Loading...
  • 1968
  • An important development came when H.O. Smith, K.W. Wilcox, and T.J. Kelley, working at Johns Hopkins University in 1968, isolated and characterized the first restriction nuclease whose functioning depended on a specific DNA nucleotide sequence. (wikipedia.org)
  • Buffer
  • Nuclease activity is not detected after incubation of 1 µg lambda DNA with 50 units of this enzyme for 10 minutes at 37°C in the reaction enzyme buffer (1 x H buffer), as judged from the agarose gel electrophoresis pattern. (clontech.com)
  • sensitivity
  • Use of a combination of selective ox-bile blood cell lysis and removal of human DNA with micrococcal nuclease significantly improves PCR sensitivity and offers a better option for improved typhoid PCR assays directly using clinical specimens in diagnosis of this globally important infection disease which we believe could be of importance in improving clinical care and providing effective evaluation of novel vaccines. (biomedcentral.com)
  • molecular
  • Nucleases are also extensively used in molecular cloning. (wikipedia.org)
  • They found that the HindII enzyme always cuts directly in the center of this sequence (between the 3rd and 4th base pairs) Most nucleases are classified by the Enzyme Commission number of the "Nomenclature Committee of the International Union of Biochemistry and Molecular Biology" as hydrolases (EC-number 3). (wikipedia.org)
  • Cells
  • In contrast to the existing model for tower development in S. aureus, the results of this study suggest the presence of a Sae-controlled nuclease-mediated exodus of biofilm cells that is required for the development of tower structures. (nih.gov)
  • Blood samples were mixed with ox bile for selective lysis of human blood cells and the released human DNA was then digested with addition of bile resistant micrococcal nuclease. (biomedcentral.com)