Microtubule intracellular cell cycle. Medical search. Frequent questions

Total cell lysates (15 µg total protein) from A431, SH-SY5Y, MCF-7 (reduced expression control), and NIH3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) Purified anti-EG5 Antibody, clone 10C7, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. (biolegend.com)
Microtubules (kinesin, dynein and intracellular transport, dynamics of microtubules and motor proteins). (pmf.hr)
Vincristine inhibits cellular mitosis by inhibition of intracellular tubulin function, binding to microtubules, and synthesis of spindle proteins in the S phase. (medscape.com)
The proteasome pathway is an enzyme complex existing in all cells, which degrades ubiquitinated proteins that control the cell cycle and cellular processes and maintains cellular homeostasis. (medscape.com)
Observations reported in this study suggest that these BER pathway components are microtubule-associated proteins (MAPs) themselves or utilize yet to be identified MAPs to bind microtubules in order to regulate their intracellular trafficking and activities during the cell cycle. (neb.com)
Our contribution to the third edition of the Encyclopedia of Biological Chemistry is a further reading section on microtubule plus and minus end binding proteins. (mechanochemistry.org)
Phosphatidylinositol 4,5-bisphosphate (PI4,5P2, hereafter referred to as PIP2) either binds to intracellular proteins and directly modulates their subcellular localization and activity or acts as a precursor for the generation of other second messengers. (biomedcentral.com)
Ingenuity pathway analysis (IPA) identified 256 signaling pathways and 184 cellular functional proteins that were regulated by DMXAA in A549 cells. (elsevierpure.com)
What started as an informal meeting among a handful of labs involved in intracellular trafficking has grown to include tens of labs, both young and established, all interested in understanding the cytoskeleton and motor proteins in the context of intracellular trafficking. (silverchair.com)
High levels of intracellular calcium ion activate proteolytic enzymes (known as calpains) that break down many cell proteins, particularly those in the cytoskeleton of neurons (spectrin, neurofilament and microtubule-associated protein). (benbest.com)
Many of the components of axoplasm, such as soluble proteins and cytoskeleton, are slowly transported, taking weeks to months to travel the length of axons longer than a few millimeters after being synthesized in the cell body. (mblwhoilibrary.org)
In addition, phosphorylation of proteins implicated with early cell contractility and cell migration, such as microtubule-associated protein 1B and paxillin, respectively, was not detected in the VACV-infected KO cells. (ista.ac.at)
specifically acted, the FGFR preventing download manual of structural kinesiology 2011 traffics found by intracellular intracellular matrix proteins. (erik-mill.de)
Molecules can be transported around a cell by so-called motor proteins that move along a network of filaments called microtubules. (elifesciences.org)
Many motor proteins-including the kinesin family of these proteins-can only move in one direction along a microtubule. (elifesciences.org)

Nerve cells: signal transduction (action potential), neurotransmitters and receptors. (pmf.hr)
16. To explain molecular mechanisms involved in signal transduction in nerve cells. (pmf.hr)
A ubiquitous phosphoprotein that serves as an intracellular substrate for a variety of SIGNAL TRANSDUCTION PATHWAYS. (bvsalud.org)
Commonly used drugs act to block signal transduction and cell cycle progression in different phases, thereby blocking smooth muscle cell (SMC) proliferation or intimal hyperplasia in the stented arterial site. (statpearls.com)
MTs serve as an essential component of a cell's cytoskeleton as they regulate and participate in a variety of cellular functions that include motility, morphology, intracellular transport, signal transduction, and cell division (Fig. 1). (cytoskeleton.com)
Recent evidence indicates that Taxol alters specific intracellular signal transduction events, such as the activation of Raf-1 kinase, that may be essential for drug-induced apoptosis. (elsevierpure.com)
Area of expertise: Intracellular signal transduction controlling plant growth and development, and agricultural improvement. (jipb.net)

Triclabendazole(CGA89317) is a benzimidazole, it binds to tubulin impairing intracellular transport mechanisms and interferes with protein synthesis. (chemspider.com)
Target: Microtubule/Tubulin;Triclabendazole treatment produces percentage decreases of the fluke egg output by 15.3%, 4.3% and 36.6%, respectively, in sheep, dairy cows and heifers, these results indicate the presence of TCBZ-resistant Fasciola hepatica in sheep and cattle on this farm [1]. (chemspider.com)
Microtubules are dynamic, non-covalent polymers consisting of α- and ß-tubulin subunits that are involved in a wide range of intracellular processes. (bvsalud.org)
The polymerization and dynamics of microtubules are regulated by many factors, including small molecules that interact with different sites on the tubulin dimer. (bvsalud.org)
Colchicine binding site inhibitors (CBSIs) destabilize microtubules and inhibit tubulin polymerization, leading to cell cycle arrest. (bvsalud.org)
Microtubules (MTs) are comprised of α/β tubulin heterodimers which have polymerized into cylinderical structures. (cytoskeleton.com)
Franck Perez mainly focuses on Cell biology, Microtubule, Tubulin, Microtubule nucleation and Astral microtubules. (research.com)
His Tubulin research is multidisciplinary, relying on both Cell culture and In vivo. (research.com)

Regulation of the cell cycle: mitosis, meiosis. (pmf.hr)
PHOSPHORYLATION of stathmin occurs during CELL CYCLE progression, and stathmin functions as a microtubule-destabilizing protein that promotes MICROTUBULE depolymerization during INTERPHASE and late MITOSIS. (bvsalud.org)
Another drug category is taxanes, which interfere with microtubule function, which is necessary for the M phase (mitosis). (statpearls.com)
Kinesin-II is not essential for mitosis and cell growth in Chlamydomonas. (bio.net)
8-oxoguanine DNA glycosylase (OGG1), a major DNA repair enzyme in mammalian cells and a component of the base excision repair (BER) pathway, was recently shown to be associated with the microtubule network and the centriole at interphase and the spindle assembly at mitosis. (neb.com)
Depletion of Raf-1 resulted in the accumulation of cells in the G 2 -M phase of the cell cycle, suggesting that Raf-1 may play an important role in the passage through mitosis. (elsevierpure.com)
9 nM), cell death may occur after an aberrant mitosis by a Raf-1 independent pathway, whereas at higher Taxol concentrations (≤9 nM) cell death may be the result of a terminal mitotic arrest occurring by a Raf-1-dependent pathway. (elsevierpure.com)
His research in Golgi apparatus intersects with topics in Endocytic cycle and Mitosis. (research.com)
The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. (cancerindex.org)
This protein is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle. (antibodypedia.com)

CEV induces the formation of actin tails that drive CEV particles away from the cell and is important for cell-to-cell spread. (microbiologyresearch.org)
In the first section, we will focus on the cytoskeleton and summarize our knowledge on how and why the infection alters F-actin, intermediate filaments and microtubules. (caister.com)
Although the formation of intracellular mature virus (IMV) was not affected in MKK4/7- or JNK1/2-knockout (KO) cells, we did note an accentuated deregulation of microtubule and actin network organization in infected JNK1/2-KO cells. (ista.ac.at)
Myosins, which are actin-based motor molecules with ATPase activity, include unconventional myosins that serve in intracellular movements. (nih.gov)
Together with CLIP-190 (CAP-Gly domain-containing/cytoplasmic linker protein 190), Jar may coordinate the interaction between the actin and microtubule cytoskeleton. (nih.gov)

To determine whether Raf-1 kinase activation occurs at different concentrations of Taxol and in response to disruption of the normal microtubule cytoskeleton, A549 cells were treated with different concentrations of Taxol after which Raf-1 activation and the microtubule cytoskeleton were analyzed. (elsevierpure.com)
However, disruption of the normal microtubule cytoskeleton was seen at lower Taxol concentrations (1-7 nM), indicating that this process begins in the absence of Raf-1 activation. (elsevierpure.com)
His study focuses on the intersection of Microtubule and fields such as Microtubule organizing center with connections in the field of Detyrosination and Cytoskeleton. (research.com)

Its mechanism of action is complex and includes depolymerization of microtubules. (medscape.com)
The cell cycle consists of the sequential G1, S, G2, and M phases with MT polymerization and depolymerization (i.e. (cytoskeleton.com)
The spindle microtubule (MT) exhibits highly regulated dynamic instability, with frequent polymerization and depolymerization occurring at both the plus and minus ends. (biomedcentral.com)

Vaccinia virus produces four different types of virion from each infected cell called intracellular mature virus (IMV), intracellular enveloped virus (IEV), cell-associated enveloped virus (CEV) and extracellular enveloped virus (EEV). (microbiologyresearch.org)
An antigenic difference between intracellular and extracellular rabbitpox virus. (microbiologyresearch.org)
Extracellular vaccinia virus formation and cell-to-cell virus transmission are prevented by deletion of the gene encoding the 37,000-Dalton outer envelope protein. (microbiologyresearch.org)
Recent experimental findings revealed considerable complexity to the underlying mechanosensory mechanisms that transmit extracellular stimuli to intracellular signaling many of which include primary cilia. (aimspress.com)
A sodium/potassium (Na + /K + ) pump keeps extracellular potassium low and extracellular sodium high compared to intracellular concentrations. (benbest.com)
Exocytosis and related Cell and Extracellular. (research.com)

Cell death and stem cells: apoptosis, necrosis, embryonic stem cells, bone marrow stem cells. (pmf.hr)
14. To describe mayor signs of controlled cell death (apoptosis). (pmf.hr)
As a microtubule (MT) cross-linking enzyme, KSP plays a critical role in mitotic spindle pole separation, and its inhibition results in the formation of monoaster spindles which is thought to lead to mitotic catastrophe and apoptosis (Fig. 1). (cytoskeleton.com)
Subsequently, the effects of DMXAA on cell cycle distribution, apoptosis, autophagy, and reactive oxygen species (ROS) generation were experimentally verified. (elsevierpure.com)
Western blotting assays demonstrated that DMXAA induced apoptosis via a mitochondria-dependent pathway and promoted autophagy, as indicated by the increased level of cytosolic cytochrome c, activation of caspase 3, and enhanced expression of beclin 1 and microtubule-associated protein 1A/1B-light chain 3 (LC3-II) in A549 cells. (elsevierpure.com)
Calcitriol elicits anti-tumor effects mainly through the induction of cancer cell apoptosis, cell cycle arrest, differentiation, angiogenesis and the inhibition of cell invasiveness by a number of mechanisms. (jcancer.org)
The broad spectrum anti-tumor effects of calcitriol and analogs are mostly based on inhibition of cancer cell proliferation and invasiveness, induction of differentiation and apoptosis, and promotion of angiogenesis. (jcancer.org)
Indeed apoptosis is inhibited in senescent cells. (globale-dermatologie.com)
Role of PDL-1 in NSCLC chemoresistance was assessed using CRISPR/Cas9 knockdown and various functional assays including MTT, cell invasion, sphere formation, and cell apoptosis. (cdc.gov)
Mechanistically, an upregulation of PDL-1 in chemotherapy-treated CAFs led to an increase in hepatocyte growth factor (HGF) secretion, which stimulates cancer progression, cell invasion, and stemness of lung cancer cells, while inhibiting apoptosis. (cdc.gov)

Eg5 is localized at the centrosomes, spindle microtubules, and intracellular bridge. (biolegend.com)

Our results offer new insight into the mechanisms of microtubule polymerization inhibition by colchicine and its analogs. (bvsalud.org)
My lab focuses on the mechanisms that generate specific microtubule arrays in polarised cells, the dynamic interactions of microtubule tips with intracellular structures and the cell cortex to control cell shape changes and the transport along microtubule arrays mediated by dynein and kinesins. (mechanochemistry.org)
This study aimed to examine the proteomic response to DMXAA and unveil the global molecular targets and possible mechanisms for the anticancer effect of DMXAA in NSCLC A549 cells using a stable-isotope labeling by amino acids in cell culture (SILAC) approach. (elsevierpure.com)
Although the ability of Taxol to stabilize cellular microtubules is well accepted, the mechanisms by which Taxol induces growth arrest and cell death remain unclear. (elsevierpure.com)
These studies suggest that Taxol-mediated cell death may result from two different mechanisms. (elsevierpure.com)
Three decades of multidrug resistance research have identified a number of mechanisms by means of which cancer cells elude the effects of chemotherapeutic agents. (biomedcentral.com)
5-FU inhibits tumor cell growth through at least 3 different mechanisms that ultimately disrupt DNA synthesis or cellular viability. (medscape.com)
Mechanisms regulating cardiomyocyte cell cycle arrest are of great interest partly because reversing this process could provide a way to stimulate cardiac regeneration after injury [ 17 ]. (springer.com)
In this study, we investigated programmed death-ligand 1 (PDL-1) as a potential biomarker for CAF-induced chemoresistance and evaluated its role and the underlying mechanisms of chemoresistance in non-small cell lung cancer (NSCLC). (cdc.gov)

This is the 6th step in the glycolytic breakdown of glucose, an important pathway of energy and carbon molecule supply which takes place in the cytosol of eukaryotic cells. (wikipedia.org)

Depletion of PIP4KIIγ enhanced the spindle pole accumulation of mitotic centromere-associated kinesin (MCAK), a microtubule (MT)-depolymerizing kinesin, and resulted in a less stable spindle pole-associated MT. Depletion of MCAK can ameliorate PIP4KIIγ depletion-induced spindle abnormalities. (biomedcentral.com)
Live cell imaging and immunolabeling for microtubules and the centralspindlin constituent and kinesin-related protein, MKLP1, demonstrated that furrowing in ammonia-activated eggs was associated with aligned arrays of centralspindlin-linked, opposed bundles of antiparallel microtubules. (mblwhoilibrary.org)
Previous studies have indicated that kinesin-14 motors are non-processive enzymes, working in the context of multi-motor ensembles that collectively organize microtubule networks. (elifesciences.org)

Microtubules are essential for chromosome segregation, intracellular transport, positioning of organelles, directed cell migration and differentiation. (mechanochemistry.org)
Using time-lapse light microscopy of in vitro haptomonad-like cells, we identified five stages of haptomonad-like cell differentiation, and showed that calcium is necessary for Leishmania adhesion to the surface in vitro. (brookes.ac.uk)

Effects of chloroplast DNA content on the cell proliferation and aging in Chlamydomonas reinhardtii. (bio.net)
These targeted molecules and signaling pathways were mostly involved in cell proliferation and survival, redox homeostasis, sugar, amino acid and nucleic acid metabolism, cell migration, and invasion and programed cell death. (elsevierpure.com)
These specially tuned fields have no effect on quiescent cells while having an anti-proliferation and destructive effect on mitotic cells. (biomedcentral.com)
Bowen, 2004} detected keratinocyte proliferation by PCNA (proliferating cell nuclear antigen) marking, 13,9% of keratinocytes were stained, compared with 6,4% in normal skin. (globale-dermatologie.com)
OPC architecture, proliferation and oligodendro-/myelino-genesis are finely regulated by the interplay of cell-intrinsic and extrinsic factors. (nature.com)
Our results unveil a novel player in the complex regulation of OPC biology, on the one hand showing that JNK1 ablation cell-autonomously determines alterations of OPC proliferation and branching architecture and, on the other hand, suggesting that JNK1 signaling in OLs participates in myelination in vivo. (nature.com)
Both a cell-intrinsic program and environmental factors participate in the regulation of OPC architecture, proliferation and oligodendro-/myelino-genesis. (nature.com)
Antineoplastic agents inhibit cell growth and proliferation. (medscape.com)
Platinum compounds inhibit cell growth and proliferation. (medscape.com)
However, potentially erroneous characterization of cytotoxicity and cell proliferation can arise if verification of baseline interaction of test article with method employed is neglected. (cdc.gov)
Non-tumorigenic Beas-2B cells were treated with graded concentrations of benzo[a]pyrene (B[a]P) for 24 and 48h prior to cytotoxicity and proliferation assessment with commonly used MTT, MTS, WST1, and Alamar Blue assays. (cdc.gov)

Cytoskeletal dynamics and spindle assembly during cell division. (irb.hr)
Using atomistic molecular dynamics simulations and binding free energy calculations, we show that CBSIs act as protein-protein interaction inhibitors and destabilize interlinkage between α and ß subunits, which is crucial for longitudinal contacts in the microtubule lattice. (bvsalud.org)
When expressed as a fusion protein with eYFP in cultured cells, Sec31B-F associates with the endoplasmic reticulum and with vesicular-tubular clusters, displays restricted intracellular movement characteristic of COPII vesicle dynamics, co-distributes on organelles with Sec13, Sec31A and Sec23 (markers of the COPII coat), and concentrates with ts045-VSV-G-CFP (VSV-G) when examined early in the secretory pathway or after temperature or nocodazole inhibition. (biologists.com)
All these processes require the organisation of microtubules into arrays with different geometry and density and the proper regulation of dynamics and interactions at the microtubule ends. (mechanochemistry.org)
MT dynamics) playing a key role in the normal progression of this cycle to insure proper cell division (Fig. 1). (cytoskeleton.com)
The disruption of MT dynamics, and thereby the cell cycle, leads to cell death. (cytoskeleton.com)

GAPDH from Candida albicans is found to cell-wall associated and binds to Fibronectin and Laminin. (wikipedia.org)
In genomic pathways, calcitriol binds to intracellular vitamin D receptor (VDR), which subsequently heterodimerizes with another nuclear receptor retinoid X receptor (RXR). (jcancer.org)
found that the Kar3-Cik1 motor binds to and transports a protein complex that is crucial for separating chromosomes during cell division. (elifesciences.org)

Bacteria of the Chlamydia genus are obligate intracellular pathogens that reside at all times within a membrane-bound cytoplasmic compartment called the chlamydial inclusion. (caister.com)
All AM fungi are obligate biotrophs, i.e., they rely on living host cells to complete their life cycle ( Smith and Read, 2008 ). (frontiersin.org)
Related to fungi, they are obligate intracellular organisms that spread among hosts through a spore stage ( 1 ). (cdc.gov)

Activation of cytoplasmic dynein through microtubule crossbridging. (mechanochemistry.org)

Indeed, in a clone of cells, only one of the alleles is expressed, because the same X chromosome is inactivated by lyonisation{Allen, 1992}, in a clone, whereas in normal {Nakamura, 2001} skin (polyclonal), the inactivated HUMARA gene allele isn't always on the same X chromosome. (globale-dermatologie.com)
No chromosome was found contrary to an extra chromosome 5 in the basal cell carcinoma. (globale-dermatologie.com)

This intracellular complex increases the expression of p27 and blocks the progression of the cell cycle from the G1 phase to the S phase (DNA synthesis). (statpearls.com)
The FA2 gene of Chlamydomonas encodes a NIMA family kinase with roles in cell cycle progression and microtubule severing during deflagellation. (bio.net)

Livers treated with NMP combined with BMMSCs showed better liver function, relieved histopathological damage, reduced oxidative stress injury and ferroptosis, and the mechanism of reduction was associated with downregulation of intracellular reactive oxygen species (ROS) and free Fe²⁺ levels. (medscimonit.com)

9. To describe molecules and processes involved in the movement and orientation of the cells. (pmf.hr)
11. To list molecules involved in communication and adhesion of metazoan,s cells. (pmf.hr)
While single molecules of dynein are predominantly static or diffusive on single microtubules, they walks processively on the microtubule bundles they form. (mechanochemistry.org)
The proteomic data showed that treatment with DMXAA modulated the expression of 588 protein molecules in A549 cells, with 281 protein molecules being up regulated and 306 protein molecules being downregulated. (elsevierpure.com)
TTFields can induce a number of biological responses, including mitotic catastrophe, to kill cancer cells by targeting polarizable intracellular molecules. (conditionmed.org)
In most cells, kinesins tend to transport other molecules away from the center and towards the cell edge. (elifesciences.org)
studied how a molecular motor made up of Kar3 and Cik1 moves along microtubules using sensitive microscopy that allows single molecules to be observed. (elifesciences.org)

Protein phosphorylation regulates actomyosin-driven vesicle movement in cell extracts isolated from the green algae, Chara corallina. (bio.net)
The vesicle-associated mob SLC6A3 attracts the hands-on transamidase thermogenesis, DAT which inhibits the muscle of cell from the rare nucleotide( Vandenbergh DJ et al, 2000). (erik-mill.de)

We will also discuss the interaction of the inclusion with the centrosome, including its potentially negative consequences for the host cell. (caister.com)
Mathematical modeling of the primary cilium as a fluid-structure interaction problem highlights the importance of basal anchorage and the anisotropic moduli of the microtubules. (aimspress.com)
Interaction with microtubules, located at the mitotic spindle for the mitotic role. (univ-amu.fr)

The role of microtubule pivoting in formation of complex structures such as microtubule bundles and mitotic spindles. (irb.hr)
Dynein polarity-sorts microtubule bundles by selectively sliding anti-parallel microtubules apart. (mechanochemistry.org)
Their force output doubles on microtubule bundles and we propose a model whereby the separation of the motor domains during crossbridging activates the motor. (mechanochemistry.org)

Regulation of the expressions of HCO3- uptake and intracellular carbonic anhydrase in response to CO2 concentration in the marine diatom Phaeodactylum sp. (bio.net)
On the other hand, non-genomic actions may indirectly affect gene transcription via the regulation of intracellular signaling pathways that target transcription factors ( 3 ). (jcancer.org)

Calcitriol induces a number of non-genomic responses including rapid intestinal absorption of calcium, release of calcium from intracellular stores, opening of voltage-gated calcium and chloride channels, and the activation of protein kinase C, protein kinase A, phosphatidylinositol-3 kinase (PI3K) and phospholipase C ( 4 ). (jcancer.org)

We show that human dynein is activated when it crossbridges two microtubules. (mechanochemistry.org)

We quantified the number and location of rhoptries and micronemes within cells and show a highly organised gateway for trafficking and docking of rhoptries, micronemes and microtubule-associated vesicles within the conoid around a set of intra-conoidal microtubules. (brookes.ac.uk)

In this study, we determined whether other participants in the BER pathway also bind microtubules in situ and in vitro. (neb.com)
Results from in situ localization, microtubule co-precipitation and site-directed photochemical experiments showed that recombinant human DNA Pol beta and recombinant mNEIL2 associated with microtubules in situ and in vitro in a manner similar to that shown earlier for another BER pathway component, OGG1. (neb.com)
further, the stimulation of this pathway requires postpenetration, prereplicative events in the viral replication cycle. (ista.ac.at)
Franck Perez focuses on Cell biology, Golgi apparatus, Endoplasmic reticulum, Secretory pathway and Microtubule. (research.com)
His primary scientific interests are in Cell biology, Golgi apparatus, Secretory pathway, Endoplasmic reticulum and Cancer research. (research.com)
His primary areas of investigation include Cell biology, Golgi apparatus, Endoplasmic reticulum, Secretory pathway and Microtubule. (research.com)

More recently (in 2019), I 'climbed uphill' to Oxford Brookes, where I worked as a Module Leader in Cell Biology before becoming the Bioimaging Manager. (brookes.ac.uk)

They also interfere with the polymerization processes of the microtubule spindle during cell division. (biomedcentral.com)

these 2 events, in turn, can inhibit growth of neoplastic cells. (medscape.com)

human breast cancer cells MCF-7 and their mitoxantrone-resistant sub lines MCF-7/Mx and human breast cancer cells MDA-MB-231 and their doxorubicin resistant MDA-MB-231/Dox cells. (biomedcentral.com)
TTFields had no effect on intracellular doxorubicin accumulation in both wild type and MDR cells. (biomedcentral.com)
The results indicate that TTFields alone and in combination with paclitaxel and doxorubicin effectively reduce the viability of both wild type and MDR cell sub-lines and thus can potentially be used as an effective treatment of drug resistant tumors. (biomedcentral.com)

Endosomal transport of ErbB-2: mechanism for nuclear entry of the cell surface receptor. (univ-amu.fr)
In the field of Cell biology, his study on Golgi apparatus and Transport protein overlaps with subjects such as Palmitoylation. (research.com)
His research related to Transport protein and Endosome might be considered part of Cell biology. (research.com)
The role of microtubules in secretory protein transport. (research.com)

Intracellular establishment of AM fungi requires the VAPYRIN protein which is induced in colonized cells, and which localizes to numerous small mobile structures of unknown identity (Vapyrin-bodies). (frontiersin.org)

In this chapter, we will discuss how these bacteria orchestrate the intricate relationship of the inclusion with most of the cellular elements of their host cell. (caister.com)
Of all cellular specializations, the axon is especially distinctive because it is a narrow cylinder of specialized cytoplasm called axoplasm with a length that may be orders of magnitude greater than the diameter of the cell body from which it originates. (mblwhoilibrary.org)

His Cell biology research incorporates elements of HEK 293 cells, Endocytic cycle and ATG5. (research.com)

Flow cytometric analysis showed that DMXAA significantly induced G 1 phase arrest in A549 cells. (elsevierpure.com)
Moreover, DMXAA significantly promoted intracellular ROS generation in A549 cells. (elsevierpure.com)

His studies deal with areas such as Astral microtubules and Microtubule nucleation as well as Microtubule. (research.com)

and microtubule-disturbing agents paclitaxel and docetaxel. (jcancer.org)

Area of expertise: plant molecular cell biology and intercellular signaling in plant reproduction. (jipb.net)

Furthermore, VACV infection induced alterations in the cell contractility and morphology, and cell migration was reduced in the JNK-KO cells. (ista.ac.at)

elicits antiproliferative and proapoptotic activities in vitro in solid and hematologic tumor cells. (medscape.com)
Reference: The murine DNA glycosylase NEIL2 (mNEIL2) and human DNA polymerase beta bind microtubules in situ and in vitro. (neb.com)
To do this we combine quantitative live cell imaging approaches with in vitro reconstitution assays. (mechanochemistry.org)
Hydrogen peroxide was used to induce an oxidative stress model of hepatocyte IAR-20 cells to evaluate the protective effects of BMMSCs in vitro. (medscimonit.com)

Rapamycin agents bind to the intracellular protein, FKBP-12, that inhibits the protein kinase mammalian target of rapamycin (mTOR). (statpearls.com)

Here we show that vascular edhesion protein-1 (VAP-1) is required for proper cell migration, adhesion, contractility and clonogenic behaviour. (mechanochemistry.org)

Two schools of thought exist, one states that SK develops from an accumulation of senescent cells, whereas the other states that cells hyperproliferate. (globale-dermatologie.com)

2020. Cell Death Dis. (biolegend.com)
J Cell Sci (2020) 133 (8): jcs245928. (silverchair.com)

Dissociation of progeny vaccinia virus from the cell membrane is regulated by a viral envelope glycoprotein: effect of a point mutation in the lectin homology domain of the A34R gene. (microbiologyresearch.org)
For reciprocal nutrient exchange, AM fungi form intracellular arbuscules that are surrounded by the peri-arbuscular membrane. (frontiersin.org)
Most of the metabolic energy of neurons is expended on maintaining ion gradients across the cell membrane. (benbest.com)
Voltage-gated ion channels and ion-exchangers in the cell membrane also regulate ion concentrations. (benbest.com)
The various areas that he examines in his Cell biology study include Secretion and Cell membrane. (research.com)
The study incorporates disciplines such as Cell growth, Glycosylation, Cell membrane, Enzyme and Glycosphingolipid in addition to Golgi apparatus. (research.com)
His Cell membrane research integrates issues from Endocytosis, Secretion assay and Focal adhesion. (research.com)
His Microtubule study integrates concerns from other disciplines, such as Focal adhesion, Exocytosis, Secretion assay, Secretion and Cell membrane. (research.com)

Reversible proteasome inhibition disrupts pathways supporting cell growth, thus decreasing cancer cell survival. (medscape.com)
Notably, exposure of cancer cells to TTFields has been shown to result in immunogenic cell death, which is reflected by damage-associated molecular patterns and the formation of micronuclei that further activate DNA sensor-related inflammatory pathways, indicating the immunomodulatory role for TTFields. (conditionmed.org)

Each subunit has a region called a motor domain (also known as its 'head') that can bind to the microtubule and to a molecule called ATP, which provides the energy required for the motor to step forward. (elifesciences.org)
The head of the non-motor protein cannot bind to ATP, although it can bind to microtubules. (elifesciences.org)

Exposure of cancer cells to chemotherapeutic agents may result in reduced sensitivity to structurally unrelated agents, a phenomenon known as multidrug resistance, MDR. (biomedcentral.com)

Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetoc hore fibers of the mitotic spindle by acting as inter-microtubule bridge (PubMed:15858577, PubMed:16968737, PubMed:21297582). (univ-amu.fr)
Myosin-VI appears to be involved in a very early step of clathrin-mediated endocytosis in polarized epithelial cells. (nih.gov)

The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. (cancerindex.org)

For these studies we use neurons that are maintained in cell culture as well as genetically-modified strains of mice that are at increased risk for developing neurodegenerative conditions analogous to those observed in humans. (uml.edu)

The protein encoded by this gene plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. (antibodypedia.com)

Excessive glutamate release resulting in excessive Ca +2 entry into cells is the excitotoxicity which initiates the brain ischemic damage seen in stroke and cardiac arrest. (benbest.com)
Interest in ALMS1 is heightened by the recent discovery of its involvement in neonatal cardiomyocyte cell cycle arrest, a process with potential relevance to regenerative medicine. (springer.com)

The production of several different virus particles in the VV replication cycle represents a coordinated strategy to exploit cell biology to promote virus spread and to aid virus evasion of antibody and complement. (microbiologyresearch.org)
Multidrug resistance (MDR) [ 1 ] is encountered when cancer cells are exposed to chemotherapeutic agents for a few replication cycles. (biomedcentral.com)
Cell cycle-specific with activity in the S-phase as single agent and has for many years been combined with biochemical modulator leucovorin.Has activity as single agent that inhibits DNA replication and transcription. (medscape.com)

The purpose of this study is to investigate cell growth inhibition of wild type and the corresponding MDR cells by Tumor Treating Fields - TTFields, a new cancer treatment modality that is free of systemic toxicity. (biomedcentral.com)
TTFields caused a similar reduction in the number of viable cells of wild type and MDR cells. (biomedcentral.com)
Treatments by TTFields/drug combinations resulted in a similar increased reduction in cell survival of wild type and MDR cells. (biomedcentral.com)

Multicellularity: cell-cell interactions, interactions of cells with cell matrix, embryogenesis, formation of organs and tissue organization. (pmf.hr)
Patterns are ubiquitous in living systems and underlie the dynamic organization of cells, tissues, and embryos. (mpi-cbg.de)
Franck Perez works mostly in the field of Microtubule nucleation, limiting it down to concerns involving Nocodazole and, occasionally, Golgi organization, Organelle and Subcellular localization. (research.com)

this raises questions about how the Kar3 motor protein moves along the microtubule, and whether this affects the roles the motor performs. (elifesciences.org)

The apical complex of apicomplexan parasites is essential for host cell invasion and intracellular survival and as the site of regulated exocytosis from specialised secretory organelles called rhoptries and micronemes. (brookes.ac.uk)

the primary cilium, in Methods in Cell Biology, Vol. 94: Primary Cilia (ed. (aimspress.com)
My background is in Cell Biology , and I spent most of my scientific career doing light and electron microscopy, biochemistry, and molecular biology of cancer cells and protozoan parasites (see references). (brookes.ac.uk)

Primary cilia are non-motile, solitary (one per cell) microtubule-based organelles that emerge from the mother centriole after cells have exited the mitotic cycle. (aimspress.com)

It is an alkylating agent, and its mechanism of action of active metabolites may involve cross-linking of DNA, which may interfere with growth of normal and neoplastic cells. (medscape.com)

Arbuscular mycorrhiza (AM) is a symbiosis between plants and AM fungi that requires the intracellular accommodation of the fungal partner in the host. (frontiersin.org)
Reorganization of host cells during intracellular accommodation of AM fungi has been described in considerable detail. (frontiersin.org)

IEV is formed by wrapping of IMV with intracellular membranes, and is an intermediate between IMV and CEV/EEV that enables efficient virus dissemination to the cell surface on microtubules. (microbiologyresearch.org)
Potassium ions rush out of the cell while sodium & chloride ions rush inward as the cell membranes depolarize. (benbest.com)
Postsynaptic membranes contain two voltage-gated calcium channels (L-type & T-type) as well as a sodium/calcium exchanger, but the NMDA channel is particularly adept at allowing large amounts of calcium ion to enter the cell. (benbest.com)

Nat Rev Mol Cell Biol , Art. (mpi-cbg.de)
Cell Biol. (aimspress.com)

Both mutations reduce microtubule binding, but are fast and processive motors in single molecule assays. (mechanochemistry.org)

VEGF uncouples its truncated cell through phase with thyroid muscle transcription factors VEGFR, enough used on inner Lo-like diseases. (erik-mill.de)

The establishment of AM requires fundamental reorganization of the host cells to allow intracellular accommodation of the fungal symbiont. (frontiersin.org)

Anatomy36

Organisms15

Diseases1

Chemicals and Drugs107

Analytical, Diagnostic and Therapeutic Techniques and Equipment23

Phenomena and Processes75

Disciplines and Occupations1

Technology, Industry, Agriculture3

Information Science4

Health Care1

Proteins15

Signal transduction7

Tubulin8

Mitosis10

Actin5

Cytoskeleton3

Depolymerization3

Extracellular6

Apoptosis10

Spindle microtubules1

Mechanisms9

Cytosol1

Kinesin3

Differentiation2

Proliferation11

Dynamics6

Binds3

Obligate3

Cytoplasmic1

Chromosome2

Progression2

Reactive oxygen1

Molecules7

Vesicle2

Interaction3

Bundles3

Regulation2

Induces1

Dynein1

Vesicles1

Pathway6

20191

Processes1

Inhibit1

Doxorubicin3

Transport4

Structures1

Cellular2

Endocytic1

A5492

Astral1

Paclitaxel1

Molecular Cell Bi1

Morphology1

Vitro4

Kinase1

Migration1

Accumulation1

20202

Membrane8

Pathways2

Bind2

Exposure of cancer cells1

Clathrin2

Phosphorylation1

Neurons1

Centrosome1

Arrest2

Replication3

TTFields3

Organization3

Motor protein1

Survival1

Biology2

Centriole1

Interfere1

Fungi2

Membranes3

Biol2

Assays1

Transcription1

Symbiont1