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  • vivo
  • Although MDSCs from tumor-bearing WT mice had functional NADPH oxidase, the suppressive effect of MDSCs on ex vivo stimulated T cell proliferation was NADPH oxidase-independent. (jove.com)
  • In all the four tumor models, a single CTX injection resulted in an impressive antitumor response to the subsequent injection of spleen cells from mice immunized with homologous tumor cells independently of the in vivo response to CTX alone. (jci.org)
  • The approach taken was to first generate tumor-specific CTL from mice challenged with the CMS4 sarcoma coadministered with anti-CTLA4 mAb, which has been shown to facilitate the induction of Ag-specific T cell responses in vivo. (jimmunol.org)
  • In vivo, the adoptive transfer of CMS4-reactive CTL (≥1 × 10 6 ) resulted in nearly the complete regression of 3-day established lung metastases. (jimmunol.org)
  • In vivo and/or in vitro approaches could be harnessed to develop CD4 + T cell-based immunotherapy. (frontiersin.org)
  • Protein Ag-loaded DC were used as antigen presenting cells to stimulate T cells in vitro and subsequently analyzed in vivo for their anti-tumor effect via adoptive transfer, a treatment strategy widely studied in clinical trials as a therapy against various malignancies. (springer.com)
  • metastasis
  • Hanna, N., Davis, T. W. , and Fidler, I. J. , 1982, Environmental and genetic factors determine the level of NK activity of nude mice and affect their suitability as models for experimental metastasis. (springer.com)
  • The activity of G3 on mouse mammary tumor cell growth, migration and its effect on spontaneous metastasis to bone in an orthotopic model was modulated by up-regulating the EGFR-mediated signaling pathway. (jove.com)
  • Expression of Foxm1 is elevated in mouse sarcomas that metastasize to the lung and tissue microarray analysis of human MFH correlates overexpression of FOXM1 with metastasis. (jove.com)
  • To understand the mechanism of metastasis, an experimental metastasis assay was established using immunodeficient mice. (jove.com)
  • Experimental metastasis mouse model is a simple and yet physiologically relevant metastasis model. (jove.com)
  • The tumor cells are injected intravenously (i.v) into mouse tail veins and colonize in the lungs, thereby, resembling the last steps of tumor cell spontaneous metastasis: survival in the circulation, extravasation and colonization in the distal organs. (jove.com)
  • Furthermore, the growth of the primary implanted Lewis lung carcinomas and their metastasis were significantly decreased in mice reconstituted with IGF type I receptor-deficient hematopoietic stem cells, indicating that IGF signaling in the hematopoietic tumor stroma supports tumor outgrowth. (jove.com)
  • These studies demonstrate that vitamin D3 treatment increases intratumoral T-cell immune reactivity, and that coupling vitamin D3 treatment to diminish levels of CD34+ NS cells with adoptive immunotherapy enhances the effectiveness of the adoptively transferred tumor-reactive lymph node cells at limiting both metastasis and locoregional tumor recurrence. (ovid.com)
  • antigen
  • HCA587 (also known as MAGE-C2) is a "cancer-testis" antigen highly expressed in a number of malignancies with unique immunological properties, making it a promising target for tumor immunotherapy. (jove.com)
  • was observed in tumor antigen stimulated splenocytes of vaccinated mice. (jove.com)
  • Although gene-modification of T cells to express tumor-related antigen-specific T-cell receptor (TCR) or chimeric antigen receptor (CAR) has clinically proved promise, there still remains room to improve the clinical efficacy of re-directed T-cell based antitumor adoptive therapy. (jove.com)
  • We report here that polyethylene glycol (PEG) linked to near infrared dyes conjugated to chimeric mouse-human anti-carcinoembryonic antigen (CEA) antibody greatly improves imaging of liver metastases in a nude mouse model of colon-cancer experimental metastases. (jove.com)
  • We show that human FVIII specific CAR-Tregs with second generation mouse signaling domains are activated and proliferate in response to antigen recognition, which can cause contact dependent or cytokine dependent suppression of inhibitor producing cells. (bloodjournal.org)
  • We found persistence of adoptively transferred FVIII CAR-Tregs in response to antigen stimulation in mice. (bloodjournal.org)
  • Adoptive cell therapy (ACT) is an emerging approach that necessitates defining robust and efficient methods for the in vitro expansion of antigen-specific T cells then infused into patients. (frontiersin.org)
  • Finally, we discuss the potential interest of these AAPCs, both as fundamental tools to decipher CD4 + T cell responses and as reagents to generate clinical grade antigen-specific CD4 + T cells for immunotherapy. (frontiersin.org)
  • Protein antigen (Ag)-based immunotherapies have the advantage to induce T cells with a potentially broad repertoire of specificities. (springer.com)
  • vaccines
  • As only a minute fraction of peripherally injected vaccines reaches the lymph nodes, vaccinations of mice and humans were performed by direct injection of vaccine into inguinal lymph nodes, i.e. intralymphatic injection. (jove.com)
  • Mice were vaccinated with plasmid DNA, RNA, peptide, protein, particles, and bacteria as well as adjuvants, and strong improvement of immune responses against all type of vaccines was observed. (jove.com)
  • interferons and interleukins), vaccines, and adoptive immunotherapy. (springer.com)
  • Here, we tested in mice several cancer vaccines and an adoptive T cell transfer approach to cancer immunotherapy in combination with several widely used chemotherapeutic drugs. (jci.org)
  • metastases
  • The arisen metastases are isolated and cultured in vitro as cell lines, which often show enhanced metastatic abilities than the parental line when injected again into immunodeficient mice. (jove.com)
  • Tumor-specific CTLs are then injected i.v into the metastases-bearing mouse. (jove.com)
  • Longitudinal MRI was applied to monitor intracranial initiation and development of brain metastases and assess tumor vascular volume and permeability in a mouse model of breast cancer brain metastases. (jove.com)
  • Lymph
  • In mice, a small (5-10 mm) incision is made in the inguinal region of anesthetized animals, the lymph node is localized and immobilized with forceps, and a volume of 10-20 μl of the vaccine is injected under visual control. (jove.com)
  • therapy
  • Here we provide a method for using spinning disk confocal microscopy of live, anesthetized mice to directly observe drug distribution, cancer cell responses and changes in tumor-stroma interactions following administration of systemic therapy in breast cancer models. (jove.com)
  • First, mouse models show that adoptive cell therapy (ACT) can be enhanced by vaccination. (aacrjournals.org)
  • Perhaps the most promising immunotherapy, although not approved by the U.S. Food and Drug Administration, is adoptive cell therapy (ACT). (aacrjournals.org)
  • The suppressive activity of FVIII CAR-Treg on inhibitory antibody formation is now being evaluated in hemophilia A mice that are given factor replacement therapy with FVIII. (bloodjournal.org)
  • high-dose
  • Immunotherapy with high-dose interferon (IFNα2b) is approved in the adjuvant setting and for resected advanced disease, but suffers from significant associated toxicities and only a small portion of patients derive clinical benefits. (aacrjournals.org)
  • treated with recombinant
  • Type I, type III, TNF-α and IL-6 mRNA inducing activity was determined in macrophages from wild type and TLR4 knockout mice treated with recombinant ProTα variants. (plos.org)