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  • optical
  • Confocal microscopy provides the capacity for direct, noninvasive, serial optical sectioning of intact, thick, living specimens with a minimum of sample preparation as well as a marginal improvement in lateral resolution. (wikipedia.org)
  • For an optical cavity consisting of two mirrors, confocal means that they share their foci. (wikipedia.org)
  • Many different techniques for optical sectioning are used and several microscopy techniques are specifically designed to improve the quality of optical sectioning. (wikipedia.org)
  • Good optical sectioning, often referred to as good depth or z resolution, is popular in modern microscopy as it allows the three-dimensional reconstruction of a sample from images captured at different focal planes. (wikipedia.org)
  • In comparison, the lateral resolution can be approximated as: D x = D y = 0.61 λ N A {\displaystyle D_{x}=D_{y}={\frac {0.61\lambda }{\mathrm {NA} }}} Beyond increasing numerical aperture, there are few techniques available to improve optical sectioning in bright-field light microscopy. (wikipedia.org)
  • Confocal microscopy, another optical technique, typically penetrates less deeply into the sample but with higher resolution. (wikipedia.org)
  • There are three well-known branches of microscopy: optical, electron, and scanning probe microscopy. (wikipedia.org)
  • The field of microscopy (optical microscopy) has its roots in the 17th-century Dutch Republic, with some notable representatives such as Zacharias Janssen, Hans Lippershey, Jan Swammerdam, and Antonie van Leeuwenhoek. (wikipedia.org)
  • Optical or light microscopy involves passing visible light transmitted through or reflected from the sample through a single lens or multiple lenses to allow a magnified view of the sample. (wikipedia.org)
  • tissue
  • Confocal microscopy has now become an important research tool, with a large number of new fluorescent dyes becoming available in the past few years, for probing your pet structure or molecule within fixed or living cell or tissue sampies. (waterstones.com)
  • examination
  • Besides routine ophthalmologic examination, anterior segment photography and confocal microscopy with cornea Rostoch module attached to HRT II (Heidelberg Engineering GmbH, Heidelberg, Germany) were performed. (hindawi.com)
  • high-resolution
  • Confocal microscopy provides high-resolution, high-contrast in vivo images and is a powerful tool for studying the ultrastructure of the cell, its molecular components, and their functions. (hindawi.com)
  • It allows an endoscope to perform in situ, high-resolution fluorescence microscopy imaging in real time, at the cellular and subcellular levels, via an instrument small and flexible enough to reach all the way into the tiny alveoli in the lungs. (photonics.com)
  • Observation
  • In fluorescence microscopy, colocalization refers to observation of the spatial overlap between two (or more) different fluorescent labels, each having a separate emission wavelength, to see if the different "targets" are located in the same area of the cell or very near to one another. (wikipedia.org)