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  • nmol
  • Estimates of apparent K m and V max for propofol hydroxylation by microsomes from a male beagle dog were 7.3 μM and 3.8 nmol/mg/min, respectively. (aspetjournals.org)
  • At a substrate concentration of 20 μM, propofol hydroxylase activity was significantly lower ( p = .032) in greyhound microsomes (1.7 ± 0.4 nmol/mg/min) compared with beagle microsomes (5.1 ± 1.3 nmol/mg/min) but was not statistically different ( p = .42) compared with mixed-breed microsomes (3.1 ± 1.2 nmol/mg/min). (aspetjournals.org)
  • Tissues
  • The catalytic efficiencies of microsomes from known susceptible tissues/subcompartments are 10 and 250 fold higher than in rat airway and monkey alveolar subcompartments, respectively. (osti.gov)
  • insect
  • To get microsomes containing a specific CYP or for high amounts of active enzyme, microsomes are prepared from Sf9 insect cells or in yeast via heterologous expression. (wikipedia.org)
  • The insect cell microsomes are clumpy when isolated either in solutions containing high salt (0.6 M KCl, 250 mM sucrose, 3 mM MgCl2) or low salt (10 mM NaHCO3, 0.1 mM CaCl2). (bio.net)
  • Are there solutes to add (pyrophosphate, NaI, etc) that would allow easy and complete resuspension of insect cell microsomes? (bio.net)
  • cells
  • microsomes are not present in healthy, living cells. (wikipedia.org)
  • To examine the involvement of ER in catalase-induced BaP detoxification, we compared the level and distribution of XMEs, and the profile of BaP intermediates in the microsomes of wild-type and catalase transgenic endothelial cells. (sigmaaldrich.com)
  • proteins
  • Taken together, upregulation of phase II XMEs and CYP1 proteins, but not EH1 in the ER might be the mechanism by which overexpression of catalase reduces the levels of all the BaP metabolites, and enhances the ratio of BaP phenolic metabolites versus diol/diones in endothelial microsomes. (sigmaaldrich.com)
  • cell
  • Has anyone made ER microsomes from cell culture (specifically Cos) which are competent for Ca release measurements? (bio.net)
  • Low salt protocol: For preparation of Sf21 cell microsomes, the washed cell pellets were resuspended in 25 ml of medium containing 10 mM NaHCO3 and 0.2 mM CaCl2, supplemented with the protease inhibitors aprotinin (1 µg/ml), leupeptin (0.2 µg/ml), pepstatin A (1 µg/ml), and Pefabloc SC (0.1 mM). (bio.net)