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  • specimen
  • When these plane waves (parallel wave fronts) hit the phase object 'O' (located in the object plane labeled '2'), some of this light is diffracted (and/or refracted) while moving through the specimen. (microscopy-uk.org.uk)
  • Assuming that the specimen does not significantly alter the amplitudes of the incoming wavefronts but mainly changes phase relations with respect to the 'unperturbed' wavefronts, newly generated spherical wave fronts that are retarded by 90° (λ/4) emanate from 'O' (see the purple area that contains now 'unperturbed' plane waves and spherical wave fronts). (microscopy-uk.org.uk)
  • DIC microscopy, introduced in the late 1960s, has been popular in biomedical research because it highlights edges of specimen structural detail, provides high-resolution optical sections of thick specimens including tissue cells, eggs, and embryos and does not suffer from the phase halos typical of phase-contrast images. (jove.com)
  • In all forms of optical microscopy, the specimen scatters light through processes that include diffraction, refraction, reflection, and absorption. (fsu.edu)
  • We describe a novel microscopy technique for quantitative phase-contrast imaging of a transparent specimen. (osapublishing.org)
  • The technique is based on depth-resolved phase information provided by common path spectral-domain optical coherence tomography and can measure minute phase variations caused by changes in refractive index and thickness inside the specimen. (osapublishing.org)
  • Phase-contrast microscopy is an optical microscopy technique that converts phase shifts in light passing through a transparent specimen to brightness changes in the image. (microbenotes.com)
  • Wavefronts passing through the annulus illuminate the specimen and either pass through undeviated or are diffracted and retarded in phase by structures and phase gradients present in the specimen. (microbenotes.com)
  • tumor
  • The obtained results demonstrate exemplarily for two tumor cell lines that from DHM phase contrast images of fixed cells in phosphate buffer saline (PBS) cell thickness values are obtained which are comparable to living cells. (spie.org)
  • We demonstrate efficient and specific killing of tumor cells by enzymatic cytotoxicity assays, flow cytometry, as well as phase contrast microscopy. (igem.org)
  • Tomography
  • X-ray phase-contrast imaging and tomography make use of the refraction of X-rays by the sample in image formation. (mdpi.com)
  • In the context of materials science, X-ray phase-contrast imaging and tomography have particular value in the 2D and 3D characterization of low-density materials, the detection of cracks and voids and the analysis of composites and multiphase materials where the different components have similar X-ray attenuation coefficients. (mdpi.com)
  • Here we review the use of phase-contrast imaging and tomography for a wide variety of materials science characterization problems using both synchrotron and laboratory sources and further demonstrate the particular benefits of phase contrast in the laboratory setting with a series of case studies. (mdpi.com)
  • proportional
  • Therefore, an in-line phase-shifting digital holography system can be more useful in the precision shape measurement of transparent objects whose thickness or height profiles are proportional to the phase profiles of their complex images. (spiedigitallibrary.org)
  • images
  • 6 , 7 , 8 The object image reconstructed from this complex field is free from zero-order and the twin images and can have better phase resolution than can be obtained with an off-axis system. (spiedigitallibrary.org)
  • A second aim of the study was to identify subcellular components in DHM phase contrast images. (spie.org)
  • Recent studies at high magnetic fields using the phase of gradient-echo MR images have shown the ability to unveil cortical substructure in the human brain. (epfl.ch)
  • Phase images revealed, often more clearly than the corresponding magnitude images, hippocampal fields, cortical layers (e. g. layer 4), cerebellar layers (molecular and granule cell layers) and small white matter structures present in the striatum and septal nucleus. (epfl.ch)
  • The contrast of the phase images depended in part on the orientation of anatomical structures relative to the magnetic field, consistent with bulk susceptibility variations between tissues. (epfl.ch)
  • Find and compare the best bioinformatics software for tracking animal behaviors on time series phase contrast microscopy acquired images. (omictools.com)
  • rear focal plane
  • Undeviated and diffracted light collected by the objective is segregated at the rear focal plane by a phase plate and focused at the intermediate image plane to form the final phase contrast image observed in the eyepieces. (microbenotes.com)
  • particles
  • For example, current methods for characterizing dry material using microscopy can reveal general morphology and particle size, but cannot determine whether material consists of solid particles or clusters, or whether material will deagglomerate in liquids such as lung surfactant. (biomedcentral.com)
  • Imaging
  • The concept is based on the assumption that with the use of a high transverse imaging speed (of the order of several kilohertz), phase changes owing to jitter within the interferometer (or sample motion) can be separated from the phase changes introduced by the sample structure. (pubmedcentralcanada.ca)
  • To investigate the contrast mechanisms in phase imaging, this study extends, for the first time, phase imaging to the rodent brain. (epfl.ch)
  • Phase-contrast imaging highlights edges and internal boundaries of a sample and is thus complementary to absorption contrast, which is more sensitive to the bulk of the sample. (mdpi.com)
  • Biology
  • Our new phase contrast system is of interest to research institutions and universities that focus on structural biology first and foremost, but it is also useful to companies in the polymer and pharmaceuticals industries, which have to deal with similar contrast issues," explains Joerg Wamser, Director of KonTEM GmbH. (max-planck-innovation.de)