• We will explore true force and displacement feedback control modes and cover applications from soft polymers to hard ceramics. (bruker.com)
  • Applications of Atomic Force Microscopy (AFM) are ubiquitous in all bioscience areas that involve molecules (nucleic acids, lipids, proteins…), cells (living cells in culture, bacteria…), tissues (bone, cartilage, dentine, cornea…), polymers (biopolymers such as collagen) and other materials. (nanohub.org)
  • This can be applied to perform force spectroscopy, to measure the mechanical properties of the sample, such as the sample's Young's modulus, a measure of stiffness. (wikipedia.org)
  • Over the last few years there has been significant progress in our understanding of the role played by the substrate stiffness in neuronal growth, of the cell-substrate adhesion forces, of the generation of traction forces during axonal elongation, and of the relationships between the neuron soma elastic properties and its health. (mdpi.com)
  • The particular capabilities of the Atomic Force Microscope (AFM), such as high spatial resolution, high degree of control over the magnitude and orientation of the applied forces, minimal sample damage, and the ability to image and interact with cells in physiologically relevant conditions make this technique particularly suitable for measuring mechanical properties of living neuronal cells. (mdpi.com)
  • Lastly, adaptations can allow an atomic force microscope to determine the chemical make-up, conductivity, electric field, magnetic structure, elasticity, and work function of a sample surface, while SEM can ascertain chemical make-up alone. (azonano.com)
  • Over the last decade, atomic force microscopy (AFM) has been proven to be a powerful tool for the analysis of morphological aspects of cell structures at nanometer scale resolution and more importantly their rheological properties in living cells ( 12 , 13 ). (pnas.org)
  • Controlled forces have also been used to precisely manipulate the cells under study. (mdpi.com)
  • The Perkins group continues to extend the performance of its unique Atomic Force Microscope (AFM) technology, revealing for the first time a dozen new short-lived intermediate states in the folding and unfolding of a membrane protein that controls the exchange of chemicals and ions into and out of living cells. (technologynetworks.com)
  • citation needed] Another potential problem with amplitude modulation is that a sudden change to a more repulsive (less attractive) force can shift the resonance past the drive frequency causing it to decrease again. (wikipedia.org)
  • In-situ atomic force microscopy study of beta-amyloid fibrillization. (nih.gov)
  • NEW YORK (GenomeWeb) - A study published online earlier this month in the Journal of the American Chemical Society provided details on a proposed single-molecule sequencing approach that brings together sequencing-by-synthesis with atomic force microscopy (AFM). (genomeweb.com)
  • By recording the drive amplitude during the scan (usually referred to as the damping channel as the need for a higher drive amplitude corresponds to more damping in the system) a complementary image is recorded showing only non-conservative forces. (wikipedia.org)
  • In constant height mode this will just lead to an image artefact, but in feedback mode the feedback will read this as a stronger attractive force, causing positive feedback until the feedback saturates. (wikipedia.org)
  • Because of its ability to image samples on an atomic scale, it has been vital to the advance of nanotechnology. (doitpoms.ac.uk)
  • To investigate the structure/function relationships of podosomes, soft lithography, atomic force microscopy (AFM), and correlative fluorescence microscopy were used to characterize podosome physical properties in macrophages differentiated from human blood monocytes. (pnas.org)