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  • Composition
  • The significance of our research is in identifying the microbial composition of biofilms formed on dishwasher rubber seals, how diverse abiotic conditions affect microbiota, and which key microbial members were represented in early colonization and contamination of dishwashers, as these appliances can present a source of domestic cross-contamination that leads to broader medical impacts. (asm.org)
  • We describe a method for the development of multi-species oral biofilms that are comparable, with respect to species composition, to supragingival dental plaque, under conditions similar to the human oral cavity. (jove.com)
  • The species composition of microbial populations in termite guts as well as key genes involved in various biochemical processes has been explored using molecular techniques (Kudo et al. (jove.com)
  • However, the model predicted that community composition and short chain fatty acid levels could be strongly affected only over small ranges of byproduct uptake rates, indicating a possible lack of flexibility in our cross-feeding mechanism. (mdpi.com)
  • Determining which factors control biofilm composition is therefore desirable when developing novel clinical treatments to combat caries, but is also challenging due to the system complexity and the existence of multiple bacterial species performing similar functions. (jove.com)
  • Parameter sensitivity was quantified by individually varying the model parameters against putative experimental measures, suggesting non-lethal interventions that can favourably modulate biofilm composition. (jove.com)
  • Biofilms are highly dynamic, organized and structured communities of microbial cells enmeshed in an extracellular matrix of variable density and composition 1, 2 . (jove.com)
  • however biotic feedbacks such as plant-soil feedbacks (PSF) have large effects on plant performance, and create environmental heterogeneity that depends on the community composition. (jove.com)
  • Theory that incorporates environmental heterogeneity influenced by the assembling community and additional empirical work is needed to determine when heterogeneity intrinsic to the assembling community will result in different assembly outcomes compared with heterogeneity extrinsic to the community composition. (jove.com)
  • populations
  • Here we employ agent-based mathematical modelling to simulate a biofilm consisting of two competing, distinct types of bacterial populations, each parameterised by their nutrient uptake and aciduricity, periodically subjected to an acid challenge resulting from the metabolism of dietary carbohydrates. (jove.com)
  • multi-species
  • Specifically, this methods article will describe how a commercially available microfluidic system can be adapted to facilitate the development of multi-species oral biofilms derived from and grown within pooled saliva. (jove.com)
  • Collectively
  • Collectively, these results indicate that P. gingivalis can assemble into a heterotypic community with F. nucleatum and S. gordonii, and that a community lifestyle provides physiologic support for P. gingivalis. (nih.gov)
  • Collectively, these findings indicate that InlJ-Als3-dependent binding facilitates interdomain community development between C. albicans and P. gingivalis and that P. gingivalis has the potential for increased virulence within such communities. (asm.org)
  • metagenomics
  • Considering that the use of new bacterial community analysis techniques ( e.g., microbiomics, metagenomics) is becoming more prevalent in the agricultural and environmental sciences and many environmental samples within these disciplines can be physiochemically and microbiologically unique ( e.g., fecal and litter/bedding samples from the poultry production spectrum), appropriate and effective DNA extraction methods need to be carefully chosen. (jove.com)
  • microscopy
  • The application of advanced microscopy and molecular and electrochemical high-resolution methods has provided insights into the structural organization and function of biofilm communities. (nih.gov)
  • Appearance of the biofilm produced by a Haitian clinical rugose strain (altered biotype El Tor HC16R) differed from that of a typical El Tor rugose strain (N16961R) by confocal microscopy. (jove.com)
  • biochemical
  • Therefore, we have developed an analytical tool-box to facilitate biofilm analysis at structural, biochemical and molecular levels by combining commonly available and novel techniques with custom-made software for data analysis. (jove.com)
  • ecosystems
  • To obtain an in-depth understanding of animals, you will start with a general study of the biosciences, from cell biology and the study of insects, to whole organisms and species, communities and ecosystems. (southampton.ac.uk)
  • genes
  • Initial reductionism included zooming in from complex multispecies microbial communities to isolated genes from individual species. (pubmedcentralcanada.ca)
  • Transcriptional profiling by RNA sequencing (RNA-Seq) established that 57 genes were uniquely upregulated in an InlJ-dependent manner in P. gingivalis - C. albicans communities, with overrepresentation of those corresponding to 31 gene ontology terms, including those associated with growth and division. (asm.org)
  • abundance
  • Both Seine and Sedlo heterotrophic community (abundance and biomass) dominate during winter and summer months, representing 75% (Sedlo, July) to 86% (Seine, November) of the total plankton biomass. (jove.com)
  • deficient
  • Biofilms produced by mutants deficient in rhamnolipid synthesis do not maintain the noncolonized channels surrounding macrocolonies. (asm.org)
  • microorganisms
  • Thus, we propose that compatible solutes provided by other microorganisms may contribute to survival of V. cholerae in the marine environment through facilitation of osmoadaptation and biofilm development. (asm.org)
  • However, our research shows that persisting polyextremotolerant groups of microorganisms in household appliances are well established under these unfavorable conditions and supported by the biofilm mode of growth. (asm.org)
  • surfaces
  • Found all over the planet - from desert rocks to the surfaces of buildings - biofilms are an integral part of nature. (medicalnewstoday.com)
  • Community succession on corroding surfaces was assessed using Illumina MiSeq sequencing of 16S bacterial rRNA amplicons and Sanger sequencing of 16S universal rRNA clones. (jove.com)
  • nutrient
  • In much the same way as human communities, biofilms have highly specialized areas: some of them are responsible for nutrient recycling, while others produce new EPS components or send messages from one area of the biofilm to another. (medicalnewstoday.com)
  • Furthermore, a system that uses natural human saliva as the nutrient source, instead of artificial media, is particularly desirable in order to support the expression of cellular and biofilm-specific properties that mimic the in vivo communities. (jove.com)
  • The termite gut tract contains a dense and species-rich microbial population that assists in the degradation of lignocellulose predominantly into acetate, the key nutrient fueling termite metabolism (Odelson & Breznak, 1983). (jove.com)
  • We found that cross feeding allowed coexistence to be robustly maintained for large variations in biofilm thickness and nutrient levels. (mdpi.com)
  • clinical
  • Extensive clinical studies have indicated that the oral microbial flora is responsible for two major human diseases: dental caries (tooth decay) and periodontitis (gum disease) ( 28 , 119 , 128 , 148 , 188 ). (pubmedcentralcanada.ca)
  • Ninety-six semen samples collected were examined for bacterial communities, measuring seven clinical criteria for semen quality (semen volume, sperm concentration, motility, Kruger's strict morphology, antisperm antibody (IgA), Atypical, and leukocytes). (jove.com)
  • complex
  • Nevertheless, the complex circulation around both seamounts with the presence of different sources of mesoscale variability (e.g. presence of meddies, intrusion of African upwelling water) may have contributed to the different patterns of distribution, abundances and also changes observed in the microbial community. (jove.com)
  • Periodontitis ensues from the action of complex heterogeneous microbial communities that inhabit the subgingival compartment ( 6 ). (asm.org)
  • The gut microbiome is a highly complex microbial community that strongly impacts human health and disease. (mdpi.com)
  • novel
  • In comparison to the strict mechanical and enzymatic DNA extraction methods, this novel hybrid method provided the best overall combined performance when considering quantitative (using 16S rRNA qPCR) and qualitative (using microbiomics) estimates of the total bacterial communities when processing poultry feces and litter samples. (jove.com)
  • antibiotics
  • Interestingly, antibiotics can actually enter a biofilm in many instances, but the EPS actively shields its inhabitants from such compounds, promoting antibiotic resistance. (medicalnewstoday.com)
  • Such treatments include surgically removing the area that is infected with a biofilm or using high-velocity water sprays to physically disrupt the biofilm, often in combination with antibiotics. (medicalnewstoday.com)
  • organization
  • We also provide an experimental case showing how the tool-box can assist with biofilms analysis, data organization, integration and interpretation. (jove.com)
  • Understanding
  • Understanding the importance of PSF for plant community assembly necessitates understanding of the role of heterogeneity in PSF, in addition to mean PSF effects. (jove.com)
  • different
  • It appears that cellular properties such as growth differentiation, chemotaxis, and cell-to-cell signaling enable biofilm communities to organize structurally in response to the external conditions and the activities of the different biofilm members. (nih.gov)
  • The mosquito gut accommodates dynamic microbial communities across different stages of the insect's life cycle. (jove.com)
  • development
  • In fact, we have demonstrated that, in high-osmolarity medium, V. cholerae growth and biofilm development are enhanced by supplementation with either glycine betaine or spent media from other bacterial species. (asm.org)
  • We propose that the maintenance of biofilm architecture represents a previously unrecognized step in the development of these microbial communities. (asm.org)
  • As a priority habitat the nature of the impacts described on M. modiolus communities are important to the development of conservation management policy and indicators of condition in Marine Protected Areas (EU Habitats Directive) as well as indicators of Good Environmental Status under the European Union Marine Strategy Framework Directive. (jove.com)
  • Thus
  • Thus, straw addition can be used to improve and to stabilize the anaerobic process in substrates lacking biofilm-supporting structures. (doaj.org)
  • metabolic
  • We developed a biofilm metabolic model of a very simple gut microbiome community consisting of a representative bacteroidete ( Bacteroides thetaiotaomicron ), firmicute ( Faecalibacterium prausnitzii ) and proteobacterium ( Escherichia coli ) to investigate the putative role of metabolic byproduct cross feeding between species on community stability, robustness and flexibility. (mdpi.com)
  • investigate
  • Whole cell quantitative proteomics, along with mutant construction and analysis, were conducted to investigate how P. gingivalis adapts to this three species community. (nih.gov)
  • Here we present an analysis approach using next-generation sequencing technology and bioinformatics analysis to investigate the associations between bacterial communities and semen quality. (jove.com)
  • survival
  • Limiting factors, such as high temperatures, high and low pHs, high NaCl concentrations, presence of detergents, and shear force from water during washing cycles, define microbial survival in this extreme system. (asm.org)