• Laminin subunit alpha-5 is a protein that in humans is encoded by the LAMA5 gene. (wikipedia.org)
  • Laminin subunit beta 1 (LAMB1) is involved in attachment, migration, and organization during development, and its elevated expression has been associated with several cancers. (bvsalud.org)
  • ELAV - like1 , methyltransferase like 7A , dolichyl-phosphate mannosyltransferase , laminin subunit beta-1 , gremlin 1 , BCL6 corepressor-like 1 , and three genes of unknown identity. (biomedcentral.com)
  • Altogether, our results demonstrate that βγ subunit of heterotrimeric G proteins play a critical role in neurite outgrowth and differentiation by interacting with MTs and modulating MT rearrangement. (biomedcentral.com)
  • Polyclonal and monoclonal antibodies raised against chicken gizzard 5′-nucleotidase were tested in adhesion assays of embryonic chicken fibroblasts (CEF) for their ability to interfere with the adhesion process of these cells on either laminin or fibronectin substrata. (morulaivf.com)
  • The initial attachment process of CEF on fibronectin and laminin substrata was not influenced by preincubating these cells with antibodies against chicken gizzard 5′-nucleotidase. (morulaivf.com)
  • Previous studies have implicated β1 integrins in cancer since they regulate cell adhesion and migration/invasion on tumor stroma proteins such as fibronectin laminin and collagen (33). (biotech-angels.com)
  • In particular, the laminins, a family of heterotrimeric extracellular glycoproteins, affect tissue development and integrity in such diverse organs as the kidney, lung, skin, and nervous system. (wikipedia.org)
  • Laminin type IV collagen nidogen and sulfated proteoglycans comprise the GBM [37] and laminins are heterotrimeric glycoproteins including one α one β and one γ string. (buyresearchchemicalss.net)
  • The protein encoded by this gene belongs to the alpha subfamily of laminin chains and is a major component of basement membranes. (wikipedia.org)
  • The protein encoded by this gene belongs to the laminin family of secreted molecules. (utsouthwestern.edu)
  • Using our founded cell and knockout/transgenic mouse versions resembling human being NS harboring the R246Q or C321R mutation respectively we've demonstrated that both R246Q and C321R mutations trigger faulty secretion of laminin-521 from podocytes towards the GBM [49 50 Furthermore we've demonstrated how the misfolded C321R mutant protein induces podocyte ER tension and proteinuria [50]. (buyresearchchemicalss.net)
  • ILK forms an obligate heterotrimeric complex with particularly interesting Cys-His-rich protein (PINCH) and parvin, which together lend stability to the actin cytoskeleton at focal adhesions ( Brakebusch and Fässler, 2003 ). (jneurosci.org)
  • Laminin-332 (laminin-5) is a basement membrane heterotrimeric protein composed of alpha-3, beta-3 and gamma-2 laminin chains. (morulaivf.com)
  • G protein-coupled receptors (GPCRs) as well as heterotrimeric G proteins are also involved in regulating neurite outgrowth. (biomedcentral.com)
  • Interleukin-2 receptor (IL-2R) is a heterotrimeric protein expressed on the surface of certain immune cells, such as lymphocytes, that binds and responds to the cytokine IL-2. (transcriptionfactor.org)
  • Analyzing the protein expression patterns of Fas, FADD, caspase 8, CDC25B, fascin, CK14, CK4, annexin I, laminin-5γ2 and SPARC would be valuable to develop rational strategies for early detection of lesions at risk in advance as well as for prevention and treatment of ESCC. (biomedcentral.com)
  • Immunological investigations consisted of direct immunofluorescence (IF), indirect IF with intact and salt-split canine gingival, and salt-split normal or laminin-332-deficient human skin, immunoblotting with purified human laminin-332 and immunoblotting with recombinant NC1 domain of human collagen VII. (morulaivf.com)
  • An embryonic mouse SMG organ culture model was established, and the validity of this model was confirmed. (bvsalud.org)
  • Polyclonal and monoclonal antibodies against chicken gizzard 5 '-nucleotidase inhibit the spreading process of chicken embryonic fibroblasts on laminin substratum. (morulaivf.com)
  • Laminins are heterotrimeric molecules that consist of alpha, beta, and gamma subunits that assemble through a coiled-coil domain. (utsouthwestern.edu)
  • Laminin trimerization happens in the endoplasmic reticulum (ER) and requires association from the three Baricitinib (LY3009104) chains along their laminin coiled-coil domains to create the lengthy arm [39]. (buyresearchchemicalss.net)
  • It is a heterotrimeric molecule composed of alpha1(V), alpha2(V) and alpha3(V) subunits. (lookformedical.com)
  • Human alpha3 heavy chain is 88% aa identical to mouse heavy chain. (bio-techne.com)
  • Upon genuine HDR, we exploited the in vitro adhesion advantage of laminin 332 production to positively select LAMB3-expressing keratinocytes. (unimore.it)
  • Laminins function as heterotrimeric complexes of alpha, beta, and gamma chains, with each chain type representing a different subfamily of proteins. (wikipedia.org)
  • Moreover, integrins, laminins, NF-κB and other regulative molecules were found as crucial proteins. (nature.com)
  • To confirm the immunohistochemical results, three proteins, fascin, CK14 and laminin-5γ2, which were overexpressed in ESCC on tissue microarray, were detected in 12 ESCC cell lines by Western blot assay. (biomedcentral.com)
  • Herein, we report the first application of CRISPR/Cas9-mediated homology direct repair (HDR) to in situ restore LAMB3 expression in JEB keratinocytes in vitro and in immunodeficient mice transplanted with genetically corrected skin equivalents. (unimore.it)
  • In vitro assays demonstrated a competition of laminin and this monoclonal antibody for the binding site on purified 5′-nucleotidase. (morulaivf.com)
  • This study provides evidence for efficient CRISPR/Cas9-mediated in situ restoration of LAMB3 expression, paving the way for ex vivo clinical application of this strategy to laminin 332 deficiency. (unimore.it)
  • Here, through the use of conditional knock-out mice, we demonstrate a requirement for ILK in oligodendrocyte differentiation and axonal myelination in vivo . (jneurosci.org)
  • In metabolic tissues such as the pancreas, adipose, muscle, and liver, laminin isoforms are expressed in discrete temporal and spatial patterns suggesting that certain isoforms may support the development and function of particular metabolic cell types. (bvsalud.org)
  • This review focuses on the research to date detailing the expression of laminin isoforms, their potential function, as well as known pathways involved in laminin signaling in metabolic tissues. (bvsalud.org)
  • We will also discuss the current biomedical therapies involving laminins in these tissues in addition to prospective applications, with the goal being to encourage future investigation of laminins in the context of metabolic disease. (bvsalud.org)
  • Human beta1 extracellular domain is 92%, 94%, 93% and 96% aa identical to the extracellular regions of beta1 in rat, bovine, mouse and feline, respectively. (bio-techne.com)
  • HDR and restored laminin 332 expression were evaluated at single-cell level. (unimore.it)
  • Through interactions with cell surface receptors, laminins stimulate intracellular signaling cascades which direct specific survival and differentiation outcomes. (bvsalud.org)
  • Immunoplates for Exosome capture and quantification from human biofluids (plasma, serum, urine, saliva) and from human or mouse cell media. (transcriptionfactor.org)
  • The names 'acquired junctional epidermolysis bullosa', 'anti-laminin-332 mucous membrane pemphigoid (MMP)' and 'mixed auto-immune subepidermal blistering dermatosis' are proposed for dogs with clinical signs reminiscent of EBA, MMP or BP respectively. (morulaivf.com)
  • PHENOTYPE: Mice homozygous for a targeted null mutation develop a lethal blistering phenotype similar to human junctional epidermolysis bullosa, and die 2-3 days after birth from a failure to thrive. (utsouthwestern.edu)
  • Laminin-332 polypeptides are targeted by auto-antibodies in human patients with mucous membrane (cicatricial) pemphigoid or, more rarely, subepidermal vesicular diseases that resemble epidermolysis bullosa acquisita (EBA) or bullous pemphigoid (BP). (morulaivf.com)
  • In all nine dogs, IgG auto-antibodies detected purified human laminin-332 by immunoblotting. (morulaivf.com)
  • However, the subsequent spreading process of these cells was found to be inhibited for at least 2 h on a laminin substratum. (morulaivf.com)
  • Spreading-arrested and rounded CEF do not develop prominent intracellular stress-fibers like control cells, instead they seem to concentrate their available actin in areas of presumptive initial contact with the laminin substratum. (morulaivf.com)
  • Grafting of genetically corrected skin equivalents onto immunodeficient mice showed a completely restored dermal-epidermal junction. (unimore.it)
  • Once trimers are secreted in to the extracellular space they polymerize to create the supramolecular laminin network via relationships among the NH2-termini from the brief hands (LN domains) [40 41 null mice recapitulate Pierson symptoms [42-47]. (buyresearchchemicalss.net)
  • In particular, the laminins, a family of heterotrimeric extracellular glycoproteins, affect tissue development and integrity in such diverse organs as the kidney, lung, skin, and nervous system. (wikipedia.org)
  • It is thought that laminins mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. (wikipedia.org)
  • The laminins are a family of extracellular matrix glycoproteins that have a heterotrimeric structure consisting of an alpha, beta and gamma chain. (utsouthwestern.edu)
  • Secreted molecules also contribute to the extracellular matrix (fibronectin, laminin, reelin). (frontiersin.org)
  • From NCBI Gene: Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. (nih.gov)
  • Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. (nih.gov)
  • Laminins are alphabetagamma heterotrimeric extracellular proteins that regulate cellular functions by adhesion to integrin and nonintegrin receptors. (ox.ac.uk)
  • Laminins function as heterotrimeric complexes of alpha, beta, and gamma chains, with each chain type representing a different subfamily of proteins. (wikipedia.org)
  • The protein encoded by this gene belongs to the alpha subfamily of laminin chains and is a major component of basement membranes. (wikipedia.org)
  • Time course studies with SM using the mouse ear vesicant model (MEVM) showed progressive histopathologic changes including edema, separation of the epidermis from the dermis, persistent inflammation, upregulation of laminin ýý2 (one of the chains of laminin-332, a heterotrimeric skin glycoprotein required for wound repair), and delayed wound healing from 24h to 168h post exposure. (nih.gov)
  • Laminins are heterotrimeric ECM proteins composed of α, β, and γ chains. (nih.gov)
  • Through combination, laminin chains constitute 15 laminin isoforms. (icosagen.com)
  • Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. (nih.gov)
  • Laminin-332 (laminin-5) is a basement membrane heterotrimeric protein composed of alpha-3, beta-3 and gamma-2 laminin chains. (morulaivf.com)
  • Laminins containing alpha4 and alpha5 chains are expressed in bone marrow, but their interactions with hematopoietic progenitors are unknown. (ox.ac.uk)
  • Integrin alpha6 and beta1 chains were involved in binding of both cell fractions to laminin-10/11 and laminin-8. (ox.ac.uk)
  • We report the crystal structure at 2.13 Å resolution of a minimal integrin-binding fragment of mouse laminin-111, consisting of ∼50 residues of α1β1γ1 coiled coil and the first three laminin G-like (LG) domains of the α1 chain. (nih.gov)
  • Laminin isoforms and their integrin receptors in glioma cell migration and invasiveness: Evidence for a role of alpha5-laminin(s) and alpha3beta1 integrin. (icosagen.com)
  • In functional assays, both laminin-8 and laminin-10/11 facilitated stromal-derived factor-1alpha (SDF-1alpha)-stimulated transmigration of CD34(+) cells, by an integrin alpha6 receptor-mediated mechanism. (ox.ac.uk)
  • Integrins are important laminin receptors, but their binding site on the heterotrimeric laminins is poorly defined structurally. (nih.gov)
  • Laminins are a family of large heterotrimeric proteins that promote cell adhesion and migration via integrins and other cell-surface receptors. (icosagen.com)
  • Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. (nih.gov)
  • Laminins are heterotrimeric molecules that consist of alpha, beta, and gamma subunits that assemble through a coiled-coil domain. (utsouthwestern.edu)
  • An endothelial laminin isoform, laminin-8 (α4β1γ1), is secreted by blood neutrophils, promotes neutrophil migration and extravasation, and protects neutrophils from apoptosis. (icosagen.com)
  • Reactivity of laminin α4 chain specific monoclonal antibody 6C3 on human platelet lysate by Western blotting (reducing, R and nonreducing, NR conditions). (icosagen.com)
  • Polyclonal and monoclonal antibodies against chicken gizzard 5 '-nucleotidase inhibit the spreading process of chicken embryonic fibroblasts on laminin substratum. (morulaivf.com)
  • In vitro assays demonstrated a competition of laminin and this monoclonal antibody for the binding site on purified 5′-nucleotidase. (morulaivf.com)
  • Embryos of transgenic mice in which both alleles of the gamma 1 chain gene were inactivated by homologous recombination, lacked basement membranes, indicating that laminin, gamma 1 chain is necessary for laminin heterotrimer assembly. (nih.gov)
  • Laminins are essential for formation and function of the basement membrane and have additional functions in regulating cell migration and mechanical signal transduction. (utsouthwestern.edu)
  • Laminin isoform-specific promotion of adhesion and migration of human bone marrow progenitor cells. (ox.ac.uk)
  • The cell-adhesive laminins affected migration of hematopoietic progenitors, suggesting a physiologic role for laminins during hematopoiesis. (ox.ac.uk)
  • The functional role of laminin alpha 1 in cerebellum and retinal development suggests that the duplication of LAMA1 may be responsible for at least some of the patient's clinical features. (biomedcentral.com)
  • PHENOTYPE: Mice homozygous for a targeted null mutation develop a lethal blistering phenotype similar to human junctional epidermolysis bullosa, and die 2-3 days after birth from a failure to thrive. (utsouthwestern.edu)
  • PHENOTYPE: Homozygous null mice display embryonic lethality before somite formation with impaired formation of Reichert's membrane. (utsouthwestern.edu)
  • Laminin, alpha 5 has been shown to interact with BCAM. (wikipedia.org)
  • Cooperative PSGL-1 and CXCR2 signaling in neutrophils promotes deep vein thrombosis in mice. (ouhsc.edu)
  • These observations establish laminin-332 as a novel basement membrane antigen in dogs with autoimmune blistering diseases with variable clinical phenotypes. (morulaivf.com)
  • These observations have been validated by C1q gene knock-out mice which showed pre-eclampsia (PE)-like symptoms, characterized by hypertension, proteinuria, glomerular endotheliosis, and increased soluble fms-like tyrosine kinase-1 (sFlt-1)/placental growth factor (PlGF) ratio, and increased oxidative stress. (explorationpub.com)
  • When studied, levels of Laminin-332 autoantibodies correlated with the presence or absence of malignancy. (bvsalud.org)
  • Laminin-γ2 contains an epidermal growth factor (EGF)-like domain in its domain III (DIII or LEb). (nih.gov)
  • OBJECTIVE: To identify several autoimmune diseases that have autoantibodies to Laminin-332 present, and to determine the prevalence of malignancy in them. (bvsalud.org)
  • EVIDENCE REVIEW: A literature search was conducted to identify autoimmune and inflammatory diseases in which autoantibodies to Laminin-332 were present. (bvsalud.org)
  • Using Laminin-332 Pemphigoid (LM-332Pg) as a prototype, to compare clinical profiles of LM-332Pg patients with and without cancer. (bvsalud.org)
  • By identifying the temporal detection of cancer, can the influence of autoantibodies to Laminin-332 on prognosis be determined. (bvsalud.org)
  • Severely damaged mouse ear skin exposed to the vesicant, sulfur mustard (bis-2-chloroethyl sulfide, SM), resulted in increased expression of ER chaperone proteins that accompany misfolded and incorrectly made proteins targeted for degradation. (nih.gov)
  • FINDINGS: Autoantibodies to Laminin-332 were detected in recent studies of systemic lupus erythematosus (SLE), psoriasis, bronchiolitis obliterans (BO), graft-vs-host disease (GVH), bullous pemphigoid (BP), lichen planus (LP), epidermolysis bullosa acquisita (EBA), and membranous glomerulonephropathy (MGN). (bvsalud.org)
  • Sulfur mustard induces an endoplasmic reticulum stress response in the mouse ear vesicant model. (nih.gov)