Microarray expression analysis. Medical search. Frequent questions

RNA extracted from mock-infected and WNV-infected cells was assessed for differential expression of genes using Affymetrix microarray. (molvis.org)
Quantitative real-time PCR analysis of 23 genes was used to validate the microarray results. (molvis.org)
Functional annotation clustering of the microarray data showed that gene clusters involved in immune and antiviral responses ranked highly, involving genes such as chemokine (C-C motif) ligand 2 ( CCL2 ), chemokine (C-C motif) ligand 5 ( CCL5 ), chemokine (C-X-C motif) ligand 10 ( CXCL10 ), and toll like receptor 3 ( TLR3 ). (molvis.org)
Low-level WNV infection of hRPE cells induced expression of genes that are typically associated with the host cell response to virus infection. (molvis.org)
DAVID was used to perform the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis for the different expression genes (DEGs). (medscimonit.com)
Ten DEGs, including 7 hub genes from PPI analysis, were confirmed by real-time PCR. (medscimonit.com)
Then, the DEGs were analysed to identify biological pathways of dental pulp inflammation with Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis and Gene Set Enrichment Analysis (GSEA). (researchsquare.com)
A consensus list of 643 genes (784 probe sets) with greater than 2-fold difference between intratumoral periphery and core samples was obtained using Microarray Suite 5.0, model-based expression indexes, and robust multiarray analysis algorithms. (port.ac.uk)
Microarray analysis reveals that inhibition of Notch signalling causes downregulation of stem cell-associated genes in parallel with decreased proliferation and increased numbers of ciliated cells and that organoids also respond to oestradiol and progesterone treatment in a physiological manner. (nature.com)
Pathway analysis of the significant genes showed that the genes were involved in known metastasis-associated pathways, such as integrin signaling, calcium signaling, and VEGF signaling. (nih.gov)
By screening specific genes related to the muscle growth of swine using cDNA microarray technology, a total of 5 novel genes (GF (growth factor) I, II, III, IV and V) were identified. (animbiosci.org)
Expression patterns of these genes in other organs, namely muscle and propagation and fat tissues, were investigated by extracting RNA from the tissues. (animbiosci.org)
The wide use of high-throughput DNA microarray technology provide an increasingly detailed view of human transcriptome from hundreds to thousands of genes. (biomedcentral.com)
Although biomedical researchers typically design microarray experiments to explore specific biological contexts, the relationships between genes are hard to identified because they are complex and noisy high-dimensional data and are often hindered by low statistical power. (biomedcentral.com)
In our study, two unsupervised knowledge-based matrix factorization methods, independent component analysis (ICA) and nonnegative matrix factorization (NMF) are integrated to identify significant genes and related pathways in microarray gene expression dataset of Alzheimer's disease. (biomedcentral.com)
The simulation results shows that both of the methods can clearly classify severe AD samples from control samples, and the biological analysis of the identified significant genes and their related pathways demonstrated that these genes play a prominent role in AD and relate the activation patterns to AD phenotypes. (biomedcentral.com)
By combining the significant genes identified by both ICA and NMF, the biological analysis shows great efficient for elucidating the molecular taxonomy of Alzheimer's disease and enable better experimental design to further identify potential pathways and therapeutic targets of AD. (biomedcentral.com)
Scientists use DNA microarrays to measure the expression levels of large numbers of genes simultaneously or to genotype multiple regions of a genome. (wikipedia.org)
In this study, we performed a new bioinformatics analysis on microarray-based gene expression data obtained from the public domain to explore genes that were differentially induced by drugs between the group of capreomycin and PA-824 and the group of mainly the first-line TB drugs. (hindawi.com)
The microarray system permitted screening of large numbers of genes in biological or pathological processes. (kyobobook.co.kr)
The data suggest that the OGD has general suppressive effect on the gene expression with the exception of some genes which are related with ischemic cell death directly or indirectly. (kyobobook.co.kr)
Whole genome cDNA microarrays as well as oligonucleotide microarrays including leukocyte-relevant genes will be used to characterize potential hgh-specific genes by comparing gene expression profiles of treated and untreated cells. (wada-ama.org)
After confirming SSH-genes to be differentially expressed in a series of microarray experiments, oligonucleotide probes will be designed for both SSH-genes and the candidate genes identified along gene expression profiling using whole genome/leukocyte relevant arrays. (wada-ama.org)
To establish a selective "hgh-chip" the newly designed oligonucleotide probes for the hghcandidate genes will be printed on a microarray. (wada-ama.org)
It regulates Sp7 protein expression and induces expression of major bone matrix protein genes, such as Col1a1, Spp1, Ibsp, Bglap2, and Fn1 ( 7 , 8 ). (frontiersin.org)
Gene expression was studied by cDNA microarrray analyses using 4608 unique clones from liver-derived expressed sequence tag (EST) libraries fortified with clones of known liver genes representing ∼4000 genes. (houstonmethodist.org)
In conclusion, our results strongly suggest that sustained induction of P4501A1 by MC is accompanied by persistent expression of other genes belonging to the Ah gene battery, as well as certain other genes involved in toxic responses. (houstonmethodist.org)
Gene expression array data were used as a training set to screen for single genes with prognostic impact. (scilifelab.se)
The prognostic impact of one of these genes, the cell adhesion molecule 1 (CADM1), was confirmed by use of immunohistochemistry on tissue microarrays from 2 independent NSCLC cohorts, altogether including 617 NSCLC samples. (scilifelab.se)
Using a novel NSCLC cohort together with a meta-analysis validation approach, we have identified a set of single genes with independent prognostic impact. (scilifelab.se)
DNA microarrays make it possible to analyze the mRNA expression of thousands of genes simultaneously. (karger.com)
The resulting comprehensive gene expression surveys lead to the identification of new genes and pathways with importance in cancer development and progression, or as targets for new therapies. (karger.com)
The validation and prioritization of genes emerging from genome screening analyses in large series of clinical tumors has become a new bottleneck in research. (karger.com)
Therefore, we have recently developed the tissue microarray (TMA) technology to efficiently test the clinical relevance of candidate genes. (karger.com)
DNA microarrays and TMAs provide a powerful approach to identify large numbers of new candidate genes, and rapidly validate their clinical impact in large series of human tumors. (karger.com)
The sequence analysis of the 32-35,000 predicted genes indicates the there may be as many as 1,000 kinase or kinase-like genes. (ddw-online.com)
In today's research laboratory, two common ways that gene expression is assessed is by using quantitative polymerase chain reaction techniques (QTPCR) for monitoring expression of smaller numbers of genes, and by employing DNA microarrays for the highly parallel monitoring of thousands of genes (or the whole transcriptome). (ddw-online.com)
Analysis of gene expression can include measuring expression levels of a small set of genes to whole genome expression monitoring. (ddw-online.com)
A potentially powerful way to gain insight in the complex pathogenesis of rheumatoid arthritis (RA) and to classify arthritides has arisen from cDNA microarray technology, which provides the opportunity to determine differences in gene expression of a large portion of the genome in search of genes that are differently expressed between clinically diagnosed arthritides. (biomedcentral.com)
The results revealed 1066 genes with a twofold difference in expression in at least 4 samples, relative to the median Cy-5 to Cy-3 ratio. (biomedcentral.com)
In this study, we performed a bioinformatics analysis of the entire maize genome and identified 40 CDPK genes. (biomedcentral.com)
Most maize CDPK genes exhibited different expression levels in different tissues and developmental stages. (biomedcentral.com)
Expression analyses suggested that maize CDPK genes are important components of maize development and multiple transduction pathways. (biomedcentral.com)
Here, we present a genome-wide analysis of the CDPK gene family in maize for the first time, and this genomic analysis of maize CDPK genes provides the first step towards a functional study of this gene family in maize. (biomedcentral.com)
The gene expression of Ppargc1a varied during scleral growth from week 1 to 2, week 2 to 3, week 3 to 6, and week 6 to 8 and was found to interact with a different set of genes at different scleral growth stages. (molvis.org)
DNA microarray expression profiling indicated that dietary administration of NDGA upregulated the expression of certain genes involved in fatty acid oxidation and their transcription regulator, PPARα, decreased the expression of a number of lipogenic genes and relevant transcription factors, and differentially impacted the genes of fatty acid transporters, acetyl CoA synthetases, elongases, fatty acid desaturases and lipid clearance proteins in liver, skeletal muscle and adipose tissues. (biomedcentral.com)
These findings suggest that NDGA ameliorates hypertriglyceridemia and steatosis primarily by inhibiting lipogenesis and enhancing fatty acid catabolism in three major insulin responsive tissues by altering the expression of key enzyme genes and transcription factors involved in de novo lipogenesis and fatty acid oxidation. (biomedcentral.com)
Statistical methods for identifying differentially expressed genes in replicated cDNA microarray experiments. (statsci.org)
Cancer genomics analysis revealed that 36 of the most differentially expressed mRNAs were involved in a pancreatic cancer network and were associated with many critical mutated genes such as TP53, KRAS, SMAD4, and CDKN2A. (jcancer.org)
Data from these define genetic and non-genetic variation, define a measure of heritability for expression of individual genes, and provide the basis for a new method of data reduction for genetical genomics. (biomedcentral.com)
The expression of transthyretin, a protein shown to sequester Aβ and prevent amyloid fibril formation in vitro , and several genes in the insulin-signaling pathway, e.g., insulin-like growth factor-2, were increased selectively in the hippocampus of APP Sw mice. (jneurosci.org)
The introduction of complementary DNA (cDNA) microarray technology in 1995 has helped to facilitate the identification and classification of DNA sequence information and the assignment of functions to these new genes by allowing investigators to analyze expression of thousands of genes simultaneously in a single experiment. (medscape.com)
Microarrays are a significant advance because of their small size and are therefore useful when one wants to survey a large number of genes quickly or when the study sample is small. (medscape.com)
Because a microarray can be used to examine the expression of hundreds or thousands of genes at once, it promises to revolutionize the way gene expression is examined. (medscape.com)
DNA microarrays are simply platforms that consist of small solid supports onto which the sequences from thousands of different genes are attached at fixed locations. (medscape.com)
We also identified an epigenetic signature common to all cases, with correlation to gene expression in 65% of these genes, suggesting that a core set of epigenetically deregulated genes is central to the initiation or maintenance of lymphoid transformation. (lu.se)
Finally, we identified aberrant methylation in multiple genes also targeted by recurring DNA copy number alterations in ALL, suggesting that these genes are inactivated far more frequently than suggested by structural genomic analyses alone. (lu.se)
Oncotype DX is a quantitative RT-PCR assay that measures the expression of 17 genes (12 cancer-specific and five housekeeping genes). (medscape.com)
The score is based on the microarray-based expression of 22 genes involved in androgen receptor signaling, cell proliferation, differentiation, motility, and immune modulation. (medscape.com)
By monitoring microbial gene expression, one can predict the functions of uncharacterized genes, probe the physiologic adaptations made under various environmental conditions, identify virulence-associated genes, and test the effects of drugs. (medscape.com)

The ankylosed calluses were harvested at days 14 and 28 postoperatively and analyzed by Affymetrix OviGene-1_0-ST microarrays. (medscimonit.com)
These pipelines consist of a set of tools for GFF file processing of NimbleGen chromatin immunoprecipitation on microarray (ChIP-chip) datasets and more comprehensive workflows for Affymetrix gene expression microarray bioinformatics and basic primer design for PCR experiments, which are often used to validate microarray results. (biomedcentral.com)
It is shown how to fully exploit the power of genetics through optimal experimental design and analysis for two major microarray technologies, cDNA two-colour arrays and Affymetrix short oligonucleotide arrays. (open.ac.uk)
MA-plots for single channel microarray platforms such as Affymetrix are computed from the means and differences of log-expression values from two microarrays. (statsci.org)
Data reduction is an issue because Affymetrix GeneChip oligonucleotide microarrays assay each target mRNA with a set of 11 to 16 pairs of 25-nucleotide DNA probes. (biomedcentral.com)
The Affymetrix software Microarray Suite 4.0 and 5.0 (MAS 4 and MAS 5) estimate expression from the average difference of PM and MM fluorescence. (biomedcentral.com)
Oligonucleotide microarrays are manufactured by Affymetrix (Santa Clara, Calif) using photolithographic techniques. (medscape.com)

Unsupervised matrix factorization methods provide efficient tools to analyze high-throughput microarray dataset. (biomedcentral.com)
Modern signal processing and machine learning techniques provide new and efficient analysis tools to the high-throughput microarray gene expression dataset. (biomedcentral.com)
Several independent high-throughput methods for differential gene expression (including SAGE and differential display) may enable function annotation of sequenced genomes [ 2 ] . (medscape.com)
DNA microarray hybridization analysis stands out for its simplicity, comprehensiveness, data consistency, and high throughput. (medscape.com)
High-throughput RNA sequencing (RNA-seq) has revolutionized our understanding of gene expression. (cdc.gov)

Microarray technologies have come into prominence for the assessment of molecular diagnostic profiles in cancer tissue biopsies. (port.ac.uk)
To obtain insight into the renewal activity of the human fallopian tube mucosa, we carried out immunofluorescence analysis of healthy samples from gynaecological tissue specimens. (nature.com)
Biomarker discovery in non-small cell lung cancer: integrating gene expression profiling, meta-analysis, and tissue microarray validation. (scilifelab.se)
One class revealed abundant expression of gene clusters indicative of the presence and activation of the adaptive immune response, and the other group resembled the expression pattern of the OA tissues, which is characterized by a low inflammatory gene expression signature and increased tissue remodeling. (biomedcentral.com)
A Tissue Microarray Study of Penile Squamous Cell Carcinomas. (gov.py)
Herein we assess p53 immunohistochemical expression on tissue samples of penile SCC using freely-available, open-source software packages for digital image analysis. (gov.py)
Fas, FADD, caspase 8, CDC25B, fascin, CK14, CK4, annexin I, laminin-5γ2 and SPARC were analyzed using immunohistochemistry on tissue microarray containing 205 ESCC and 173 adjacent precursor lesions as well as corresponding normal mucosa. (biomedcentral.com)
To confirm the immunohistochemical results, three proteins, fascin, CK14 and laminin-5γ2, which were overexpressed in ESCC on tissue microarray, were detected in 12 ESCC cell lines by Western blot assay. (biomedcentral.com)
The method of tissue microarray (TMA), which could confirm the results of cDNA microarray on protein level, has been popularly used for gene expression profiling in many types of cancer including ESCC, but rarely in the consecutive stages of carcinogenesis, especially in the precursor lesions. (biomedcentral.com)
Microarrays may be used to assay gene expression within a single sample or to compare gene expression in 2 different cell types or tissue samples, such as in healthy versus diseased tissue. (medscape.com)
Decipher is the most broadly validated tissue-based gene expression classifier. (medscape.com)

The core principle behind microarrays is hybridization between two DNA strands, the property of complementary nucleic acid sequences to specifically pair with each other by forming hydrogen bonds between complementary nucleotide base pairs. (wikipedia.org)
DNA microarrays can be used to detect DNA (as in comparative genomic hybridization), or detect RNA (most commonly as cDNA after reverse transcription) that may or may not be translated into proteins. (wikipedia.org)
They can be used for large-scale, massively parallel in situ analysis of genetic alterations on a DNA, RNA and protein level using in situ hybridization or immunohistochemistry on hundreds of tumor specimens at a time. (karger.com)
Transcribed RNA levels are often measured by hybridization to microarrays of oligonucleotide probes, in which each transcript is represented by multiple probes. (biomedcentral.com)
Both technologies use hybridization of labeled nucleic acid transcripts to measure the gene expression. (medscape.com)

According to the facts that different unsupervised approaches explore correlations in the high-dimensional data space and identify relevant subspace base on different hypotheses, integrating these methods to explore the underlying biological information from microarray dataset is an efficient approach. (biomedcentral.com)
An MC3T3-E1-based microarray dataset (accessioned as GSE46400) downloaded from the Gene Expression Omnibus included MC3T3-E1 cells with or without 14-day osteoblast differentiation osteoblast induction. (frontiersin.org)

DNA microarrays take advantage of the major feature of the DNA double helix, the sequence complementarity of the two-paired strands, by using DNA capture probes which are the complement of the expressed target sequence (mRNA, cRNA or cDNA made from the mRNA). (ddw-online.com)
Chen J, Wang S, Jia S, Ding G, Jiang G, Cao L. Integrated Analysis of Long Non-Coding RNA and mRNA Expression Profile in Pancreatic Cancer Derived Exosomes Treated Dendritic Cells by Microarray Analysis. (jcancer.org)
We used integrated lncRNA and mRNA microarrays to determine the expression profiles of PEX-treated DCs and normal DCs derived from five healthy donors. (jcancer.org)
Because the locations of the probes are known, the intensity and pattern of the labeled mRNA can be used to measure the expression of the targeted gene. (medscape.com)

These results suggest that microarray analysis of gene expression may be useful for screening novel molecular mediators of astrocyte response to ischemic injury and making profound understanding of the cellular mechanisms as a whole. (kyobobook.co.kr)
Finally, we suggest that microarray data processing task workflows may provide a basis for future example-based comparison of different workflow systems. (biomedcentral.com)

3. Translational research - to identify predictive biomarkers for treatment decision - by immunohistochemistry, gene expression or sequencing. (lu.se)

By integrating different datasets from the Gene Expression Omnibus (GEO) database, we analysed a merged expression matrix of pulpitis, aiming to identify biological pathways and diagnostic biomarkers of pulpitis. (researchsquare.com)
GO analysis revealed that the DEGs were enriched in biological processes related to inflammation, and the enriched pathways in the KEGG pathway analysis were cytokine-cytokine receptor interaction, chemokine signalling pathway and NF-κB signalling pathway. (researchsquare.com)
Analysis of functional categories and signaling and biochemical pathways associated with the response of HeLa cells to treatment with RuT7 showed that it leads the cells through the intrinsic (mitochondrial) apoptotic pathway, via indirect DNA damage due to the action of reactive oxygen species, and through direct DNA binding of RuT7. (ac.rs)
Gene Ontology (GO), KEGG, and cancer genomics analyses were performed to identify significant functions, pathways, and the associations of differentially expressed mRNAs. (jcancer.org)
COVID-19: disease pathways and gene expression changes predict methylprednisolone can improve outcome in severe cases. (cdc.gov)

In our work, we performed FastICA and non-smooth NMF methods on DNA microarray gene expression data of Alzheimer's disease respectively. (biomedcentral.com)
Biomedical researchers will find this book invaluable for learning the cutting-edge methods for analyzing gene expression microarray data. (firebaseapp.com)
We have tested heritability-weighted averages to define expression of a transcript and found that these allow detection of more QTLs than previously described methods. (biomedcentral.com)
Several alternative methods have been proposed to combine multiple probe-specific values into a single expression estimate. (biomedcentral.com)
A secondary goal that should be sought by a bioinformatics facility is to make algorithms and analysis methods accessible for non-computational biologists. (lu.se)
Methods for gene identification and analysis of gene structure: cloning, PCR, restriction mapping, in situ hybridisation, DNA sequencing. (lu.se)
Methods for analysis of gene expression: microarray, hybridisation, promoter analyses. (lu.se)

With the widespread use of the DNA microarray technology in the study of biology and medicine, large amount of gene expression data have been easily accumulated. (biomedcentral.com)
This book focuses on the development and application of the latest advanced data mining, machine learning, and visualization techniques for the identification of interesting, significant, and novel patterns in gene expression microarray data. (firebaseapp.com)
Results obtained from gene expression profiling utilising whole genome arrays were in accordance with published data. (wada-ama.org)
Microarray data analysis has been the subject of extensive and ongoing pipeline development due to its complexity, the availability of several options at each analysis step, and the development of new analysis demands, including integration with new data sources. (biomedcentral.com)
We provide a set of tools and complete workflows for microarray data analysis in the Kepler environment, which has the advantages of offering graphical, clear display of conceptual steps and parameters and the ability to easily integrate other resources such as remote data and web services. (biomedcentral.com)
There have been dimensional increases in 'omics datasets, including introduction of new types of data, increases in the size of individual datasets, increases in the varieties of experimental platforms within a given data type (e.g. more varieties of gene expression microarrays), and very large increases in the total number of datasets being generated. (biomedcentral.com)
In total, this has led to a highly dynamic and rapidly changing analysis environment, with increasingly complicated and non-standard data analysis needs. (biomedcentral.com)
In this paper it is argued that the design and analysis of such a study is not a matter of simply applying the existing and more-or-less standard computational tools for microarrays to a new type of experimental data. (open.ac.uk)
Bowtell DD: Options available - from start to finish - for obtaining expression data by microarray. (karger.com)
Quackenbush J. Computational analysis of microarray data . (udel.edu)
An MA-plot of microarray data is a plot of log-ratio of two expression intensities versus the mean log-expression of the two. (statsci.org)
Transformations for cDNA Microarray Data. (statsci.org)
Data analysis from preprocessing to clustering and basics of inferring gene regulation. (helsinki.fi)
BASE was initially developed to manage array-based data but is now extended to support storage and analysis of sequencing data. (lu.se)
Analyses include generation of gene expression and sequencing data with the ultimate goal to build an infrastructure for future real-time clinical implementation. (lu.se)
Analysis and execution of standard analysis pipelines for sequencing data will be performed through BASE. (lu.se)
This particular BASE installation was initially set up in 2003 and to date manage array data from more than 13 000 hybridisation covering a variety of technical platforms such as cDNA, oligo, and BeadChip expression arrays, as well as BAC and oligo aCGH arrays. (lu.se)
The data produced from a microarray experiment typically constitute a long list of measurements of spot intensities and intensity ratios, generated either by a pair-wise comparison of 2 samples or by a comparison of several samples with a common control. (medscape.com)
Because of the complexity of the data sets generated by microarray experiments, the use of data-analysis software is essential. (medscape.com)
Several commercial and public data-analysis tools have been developed for this purpose. (medscape.com)
To analyze microarray datasets (34760 variables) and obtain functional implications of differential expression patterns, we used Linear Models for Microarray Data (limma) followed by Gene Set Enrichment Analysis (GSEA) using ClusterProfiler. (lu.se)
They also analyse data from microarray platforms such as gene expression arrays. (lu.se)
Other analyses not mentioned above can also be performed on your data, time-permitting. (lu.se)
DNA microarrays exploit primary sequence data to measure transcript levels and detect sequence polymorphisms, for every gene, simultaneously. (medscape.com)
WeNNI is a method for imputing unreliable expression data values. (lu.se)

Computational nodes are connected to the main computer and used to run configured analysis plugins in a seamless integrated fashion. (lu.se)

This is the peer-reviewed version of the article: Jovanovic KK, Tanic M, Ivanovic I, Gligorijević N, Dojčinović B, Radulovic S. Cell cycle, apoptosis, cellular uptake and whole-transcriptome microarray gene expression analysis of HeLa cells treated with a ruthenium(II)-arene complex with an isoquinoline-3-carboxylic acid ligand. (ac.rs)
To gain further insight in the molecular mechanism of action of RuT7 on HeLa cells, a whole-transcriptome microarray gene expression analysis was performed. (ac.rs)

High-resolution, whole-genome microarray analysis is becoming the standard for hematological cancer sample profiling and and may one day replace traditional cytogenetic techniques. (thermofisher.com)

DEGs in GO analysis were enriched in biological processes about inflammation and in KEGG pathway analysis were cytokine-cytokine receptor interaction, chemokine signaling pathway and NF-κB signaling pathway. (researchsquare.com)
A possible pathway that contributes to the postnatal development of the sclera was investigated using Ingenuity Pathway Analysis software. (molvis.org)
GO and pathway analysis revealed differentially expressed mRNAs involved in many critical biological processes and molecular functions. (jcancer.org)
Mice were sacrificed at 0, 28, and 84 d post-exposure and pulmonary global gene expression results were analyzed by QIAGEN's Ingenuity Pathway Analysis. (cdc.gov)

The "hgh-chip" will then be thoroughly evaluated by performing numerous gene expression experiments on hgh-treated and untreated leukocyte subsets, cultured PBMCs as well as PBMCs obtained from hgh-patients. (wada-ama.org)
The persistent induction of P4501A1 gene expression was confirmed by real time reverse transcriptase polymerase chain reaction (RT-PCR) experiments. (houstonmethodist.org)

Therefore, the gene expression patterns in the in vitro model of astrocytes following exposure to oxygen-glucose deprivation (OGD) were evaluated by using the microarray analysis. (kyobobook.co.kr)
Female Sprague-Dawley rats were treated with MC (100 μmol/kg) ip once daily for 4 days, and gene expression patterns were investigated using total liver RNA isolated from animals at 1, 15, and 28 days after MC withdrawal. (houstonmethodist.org)
Analyzing the protein expression patterns of Fas, FADD, caspase 8, CDC25B, fascin, CK14, CK4, annexin I, laminin-5γ2 and SPARC would be valuable to develop rational strategies for early detection of lesions at risk in advance as well as for prevention and treatment of ESCC. (biomedcentral.com)
Spatial patterns of gene expression in the olfactory bulb. (statsci.org)

We analyzed 137 B-lineage and 30 T-lineage childhood ALL cases using microarray analysis of DNA copy number alterations and gene expression, and genome-wide cytosine methylation profiling using the HpaII tiny fragment enrichment by ligation-mediated PCR (HELP) assay. (lu.se)

Expression profiling of tens to hundreds of individuals in a genetic population can reveal the consequences of genetic variation. (open.ac.uk)
Two of the most common uses of the DNA microarrays are genetic analysis and the analysis of gene expression. (ddw-online.com)
Genetic analysis includes procedures for genotyping, SNP (single nucleotide polymorphism) detection, strain identification and various other procedures. (ddw-online.com)
Previous studies of DNA methylation suggest epigenetic alterations may also be important, but an integrated genome-wide analysis of genetic and epigenetic alterations in ALL has not been performed. (lu.se)
We found that the different genetic subtypes of ALL are characterized by distinct DNA methylation signatures that exhibit significant correlation with gene expression profiles. (lu.se)

Microarrays can be manufactured in different ways, depending on the number of probes under examination, costs, customization requirements, and the type of scientific question being asked. (wikipedia.org)
In spotted microarrays, the probes are oligonucleotides, cDNA or small fragments of PCR products that correspond to mRNAs. (wikipedia.org)
Microarray analysis is a method that uses microchips containing anchored arrays of short DNA elements (known as probes) for the large-scale interrogation of gene expression. (nature.com)
The use of recombinant inbred lines allows an estimate of the heritability of expression measured by individual probes. (biomedcentral.com)

Low CADM1 protein expression was significantly associated with shorter survival, with particular influence in the adenocarcinoma patient subgroup. (scilifelab.se)

Mutations in TCF8 cause posterior polymorphous corneal dystrophy and ectopic expression of COL4A3 by corneal endothelial cells. (nih.gov)

We developed a series of fully functional bioinformatics pipelines addressing common tasks in microarray processing in the Kepler workflow environment. (biomedcentral.com)
In this study, we are interested in investigating the expression of a variety of functional classes of proteins in ESCC and its precursor lesions and characterizing the correlation of these proteins with ESCC malignant progression. (biomedcentral.com)

To better understand the effect of sampling bias, we paired image-guided stereotactic biopsy and microarray technology to study regional intratumoral differences in tumor periphery and core regions of untreated glioblastoma. (port.ac.uk)
Microarray technology enables the flexible throughput analysis of genotyping, chromosome copy number variations and gene expression. (thermofisher.com)
In this video, he discusses the use of microarray technology and how it facilitates a deeper understanding of oncogenesis. (thermofisher.com)
The present project aims to introduce microarray technology as a new analytical tool into the field of doping control analysis to gain insight in specific effects of recombinant human growth hormone (hgh) on blood cells (leukocytes). (wada-ama.org)
Microarray technology will be used to search for changes in leukocyte gene expression which are directly related to the application of human growth hormone (hgh). (wada-ama.org)
Knowledge generated along this research project and the introduction of microarray technology into doping analysis should open novel approaches and strategies for future detection of doping substances. (wada-ama.org)
The DNA microarray technology within SCIBLU was initially established in 2000 as a cancer research resource at the department of Oncology and in conjunction with this the development of BASE was initiated. (lu.se)

Molecular mechanisms for regulation of gene expression at different levels: remodeling of chromatin, initiation of transcription, nuclear transport and signalling, and RNA interference. (lu.se)

The process of measuring gene expression via cDNA is called expression analysis or expression profiling. (wikipedia.org)
Global gene expression profiling has been widely used in lung cancer research to identify clinically relevant molecular subtypes as well as to predict prognosis and therapy response. (scilifelab.se)
It is clear that gene expression profiling systems have a role to play in many facets of drug discovery and development, but several questions remain. (ddw-online.com)
We take a look at the use of DNA microarray for gene expression or expression profiling and how DNA chips are used for drug discovery and development as a whole. (ddw-online.com)
This report will focus in using DNA microarrays for the analysis of gene expression or expression profiling, and how DNA chips are used for drug discovery and development. (ddw-online.com)
Host profiling might also identify gene expression signatures unique for each pathogen, thus providing a novel tool for diagnosis, prognosis, and clinical management of infectious disease. (medscape.com)
Global profiling of gene expression is one attractive approach to assessing function. (medscape.com)

BACHD mice with ubiquitous expression of full-length mutant HTT (mHTT) and wild-type mice with targeted hypothalamic overexpression of either wild-type HTT (wtHTT) or mHTT fragments. (lu.se)

Results were validated using quantitative polymerase chain reaction and Western blotting analyses. (port.ac.uk)
RT-qPCR results confirmed the significant expression of pyruvate dehydrogenase kinase 3 (PDK3), cyclin-dependent kinase 8 (CDK8), activating transcription factor 3 (ATF3), fibulin-7 (Fbln-7), hyaluronan synthase 1 (HAS1), interleukin 4 receptor (IL-4R) and apolipoprotein C1 (ApoC1). (bvsalud.org)
We also compared the results of digital analysis with standard visual estimation. (gov.py)

To our knowledge, this is the first study to detect the differential expression of lncRNAs and mRNAs associated with PEX-treated DCs. (jcancer.org)

Gene expression of eye diseases should be studied as early as postnatal weeks 1-2 to ensure that any changes in gene expression pattern during disease development are detected. (molvis.org)

Learn more about molecular and biomarker analysis in PCa. (medscape.com)

The original nucleic acid arrays were macro arrays approximately 9 cm × 12 cm and the first computerized image based analysis was published in 1981. (wikipedia.org)
An example of its application is in SNPs arrays for polymorphisms in cardiovascular diseases, cancer, pathogens and GWAS analysis. (wikipedia.org)
Microarrays can be fabricated using a variety of technologies, including printing with fine-pointed pins onto glass slides, photolithography using pre-made masks, photolithography using dynamic micromirror devices, ink-jet printing, or electrochemistry on microelectrode arrays. (wikipedia.org)

The differences in the gene expression profiles reflect important aspects of biological variation within the clinically diagnosed arthritides that may help to understand the molecular pathology of and (sub-)classify rheumatic diseases. (biomedcentral.com)

The AnnoLnc program was used to perform an annotation analysis of lncRNAs. (jcancer.org)

Therefore, we were interested in early gene expression changes that might mediate neuroprotection in these mice. (jneurosci.org)

Gene expression can be studied at a genome-wide scale with the aid of modern microarray technologies. (open.ac.uk)

The intensity of fluorescence is analyzed and correlates with expression of the gene. (medscape.com)

We have modified a previously published meta-analysis method and identified a common metastatic signature by comparing primary tumors versus metastases in various tumor types. (nih.gov)

We hypothesize that a meta-analysis of publicly available gene expression datasets in various tumor types can identify a signature of metastasis that is common to multiple tumor types. (nih.gov)
To study in detail the relation between gene expression and resistance against gemcitabine, a cell line was isolated from a tumor for which gemcitabine resistance was induced in vivo. (amsterdamumc.org)
Although our analysis was limited to penile SCC, the rationale should also hold for other tumor types in which evaluation of p53 immunohistochemical expression is required. (gov.py)

Both genetics and environmental factors influence the expression of asthma, and not all asthma is the result of a specific immune response to allergen. (cdc.gov)

Unsupervised machine learning approaches provide new and efficient analysis of gene expression profiles. (biomedcentral.com)
This review focuses on microarray-based approaches for studying transcription response because they hold exceptional promise for the study of infectious disease. (medscape.com)

Cite this: Using DNA Microarrays to Study Host-Microbe Interactions - Medscape - Sep 01, 2000. (medscape.com)

Anatomy2

Organisms2

Chemicals and Drugs7

Analytical, Diagnostic and Therapeutic Techniques and Equipment14

Phenomena and Processes17

Disciplines and Occupations3

Information Science13

Health Care4

Genes60

Affymetrix7

High-throughput5

Tissue11

Hybridization5

Dataset2

MRNA4

Suggest that microarray2

Immunohistochemistry1

Pathways5

Methods7

Data25

Computational1

Transcriptome2

Whole-genome1

Pathway4

Experiments2

Patterns4

Assay1

Genetic5

Probes4

Protein expression1

Endothelial cells1

Functional2

Technology7

Chromatin1

Profiling7

Hypothalamic1

Results3

Differential expression1

Changes in gene expression1

Biomarker1

Arrays3

Biological1

Annotation1

Gene expression changes1

Genome-wide1

Fluorescence1

Comparing primary1

Tumor3

Genetics1

Approaches2

20001