• Despite their crucial role as a link between innate and adaptive immunity, little is known about the modulation of pDC differentiation by other bone marrow (BM) cells. (nih.gov)
  • In this study, we investigated the modulation of pDC differentiation in Flt-3 ligand (Flt3L)-supplemented BM cultures, using highly purified mesenchymal stem cells (MSCs) that were FACS-isolated from murine BM based on surface marker expression and used after in vitro expansion. (nih.gov)
  • The expression of CSF-1R, a receptor important for myeloid cell migration, differentiation and survival, and the effect of its therapeutic blockade in the context of GVAX in PDAC has not been investigated. (bmj.com)
  • Cyclin-dependent kinase 2-associating protein 1 commits murine embryonic stem cell differentiation through retinoblastoma protein regulation. (nih.gov)
  • Together, our results provide first evidence for a specific effect of [environmental enrichment] on T cell differentiation and its associated changes in gene expression profile," the authors wrote in their paper. (the-scientist.com)
  • Here we present SR1001, a high-affinity synthetic ligand-the first in a new class of compound-that is specific to both RORα and RORγt and which inhibits T H 17 cell differentiation and function. (nature.com)
  • Our data demonstrate the feasibility of targeting the orphan receptors RORα and RORγt to inhibit specifically T H 17 cell differentiation and function, and indicate that this novel class of compound has potential utility in the treatment of autoimmune diseases. (nature.com)
  • McGeachy, M. J. & Cua, D. J. Th17 cell differentiation: The long and winding road. (nature.com)
  • The orphan nuclear receptor RORγt directs the differentiation program of proinflammatory IL-17 + T helper cells. (nature.com)
  • Manel, N., Unutmaz, D. & Littman, D. R. The differentiation of human TH-17 cells requires transforming growth factor-β and induction of the nuclear receptor RORγt. (nature.com)
  • Zhang, F. P., Meng, G. X. & Strober, W. Interactions among the transcription factors Runx1, RORγt and Foxp3 regulate the differentiation of interleukin 17-producing T cells. (nature.com)
  • Akiba H, Miyahira Y, Atsuta M. Critical contribution of OX40 ligand to T helper cell type 2 differentiation in experimental leishmaniasis. (bdbiosciences.com)
  • RNA sequencing revealed the upregulation of inflammasome pathway-related genes, keratinocyte stress marker genes, and keratinocyte differentiation marker genes in the Nlrp1b KI mice after UVB irradiation. (frontiersin.org)
  • Interdependence of cortical thymic epithelial cell differentiation and T-lineage commitment. (southernbiotech.com)
  • However, murine models, including knockout and transgenic mice, have been useful in understanding the mechanisms of B lymphopoiesis, B-cell differentiation, and antibody formation. (medscape.com)
  • FGF-10 immunostaining could be related to cell proliferation in epithelium and cell differentiation in epithelium and ectomesenchyme. (bvsalud.org)
  • OGG1 and SMAD7 interact to induce fibroblast proliferation and differentiation and display roles in fibrotic murine and IPF patient lung tissue. (lu.se)
  • Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)
  • In addition, tumor models were characterized in vivo in C57Bl/6NCrl mice in terms of their histological properties, tumor growth kinetics, and radiosensitivity. (biomedcentral.com)
  • The experiment saw the activation of CD8+T cells in C57BL/6 mice, leading to the development of hemorrhagic demyelination within 24 hours. (news-medical.net)
  • C57BL/6 mouse cytotoxic T lymphocyte cell line CTLL-2 was stained with Rat Anti-Mouse CD25-APC (SB Cat. (southernbiotech.com)
  • 2022. Comparison of gelatin flavors for oral dosing of C57BL/6J and FVB/N mice. (awionline.org)
  • C57BL/6 mice were intranasally exposed to swine confinement facility dust extract (DE) or saline daily for 1 wk or allowed to recover for 3-7 days while being treated with an AREG-neutralizing antibody or recombinant AREG. (cdc.gov)
  • Methods: Bone marrow-derived DC from C57BL/6 mice were loaded with UV-irradiated, syngeneic Panc02 carcinoma cells and were administered subcutaneously. (uni-muenchen.de)
  • We show that mouse uterine ILC2s have a heightened type-2 gene signature and expand during pregnancy. (elifesciences.org)
  • Absence of ILC2s leads to utero-placental abnormalities, including poor vascular remodelling, increased Il1b and decreased Il4, Il5 , and Il13 gene expression, and reduced alternative activation of dendritic cells (DCs) and macrophages. (elifesciences.org)
  • Compared with N-803+αPD-L1, N-809 enhanced natural killer (NK) and CD8 + T-cell activation and function in the dLN and TME, relating to increased gene expression associated with interferon and cytokine signaling, lymphoid compartment, costimulation and cytotoxicity. (bmj.com)
  • Transcriptome comparisons of SOX9+ cells with GLT1+ cells showed that the two populations of cells exhibit largely overlapping gene expression. (jneurosci.org)
  • SR1001 inhibited the development of murine T H 17 cells, as demonstrated by inhibition of interleukin-17A gene expression and protein production. (nature.com)
  • Our main tool for studying neuroinflammation is the SOD1 G93A transgenic mouse, which carries the human gene mutation responsible for hereditary amyotrophic lateral sclerosis (ALS, or Lou Gehrig's disease). (utoledo.edu)
  • Brca1 expression is regulated by a bidirectional promoter that is shared by the Nbr1 gene in mouse. (nih.gov)
  • Isolation of the murine Nbr1 gene adjacent to the murine Brca1 gene. (nih.gov)
  • Murine gene mutations in human counterparts may be associated with a clinical illness different from the illness seen in mice. (medscape.com)
  • The small molecule OGG1 inhibitor, TH5487, decreases myofibroblast transition and associated pro-fibrotic gene expressions in fibroblast cells. (lu.se)
  • Sorl1 gene knockout in mice accelerated the breakdown of triacylglycerides in adipocytes and protected the animals from diet-induced obesity. (medscape.com)
  • immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)
  • During serial passage of mouse embryo fibroblasts in vitro it was found that an established line of cells was more susceptible to murine sarcoma virus transformation than early passages of the cells from which it was derived. (microbiologyresearch.org)
  • Characteristics of the in vitro assay of murine sarcoma virus (moloney) and virus-infected cells. (microbiologyresearch.org)
  • Morphological transformation of mouse embryo cells in vitro by murine sarcoma virus (harvey). (microbiologyresearch.org)
  • Therefore, the aim of our study was to develop a transplantable immunocompetent mouse model of HPV-positive OSCC and characterize it in vitro and in vivo. (biomedcentral.com)
  • After confirming stable expression of HPV-16 E6 and E7 with quantitative real-time PCR and immunofluorescence staining, the cell lines were further characterized in vitro using proliferation assay, wound healing assay, clonogenic assay and RNA sequencing. (biomedcentral.com)
  • Characterization of the resulting MOC1-HPV cell lines and tumor models confirmed stable expression of HPV-16 oncogenes and differences in cell morphology, in vitro migration capacity, and tumor microenvironment characteristics. (biomedcentral.com)
  • It has been reported that FR70 antibody blocks binding of mCD27-Ig fusion protein to mouse Cd70-transfected cells and inhibits some in vitro T-cell- and NK-cell-dependent responses. (bdbiosciences.com)
  • Monoclonal anti-IL 2 receptor antibodies as specific inhibitors of T cell function in vitro. (southernbiotech.com)
  • Additional reported applications (for the relevant formats) include: immunoprecipitation 1 , in vitro and in vivo modulation of B cell responses 2-4 , immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections 5,6 , and spatial biology (IBEX) 14,15 . (biolegend.com)
  • The objective of this study was to determine the role of AREG in altering recovery from environmental dust exposure in a murine in vivo model and in vitro using cultured human and murine lung fibroblasts. (cdc.gov)
  • hOX40 is functional and hOX40L induces increased T-cell proliferation in vitro . (genoway.com)
  • In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)
  • In the pre-clinical murine model, administering anti-CSF-1R antibody prior to and after GVAX/αPD-1 ("pre/post-αCSF-1R + αPD-1 + GVAX") enhanced the survival rate compared to GVAX/αPD-1 dual therapy ( p = 0.005), but administering anti-CSF-1R only before GVAX/αPD-1 did not ( p = 0.41). (bmj.com)
  • NK cells are specialized effectors of the innate immune system that destroy their targets by antibody-dependent cell-mediated cytotoxicity, have prominent antitumor effects, and are potent killers of virally infected cells. (medscape.com)
  • The FR70 monoclonal antibody specifically binds to mouse CD70, a 30-33-kDa type-II transmembrane glycoprotein belonging to the TNF/NGF superfamily. (bdbiosciences.com)
  • However, study on the effects of IL-27/anti-IL-27 antibody (anti-IL-27p28-Ab) administration to atherosclerosis model in ApoE -/- mice has not been reported. (hindawi.com)
  • APC Anti-Mouse CD25 antibody for use in flow cytometry assays. (southernbiotech.com)
  • CD4 + T cells stimulate B cells to induce significant level of antimalarial antibody response and also help in the induction of CD8 + T-cell responses which help in arresting the growth of liver-stage parasites in the host. (omicsonline.org)
  • Treatment with the anti-AREG antibody prevented resolution of DE exposure-induced airway influx of total cells, neutrophils, and macrophages and increased levels of TNF-a, IL-6, and CXCL1. (cdc.gov)
  • Surface associated glycoproteins of endothelial origin, or derived from pericytes, intravascular leukocytes, and plasma, are other important components of the glycocalyx, constituting a vascular cell surface proteome that is dynamic, tissue-specific, and sensitive to changes in vascular homeostasis, blood infection, and inflammation. (lu.se)
  • Littman, D. R. & Rudensky, A. Y. Th17 and regulatory T cells in mediating and restraining inflammation. (nature.com)
  • Binding of complement to a foreign substance, or antigen, amplifies and augments the body's innate immune system by means of its role as an opsonin (a factor that enhances phagocytosis of unwanted particles) and as a chemoattractant (a factor that recruits cells to areas of inflammation). (medscape.com)
  • We demonstrated that UVB irradiation induces IL-1β upregulation and IL-1β-dependent inflammation via caspase-1 activation in these Nlrp1b KI mice. (frontiersin.org)
  • The skin inflammation and hyperkeratosis from UVB irradiation in the Nlrp1b KI mice were inhibited by both intraperitoneal and subcutaneous administration of anti-IL-1β antibodies before UVB irradiation. (frontiersin.org)
  • Atherosclerosis, which is characterized by chronic inflammation in the arterial wall, is driven by immune cells and cytokines. (hindawi.com)
  • Many studies have indicated that numerous immune cells promote chronic inflammation and progressive plaque growth [ 4 ]. (hindawi.com)
  • Thus, development of strategies targeting particular subsets of inflammatory cells and providing well-timed resolution of inflammation may be crucial for proper healing and recovery. (springer.com)
  • Meanwhile IL-27 exerts proinflammatory effects by promoting Th1, CD8, natural killer (NK), T follicular helper (Tfh), and B cell proliferation/functions and by inhibiting Treg and Th2 cell generation in parasites infection [ 4 ]. (hindawi.com)
  • hCD28 is functional: α-human CD28 co-stimulation induces T cell activation and proliferation. (genoway.com)
  • In response to mito- pendence between the key mecha- gens, cell proliferation is triggered by nistic characteristics. (who.int)
  • H - Ras cell proliferation are also linked with haematopoietic cel s. (who.int)
  • Our results reveal substantial differences between murine and human whole-blood models infected with C. albicans and thereby demonstrate limitations in the translatability of this ex vivo model between hosts. (frontiersin.org)
  • In mice, as the most commonly used laboratory animal for in vivo experiments, the blood volume that can be withdrawn repeatedly is very limited and thereby hampers in depth analysis of interactions within blood. (frontiersin.org)
  • Therefore, we previously established an ex vivo human whole-blood infection model that allowed us to define which immune cells interact with the human fungal pathogen Candida albicans ( 4 ), to identify cross-talk between different components of the host response ( 5 ), and to detect substantial differences between related fungal pathogens ( 6 ). (frontiersin.org)
  • Expression of cell-cycle regulator CDK2-associating protein 1 (p12CDK2AP1) in transgenic mice induces testicular and ovarian atrophy in vivo. (nih.gov)
  • Human tissue-engineered colon forms from postnatal progenitor cells: an in vivo murine model. (ca.gov)
  • These data identify distinct electrophysiologic abnormalities in FHC mice with a specific alpha-myosin mutation, and also validate a novel method to explore in vivo the relationship between specific genotypes and their electrophysiologic phenotypes. (jci.org)
  • Background: Tumor-specific cytotoxic T lymphocytes (CTL) can be activated in vivo by vaccination with dendritic cells (DC). (uni-muenchen.de)
  • hLIGHT is functional and its stimulation increases T-cell activation in vivo . (genoway.com)
  • We further demonstrated that similar to non-lymphoid dendritic cells (DCs), CD103(+) LCs originate from pre-DCs, whereas CD11b(+) LCs differentiate from both pre-DCs and monocytic precursors. (nih.gov)
  • Plasmacytoid dendritic cells (pDCs) are rare central regulators of antiviral immunity and unsurpassed producers of interferon-α (IFN-α). (nih.gov)
  • Plasmacytoid dendritic cells (pDCs) are the professional producers of IFN-I in response to many viruses, including all of the herpesviruses tested. (mdpi.com)
  • 0.05), but not mature dendritic cells ( p = 0.132). (bmj.com)
  • Other cellular components of the innate immune system include mononuclear phagocytes, dendritic cells, and NK cells. (medscape.com)
  • CD70 has been observed to be expressed on dendritic cells of Leishmania major-infected mice. (bdbiosciences.com)
  • Dendritic cell-based vaccination combined with gemcitabine increases survival in a murine pancreatic carcinoma model. (uni-muenchen.de)
  • An explanation can be made in terms of the growth rates of the cells involved, although primary cells were more susceptible to murine sarcoma virus than would be expected from their rate of growth. (microbiologyresearch.org)
  • Non-dividing (amitotic) cells were not susceptible to murine sarcoma virus transformation. (microbiologyresearch.org)
  • Requirements for RNA and DNA synthesis in the transformation of mouse embryo cells by murine sarcoma virus (moloney). (microbiologyresearch.org)
  • Production of altered cell foci in tissue culture by defective moloney sarcoma virus particles. (microbiologyresearch.org)
  • Replication of murine sarcoma virus-HARVEY in tissue cultures of virus-induced sarcomas. (microbiologyresearch.org)
  • Effect of cytosine arabinoside on the replication of the Moloney sarcoma virus in 3T3 cell cultures. (microbiologyresearch.org)
  • Infection of stationary cultures with murine sarcoma virus (harvey). (microbiologyresearch.org)
  • Transformation by murine sarcoma virus: fixation (deoxyribonucleic acid synthesis) and development. (microbiologyresearch.org)
  • Different cell culture characteristics of two strains of murine sarcoma virus. (microbiologyresearch.org)
  • SIGNIFICANCE STATEMENT Astrocytes are traditionally identified immunohistochemically by antibodies that target cell-specific antigens in the cytosol or plasma membrane. (jneurosci.org)
  • I. Monoclonal antibodies that define distinct functional epitopes on activated T cells and react with activated B cells. (southernbiotech.com)
  • T cells provide protection to infection by cytokine-mediated mechanisms or through production of antibodies. (omicsonline.org)
  • Experiments with B cell deficient mice have demonstrated that B cells and antibodies are essential for complete clearance of parasites from blood of the host [2]. (omicsonline.org)
  • In the absence of mature B cells, patients lack lymphoid tissue and fail to develop plasma cells, the cells that manufacture antibodies. (medscape.com)
  • cells are activated by interaction with antigens and obtain T indicated that the cell lines representing a defined cell help, they mature into terminally differentiated plasma stage generally presented a high similarity in over- cells secreting large amounts of antibodies [10 -12]. (lu.se)
  • Pathologic and virologic studies of tumors induced in mice by two strains of murine sarcoma vims. (microbiologyresearch.org)
  • Infection with high-risk human papillomavirus (HPV) strains is one of the risk factors for the development of oral squamous cell carcinoma (OSCC). (biomedcentral.com)
  • The team did a genetic search in the strains of mice previously used for xenografting the prostate tumor cells. (nih.gov)
  • CD25 is expressed on activated T cells, B cells, NK cells, and monocytes of all mouse strains tested. (southernbiotech.com)
  • Although the cell lines did not differ in their intrinsic radiosensitivity, one of the HPV-positive tumor models, MOC1-HPV K1, showed a significantly longer growth delay after irradiation with a single dose of 15 Gy compared to parental MOC1 tumors. (biomedcentral.com)
  • Consistent with this, MOC1-HPV K1 tumors had a lower percentage of hypoxic tumor area and a higher percentage of proliferating cells. (biomedcentral.com)
  • When studying cancer, researchers often graft human tumors onto mice to create what are called xenografts. (nih.gov)
  • CD300lf is found on mast cells of both murine species and humans, but definite proof of its function in human norovirus infections remains unknown. (wikipedia.org)
  • In the laboratory, these viruses can infect cells from other species, including humans. (nih.gov)
  • The previously reported pathogenic mutation in NLRP1 in humans, and the missense mutation we inserted into the BALB/c mice in this study. (frontiersin.org)
  • Mouse models have milder disease than humans. (medscape.com)
  • The lower neutrophil number in murine blood only partially explains insufficient infection and filamentation control, as spiking with murine neutrophils had only limited effects on fungal killing. (frontiersin.org)
  • Neutrophils are the first immune cells that infiltrate the damaged tissue as early as 24 h after injury. (springer.com)
  • Neutrophils and activated macrophages (CD11c+ CD11bhi) persisted after recovery in lung tissues of anti-AREG-treated mice. (cdc.gov)
  • Oshima H, Nakano H, Nohara C. Characterization of murine CD70 by molecular cloning and mAb. (bdbiosciences.com)
  • Additionally, characterization of the tumor microenvironment such as interactions with immune cells remain largely unknown. (springer.com)
  • Collectively, in a tissue-dependent manner, murine LCs differentiate from at least three distinct precursors (embryonic, pre-DC, and monocytic) in steady state. (nih.gov)
  • 1994. Ah receptor in embryonic mouse palate and effects of TCDD on receptor expression. (cdc.gov)
  • In conclusion, we developed and characterized a novel immunocompetent mouse model of HPV-positive OSCC that exhibits increased radiosensitivity and enables studies of immune-based treatment approaches in HPV-positive OSCC. (biomedcentral.com)
  • We adapted the human whole-blood model to murine blood. (frontiersin.org)
  • Flow cytometry analysis of tumor infiltrating T-cells in a murine model of PDAC was performed to investigate the therapeutic effects and mechanism of anti-CSF-1R/anti-PD-1/GVAX combination immunotherapy. (bmj.com)
  • Expression of SOX9 did not decrease during aging and was instead upregulated by reactive astrocytes in a number of settings, including a murine model of amyotrophic lateral sclerosis (SOD1G93A), middle cerebral artery occlusion, and multiple mini-strokes. (jneurosci.org)
  • This experiment demonstrated the first murine model of AHLE. (news-medical.net)
  • Brain region- and age-dependent dysregulation of p62 and NBR1 in a mouse model of Huntington's disease. (nih.gov)
  • 2022. Improving well-being and survival in the 6-OHDA lesion model of Parkinson´s disease in mice: Literature review and step-by-step protocol. (awionline.org)
  • The aim of this study was to determine the role of Hmox1 in the monocyte/macrophage-mediated cardiac remodeling in a mouse model of MI. (springer.com)
  • Here, we used a murine pancreatic carcinoma model to investigate whether combination with gemcitabine increases therapeutic efficacy of DCbased vaccination. (uni-muenchen.de)
  • Moreover, they represent a powerful model system for studying how compounds modulate immune cell response and/or stroma cells in a physiological microenvironment. (genoway.com)
  • The humanized PD-1 mouse model (hPD-1) expresses human PD-1 instead of murine PD-1 while the regulation of PD-1 expression is kept under the mouse promoter. (genoway.com)
  • The humanized STING model, developed by Knockin at the mouse STING locus, expresses a full human STING. (genoway.com)
  • The double-humanized CD3ε/CD28 mouse model (hCD3ε/hCD28) expresses the human epitope of the CD3ε chain and a chimeric CD28 with a human extracellular domain. (genoway.com)
  • This model was generated by intercrossing hCD3ε and hCD28 mice. (genoway.com)
  • This model was generated by intercrossing hPD-1 and hPD-L1 mice. (genoway.com)
  • Human CD22 Inhibits Murine B Cell Receptor Activation in a Human CD22 Transgenic Mouse Model. (bvsalud.org)
  • In addition, TH5487 decreases levels of pro-inflammatory mediators, inflammatory cell infiltration, and lung remodeling in a murine model of bleomycin-induced pulmonary fibrosis conducted in male C57BL6/J mice. (lu.se)
  • Experiments with endothelial cells fortified with the isolated fraction from native honey enriched in antioxidants, exposed to peroxyl radicals from 1,1-diphenyl-2-picrylhydrazyl (AAPH, 10 mM) and to hydrogen peroxide (H2O2, 50-100 microM), indicated that phenolic acids and flavonoids were the main causes of the protective effect. (researchgate.net)
  • We have previously shown that gemcitabine sensitizes human pancreatic carcinoma cells against CTL-mediated lysis. (uni-muenchen.de)
  • Two monoclonal HPV-positive OSCC mouse cell lines were established by inducing the expression of HPV-16 oncogenes E6 and E7 in the MOC1 OSCC cell line using retroviral transduction. (biomedcentral.com)
  • CD28-independent costimulation of T cells by OX40 ligand and CD70 on activated B cells. (bdbiosciences.com)
  • The human OX40 expression mirrors the murine OX40 expression. (genoway.com)
  • Despite often comprising only a small proportion of tissue resident immune cells, ILCs are present in most tissues and have been shown to orchestrate inflammatory responses to viruses, intracellular bacteria and parasites. (elifesciences.org)
  • Single-cell dissection of the obesity-exercise axis in adipose-muscle tissues implies a critical role for mesenchymal stem cells. (broadinstitute.org)
  • Histopathology, in situ hybridization, and western blotting were performed on murine tissues. (molvis.org)
  • Native honey (1% w/v pH 7.4, 10(6) cells) showed strong quenching activity against lipophilic cumoxyl and cumoperoxyl radicals, with significant suppression/prevention of cell damage, complete inhibition of cell membrane oxidation, of intracellular ROS production and recovery of intracellular GSH. (researchgate.net)
  • A multitasking plasma membrane protein coordinates cell division and energy metabolism in healthy-and perhaps also cancerous- Drosophila cells. (the-scientist.com)
  • Finally, the researchers determined that in cultured adipose cells, SORLA acts as a sorting factor for the insulin receptor that redirects internalized receptor molecules from endosomes to the cell surface membrane. (medscape.com)
  • Furthermore, it was reported that DPP8/9 inhibitors activate the murine NLRP1B inflammasome ( 13 - 16 ). (frontiersin.org)
  • Tyrosinase inhibitors specifically interact with melanogenic cells and do not lead to side effects compared with other melanogenesis inhibitors [ 3 ]. (hindawi.com)
  • Neuroinflammation is manifest by activation of microglial cells in response to dysregulated cytokine networks and may be triggered by deposits of toxic protein or dead cell debris. (utoledo.edu)
  • This was accompanied by higher numbers of Ly6C hi monocytes in peripheral blood, as well as higher expression of monocyte chemoattractant protein-1 and adhesion molecules in the hearts of MI-operated Hmox1 −/− mice. (springer.com)
  • Western blot analysis and in situ hybridization confirmed the presence of AND-34 RNA and protein in lens epithelial cells, particularly at the lens equator. (molvis.org)
  • DeMarini, and Chapter 20, by Rice and cell death determine the size protein in several signal ing path- and Herceg). (who.int)
  • 2022. Effects of hair removal creams on mouse skin. (awionline.org)
  • 2022. Refinement of handling and dosing methods for rats and mice. (awionline.org)
  • Innate lymphoid cells (ILCs) lack antigen receptors but are equipped with receptors for cytokines, hormones, and paracrine signals enabling them to sense their environment and respond quickly by producing factors that influence parenchymal cells and other resident immune cells. (elifesciences.org)
  • IL-27 is a member of the IL-12 family and is composed of Epstein-Barr virus-induced gene3 (EBI3) and p28 subunits, which are produced by active antigen-presenting cells [ 1 ]. (hindawi.com)
  • Natural-killer (NK) cells are specialized lymphocytes that have cytotoxic properties in addition to their ability to produce cytokines that assist in the orchestration of adaptive immunity. (medscape.com)
  • The adaptive immune system consists of 2 types of lymphocytes: T cells (70-75% of the adaptive immune force) and B cells (10-20% of the adaptive immune force). (medscape.com)
  • IL-27 binds to IL-27ra and gp-130 complex, which is expressed on multiple cell types, including T lymphocytes [ 2 ]. (hindawi.com)
  • Murine Norovirus Infection Induces T1 Inflammatory Responses to Dietary Antigens. (broadinstitute.org)
  • The generation of receptors specific for antigens is a unique and complex process that generates 10 12 specific receptors for each cell type of the adaptive immune system, including T and B cells. (medscape.com)
  • Various cell types can process and present these antigens to T cells, or antigens may be soluble and bound to B-cell receptors. (medscape.com)
  • In CF tests hyperimmune sera or ascitic fluids prepared in mice against Sunday Canyon virus did not react with any of 139 virus antigens of diverse origin. (cdc.gov)
  • In other CF tests, potent Sunday Canyon virus antigens failed to fix complement in the presence of hyperimmune fluids prepared against 177 viruses or of 6 pools of sera randomly selected at various times from the Rocky Mountain Laboratory (RML) normal mouse colony. (cdc.gov)
  • In mice, overexpression of human SORLA in murine adipose tissue blocked hydrolysis of triacylglycerides and caused excessive adiposity. (medscape.com)
  • Development of 3T3-like lines from Balb/c mouse embryo cultures: transformation susceptibility to SV40. (microbiologyresearch.org)
  • Kumar V, Rakha A, Saroa R, Bagai U (2015) CD4+T Cells Expansion in P. berghei (NK-65) Infected and Immunized BALB/C Mice. (omicsonline.org)
  • Recent studies demonstrate that similar epigenetic clocks for mice (Mus Musculus) can be slowed by gold standard anti-aging interventions such as calorie restriction and growth hormone receptor knock-outs. (sens.org)
  • Foremost, N-809 enhances intratumoral lymphocyte numbers by increasing trafficking via altered chemokine levels in the TME and chemokine receptor expression on CD8 + T cells and NK cells. (bmj.com)
  • The murine IL 2 receptor. (southernbiotech.com)
  • This enhances insulin receptor surface expression and strengthens insulin signal reception in the cells. (medscape.com)
  • However, since HPV can only infect human cells, there are only a few immunocompetent mouse models available that enable immunological studies. (biomedcentral.com)
  • Plant Cell Wall Proteins: A Large Body of Data, but What about Runaways? (mdpi.com)
  • To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)
  • Murine means related to rats and mice. (msdmanuals.com)
  • Thus, the rickettsiae that cause murine typhus live mainly in rats and mice (the hosts). (msdmanuals.com)
  • We also observed host-dependent differences for interaction of platelets with C. albicans , showing enhanced platelet aggregation, adhesion and activation in murine blood. (frontiersin.org)
  • Defective respiration and one-carbon metabolism contribute to impaired naïve T cell activation in aged mice. (broadinstitute.org)
  • Cell-to-cell interactions set off a cascade of events that may result in T- or B-cell activation and, ultimately, host defense. (medscape.com)
  • Activation of Btk results in a cascade of signaling events resulting in calcium mobilization and fluxes, cytoskeletal rearrangements, and transcriptional regulation involving nuclear factor-kappaB (NF-kappaB) and nuclear factor of activated T cells (NFAT). (medscape.com)
  • This inhibition was cell contact-dependent and soluble factor-independent, as indicated by trans-well experiments. (nih.gov)
  • reported that DPP9 inhibition causes NLRP1-dependent ASC speck formation and the cleavage of IL-1β in human cells ( 17 ). (frontiersin.org)
  • Increased TME NK and CD8 + T-cell numbers correlated with augmented chemokine ligands and receptors. (bmj.com)
  • Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibiting mouse cells. (microbiologyresearch.org)
  • Thus, N-809 can potentially stimulate effector immune cells through IL-15 and block immunosuppressive PD-L1. (bmj.com)
  • Group 2 innate lymphoid cells (ILC2s) adapt to tissue physiology and contribute to immunity, inflammatory pathology and metabolism. (elifesciences.org)
  • This important study describes the protective role of group 2 innate lymphoid cells (ILC2s) in tissue physiology and contributes to immunity, inflammatory pathology, and metabolism in maintaining homeostasis during pregnancy. (elifesciences.org)
  • Both heterogeneity and tissue specialization of immune cells may hold the key to understanding the pathophysiology of some important pregnancy complications, such as miscarriage and fetal growth restriction (FGR), which is defined as the failure of the fetus to achieve its genetically determined growth potential. (elifesciences.org)
  • A multi-tissue full lifespan epigenetic clock for mice. (sens.org)
  • Dissociating cells from specific tissue types requires specific parameters for tissue agitation to obtain a high volume of viable, culturable cells. (jove.com)
  • A small percentage of AND-34 −/− mice show distinctive small white eye lesions resulting from the migration of ruptured cortical lens tissue into the anterior chamber. (molvis.org)
  • Germinal centers where B cells proliferate and differentiate are poorly developed in all lymphoid tissue, including the spleen. (medscape.com)
  • Here, we describe an experimental protocol to chemically tag and quantify the vascular cell surface proteome in murine models of bacteremia, in a time-resolved and. (lu.se)
  • Meanwhile, the number of T helper 1 (Th1) and T helper 17 (Th17) cells was decreased. (hindawi.com)
  • Importantly, recent studies have shown that the importance of type 17 T helper cells (Th17 cells) and regulatory T cells (Tregs) in the pathogenesis of various immune disorders, particularly in atherosclerosis. (hindawi.com)
  • In Plasmodium chabaudi chabaudi (AS), T helper cells of host have been reported to be necessary for inducing a protective immune response against the blood stages of parasite [1]. (omicsonline.org)
  • Depending upon the cytokines secreted by the Helper T cells, they are classified into two subpopulations: Th1 and Th2 cells. (omicsonline.org)
  • We found that SOX9 is almost exclusively expressed by astrocytes in the adult brain except for ependymal cells and in the neurogenic regions, where SOX9 is also expressed by neural progenitor cells. (jneurosci.org)
  • Conclusion: Colon can form from human progenitor cells on a scaffold in a mouse host. (ca.gov)
  • B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)
  • Murine norovirus (MNV) is a species of norovirus affecting mice. (wikipedia.org)
  • Institute for Cell Biology and Neuroscience, Department Molecular and Cellular Neurobiology, Max von Laue Str. (mdpi.com)
  • NLRP1 senses pathogen-associated molecular patterns (PAMPs) mainly derived from infection, and danger-associated molecular patterns (DAMPs) derived from damaged or dying cells ( 5 ). (frontiersin.org)
  • A molecular signature makes it possible to trace the details of hair cell replacement in the mammalian inner ear. (the-scientist.com)
  • Uterine ILC2s are key immune cells during normal and complicated pregnancies. (elifesciences.org)
  • Furthermore, immunofluorescence staining of blood vessels, hypoxic areas, proliferating cells and immune cells was performed to characterize the tumor microenvironment of all three tumor models. (biomedcentral.com)
  • In addition, N-809 reduces immunosuppressive and pro-tumorigenic immune cells in the TME, including T reg , M2-like macrophages and M-MDSC. (bmj.com)
  • However, our understanding of how pathogens interact with cellular and humoral host factors in blood is limited, mainly due to technical issues: While it is relatively easy to study the interaction of pathogens with isolated blood cells, or their survival in serum or plasma, such approaches lack the complexity of interactions between different types of immune cells and additional factors, e.g., complement, present in blood. (frontiersin.org)
  • Immunofluorescence staining confirmed human origin, identified differentiated epithelial cell types and verified the presence of supporting mesenchyme. (ca.gov)
  • Expression of AND-34 regulates epithelial cell growth pattern, motility, and growth factor dependence. (molvis.org)
  • The human VISTA expression pattern in homozygous mice is similar to murine VISTA in wild-type mice. (genoway.com)
  • Murine infection models are widely used to study systemic candidiasis caused by C. albicans . (frontiersin.org)
  • Mice that live in a more-stimulating environment for two weeks appear to develop a more-inflammatory immune state that might help protect the animals against infection. (the-scientist.com)
  • CD4 + CD25 + FoxP3 + regulatory T cells (Tregs) are also involved in the mechanism of immune regulation against Plasmodium infection. (omicsonline.org)
  • Natural Treg cells during primary exposure to malaria enhanced Th1 memory responses and increased disease severity during re-infection. (omicsonline.org)
  • The infection is transmitted to people by rat, mouse, and cat fleas. (msdmanuals.com)
  • While AND-34 is widely expressed in wild type mice, histologic analysis of multiple organs in AND-34 −/− mice is unremarkable and analyses of lymphocyte development show no overt changes. (molvis.org)
  • Unlike human blood, murine blood was unable to reduce fungal burden and more substantial filamentation of C. albicans was observed. (frontiersin.org)
  • The xenotropic murine leukemia virus-related virus (XMRV) was first found in samples from a human prostate tumor in 2006. (nih.gov)
  • The virus appears to have infected the human tumor cells while they were in mice. (nih.gov)
  • The scientists postulate that genetic recombination between these 2 viruses generated XMRV while human prostate tumor cells were being grown in a mouse. (nih.gov)
  • The recombined virus then infected the human tumor cells. (nih.gov)
  • To identify a nuclear marker pathognomonic of astrocytic phenotype, we assessed differential RNA expression by FACS-purified adult astrocytes and, on that basis, evaluated the expression of the transcription factor SOX9 in both mouse and human brain. (jneurosci.org)
  • Furthermore, SR1001 inhibited the expression of cytokines when added to differentiated murine or human T H 17 cells. (nature.com)
  • In previous work, human fetal intestinal cells developed epithelium without mesenchyme following implantation in mice. (ca.gov)
  • Materials & methods: Organoid units were prepared from human colon waste specimens, loaded onto biodegradable scaffolds and implanted into immunocompromised mice. (ca.gov)
  • Immunofluorescence staining demonstrated human origin and the three differentiated cell types of mature colon epithelium. (ca.gov)
  • We generated Nlrp1b (the mouse homologue of human NLRP1 ) gain-of-function knock-in ( Nlrp1b KI) mice with UVB irradiation-induced autoinflammatory skin lesions. (frontiersin.org)
  • Figure 1 Homology in domain structures and amino acid sequences between human and mouse NLRP1 and the present knock-in mutation. (frontiersin.org)
  • (A) Domain structures of human NLRP1 and mouse NLRP1B. (frontiersin.org)
  • (B) Similar and conserved amino acid sequences between human NLRP1 and mouse NLRP1B. (frontiersin.org)
  • Human hair removal brands such as Nair™ are sometimes used in research to remove hair from mice before surgery, imaging, and other procedures.1,3 While the use of these depilatory creams in rodent research is relatively. (awionline.org)
  • To determine the safety of the fermented extracts, their cytotoxicity was analyzed by measuring the cell viability of CCD-966SK and human epidermal melanocytes (HEMn) after exposure. (hindawi.com)
  • In murine and human lung fibroblasts, DE induced the release of AREG and inflammatory cytokines. (cdc.gov)
  • The human CTLA-4 expression recapitulates the mouse CTLA-4 expression on T cells. (genoway.com)
  • The human CD28 expression recapitulates murine CD28 expression. (genoway.com)
  • The human CD39 expression recapitulates murine CD39 expression. (genoway.com)
  • Animal models of human BTK mutations are confined to mice at this time. (medscape.com)
  • Key mesenchymal components (smooth muscle, intestinal subepithelial myofibroblasts and ganglion cells) were seen. (ca.gov)
  • A hormone thought to make murine insulin-secreting cells proliferate in mice did not perform in replication studies. (the-scientist.com)
  • Oral squamous cell carcinoma (OSCC) includes malignant neoplasms of the lip, oral cavity and oropharynx. (biomedcentral.com)
  • Background Anti(α)-programmed cell death-1 (PD-1)/programmed death-ligand 1 (PD-L1) monotherapy fails to provide durable clinical benefit for most patients with carcinoma. (bmj.com)
  • Antitumor efficacy of N-809 or N-803+αPD-L1 was evaluated in two murine carcinoma models and an extensive analysis of immune correlates was performed in the tumor and tumor-draining lymph node (dLN). (bmj.com)
  • Hartwig UF, Karlsson L, Peterson PA, Webb SR. CD40 and IL-4 regulate murine CD27L expression. (bdbiosciences.com)
  • Following verification of a stem-like signature (upregulated expression of stemness markers, resistance to chemotherapy, increased spheroid formation, and tumorigenesis capabilities despite inoculation of a small number of cells), we analyzed the interaction of these cells with macrophages via direct and indirect coculture assays. (springer.com)
  • Its expression is initiated by functional rearrangement and expression of IgM heavy chain genes and is down-regulated when immature B cells mature and express IgD. (southernbiotech.com)
  • A viral interference test for mouse leukemia viruses. (microbiologyresearch.org)
  • Closely related murine leukemia viruses are known to cause cancers and other diseases in mice. (nih.gov)
  • The recombination - a common outcome when cells are infected by 2 or more viruses - likely occurred sometime between 1993 and 1996. (nih.gov)
  • The research team found evidence of sequences from the 2 mouse viruses in commercial laboratory reagents. (nih.gov)
  • In Vero cells, Sunday Canyon plaques were not reduced by prior incubation of the virus with potent antisera prepared against Hughes, Punta Salinas, Sapphire II, Sixgun City and Yaquina Head viruses. (cdc.gov)
  • Afterwards, however, the hearts of Hmox1-deficient mice undergo adverse late LV remodeling due to overactive and prolonged post-ischemic inflammatory response. (springer.com)
  • Moreover, in contrast to N-803+αPD-L1, N-809 reduced immunosuppressive regulatory T cells (T reg ), monocytic myeloid-derived suppressor cells (M-MDSC) and M2-like macrophages in the TME. (bmj.com)
  • Splenectomy decreased the numbers of circulating inflammatory Ly6C hi monocytes in blood, reduced the numbers of proinflammatory cardiac macrophages and significantly improved the post-MI LV function in Hmox1 −/− mice. (springer.com)
  • Overview of Rickettsial Infections Rickettsial infections and related infections (such as anaplasmosis, ehrlichiosis, and Q fever) are caused by an unusual type of bacteria that can live only inside the cells of another organism. (msdmanuals.com)
  • The higher number of TME CD8 + T cells was attributed to enhanced infiltration, not in situ expansion. (bmj.com)
  • Resistance to GVAX was associated with an immune-suppressive myeloid cell infiltration, which may limit further therapeutic gains of GVAX/αPD-1 therapy. (bmj.com)
  • Langerhans cells (LCs) populate the mucosal epithelium, a major entry portal for pathogens, yet their ontogeny remains unclear. (nih.gov)
  • It is also expressed on cultured epidermal Langerhans cells. (southernbiotech.com)
  • AREG intranasal treatment for 3 days during recovery phase reduced repetitive DE-induced airway inflammatory cell influx and cytokine release. (cdc.gov)
  • Amphiregulin modulates murine lung recovery and fibroblast function following exposure to agricultur e organic dust. (cdc.gov)
  • Standardized cell cultures are used in MNV propagation and the virus naturally infects mice, which allows studies in animal systems. (wikipedia.org)
  • Recent studies suggested that strategies to reduce immunosuppressive cells, promote systemic T-cell responses and lymphocyte trafficking to the tumor microenvironment (TME) may improve efficacy. (bmj.com)
  • It has been reported that IL-27 performs an anti-inflammatory function by acting on conventional CD4 + T cells to induce IL-10-producing cells that are implicated in controlling inflammatory responses [ 3 ]. (hindawi.com)
  • This subset of T cells play critical role in maintaining immune homeostasis and controlling excessive immune responses [ 5 ]. (omicsonline.org)
  • CD25 + regulatory T cell depletion augments immunotherapy of micrometastases by an IL-21-secreting cellular vaccine. (southernbiotech.com)
  • genOway has developed a catalog of reliable and efficient translational mouse models for immunotherapy expressing humanized immune checkpoints. (genoway.com)
  • The ICP mouse models are co-validated by our partners of the precompetitive consortium, including BMS, Charles River, Alligator and more company leaders in immuno-oncology and immunotherapy. (genoway.com)
  • Activating mutations upstream may also underlie some epigenetic or within the ERK1/2 cascade are events that change cell signalling. (who.int)
  • The biochemical features of murine CD25 have been characterized in detail. (southernbiotech.com)