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  • bacteria
  • We previously developed and validated a vortexing-sonication technique for detection of biofilm bacteria on the surface of explanted prosthetic joints. (hindawi.com)
  • Although this traditional way of culturing bacteria in liquid medium has been instrumental in the study of microbial pathogenesis and enlightening as to some of the amazing facets of microbial physiology, pure- culture planktonic growth is rarely how bacteria exist in nature. (scribd.com)
  • Biofilms of pathogenic bacteria and their role in chronization of infectious process. (springer.com)
  • Result: The Sg/So/Ss and Sg / Fn/Pg biofilms elicited significantly elevated levels of IL-1a compared to any of the amounts of planktonic challenge and showed synergistic stimulatory activity compared to that expected as simply an additive effect of the 3 individual bacteria. (umich.edu)
  • It is now well recognised that populations of bacteria from many Gram-positive and Gram-negative species cooperate and communicate to perform diverse social behaviours including swarming, toxin production and biofilm formation. (caister.com)
  • A total of 40 bacteria and 8 fungi could be tested for biofilm production. (springer.com)
  • In vitro adherence testing revealed that majority i.e. 16 (88.8 %) of the 18 biofilm positive bacteria were adherent to artificial surfaces. (springer.com)
  • Filamentous fungi, like bacteria were capable of forming biofilms, which could be one of the important virulence factors in determining the pathogenic potential of these organisms in causing chronic rhinosinusitis and orbital cellulitis. (springer.com)
  • By comparison, both bacteria resided in suspension and biofilm samples when soluble carboxymethyl cellulose was used. (iwaponline.com)
  • The protective dome of a biofilm shields bacteria from antibiotics. (medicalnewstoday.com)
  • When bacteria flock together and form a community, this is called a biofilm. (medicalnewstoday.com)
  • Biofilms can be made up of populations of the same bacteria or of communities, which, in turn, are made up of many different species, all living together under a protective dome. (medicalnewstoday.com)
  • Biofilm residence also affords the bacteria with antibiotic-resistant genes the ability to easily share these among their neighbors using a process called horizontal gene transfer . (medicalnewstoday.com)
  • Only nanomolar to micromolar concentrations of EIP and H 2 O 2 were required to impact biofilm formation or disruption, which are significantly lower concentrations than those causing bactericidal effects on planktonic bacteria. (gsu.edu)
  • The Pseudomonas Fluorescens SBW25 Wrinkly Spreader Biofilm requires attachment factor, Cellulose Fibre and LPS interactions to maintain Strength and Integrity Insert Name Course Tutor Institution Date Introduction Several bacteria have the ability to form huge assemblages on plant and animal surfaces and tissues, on biological sediments, detritus, soils and many other geological structures, as well as suspended flocs in water columns. (studentshare.net)
  • Transmission electron microscopy of these biofilms indicates that complex epilithic microbial communities developed, consisting of cyanobacteria and fungi symbiotically associated in a lichen, along with a consortium of free-living algae and gram-negative bacteria. (geoscienceworld.org)
  • Long-term effects of two antibiotics were studied on periphytic biofilms (communities of predominantly microalgae and bacteria), established in aquaria from the indigenous micro-biota found in the natural seawater in the Gullmar fjord on the Swedish west coast. (chalmers.se)
  • Ecology
  • The oral microbial ecology is comprised of hundreds of bacterial species that co-exist as multispecies biofilms throughout a range of ecological niches in the oral cavity. (umich.edu)
  • The methodology for understanding the microbial ecology of biofilms is now much more readily available and the capacity to manipulate biofilms is becoming an important feature of biotechnology. (unt.edu)
  • This paper describes the utility of CMEIAS (Center for Microbial Ecology Image Analysis System) computer-assisted microscopy to extract data from accurately segmented images that provide 63 different insights into the ecophysiology of microbial populations and communities within biofilms and other habitats. (mdpi.com)
  • A major challenge in microbial ecology is to develop reliable methods of computer-assisted microscopy that can analyze complex digital images of microorganisms at single cell resolution and reveal insights about their ecology in situ without cultivation. (mdpi.com)
  • In this study, electricity generation and the microbial ecology of cellulose-fed MFCs were analyzed using a defined co-culture of Clostridium cellulolyticum and Geobacter sulfurreducens . (iwaponline.com)
  • The spectacular rise of massively parallel sequencing (next generation sequencing, NGS) technologies in mid 2000s initiated a renaissance in microbial ecology by allowing the in situ investigation of environmental samples at metagenome level, largely eliminating prior laboratory culturing steps. (chalmers.se)
  • Metagenomics has thereby been established as a new interdisciplinary field and methodology, harmonizing the accumulated knowledge in microbial ecology and genetics with the high-throughput environmental DNA sequence data through the means of bioinformatics analysis resources. (chalmers.se)
  • analysis of living b
  • The improved rejection of out-of-focus haze and the increased resolution of SCLM made it preferable to conventional phase microscopy for the analysis of living biofilms. (montana.edu)
  • consortium
  • The Biofilm mode of growth provides such significant advantages to the members of the consortium that most organisms in important habitats are found in biofilms. (unt.edu)
  • The biofilm growth state incorporates a second important factor, the heterogeneity in the distribution in time and space of the component members of the biofilm consortium. (unt.edu)
  • Unlike typical soluble substrates that have been used as electron donors in MFC studies, cellulose is unique because it requires a microbial consortium that can metabolize both an insoluble electron donor (cellulose) and electron acceptor (electrode). (iwaponline.com)
  • A sulfurbased microbial consortium together with several algal groups were hypothesized to partake in the detoxification of triclosan. (chalmers.se)
  • form biofilms
  • The quantification of biofilm relays currently on a number of different approaches and techniques, often leading to different evaluations of the ability to form biofilms of the studied microbial strains. (mdpi.com)
  • This study was, therefore, planned with the aim of exploring the potential of the bacterial and fungal agents causing orbital cellulitis and CRS to form biofilms, and secondly to find out, if such biofilms could circumvent the effect of antibacterial and antifungal agents by restricting their entry through the sessile biofilm architecture in an in vitro model, designed in our laboratory. (springer.com)
  • antimicrobial
  • The purpose of the present study was to look for the adherent property and biofilm producing ability of the clinical isolates in chronic rhinosinusitis and orbital cellulitis, and to look for the effects of antimicrobial agents on these biofilms by colorimetric assay and ultrastructural analysis. (springer.com)
  • quantified and antimicrobial drug perfusion through the biofilm model was evaluated by the earlier devised procedure. (springer.com)
  • Antimicrobial drug perfusion through the biofilm model was poor. (springer.com)
  • Antimicrobial treatment was totally ineffective against strong biofilm producers, whose electron microscopic picture was quite similar to that observed for biofilm producers without any antimicrobial pre-treatment. (springer.com)
  • Major efforts directed towards preventing and eradicating the biofilm problem face difficulties because biofilms protect themselves very effectively by producing a polysaccharide coating, reducing biofilm sensitivity to antimicrobial agents. (mdpi.com)
  • Adverse effects of anthropogenic impact on the environment have become conspicuous in the past century and among others include the gradual increase in the global CO2 levels, the contamination of air, soil and water by toxic chemicals, and the emergence of antimicrobial resistance among pathogenic microbial species. (chalmers.se)
  • Paper III also constituted the pilot study for Paper IV, where an experimental design was set up to investigate the toxic effects of the broad spectrum antimicrobial agent, triclosan, on the marine biofilms. (chalmers.se)
  • populations
  • To address this challenge, our team of microbiologists, mathematicians and computer scientists has been developing software applications that can accurately process digital images of actively growing microbial populations and communities in natural and managed habitats, and extract an abundance of ecologically relevant data from them. (mdpi.com)
  • antibiotic
  • Interestingly, antibiotics can actually enter a biofilm in many instances, but the EPS actively shields its inhabitants from such compounds, promoting antibiotic resistance. (medicalnewstoday.com)
  • In this work, I utilized unique methods for studying factors contributing to cooperative antibiotic detoxification in a polymicrobial biofilm model. (gsu.edu)
  • Costerton
  • On the other hand, CSLM, which allows the visualization of fully hydrated samples, has revealed the elaborate three-dimensional structure of biofilms (Costerton 1995). (studentshare.net)
  • diversity
  • The variety of microniches provided by the biofilm also promotes a huge diversity of microbial life and metabolic potential. (caister.com)
  • Amplification of the 16S rRNA gene from selected NCs provided a substantially larger number of operational taxonomic units per NC than did plate counts (26 to 43 versus 1 to 4 operational taxonomic units/NC, respectively), suggesting that culture-dependent methods may substantially underestimate microbial diversity on NCs. (asm.org)
  • NCs provide a portal of entry for a wide diversity of opportunistic pathogens to colonize the catheter lumen, forming a biofilm and increasing the potential for CAI, highlighting the importance of catheter maintenance practices to reduce microbial contamination. (asm.org)
  • organisms
  • The metagenomic analysis of the marine biofilms revealed that Proteobacteria, Bacteroidetes and Cyanobacteria are the most abundant organisms in these biofilm communities. (chalmers.se)
  • ecological
  • In this PhD thesis, bioinformatics software development and microbial ecological data analysis projects are integrated within the field of ecotoxicology. (chalmers.se)
  • 2000
  • de Beer, D. and P. Stoodley, "Microbial biofilms," In: The Prokaryotes: An Evolving Electronic Resource for the Microbiological Community, 3rd edition (release 3.4), New York, Springer-Verlag, 2000, [www.prokaryotes.com], Accessed 01/04/01 ISBN: 0-387-14254-1. (montana.edu)
  • investigate
  • This project will provide the foundation for developing capabilities to investigate microbial biofilms, which will have applications in environmental, energy, and health sciences. (pnnl.gov)
  • study
  • Beech IB (2004) Corrosion of technical materials in the presence of biofilms-current understanding and state-of-the art methods of study. (springer.com)
  • Objective: This study used a novel model of multispecies bacterial biofilms to stimulate oral epithelial cells and profile select cytokines and chemokines that contribute to the local inflammatory environment in the periodontium. (umich.edu)
  • This classification is putatively amenable of further study by comparing the biofilm type and the medical behavior of the strains. (mdpi.com)
  • Electronmicroscopic study of the biofilm was performed by the recommended technique. (springer.com)
  • Literature study is available on the influence of hydrodynamic conditions on the physical structure of the biofilms i.e. high shear forces result in thinner, denser, stronger and filamentous biofilms while low shear forces result in monolayer of biofilm cells with mound and circular shape. (unisa.ac.za)
  • One of the emerging application areas that require a comprehensive microbial investigation is the study of the effects of toxic chemicals on biota in the environment, namely ecotoxicology. (chalmers.se)
  • Energy-dispersive X-ray spectroscopy suggested that these grains are compositionally similar to the mineral strengite (FePO 4∙ 2H 2 O). This study clearly indicates that the Arctic supports a thriving microbial community that influences the biogeochemical cycling of PO 4 in an environment of low nutrient availability. (geoscienceworld.org)
  • planktonic
  • The microbial cells growing in a biofilm are physiologically distinct from planktonic cells of the same organism, which, by contrast, are single-cells that may float or swim in a liquid medium. (scribd.com)
  • Inhibition
  • Inhibition of the growth of a formed biofilm was observed only after treatment with Miramistin in the high doses (25-50 μg/ml). (springer.com)
  • succession
  • Callbeck CM, Dong X, Chatterjee I, Agrawal A, Caffrey SM, Sensen CW, Voordouw G (2011) Microbial community succession in a bioreactor modeling a souring low-temperature oil reservoir subjected to nitrate injection. (springer.com)
  • phenotypic
  • When a cell switches to the biofilm mode of growth, it undergoes a phenotypic shift in behavior in which large suites of genes are differentially regulated. (scribd.com)
  • cellular
  • These optical sections were then analyzed by image-processing techniques to assess the distribution of cellular and noncellular areas within the biofilm matrices. (montana.edu)
  • Cellular changes were buffered by both the social benefits and the protective physico-chemical milieu of the interior of biofilms. (biomedcentral.com)
  • Corrosion
  • In the laboratory, the inventory of potentially corrosive microorganisms is increasing and microbial biochemical and bioelectrical processes are now recognized to be involved in corrosion. (springer.com)
  • Duan J, Wu S, Zhang X, Huang G, Du M, Hou B (2008) Corrosion of carbon steel influenced by anaerobic biofilm in natural seawater. (springer.com)
  • In addition, biofilms can affect the quality and yield of crops and cause biofouling and microbially-induced corrosion. (caister.com)
  • development
  • However in contrast, there are conflicting reports, demonstrating that QS appears to play a minimal role in the development of biofilms. (caister.com)
  • We also discuss QS and cooperation in the context of social evolution and how this may impact on the development and maintenance of microbial biofilms. (caister.com)
  • CSLM has been used very effectively to monitor biofilm development in flow cells. (studentshare.net)
  • growth
  • Treatment of formed biofilms with the agent in doses of 6.0 and 0.6 mg/ml was associated with pronounced stimulation of its growth in S. pyogenes , S. aureus , and L. acidophilus . (springer.com)
  • It is assumed that other flow phenomenon present may have a significant effect on biofilm growth and structure and may distort conclusions when compared alongside the simpler flow structure of the two methods considered. (unisa.ac.za)