Mimetic studies of membrane proteins membrane protein. Medical search. Frequent questions

Widely-used membrane mimetics for protein purification and structure-function studies include detergents, lipid emulsions, nanodiscs, and amphipols. (the-scientist.com)
Even after careful optimization, detergents do not necessarily resemble the native lipid bilayer, and researchers do not always obtain stably solubilized membrane proteins with intact functions. (the-scientist.com)
Amphipols, nanodiscs, and and other complex membrane mimetics are more stabilizing than detergents, but their use demands additional purification steps, increasing protocol complexity. (the-scientist.com)
Moreover, mass photometry works with a variety of membrane proteins and mimetics including detergents, nanodiscs, styrene maleic acid copolymers form self-assembling lipid-protein particles (SMALPs), and amphipols, making it compatible with extant membrane protein purification workflows. (the-scientist.com)
Detergents and membrane mimetics can solubilize membrane proteins but do not always result in proper folding and functionality. (bvsalud.org)
As studying their native behavior is important, detergents and other membrane mimetics can be used for membrane protein solubilization prior to functional or structural studies. (refeyn.com)
Detergents are widely used for membrane protein extraction and throughout the protein purification process - detergents can also act as membrane mimetics for membrane protein solubilization. (refeyn.com)
In both cases, mass photometry supported the use of detergents for membrane protein extraction and solubilization. (refeyn.com)
Mass photometry provides valuable information on the purity and behavior of samples containing membrane proteins and membrane mimetics like detergents. (refeyn.com)
With the appropriate choice of detergents, membrane proteins with bound lipid species can be observed, which allows characterization of protein-lipid interactions. (ox.ac.uk)
Solubilizing membrane proteins with detergents to make them compatible with aqueous buffers and methodologies is usually one alternative but this is not always successful and even when successful questions can linger as to how detergents may alter protein structure6. (tech-strategy.org)
Other groups have also used HX MS for membrane proteins utilizing detergents [e.g. 15-17] and liposomes [e.g. (tech-strategy.org)
Detergents may pressure the protein into a nonnative conformation6 and can suppress peptide ionization if not properly removed prior to electrospray7 21 Creation of both liposomes and nanodiscs can be challenging. (tech-strategy.org)

Sodium dodecyl sulfate (SDS) micelles provide ideal mimetic media for high-resolution NMR studies of membrane proteins and proteins or peptides interacting with micellar aggregates. (ad-astra.ro)
It discusses the architecture of these viruses and fusion proteins and how small molecules, peptides, and antibodies can target these proteins successfully to treat and prevent disease. (caltech.edu)
The aim of the present study was to determine the secondary structures of the cementoin domain and to characterize the antibacterial properties of these peptides against P. aeruginosa . (biomedcentral.com)
We show here that the cementoin domain adopts an α-helical conformation both by circular dichroism and nuclear magnetic resonance analyses in the presence of membrane mimetics, a characteristic shared with a large number of linear polycationic antimicrobial peptides. (biomedcentral.com)
The N-terminal cementoin domain adopts α-helical secondary structures in a membrane mimetic environment, which is common in antimicrobial peptides. (biomedcentral.com)
However, unlike numerous linear polycationic antimicrobial peptides, membrane disruption does not appear to be the main function of either cementoin, elafin or full-length pre-elafin/trappin-2 against P. aeruginosa . (biomedcentral.com)
Hydrogen exchange (HX) mass spectrometry (MS) is dear for providing conformational details for protein/peptides that have become difficult to investigate with other strategies such as for example peripheral membrane protein and peptides that connect to membranes. (tech-strategy.org)

Because of this, membrane proteins are inherently unstable in aqueous solution, requiring distinct strategies for their solubilization, purification, and maintenance. (the-scientist.com)
However, like all membrane proteins, ssMPs are prone to misfolding and aggregation because of the hydrophobicity of transmembrane helices, making them difficult to study using common aqueous solution-based approaches. (bvsalud.org)
Energetics of protein side chain partitioning between aqueous solution and cellular membranes is of fundamental importance for correctly capturing the membrane binding and specific protein lipid interactions of peripheral membrane proteins. (uiuc.edu)
By preserving noncovalent interactions in the gas phase and using collisional activation to remove solubilization agents inside the mass spectrometer, native mass spectrometry (MS) is capable of studying isolated assemblies that would be insoluble in aqueous solution, such as membrane protein oligomers and protein-lipid complexes. (ox.ac.uk)

Approximately 25 percent of proteins localize to membranes, where they act as receptors and signaling molecules in biological processes, including respiration, neurotransmission, and molecular transport. (the-scientist.com)

Mass photometry offers a powerful solution for the characterization of membrane proteins. (the-scientist.com)
The major obstacle to structural characterization of membrane proteins is usually often the membrane itself which is generally not compatible with structural studies and many biophysical measurements. (tech-strategy.org)

Fibronectin type III domain-containing protein 5, the precursor of irisin, is a type I transmembrane glycoprotein that is encoded by the FNDC5 gene. (wikipedia.org)
Fibronectin domain-containing protein 5 is a membrane protein comprising a short cytoplasmic domain, a transmembrane segment, and an ectodomain consisting of a ~100 kDa fibronectin type III (FNIII) domain. (wikipedia.org)
Using this approach, we showed that apoptosis-inducing human transmembrane proteins, FasL and TRAIL, synthesized and displayed on oil drops induce apoptosis of cultured tumor cells. (bvsalud.org)
10. Mechanism of influenza A M2 transmembrane domain assembly in lipid membranes (2015) Sci Rep , 5, 11757. (ergttu.net)
Recent accounts indicate that ～58% of utilized drug targets were membrane proteins2 3 Despite much effort focused on both transmembrane and peripheral membrane proteins it has been difficult to obtain high resolution structural information for many membrane proteins. (tech-strategy.org)

1 Mass photometry is a bioanalytical characterization technology that can help researchers characterize membrane protein properties, such as oligomerization or other complex formations and assess the purity of samples containing membrane proteins. (the-scientist.com)
As measurements take less than 5 minutes and readily detect protein aggregation, it is easy to characterize membrane protein samples. (refeyn.com)

Starting with the early work on empty nanodiscs, we chart developments that culminate in recent high-resolution studies of membrane protein-lipid complexes ejected from nanodiscs. (ox.ac.uk)
We have previously applied hydrogen exchange (HX) mass spectrometry (MS) to membrane proteins7-14 primarily using liposomes or nanodiscs as the membrane mimetic. (tech-strategy.org)
22 23 for review) and protein directionality while nanodiscs have a background undesirable protein component (the membrane stabilizing protein) and lipid packing density is not easily altered. (tech-strategy.org)
As a result protein/peptide interactions with nanodiscs that require insertion or conformational changes may be restricted. (tech-strategy.org)

Earlier studies from Dr. Kaufmann's Anticancer Drug Action Lab provided some of the first biochemical evidence that conventional and targeted anti-cancer drugs induce programed cell death, also called apoptosis, in susceptible cells. (mayo.edu)
The BH3-mimetic Obatoclax induces apoptosis and decreases MCL1 expression in androgen-sensitive PCa cells, while castration-resistant PCa cells are less sensitive and react with an upregulation of MCL1 expression. (oncotarget.com)
In the present study, we observed that inactivation of the ERK1/2 signaling pathway using ABT-737 dramatically increased the expression of pro-apoptotic protein Bim via transcriptional and/or posttranslational regulation, in a cell type-dependent manner, inducing mitochondria-mediated apoptosis of human oral cancer cells. (oncotarget.com)
BH3-mimetics are a novel drug class of small molecule inhibitors of BCL2 family proteins which restore apoptosis in malignant cells. (biomedcentral.com)
In brief, apoptosis is triggered when "pro-death/damage sensing" BH3-only proteins activate the downstream effector molecules BAX and BAK, which dimerize on the mitochondrial outer membrane surface and permeabilize it, triggering the release of cytochrome C, caspase activation and committing the cell to programmed death. (biomedcentral.com)
BCL2 family proteins (BCL2, BCL-X L , MCL1, etc.) sequester BH3-only proteins and prevent apoptosis, and these anti-apoptotic properties are frequently upregulated in malignancy, making them an attractive therapeutic target [ 3 ]. (biomedcentral.com)
Released from the inhibitory action of BCL2 and related proteins, these pro-apoptotic BH3-only proteins are then able to activate BAX and BAK and initiate apoptosis (Fig. 1 ). (biomedcentral.com)

To preserve native membrane protein properties and structures, researchers typically use membrane mimetics to recreate a lipid environment in aqueous solutions. (the-scientist.com)
However, only a small number of structures of membrane proteins have been elucidated so far due to their low expression levels and the requirement of membrane mimetic environments for their study. (kent.ac.uk)
Here we revisit tau protein aggregation at primary, secondary, tertiary and quaternary structures. (ibecbarcelona.eu)
Membrane protein structures account for ～2.5% of all coordinate files deposited in the Protein Data Bank4 (www.rcsb.org5). (tech-strategy.org)

During his PhD, Jose studied the structure, folding properties and interactions of the SH3 domains of the CD2 adaptor protein using NMR as the main technique and was awarded an EMBO short-term fellowship to develop new NMR methodologies for the study of weak protein complexes in the laboratory of Dr Martin Blackledge at the Institut the Biologie Structurale (Grenoble, France). (kent.ac.uk)
This webinar shows the applications of mass photometry for the study of two examples of macromolecular complexes formed by eukaryotic membrane proteins. (refeyn.com)
The webinar showcases what mass photometry can do, and what makes it suitable for studies of membrane protein complexes. (refeyn.com)
Answer NMR spectroscopy has also benefited from detergent-solubilization in studying the high-resolution structure of full-length (FL) IMPs or truncated IMP constructs and in monitoring the conformational transitions in IMPs' monomers and complexes [103]. (lrrk2inhibitor.com)
Thus, the existing size limit for proteins and protein complexes studied by NMR in solution does not exceed 70 kDa even when advantageous pulse sequences are applied [10305]. (lrrk2inhibitor.com)

Jose joined the School of Biosciences in September 2016 as a Lecturer in Biological NMR and head of the NMR facility, where his research group specialises in the structural and functional studies of proteins able to insert in lipid membranes, forming pores and channels. (kent.ac.uk)
We study their mechanism of membrane insertion, the factors that trigger their activation and their inhibition using integrative structural biology tools, with a particular emphasis in solution NMR, as well as biophysics and fluorescence microscopy. (kent.ac.uk)
In addition, a brief overview is included of the techniques involved in structural biology and how it has informed the study of viruses. (caltech.edu)
For the interested reader, chapter 2 contains a review article that serves as a more in-depth introduction for both viruses as well as how the use of structural biology has informed the study of viral surface proteins and neutralizing antibody responses to them. (caltech.edu)
5. Structural basis for membrane anchoring and fusion regulation of the Herpes Simplex Virus fusogen gB (2018) Nat Struct Mol Biol , 25, 416-424. (ergttu.net)
Single-molecule tweezers, such as magnetic tweezers, are powerful mechanical manipulation tools that can probe nm-scale structural changes in a single membrane protein under force. (biorxiv.org)
The structure of non-structural protein 1 (NSP1) is composed of 180 amino acids. (indiansentinel.in)
This virus has sixteen non-structural proteins (NSP1-NSP16), of which, the NSP1 plays a vital role in the pathogenicity (ability to cause disease) of the virus. (indiansentinel.in)
The IIT Mandi team has experimentally studied the structural conformations of SARS-CoV-2 NSP1 under various conditions - in an organic solvent, membrane mimetic environment, and inside liposomes. (indiansentinel.in)

Chemical cross-linking provides detailed insights into these interactions suggesting a role for membrane fusion. (nature.com)
Zippering of the SNAREs is proposed to provide the required energy to initiate membrane fusion 6 , 7 and proceeds from the N-terminus towards the membrane-proximal C-terminus through interactions of their complementary SNARE motifs. (nature.com)
While the HMMM has been successfully applied to study spontaneous insertion of a number of peripheral proteins into membranes, a quantitative characterization of the energetics of membrane protein interactions in HMMM membranes has not been performed. (uiuc.edu)
As an additional measure of the ability of the HMMM model to describe membrane protein interactions, the water to membrane interface transfer energies were analyzed and found to be in agreement with the previously reported experimental and computational hydrophobicity scales. (uiuc.edu)
We discuss strengths and weaknesses of HMMM in describing protein membrane interactions as well as further development of model membranes. (uiuc.edu)
They do not necessarily replicate the native lipid bilayer environment, and only a small number of protein-lipid interactions can be resolved. (ox.ac.uk)
We anticipate that these developments will provide new insights into the factors that control dynamic protein-lipid interactions in a variety of tailored and natural lipid environments. (ox.ac.uk)
Using analytical techniques such as circular dichroism spectroscopy, fluorescence spectroscopy, and molecular dynamics simulations, the researchers have shown the dynamic changes in the conformation of the IDR of the NSP1, in response to its surroundings, due to hydrophobic and electrostatic interactions between the protein and the environment. (indiansentinel.in)
ROS may induce intracellular calcium increase and subsequent contraction of PASMCs via direct or indirect interactions with protein kinases, phospholipases, sarcoplasmic calcium channels, transient receptor potential channels, voltage-dependent potassium channels and L-type calcium channels, whose relevance may vary under different experimental conditions. (ersjournals.com)
Herein, we introduce a cardiac-mimetic cell-culture system that resembles the microenvironment in the heart and provides interactions with cardiomyocytes and electrical cues to the cultured fibroblasts for direct cardiac reprogramming. (thno.org)
Nano-thin and nano-porous membranes in the culture system facilitated interactions between fibroblasts and cardiomyocytes in coculture. (thno.org)

MLKL is a critical mediator of necroptosis that ruptures cellular membranes leading to cell death. (kent.ac.uk)
Objectives: To investigate the potential role of a commercially available preparation of micronized Amniotic Membrane Allograft (AMA) (Amniofix, MiMedx, USA) in the biological augmentation of an in vitro model of rotator cuff repair with respect to cellular pro- liferation, collagen content and mechanical properties of the bone- tendon interface. (researchgate.net)
Single-span membrane proteins (ssMPs) represent approximately one-half of all membrane proteins and play important roles in cellular communications. (bvsalud.org)
Keywords: Arf-1 deuterium melittin dynamics membrane Membrane proteins are involved in many cellular processes ranging from regulation recognition metabolism transport and signaling1. (tech-strategy.org)
Lipid packing density the number of lipid molecules per unit area is usually fluid in cellular membranes with some regions Org 27569 packed more tightly than other regions often dependent on the lipid composition degree of hydrocarbon chain saturation percentage of cholesterol and other factors24-27. (tech-strategy.org)
Recent studies have highlighted zinc's dynamic role as a "cellular second messenger" in the control of insulin signaling and glucose homeostasis. (hindawi.com)
Given the low plasma concentration of zinc and its importance in cellular signaling, it is essential that the availability and distribution of "free" zinc (free zinc is used to differentiate zinc involved in cell signaling from zinc that tightly bound to protein and therefore thermodynamically unavailable) are tightly controlled [ 6 ]. (hindawi.com)
Compared with individual exposures, co-exposure aerosols produced greater acellular and cellular oxidants detected by electron paramagnetic resonance (EPR) spectroscopy, and in vivo immune-spin trapping (IST), as well as synergistically increased lavage neutrophils, lavage proteins and inflammation related gene/protein expression. (cdc.gov)

A mass spectrometry study reported irisin levels ~3 ng/ml in human plasma, a level on par with other key human hormones, such as insulin. (wikipedia.org)
Interfacing Membrane Mimetics with Mass Spectrometry. (ox.ac.uk)
Mass spectrometry offers several sensitive approaches to study the biophysics of membrane proteins. (ox.ac.uk)

The first chapter of this thesis is an introduction to viruses with a specific focus on HIV-1 CD4 mimetic drugs and antibodies against SARS-CoV-2. (caltech.edu)
We are studying the mechanism of viral membrane fusion and its inhibition by drugs and antibodies. (stanford.edu)

Among the numerous available small molecule BH3 mimetics, ABT-737, a potent small molecule that binds to Bcl-2/Bcl-xL with high affinity, has anti-tumor activity in a wide variety of cancer cells. (oncotarget.com)
In contrast with oblimersen, BH3-mimetics are specific, potent and selective small molecule inhibitors of BCL2 family proteins, which disable their capacity to sequester "pro-death" BH3-only molecules. (biomedcentral.com)

We recently reported a highly mobile membrane mimetic (HMMM) model that accelerates lipid dynamics by modeling the membrane interior partially as a fluid organic solvent while retaining a literal description of the lipid head groups and the beginning of the tails. (uiuc.edu)

Here, we use cell-free protein synthesis in the presence of oil drops to create a one-pot system for the synthesis, assembly, and display of functional ssMPs. (bvsalud.org)
We further demonstrated the use of peptoid membranes as a robust platform to incorporate and pattern functional objects through large side-chain diversity and/or co-crystallization approaches. (nature.com)

All these features make peptoids highly attractive as synthetic analogues of lipids for self-assembly of membrane-mimetic 2D materials. (nature.com)
In the interfacial and headgroup regions of the membrane, the side chain free energy profiles show excellent agreement with profiles previously reported for conventional membranes with full tail lipids. (uiuc.edu)
For the latter, increasing collision energies allowed progressive removal of nanodisc components, beginning with the scaffold proteins and continuing through successive shells of lipids, allowing direct characterization of the stoichiometry of the annular lipid belt that surrounds the membrane protein. (ox.ac.uk)
Some proteins may alter the packing density of surrounding lipids perhaps as a result of conformational changes during function membrane insertion or other reasons. (tech-strategy.org)

Using mass photometry to count and measure the mass of the single membrane protein in solution allows researchers to gain valuable information on the purity and stability of their samples. (the-scientist.com)
Here, using the metal-free click chemistry of dibenzocyclooctyne (DBCO) cycloaddition and the rapid, strong binding of traptavidin to dual biotins (2xbiotin), we developed robust single-molecule tweezers that can perform thousands of force applications on a single membrane protein. (biorxiv.org)
Here, we describe a robust magnetic tweezer approach that can perform thousands of repetitive mechanical manipulations on a single membrane protein. (biorxiv.org)

Conventional methods use detergent to solubilize the protein prior to electrospray ionization. (ox.ac.uk)

Our work aims to elucidate how MLKL ruptures the membrane at molecular detail. (kent.ac.uk)
Maternal sera were dialyzed in vitro across individual or combined fetal membranes to elucidate the functions of the membranes in protein transport. (researchgate.net)
such an approach holds true for the COVID-19 virus as well and scientists across the globe are involved in studies to elucidate the structure and functions of these proteins to understand the viral disease and develop drugs that are effective against the virus. (indiansentinel.in)

Two of these family members, BAX and BAK, induce cell death by punching holes in the outer mitochondrial membrane, leading to the release of mitochondrial proteins to the cytoplasm, where they activate caspases. (mayo.edu)
Our current studies are designed to better understand the process of BAK activation and the mechanism by which this critical pro-apoptotic protein permeabilizes the outer mitochondrial membrane. (mayo.edu)

Thus, the proteins that transport zinc likely facilitate cell signaling processes that contribute to glycemic control in peripheral tissues by modulating cytosolic zinc concentrations. (hindawi.com)

Mass photometry provides the mass distribution of membrane proteins and other sample components at the single-molecule level within a few minutes using very little sample. (the-scientist.com)

It measures proteins' true molecular mass instead of relying on surrogate measures to predict molecular weights. (the-scientist.com)
HaloTag-chloroalkane, thiol-maleimide, etc ), at least one end of a protein or its tandem repeat is attached to a solid support without using long molecular spacers, such as DNA handles 25 - 32 . (biorxiv.org)
On the other hand, single-molecule tweezers using hundreds-of-nm long molecular handles flanking a protein of interest still rely on the conventional noncovalent tethers of the dig-antidig and biotin-streptavidin 10 - 15 , 19 . (biorxiv.org)
Both experimental and molecular dynamics simulation results show that peptoids assemble into membranes through an anisotropic formation process. (nature.com)
Due to their generally low expression levels and amphipathic nature, membrane proteins represent challenging molecular entities for biophysical study. (ox.ac.uk)
Data generated from laboratory-based studies are providing evidence that molecular regulatory processes may also contribute to the formation and rupture of aneurysms. (edu.au)

PrLDs-containing proteins have been involved in different organisms' regulatory processes. (ibecbarcelona.eu)

Zinc has "mimetic" activity where it is involved in a range of functions including insulin receptor signal transduction, insulin storage, secretion and tissues/organelle distribution, and inhibition of protein tyrosine phosphatases [ 5 - 8 ]. (hindawi.com)
GLP-1 has been reported to improve glucose-dependent insulin action through the G-protein-coupled receptor, GLP-1R (Drucker and Nauck, 2006). (eusaintlaurent.de)

The only currently approved BH3-mimetic, the selective BCL2 inhibitor venetoclax, is highly efficacious in chronic lymphocytic leukemia and has rapidly advanced to an approved standard of care in frontline and relapsed disease in combination with anti-CD20 monoclonal antibodies. (biomedcentral.com)
Venetoclax combination with standard regimens in follicular lymphoma, multiple myeloma and aggressive B cell neoplasms has shown some promise, but further studies are required to optimize dose and scheduling to mitigate increased myelosuppression and infection risk, and to find validated biomarkers of venetoclax sensitivity. (biomedcentral.com)

17. Membrane-bound alpha-Synuclein forms an extended helix: Long-distance pulsed ESR measurements using vesicles, bicelles and rod-like micelles (2008) J Am Chem Soc , 130, 12856-7. (ergttu.net)
To cut down the background and facilitate visualizing protein particles, absolutely free detergent micelles is often removed prior to the EM experiments [97]. (lrrk2inhibitor.com)
Given this, option NMR studies on IMPs call for detergent micelles to become as compact (little) as you can but still adequately mimic the membrane environment [103]. (lrrk2inhibitor.com)

In this case, the close proximity to the large-area surface and/or between the consecutively-connected proteins of the tandem repeat can hinder membrane protein studies since the lipid bilayer mimetics such as bicelles or vesicles of ∼100 nm or larger in diameter will not be properly accommodated in the narrow space 19 , 33 . (biorxiv.org)

Concentration of individual proteins in the dialyzates obtained, closely resembled the composition of amniotic fluid. (researchgate.net)
Our approach is to investigate individual proteins using cryoEM methods and to combine this information with cryoET reconstructions of the intact chemical synapse. (colorado.edu)

Understanding the conformational structure and associated functions of key viral proteins such as the NSP1 can eventually help develop therapeutics that can target these proteins and stop the virus in its tracks. (indiansentinel.in)

The LINE-1 retrotransposon protein, ORF1p, forms a condensate on RNA that is essential for retrotransposition, and may explain cis -preference through a co-translational assembly mechanism. (elifesciences.org)
Transcriptional activation of the orphan nuclear receptor Nurr1 by ligands targeting its heterodimer partner retinoid X receptor alpha (RXRα) occurs through a nonclassical pharmacological mechanism involving ligand-binding domain protein-protein interaction inhibition. (elifesciences.org)
The adaptor protein Grb2 is able to enhance the activity of the cytoplasmic tyrosine kinase Btk through a novel mechanism, revealing a new role for Grb2 in B-cell signaling. (elifesciences.org)

This thesis discusses two approaches to targeting two fusion proteins: Env from HIV-1 and S from SARS-CoV-2. (caltech.edu)
Polydispersity and heterogeneity of the membrane mimetic cassette is a critical issue for study by MS. Ever more complex data sets consisting of overlapping protein charge states and multiple lipid-bound entities have required development of new computational, theoretical, and experimental approaches to interpret both mass and ion mobility spectra. (ox.ac.uk)

Fusion of host and viral membranes is critical for the lifecycle of enveloped viruses. (caltech.edu)
Studying viral fusion proteins can allow us to better understand how they shape immune responses and inform the design of therapeutics such as drugs, monoclonal antibodies, and vaccines. (caltech.edu)

The sequence of the protein includes a signal peptide, a single fibronectin type III domain, and a C-terminal hydrophobic domain that is anchored in the cell membrane. (wikipedia.org)
A protein initiated at Met-76 would be missing the signal peptide and would be trapped in the cytoplasm. (wikipedia.org)
We validated that known conformational changes in the presence of a monolayer/membrane could be Org 27569 observed with the peptide melittin and the myristoylated protein Arf-1. (tech-strategy.org)
To study membrane protein/peptide conformational changes therefore control over lipid packing density can sometimes be critical28 including the ability to reproducibly produce measure and change lipid packing density. (tech-strategy.org)

Earlier in 2020, we have shown through bioinformatics studies that NSP1 C-terminal region has intrinsic disorder propensity between 0.4 to 0.5 scales, i.e. very close to borderline of intrinsic disorder prediction. (indiansentinel.in)

12. Tau binds to membrane surfaces via short amphipathic helices located in its microtubule-binding repeats (2014) Biophys J , 107, 1441-52. (ergttu.net)

The aim of this study was to evaluate the sealing capability and tissue interaction of mussel-mimetic tissue adhesive (mussel glue) in comparison to fibrin glue on punctured fetal membranes in vivo. (researchgate.net)
Single-molecule tweezers are an emerging tool for membrane protein studies, which has yielded important insights into membrane protein biogenesis, interaction with drugs, and chaperone effects on their folding 1 - 3 . (biorxiv.org)

Partitioning of amino acids into a model membrane: Capturing the interface. (uiuc.edu)
In addition to its role in glucose metabolism, this pathway also regulates the redirection of free amino acids to protein synthesis via the mTOR-signaling pathway. (hindawi.com)

By quantifying the mass distribution of biomolecules in their samples, researchers gain valuable insights into sample purity, heterogeneity, and protein stoichiometry. (the-scientist.com)

Building on these earlier results, current studies in our lab focus on understanding the pathways that lead to caspase activation and the mechanisms that regulate those pathways. (mayo.edu)
Combined with the other studies in the Anticancer Drug Action Lab, our studies of apoptotic pathways are providing new insight into factors that govern cancer cell sensitivity to targeted anti-cancer agents and immunotherapy, while also elucidating the mechanisms of resistance to a variety of novel anti-cancer agents. (mayo.edu)

Our studies suggest that oil drops prevent aggregation of some in vitro-synthesized ssMPs by allowing these ssMPs to localize on oil surfaces. (bvsalud.org)

To achieve this, the neuronal SNARE (i.e., soluble N -ethylmaleimide-sensitive factor attachment protein receptor) complex brings the vesicle and presynaptic membranes in close proximity, thereby, mediating the fusion of the two membranes resulting in exocytosis of neurotransmitters. (nature.com)
In this work, simulations have been performed on the receptor binding domain of SARS-CoV-2 spike glycoprotein to study what residues are more susceptible to be attacked by ·OH, which is one of the most reactive radicals associated to oxidative stress. (ibecbarcelona.eu)
In addition, our recent studies have implicated the death receptor pathway in the TP53-independent anti-leukemic effects of inhibitors of the kinases Chk1, ATR and Wee1 as single agents. (mayo.edu)

A 2021 review highlights new discoveries of irisin in brain function and bone remodeling, but criticizes all studies using commercial antibody assays to measure irisin concentrations. (wikipedia.org)

This pathway is regulated by a family of proteins called BCL2 family members that monitor the intracellular environment and regulate the integrity of mitochondria. (mayo.edu)

The studies also showed that selective protein degradation occurs during this process and that intracellular cysteine proteases called caspases contribute to this degradation. (mayo.edu)

For liposomes there can be issues with reproducibility lipid membrane curvature effects (especially in vesicles see Refs. (tech-strategy.org)

Additionally, selecting the best detergent to obtain large quantities of active, homogeneous, and stable protein requires laborious trial and error. (the-scientist.com)
You will also learn how to perform quick dilutions prior to measurement to minimize detergent noise in your samples after membrane protein extraction. (refeyn.com)
It might be hard to distinguish the protein particle from a detergent by means of a damaging EM stain, as identified in the study of citrate transporter CitS in DDM and DM [96]. (lrrk2inhibitor.com)
These studies had been performed exclusively or partly on detergent-solubilized IMPs. (lrrk2inhibitor.com)
Gas-phase activation inside the mass spectrometer removes the detergent to yield the isolated proteins with bound ligands. (ox.ac.uk)

The first part in chapter 3 involves a study on conformational changes induced in the HIV-1 Env protein by CD4-mimemtic drugs using single particle cryo-EM. (caltech.edu)

It mediates fusion of synaptic vesicles with the presynaptic plasma membrane resulting in exocytosis of neurotransmitters. (nature.com)
Fusion of synaptic vesicles with the presynaptic membrane occurs in the presence of the NSF (N-ethylmaleimide-sensitive-factor) / α-SNAP (α-soluble NSF attachment protein) disassembly machinery. (nature.com)

Mass photometry advances membrane protein characterization by offering fast and precise mass measurements of samples containing membrane mimetics. (the-scientist.com)

Using this method, researchers can directly analyze samples without labeling or other modifications necessary for traditional methods, which can interfere with a membrane protein's structure, function, and activity. (the-scientist.com)
We are also developing methods to identify ligands for non-druggable protein surfaces. (stanford.edu)
Analytical methods that make use of membrane mimetics (i.e. artificial membranes) are attractive alternatives because the structure of the membrane protein is usually more likely to be preserved in the mimetic. (tech-strategy.org)

We report here the free energy profiles for partitioning of 10 protein side chain analogues into a HMMM membrane. (uiuc.edu)

Integral membrane proteins and membrane-associated proteins are essential players in biological processes. (kent.ac.uk)
Membrane proteins fulfill vital biological roles, representing a significant portion of potential therapeutical targets for a variety of diseases. (refeyn.com)
By applying up to 50 pN for each cycle, which is sufficiently high for most biological processes, we were able to observe repetitive forced unfolding for a designer membrane protein up to approximately 1000 times on average. (biorxiv.org)

Shortly afterwards, a study using FNDC5 knock-out mice as well as artificial elevation of circulating irisin levels showed that irisin confers beneficial cognitive effects of physical exercise and that it can serve an exercise mimetic in mice. (wikipedia.org)

Yet, performing structure-function analyses on membrane proteins is challenging because in their native states they are embedded within the plasma membrane's hydrophobic lipid bilayer. (the-scientist.com)

Our lab is interested in proteins with the ability to exists in a soluble form and in a membrane-bound form, where they usually form channels of pores. (kent.ac.uk)
It also shows data comparing soluble and aggregated protein samples to demonstrate how mass photometry can quickly evaluate membrane protein solubilization. (refeyn.com)

Membrane proteins play critical physiological roles and make up the majority of drug targets. (ox.ac.uk)

Platelets play a primary role in this process, interacting with subendothelium-bound von Willebrand factor (vWf) via the membrane glycoprotein (GP) Ib complex. (medscape.com)

It is challenging for researchers to assess the quality of mimetics and identify which samples contain highly concentrated, stable membrane proteins. (the-scientist.com)
19. High-field/high-frequency EPR study on stable free radicals formed in sucrose by gamma-irradiation (2006) Free Radical Res , 40, 553-563. (ergttu.net)
An ability to develop sequence-defined synthetic polymers that both mimic lipid amphiphilicity for self-assembly of highly stable membrane-mimetic 2D nanomaterials and exhibit protein-like functionality would revolutionize the development of biomimetic membranes. (nature.com)
Here we report the assembly of lipid-like peptoids into highly stable, crystalline, free-standing and self-repairing membrane-mimetic 2D nanomaterials through a facile crystallization process. (nature.com)
Herein we report the first example of self-assembling highly stable and self-repairing membrane-mimetic 2D nanomaterials by designing lipid-like sequence-defined peptoids. (nature.com)

Anatomy45

Organisms13

Chemicals and Drugs108

Analytical, Diagnostic and Therapeutic Techniques and Equipment31

Phenomena and Processes58

Disciplines and Occupations2

Technology, Industry, Agriculture7

Information Science3

Health Care1

Detergents13

Peptides7

Aqueous solution4

Signaling molecules1

Characterization of membrane proteins2

Transmembrane5

Characterize membrane protein2

Nanodiscs4

Apoptosis7

Structures4

Complexes5

Structural9

Interactions11

Cellular8

Mass spectrometry3

Drugs and antibodies2

Small molecule2

Organic solvent1

Functional2

Lipids4

Single membrane protein3

Solubilize1

Elucidate3

Outer mitochon2

Peripheral1

Photometry provides1

Molecular6

Regulatory1

Insulin2

Venetoclax2

Micelles3

Bicelles1

Individual proteins2

Target these proteins1

Mechanism3

Approaches2

Viral2

Peptide4

20201

Amphipathic1

Interaction2

Amino acids2

Heterogeneity1

Mechanisms2

Aggregation1

Receptor3

20211

BCL21

Selective1

Liposomes1

Detergent5

Conformational changes1

Vesicles2

Advances1

Methods3

Analogues1

Biological3

Artificial1

Analyses1

Soluble2

Roles1

Glycoprotein1

Stable5