• Using a construct with both the human alpha- and beta-globin genes and the beta-globin DCR, live mice with low-copy numbers were obtained. (ox.ac.uk)
  • Both human globin genes are expressed at high levels in adult red cells to give human hemoglobin HbA in amounts equal to or greater than endogenous mouse hemoglobin. (ox.ac.uk)
  • Metabolic labeling experiments showed balanced mouse globin synthesis, but imbalanced human globin synthesis, with an alpha/beta biosynthetic ratio of approximately 0.6. (ox.ac.uk)
  • Expression in fetal liver and blood is copy number dependent and at levels comparable to that of the endogenous mouse alpha-globin genes. (ox.ac.uk)
  • An important goal of the research community is to translate information gained from studying transgenic mouse models into useful clinical applications. (aacrjournals.org)
  • Reprogramming cells within live mice yields a new type of induced pluripotent stem cell. (the-scientist.com)
  • Correcting diastolic dysfunction by Ca 2+ desensitizing troponin in a transgenic mouse model of restrictive cardiomyopathy," Journal of Molecular and Cellular Cardiology , vol. 49, no. 3, pp. 402-411, 2010. (hindawi.com)
  • Abnormal cardiac inflow patterns during postnatal development in a mouse model of Holt-Oram syndrome," American Journal of Physiology , vol. 289, no. 3, pp. (hindawi.com)
  • Unlike HuCII- and HuCIII-transgenic mice, VLDL from HuCI transgenic mice bound heparin-Sepharose, a model for cell-surface glycosaminoglycans, normally. (jci.org)
  • It is unclear what relevance these mice may have for studies of Alzheimer's disease, but it is an interesting model of tau-mediated cell dysfunction in the hippocampus. (alzforum.org)
  • In this study, we examined collateral formation in peripheral arterial disease (PAD) model in Lp(a) transgenic mice. (ahajournals.org)
  • We characterized the kinetic properties of system X AG (sodium-dependent) and systems x c- (sodium-independent) [3H]- l -glutamate uptake in the striatum and hippocampus of HIV-1 gp120 transgenic mice, an established model of HIV neuropathology. (springer.com)
  • To model this mutation in mice, we have generated Disc1 tr transgenic mice expressing 2 copies of truncated Disc1 encoding the first 8 exons using a bacterial artificial chromosome (BAC). (jneurosci.org)
  • Further, fingolimod (a sphingoshine 1 phosphate receptor modulator) rescues reduced levels of BDNF in several regions of the brain including the striatum and hippocampus, and ameliorates symptoms in a mouse model of Rett syndrome 11 . (nature.com)
  • These results support the use of PyV-mT transgenic mice as a model for investigating the multistep progression of malignant mammary tumorigenesis and metastasis. (aacrjournals.org)
  • To develop the model, the investigators will create "transgenic" mice with both human tau, and a "bioluminescent" imaging reporter that is regulated by the formation of tau prions. (dana.org)
  • A mouse model that closely models the human disease would, therefore, be of significant benefit. (bloodjournal.org)
  • To generate a mouse model for slow progressive retinal neovascularisation through vascular endothelial growth factor (VEGF) upregulation. (ovid.com)
  • These chimeric mice have been developed as a mouse model of human hypertension induced by activation of the human RAS. (ahajournals.org)
  • So, we believe that Tg972 mice may represent a useful model to elucidate the interaction between genetic and environmental factors in insulin resistance pathogenesis. (sigmaaldrich.com)
  • CONCLUSIONS: These mice represent an animal model system for human trilateral retinoblastoma, in which retinoblastomas are accompanied by pineal tumors. (arvojournals.org)
  • Publishing their results preliminarily on the open source journal BioRxiv , researchers at Texas Biomed were among a limited group of scientists nationwide given early access to newly developed transgenic mice that express the human angiotensin-converting enzyme 2 (hACE2) to test whether these mice can be used as a small animal model to assess SARS-CoV-2 infection. (newswise.com)
  • Researchers at Texas Biomed not only found that the human transgenic mice serve as a good model for SARS-CoV-2 infection but also discovered key indicators of an inflammatory immune response (activation of the immune system). (newswise.com)
  • In addition to establishing the K18 hACE2 transgenic mouse model supplied by The Jackson Laboratory, researchers also found these transgenic mice were the most susceptible to severe COVID-19 disease progression and produced what is known as a chemokine storm. (newswise.com)
  • Lymph Node Transplantation Decreases Swelling and Restores Immune Responses in a Transgenic Model of Lymphedema. (cnio.es)
  • Toxicity of chlorpyrifos and chlorpyrifos oxon in a transgenic mouse model of the human paraoxonase (PON1) Q192R polymorphism. (cdc.gov)
  • A metabolomic study of the CRND8 transgenic mouse model of Alzheimer's disease. (biomedsearch.com)
  • This study engineered an hCAR-BAC-transgenic (hCAR-TG) mouse model by integrating the 8.5-kbp hCAR gene as well as 73-kbp upstream and 91-kbp downstream human genomic DNA into the genome of CAR-null mice. (ovid.com)
  • This proto-oncogene has been overexpressed in a mouse model of the disease in order to explore the role of MYCN in this tumour. (bmj.com)
  • These studies highlight the histological similarities between tumours from MYCN transgenic mice and human neuroblastomas, and reaffirm their role as a valuable model to study the biology of aggressive human neuroblastoma. (bmj.com)
  • To facilitate studies of podocyte function, we have developed a transgenic mouse model with inducible expression in the podocyte. (asnjournals.org)
  • Since their initial generation in the mid 1990s, transgenic mouse models of Alzheimers's disease (AD) have been proven to be valuable model systems which are indispensable for modern AD research. (pubmedcentralcanada.ca)
  • Effects of Human Alpha-Synuclein A53T-A30P Mutations on SVZ and Local Olfactory Bulb Cell Proliferation in a Transgenic Rat Model of Parkinson Disease. (genoway.com)
  • Mice are a useful model for genetic manipulation and research, as their tissues and organs are similar to that of a human and they carry virtually all the same genes that operate in humans. (wikipedia.org)
  • Previous attempts to culture PGCs from mice and humans produced embryonic germ cells that look and act like embryonic stem cells. (rxpgnews.com)
  • The panel recommended that diagnostic nomenclature of transgenic lesions in mice be based on similar morphological, immunohistochemical, and biological criteria so that direct comparisons among mouse models and with humans would be possible. (aacrjournals.org)
  • What makes these mice particularly interesting, say the researchers, is that they exhibit sex differences in the development of cardiac hypertrophy similar to those in humans. (bio-medicine.org)
  • In this study, scientists showed the K18 hACE2 transgenic mice not only succumbed to the infection but showed systemic (multiple organ) infection with significant virus detected in the nasal passages, lungs, and brain - all organs greatly impacted by SARS-CoV-2 in humans. (newswise.com)
  • and (3) major hCAR mRNA SVs increase markedly in postnatal livers of hCAR-TG mice, which mimics the ontogeny of CAR mRNA in humans. (ovid.com)
  • Transgenic mouse lines were established that routinely develop these tumors, and they should provide a valuable resource for studies involving cardiac and bone physiology and neoplasia. (pnas.org)
  • The need for additional transforming events, suggested by the low incidence of spontaneous tumors, was further indicated by the high susceptibility of the transgenic animals to injections of low doses of N-methyl-N-nitrosourea, a chemical carcinogen with a thymic tropism. (nih.gov)
  • Although numerous molecular insights were gained from the analysis of these transgenic malignant tumors, the early events leading to malignant transformation have not been systematically investigated nor has the biological potential of hyperplastic lesions been documented. (aacrjournals.org)
  • These mice have a higher incidence of both induced and spontaneous tumors, resulting in increased mortality during the first year of life. (biomedsearch.com)
  • Intracamerally transplanted transgenic tumors metastasized from the eyes to the livers of nude mice. (arvojournals.org)
  • CONCLUSIONS: In TySV40 transgenic mice, intraocular tumors develop that arise at the choroid-RPE interface, and they display morphologic and ultrastructural features consistent with RPE carcinomas. (arvojournals.org)
  • However, the transgenic tumors express melanoma-associated antigens and a propensity to metastasize to the liver, two features characteristic of uveal melanomas. (arvojournals.org)
  • These mice have increased sensitivity to developing carcinogen-induced tumors, and by using this mouse strain instead of conventional ones, reductions can be made in both timelines and the number of animals required. (criver.com)
  • Photoreceptor cell tumors in transgenic mice. (arvojournals.org)
  • RESULTS: All mice produced developed either ocular or intracranial tumors, or both, at an early age. (arvojournals.org)
  • One line of mice was generated, and all mice of this line develop both retinal photoreceptor cell and pineal tumors by as early as 2 weeks of age. (arvojournals.org)
  • Using skin-targeted transgenic mice, she demonstrated that elevated levels of ODC play a causal role in the development of tumors. (wikipedia.org)
  • Taking their findings one step further, Gilmour and her team in 2017 demonstrated that a polyamine blockade therapy (PBT) they developed inhibited the growth of malignant tumors in mice and activated an immune response to those tumors. (wikipedia.org)
  • In an attempt to determine the susceptibility of haploid, round spermatids to neoplastic transformation by this oncogene, transgenic mice were generated that harbored a chimeric gene composed of the SV40 T-antigen genes fused to the 5' and 3' flanking sequences of the mouse protamine 1 gene. (pnas.org)
  • Increasing the expression of two genes from non-Y chromosomes restores spermatogenesis in male mice that lack Y chromosomes. (the-scientist.com)
  • In a proof-of-principle study, genetic deletion of two genes renders male mice infertile by preventing sperm transport through the vas deferens. (the-scientist.com)
  • Palmiter RD, Chen HY and Brinster RL (1982) Differential regulation of metallothionein-thymidine kinase fusion genes in transgenic mice and their offspring. (springer.com)
  • The transgene and several skeletal muscle-specific genes were expressed only in patchy areas of the heart in heterozygous mice. (ahajournals.org)
  • Ectopic expression of MyoD in the hearts of transgenic mice activated some muscle-specific skeletal sarcomeric genes and resulted in embryonic lethality with severe cardiac abnormalities in fetuses heterozygous for the transgene. (ahajournals.org)
  • For this purpose, we used chimeric double transgenic (Tg) mice of the human renin (hRN) and human angiotensinogen (hANG) genes. (ahajournals.org)
  • The new mice were created using genetic engineering techniques that allow researchers to disable specific genes in an animals genome. (bio-medicine.org)
  • The antigen-binding domains of HLA-DRA and HLA-DRB1*0401 (representative of the DR4 supertype) were attached to the membrane-proximal domains of I-Ed alpha and I-Ed beta (H2-E), respectively, by replacing exon 2 of the mouse genes with exon 2 of the human genes. (taconic.com)
  • Genetically modified mice are commonly used for research or as animal models of human diseases, and are also used for research on genes. (wikipedia.org)
  • In 1974 Rudolf Jaenisch created the first genetically modified animal by inserting a DNA virus into an early-stage mouse embryo and showing that the inserted genes were present in every cell. (wikipedia.org)
  • This method creates a transgenic mouse and is used to insert new genetic information into the mouse genome or to over-express endogenous genes. (wikipedia.org)
  • On the FVB genetic background, homozygous transgenic animals fed a high-fat diet ate 10% more and were 12% heavier at 13 weeks of age than wild-type animals, and they had higher systemic leptin levels. (jci.org)
  • While that earlier research was done with chimeric chickens, it demonstrated the enormous opportunities that transgenic chickens hold as a therapeutics production system," said Robert Kay, Ph.D. Origen Therapeutics president and chief executive officer. (rxpgnews.com)
  • We examined the possibility that continuous activation of the human brain renin-angiotensin system causes cognitive impairment, using human renin (hRN) and human angiotensinogen (hANG) gene chimeric transgenic (Tg) mice. (ahajournals.org)
  • The chimeric (hRN/hANG-Tg) mice were produced by mating of hRN-Tg and hANG-Tg mice. (ahajournals.org)
  • Since FGF-1 lacks a classical signal peptide consensus sequence, transgenic mice were also produced with a chimeric construct containing the signal peptide sequence of the FGF-4 gene fused in frame to the coding sequences of the FGF-1 cDNA in order to obtain extracellular expression of the transgene. (biologists.org)
  • Moreover, these Tg mice showed increased susceptibility to the infection with an intracellular pathogen, blood-stage Plasmodium berghei XAT, which is an irradiation-induced attenuated substrain of P. berghei NK65, presumably due to the decreased IFN-γ production. (jimmunol.org)
  • To elucidate pathological roles of the virus in disease, transgenic mice were produced that carry the HTLV-I genome. (sciencemag.org)
  • 1. A transgenic mouse, whose genome comprises an indicator gene under the control of a transcriptional regulatory element, wherein said transcriptional regulatory element comprises a MEF2 binding site and said indicator gene is (a) expressed in embryonic cardiac tissue, (b) not expressed in adult cardiac tissue, and (c) expressed in adult cardiac tissue in response to hypertrophic signals. (google.com)
  • The HBV Tg05 mouse line, which contains the 1.3-mer HBV genome and productively replicates HBV in the liver ( 21 ), was crossed with the mouse line that contains the floxed Atg5 gene (Atg5 f/f ). (asm.org)
  • A genetically modified mouse (Mus musculus) is a mouse that has had its genome altered through the use of genetic engineering techniques. (wikipedia.org)
  • The first involves pronuclear injection into a single cell of the mouse embryo, where it will randomly integrate into the mouse genome. (wikipedia.org)
  • The implantation of the Btc transgenic mice was delayed, but this was not the reason for the litter size reduction, because the mean number of total embryos either attached or recovered from the uterus of transgenic females was already markedly reduced when compared to the number of embryos present in the uterus of control females. (uni-muenchen.de)
  • Expression of the transgene was associated with an increase in Ca 2+ -stimulated myofibrillar ATPase in myofibrils obtained from the left ventricles of 42-day-old bmyf5 mice. (ahajournals.org)
  • Combined hyperlipidemia in transgenic mice overexpressing human apolipoprotein Cl. (jci.org)
  • We have generated transgenic mice over-expressing human apolipoprotein CI (apo CI) using the native gene joined to the downstream 154-bp liver-specific enhancer that we defined for apo E. Human apo CI (HuCI)-transgenic mice showed elevation of plasma triglycerides (mg/dl) compared to controls in both the fasted (211 +/- 81 vs 123 +/- 52, P = 0.0001) and fed (265 +/- 105 vs 146 +/- 68, P (jci.org)
  • BAFF was also independently identified as TALL-1 ( 24 ), as THANK, which regulated apoptosis, NF-κB and c-Jun NH 2 -terminal kinase in a human myelocytic cell line ( 25 ), and, more recently, as BlyS, a factor that, when administrated to normal mice, disrupted splenic B and T cell zones and resulted in elevated serum IgM concentrations ( 26 ). (rupress.org)
  • Here we document the age-related patterns in learning ability of TGM and normal mice. (chiro.org)
  • Learning appeared inferior in both genotypes of very young mice but TGM were confirmed to be superior to normal mice upon maturity. (chiro.org)
  • Older TGM, however, showed rapid age-related loss of their exceptional learning, whereas normal mice at 1 year of age showed little change. (chiro.org)
  • In Tg(PrP,ΔGPI) mice, disease onset could be accelerated either by inoculation with brain homogenate prepared from spontaneously ill animals or by coexpression of membrane-anchored, full-length PrP C . In contrast, coexpression of N-terminally truncated PrP(Δ23-88) did not affect disease progression. (pnas.org)
  • Remarkably, disease from ill Tg(PrP,ΔGPI) mice transmitted to mice expressing wild-type PrP C , indicating the spontaneous generation of prions. (pnas.org)
  • Though the requirement of PrP C in disease pathogenesis is known, the function of PrP C in nondiseased tissue remains enigmatic because Prnp 0/0 mice exhibit only subtle or disputed phenotypic deficits, arguing that PrP C is largely dispensable for proper neuronal function ( 3 ). (pnas.org)
  • Disease resistance in DQ6 transgenic mice was associated with reduced synthesis of anti-AChR IgG, IgG2b, and IgG2c Ab's and reduced IL-2 and IFN-γ secretion by H-AChR- and peptide α320-337-specific lymphocytes. (jci.org)
  • A proportion of A51 mice spontaneously develop AIG by 10 wk of age, with a disease characterized by eosinophilic infiltration of the gastric mucosa and Th2 differentiation of transgenic T cells in the gastric lymph node. (jimmunol.org)
  • In contrast, autoantigen-specific Th2 T cell clones, when transferred to T cell-deficient mice, lead to the development of disease in both insulin-dependent diabetes and EAE models ( 8 , 9 ). (jimmunol.org)
  • Autoimmune gastritis (AIG) is commonly observed in d3Tx BALB/c mice and resembles the human disease pernicious anemia, in that the effector T cells and autoantibodies recognize the α and β subunits of the gastric parietal cell H/K-ATPase ( 11 , 12 ). (jimmunol.org)
  • VavP- Bcl2 mice also had a propensity to develop kidney disease of the autoimmune type. (bloodjournal.org)
  • While minor differences in weight fluctuation were noted between male and female mice after infection, the study did not indicate that gender played a major role in SARS-CoV-2 infectivity or disease progression. (newswise.com)
  • This study is the first to identify the amount of virus necessary during an initial exposure for the transgenic mice to develop severe COVID-19 disease. (newswise.com)
  • Mice culled at 344 and 468 d had clinical prion disease. (cdc.gov)
  • The mouse culled at 720 d had clinical prion disease. (cdc.gov)
  • Two mice with clinical prion disease were culled at 558 and 749 d. (cdc.gov)
  • All mice had clinical prion disease. (cdc.gov)
  • In contrast, Tg mice expressing OvPrP with valine (V) at 136 (OvPrP-V136) infected with the same prions developed disease rapidly, and the converted prion was comprised of an unstable (U), diffusely distributed conformer. (nih.gov)
  • Percent (%) affected mice refers to numbers of mice within an inoculated cohort manifesting progressive clinical signs associated with prion disease. (nih.gov)
  • We have extensive experience and a strong track record in the design of mouse models for inflammatory diseases such as hepatitis, nephritis or inflammatory bowel disease, autoimmune diseases such as systemic lupus erythematosus, joint disorders such as arthritis, allergy , and infections such as cholera, hepatitis, leishmaniasis or chlamydial infection. (genoway.com)
  • To directly investigate the therapeutic potential of DCR3 in preventing this disease, we generated transgenic nonobese diabetic mice, which overexpressed DCR3 in beta cells. (sigmaaldrich.com)
  • Genetically modified mice are used extensively in research as models of human disease. (wikipedia.org)
  • The disease symptoms and potential drugs or treatments can be tested against these mouse models. (wikipedia.org)
  • Using FISH and genetic analyses, the transgenic insert causing the crsp mutation has been mapped to the distal part of mouse chromosome 5. (nih.gov)
  • In 1981 the laboratories of Frank Ruddle from Yale University, Frank Costantini and Elizabeth Lacy from Oxford, and Ralph Brinster and Richard Palmiter in collaboration from the University of Pennsylvania and the University of Washington injected purified DNA into a single-cell mouse embryo utilizing techniques developed by Brinster in the 1960s and 1970s, showing transmission of the genetic material to subsequent generations for the first time. (wikipedia.org)
  • Comprehensive and authoritative, Transgenic Mouse: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this evolving field. (springer.com)
  • A transgenic mouse company is paying researchers who mention its animal models in scientific papers. (the-scientist.com)
  • Unlike the human apo CII (HuCII)- and apo CIII (HuCIII)-transgenic mouse models of hypertriglyceridemia, plasma cholesterol was disproportionately elevated (95 +/- 23 vs 73 +/- 23, P = 0.002, fasted and 90 +/- 24 vs 61 +/- 14, P (jci.org)
  • That raises the question of whether the pathological features seen in AD mouse models arise from AICD, or Aβ, or both. (alzforum.org)
  • We have looked at three mouse models so far and we do see increased AICD levels," Pimplikar told ARF. (alzforum.org)
  • Is the Subject Area "Mouse models" applicable to this article? (plos.org)
  • 5 The tumorigenic potential of Bcl2 has been explored in transgenic mouse models that mimic the translocation by expressing the gene in B cells under the control of an Igh enhancer (Eμ). (bloodjournal.org)
  • I have no experience working with mice models, but why, for example, in pronuclear injection (or even other methods of transgenesis), is the injected gene of interest randomly integrated? (physicsforums.com)
  • This review focuses on the insights gained from transgenic mouse models. (frontiersin.org)
  • Mouse models have enhanced our understanding of (patho)-physiological implications of Cx diversity in the heart. (frontiersin.org)
  • 8, 9 ] Transgenic animals with alterations of the growth hormone axis are promising models of brain structure, function, and age-related changes relevant to all of these interventions. (chiro.org)
  • Taconic Transgenic Models™ (Models) are produced and distributed under rights to patents and intellectual property licensed from various institutions. (taconic.com)
  • Scientists have not previously had access to mouse models for SARS-CoV-2 infection studies, because typical lab mice are not susceptible to this virus. (newswise.com)
  • Identification of the underlying mutations opened manifold opportunities for the generation of transgenic mouse models. (pubmedcentralcanada.ca)
  • We have extensive experience and a strong track record in the design of mouse models for neuronal diseases such as Alzheimer's , Parkinson's , retinitis or encephalomyelitis , behavioral disorders such as autism , schizophrenia , depression or anxiety , and other research fields like epilepsy , stress , pain , taste or thermoreception . (genoway.com)
  • Using ODC transgenic mouse models, Gilmour showed that elevated ODC and polyamines promote tumorigenesis via multiple functional mechanisms including increasing proliferation, angiogenesis, chromatin remodeling and invasiveness, while also suppressing the immune response. (wikipedia.org)
  • MT-Exendin mice express proexendin-4 mRNA in multiple tissues and process the proexendin-4 precursor to Ex-4, resulting in detectable circulating levels of bioactive Ex-4 ( 11 ). (diabetesjournals.org)
  • No changes of HSPB8, BAG3, and BAG1 at PS stage (8 weeks) were seen in all tissues examined in tg G93A-SOD1 and control mice. (rug.nl)
  • Glucose utilization and insulin signalling were impaired in all key insulin target tissues in Tg972 mice. (sigmaaldrich.com)
  • the results shown are the mean of tissues derived from two mice. (asnjournals.org)
  • To determine whether the enhancement is a general outcome of splicing or is dependent on the particular intron, we also produced equivalent transgenics carrying the widely used simian virus 40 small-t intron. (asm.org)
  • The data published so far show that APOE*3-Leiden mice exhibit severe hypercholesterolemia when they are fed a high-fat/high-cholesterol (HFC) diet. (ahajournals.org)
  • Behaviorally, transgenic mice exhibit increased immobility and reduced vocalization in depression-related tests, and impairment in conditioning of latent inhibition. (jneurosci.org)
  • These mice develop a severe cardiomyopathy and exhibit a significantly shorter life span than do their nontransgenic littermates. (ahajournals.org)
  • Conclusions -This study shows that in APOE*3-Leiden mice, duration of an HFC diet is associated with (1) a craniocaudal progression of lesion development and (2) an increased complexity of atherosclerotic lesions. (ahajournals.org)
  • Affected male mice had degenerative testicular lesions, and their sperm was immotile. (ingentaconnect.com)
  • Nonpolycystic K14 control male mice bred well, had no testicular lesions, and had appropriate sperm motility. (ingentaconnect.com)
  • TXA23, a Th1 cell, induces, on transfer to nu/nu mice, a severe gastritis characterized by a lymphocytic infiltrate and autoantibody production. (jimmunol.org)
  • ATXN2-CAG42 sequesters PABPC1 into insolubility and induces FBXW8 in cerebellum of old ataxic knock-in mice. (genoway.com)
  • Pavirani A, Skern T, Le Meur M, Lutz Y, Lathe R, Crystal RG, Fuchs JP, Gerlinger P and Courtney M (1989) Recombinant proteins of therapeutic interest expressed by lymphoid cell lines derived from transgenic mice. (springer.com)
  • The ability to readily create transgenic chickens through this technology, and then to scale up production through conventional breeding further adds to the practicality of this technology for large-scale production of therapeutic proteins. (rxpgnews.com)
  • Previous work showed no evidence of increased mRNA or transcriptional activation of the GSK3β gene in the mice, despite an increase in kinase activity. (alzforum.org)