• Choosing the best therapeutic candidate can be optimized by creating a "humanized mouse" that shows expression of the human antisense transcript in mouse neurons. (cureangelman.org)
  • In perinatal mouse cochlea, we detected a restricted expression of Tbc1d24 in the stereocilia of the hair cells as well as in the spiral ganglion neurons. (nih.gov)
  • To facilitate the study of these neurons, transgenic mice were generated that express enhanced green fluorescent protein (EGFP) in subpopulations of GABAergic neurons. (jneurosci.org)
  • We demonstrate that deletion of ERRγ from DAergic neurons in adult mice was sufficient to cause a levodopa-responsive PD-like phenotype with reductions in mitochondrial gene expression and number, that partial deficiency of ERRγ hastens synuclein-mediated toxicity, and that ERRγ overexpression reduces inclusion load and delays synuclein-mediated cell loss. (nature.com)
  • Altogether, these experiments suggest that ERRγ-deficient mice could provide a model for understanding the regulation of transcription in DAergic neurons and that amplifying ERRγ - mediated transcriptional programs should be considered as a strategy to promote DAergic maintenance in PD. (nature.com)
  • Here, we show that the protein level of ClpP is selectively decreased in αSyn-expressing cell culture and neurons derived from iPS cells of PD patient carrying αSyn A53T mutant, and in dopaminergic (DA) neurons of αSyn A53T mice and PD patient postmortem brains. (springer.com)
  • As humans age, the number of olfactory neurons steadily decreases. (medscape.com)
  • The hypothalamus has emerged as an important area of pathology with selective loss of neurons expressing the neuropeptides orexin (hypocretin), oxytocin and vasopressin in human postmortem HD tissue. (lu.se)
  • Effects of Deletion of Mutant Huntingtin in Steroidogenic Factor 1 Neurons on the Psychiatric and Metabolic Phenotype in the BACHD Mouse Model of Huntington Disease. (lu.se)
  • We modeled these anatomical changes in a transgenic mouse model that overexpresses both P301L tau (uniquely in the medial entorhinal cortex) and mutant APP/PS1 (in a widespread distribution) to examine the anatomical consequences of early tangles, plaques, or the combination. (unboundmedicine.com)
  • We demonstrate that the R6/2 transgenic mouse model expressing a short fragment of mutant HTT displays hypothalamic neuropathology with discrete loss of the neuronal populations expressing orexin, MCH, CART, and orexin at 12 weeks of age. (lu.se)
  • In addition, recurrent tumors expressed genes associated with an epithelial-to-mesenchymal-type transition, and had lost, or at least reduced, expression of mouse homologs of the human RNAs encoding prostate-specific antigens in the ASEL. (genengnews.com)
  • Identification of kidney mesenchymal genes by a combination of microarray analysis and Sall1-GFP knockin mice. (jax.org)
  • We generated triple knock-in (TKI) mice expressing human ACE2, TMPRSS2, and FCGRT in place of murine genes. (aai.org)
  • It brings us one step closer to the overall goal of understanding human brain evolution at the level of genes. (technologyreview.com)
  • Finding these rapidly evolving genes is a starting point to understanding human genetic evolution," says Lahn, "but it doesn't tell us how changes in the genetic sequence lead to changes in human biology. (technologyreview.com)
  • Several genes encoding olfactomedin domain-containing proteins are expressed in the eye. (nih.gov)
  • We focus our attention on the genes that are expressed in RGCs and eye angle tissues. (nih.gov)
  • Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)
  • Fully human monoclonal antibodies are produced using transgenic mice that contain human immunoglobulin genes or using phage display (ie, a bacteriophage-based cloning method) of immunoglobulin genes isolated from human B cells. (msdmanuals.com)
  • Our results indicate that hepatocyte intermediate filament disruption renders mice highly susceptible to hepatotoxicity, and raises the possibility that K18 mutations may predispose to drug hepatotoxicity. (jci.org)
  • Indeed, in colons of mice carrying mutations in Apc (ApcMin/+) or exposed to the carcinogen azoxymethane, elimination of GCC increased tumor initiation by corrupting genomic integrity in the context of disabled compensatory apoptosis. (jefferson.edu)
  • Distinct T cell developmental consequences in humans and mice expressing identical mutations in the DLAARN motif of ZAP-70. (lu.se)
  • This model would assist in finding the appropriate human protein (ATF), or small molecule (ASO), that could turn on the paternal copy of the UBE3A gene, narrowing down candidates that could be used in human clinical trials. (cureangelman.org)
  • In this study, we constructed a recombinant Alphavirus Semliki Forest virus (SFV) replicon vector DREP to express EBOV GP and matrix viral protein (VP40). (frontiersin.org)
  • Placenta expressed transcript 1 is a protein that in humans is encoded by the PLET1 gene. (wikipedia.org)
  • We show, through transmission experiments in transgenic mice expressing porcine prion protein (PrP), that the susceptibility of this mouse model to bovine spongiform encephalopathy (BSE) can be enhanced after its passage in ARQ sheep, indicating that the pathogenicity of the BSE agent is modified after passage in sheep. (cdc.gov)
  • Mice, which are susceptible to SARS-CoV, are resistant to infection with SARS-CoV-2 because of incompatibilities between mouse ACE2 and the viral spike protein. (nature.com)
  • Orthologous to human LRRFIP2 (LRR binding FLII interacting protein 2). (nih.gov)
  • and a second, previously identified and constitutively expressed and non-oxygen-regulated protein known as ARNT. (nobelprize.org)
  • Orthologous to human CSRNP3 (cysteine and serine rich nuclear protein 3). (nih.gov)
  • EGR-1 (Early growth response protein 1) also known as ZNF268 (zinc finger protein 268) or NGFI-A (nerve growth factor-induced protein A) is a protein that in humans is encoded by the EGR1 gene . (wikipedia.org)
  • The human EGR-1 protein contains (in its unprocessed form) 543 amino acids with a molecular weight of 57.5 kDa , and the gene is located on the chromosome 5 . (wikipedia.org)
  • Armed with this information, the researchers decided to block the REST protein in mice. (express.co.uk)
  • The mutant huntingtin protein is ubiquitously expressed, but only certain brain regions are affected. (lu.se)
  • Transmissible spongiform encephalopathies (TSEs) are infectious diseases that affect humans and several livestock species, causing fatal neurodegeneration. (cdc.gov)
  • Smaller terrestrial species commonly express a dual gradient in opsins, with M opsin concentrated superiorly and declining inferiorly, and vice-versa for S opsin. (jneurosci.org)
  • One exception is hu- humanized SCID mice, the use of al oncogenic viruses that are strictly man T-cell lymphotropic virus type 1 surrogate hosts has not proven very species-specific, causing cancer in (HTLV-1): in addition to its ability to useful for defining tumour site con- humans only. (who.int)
  • For this reason, the infect humans, this virus can infect cordance between humans and ex- question about tumour site concor- several other species - including perimental animals. (who.int)
  • For other human tumour virus- primate species are related to the hu- tween data in humans and in experi- es, the use of humanized severe man tumour viruses, the incidence of mental animals is not obvious. (who.int)
  • In this combined immunodeficiency (SCID) cancer is low in these species (as it chapter, some aspects of this issue mice, in which the human target is in humans), which renders cancer are discussed. (who.int)
  • The relieving role of LCN2 inhibition on CNV-induced AMD without toxicity for mice was also demonstrated by in vivo experiments. (hindawi.com)
  • Toxicity studies in mice expressing human hPON1Q192 or hPON1R192 confirm the latter observation. (cdc.gov)
  • In contrast, neuronal populations expressing orexin, oxytocin, vasopressin as well as tyrosine hydroxylase in the A13 area are resistant to QA-induced toxicity. (lu.se)
  • Numerous studies have demonstrated marked differ- fects on terminal bronchioles are seen, but effects in ences in toxicity between rats and mice to styrene ex- the nasal olfactory epithelium do occur, although to posure, especially by inhalation. (cdc.gov)
  • mice developed severe liver toxicity, which probably tio is less than 1, while in mouse liver the ratio is about contributed to the cause of death. (cdc.gov)
  • in an inhalation study and only equivocal evidence of metabolism to SO is below the limit of detection in nearly all samples, and the most active sample of lung lung tumors in two of four chronic toxicity/oncogenicity was approximately 100-fold less active than mouse studies by gavage in mice (reviewed in Cruzan et al . (cdc.gov)
  • 2001). No other tumor types were increased in these toxicity in mice and rats, including mouse lung tu- studies in mice. (cdc.gov)
  • The PBPK model predicts that humans do not gener- toxicity, and nasal olfactory lesions. (cdc.gov)
  • PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat HSPH1 Monoclonal Antibody (Catalog # MAB4029) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007 ). (rndsystems.com)
  • Murine monoclonal antibodies are produced by injecting a mouse with an antigen, harvesting its spleen to obtain B cells that are producing antibody specific to that antigen, fusing those cells with immortal mouse myeloma cells, growing these hybridoma cells (eg, in cell culture), and harvesting the antibody. (msdmanuals.com)
  • To minimize the problems due to use of pure mouse antibody, researchers have used recombinant DNA techniques to create monoclonal antibodies that are part human and part mouse. (msdmanuals.com)
  • In both cases, the process usually begins as above with production of mouse hybridoma cells that make antibody to the desired antigen. (msdmanuals.com)
  • Then the DNA for some or all of the variable portion of the mouse antibody is merged with DNA for human immunoglobulin. (msdmanuals.com)
  • The resultant DNA is placed in a mammalian cell culture, which then expresses the resultant gene, producing the desired antibody. (msdmanuals.com)
  • Human NK cells developed in hIL-2 NOG mice express various NK receptors and produce both granzyme A and perforin upon stimulation. (taconic.com)
  • Studying NK1 receptors in the lab has the benefit of clinically available drugs that target the receptor, but also comes with challenges, as there are large differences between the NK1 receptor in mice and humans. (news-medical.net)
  • These mice were more resistant to pain than those with human NK1 receptors inside the cell, illustrating the importance of endosomes in signaling pain and the need for treatments that can penetrate cells. (news-medical.net)
  • In methylcellulose clonal culture of fetal liver (FL) cells of generated hGMR-expressing EPOR -/- embryos at embryonic day (E) 12.5 of gestation, hGM-CSF stimulated erythroid colony formation under serum-containing and serum-free conditions. (elsevierpure.com)
  • In the current study, they transplanted embryos of the most commonly used strain of laboratory mice for immune system research into female wild mice, who then gave birth to and raised wildlings. (sciencedaily.com)
  • Some prohibit only cloning for reproductive purposes and allow the creation of cloned human embryos for research, whereas others prohibit the creation of cloned embryos for any purpose. (who.int)
  • Researchers have developed a virus-based vaccine expressing a broad spectrum of undefined antigens from normal human prostate tissue that they claim completely eradicates established mouse prostate tumors in experimental animals. (genengnews.com)
  • Effective cancer immuno- and virotherapies are hindered by a lack of identified tumor-associated antigens (TAAs), poor coverage of the antigenic repertoire expressed by tumors, and difficulties associated with targeting vectors to tumors in vivo , explains Dr. Vile, together with co-lead author Alan Melcher, at the U.K. Leeds Institute of Molecular Medicine and Cancer Research U.K. Clinical Center, and colleagues. (genengnews.com)
  • They then went on to test the virus-expressed human cDNA library in mice with established prostate tumors. (genengnews.com)
  • Nine intravenous injections of ASEL were sufficient to cure over 80% of mice with the prostate tumors, with no detectable autoim-mune prostatitis. (genengnews.com)
  • They first tried a second vaccination comprising virally expressed immune-escape epitope libraries (IEELs) constructed using cDNA from the recurrent tumors of ASEL-treated mice. (genengnews.com)
  • BERLIN, Aug. 06, 2020 (GLOBE NEWSWIRE) -- T-knife GmbH, a next-generation adoptive T-cell company using its proprietary humanized T-cell receptor (HuTCR) mouse platform to treat solid tumors, announced today the closing of a €66 million Series A round of financing. (globenewswire.com)
  • T-knife is a next-generation adoptive T-cell company utilizing its proprietary humanized T-cell receptor (HuTCR) mouse platform technology to treat solid tumors. (globenewswire.com)
  • Together with the uniform over-expression of GCC in human tumors, and the standard of care in which hormone deficiencies are treated by replacement therapy, the role of GCC as a tumor suppressor underscores the potential of oral administration of GCC ligands for targeted prevention and therapy of colorectal cancer. (jefferson.edu)
  • Nevertheless, building on the positive aspects of this approach, the researchers postulated that it may be possible to provide a broad antigenic repertoire for in vivo immune selection of relevant tumor-associated antigens by expressing a cDNA library of a normal tissue using a systemically delivered, immunogenic vector. (genengnews.com)
  • They chose VSV as the vector for delivering and expressing the antigens in vivo. (genengnews.com)
  • Recombinant adeno-associated viruses (rAAVs) serve as vectors for in vivo gene delivery in both mice and humans, and have broad applicability for the treatment of genetic diseases. (umassmed.edu)
  • However, the in vivo validation of novel AAV constructs expressing products that act specifically on human cells and tissues is limited by a paucity of effective translatable models. (umassmed.edu)
  • Our TKI mouse model represents an important tool to test efficacy of human monoclonal Abs against SARS-CoV-2/related CoVs, predict human polyclonal Ab-mediated protection against newly emerging variants/CoVs, and dissect mechanisms of Ab-mediated protection in vivo. (aai.org)
  • Besides, their in vivo effect was explored using the established CNV mouse models. (hindawi.com)
  • Previous studies have proven that rodent and human MSCs can differentiate into HLCs in vitro and in vivo , which is one of the mechanisms of MSCs in the treatment of liver failure. (springer.com)
  • Moreover, The use of animals as surrogate rine host, can provide a platform for animal models for tumour viruses in hosts for the study of human tu- in vivo infection. (who.int)
  • For instance, mice are able to reconstitute most lymphomas in monkeys and humans woodchuck hepatitis virus induces major components of the human provides strong support for a direct hepatocellular carcinoma (HCC) haematolymphoid system including oncogenic role of EBV in vivo. (who.int)
  • Dr. Art Beaudet was recently granted nearly $28 million by the National Institutes of Health to create knock-out mouse models for every gene in the human genome, a testament to his expertise in animal models. (cureangelman.org)
  • In the new paper, researchers at the University of California, Santa Cruz compared the human genome to the genomes of chimps, dogs, rats, mice, and chickens, searching for genetic sequences that were highly conserved during evolution and therefore functionally important. (technologyreview.com)
  • Fluorescent microscopy of transiently transfected mammalian cells expressing Phi-Yellow proteins. (evrogen.com)
  • Fluorescent microscopy of stably transfected mammalian cells expressing PhiYFP in cytosol. (evrogen.com)
  • 1995]. When expressed in mammalian cells, this variant provides green fluorescent labeling of mitochondria. (evrogen.com)
  • Previous reports have indicated that CD163 is highly expressed on human macrophages, but found on less than 50% of peripheral blood monocytes. (nih.gov)
  • 99% of all CD14 positive monocytes express CD163 and that monocyte derived dendritic cells express low levels of CD163. (nih.gov)
  • We also show that IL-10, like glucocorticoids, induces high CD163 expression on cultured human monocytes. (nih.gov)
  • We contributed to this concept by showing that Tie2-expressing monocytes (TEMs) have a requisite role in this process. (europa.eu)
  • This vaccination regimen also led to the generation of both Th1 and Th2 cellular immune responses in mice. (frontiersin.org)
  • Our study indicates that co-expression of GP and VP40 antigens based on the SFV replicon vector generates EBOV VLPs in vitro , and vaccination with recombinant DREP vectors containing GP and VP40 antigens induces Ebola antigen-specific humoral and cellular immune responses in mice. (frontiersin.org)
  • Humanized mice that are engrafted with human cells, tissues, and immune systems offer strong potential to test the biological effectiveness of AAV vectors on human cells and tissues. (umassmed.edu)
  • These data recapitulate observations in clinical trials of IL-2 therapy and collectively show that humanized mouse models offer a translational platform for testing the efficacy of AAV vectors targeting human immune cells. (umassmed.edu)
  • In two preclinical studies, wildlings mirrored human immune responses, where lab mice failed to do so. (sciencedaily.com)
  • Our rationale was that the immune responses and microbiota of wild mice and humans are likely shaped in a similar way -- through contact with diverse microbes out in the real world. (sciencedaily.com)
  • Finally, the researchers tested how well the wildlings could predict human immune responses. (sciencedaily.com)
  • To do so, they drew from two studies where drugs used to target immune responses were successful in treating lab mice in preclinical trials but consequently failed to have therapeutic effects in humans. (sciencedaily.com)
  • We always strive for effective ways to shorten the gap between early lab findings and health advances in people, and the wildling model has the potential to do just that," said NIDDK Director Griffin P. Rodgers, M.D. "By helping to predict immune responses of humans, the wildling model could lead to important discoveries to help treat and prevent disease, and ultimately, improve human health. (sciencedaily.com)
  • Modulation of the Human Pancreatic Ductal Adenocarcinoma Immune Microenvironment by Stereotactic Body Radiotherapy. (rochester.edu)
  • Applications in research involving cancer, infectious disease, immunology, regenerative medicine and human immune system engraftment. (taconic.com)
  • Human renin is secreted by at least 2 cellular pathways: a constitutive pathway for the secretion of the precursor prorenin and a regulated pathway for the secretion of mature renin. (wikipedia.org)
  • icologic and metabolic data that explain the nasal and major differences are 4- to 10-fold more ring-oxidation lung differences and their relevance for human risk and phenylacetaldehyde pathways in mice compared assessment. (cdc.gov)
  • Differential requirement of ZAP-70 for CD2-mediated activation pathways of mature human T cells. (lu.se)
  • Vaccination of BALB/c mice with DREP-GP, DREP-VP40, or DREP-GP+DREP-VP40 vectors, followed by immediate electroporation resulted in a mixed IgG subclass production, which recognized EBOV GP and/or VP40 proteins. (frontiersin.org)
  • Phi-Yellow proteins can be easily expressed and detected in a wide range of organisms. (evrogen.com)
  • Heat Shock Proteins are ubiquitously expressed in all organisms. (rndsystems.com)
  • In this fashion, mice or other laboratory animals that exhibit particular traits can be created for specialized studies, or herds of farm animals (such as goats, sheep or cows) can be created that produce pharmaceutically useful proteins in their milk. (who.int)
  • Gene-trap mutagenesis using Mol/MSM-1 embryonic stem cells from MSM/Ms mice. (nih.gov)
  • Western blot shows lysates of HT-29 human colon adeno-carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Nb2-11 rat lymphoma cell line untreated (-) or treated (+) with heat shock at 42 °C for 30 minutes and allowed to recover at 37 °C for 3 hours. (rndsystems.com)
  • Chimeric monoclonal antibodies activate antigen-presenting cells (APCs) and T cells more effectively than murine monoclonal antibodies but can still induce production of human anti-chimeric antibodies. (msdmanuals.com)
  • Susceptibility to hepatotoxicity in transgenic mice that express a dominant-negative human keratin 18 mutant. (jci.org)
  • To assess the susceptibility of K18-hACE2 mice to SARS-CoV-2, we intranasally challenged male and female mice using inocula of 10 3 , 10 4 and 10 5 plaque-forming units (PFU). (nature.com)
  • Transgenic mice expressing porcine PrP were, nevertheless, completely resistant to infection with a broad panel of classical scrapie isolates from different sheep PrP genotypes and with different biochemical characteristics. (cdc.gov)
  • However, the experimental inoculation of pigs and transgenic mice overexpressing porcine PrP has indicated that swine are susceptible to BSE infection by the parenteral route, although with a considerable transmission barrier ( 8 , 9 ). (cdc.gov)
  • The potential spread of BSE to animals in the human food chain such as sheep, goats, and pigs needs assessing because a risk for human infection by animals other than BSE-infected cattle cannot be excluded. (cdc.gov)
  • Here, using K18-hACE2 transgenic mice that were originally developed for SARS studies 4 , we show that infection with SARS-CoV-2 causes severe disease in the lung and, in some mice, the brain. (nature.com)
  • Mice developed anosmia at early time points after infection. (nature.com)
  • On the basis of these observations, we reasoned that SARS-CoV-2 infection of the K18-hACE2 mice might also show differences in pathogenesis. (nature.com)
  • To understand the cause(s) of this morbidity and mortality better, we assessed the tissue distribution of infectious virus and viral genomic RNA at 2, 4 and 6 days post infection (dpi) in mice receiving 10 5 PFU of SARS-CoV-2. (nature.com)
  • Variability in brain infection was also described in a recent study of K18-hACE2 mice infected with SARS-CoV-2 11 . (nature.com)
  • Here, we successfully developed a SARS-CoV-2 hACE2 transgenic mouse (HFH4-hACE2 in C3B6 mice ) infection model. (bvsalud.org)
  • Viral quantification revealed the lungs as the major site of infection , although viral RNA could also be found in the eye , heart , and brain in some mice . (bvsalud.org)
  • A human anti-SARS-CoV-2 IgG1 with a half-life extending mutation but not wild-type IgG1 exhibited long half-life and protected against BA.2 infection in lungs, thereby validating our TKI mice for evaluating human Ab clearance and function. (aai.org)
  • Treatment with pooled plasma from vaccination plus infection but not triple vaccination individuals cleared infectious Delta in lungs of TKI mice, although Delta-binding and -neutralizing Ab titers in vitro were similar between the two groups of people. (aai.org)
  • Thus, Abs elicited by vaccination plus infection but not triple vaccination reduce Delta infection in this mouse model, and Abs from both groups are ineffective against BA.5. (aai.org)
  • In 2017, they led research showing that transferring wild mice gut microbiota into lab mice helped the mice survive an otherwise lethal flu virus infection and fight colorectal cancer. (sciencedaily.com)
  • genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)
  • Researchers developed a new mouse model that could improve the translation of research in mice into advances in human health. (sciencedaily.com)
  • The researchers and their collaborators compared the microbiota of the wildlings, wild mice and lab mice. (sciencedaily.com)
  • In the current study, the researchers treated wildlings and lab mice with the same drugs. (sciencedaily.com)
  • Researchers have engineered mice to express an additional photoreceptor, a transformation that may mimic the evolution of trichromatic vision in primates, reports this week's Science . (the-scientist.com)
  • Researchers from Harvard Medical School have teamed together to pin-point the neural pathway linked to human longevity. (express.co.uk)
  • To investigate the link between neural excitation and lifespan further, the researchers experimented on mice. (express.co.uk)
  • Interestingly, the researchers found that the brains of human centenarians had significantly more REST in the nuclei of their brain cells than those who died in their 70s. (express.co.uk)
  • To overcome this, the researchers genetically modified mice to express the human NK1 receptor. (news-medical.net)
  • In another experiment, the researchers studied mice with a different type of NK1 receptor on the outer membrane of the cell, rather than inside. (news-medical.net)
  • The researchers identified several rapidly evolving chunks of DNA, but the fastest piece by far was a small chain of DNA that's part of a gene expressed in the hippocampus, which is involved in learning and memory. (technologyreview.com)
  • The researchers then discovered that the gene, known as HAR1F, has some even more enticing properties: it is expressed in a particular set of cells in the human brain between 9 and 19 weeks of gestation - when the cortex is undergoing a rapid period of development. (technologyreview.com)
  • The BACHD mouse model expressing full-length mutant HTT does not display any hypothalamic neuropathology at 2 months of age. (lu.se)
  • Effects of mutant huntingtin inactivation on Huntington disease-related behaviours in the BACHD mouse model. (lu.se)
  • Imbalance of the oxytocin-vasopressin system contributes to the neuropsychiatric phenotype in the BACHD mouse model of Huntington disease. (lu.se)
  • We recently described transgenic mice that express point-mutant human K18 (Ku, N.-O., S. Michie, R.G. Oshima, and M.B. Omary. (jci.org)
  • Here we show that mutant K18 expressing transgenic mice are highly susceptible to hepatotoxicity after acute administration of acetaminophen (400 mg/Kg) or chronic ingestion of griseofulvin (1.25% wt/wt of diet). (jci.org)
  • To address this issue, human granulocyte-macrophage colony-stimulating factor (hGM-CSF) receptor (hGMR)-transgenic mice and heterozygous EPOR mutant mice were crossed by in vitro fertilization. (elsevierpure.com)
  • Experimental Pharmacology & Oncology (EPO) GmbH uses 1st and 2nd generation NOG mice provided by Taconic for its preclinical oncology service. (taconic.com)
  • I will now implement preclinical models, including human hematochimeric mice, that will better assess the safety and feasibility of this new delivery strategy. (europa.eu)
  • Blue cells = expressed in wild-type. (jax.org)
  • In stratum oriens of the hippocampus, EGFP-expressing interneurons were comprised almost exclusively of oriens/alveus interneurons with lacunosum-moleculare axon arborization (O-LM cells). (jneurosci.org)
  • In hippocampal area CA1, two previously uncharacterized subtypes of interneurons were identified using the GIN mice: stratum pyramidale interneurons with lacunosum-moleculare axon arborization (P-LM cells) and stratum radiatum interneurons with lacunosum-moleculare axon arborization (R-LM cells). (jneurosci.org)
  • Using the BLT (bone marrow, liver, thymus) humanized NOD-scid Il2rgnull (NSG) mouse model, which enables efficient development of HLA-restricted effector and regulatory T cells (Tregs), we have evaluated the delivery and function of human interleukin (IL)-2 by an AAV vector. (umassmed.edu)
  • Numbers of activated human natural killer cells were also increased significantly in humanized mice treated with the IL-2 vector. (umassmed.edu)
  • K18-hACE2 mice express hACE2 driven by the cytokeratin 18 ( KRT18 ) promoter, predominantly in epithelial cells 8 . (nature.com)
  • All together, the results of the present study demonstrated that hGM-CSF can stimulate the proliferation and differentiation of primitive and definitive erythroid cells independently of EPOR signal if they express hGMR, and the activity is comparable to that of EPO in definitive, but not primitive, erythropoiesis. (elsevierpure.com)
  • HumanKine IFN-α 2A, expressed in human HEK 293 cells, is a glycosylated monomer with an apparent molecular mass of 16 kDa. (sigmaaldrich.com)
  • Production in human HEK 293 cells offers authentic glycosylation. (sigmaaldrich.com)
  • Highly expressed LCN2, SOX9, and SIRT1 were observed in hypoxic cells. (hindawi.com)
  • LCN2 was increased by SOX9 and SIRT1 deacetylated SOX9 to promote its nuclear translocation, which further inhibited the viability of human retinal pigment epithelial cells and promoted cell apoptosis and angiogenesis as well as CNV-induced AMD formation. (hindawi.com)
  • H) T24 cells expressing destabilized variant PhiYFP-m-dest1. (evrogen.com)
  • Predominant differentiation of human NK cells following human HSC engraftment, with>10-fold higher CD56+ NK cell numbers compared to the base NOG mouse. (taconic.com)
  • Based on user feedback, this level appears sufficient to support engraftment of primary human T cells. (taconic.com)
  • mouse: an excellent recipient mouse model for engraftment of human cells. (taconic.com)
  • It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. (biolegend.com)
  • Plasma cells do not express CD19. (biolegend.com)
  • Recently, signaling by GCC and its downstream effector, cyclic GMP (cGMP) has emerged as a principal regulator of proliferation in human colon cancer cells. (jefferson.edu)
  • We found that myocilin is expressed in bone marrow-derived mesenchymal stem cells (MSCs) and affects their differentiation into osteoblasts. (nih.gov)
  • newborn and weaning mice, primary chick embryo cell cultures and C6/36 clone of Aedes albopictus cells. (cdc.gov)
  • that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)
  • Zoster provides a unique opportunity to determine if focal reinfection of human skin boosts local or disseminated antigen-specific tissue-resident T cells. (cdc.gov)
  • Alternative antigen receptor (TCR) signaling in T cells derived from ZAP-70-deficient patients expressing high levels of Syk. (lu.se)
  • In one of the resulting transgenic lines, called GIN ( G FP-expressing I nhibitory N eurons), EGFP was found to be expressed in a subpopulation of somatostatin-containing GABAergic interneurons in the hippocampus and neocortex. (jneurosci.org)
  • Due to their natural generation in mice without negative thymic selection, these TCRs are of high specificity and high affinity. (globenewswire.com)
  • Defective T cell receptor signaling and CD8+ thymic selection in humans lacking zap-70 kinase. (lu.se)
  • Microarray profiling of hypothalamic gene expression changes in Huntington's disease mouse models. (lu.se)
  • OPV/Sabin infected animals including, but not limited to, non-human primates and transgenic mice expressing the human PV receptor (CD155). (cdc.gov)
  • in styrene metabolism between rats and mice. (cdc.gov)
  • The Prkdc scid mutation was identified by Mel Bosma of the Fox Chase Cancer Center in a C.B-17 congenic mouse population. (taconic.com)
  • The CIEA NOG mouse ® was developed by backcrossing the C57BL/6JJic-Il2rg line to the NOD/ShiJic-Prkdc scid line for a total of eight generations. (taconic.com)
  • Fukuchi Y, Miyakawa Y, Kobayashi K, Kuramochi T, Shimamura K, Tamaoki N, Nomura T, Ueyama Y, Ito M. (1998) Cytokine dependent growth of human TF-1 leukemic cell line in human GM-CSF and IL-3 producing transgenic SCID mice. (taconic.com)
  • Ebola virus (EBOV) causes severe hemorrhagic fevers in humans, and no approved therapeutics or vaccine is currently available. (frontiersin.org)
  • Studies in animals infected experimentally with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the cause of COVID-19, provide opportunities to study aspects of the disease that are not easily investigated in human patients. (nature.com)
  • Evidence of thrombosis and vasculitis was detected in mice with severe pneumonia. (nature.com)
  • Last, we showed that pre-exposure to SARS-CoV-2 could protect mice from severe pneumonia . (bvsalud.org)
  • Human severe combined immunodeficiency due to a defect in ZAP-70, a T cell tyrosine kinase. (lu.se)
  • Thus, K18-hACE2 mice provide a useful model for studying the pathological basis of both mild and lethal COVID-19 and for assessing therapeutic interventions. (nature.com)
  • The mouse model, which the scientists called 'wildling,' acquired the microbes and pathogens of wild mice, while maintaining the laboratory mice's genetics that make them more useful for research. (sciencedaily.com)
  • We wanted to create a mouse model that better resembles a mouse you'd find in the wild," said Barbara Rehermann, M.D., chief of the Immunology Section in NIDDK's Liver Diseases Branch and senior author on the study. (sciencedaily.com)
  • The MPTP-treated mouse has been the most widely used model for assessing neuroprotective agents for Parkinson's disease. (researchgate.net)
  • There was no effect of hypothalamic injections of QA on the neuronal populations expressing orexin, MCH, CART or oxytocin in neither HD mouse model. (lu.se)
  • IKKβ signaling mediates metabolic changes in the hypothalamus of a Huntington's disease mouse model. (lu.se)
  • The expression of human IL-2 did not significantly change the levels or activation status of conventional T-cell subsets. (umassmed.edu)
  • The activity is determined by the dose-dependent cytotoxicity of the human TF-1 cell line (human erythroleukemic indicator cell line)) ≤ 0.4. (sigmaaldrich.com)
  • Tenascin-R mRNA was expressed by distinct neural cell types in the unlesioned olivocerebellar system. (researchgate.net)
  • Crypt hyperplasia in GCC-/- mice was associated with compensatory increases in cell migration and apoptosis. (jefferson.edu)
  • Animal models for human tumour mental animals is not easy to answer does induce adult T-cell leukaemia/ viruses that make use of animal virus- for these agents, because cancer bi- lymphoma (ATLL), albeit in monkeys es are scarce. (who.int)
  • The skin T cell infiltrates express the canonical tissue-resident T cell markers CD69 and CD103. (cdc.gov)
  • Compensating for the loss of ClpP in the substantia nigra of αSyn A53T mice by viral expression of ClpP suppresses mitochondrial oxidative damage, and reduces αSyn pathology and behavioral deficits of mice. (springer.com)
  • These in vitro, and their expression in these human tumour virus. (who.int)
  • The team cloned a cDNA library from normal human prostate, which they termed altered self antigen and epitope library (ASEL) into VSV, and systemically injected the antigen-expressing vector into normal experimental mice. (genengnews.com)
  • Humanized mice treated with an AAV vector expressing human IL-2 showed a significant and sustained increase in the number of functional human FOXP3+CD4+ Tregs. (umassmed.edu)
  • The hIL-2 NOG mouse was developed by microinjecting a transgene consisting of a DNA fragment containing human IL-2 cDNA under the control of the CMV promoter into zygotes of NOD/ShiJic-Il2rg mice. (taconic.com)
  • and KChIP3, also known as calsenilin and DREAM (downstream regulatory elements (DRE)-antagonist modulator) which is expressed in the brain and testis. (fishersci.com)
  • sponse of the nasal olfactory mucosa, with the mouse metabolism studies have indicated major differences being much more sensitive. (cdc.gov)
  • The CIEA NOG mouse ® was developed by Mamoru Ito of the Central Institute for Experimental Animals (CIEA) in Japan. (taconic.com)
  • SOX9 belongs to the SOX gene family expressed in several organisms and involved in numerous physiological processes such as sex determination and development gonad [ 11 ]. (hindawi.com)
  • To further the potential therapeutic options for turning on the paternal allele, as with Antisense Oligonucleotides (ASOs) or Artificial Transcription factors (ATF's or Zinc Fingers), knowing the exact genetic makeup of the human antisense transcript is very important. (cureangelman.org)
  • Injecting purified human PON1-Q or RI92 restores resistance, with either alloform protecting equivalently against DZO exposure and PON1192 providing greater protection against CPO exposure. (cdc.gov)
  • Reports that establish associations between naphthalene exposure and health effects in humans are restricted to numerous reports of hemolytic anemia or cataracts following acute exposure or occupational exposure to naphthalene, either by ingestion or by inhalation of naphthalene vapors, but these reports have not identified exposure levels associated with these effects. (cdc.gov)
  • The infected mice generated typical interstitial pneumonia and pathology that were similar to those of COVID-19 patients . (bvsalud.org)
  • The predisposition to hepatotoxicity results directly from the keratin mutation since nontransgenic or transgenic mice that express normal human K18 are more resistant. (jci.org)
  • Since the sequences of the human and other animal genomes have become available for study, scientists have been scouring reams of DNA for genetic clues to our brains' unique growth spurt - and to the biological changes that make us uniquely human. (technologyreview.com)
  • Building upon my previous studies, I will provide a thorough characterization of the precise identity of TEMs and of their biological role in mouse tumor models. (europa.eu)
  • Differential promoter methylation and histone modification contribute to the brain specific expression of the mouse Mbu-1 gene. (nih.gov)
  • Pathogenesis of SARS-CoV-2 in Transgenic Mice Expressing Human Angiotensin-Converting Enzyme 2. (bvsalud.org)
  • The normal concentration of renin in adult human plasma is 1.98-24.6 ng/L in the upright position. (wikipedia.org)
  • There was variable mortality when mice were inoculated with 10 4 PFU (2 out of 3 female mice and 3 out of 7 male mice survived). (nature.com)
  • Please note that advanced order placement is encouraged for male mice. (taconic.com)