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  • Spleen
  • Histological analysis of the spleen demonstrated a clear difference in the microarchitectural structure between B6.WT and B6.TNF−/− mice ( Fig. 3a and Fig. b), as described previously 16 17. (nih.gov)
  • Macrophages
  • BALB/c-Apoe(-/-) mice bearing a null mutation in the Npc1 gene display prominent medial erosion and atherothrombosis, whereas their macrophages accumulate free cholesterol in late endosomes and show increased cathepsin K (Ctsk) expression. (umassmed.edu)
  • Culture of mouse peritoneal macrophages with mouse serum induces lipid bodies that associate with the parasitophorous vacuole and decrease their microbicidal capacity against Toxoplasma gondii. (nih.gov)
  • Here, we investigated the role of host LBs in T. gondii infection in mouse peritoneal macrophages in vitro. (nih.gov)
  • Macrophages cultured with mouse serum (MS) had higher numbers of LBs than those cultured in foetal bovine serum and can function as a model to study the role of LBs during intracellular pathogen infection. (nih.gov)
  • macrophages cultured with mouse serum (MS) have a different morphologyappearance, higher number of vesicle-like organelles and greater area.Macrophages cultured with foetal bovine serum (FBS) (A, C) or MS (B, D).Differential interference contrast microscopy (A, B), Bar = 40 μm. (nih.gov)
  • Male
  • Methods and Materials: The colorectum of male C3Hf/Kam (C3H) and C57B1/6 (B6) mice were irradiated using a dose/length combination (30 Gy to 13.7 mm) that resulted in 10 or 100% incidence of obstruction by 6 months in each strain, respectively. (mskcc.org)
  • fluorescence
  • We now show increased cathepsin K immunostaining and increased cysteinyl proteinase activity using near infrared fluorescence imaging over proximal aortas of Apoe(-/-), Npc1(-/-) mice. (umassmed.edu)
  • strain
  • Mice with such a deficiency develop a profound lymphadenopathy, splenomegaly, hypergammaglobulinemia, and strain-dependent systemic autoimmune disease, and succumb to premature death. (nih.gov)
  • To confirm these data in a strain highly permissive for the phenotype, C3H/HeJ.gld and C3H.HeJ.lpr mice were treated with a TNF-specific monoclonal antibody. (nih.gov)
  • A strain is inbred when it has undergone at least 20 generations of brother x sister or offspring x parent mating, at which point at least 98.6% of the loci in an individual of the strain will be homozygous, and each individual can be treated effectively as clones. (wikipedia.org)
  • One type of inbred strain that either has been altered, or naturally mutated so that it is different at a single locus. (wikipedia.org)
  • Setd2
  • These studies reveal four distinct layers of histone modification across inducible mammalian genes and show that HYPB/Setd2 is responsible for H3K36 trimethylation throughout the mouse nucleus. (nih.gov)
  • E) C3H 10T½ cells were untransfected (−) or transfected with Setd2, non-targeting (non-t) or NSD1 siRNA. (nih.gov)
  • splenic
  • There was a significant effect of stimulation by Con A on the percentage of splenic lymphocytes positive for Thy1.2, Lyt-2, L3T4, and goat anti-mouse Ig, regardless of treatment condition. (biomedsearch.com)
  • There was a significantly greater increase in the percentage of Thy1.2+ and, especially of, Lyt-2+ cells in stimulated splenic lymphocytes from untrained mice given an exhaustive exercise session compared with controls and with trained mice. (biomedsearch.com)
  • 40 wk), defined splenic structures in B6.gld mice diminished ( Fig. 3 E), whereas demarcated but small and rim-like B cell areas were still observed in B6.gld.TNF−/− mice ( Fig. 3 G). (nih.gov)
  • either
  • To evaluate whether IGFBP-5 can stimulate osteoblast activity and enhance bone accretion in a mouse model of osteoblast insufficiency, daily subcutaneous injections of either intact [IGFBP-5 (intact)] or carboxy-truncated IGFBP-5 [IGFBP-5-(1--169)] were given to ovariectomized (OVX) mice for 8 wk. (biomedsearch.com)
  • Caveolin could not be detected in microsomal membranes from either mouse cerebellum or SH-SY5Y cells. (lancs.ac.uk)
  • Fetal/neonatal mice, derived from matings of either +/csfmop females with csfmop/csfmop males or the reciprocal pairings, were genotyped and tissue Mphi identified and quantified. (biomedsearch.com)
  • intravenously
  • To establish transplacental transfer of maternal CSF-1, gestational day-17 mothers were injected intravenously with 125I-mouse CSF-1 or human rCSF-1, and the 125I-cpm or human CSF-1 concentrations were measured in fetal tissue, placenta, and fetal/maternal sera. (biomedsearch.com)
  • Cells were isolated by mechanical dissociation of spleens from control mice (0 h) or mice intravenously injected with 25 μg STAg and were cultured for 24 h in medium. (nih.gov)
  • intestinal
  • Expression of genes encoding fat transport and storage proteins in ileal mucosa of mice harboring a simplified human intestinal microbiota (SIHUMI), SIHUMI without C. ramosum (SIHUMIw/oCra), or C. ramosum only (Cra). (nih.gov)
  • membranes
  • In the present study we have isolated detergent-insoluble, caveolae-like membranes from both mouse cerebellum and the human neuroblastoma cell line SH-SY5Y. (lancs.ac.uk)
  • Detergent-insoluble membranes from mouse cerebellum retained nearly all of the glycosylphosphatidylinositol-anchored proteins--alkaline phosphatase, 5'-nucleotidase, and the F3 protein--while excluding the majority of the plasmalemmal marker protein alkaline phosphodiesterase I. Although the inositol trisphosphate receptor was highly enriched in this detergent-insoluble fraction, neither amyloid precursor protein nor clathrin immunoreactivity could be detected. (lancs.ac.uk)
  • cells
  • Here, we demonstrate by immunolocalization experiments and flow cytometric analysis that, contrary to expectation, DC and not MPhi are the initial cells to synthesize IL-12 in the spleens of mice exposed in vivo to an extract of Toxoplasma gondii or to lipopolysaccharide, two well characterized microbial stimulants of the cytokine. (nih.gov)
  • significantly
  • C57B1/6 mice developed colorectal obstruction with significantly higher incidence compared to C3Hf/Kam mice after partial volume irradiation with 30 Gy. (mskcc.org)
  • Ileal gene expression of Cd36 was significantly increased in SIHUMI and Cra mice compared with SIHUMIw/oCra mice (Fig. 5A), while that of Fabp2 only tended to be higher in SIHUMI (P = 0.140) and Cra (P = 0.156) mice than in SIHUMIw/oCra mice (Fig. 5B). (nih.gov)
  • dependent
  • Transcription of Fatp4 and Plin2 was increased in ileal mucosa of SIHUMI mice but not in that of Cra and SIHUMIw/oCra mice (Fig. 5C and D). Given that monoassociation of mice with C. ramosum did not increase Fatp4 and Plin2 expression, we propose that upregulation of these genes by C. ramosum was dependent on other members of the SIHUMI community. (nih.gov)
  • observations
  • At the view of our observations, we screened the mouse IRAK-1 sequence for possible SHP-1 binding sites. (nih.gov)
  • given
  • Three groups of mice were exposed to 8 weeks of treadmill training: one group was sacrificed immediately after training, a second group was sacrificed 72 h after training, and a third group was rested for 72 h and then given an acute exhaustive run. (biomedsearch.com)
  • formation
  • Contrary to the expected outcome, mice deficient for both FasL and TNF had a substantially milder gld phenotype with regard to mortality, lymphoaccumulation, germinal center formation, and hypergammaglobulinemia. (nih.gov)
  • IGF-1/IGFBP-1 increases blastocyst formation and total blastocyst cell number in mouse embryo culture and facilitates the establishment of a stem-cell line. (nih.gov)
  • higher
  • We hypothesized that C. ramosum promoted ileal lipid absorption and thereby contributed to the higher body weight gain and fat deposition in the obese SIHUMI and Cra mice compared with the less obese SIHUMIw/oCra mice. (nih.gov)