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  • C57BL
  • A fraction of CD31 + cells of BM mononuclear cells in C57BL/6 mice co-expressed well known stem cell markers including c-kit, Sca-1, and flk-1. (ahajournals.org)
  • METHODS: CD25+CD4+ T cells from CBA mice that were pretreated with C57BL/6 (B.6) blood (donor-specific transfusion, DST) and nondepleting anti-CD4 Ab (YTS 177) were cotransferred with naïve CBA CD25-CD4+"effector" T cells into CBA-rag-/- mice. (ox.ac.uk)
  • Six months after suture removal, corneas were either analyzed histomorphometrically for pathological corneal hem- and lymphangiogenesis (using LYVE-1 as a lymphatic endothelial specific marker and CD31 as a panendothelial marker) or penetrating keratoplasty was performed with C57BL/6 donors (all mice female). (arvojournals.org)
  • Male C57BL/6J mice 3 months old were divided into two groups: EPO group and control groups. (spandidos-publications.com)
  • Antibody
  • Here, we isolated stem cell antigen-1 positive cells from postnatal mouse heart by magnetic active cell sorting using an iron-labeled anti-mouse Sca-1 antibody, and cultured them long-term in vitro. (biomedcentral.com)
  • Although volociximab has already been tested in open label, pilot phase II clinical trials in melanoma, pancreatic and renal cell cancer, evaluation of the mechanism of action of volociximab has been limited because this antibody does not cross-react with murine α5β1, precluding its use in standard mouse xenograft models. (biomedcentral.com)
  • We generated a panel of rat-anti-mouse α5β1 antibodies, with the intent of identifying an antibody that recapitulated the properties of volociximab. (biomedcentral.com)
  • The affinity of antibody 339.1 for mouse integrin α5β1 was determined to be 0.59 nM, as measured by BIAcore. (biomedcentral.com)
  • tumor
  • By combining administration of an engineered mouse serum albumin/IL-2 fusion with an Fc fusion to an integrin-binding peptide (2.5F-Fc), significant survival improvements are achieved in three syngeneic mouse tumor models, including complete responses with protective immunity. (rupress.org)
  • protein
  • controls received an equal amount of Fc protein (n=10 per group). (arvojournals.org)
  • In a mouse heart-to-rat transplant model, where it is possible to distinguish graft (mouse) from systemic (rat) chemokines, we show that donor PAR-1 is required to generate the local monocyte chemoattractant protein (MCP)-1 needed to recruit rat natural killer cells and macrophages into the hearts. (rupress.org)
  • Here we use a model of IFNγ induction and an IFNγ-GFP fusion protein in large, vascularized tumours growing in mice that express the IFNγ receptor exclusively in defined cell types. (nih.gov)
  • Using an antiserum to the vesicular acetylcholine transporter (VAChT), a marker for cholinergic neurons, many unusually large VAChT-immunoreactive (-ir) nerve terminals, identified by colocalization with the synaptic vesicle protein synaptophysin, were demonstrated in the hypothalamic arcuate nucleus of obese tub/tub mice. (strategian.com)
  • To test the hypothesis that nonhematopoietic stem cells in the BM are the primary source of marrow-derived lung epithelial cells, either wild-type hematopoietic or nonhematopoietic BM cells were transplanted into irradiated surfactant-protein-C (SPC)-null mice. (nih.gov)
  • transgenic
  • In the current study, we have used the Hi-Myc transgenic mouse model ( 21 ) of prostate cancer to explore the effects of dietary energy balance manipulation, including a standard diet-induced obesity (DIO) regimen, a control diet regimen that results in an overweight phenotype, and a calorie restriction (CR) regimen that leads to a lean phenotype, on prostate cancer progression. (aacrjournals.org)
  • Working in a model of acute humoral xenograft rejection (mouse heart into rat), we previously reported that inhibiting thrombin generation or inhibiting thrombin itself inside the graft (using organs from transgenic [Tg] mice expressing endothelial cell [EC]-tethered anticoagulants) completely inhibited humoral rejection so that hearts were rejected by infiltrating T lymphocytes ( 5 ). (rupress.org)
  • Expression of Cre recombinase under the hematopoietic vav-promoter leads to excision of the Stop-codon with subsequent expression of YFP in vav-Cre/ROSA26R-YFP (vav-YFP) transgenic mice. (nih.gov)
  • Hematopoietic (YFP positive) or nonhematopoietic (YFP negative) BM cells were sorted by flow cytometry from vav-YFP transgenic mice and transplanted into irradiated SPC-KO recipient mice. (nih.gov)
  • density
  • Probucol alone and probucol and cilostazol significantly decreased total, low-density lipoprotein, and high-density lipoprotein cholesterol, whereas cilostazol did not affect the plasma cholesterol levels in ApoE KO mice. (aspetjournals.org)
  • diabetic
  • Methods and Results We transplanted p53 and p21 KO mouse EPC s ( mEPC s) into streptozotocin-induced diabetic (type 1 diabetes mellitus model) C57 BL /6J and db/db (B6. (ahajournals.org)
  • Enhanced blood flow was noted with delivery of p53 and p21 KO mEPC s in streptozotocin ‐induced diabetic C57 BL /6J mice. (ahajournals.org)
  • Similar results were obtained when human Ad‐p53sh EPC s( CD 34+) and Ad‐p21sh EPC s( CD 34+) were transplanted into streptozotocin‐induced nonobese diabetic severe combined immunodeficiency mice. (ahajournals.org)
  • However, such beneficial effect was not noted in the db/db type 2 diabetic mouse models. (ahajournals.org)
  • In this study, healing rates of type II diabetic mice wounds were evaluated when human MSCs were delivered within a collagen scaffold (IntegraTM) and treated with Timolol, a beta blocker that inhibits the effects of epinephrine. (calpoly.edu)
  • vivo
  • of poly(I:C), LPS, or imiquimod did not modify the number of tetramer+ cells recovered from the spleens or LN at these early time points compared with mice immunized with peptide alone (data not shown), demonstrating a selective potency of CpG to enhance the early expansion of CD8+ T cells in response to soluble peptide in vivo. (cd31-signal.com)
  • spleen
  • The effects of CpG were not as striking PLX4032 mouse in the spleen, though similar trends were observed. (cd31-signal.com)
  • Curiously, antigen-specific IFN-γ secreting T cells were not detected in the spleen when immunizing mice with either peptide alone or CpG with peptide. (cd31-signal.com)
  • tumour
  • Mice were randomised in therapy and control groups when tumour size reached 100 +/- 20 mm3. (egms.de)
  • Dox-induced IFN γ expression in established MCA313 IFN γ -IND tumours (2nd panel) leads to tumour regression in wild-type but not IFN γ R − mice (3rd, 4th panel). (nih.gov)
  • hematopoietic
  • E): Lungs from SPC-KO mice transplanted with vav-YFP positive, hematopoietic BM cells contain vav-YFP positive blood cells. (nih.gov)
  • wild-type
  • IFN γ induction in wild-type mice at 642 ± 236 mm 3 (the two crosses indicate mice that reached a humane endpoint and were taken out of the experiment) and in IFN γ R − mice at 608 ± 130 mm 3 . (nih.gov)
  • F): Lung from SPC-KO mouse transplanted with wild-type HSPC. (nih.gov)
  • tissue
  • Blood vessel biomarkers GSL-I Isolectin B4 and CD31 were used to stain the wound tissue and fluorescent imaging data was quantified using software. (calpoly.edu)
  • Additionally, a Fluorescent in situ Hybridization (FISH) protocol to identify human chromosomes was successfully implemented using positive and negative control slides so that human MSCs can be identified when delivered to mouse wound tissue. (calpoly.edu)
  • Molecular
  • The aims of this study are to identify the role of PlGF in liver inflammation and fibrosis induced by bile duct ligation (BDL) in mice and to reveal the underlying molecular mechanism. (frontiersin.org)
  • monocyte
  • In view of the heightened appreciation of the heterogeneity of circulating monocytes, we investigated whether distinct monocyte subsets contribute in specific ways to myocardial ischemic injury in mouse MI. (rupress.org)
  • cells
  • CD31, PECAM-1 has been used as a marker for endothelial cells. (ahajournals.org)
  • We hypothesized that CD31 could serve as a comprehensive epitope to encircle various subsets of hemangioblastic cells in adult bone marrow (BM). (ahajournals.org)
  • All mice transplanted with 1x10 5 CD31 + cells survived after lethal irradiation, whereas mice with CD31 − cells all died within 4 weeks (n=10, each). (ahajournals.org)
  • We then tested therapeutic efficacy of CD31 + cells. (ahajournals.org)
  • The injected CD31 + cells preferentially assumed pericytic morphology. (ahajournals.org)
  • We demonstrated that CD31 antigen can function as a comprehensive marker to encompass most of the currently identified BM cells showing hemangioblastic activity. (ahajournals.org)
  • Some animals received 6x10(5) CD25+CD4+ T cells from pretreated mice to augment regulation on day -1. (ox.ac.uk)
  • We hypothesized that infiltration of NK cells and MΦs into rejecting mouse hearts was due to the establishment of a chemokine gradient, most likely MCP-1, a CC chemokine known to be essential for NK cell and MΦ recruitment. (rupress.org)
  • reference 7 ) and showed that mouse MCP-1 acted as a chemoattractant for rat cells (not depicted). (rupress.org)
  • BxPC-3 pancreatic cancer cells (5 x 106 in 0.2 ml RPMI 1640 medium were implanted subcutaneously (s.c.) in the dorsa of the mice (n=5/group). (egms.de)
  • Trp53 +/− mice, but not by nonvasculogenic glioma cells. (aacrjournals.org)
  • However, the number of antigen-specific cells recovered at day 5 was not different between CpG-treated and control mice. (cd31-signal.com)
  • However, antigen-specific Parvulin cells from the dLN of mice treated with CpG and peptide were readily detected by IFN-γ ELISPOT. (cd31-signal.com)
  • C) and (D): Lungs from two different SPC-KO mice that received nonhematopoietic BM cells contain donor-derived type 2 pneumocytes (vesicular cytoplasmic SPC and nuclear TTF1) located at the alveolar junction (arrows). (nih.gov)
  • weeks
  • Middle cerebral artery occlusion resulted in significantly larger infarct volumes in ApoE KO mice fed 10 weeks of high-fat diet compared with those in ApoE KO mice fed a regular diet. (aspetjournals.org)
  • Three interrupted 11-0 sutures were placed into the corneal stroma of BALB/c mice and left in place for 2-6 weeks. (arvojournals.org)
  • The mice were sacrificed at 2, 4, 6 and 8 weeks following the initiation of treatment. (spandidos-publications.com)
  • Immunohistochemical analysis suggested that EPO treatment up‑regulated the expressions of Runx2, osteocalcin, VEGF and CD31 at 4 and 8 weeks. (spandidos-publications.com)
  • Results
  • MI in atherosclerotic mice with chronic Ly-6C hi monocytosis results in impaired healing, underscoring the need for a balanced and coordinated response. (rupress.org)
  • growth
  • Collectively, these findings suggest that enhanced growth factor (Akt/mTORC1 and STAT3) and inflammatory (NFκB and cytokines) signaling may play a role in dietary energy balance effects on prostate cancer progression in Hi-Myc mice. (aacrjournals.org)
  • days
  • Participants will receive fludarabine and cyclophosphamide on days 1, 2, and 3 of six 28-day cycles. (stanford.edu)
  • Mice in the treatment group received VEGF Trap (25 mg/kg, intraperitoneally) on the day of keratoplasty as well as 4, 7 and 14 days later. (arvojournals.org)
  • We examined wounded mice after 7 days that had received either no MSCs (control), MSCs, or MSCs treated with timolol for blood vessel development using immunohistochemical staining and confocal fluorescence microscopy. (calpoly.edu)
  • Vascular
  • The presence of abnormal cholinergic/GABAergic vascular innervation in the arcuate nucleus suggests that alterations in this region, which contains neurons that receive information from the periphery and which relays information about the energy status to other parts of the brain, may be central in the development of the obese phenotype in animals with an autosomal recessive mutation in the tub gene. (strategian.com)
  • blood
  • Such large cholinergic and GABAergic nerve terminals surrounding blood vessels were not seen in the arcuate nucleus of lean tub/+ mice. (strategian.com)
  • prevents
  • The data suggest that the combination of probucol and cilostazol prevents cerebrovascular damage in focal cerebral ischemic mice with hypercholesterolemia by up-regulation of endothelial nitric oxide synthase and adiponectin. (aspetjournals.org)