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  • miRNA
  • We surveyed expression profiles of microRNA (miRNA) in stage I NSCLC to identify patterns that might predict recurrence after surgical resection of this common deadly cancer. (aacrjournals.org)
  • Small RNA was extracted from deparaffinized and proteinase K-treated FFPE core tissue (20-40 mg) using the High Pure miRNA Isolation Kit (Roche). (aacrjournals.org)
  • A) Simplified microRNA (miRNA) biogenesis pathway. (pubmedcentralcanada.ca)
  • Ten FFPE samples from 6 cases of amyotrophic lateral sclerosis (ALS) and 4 neurologically normal controls were selected for miRNA analysis on the basis of the following criteria for RNA quality: (i) a postmortem interval of less than 6 hours, (ii) a formalin fixation time of less than 4 weeks, (iii) an RNA yield per sample of more than 500 ng, and (iv) sufficient quality of the RNA agarose gel image. (biomedcentral.com)
  • Many studies have reported that extracellular RNAs are transferred between cells and can exert regulatory function, however the biological contexts and mechanisms of miRNA transfer are not well understood. (washington.edu)
  • The pre-miRNA is a double-stranded hairpin structure of RNA with 60-70 bp in length [ 3 ], which can be further processed into matured miRNA by RNaseIII dicer, ultimately form RNA-induced silencing complex (RISC) after exported to cytoplasm by exportin-5 [ 4 ]. (hindawi.com)
  • MicroRNA profiles in peripheral blood samples (pre-challenge and 2 hours post-challenge) were measured by the NanoString nCounter assay to determine changes in miRNA levels as these asthmatic subjects underwent an allergen inhalation challenge. (biomedcentral.com)
  • molecules
  • Here, we characterized a set of small molecules that allow pharmacological manipulation of EZH2 and EZH1, which include UNC1999, a selective inhibitor of both enzymes, and UNC2400, an inactive analog compound useful for assessment of off-target effect. (jove.com)
  • therapeutics
  • RNA interference has revolutionized human functional genomics and therapeutics, but its impact on neuroscience research has been limited by the lack of simple and efficient methods to deliver oligonucleotides to primary neurons and the brain. (selectbiosciences.com)
  • Collectively, our study provides the detailed profiling for a set of chemicals to manipulate EZH2 and EZH1 and establishes specific enzymatic inhibition of PRC2-EZH2 and PRC2-EZH1 by small-molecule compounds as a novel therapeutics for MLL-rearranged leukemia. (jove.com)
  • Trang P, Weidhaas JB, Slack FJ (2008) MicroRNAs as potential cancer therapeutics. (springer.com)
  • binds
  • Vella MC, Choi EY, Lin SY, Reinert K, Slack FJ (2004) The C. elegans microRNA let-7 binds to imperfect let-7 complementary sites from the lin-41 3‵UTR. (springer.com)
  • antisense
  • LNCaP and PC3 human prostate cancer cells (American Type Culture Collection) were grown to 50% confluency and transfected with either microRNA precursor or antisense microRNA inhibitor (Ambion) at 100 nmol/L final concentration using Lipofectamine 2000 reagent (Invitrogen). (aacrjournals.org)
  • Circulating
  • Authoritative and practical, Circulating MicroRNAs: Methods and Protocols seeks to aid scientists in dealing with the recent advances of RNAi technology from the bench to the bedside. (springer.com)
  • cells
  • We have identified ncRNAs that selectively kill cancer cells or lead to their terminal differentiation and ncRNAs that selectively enhance cancer cell response to microtubule-targeting agents, revealing new vulnerabilities of cancer cells mediated by complex regulatory relationships between several classes of coding and non-coding RNAs. (selectbiosciences.com)
  • 2000
  • For example Professor Slack and colleagues were co-discoverers of the second known microRNA, let-7 (Reinhart et al Nature, 2000), and the co-discoverers of the first known human microRNA (Pasquinelli et al. (selectbiosciences.com)
  • function
  • To test the hypothesis that NSF and dysbindin/BLOC-1 participate in a pathway-regulating synaptic function, the role for NSF was studied in dysbindin/BLOC-1-dependent synaptic homeostatic plasticity in Drosophila. (sdbonline.org)
  • Through an adenovirus-mediated gain- and loss-of-function study, we found that miR-451 negatively regulates hepatic gluconeogenesis and blood glucose levels in normal mice and identified glycerol kinase (Gyk) as a direct target of miR-451. (diabetesjournals.org)
  • 2016
  • 2016. "MicroRNAs: Key Regulators in the Central Nervous System and Their Implication in Neurological Diseases. (mdpi.com)
  • FFPE
  • In the present study, we isolated RNAs from archived FFPE brain specimens of postmortem cases of ALS and neurologically normal controls. (biomedcentral.com)