Intracytoplasmic Sperm InjOocyteMammalian spermFertilizationMaturationGenetic MutationsMotilityHumansAcrosomalBiologicalGenomeOccurMammalsZona pellucidaVivoInfertileEmbryosMeiosisSpermatozoaProteinActivationDosesReproductionDevelopmentMolecularBiologyRatsCellsVitroSpeciesMaleModelsEmbryonicIncreasesCellStudyReductionRoleImportantMultipleImmune
Intracytoplasmic Sperm Inj1
- This study was conducted to evaluate in vivo and in vitro development of in vitro-matured equine oocytes fertilized by intracytoplasmic sperm injection. (bioone.org)
Oocyte4
- Oocyte (or ovum/egg) activation is a series of processes that occur in the oocyte during fertilization. (wikipedia.org)
- Sperm entry causes calcium release into the oocyte. (wikipedia.org)
- IP3 and DAG are produced from the cleavage of PIP2 by phospholipase C. However, another hypothesis is that a soluble 'sperm factor' diffuses from the sperm into the egg cytosol upon sperm-oocyte fusion. (wikipedia.org)
- Once PLCζ is introduced into the oocyte by the sperm cell, it cleaves phospholipid phosphatidylinositol 4,5-bisphosphate (PIP2) into diacyl glycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). (wikipedia.org)
Mammalian sperm3
- A novel PLC isoform, PLCζ (PLCZ1), may be the equivalent of the mammalian sperm factor. (wikipedia.org)
- A 2002 study demonstrated that mammalian sperm contain PLC zeta which can start the signaling cascade. (wikipedia.org)
- Structural events and molecular mechanisms that promote mammalian sperm acrosomal exocytosis and motility. (uba.ar)
Fertilization6
- The acrosome, a single exocytotic vesicle on the head of sperm, has an essential role in fertilization, but the exact mechanisms by which it facilitates sperm-egg interactions remain unresolved. (nih.gov)
- Soon after fertilization, the vast majority of methylcytosine in sperm is converted by the Tet3 enzyme to hydroxymethylcytosine that gets lost by dilution during replication, effectively erasing cytosine methylation patterns except for a subset that is maintained, including those of some imprinted genes. (i-sis.org.uk)
- After fertilization, the sperm genome is rapidly stripped of protamines and most, but not all histones. (i-sis.org.uk)
- The minisymposium, organized by Victor D. Vacquier, included the following presentations: "An Hypothesis to Explain the Evolution of Species-Specific Fertilization" by Victor D. Vacquier, "Molecular Biology of the Zona Pellucida: Genetic Mutations and Fertility" by Jurrein Dean, and "Sperm-Egg Membrane Interactions During Fertilizations" by Janice P. Evans. (bioone.org)
- Transient exposure to calcium ionophore enables in vitro fertilization in sterile mouse models. (umassmed.edu)
- The tube, which connects the peritoneal space to the endometrial cavity, captures the egg after ovulation and transports the sperm from the uterus to the fertilization site in the ampulla (the middle portion of the tube). (medscape.com)
Maturation2
- Diagram showing the reduction in number of the chromosomes in the process of maturation of the ovum . (wikipedia.org)
- oocytogenesis , ootidogenesis , and finally maturation to form an ovum (oogenesis proper). (wikipedia.org)
Genetic Mutations1
- If it affects the genetic material of germ cells (the sperm and ova), then genetic mutations could be transmitted to the children of cannabis users. (druglibrary.org)
Motility2
- Due to the increased metabolic activity of the sperm compared to the egg, due to its motility, there is greater production of reactive oxygen species and therefore greater chance of mutation. (wikipedia.org)
- Calcineurin regulates progressive motility activation of Rhinella (Bufo) arenarum sperm through dephosphorylation of PKC substrates. (umassmed.edu)
Humans4
- Other recent studies verified the presence of PAPP-A mRNA in granulosa cells of humans, monkeys, cattle, mice, and pigs. (bioone.org)
- Mammalian oocytes are maintained in meiotic prophase arrest for a very long time-months in mice, years in humans. (wikipedia.org)
- These data demonstrate that MAP3K4-dependent signalling events are required for normal expression of Sry during testis development, and create a novel entry point into the molecular and cellular mechanisms underlying sex determination in mice and disorders of sexual development in humans. (plos.org)
- Animal models (e.g., mice, rats, zebrafish and others) are sufficiently like humans in their anatomy, physiology or response to a pathogen that researchers can extrapolate the results of animal model studies to better understand human physiology and disease. (genome.gov)
Acrosomal1
Biological1
- Analysis of procainamide-derived heparan sulphate disaccharides in biological samples using hydrophilic interaction liquid chromatography mass spectrometry. (mdx.ac.uk)
Genome5
- H3K9me2 was found at several paternally methylated imprinted regions in sperm, suggesting that this histone mark signals special sites of the paternal genome where methylation is maintained. (i-sis.org.uk)
- Advances in the biotechnology industry have increased scientists' understanding of the human genome and enhanced their ability to genetically modify eggs, sperm, and human embryos. (nyu.edu)
- We report here the whole-genome sequences of the human-infective Trichuris trichiura and the mouse laboratory model Trichuris muris . (nature.com)
- Here we present high-quality genome sequences for T. trichiura and T. muris , the first duo of a major human STH and its mouse counterpart. (nature.com)
- In parallel, multiple technologies and manual finishing were used to produce an 85-Mb reference genome assembly from the more readily available mouse parasite species T. muris (Online Methods and Supplementary Note ). (nature.com)
Occur1
- Sperm-egg interactions occur at multiple levels on the egg surface, first with the egg's extracellular matrix and then with the egg's plasma membrane. (bioone.org)
Mammals2
- In mammals, this is caused by the introduction of phospholipase C isoform zeta (PLCζ) from the sperm cytoplasm. (wikipedia.org)
- In mammals, most histone proteins are lost during sperm development, eventually to be replaced by protamines. (i-sis.org.uk)
Zona pellucida1
- ABSTRACT Anti-zona-pellucida autoantibodies (AZP-Ab) and anti-sperm isoantibodies (ASA) were as- sessed in the cervical secretions from 73 infertile Jordanian women and 41 fertile control women using latex agglutination. (who.int)
Vivo3
- The use of genetically engineered mouse models has yielded new mechanistic information that complements recent, important in vivo imaging studies. (nih.gov)
- Rossant, J. & Lis, W. T. Potential of isolated mouse inner cell masses to form trophectoderm derivatives in vivo . (nature.com)
- AIM OF THE STUDY: To study the effects of MEEP in vivo using a chronic ovalbumin (OVA)-induced allergic asthma model in mice. (bvsalud.org)
Infertile1
- Some gene knockouts produce infertile phenotypes with obviously missing, specific activities that affect acrosome biogenesis during spermatogenesis or interfere with acrosome function in mature sperm. (nih.gov)
Embryos1
- Rossant, J. & Vijh, K. M. Ability of outside cells from preimplantation mouse embryos to form inner cell mass derivatives. (nature.com)
Meiosis1
- Activation of the ovum includes the following events: Cortical reaction to block against other sperm cells Activation of egg metabolism Reactivation of meiosis DNA synthesis The sperm may trigger egg activation via the interaction between a sperm protein and an egg surface receptor. (wikipedia.org)
Spermatozoa1
- Additionally, quality control of spermatozoa is much worse than for the ovum, as many sperm are released whereas only one dominant follicle is released per cycle. (wikipedia.org)
Protein2
- The hyalin forms a layer adjacent to the plasma membrane and the peroxidases cross-link the protein in the vitelline membrane to harden it and make it impenetrable to sperm. (wikipedia.org)
- Here we report a role for the phylogenetically ancient mitogen-activated protein kinase (MAPK) signalling pathway in mouse sex determination. (plos.org)
Activation1
- This receptor is activated by the sperm binding and a possible signaling pathway could be the activation of a tyrosine kinase which then activates phospholipase C (PLC). (wikipedia.org)
Doses2
- Four different doses of plant extract were used in mice model. (bvsalud.org)
- We have demonstrated that, in certain conditions, an intravenous (i.v.) injection of PEGylated liposomes (PEG-Lip) induced anti-PEG IgM antibodies, which led to rapid clearance of second doses in mice. (matci.jp)
Reproduction1
- EDITOR'S PREFACE: The first paper in this issue of Biology of Reproduction is a minireview entitled "Getting Sperm and Egg Together: Things Conserved and Things Diverged," by Janice P. Evans. (bioone.org)
Development3
- Rossant, J. & Tam, P. P. L. Emerging asymmetry and embryonic patterning in early mouse development. (nature.com)
- Graham, C. F. & Lehtonen, E. Formation and consequences of cell patterns in preimplantation mouse development. (nature.com)
- We identified a mouse mutant that causes embryonic gonadal sex reversal: the development of ovaries in an XY embryo. (plos.org)
Molecular1
- Together, these genomes and associated functional data elucidate key aspects of the molecular host-parasite interactions that define chronic whipworm infection. (nature.com)
Biology3
- In the case of environmental toxins, Michael Skinner's reproductive biology lab at Washington State University Pullman in the United States first reported in 2005 that injecting pregnant rats with endocrine disruptor fungicide vinclozolin caused sperm abnormalities that persisted in the male progeny for at least 4 generations [4]. (i-sis.org.uk)
- T. muris is an established laboratory mouse model for human trichuriasis and shares with T. trichiura many aspects of its biology, including the specialized niche within the host. (nature.com)
- Results: The process of hormesis serves as a bridge between the biology of neuron-germ cell interactions on one hand, and the clinical relevance of these interactions on the other. (mdpi.com)
Rats1
- Subsequently, they found that insecticides DDT and permethrin, jet fuel, plastic additives phthalates and bisphenol A, and dioxin can all trigger trans-generational health effects in rats such as obesity and ovarian disease, and each resulted in a different pattern of methylation in sperm DNA, according to Skinner. (i-sis.org.uk)
Cells1
- It is true that the company was not attempting to patent actual sperm or egg cells, but merely facilitate a "preview" of unborn children. (nyu.edu)
Vitro1
- Oocytes were collected from slaughterhouse-derived ovaries, matured in vitro, and injected with frozen-thawed stallion sperm. (bioone.org)
Species1
- Furthermore, sperm are exposed to reactive oxygen species from leukocytes in the epididymis during transit. (wikipedia.org)
Male2
- Interplanetary travel will encompass greater periods of seclusion for deep-space teams and a heightened amount of interactions among male and female astronauts - although there is no assurance that astronauts will engage in sexual activity. (sciencepolicyjournal.org)
- Male Balb/c mice were sensitized and challenged with OVA and experimental animals were treated with MEEP (100, 250 or 500 mg/kg) while control animals were treated with dexamethasone (2 mg/kg) or normal saline. (bvsalud.org)
Models1
- Figure 1: Classic models for lineage segregation in the early mouse embryo. (nature.com)
Embryonic1
- Rossant, J. & Tam, P. P. L. Blastocyst lineage formation, early embryonic asymmetries and axis patterning in the mouse. (nature.com)
Increases1
- After contact with sperm, an influx of sodium ions increases the potential up to +20mV. (wikipedia.org)
Cell6
- Izumo is the sperm cell signal, that will trigger the egg receptor Juno. (wikipedia.org)
- Johnson, M. H. & Ziomek, C. A. The foundation of two distinct cell lineages within the mouse morula. (nature.com)
- Fleming, T. P. A quantitative analysis of cell allocation to trophectoderm and inner cell mass in the mouse blastocyst. (nature.com)
- Louvet-Vallee, S., Vinot, S. & Maro, B. Mitotic spindles and cleavage planes are oriented randomly in the two-cell mouse embryo. (nature.com)
- [1] is the differentiation of the ovum (egg cell) into a cell competent to further develop when fertilized. (wikipedia.org)
- The purpose of a hen's egg is to provide all the materials necessary to develop the one cell - created by the joining of a cock's sperm with a hen's ovum - into a complete chick with feathers, beak, legs, and tail. (drmcdougall.com)
Study1
- In this study, we investigated anti-PEG IgM production in mice following i.m., intraperitoneal (i.p.) and subcutaneous (s.c.) administration of PEG-Lip. (matci.jp)
Reduction1
- reduction of elevated mice body temperature was observed by 2.6 °F and 4.2 °F after 4 h by paracetamol and 400 mg/kg.bw extract respectively. (bvsalud.org)
Role2
- Piotrowska, K. & Zernicka-Goetz, M. Role for sperm in spatial patterning of the early mouse embryo. (nature.com)
- Anti-sperm any medication with potentially negative antibody (ASA) may also play a different effects on the rheological characteristics role in female infertility by interfering with of the mucus were stopped in the previous sperm migration through the female genital cycle. (who.int)
Important1
- Complex coordinated contractions of the musculature are thought to be important for movement of the ovum from the distal end to the proximal end of the tube, while at the same time aiding in the movement of sperm from the proximal end to the distal end of the oviduct. (medscape.com)
Multiple1
- Polyspermy is the condition when multiple sperm fuse with a single egg. (wikipedia.org)
Immune3
- Importantly, perturbations in the microbiome can lead to both metabolic and immune diseases, highlighting the importance of understanding the "healthy" human microbiome and its interaction with the host. (frontiersin.org)
- Using RNA sequencing data from whipworm-infected mice, we describe the regulated T helper 1 (T H 1)-like immune response of the chronically infected cecum in unprecedented detail. (nature.com)
- Gene expression data from mice with low-dose whipworm infection provide a detailed description of a regulated T H 1-like immune response to the infected cecum that is not limited to the immediate site of infection. (nature.com)