abmgood.com)
To optimize experimental conditions, we offer both housekeeping and endogenous positive controls for gain-of-function experiments using miRIDIAN Mimics. (horizondiscovery.com)

The product mimics pri-microRNA (pri-miRNA) and is processed by Drosha. (wikipedia.org)
miRIDIAN microRNA Mimic Positive Controls are ideal for optimizing conditions for relevant, well-controlled microRNA modulation experiments. (horizondiscovery.com)

Also available are miRNA Agomirs, a type of miRNA mimic with chemical modifications to prevent degradation and increase transfection efficiency. (abmgood.com)
DEFB1 expression after transfection with hsa-miR-186-5p and hsa-miR-340-5p provided evidence that DEFB1 expression could be modulated by these two miRNAs and hsa-miR-186-5p had a higher binding efficiency with DEFB1 . (cdc.gov)
Transfection of MDS-MSCs with the miR-103-3p mimic improved osteogenic differentiation and decreased adipogenic differentiation in vitro, while inhibition of miR-103-3p showed the opposite results in NOR-MSCs. (techscience.com)
Transfection with candidate miRNAs mimics significantly decreased neuroblastoma cells proliferation rate. (oncotarget.com)

We offer a collection of synthetic miRNA Mimics and Agomirs for over 5000 mature miRNAs from human, mouse, and rat. (abmgood.com)

The aberrant expression of miRNAs is associated with the pathogenesis of sepsis. (degruyter.com)
Increasing evidence has suggested that the dysregulation of miRNAs is associated with the pathogenesis of sepsis. (degruyter.com)
The miR-103-3p mimics may boost MDS-MSCs osteogenic differentiation while weakening lipid differentiation, thereby providing possible target for the treatment of MDS pathogenesis. (techscience.com)
Involvement of several miRNAs in its pathogenesis has been ascertained. (oncotarget.com)
Understanding the roles of Asthma is a worldwide problem, the prevalence of miRNAs in asthma pathogenesis may also aid to asthma ranged from 1% to 18% of the population in explore new therapeutic targets. (cdc.gov)

1 , 2 Most miRNAs are predicted to regulate several hundred mRNAs, and many mRNAs are regulated by multiple miRNAs. (nature.com)

miRNAs have been implicated as transcriptional regulators in a wide range of biological processes determining cell fate, stress response, proliferation, or death [ 5 ]. (hindawi.com)
Thus, the team was interested to see how different combinations of oncogenic mutations affect miRNA regulators of colon tumor development. (cornell.edu)

Starting from a high-throughput expression profiling of 754 miRNAs and based on a proper selection, we focused on miR-29a-3p, miR-34b-3p, miR-181c-5p and miR-517a-3p as candidate miRNAs for our analysis. (oncotarget.com)

Indeed, it has been shown that miRNAs play an important role in gene expression, mainly when associated with the monitoring of several cell and metabolic pathways, being also an essential component of the gene silencing machinery in most eukaryotic organisms ( 4 , 8 ). (frontiersin.org)
A recent demonstration of the differential expression of miRNAs and their target mRNAs in cancer and the discovery that some miRNAs can function as oncogenes or tumor suppressors have sparked considerable interest in elucidating their role in tumorigenesis ( 5 , 6 ). (spandidos-publications.com)
These diversified gene mutation combinations result in aberrations in the molecular machinery that regulate the gene expression-like miRNAs. (cornell.edu)
Our work sheds light on how different mutational combinations affect miRNA expression. (cornell.edu)
Using small RNA-sequencing, the scientists identified how these different combinations of mutations affected the expression of tumor suppressor and oncogenic miRNAs. (cornell.edu)
The team began by analyzing miR-200 expression data for four adenocarcinomas and found that low expression of a member of the miRNA family was significantly associated with worse overall survival for ovarian and renal cancers. (genomeweb.com)
Cells derived from TDI sensitized mice treated with miR-210 mimic had less expression of miR-210 compared to the acetone control suggesting other factors, such as additional miRNAs, might be involved in the regulation of the functional capabilities of these cells. (cdc.gov)

As an oncogenic miRNA, miRNA-21 promotes tumor development in different types of cancer, including osteosarcoma ( 11 ). (spandidos-publications.com)

Overall, identification of novel biomarkers for TNBC is currently a high priority, and miRNAs as potential biomarkers, require better understanding of their roles and mechanisms in TNBC. (nature.com)
Identification of miRNAs panel can be thus considered an upcoming and potentially useful strategy in PCa diagnosis, given that radioresistance biomarkers, in both prognosis and therapy still remains a challenge. (frontiersin.org)
A novel high-resolution adaptation allows us to isolate microvesicles/exosomes from biofluids for the study of cancer biomarkers such as miRNA. (lu.se)

Additionally, 101 DE-lincRNAs were predicted as targets of miRNA by psRNATarget, while 5 target mimics were identified using target mimicry search in TAPIR. (biomedcentral.com)
Additional in vitro and in vivo experiments confirmed that IL-8 and CXCL1 are direct targets of the miR-200 family, and that the miRNA family can directly affect angiogenesis in endothelial cells by targeting production of IL-8 and CXCL1. (genomeweb.com)
Positive Control #2 targets GAPD so experimental conditions may be optimized with a validated mimic control. (horizondiscovery.com)

Unlike the amount of methylation of their encoding gene promoters, all these miRNAs were significantly overexpressed following treatment with 5'-AZA. (oncotarget.com)
DC demonstrated that miRNAs participate in airway maturation, while downregulation of let-7i significantly inflammatory regulation in asthma. (cdc.gov)

Moreover, exosomal miRNA-130a was transferred from CAFs to recipient NSCLC cells and knockdown of miRNA-130a reversed the effect of CAFs-derived exosomes during chemoresistance of NSCLC cells. (dovepress.com)
Furthermore, pumilio homolog 2 (PUM2), a RNA-binding protein, mediated the packaging of miRNA-130a into exosomes. (dovepress.com)
Taken together, these results suggest that CAFs-derived exosomes confer cisplatin resistance of NSCLC cells through transferring miRNA-130a and that PUM2 is a critical factor for packaging miRNA-130a into exosomes. (dovepress.com)

Small non-protein-coding RNA molecules, composed of around 22 nucleotides, are commonly named as miRNAs ( 1 - 3 ). (frontiersin.org)

The first miRNA was discovered in 1993 by Lee, Freinbaum and Ambros ( 6 , 7 ), and since then an increasing load of literature data have pointed that they can act as both tumor suppressors and oncogenes ( 1 - 3 ). (frontiersin.org)
Since their discovery in 1993, miRNAs have been described in all multicellular organisms and are associated with a vast breadth of biological functions, including cellular proliferation, cellular differentiation and immunity, as well as tissue remodeling and various human diseases, including cancer ( 4 ). (spandidos-publications.com)

Taken together, our findings indicate that the lincRNA of wheat responded to Bgt and Pst stress and played important roles in splicesome and inter-regulating with miRNA. (biomedcentral.com)
however, the roles of miRNAs in chemical sensitization are largely unknown. (cdc.gov)
Because the functional roles of miRNAs have not been previously elucidated in a model of chemical sensitization, these data contribute to the understanding of the potential immunologic mechanisms of chemical induced allergic disease. (cdc.gov)

Dysregulation of miRNAs is a common feature in human cancers, but this phenomenon has not been studied extensively in hepatocellular carcinoma (HCC). (spandidos-publications.com)

miRNAs and lncRNAs participate in the regulation of cell growth, proliferation, differentiation, migration and invasion by interacting with a number of signaling pathways ( 7 , 8 ). (spandidos-publications.com)

We offer synthetic miRNAs for human, mouse and rat, in the form of agomirs or mimics. (abmgood.com)

As main findings, 23 miRNAs were already identified as being involved in genetic regulation of PCa cell response to RT. (frontiersin.org)

However, recent studies have demonstrated that miRNAs can interact with the 5′-UTR of their target mRNAs and the DNA methylation machinery, thereby affecting chromatin status ( 11 ). (spandidos-publications.com)

When we looked at human tumor data from The Cancer Genome Atlas, we confirmed that this miRNA is elevated in CRC tumors relative to non-tumors," says Villanueva. (cornell.edu)

Potential inhibitory influence of miRNA 210 on regulatory T cells during epicutaneous chemical sensitization. (cdc.gov)

miRNA-21 may participate in tumorigenesis through interactions with lncRNA ( 12 ). (spandidos-publications.com)

Here, we aim to provide an overview on the mechanisms of miRNA biogenesis and to discuss the functional impact of miRNAs on PCa under radiation response. (frontiersin.org)
The scientists decided to take a closer look at this culprit miRNA, running functional evaluations in multiple cell models, and found that miR-24-3p promoted cancer cell survival. (cornell.edu)

At NEUmiRNA Therapeutics, we have successfully designed potent and selective RNA drugs for a novel disease target that enable unprecedented elimination of the drug resistance and chronic epilepsy in a preclinical model mimicking temporal lobe epilepsy ," Klitgaard told Medscape Medical News . (medscape.com)

Plasma lncRNA‑NEF and miRNA‑21 were negatively correlated in patients with osteosarcoma and healthy controls. (spandidos-publications.com)

Previous studies have already shown that abnormal activity of certain miRNAs can initiate and/or exacerbate disease phenotypes, including cancer. (cornell.edu)

In recent years, many studies have confirmed the involvement of miRNAs in biological processes of several types of cancer ( 4 , 9 , 10 ). (frontiersin.org)

This study indicates that CAFs-derived exosomal miRNA-130a may be a potential therapeutic target for cisplatin resistance in NSCLC. (dovepress.com)
We think that a miR-375-3p mimic could be a candidate therapeutic target," says Villanueva, "especially for patients with mutations that inhibit Tgf-β signaling. (cornell.edu)

Find that Viral miRNAs Mimic Human. (genengnews.com)
The researchers used genetically modified mouse organoids that mimic the cellular properties of the human intestine as models for colorectal cancer. (cornell.edu)
To do this, Villanueva and colleagues used genetically modified mouse organoids that mimic the cellular properties of the human intestine as models for colorectal cancer, which were provided by the Dow lab at Weill Cornell Medicine. (cornell.edu)
As an example, we analyzed a protemoics dataset (TMT10plex labelled) in which A431 cells (human epidermoid carcinoma cell line) were treated with three different miRNA mimics (Zhou Y. Et al Oncogene 2017). (bioconductor.org)
A human-mimic waterpipe smoking machine was used to replicate the puffing sequences of 31 human participants who completed two double-blind ad libitum smoking sessions in a controlled clinical setting: once with a tobacco-based product of their choosing and once with a flavor-matched tobacco-free product. (who.int)

MiRNAs 29a-3p and 34b-3p also resulted downregulated in vivo in a murine neuroblastoma progression model. (oncotarget.com)

A multitude of studies has demonstrated the role of miRNAs in chronic cardiovascular or renal disease processes [ 6 - 11 ]. (hindawi.com)

Had there not been (rather suspicious) weather problems in Dar Es Salaam, potentially Charla and Mirna could have reached the pit stop a full day and a half ahead of the last place team. (televisionaryblog.com)

More than 95% of testicular parenchymal abnormalities are identifiable on routine sonograms, but several other lesions commonly mimic testicular cancer. (medscape.com)

Downregulation of lncRNA‑NEF and upregulation of miRNA‑21 distinguished patients with osteosarcoma from healthy controls. (spandidos-publications.com)

The mechanisms of radioresistance are still poorly understood, despite it has been suggested that miRNAs play an important role in cell signaling pathways. (frontiersin.org)
In light of these discrepant data, MD Anderson's Anil Sood and colleagues sought to further investigate the potential mechanisms that underlie the miRNA family's different effects on different cancer types, he told Gene Silencing News . (genomeweb.com)

15 These studies strongly implicate the MIR137 locus in the susceptibility for schizophrenia and suggest that the encoded miRNA may be involved in the disorder through a role in shaping neurodevelopment. (nature.com)
To the best of our knowledge, no studies investigating the interaction between lncRNA-NEF and miRNAs have been reported. (spandidos-publications.com)

Synthetic miRNAs are easily transfected into many cell types for immediate gene knockdown. (abmgood.com)

Th)1 cells.5 Meanwhile, the function and immunity of with genetic predisposition, involving multiple cells, DCs are closely regulated by miRNAs. (cdc.gov)

Despite the recent surge about miRNA discoveries for cardiac I/R injury, there is still very little known about the mechanism details because of the complexity of cellular events and the interference of other risk factors. (hindawi.com)

The relationship between miRNAs and cancer was demonstrated for the first time in 2002, with miRNAs being stated as a potential mechanism that may contribute to improve some cancer therapeutic approaches through restoring or blocking the miRNAs function ( 11 ). (frontiersin.org)

Some specific miRNAs have been found to be frequently deregulated, and this deregulation has been associated with clinicopathological features of HCC, such as metastasis, recurrence and prognosis ( 7 - 9 ). (spandidos-publications.com)

A Treg suppression assay, including a miR-210 mimic, was utilized to investigate the suppressive ability of Tregs. (cdc.gov)

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