• Malena and Klepetan's love affair is one example of the role tagging storks plays in the environmental research and conservation of migratory birds. (iflscience.com)
  • These migratory birds are the largest in North American, standing at 5ft tall, and have been an endangered species since 1967. (spidertracks.com)
  • Click on Hunting, then Migratory Birds, to find your way to the reports…filed each Friday. (blogspot.com)
  • at steak are shore birds and migratory birds. (archive.org)
  • Others include birds' immunity and migratory patterns. (medscape.com)
  • Alkhurma Hemorrhagic Fever Virus RNA in Hyalomma rufipes Ticks Infesting Migratory Birds, Europe and Asia Minor. (cdc.gov)
  • In Guinea-Bissau and some other African countries, natural parks were host to various species of migratory birds. (who.int)
  • The epithelial-mesenchymal transition (EMT) is an example of cellular plasticity, where an epithelial cell acquires a mesenchymal-like phenotype that increases its migratory and invasive properties. (mdpi.com)
  • Additionally, the effect of the targeted liposomal combinations was evaluated on the percentage of ALDH+/high putative triple-negative breast CSC population and phenotype, including mammosphere-forming capacity and live single-cell tracking. (uc.pt)
  • Furthermore, bulk-RNAseq datasets further confirm this phenotype by the upregulation of promotility cell migration-related gene pathways in the enriched phenotypic exhausted T cell tumours. (preprintclub.com)
  • We performed two-photon time lapse microscopy in GFP-expressing transgenic zebra finches to visualize migratory dynamics of cell populations in vivo. (cosyne2023neurodev.com)
  • The profile of genetic activity and the tissue contribution by cells in the organizer change during gastrulation, suggesting that the organizer may be populated by a succession of cell populations with different fates. (silverchair.com)
  • Indeed, the behavior of almost all immune cell populations in the bloodstream is altered in some way during and after exercise ( 7 , 8 ). (frontiersin.org)
  • Additionally, tumor microenvironment is constituted by different cellular populations, including cancer cells, cancer stem cells (CSCs), endothelial cells, immune inflammatory cells and cancer-associated fibroblasts, that contribute to the tumor pathogenesis. (uc.pt)
  • Furthermore, the identification of common markers among different tumor cell populations could increase efficacy of new anticancer therapeutic strategies. (uc.pt)
  • Therefore, nucleolin emerged as a potential common marker for different breast tumor cell populations due to the demonstration of its overexpression on the cell surface of breast CSCs, non-stem breast cancer cells and endothelial cells from the tumor blood vessels. (uc.pt)
  • A robust strategic plan was developed for 2010-2012 encompassing district-specific plans and focused strategy on security compromised areas, performance-based payment, independent monitoring, attention to migratory populations, social mobilization, and strategic cooperation with Afghanistan. (who.int)
  • While mesenchymal and epithelial cells are dominated by adhesive interactions the amoeboid crawling of leukocytes is largely controlled by guidance cues of the chemokine family 3 , 4 . (nature.com)
  • Subsequently, neural crest cells from the roof plate of the neural tube undergo an epithelial to mesenchymal transition, delaminating from the neuroepithelium and migrating through the periphery where they differentiate into varied cell types. (wikipedia.org)
  • Triple negative breast cancer cells also gain survival advantages by producing a protein called E-cadherin, typically found in epithelial cells that line the ducts and coverings of organs and frequently renew themselves. (sciencedaily.com)
  • When triple negative breast cancer cells gain such survival and migratory qualities, scientists classify their cellular state as so-called hybrid epithelial mesenchymal (EMT) cells. (sciencedaily.com)
  • RGs are epithelial-like cells that extend a long fiber from the VZ to the basal lamina overlying the CP. (jneurosci.org)
  • Initial works revealed that activation of the EMT program in epithelial cells induces the acquisition of stem cell properties, which in the context of cancer may contribute to the appearance of tumor initiating cells (TIC). (mdpi.com)
  • Herein, we develop a label-free, automated algorithm to identify and track individual epithelial cells within a free-moving cluster. (omicsdi.org)
  • We use this algorithm to analyze the effects of partial E-cadherin knockdown on collective migration of MCF-10A breast epithelial cells directed by an electric field. (omicsdi.org)
  • Here, we analyze the combined effect of intercellular interactions and electric fields on the directional migration of non-transformed mammary epithelial cells, MCF-10A. (omicsdi.org)
  • Benzyl isothiocyanate inhibits epithelial-mesenchymal transition in cultured and xenografted human breast cancer cells. (omicsdi.org)
  • were used in the development of a human immunocompetent Organ-on-Chip - a human cell-based in vitro model of the choroid layer of the eye integrating melanocytes and microvascular endothelial cells, covered by a layer of retinal pigmented epithelial cells. (ilexlife.com)
  • The thyroid gland forms as a proliferation of endodermal epithelial cells on the median surface of the developing pharyngeal floor. (medscape.com)
  • Neural crest cells are a temporary group of cells that arise from the embryonic ectoderm germ layer, and in turn give rise to a diverse cell lineage-including melanocytes, craniofacial cartilage and bone, smooth muscle, peripheral and enteric neurons and glia. (wikipedia.org)
  • All pyramidal neurons descend from cell divisions of radial glial cells (RGs) in the VZ, typically through various types of basal (or intermediate) progenitors in the SVZ ( Florio and Huttner, 2014 ). (jneurosci.org)
  • Organotypic slices of ferret neocortex were infected with a GFP-adeno-associated virus that preferentially targets postmitotic cells, thus robustly labeling migrating neurons. (jneurosci.org)
  • Live imaging was then performed and migration of individual neurons was tracked over time. (jneurosci.org)
  • Cell-type identity was assessed after imaging by immunostaining for Satb2, a marker of upper-layer neurons. (jneurosci.org)
  • Migratory neurons dispersed in all directions and made frequent course changes, a pattern observed across male and females, juveniles and adults. (cosyne2023neurodev.com)
  • By performing tetrode recordings in a restrained preparation, we found CX neurons that encode the animal's orientation using external and internal sensory cues, similarly to mammalian head direction cells as well as the direction of recent rotations (Varga and Ritzmann, 2016). (jhu.edu)
  • This study was designed to deepen the knowledge of the role played notably by CAFs in promoting breast tumor cell migratory skill through the analysis of the expression/activity of downstream potential target molecules and of the contribution of paracrine signalings. (fupress.net)
  • To do this, breast cancer cells gain a cellular skeleton protein dubbed vimentin, which enhances the migratory ability of so-called mesenchymal cells, a cell type typically found in bones and bone marrow that moves around and makes new cells. (sciencedaily.com)
  • Human bone marrow derived mesenchymal stromal cells (hMSC) is an excellent source, as these cells can be genetically modified to express proteins/drugs and also have an exceptional migratory or homing ability towards tumors, injury or inflammation. (brainsciencefoundation.org)
  • This project will study the use genetically modified human mesenchymal stromal cells as a local therapeutic drug delivery vehicle for the targeted delivery and secretion of chemotherapeutic/biologic agents for treatment of brain tumors. (brainsciencefoundation.org)
  • The results at the conclusion of this project will provide us all the information necessary for a pilot phase I feasibility and safety study for treating brain tumor patients with mesenchymal stem cells that have been engineered to express cytosine deaminase (MSC-CD) followed by 5-FC therapy. (brainsciencefoundation.org)
  • Mesenchymal stem cells (MSCs) are bone marrow-derived adult stem cells that are involved in wound healing and tissue regeneration. (gatech.edu)
  • Mesenchymal stem cells (MSCs) that display homing and infiltration properties towards tumor cells are a promising cellular targeting vector for brain tumor therapy but are limited to local-regional delivery in current preclinical models. (tmu.edu.tw)
  • Exploring the interactions between normal and malignant immune cells and these vascular scaffolds may thus reveal multiple points of intervention to treat CNS invasive processes. (medicilon.com)
  • After gastrulation, neural crest cells are specified at the border of the neural plate and the non-neural ectoderm. (wikipedia.org)
  • The quail-chick marking system, devised by Nicole Le Douarin in 1969, was another instrumental technique used to track neural crest cells. (wikipedia.org)
  • Chimeras, generated through transplantation, enabled researchers to distinguish neural crest cells of one species from the surrounding tissue of another species. (wikipedia.org)
  • With this technique, generations of scientists were able to reliably mark and study the ontogeny of neural crest cells. (wikipedia.org)
  • A molecular cascade of events is involved in establishing the migratory and multipotent characteristics of neural crest cells. (wikipedia.org)
  • Neither scientist set out to become an expert on neural crest cells, as these inveterate migrants are known. (stowers.org)
  • Now, after two decades of graduate school and postdoctoral training between them, both are at the Stowers Institute for Medical Research studying neural crest cells, albeit from vantage points that couldn't be more different. (stowers.org)
  • Kulesa, the director of Imaging, develops sophisticated imaging technology to understand how neural crest cells travel long distances and assemble various structures, such as the peripheral nervous system. (stowers.org)
  • Using mouse, chick or zebrafish models, both test what goes awry when mutations derail migrating neural crest cells in a developing embryo. (stowers.org)
  • Neural crest cells spring from the crest or dorsal ridge of the embryonic brain and spinal cord and then migrate to faraway regions of the face, heart or gut. (stowers.org)
  • Failure of neural crest cells to either complete their journey or mature on arrival causes widely varying birth defects known collectively as neurocristopathies. (stowers.org)
  • The group then analyzed mouse embryos using molecular markers and discovered that precursors of neural crest cells in the brain and spinal cord began dying even before crest cells destined to help build the face could start migrating. (stowers.org)
  • In 2008, Trainor's group reported in Nature Medicine that blocking a gene that promotes cell death, called p53 , allowed nascent neural crest cells in Tcof1 -mutant mice to survive, preventing manifestation of the animals' craniofacial defects. (stowers.org)
  • The rudimentary lateral thyroid develops from neural crest cells, while the median thyroid, which forms the bulk of the gland, arises from the primitive pharynx. (medscape.com)
  • Despite evidence suggesting that in vivo chemokines are often immobilized to sugar residues, limited information is available how cells respond to immobilized chemokines. (nature.com)
  • Coincubation with Leishmania changes the migratory pattern of dendritic cells in vivo . (biomedcentral.com)
  • hMSC is an ideal candidate and compatible for clinical use as these cells can be harvested by bone marrow aspiration from individual patients without difficulty, processed ex vivo very efficiently, and later transplanted back into the same patients during surgery thereby reducing immunological consequences. (brainsciencefoundation.org)
  • We have tested the functionality of RQR8 in vitro and in vivo as well as in combination with T-cell engineering components. (ashpublications.org)
  • To track Chlamydia-specific CD4 T cells in vivo, we recently developed several new tools called Chlamydia-specific MHC Class II tetramers. (uams.edu)
  • This highlights the relevance of studying the dynamic T cell behaviour in vivo. (preprintclub.com)
  • They developed two-photon-based imaging allowing the study of endogenous human T cells behaviour in vivo. (preprintclub.com)
  • Therefore, it is more accurate to the real T cell migration in vivo. (preprintclub.com)
  • The process of gastrulation is linked to determination of mesodermal cell fates, such that patterning of tissue fates and patterning of cell behavior are interconnected. (nature.com)
  • This therapeutic strategy employs genetic modification of hMSC and exploits the tumor tropic behavior, for tracking to sites of tumor cell invasion and allows on-site generation of drug to control tumor growth. (brainsciencefoundation.org)
  • The scientists found that when triple negative breast cancer cells invade other tissues on their way to another part of the body, they gain two cellular properties: better movement and survival. (sciencedaily.com)
  • The furry ( fry ) gene encodes an evolutionarily conserved protein with a wide variety of cellular functions, including cell polarization and morphogenesis in invertebrates. (nature.com)
  • The 5-FU produced locally will diffuse through cellular membranes thereby increasing the cytotoxicity to neighboring tumor cells without increasing the risk of harmful systemic effects. (brainsciencefoundation.org)
  • Here, we investigated whether placenta-derived MSCs (P-MSCs) are a superior cellular vector for systemic targeting of glioblastoma stem-like cells (GSCs), with an imaging modality to real-time monitor the trafficking P-MSCs to glioblastoma sites. (tmu.edu.tw)
  • Polyethylene glycol-coated superparamagnetic iron oxide (PEG-SPIO) was synthesized for cellular labelling and imaging P-MSCs, displaying high cellular uptake and no cytotoxic effect on P-MSCs cell proliferation or stemness property. (tmu.edu.tw)
  • This study suggests the possibility of innovative systemic P-MSC-based cell therapy for aggressive GSCs, developing a state-of-the-art theranostic technique for real-time tracking of therapeutic P-MSCs tumor infiltration through cellular MRI. (tmu.edu.tw)
  • Moreover, the plasticity associated with CSCs and non-stem cancer cells, which interconvert according to environmental stimuli, illustrates the cellular dynamics within the tumor microenvironment and contributes to drug resistance and tumor progression. (uc.pt)
  • We are currently employing a variety of immunological methods, including flow cytometry and immunofluorescence imaging, in conjunction with cellular approaches and gene ablation mouse models to characterize the memory CD4 T cell responses in the FRT during Chlamydia reinfection. (uams.edu)
  • However, the lack of dynamic study on the migratory behaviour, and cellular interactions of this particular population limits our understanding of this state and its potential outcome. (preprintclub.com)
  • Newer tracking systems are able to forgo satellite monitoring and instead rely on cellular networks, which can handle more data and provide a critter's coordinates at more frequent intervals. (nature.org)
  • Methods We established new patient-derived PA cell lines that preserve molecular features of the primary tumors and can be studied in OIS and proliferation depending on expression or repression of the SV40 large T antigen. (ibecbarcelona.eu)
  • Studying a deadly type of breast cancer called triple negative, scientists say they have identified key molecular differences between cancer cells that cling to an initial tumor and those that venture off to form distant tumors. (sciencedaily.com)
  • More specifically, Ewald and Johns Hopkins postdoctoral fellow Eloïse Grasset, Ph.D., and others on the research team found several unique properties in the cells of mice engineered to have the mouse-version of triple negative breast cancers, and mice implanted with tumors from people with triple negative breast cancer. (sciencedaily.com)
  • Stem cell-based therapies provide a promising approach for treatment of tumors in humans. (brainsciencefoundation.org)
  • TSPO is overexpressed in primary brain tumors and has been shown to correlate with the malignancy of the tumor cells. (uni-regensburg.de)
  • The invention is a method for drawing malignant cells out of tumors using structures such as nanofibers, chemical gradients that cancer cells migrate towards and biophysical cues that promote tumor cell migration. (freedomfromcancerchallenge.org)
  • Cancer cells can be collected from tumors (surgical biopsies) or from the blood (so-called liquid biopsies). (freedomfromcancerchallenge.org)
  • Non-surgical methods of obtaining cancer cells from tumors have not been previously described. (freedomfromcancerchallenge.org)
  • Differential migratory patterns illustrate the potential of our setup to quantitatively study the competitive response to both types of gradients. (nature.com)
  • To look more closely at molecules involved in hybrid EMT states, the scientists sought the help of Elana Fertig, Ph.D., division director and associate director of quantitative sciences and co-director of the Convergence Institute at the Johns Hopkins Kimmel Cancer Center, to track the molecular patterns of individual cells in cell assays that model invasion out of the primary tumor and formation of a colony in a metastatic site. (sciencedaily.com)
  • We present a new bioreactor that harnesses electrotaxis-directed cell migration along electric field gradients-by integrating four independent electrodes under computer control to dynamically program electric field patterns, and hence steer cell migration. (omicsdi.org)
  • One of the ways scientists track infected mosquito patterns in California is by using flocks of strategically placed sentinel chickens. (medscape.com)
  • A re-evaluation of the role of B cells in protective immunity to Chlamydia infection. (uams.edu)
  • The scientists found that most of the metastatic cells morph into the more mobile, better surviving, hybrid EMT state. (sciencedaily.com)
  • Scientists have uncovered how ALL cells invade the CNS, a relatively common occurrence that makes the cancer immeasurably more difficult to treat. (medicilon.com)
  • Due to practical constraints of flying in an aircraft, the scientists tracked moths continuously until the insects stopped on route. (eurekalert.org)
  • In one recent case, LTIB scientists working with Gulley saw potential for a therapeutic vaccine that targets Brachyury, a molecule that is overexpressed in cancer cells but not in normal tissue. (nih.gov)
  • Working with researchers at the University of California, Davis, TNC scientists used radio collars, camera traps and other tracking methods to monitor mountain lions like this one in Southern California from 2001 to 2017. (nature.org)
  • As a proof of principle we study the response of dendritic cells to their major guidance cues, chemokines. (nature.com)
  • We tracked migration of dendritic cells towards immobilized gradients of the chemokine CCL21 and varying superimposed soluble gradients of CCL19. (nature.com)
  • The best understood example for the significance of immobilized vs. soluble chemokine gradients is the trafficking of dendritic cells (DCs). (nature.com)
  • These cells include dermal resident dendritic cells (DC), epidermal Langerhans cells, neutrophils and monocytes, which are released from the blood when the dermal capillary is disrupted and a reputed blood lake is formed [ 3 - 6 ]. (biomedcentral.com)
  • These protocols can be adapted to study skin-lymphoid tissue migration of dendritic cells, inflammatory monocytes, neutrophils, and other phagocytic myeloid cells in response to vaccine antigens and infection. (bvsalud.org)
  • MSCs are good candidates for the development of cell-based therapeutics. (gatech.edu)
  • Together these techniques will be used to identify the mechanical and adhesive properties of migratory MSCs. (gatech.edu)
  • Results demonstrated that P-MSCs had greater migratory activity towards GSCs and across blood-brain barrier compared with bone marrow-derived MSCs, and this activity was enhanced by hypoxia precondition. (tmu.edu.tw)
  • Cell labeling techniques advanced the field of neural crest because they allowed researchers to visualize the migration of the tissue throughout the developing embryos. (wikipedia.org)
  • This may enable an individual to visualize the monitors from the descended cells and their ancestor within an interactive multigeneration story. (scienceexhibitions.org)
  • At the beginning of Xenopus gastrulation, the presumptive anterior mesoderm cells located at the dorsal marginal zone (DMZ) roll inward at the midline of the blastopore lip in a process called involution. (nature.com)
  • Uninfected or Leishmania -infected thioglycollate-elicited peritoneal exudate cells were transferred from C57BL/6 to BALB/c mice or from Ly5.1 + to Ly5.1 - mice. (biomedcentral.com)
  • Here, we describe the detailed method to track Leishmania-infected myeloid cells migrating from the skin to lymphatic tissues by multiparametric flow cytometry. (bvsalud.org)
  • These methods involve labeling of infective Leishmania donovani parasites with fluorescent cell tracers and phenotyping of myeloid cells with fluorescent antibodies, to determine the infection status of migratory myeloid cells. (bvsalud.org)
  • We still know very little about the mechanisms controlling the ability of these cells containing live parasite or parasite fragments to remain in the infection site or to migrate to the draining lymph node. (biomedcentral.com)
  • This approach will allow the cells to migrate to dispersed tumor cells near the surgical site and also to track down tumor cells migrated to the normal brain parenchyma. (brainsciencefoundation.org)
  • We now report that DU145 human prostate cancer cells with reduced levels of PAK4 expression are unable to successfully migrate in response to HGF, have prominent actin stress fibres, and an increase in the size and number of focal adhesions. (silverchair.com)
  • During development and disease, cells migrate collectively in response to gradients in physical, chemical and electrical cues. (omicsdi.org)
  • More specifically, they show that the cells migrate into the CNS along vessels that pass directly between vertebral or calvarial bone marrow and the subarachnoid space. (medicilon.com)
  • showed that "exhausted" T cells are not inert but rather still active with a different set of skills notably with an active program allowing them to greater migrate into tumours. (preprintclub.com)
  • Although it is conceivable that cells can equally respond to gradients of soluble and/or immobilized chemokines, almost all cell biological information available about-gradient sensing is based on in vitro studies employing soluble gradients. (nature.com)
  • Previous studies have demonstrated that MSC treatment with pro-angiogenic proteins results in increased in vitro cell migration. (gatech.edu)
  • They found that α6 integrin-laminin interactions mediated the migration of ALL cells toward the cerebrospinal fluid in vitro . (medicilon.com)
  • In chapter 3, the ability of the developed pegylated and pH-sensitive liposomes containing DXR/C6-ceramide combination, to target, in vitro, cells of histological origin other than breast cancer (namely, ovarian cancer cells, including ovarian CSCs) was assessed. (uc.pt)
  • were used to establish a 3D organotypic in vitro model containing microtumor spheroids, macrophages, neutrophils, fibroblasts and endothelial cells, allowing for the analysis of tumor-stroma interactions in a controlled and modifiable environment. (ilexlife.com)
  • Results Senescent PA cells upregulate BCL-XL upon senescence induction and show dependency on BCL-XL for survival. (ibecbarcelona.eu)
  • Conclusions Our data demonstrate that BCL-XL is critical for survival of senescent PA tumor cells and provides proof-of-principle for the use of clinically available BCL-XL-dependent senolytics. (ibecbarcelona.eu)
  • The researchers' work focused on PI3K, a key regulator of signaling pathways needed for growth, survival, and invasion by cancer cells. (medicilon.com)
  • Tumor development and progression relies on a complex signaling network of deregulated pathways, as the PI3K/Akt pathway, sustaining cell proliferation, growth and survival. (uc.pt)
  • Using quantitative real-time microscopy techniques, including particle tracking microrheology and time-lapsed fluorescent microscopy, we monitored the effects of tumor-secreted soluble factors on intracellular rheology, cytoskeletal organization (including cytoskeletal filaments and focal adhesion proteins), and interaction with cell adhesion molecules. (gatech.edu)
  • Using microscopy, the researchers found that small blood vessels coated with laminin are sites with high levels of ALL cells, indicating that moving along these vessels may be how the cells reach the CNS. (medicilon.com)
  • Alternatively, the fluorescent ubiquitination-based cell-cycle signal FN-1501 (FUCCI) is really a widely used program to review the cell routine using video FUT3 microscopy [23,24,25,26]. (scienceexhibitions.org)
  • Structures such as nanofibers are engineered to promote tumor cell invasion and lead cancer cells to a site where the cells can be destroyed or captured for study. (freedomfromcancerchallenge.org)
  • For the current study, the research team looked at molecular differences between initial, or primary, triple-negative breast cancer sites and areas where it spread, or metastatic sites, among three different types of cells: mouse models, human cancers implanted into mice and samples of both primary and metastatic tissues taken from eight patients treated at The Johns Hopkins Hospital. (sciencedaily.com)
  • Key features â ¢ Cell-tracking of cell-trace-labeled parasites and monocytes from the skin to lymphatic tissues after transference into donor mice. (bvsalud.org)
  • However, parasite dissemination in malnourished mice was driven primarily by infected inflammatory monocytes, which showed increased CCR7 expression, greater intrinsic migratory capacity, and increased trafficking from skin to spleen. (bvsalud.org)
  • Upon showing that the ALL cells are unable to breach the BBB in mice, the researchers, led by Dorothy Sipkins, M.D., Ph.D., associate professor at Duke University Medical Center, looked at other approaches that could be taken by the cells to enter the CNS. (medicilon.com)
  • The treated animals had reduced signs of cancer invasion of the CNS when compared to mice that did not receive the molecule - the ALL cells remained in their bone marrow. (medicilon.com)
  • We recently showed that mice lacking B cells experience disseminated Chlamydia infection and develop ascites during Chlamydia primary infection. (uams.edu)
  • Stemness is the ability of stem cells to proliferate in an asymmetric way that allows them to maintain the reservoir of undifferentiated cells with stem cell identity, but also to produce new differentiated cells. (mdpi.com)
  • If you think these natural wonders should be protected for the multitude of endangered migratory and indigenous species like nesting pelicans, then please sign this petition. (antiguanice.com)
  • CellMAPtracer does apply for a number of two-dimensional (2D) cell migration assays, such as for example arbitrary migration and aimed migration. (scienceexhibitions.org)
  • Traditional multiwell (Boyden chamber) migration assays can have limitations, such as: (1) gradients are not linear, well-established, or controlled, (2) quantification of migratory cells is based on an endpoint measurement rather than a dynamic determination, and (3) imaging of the cells while they are migrating is currently not possible. (sigmaaldrich.com)
  • It is also very versatile in that the individual aspects of the invention ( e.g. nanofiber, chemical gradient and hydrogel composition) can be tailored to a specific purpose, e.g. accumulation in a specific area, collection of cancer cells for various assays or elimination of cancer cells. (freedomfromcancerchallenge.org)
  • Cells did not appear to follow blood vessels exclusively and histological analysis revealed that contact with radial glia was infrequent. (cosyne2023neurodev.com)
  • Cell migration is mainly governed by adhesion of cells to substrates (other cells or connective tissue) and by extracellular signalling molecules acting as motogenic stimuli or directional guidance cues 2 . (nature.com)
  • Two major concerns in cancer therapy are the short half-life, availability or excessive toxicity of the drugs, and the extreme invasiveness of the malignant tumor cells to infiltrate into the surrounding tissue and recur locally near the neurosurgical resection site. (brainsciencefoundation.org)
  • This is typically done by surgically removing the tumor or a portion of tissue containing cancer cells (a biopsy). (freedomfromcancerchallenge.org)
  • The bad news from our study is that cells from metastatic sites are super optimized for migration and resisting treatment," says Ewald. (sciencedaily.com)
  • The good news is that we identified several proteins called transcription factors that these cells require to handle the challenges of migrating and thriving at metastatic sites, and we may be able to design new therapies that target these transcription factors. (sciencedaily.com)
  • Collective cell migration plays an important role in wound healing, organogenesis, and the progression of metastatic disease. (omicsdi.org)
  • Migration and invasion require coordinated reorganisation of the actin cytoskeleton and regulation of cell-adhesion dynamics. (silverchair.com)
  • Underlying the development of neural crest is a gene regulatory network, described as a set of interacting signals, transcription factors, and downstream effector genes that confer cell characteristics such as multipotency and migratory capabilities. (wikipedia.org)
  • RQR8 is a 136-amino-acid epitope-based marker/suicide gene that enables clinical selection, cell tracking, and deletion in case of toxicity. (ashpublications.org)
  • A compact marker/suicide gene that utilizes established clinical-grade reagents and pharmaceuticals would be of considerable practical utility to T-cell cancer gene therapy. (ashpublications.org)
  • We have created a highly compact marker/suicide gene for T cells combining target epitopes from both CD34 and CD20 antigens (RQR8). (ashpublications.org)
  • We predict that RQR8 will make T-cell gene therapy both safer and cheaper. (ashpublications.org)
  • Dual RNA-Seq characterization of host and pathogen gene expression in liver cells infected with Crimean-Congo Hemorrhagic Fever Virus. (cdc.gov)
  • Despite its physiological significance and potential therapeutic applications, electrotactic collective cell movement is relatively less well understood. (omicsdi.org)
  • These results provide new insights in understanding the role of CAFs in promoting tumor cell invasiveness and may help to devise new targeted therapeutic approaches. (fupress.net)
  • The FUCCI program was originally created to indicate specific cell-cycle stages with a distinctive fluorescent signature within a spatiotemporal way using differentially shaded fluorescent tags on two cell-cycle-regulated proteins, that are Geminin and Cdt1 [27]. (scienceexhibitions.org)
  • Grant and co-workers enhanced the recognition of difference 1 (G1)/synthesis (S) and S/G2 transitions utilizing the proliferating cell nuclear antigen (PCNA)-interacting proteins (PIP)-FUCCI build [28]. (scienceexhibitions.org)
  • For some applications, cancer cells need to be dissociated from tumor into a single cell suspension using proteases (enzymes that cut proteins). (freedomfromcancerchallenge.org)
  • It is governed by cell-extrinsic molecular factors, which can either emerge in the soluble phase or as immobilized, often adhesive ligands. (nature.com)
  • An organizer population has been identified in the anterior end of the primitive streak of the mid-streak stage embryo, by the expression of Hnf3β , Gsc lacZ and Chrd , and the ability of these cells to induce a second neural axis in the host embryo. (silverchair.com)
  • The ability of CAFs to promote a 2-3 fold increase in SCD1 mRNA and protein expression as well as an induction of SREBP1 DNA binding activity has been shown in the two cancer cell lines. (fupress.net)
  • To clarify the possible role of tumor-stroma paracrine interaction in the previously reported improvement of cancer cell migratory ability, cocultures were set up in presence of neutralizing antibodies against hepatocyte growth factor, transforming growth factor-β or basic fibroblast growth factor. (fupress.net)
  • This suggested that the mode of action of the drug, the inhibition of PI3K, specifically decreased the ability of ALL cells to enter the CNS. (medicilon.com)
  • Beyond chemokines our approach is broadly applicable to alternative systems of chemo- and haptotaxis such as cells migrating along gradients of adhesion receptor ligands vs. any soluble cue. (nature.com)
  • The organization of cytoskeletal filaments and focal adhesion complexes changes rapidly during cell migration. (gatech.edu)
  • Moreover, these cells have a concomitant reduction in cell-adhesion turnover rates. (silverchair.com)
  • Decreased electrotaxis is also observed in isolated cells at intermediate electric fields, suggesting an adhesion-independent role of E-cadherin in regulating electrotaxis. (omicsdi.org)
  • In additional support of an adhesion-independent role of E-cadherin, isolated cells with reduced E-cadherin expression reoriented within an applied electric field 60% more quickly than control. (omicsdi.org)
  • Tracking the cell journey from the infection site in the skin to lymphoid tissues has been challenging, and is typically determined using fluorescent cell tracers, antibodies, or photoconvertible models. (bvsalud.org)
  • â ¢ Identification of migratory cells labeled with fluorescent cell tracers and antibodies by flow cytometry. (bvsalud.org)
  • Here, to study endogenous T cell population in humans, they developed a new technique using tumour biopsy slices that can recapitulate intravital imaging and developed non-stimulatory labelled antibodies to track human CD8+ T cells without impacting their biology. (preprintclub.com)
  • This preprint described a new imaging technique allowing the study of endogenous T cell migration in human tumours using developed non-stimulatory labelled antibodies that do not disturb the T cell biology. (preprintclub.com)
  • Loss of Fry function drastically affects the movement and morphological polarization of cells during gastrulation and disrupts dorsal mesoderm convergent extension, responsible for head-to-tail elongation. (nature.com)
  • Gastrulation movements are orchestrated by a small, heterogeneous group of cells with inductive and morphogenetic properties located in the dorsal lip of the blastopore (DBL) of the amphibian gastrula known as the Spemann-Mangold organizer or dorsal organizer. (nature.com)
  • We tracked hundreds of migrating cells in the dorsal forebrain of awake animals for up to 12 hours. (cosyne2023neurodev.com)
  • Immune cell trafficking in steady-state conditions and inflammatory cell recruitment into injured tissues is crucial for the surveillance of the immune system and the maintenance of body homeostasis. (bvsalud.org)
  • The finding that cancer cells take over a route used for neural migratory path finding leads the authors to suggest that "Future studies may reveal that this unique ALL trafficking pathway is involved in immune surveillance or inflammatory processes. (medicilon.com)
  • and (iii) the dramatic reductions to lymphocyte numbers and function 1-2 h after exercise reflects a transient and time-dependent redistribution of immune cells to peripheral tissues, resulting in a heightened state of immune surveillance and immune regulation, as opposed to immune suppression. (frontiersin.org)
  • Cell migration occurs in a variety of biological processes including wound healing, embryonic development, and immune response. (sigmaaldrich.com)
  • Then, using a non-biased classification of human tumours, they demonstrated that CD8+ T cells motility was correlated with their immune states. (preprintclub.com)
  • Hepatocyte growth factor (HGF) is associated with tumour progression and increases the invasiveness of prostate carcinoma cells. (silverchair.com)
  • Genetic engineering of T cells greatly increases the scope and potency of T-cell therapy: T-cell receptor (TCR) transfer allows targeting of intracellular cancer antigens, whereas chimeric antigen receptors (CARs) allow targeting of surface cancer or lineage-specific antigens. (ashpublications.org)
  • The researchers were tracking Kajtek's movements and posting his journey online before they lost signal on April 26. (iflscience.com)
  • How cancer cells enter the central nervous system has been an unanswered question for researchers and clinicians for decades. (medicilon.com)
  • The researchers used three different mouse models of ALL to study how human leukemia cells spread into the CNS. (medicilon.com)
  • The researchers found that the ALL cells were "grabbing onto the laminin surrounding these blood vessels and zipping down into the meninges region where cerebral spinal fluid circulates. (medicilon.com)
  • Researchers radio tracked migrating hawkmoths for up to 80 kilometers-the longest distance that any insect has been continuously monitored in the wild. (eurekalert.org)
  • When a tracking collar senses that its moose is dead, it sends a cell phone alert, allowing researchers to quickly find, collect and analyze the animal for parasites or other health problems. (nature.org)
  • She was born in Spain in 2015, at which point she was tagged with a GPS solar transmitter to allow researchers to track her life. (lu.se)
  • The current study, which followed radio-tagged individuals in a light aircraft, is the first to continuously study nocturnal migrating insects in the wild and represents the longest distance over which any insect has been continuously tracked in the field. (eurekalert.org)
  • It is specially designed for migratory beekeeping and continuously monitors hive weight. (broodminder.com)
  • We have long needed new treatment targets and options for triple negative breast cancers," says Andrew Ewald, Ph.D., the Virginia DeAcetis Professor in Basic Science Research and Director, Department of Cell Biology at the Johns Hopkins University School of Medicine and co-leader of the Cancer Invasion and Metastasis Program at the Johns Hopkins Kimmel Cancer Center. (sciencedaily.com)
  • Directed cell migration is critical across biological processes spanning healing to cancer invasion, yet no existing tools allow real-time interactive guidance over such migration. (omicsdi.org)
  • In the setting of allogeneic hematopoietic stem cell transplantation, donor lymphocyte infusions are frequently given to treat relapse of hematologic malignancies. (ashpublications.org)
  • Understanding the molecular mechanisms of neural crest formation is important for our knowledge of human disease because of its contributions to multiple cell lineages. (wikipedia.org)
  • Because of the importance of cell migration in angiogenesis, oncology, neurology, and especially immunology, there is an increased interest in understanding the underlying biological mechanisms at a single cell level. (sigmaaldrich.com)
  • However, their limited success on solid tumours suggests that first other mechanisms are also involved in this chronic T cell dysfunction but also that "exhausted" T cells might have important functions independently from those phenotypic markers. (preprintclub.com)
  • SCHEEPDOG: Programming Electric Cues to Dynamically Herd Large-Scale Cell Migration. (omicsdi.org)
  • Our stimulation scheme proves that cells effectively time-average electric field cues, helping to elucidate the transduction timescales in electrotaxis. (omicsdi.org)
  • Recently, navigation cues such as head direction compass cells have been identified in several insects. (jhu.edu)
  • CellMAPtracer works with with two- and three-color fluorescent ubiquitination-based cell-cycle signal (FUCCI) systems and enables an individual to accurately monitor several migration parameters through the entire cell cycle, having great potential to assist in new discoveries in cell biology thus. (scienceexhibitions.org)
  • Signalling networks that control the life or death of a cell are of central interest in modern biology. (sdbonline.org)
  • Marker genes enable detection and selection of T cells, whereas suicide genes enable selective destruction of T cells in case of toxicity. (ashpublications.org)
  • Marker genes enable measurement of transduction and allow selection of transduced cells, whereas suicide genes allow selective deletion of administered T cells in the face of toxicity. (ashpublications.org)
  • After epidermal injury, barrier repair requires activation of many wound response genes in epidermal cells surrounding wound sites. (sdbonline.org)
  • Exhausted T cells are mainly defined by a progressive but not complete loss of functions (cytotoxic, cytokine production, and homeostatic proliferation), changes in the epigenetic landscape, and the upregulation of inhibitory markers (such as PD1, LAG-3, and CTLA-4). (preprintclub.com)
  • Alternatively, cells could be tracked automatically by leveraging prior understanding of the motility and morphology of cells [5]. (scienceexhibitions.org)
  • Tumours with enriched phenotypic exhausted T cells displayed greater T cell motility in tumours. (preprintclub.com)
  • They used a speed cut-off for classifying T cells motility from tumours quite low. (preprintclub.com)
  • We showed previously that cruciferous vegetable constituent benzyl isothiocyanate (BITC) inhibits growth of cultured and xenografted human breast cancer cells and suppresses mammary cancer development in a transgenic mouse model. (omicsdi.org)
  • With optimized conditions, they assessed the migration behaviour of T cells in the different preserved tumour microenvironments from human tumour biopsies (collecting from various tumour types). (preprintclub.com)
  • A single mutation in Crimean-Congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells. (cdc.gov)
  • In vertebrates, bands of mobile cells fan out from the embryonic brain and spinal cord and then resettle in far-flung locales to form structures as diverse as bone or nerves. (stowers.org)
  • Thus, we investigated the influence of fibroblasts on the expression of Stearoyl- CoA desaturase 1 (SCD1), the main enzyme regulating membrane fluidity, as well as on the level and activity of its transcription factor, SREBP1, in breast cancer cells. (fupress.net)
  • Lastly, the underlying molecular mechanism of action of the nucleolin-mediated intracellular delivery of C6-ceramide to cancer cells of different histological origin was explored. (uc.pt)
  • Intracellular canaliculi form connections with adjacent cells, and these spaces subsequently fuse, forming a confluent lumen. (medscape.com)
  • That is specially the full case when tracking a dense population of cells with low contrast and inadequate gap size. (scienceexhibitions.org)
  • Tracking boils down to full life-cycle conservation," Mehlman says. (nature.org)
  • â ¢ Description of a double-staining technique with fluorescent cell tracers to determine cell and parasite dissemination from the skin to lymphoid tissues. (bvsalud.org)
  • The specific demonstration of this technology is its use to guide brain cancer (glioblastoma multiforme) cells to a location outside of the cortex where they can be safely killed by contact with a drug-containing hydrogel. (freedomfromcancerchallenge.org)