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  • mechanisms
  • In humans, this microRNA is involved in the mechanisms of immunity, and in many different cancers (see below) it was found to be expressed at a particularly low level. (wikipedia.org)
  • miRNA
  • One strand functions as the mature miRNA and is incorporated into the RNA-Induced Silencing Complex (RISC) to target mRNAs for cleavage in a sequence-specific manner. (biomedcentral.com)
  • MicroRNA (miRNA)-mediated gene regulation contributes to liver pathophysiology, including hepatic stellate cell (HSC) activation and fibrosis progression. (springer.com)
  • miRNAs are precisely regulated and characteristic patterns of miRNA expression appear during brain development and neuronal differentiation in vitro ( 9 - 11 ). (aacrjournals.org)
  • Total RNA from human tissue samples and cell lines was isolated with Trizol reagent (Invitrogen, Carlsbad, CA). Oligonucleotide arrays were printed with tri-mer oligonucleotide probes (antisense to miRNAs) specific for 180 human and mouse miRNAs on GeneScreen Plus (NEN) membranes, and miRNA expression profiling was done and analyzed as described previously ( 9 ). (aacrjournals.org)
  • Once assembled into the RNA-induced silencing complex (RISC), the miRNA acts on its target by binding to complementary sequences present in the 3 ′ untranslated regions (UTR) of the target mRNA [ 6 ]. (pubmedcentralcanada.ca)
  • In the cytoplasm, another RNase III enzyme, Dicer, cuts the pre-miRNA to generate the mature microRNA as part of a short RNA duplex. (biologists.org)
  • Numerous miRNA regulates nuclear receptor ERa under the influence of estradiol (E2) such as miR- 101, miR-21 whereas miR145, miR-29a, miR-206, let-7 potentiates ERa proliferating activity. (jscholaronline.org)
  • Small mature RNA molecule produces over two steps such as formation of long hairpin pre-miRNA and RNA-induced silencing complex (RISC) contains dsRNA binding proteins including protein kinase RNA activator (PACT), transactivation response RNA binding protein (TRBP) process into mature miRNA[ 13 ]. (jscholaronline.org)
  • If you still need convincing, check out the back-to-back microRNA (miRNA) papers in yesterday's Neuron. (alzforum.org)
  • Important for understanding the functional roles of miRNAs is the ability to predict the messenger RNA (mRNA) targets most responsive to each miRNA. (springer.com)
  • siRNA
  • In vitro experiments, myocardial cells were isolated and cultured, an oxygen-glucose deprivation (OGD) model was established to mimicking the ischemic condition, the relationship between miR-298 and BAX was verified using luciferase reporter vector, lentivirus and small-interfering RNA (siRNA) in BAX. (europeanreview.org)
  • small RNA libraries
  • We used Illumina deep sequencing technology to sequence four small RNA libraries that were constructed from the inflorescences of rice plants that were grown under control condition and drought, cold, or salt stress. (biomedcentral.com)
  • hairpin
  • These effects are blocked by short hairpin RNAs specifically targeting the LepRb and are absent in cultures from db/db mice. (sigmaaldrich.com)
  • complementary
  • First, is binding of the AGO2-FXR1 complex, which activates translation, directed by a microRNA complementary to the ARE? (sciencemag.org)
  • A bioinformatic screen identified five microRNAs in miRBASE with seed regions complementary to the TNFα ARE, not including miR16 ( 5 )[see supporting online material (SOM) text]. (sciencemag.org)
  • MicroRNA sequences: wild-type miR369-3, seed mutant miRseedmtR369-3 (complementary to mtAREseed1 and mtAREseed2), and miRmt369-3 (complementary to mtARE). (sciencemag.org)
  • transcriptional
  • These factors regulate each other at the transcriptional level in embryos, and their interactions vary depending on tissue type. (plantphysiol.org)
  • Finally, the involvement of microRNAs in transcriptional gene silencing (TGS), which has been observed in plants, remains an intriguing possibility ( Baulcombe, 2004 ). (biologists.org)
  • processes
  • MicroRNAs are involved in many biological processes and diseases, particularly multifactorial diseases, providing an excellent tool with which to research the mechanisms of these diseases. (frontiersin.org)
  • In this review, we will highlight the effect of microRNAs in different pathological processes throughout AD progression. (frontiersin.org)
  • genomics
  • Blood-brain barrier (BBB) dysfunction is a major hallmark of many neurological diseases, including multiple sclerosis (MS). Using a genomics approach, we defined a microRNA signature that is diminished at the BBB of MS patients. (jneurosci.org)
  • progression
  • Thus the mechanisms that regulate expression of NMI are of potential interest for understanding the etiology of breast tumor progression and metastasis. (biomedcentral.com)
  • We further outline how these miRNAs could be regulated to influence cell cycle progression in stem cells as a potential clinical application. (springer.com)
  • endothelial cell
  • Here, we tested whether miR-146a regulates in vitro angiogenic endothelial cell (EC) behaviors, as well as perfusion recovery, arteriogenesis, and angiogenesis in response to femoral arterial ligation (FAL) in vivo . (frontiersin.org)
  • target
  • Looking to find a promising target for an individual microRNA Christi. (bio-medicine.org)
  • Looking to find a promising target for an individual microRNA, Christine Mayr, a postdoctoral researcher in the Bartel lab, picked Hmga2, a gene that is defective in a wide range of tumors. (bio-medicine.org)
  • Leptin also increases expression of microRNA-132 (miR132), a well-known CREB target, which is also required for leptin-induced synaptogenesis. (sigmaaldrich.com)
  • Tumor necrosis factor ligand superfamily member 14 ( TNFSF14 ) was a microRNA target gene significantly decreased in MS. The differential expression of mir-494 , mir-197 and the predicted microRNA target gene TNFSF14 was verified by real-time PCR and ELISA. (biomedcentral.com)
  • Moreover
  • Moreover, microRNAs found in mammals regulate over a third of the human genome, as shown in a 2005 study by the lab of Whitehead Member and Howard Hughes Medical Institute Investigator David Bartel and colleagues. (bio-medicine.org)
  • Mechanisms
  • In humans, this microRNA is involved in the mechanisms of immunity, and in many different cancers (see below) it was found to be expressed at a particularly low level. (wikipedia.org)