Mineralization chondrocytes. Medical search. Frequent questions

Tissue non-specific alkaline phosphatase (TNSALP) deficiency in osteoblasts and chondrocytes impairs bone mineralization, leading to rickets or osteomalacia. (wikipedia.org)
It dephosphorylates several substrates, including inorganic pyrophosphate (PPi), which inhibits bone mineralization produced by osteoblasts and chondrocytes. (medscape.com)
ENPP-1 , as ectonucleotide pyrophosphatase/ phosphodiesterase family member 1, is a membrane-bound glycoprotein, expressed on the outer surfaces of mineralizing cells, such as osteoblasts and chondrocytes. (biovendor.com)
2.8 cells recognize antigens, some of which are restricted to osteoblasts and chondrocytes. (docksci.com)
Immunohistochemical studies of antigen localization on cryostat sections of rat calvaria, long bone, and soft tissues demonstrated that five of these MAbs, UBIM 1,2,3,12, and 17, recognize antigens that are restricted to normal rat osteoblasts and chondrocytes. (docksci.com)

Hypertrophy of cartilage results from defective mineralization and decreased cartilage resorption, leading to a persistence of enlarged chondrocytes. (nih.gov)
This correlated with enhanced chondrogenesis and a reduction in the expression of markers associated with chondrocyte hypertrophy, as well as increased SMAD 2/3 nuclear localization in the encapsulated MSCs. (amrita.edu)
The inflammatory environment is correlated with extracellular matrix (ECM) degradation and chondrocyte hypertrophy in the development of osteoarthritis (OA). (oncotarget.com)
Wogonoside also inhibited hypertrophy and the generation of vascular endothelial growth factor (VEGF) in interleukin-1β (IL-1β)-induced chondrocytes. (oncotarget.com)
In conclusion, our stduy demonstrates that wogonoside attenuates IL-1β-induced ECM degradation and hypertrophy in mouse chondrocytes via suppressing the activation of NF-κB/HIF-2α by the PI3K/AKT pathway. (oncotarget.com)
Chondrocyte hypertrophy is an essential process required for endochondral bone formation. (silverchair.com)
Proper regulation of chondrocyte hypertrophy is also required in postnatal cartilage homeostasis. (silverchair.com)
Indian hedgehog (Ihh) and PTHrP signaling play crucial roles in regulating the onset of chondrocyte hypertrophy by forming a negative feedback loop, in which Ihh signaling regulates chondrocyte hypertrophy by controlling PTHrP expression. (silverchair.com)
To understand whether there is a PTHrP -independent role of Ihh signaling in regulating chondrocyte hypertrophy, we have both activated and inactivated Ihh signaling in the absence of PTHrP during endochondral skeletal development. (silverchair.com)
We found that upregulating Ihh signaling in the developing cartilage by treating PTHrP -/- limb explants with sonic hedgehog (Shh) protein in vitro, or overexpressing Ihh in the cartilage of PTHrP -/- embryos or inactivating patched 1( Ptch1 ), a negative regulator of hedgehog (Hh) signaling, accelerated chondrocyte hypertrophy in the PTHrP -/- embryos. (silverchair.com)
Conversely, when Hh signaling was blocked by cyclopamine or by removing Smoothened ( Smo ), a positive regulator of Hh signaling,chondrocyte hypertrophy was delayed in the PTHrP -/- embryo. (silverchair.com)
Furthermore, we show that upregulated Hh signaling in the postnatal cartilage led to accelerated chondrocyte hypertrophy during secondary ossification, which in turn caused reduction of joint cartilage. (silverchair.com)
Our results revealed a novel role of Ihh signaling in promoting chondrocyte hypertrophy independently of PTHrP , which is particularly important in postnatal cartilage development and homeostasis. (silverchair.com)
In addition, we found that bone morphogenetic protein (Bmp) and Wnt/β-catenin signaling in the cartilage may both mediate the effect of upregulated Ihh signaling in promoting chondrocyte hypertrophy. (silverchair.com)
Hypertrophic chondrocytes, a progeny of terminally differentiated chondrocytes from the proliferative zone, cease further proliferation, undergo hypertrophy in columns parallel to the axis of longitudinal elongation, and initiate the production of factors that trigger mineralization and vessel invasion. (pfmjournal.org)
Growth hormone (GH), sex hormone, thyroid hormone, as well as glucocorticoids affect chondrocyte proliferation and hypertrophy. (pfmjournal.org)
Truncation mutations cause early fusion of spinal vertebrae due to enhanced chondrocyte hypertrophy and early differentiation. (utsouthwestern.edu)

Prior studies of acute phosphate restriction during the endochondral phase of fracture healing showed delayed chondrocyte differentiation was mechanistically linked to decreased bone morphogenetic protein signaling. (researchgate.net)
The impairment of proliferation, differentiation, and mineralization in osteoblast is the main pathophysiological mechanism of osteoporosis, which has been proven by previous studies ( 4 , 5 ). (frontiersin.org)
Runx2 is a transcription factor essential for bone formation in mammals, affecting osteoblast and chondrocyte differentiation by regulating key genes crucial for bone and cartilage development. (bath.ac.uk)
Growth, metabolism, and differentiation of chondrocytes, which are the key cellular players in this process, are regulated by systemic hormones, local factors, and cellular signaling pathways. (pfmjournal.org)
Local factors, including transcription factors such as SRY-box 9 protein (SOX9), Runt-related transcription factor 2 (RUNX2), and bone morphogenetic proteins (BMPs), along with signaling pathways such as the Wnt pathway, play critical roles in chondrocyte proliferation and differentiation. (pfmjournal.org)
Chondrocyte growth, metabolism, and differentiation are complex processes. (pfmjournal.org)
Additionally, it was discovered that the chondrocytes in the epiphyseal growth plate underwent rapid terminal differentiation. (jotsrr.org)

In vitro, the mineralization ability of human rotator cuff cells from osteoarthritis donors was assessed by alizarin red or Von Kossa staining. (iasp-pain.org)
Chang, TDP-43 maintains chondrocyte homeostasis and alleviates cartilage degradation in osteoarthritis, Osteoarthr. (dntb.gov.ua)
Cherifi, Inhibition of sphingosine 1-phosphate protects mice against chondrocyte catabolism and osteoarthritis, Osteoarthr. (dntb.gov.ua)
Short description: The goal of this grant is to develop targeted nanoparticles to deliver drugs into chondrocytes of a mouse model of age-associated osteoarthritis. (uniroma2.it)

The proliferative zone, formed by cellular division originating from the resting zone, features flattened chondrocytes that undergo rapid replication, arrange longitudinally, and synthesize substantial amounts of extracellular matrix. (pfmjournal.org)

Elevated alkaline phosphatase levels are a hallmark of mineralization in both bone and cartilage, and appearance of type X collagen is correlated with the onset of mineralization in cartilage. (grantome.com)
They will also utilize chondrocyte transfection assays to test the hypothesis that high levels of alkaline phosphatase or type X collagen induce changes leading to mineralization. (grantome.com)
Rotator Cuff Tenocytes Differentiate into Hypertrophic Chondrocyte-Like Cells to Produce Calcium Deposits in an Alkaline Phosphatase-Dependent Manner. (iasp-pain.org)
Calcifications were amorphous areas surrounded by a fibrocartilaginous metaplasia containing hypertrophic chondrocyte-like cells that expressed tissue non-specific alkaline phosphatase (TNAP) and ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), which are two key enzymes of the mineralization process. (iasp-pain.org)
The ank gene product is one of several cell membrane proteins, including ectonucleoside triphosphate pyrophosphohydrolase enzymes and alkaline phosphatase, that regulate extracellular inorganic pyrophosphate levels and thereby regulate mineralization. (biomedcentral.com)
It also increases the synthesis of collagen, a key component of bone, and enhances the activity of alkaline phosphatase, an enzyme involved in bone mineralization. (jimcomunicaciones.com)

In addition, serial radiography studies suggest that defects in skeletal mineralization (i.e. rickets) persist and become more generalized. (wikipedia.org)
As the fetus accumulates 80% of its bone mineral content in the third gestational trimester ( 4 ), this time is critical to achieve normal skeletal mineralisation by 40 weeks gestation and support successful transition to post-natal life. (frontiersin.org)
PHENOTYPE: Mutations in this gene cause skeletal defects including runting, premature mineralization, and bone fusion. (utsouthwestern.edu)

The growth plate can be stratified into three distinct zones based on the size, morphology, orientation, proliferative potential, and function of chondrocytes: resting, proliferative, and hypertrophic zone. (pfmjournal.org)
Similarly, it may also improve the function of chondrocytes, which are cells that contribute to the formation of cartilage. (com.pk)

Studies suggest that the proliferation of osteoblasts, cells responsible for bone mineralization, may possibly be stimulated by PEG-MGF. (com.pk)

We provide evidence that tenocytes have a propensity to differentiate into hypertrophic chondrocyte-like cells to produce TNAP-dependent calcium deposits. (iasp-pain.org)
These condensed mesenchymal cells then differentiate into chondrocytes to form the cartilage anlagen. (silverchair.com)
In the chondroprogenitor region these young cells begin to differentiate into chondrocytes and to form the columnar structure characteristic of cartilage and bone. (avtoradio.tj)
MSCs have the ability to differentiate into adipocytes and chondrocytes forming fat tissue and cartilage as well as into bone cells - called osteocytes. (nature.com)

Nonetheless, this can be in sharp contrast with all the inherent stability of articular cartilage chondrocytes, in which these dynamic events must be restricted to assure life lengthy articular integrity and joint function. (rockinhibitor.com)
However, determined by the common embryology of cartilage and bone, as well as current proof MMP-12 Inhibitor Synonyms supporting distinct origins of growth plate and articular cartilage chondrocytes, it truly is not surprising that this hypothesis has been controversial (168). (rockinhibitor.com)

In vitro, tenocyte-like cells extracted from the rotator cuff were able to mineralize in osteogenic cultures, and expressed , , and , which are hypertrophic chondrocytes markers. (iasp-pain.org)
This, in turn, precipitates chondrocyte apoptosis, thereby, osteoblasts invade the hypertrophic zone and bone formation occurs [ 5 ]. (pfmjournal.org)
The mineralizing zone has hypertrophic chondrocytes similar to endochondral bone formation. (tamu.edu)
Type X collagen was in the pericellular matrix of hypertrophic chondrocytes and in some calcified matrix. (tamu.edu)

As a first step we attempted to induce cartilage mineralization in vitro, using micromass primary chondrocyte cultures, incubated with BMP2 and/or WISP1, which were examined histologically following a 3-week culture period. (iucc.ac.il)
Next, chondrocyte seeded collagen scaffolds were evaluated in vitro for expression profiles and ALP activity. (iucc.ac.il)
u003csup\u003e5\u003c\/sup\u003e Gelatin has shown, in vitro, to exert its action on chondrocytes by stimulating the production of type II collagen in cartilage. (healthmasters.com.au)

Many components have been implicated in inducing cells to undergo these mineralization-specific changes including matrix vesicle release, change in cellular redox status and appearance of mineral- ization-specific mRNAs and proteins. (grantome.com)
Regardless, an exploration on the mechanisms controlling alterations that chondrocytes undergo through their transition through the many stages of endochondral ossification could support to decipher these that underlie pathologic ossification in OA. (rockinhibitor.com)

We thus aimed to establish no matter whether an aberrant deployment of the transient chondrocyte phenotype is observed in STR/Ort mouse joints and regardless of whether this can be attributed to modified growth dynamics underpinned by an inherent endochondral development defect. (rockinhibitor.com)

Signaling mechanisms involved in the regulation of ePPi formation are poorly understood, but adenylyl cyclase activation decreases and protein kinase C activation increases the accumulation of ePPi in media surrounding cartilage or chondrocyte cultures [ 7 ]. (biomedcentral.com)
The regulation of chondrocyte function is mediated by several systemic, local, and cellular signaling pathways. (pfmjournal.org)
INTRODUCTION HE OSTEOBLAST (OB) is a key cell in bone formation, synthesizing and secreting the organic matrix of bone, and is involved in the regulation of matrix mineralization. (docksci.com)

This protein regulates bone mineralization by hydrolyzing extracellular nucleotide triphosphates to produce pyrophosphate (PPi) , which inhibits hydroxyapatite crystal deposition. (biovendor.com)
We discovered that calcipotriol inhibits chondrocyte GPX4-mediated ferroptosis by suppressing the expression of transforming growth factor-ß1. (bvsalud.org)

Chemical inhibition or specific gene silencing of this chaperone reduced the DNA condensation and fragmentation, typical of death by apoptosis, that is induced by NO donors in chondrocytes. (biomedcentral.com)
Chondrocyte metabolism is influenced by several cytokines and growth factors, which drive two qualitatively distinct functional programs in these cells: the catabolic program is induced by proinflammatory stimuli and characterized by the secretion of proteases, suppression of matrix synthesis, and induction of chondrocyte apoptosis. (biomedcentral.com)

Micromass cultures exhibited an osseous inducing trend following WISP1 administration, which was maintained in chondrocyte seeded scaffolds. (iucc.ac.il)

This peptide has been suggested to improve the migration of chondrocytes from the bone and, as a result, the maturation of these cells via studies conducted with mice. (com.pk)

Excess accumulation of ePPi has long been recognized as an important factor in the mineralization of cartilage with calcium pyrophosphate dihydrate (CPPD) crystals. (biomedcentral.com)
In addition to causing CPPD crystal formation, excess ePPi accumulation may also affect BCP mineralization. (biomedcentral.com)
We employed chemical inhibition and inductively coupled plasma mass spectrometry analyses to identify the effect of As on chondrocytes as well as studying its accumulation in organs after oral administration in mice. (bvsalud.org)

In this study, western blotting, quantitative reverse-transcription polymerase chain reaction, and immunofluorescence analysis were performed to elucidate the molecular mechanisms by which calcipotriol alleviates chondrocyte ferroptosis. (bvsalud.org)

Although the mechanisms of these effects are not known, the results suggest that local lead-related effects on growth-plate chondrogenesis and metaphyseal mineralization may be involved. (duke.edu)
Following this reasoning, we performed a differential proteomic analysis in order to search for OA-related changes in human articular chondrocyte intracellular proteins, which aimed to unravel those molecular mechanisms that participate in OA pathogenesis [ 4 ]. (biomedcentral.com)

We have shown that in cultured chondrocytes, ascorbic acid will also induce a transition from type II collagen to type X collagen. (grantome.com)
Moreover, wogonoside promoted the expression of anabolic factors Sox-9, type two collagen and aggrecan while inhibiting the expression of catabolic factors such as matrix metalloproteinases (MMPs) and thrombospondin motifs 5 (ADAMTS-5) in mouse chondrocytes. (oncotarget.com)
Finally, chondrocyte-seeded collagen scaffolds were implanted in a Lewis rats 8 mm critical calvaria defect model, which was imaged via live CT for 12 weeks until sacrifice. (iucc.ac.il)
Accordingly, in vivo analysis was carried out to assess the impact of WISP1-pretreated chondrocytes (WCS) versus untreated chondrocytes (UCS) in calvaria defect model and compared to untreated control comprised of a defect-associated blood clot (BC) or empty collagen scaffold (CS) implant. (iucc.ac.il)
These data support the theory that the gastrocnemius tendon has fibrocartilage characteristics and that type X collagen has a role in the tissue's mineralization. (tamu.edu)
It promotes the synthesis of collagen and other proteins necessary for bone formation, and also enhances the mineralization of bone tissue. (jimcomunicaciones.com)

A cell membrane protein, ANK, affects ePPi concentrations and the balance of mineralization in articular tissues. (biomedcentral.com)
The elaboration of ePPi by chondrocytes is a bioregulable process, enhanced by transforming growth factor β, ascorbate, retinoic acid, bone morphogenetic protein, transglutaminase, and thyroid hormones and diminished by parathyroid-hormone-related peptide isoforms, insulin-like growth factor-1, tumor necrosis factor α, and interleukin 1. (biomedcentral.com)
Our results demonstrate that the expression of SOX9, col2a1, and Aggrecan, as well as MMP13 and ADAMTS5 protein expression levels, decrease upon treatment with calcipotriol in interleukin-1ß stimulated chondrocytes. (bvsalud.org)
The role of this protein in chondrocytes is not well understood, although its increase in osteoarthritic cells has been reported. (biomedcentral.com)
Increased tendon calcification and a bone mineralization protein in musculoskeletal tissues with repetitive reaching task. (cdc.gov)

Aim 1 will test the hypothesis that osteoblast-fibroblast interactions can promote chondrocyte-mediated fibrocartilage formation on the scaffold. (grantome.com)
Especially, individuals afflicted with your dry out kind of AMD might be with the upper chances regarding building following dementia.The actual position of endogenous Bmp2 gene inside chondrocytes along with osteoblasts inside fracture CCCP solubility dmso healing ended up being researched through era and examination involving chondrocyte- along with osteoblast-specific Bmp2 depending knockout (cKO) these animals. (vegfr-3inhibitor.com)

These mice showed a phenotype of enhanced bone mineralization. (jotsrr.org)

Achondroplasia is caused by a mutation in the fibroblast growth factor receptor 3 ( FGFR3 ) gene that results in inhibited mineralization of chondrocytes (cartilage cells) in the growth plate (growing tissue near the ends of the long bones). (medscape.com)
In vivo, DMOG delivery significantly reduced mineralisation of the proteoglycan-rich cartilaginous tissue generated by MSCs within alginate hydrogels loaded with TGF-β3 and BMP-2. (amrita.edu)
Aim 3 will focus on the formation of four distinct yet continuous tissue regions (ligament, non-mineralized fibrocartilage, mineralized fibrocartilage, and bone) through the tri-culture of fibroblasts, chondrocytes and osteoblasts on the stratified scaffold. (grantome.com)
Chondrocytes implanted into critical cranial defect expedite the formation of native-like osseous tissue, especially after WISP1 priming in culture. (iucc.ac.il)
The chondrocyte is the only cell type present in mature cartilage and is responsible for repairing the cartilage tissue damaged by OA. (biomedcentral.com)
u003csup\u003e6\u003c\/sup\u003e Arthrex also contains silica to support bone, connective tissue and cartilage health plus boron for bone strength and vitamin K to regulate bone and cartilage mineralisation. (healthmasters.com.au)

We have generated a panel of 15 MAbs to ROS 17/2.8 cells, 5 of which recognize antigens restricted to normal rat OBs and chondrocytes in vivo. (docksci.com)

Porcine chondrocytes from aged donors make more ePPi than do chondrocytes from young donors [ 6 ]. (biomedcentral.com)

Ultimately, these data support the use of autologous chondrocytes to repair critical maxillofacial defects. (iucc.ac.il)

Available evidence indicates that it acts as a physiological inhibitor of mineralization. (bath.ac.uk)

Bone tissue's ability to regenerate effectively, maintain mineralization and repair itself after damage depends on its ability to dynamically remodel. (encyclopedia.pub)

Vosoritide, a biologic analog of C-type natriuretic peptide, prevents the inhibition of mineralization of chondrocytes caused by the FGFR3 mutation. (medscape.com)
Inhibition of Hsp90β by the chemicals Geldanamycin (GA) and Novobiocin (NB) caused a dose-dependent decrease of the NO production induced by IL-1β in chondrocytes, up to basal levels. (biomedcentral.com)

Among the proteins that were significantly altered in OA chondrocytes, we identified the β subunit of the chaperone Hsp90 as increased in diseased cells. (biomedcentral.com)

The primary goal of this research is to further our understanding of events controlling formation of calcified tissues by defining the factors in- fluencing matrix mineralization in cultured chondrocytes. (grantome.com)
Soft tissues were dissected out and frozen sectioned en bloc into 15 micrometer longitudinal sections, while bones were paraffin embedded and cut into 5 micrometer sections, before mounting on coated slides. (cdc.gov)

The recently published ank gene story establishes the direct tie between low ePPi levels and excess BCP mineralization of articular structures in another animal model. (biomedcentral.com)

Additionally, Indian hedgehog (Ihh) and C-type natriuretic peptide (CNP) are involved in the complex signaling network governing chondrocyte function. (pfmjournal.org)
Positive charge was introduced via cationization that additionally supported the stability of the scaffold in cell culture, and acted as nucleation point for mineralization during osteogenesis. (nature.com)

The resting zone, a source of stemlike progenitor cells that restores the reservoir of proliferative chondrocytes, is located farthest from the primary ossification center and assumes responsibility for preserving the architectural integrity of the growth plate [ 4 ]. (pfmjournal.org)

Factors leading to formation of mineralized matrix have been extensively studied in cultured osteoblasts and, to a lesser extent, in cultured chondrocytes. (grantome.com)

OA immunoreactivity increased in osteoclasts, periosteal cells, osteoctyes and osteoblasts, as well as in chondrocytes, tendon fi broblasts and mast cells, in 6-12 week HRLF rat radius and ulna. (cdc.gov)

The present results show how Hsp90β modulates NO production and NO-mediated cellular death in human OA chondrocytes. (biomedcentral.com)

Anatomy52

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