Microorganisms laccase. Medical search. Frequent questions

In this study, ligninolytic activity of F. virguliforme was analyzed by the catalyzed release of 14CO2 from purified 14C-labeled Klason lignin, the degradation of polymeric aromatic dyes in culture (a method commonly used to test the ligninolytic capacity of microorganisms), and the production of the major fungal lignin degrading enzymes, laccase and lignin peroxidase. (usda.gov)
As of 2007[update], enzymes including laccase were under exploration for their applications in discharge printing. (wikipedia.org)
With this source laccases are one of enzymes with high amount of literatures could be observed. (scialert.net)
These unique properties made laccases to be promising enzymes for bioremediation. (scialert.net)
However, many substrates of laccases are not oxidized due to the enzymes have relatively low redox potentials. (scialert.net)
There are certain groups of lignin degrading fungi which are capable of synthesizing non-specific enzymes like laccase, lignin peroxidase and manganese peroxidase ( Khammuang and Sarnthima, 2009 ). (scialert.net)
Based on source, the specialty enzymes market is segmented into microorganism, plant, and animal. (marketsandmarkets.com)
The microorganism segment is projected to witness the highest growth during the forecast period, as enzymes obtained from microbial sources lead to low production costs. (marketsandmarkets.com)
In addition, as enzymes obtained from microbial sources can be cultured in large quantities in a short period, microorganisms are the primary source of specialty enzymes. (marketsandmarkets.com)
There are also several other microbial enzymes that are believed to be involved in lignin biodegradation, such as manganese peroxidase, laccase. (bioplasticsnews.com)
In 2016, researchers successfully added a laccase, or lignin-degrading enzymes, from the aerobic bacterium Thermobifida fusca into a designer cellulosome, a multienzyme complex structure commonly found in anaerobic bacteria. (nutritionmodels.com)
According to the application, the garden waste particles are used for culturing the mushrooms, and the complex structure of lignin in the garden waste is destroyed by using extracellular enzymes such as laccase and peroxidase secreted by hyphae, so that organic matter in mushroom residues obtained after the mushrooms are harvested is more easily degraded by aerobic microorganisms. (patsnap.com)
This chapter also emphasizes the usage of some prominent microorganisms, their secreted enzymes and their proposed mechanisms of action involved with the degradation of the waste products. (preprints.org)
The specific textile enzymes used are amylases, catalase, and laccase for removing the starch, degrading excess hydrogen peroxide, textile bleaching, and lignin-degrading. (sylicglobal.com)
Examples of bioprospecting products used in bioremediation include Coriolopsis gallica - and Phanerochaete chrysosporium -derived laccase enzymes, used for treating beer factory wastewater and for dechlorinating and decolorizing paper mill effluent . (alquds.edu)

Results showed that the laccase and lignin peroxidase activities and levels of decolorization of aromatic polymeric dyes (Poly R-478 and Remazol Brilliant Blue R) by F. virguliforme were intermediate or even greater than that found in our tests with two known lignin-degrading fungi, Polyporus tulipifera and Schizophyllum commune. (usda.gov)
This enzyme firstly found in Japanese lacquer tree, Rhus vernicifera and not long after that laccases from fungi has been reported, especially in white-rot fungi. (scialert.net)
Therefore, it would be a safe and cheap strategy for the commercial production of laccase by filamentous fungi. (bvsalud.org)
Microorganisms capable of catabolizing lignin-derived ar- carbohydrate and protein components of the organic matter omatics are essentially fungi and bacteria (Bugg et al. (lu.se)
2005). These fungi produce sever- lected in the present study by enrichment culture, using kraft al extracellular peroxidases, laccases, and additional oxidative lignin and kraft pulping lignin-enriched streams. (lu.se)
Previous studies have shown that many microorganisms (bacteria and fungi) can degrade xenobiotics. (japsonline.com)
This enzyme emerges as a key player in the biodegradation of GRMs within terrestrial ecosystems, as its release extends beyond fungi to several other microorganisms. (units.it)
Industrial and agricultural wastes as substrates for laccase production by white-rot fungi. (mushroomstudies.co)
White-rot fungi, Coriolus versicolor and Funalia trogii, produced laccase on media with diluted olive-oil mill wastewater and vinasse. (mushroomstudies.co)

Pilát causes white rot in wood and has been identified as an important fungal laccase producer. (bvsalud.org)
Moreover, adding different concentrations of copper to the fungal culture medium increased the production of laccase, and the highest amount of enzyme (92.04 u/l) was obtained with 3.5 mM of CuSO4 along with oak sawdust. (bvsalud.org)
Based on the results, the addition of host wood sawdust ("oak" in this work) and copper particles together stimulates the fungal growth and the laccase production during submerged cultivation of T. versicolor. (bvsalud.org)
Active fungal Laccase with optimum at neutral pH. (asa-enzyme.com)

Laccases (benzendiol: oxygen oxidoreductase, EC 1.10.3.2) are belong to polyphenol oxidase, catalyze the reduction of dioxygen to water while oxidize its substrates ( Baldrian, 2006 ). (scialert.net)
The laccase (EC 1.10.3.2) from Trametes versicolor was purchased from Fluka (specific activity 0.53 U mg -1 ) and was used without further purification. (scialert.net)

In the laccase-ABTS mediator system, the best molar ratio of dye/mediator was 1:10 and dye/enzyme ratio was 0.5 μmol U -1 . (scialert.net)
Sequential submerged cultivation with different induction media as inducers of ligninolytic enzyme production by Pleurotus ostreatus strains was assessed by measuring laccase activities. (ncsu.edu)
The laccase enzyme of different P. ostreatus strains in the different media exhibited large differences, and the wild strain YAASM 0568 exhibited enhanced production of laccase compared to other cultivated strains. (ncsu.edu)
PANI laccase showed the highest stability and activity, producing a power density of 38 ± 1.7 mW m−2 compared to 25.6 ± 2.1 mW m−2 for Nafion laccase, 14.7 ± 1.04 mW m−2 for Cu-Alg laccase, and 28 ± 0.98 mW m−2 for the freely suspended enzyme. (westminster.ac.uk)
There was 81% enzyme activity retained after 1 cycle (5 days) for PANI laccase compared to 69% for Nafion and 61.5% activity for Cu-alginate laccase and 23.8% activity retention for the freely suspended laccase compared to initial activity. (westminster.ac.uk)
Its ability to produce laccase enzyme in the presence of different plant substrates including sawdust and wood chips of oak, poplar, and pine was evaluated. (bvsalud.org)
The resulting recombinant enzyme was recovered in active and soluble form, exhibiting optimum copper-dependent laccase activity at 55 °C, pH 6.5 with syringaldazine substrate, retaining over 60% of its activity after 1 h at 55 and 60 °C. In addition, this thermophilic enzyme is not affected by common inhibitors SDS, NaCl and L-cysteine. (bvsalud.org)
This use of laccase may be an early example of the rapid application of this long-known enzyme in biofuel production and other industries, such as pulp and paper and crop biotechnology. (nutritionmodels.com)
Grapes affected by Botrytis are extremely difficult to transform as the presence of the enzyme laccase , synthesized by the fungus, favors oxidative reactions at the expense of numerous wine components, polyphenols in particular, leading to quality degradation . (biotecnologiebt.it)

MINIATURA 1 (NCN) (2017) „Ocena potencjału bioremediacyjnego lakazy Sinorhizobium meliloti" [Assessment of the bioremediation potential of Sinorhizobium meliloti laccase]. (umcs.pl)
Thus, as a promising alternative treatment, bioremediation by microorganisms may be a safer and cheaper alternative to removing amoxicillin from the environment. (japsonline.com)

Biomass, pH, H2O2 content and activity of laccase (Lac) and lignin peroxidase (LiP) in the culture media were monitored, along with the physicochemical changes of GO over time followed by TEM, Raman, XPS and TGA. (units.it)

These results suggest that hydroquinone produces oxidative stress in this microorganism. (uai.cl)

Laccases efficiency could be enhanced when the low molecular weight compounds are included as redox mediators. (scialert.net)
In the present study, laccase from Trametes versicolor was immobilized using three different approaches, i.e., crosslinking with electropolymerized polyaniline (PANI), entrapment in copper alginate beads (Cu-Alg), and encapsulation in Nafion micelles (Nafion), in the absence of redox mediators. (westminster.ac.uk)

However, the reactions catalyze by LiP or MnP need hydrogen peroxide as co-substrate, this substance is not necessary for laccases catalysis. (scialert.net)
Because microbial laccases have a broad substrate range, they can be used in biosensor technology to detect a wide range of organic compounds . (alquds.edu)
They also demonstrated the ability of microorganisms to selectively discriminate against 13C even when degrading an isolated simple substrate. (mushroomstudies.co)
Laccase substrate 2,6-dimethoxylphenol and others, such as syringaldazine, o-tolidine, and ABTS, were readily oxidized, among which the Km for syringaldazine was the highest. (ncsu.edu)

A bioinformatic search using local databases led to the identification of three putative multicopper oxidase sequences in the genome of this microorganism. (bvsalud.org)

Several methods of carbamate removal have been developed, such as: oxidation, adsorption, biodegradation with microorganisms and advanced oxidation processes (AOPs). (journalingeniar.org)

To investigate laccase production and activity in T. versicolor, the native isolate was collected from the host (Quercus castaneifolia) in the forests of Guilan province, northern Iran, and then purified and identified using the molecular marker. (bvsalud.org)

The normal laccase from fungus or bacterial contains four copper atoms per protein molecular, imparting a blue color. (ncsu.edu)

The current study explores the potential of laccase immobilized biocathode for dye decolourization in a microbial fuel cell. (westminster.ac.uk)

Here it is reported that a white laccase is produced by a white rot fungus Panus conchatus from its solid-state fermentation. (ncsu.edu)

Laccases are appealing biocatalysts for various industrial utilizations. (bvsalud.org)

The AWRI undertook a thorough review of laccase analysis and has recently developed a new assay. (awri.com.au)

This study reported the decolorization of hazardous xanthenes dye, Rhodamine B by the Laccase Mediator System (LMS). (scialert.net)
The optimum conditions for Rhodamine B decolorization were at pH 4.0-5.0 and temperature 35-40°C. The laccase-ABTS system could be a promising biotechnology developed for treatment of textile waste waters containing Rhodamine B. (scialert.net)
There has no report described about this dye decolorization by laccase. (scialert.net)
Therefore, this research aims to investigate the decolorization of Rhodamine B by laccase and Laccase-Mediator System (LMS). (scialert.net)

Addition of spent cotton stalks enhanced the laccase activity with a maximum after 12 d of cultivation. (mushroomstudies.co)

An unconventional material, alkali lignin, was used for the first time as an inducer for different strains to enhance laccase activity. (ncsu.edu)
The P. ostreatus strains secreted similar but relatively high levels of laccase activity when the induction media contained alkali lignin with or without glucose. (ncsu.edu)
ID17 is a gram-positive thermophilic bacterium isolated from Deception Island, Antarctica, which has shown to exhibit remarkable laccase activity in crude extract at high temperatures. (bvsalud.org)
The activity center of this laccase is Cu2FeZn, which lacks the typical type-1 blue copper color. (ncsu.edu)

Thus, the fabricated laccase@GA@YPO4â ¢HNSs could be presented as a novel, efficient, and recyclable heterogeneous biocatalyst for wastewater treatment and clean-up. (bvsalud.org)
For example, laccase-containing electrodes are used to detect polyphenolic compounds in wine , and lignins and phenols in wastewater . (alquds.edu)

The polyacrylamide gel electrophoresis of purified laccase showed a main polypeptide with a molecular weight of about 60 kDa. (ncsu.edu)

The results showed that adding the wood to the culture medium increased laccase production, and among these, oak sawdust had the greatest effect, a 1.7-fold increase from that in the control (4.8 u/l vs. 2.8 u/l). (bvsalud.org)
Poly-3-hydroxybutyrate (PHB), the most abundant type of polyhydroxyalkanoates (PHA) is synthesized inside a variety of microorganisms as a primary candidate for industrial PHB production. (springeropen.com)

Ces dernières années, les méthodes biologiques, telles que la fermentation à l'état solide, ont acquis une grande importance pour la biorestauration de molécules complexes en raison de leur efficacité et de leur faible impact environnemental. (ac.be)

Winemakers contacted the AWRI's Winemaking and Extension Services Team for technical advice and assistance on processing Botrytis-affected fruit and winemaking strategies to best manage the negative effects of laccase. (awri.com.au)

Antibacterial agents made of silver ions, an antibacterial material or substance, can inhibit odors caused by the growth of microorganisms. (sylicglobal.com)

C. antarcticus produced the highest laccase and Mn-peroxldase activities (33.0 and 1.6 U/g dry solid). (scielo.org.ar)

Nutrient cycling within terrestrial ecosystems is mostly performed via the activities of soil-borne microorganisms [ 1 ]. (hindawi.com)

One example of such progress was the ability to manipulate microorganisms genomically to produce biofuel more efficiently and in a more sustainable way. (nutritionmodels.com)

In the present study, yttrium (Y3+) phosphate was applied for the synthesis of hybrid nanoparticles (HNPs) consisting of laccase as the green catalyst. (bvsalud.org)
Sequence analysis revealed that one of those sequences contains the four-essential copper-binding sites present in other well characterized laccases. (bvsalud.org)
Microorganisms present in the compost samples were se- scrutinized (Martínez et al. (lu.se)

Laccase is a kind of polyphenol oxidase having potential in applications for pulp bleaching, waste water treatment in mills, and removal of phenols in the food industry. (ncsu.edu)

On the other hand, it was observed that hydroquinone, but not H 2 O 2 , affects Mn-dependent peroxide and laccase transcripts levels. (uai.cl)
On the other hand, it was observed that hydroquinone, but not H2O2, affects Mn-dependent peroxide and laccase transcripts levels. (uai.cl)

In addition, MCO and laccase will be applied in combination with silica or other metal oxide-forming proteins (recombinant silicatein and silintaphin-1) to generate nanoparticles containing multiple shells of various materials, which can be doped with fluorescent dyes and proteins during their formation at mild conditions. (irb.hr)

In response to the issues encountered it became obvious that there was a requirement to develop a laccase method targeted at Australian conditions. (awri.com.au)

The microorganism segment is projected to witness significant growth during the forecast period. (marketsandmarkets.com)

Organisms10

Chemicals and Drugs9

Technology, Industry, Agriculture1

Information Science1

Health Care1

Enzymes15

Fungi9

Fungal4

1.10.3.22

Enzyme9

Bioremediation2

Biomass1

Oxidative1

Redox mediators2

Substrate4

Multicopper oxidase1

Oxidation1

Versicolor1

Bacterial1

Microbial1

Fungus1

Biocatalysts1

Assay1

Decolorization4

Cultivation1

Activity4

Wastewater2

Molecular weight1

Production2

Fermentation1

Strategies1

Substance1

Produced the highest1

Activities1

Ability1

Present3

Potential1

Levels2

Addition1

Method1

Growth1