• Besides routine ophthalmologic examination, anterior segment photography and confocal microscopy with cornea Rostoch module attached to HRT II (Heidelberg Engineering GmbH, Heidelberg, Germany) were performed. (hindawi.com)
  • The module consists of a monochrome laser radiation source which avoids chromatic aberrations and provides extremely sharp images and a high-powered lens that allows the operator to change the confocal plane within the cornea to capture images at different depths without losing sharpness [ 4 ]. (hindawi.com)
  • When imaging a sample in color confocal microscopy, this defect results in different colors of excitation illumination being focused to different points in the sample and different colors of emission being collected from different points in the sample. (microscopyu.com)
  • Confocal microscopy has now become an important research tool, with a large number of new fluorescent dyes becoming available in the past few years, for probing your pet structure or molecule within fixed or living cell or tissue sampies. (waterstones.com)
  • These optics allow Biomedical researchers to push the boundaries of microscopy, imaging deeper into tissue samples to determine their nature and structure. (lambdaphoto.co.uk)
  • They concisely describe all the key stages of confocal imaging-from tissue sampling methods, through the staining process, to the manipulation, presentation, and publication of the realized image. (springer.com)
  • Confocal microscopy as a technique is there to take high-resolution fluorescent images of your cells, your tissues and anything else that you may be happening to use as a biological specimen, and it does this by eliminating out-of-focus slides. (abcam.com)
  • Fluorescence emission gathered from the specimen is filtered through a confocal pinhole aperture to reject light that originates from regions removed from the focal plane. (zeiss.com)
  • The confocal was funded through a National Science Foundation Major Research Instrumentation (MRI) grant (#0618719). (unh.edu)
  • As compared to conventional electron microscopy (TEM, STEM, SEM), SCEM offers 3-dimensional imaging. (wikipedia.org)
  • The requirements for accurate color imaging have been further increased by the development of colorimetric methods of quantitative microscopy, such as fluorescence ratio measurements of ion concentrations. (microscopyu.com)
  • Quantum correlations from photon antibunching enhance the resolution of image scanning microscopy in biological imaging by twofold, four times beyond the diffraction limit. (nature.com)
  • Live cell microscopy has been my tool of preference to answer many biological research questions ever since. (tufts.edu)
  • Spectral phasor analysis allows unmixing fluorescence microscopy images, but it requires user involvement and has a limited number of labels that can be analyzed and displayed. (nature.com)
  • By eliminating signals that originate from outside the focal plane, confocal microscopy provides the ability to acquire high-resolution, optically sectioned images from within a thick sample or to reduce background fluorescence from thin cultures. (thorlabs.com)
  • Confocal microscopy provides high-resolution, high-contrast in vivo images and is a powerful tool for studying the ultrastructure of the cell, its molecular components, and their functions. (hindawi.com)
  • Linear lobe deconvolution as well as nonlinear restoration of 4Pi confocal raw data with their point-spread functions (PSF's) leads to almost-identical images, irrespective of whether the 4Pi confocal PSF relies on constructive or destructive interference. (osapublishing.org)
  • Moreover, restoration of 4Pi confocal images of the same object with alternate phases is introduced as a powerful test for (nonlinear) image restoration. (osapublishing.org)
  • The powerful hands-on methods collected in Confocal Microscopy Methods and Protocols will help even the novice to produce first-class cover-quality confocal images. (springer.com)
  • This is clearly a problem for any researcher trying to use color confocal microscopy to determine the relative distribution of multiple probes. (microscopyu.com)
  • In Confocal Microscopy Methods and Protocols, Stephen Paddock and a highly skilled panel of experts lead the researcher using confocal techniques from the bench top, through the imaging process, to the journal page. (springer.com)
  • Confocal microscopy not only supports diagnosis in ocular argyrosis, but also demonstrates the intensity of the deposition in these patients. (hindawi.com)
  • High-contrast round bodies suggestive of Acanthamoeba cysts, as previously described, and irregular forms suggestive of Acanthamoeba trophozoites were found by tandem scanning confocal microscopy. (nih.gov)
  • Additionally, we showed conclusively that under certain circumstances (that is, corneal scarring) tandem scanning confocal microscopy can resolve the double-walled structure of the Acanthamoeba ectocyst surrounding the endocyst. (nih.gov)
  • Confocal microscopy is a very helpful tool for the evaluation of corneal grafts and it allows us to evaluate its characteristics through time, letting us see the evolution and in some way predict the possible future complications. (arvojournals.org)
  • Every Thorlabs confocal upgrade features either a galvo-resonant or galvo-galvo scan head. (thorlabs.com)