Mice epithelial cells. Medical search. Frequent questions

The EasySep™ Mouse Epithelial Cell Enrichment Kit II is designed to isolate epithelial cells from freshly dissociated mouse mammary tissues by immunomagnetic negative selection. (stemcell.com)
in fact, EpCAM is expressed in a variety of human epithelial tissues, carcinomas, and progenitor and stem cells. (wikipedia.org)
Active proliferation in a number of epithelial tissues is associated with increased or de novo EpCAM expression. (wikipedia.org)
RT-PCR analysis , co-immunoprecipitation , native PAGE , mass spectrometry , and differential centrifugation were combined with high-resolution immunofluorescence histochemistry, proximity ligase assays, and stimulated emission depletion (STED) microscopy on mouse choroid plexus cells or tissues in order to resolve these issues. (bvsalud.org)
The ordered expression of αIEL during thymocyte development (which occurs under the influence of the thymic epithelium), high level of αIEL expression on peripheral T cells in epithelial tissues (IEL and DEC), and expression of CD103 on a subset of CD8+ lymphocytes responding to allogeneic epithelial cells, suggest that αIELβ7 integrin may have a common role in the interactions of T lymphocytes with epithelia during T-cell maturation and effector functions. (bdbiosciences.com)
COX-1 is expressed constitutively and is isolated throughout most cell lines in almost all mammalian tissues. (medscape.com)
The three tested African strains and one European strain from Spain caused 100% mortality in inoculated mice, with similar survival times and histopathology in tissues. (cdc.gov)
Unexpectedly, a European strain from the Netherlands caused only 12% mortality and significantly less histopathology in tissues from mice compared to mice inoculated with the other strains. (cdc.gov)
Tissues and Cells. (lu.se)

Human hematopoietic cells and thymic epithelial cells induce tolerance via different mechanisms in the SCID-hu mouse thymus. (rupress.org)
Immunohistology of the thymus grafts demonstrated that thymic epithelial cells were of fetal thymus donor (FTD) origin. (rupress.org)
These data demonstrate that T cells become tolerant to autologous and allogeneic HLA antigens expressed in the thymus via two different mechanisms: hematopoietic cells present in the thymus induce tolerance to "self"-antigens by clonal deletion, whereas thymic epithelial cells induce tolerance by clonal energy and possibly deletion of high affinity clones. (rupress.org)
For example, mouse thymic epithelial cells react with antibodies for keratin 5, keratin 8, and keratin 14. (wikipedia.org)
These antibodies are used as fluorescent markers to distinguish subsets of mouse thymic epithelial cells in genetic studies of the thymus . (wikipedia.org)

Since oxidative stress in airway cells increases platelet-activating factor receptor (PAFR) expression, and PAFR is co-opted by pneumococci to adhere to host cells, we hypothesised that E-cigarette vapour increases pneumococcal adhesion to airway cells. (ersjournals.com)
We determined the effect of vapour on oxidative stress-induced, PAFR-dependent pneumococcal adhesion to airway epithelial cells in vitro , and on pneumococcal colonisation in the mouse nasopharynx. (ersjournals.com)
Nicotine-containing and nicotine-free E-cigarette vapour increased pneumococcal adhesion to airway cells in vitro . (ersjournals.com)
To establish firm adhesion, pneumococci co-opt host-expressed platelet-activating factor receptor (PAFR), and then use the receptor as a Trojan horse to enter airway cells as the receptor is internalised [ 7 ]. (ersjournals.com)
For example, we found that cigarette smoke extract, fossil fuel-derived particulate matter and welding fumes, by means of the induction of oxidative stress, upregulate PAFR-dependent pneumococcal adhesion to lower airway cells in vitro [ 8 - 10 ]. (ersjournals.com)
Because of ethical and experimental constraints and more important, to reduce and refine animal experimentation, we used our repository of well-differentiated airway epithelial cell (AEC) cultures from various domesticated and wildlife animal species to assess their susceptibility to SARS-CoV-2. (cdc.gov)
Here, using in vitro human and mouse airway epithelial cell models and in vivo mouse infection, we found that the α2.3-linked SA receptor was expressed in ciliated airway and type II alveolar epithelial cells and was targeted for cell-specific infection in both species. (elsevierpure.com)
In human airway epithelial cells, α2,6-linked SA was expressed and functional in both ciliated and goblet cells, providing expanded cellular tropism. (elsevierpure.com)
Differences in receptor and cell-specific expression in these species suggest that differentiated human airway epithelial cell cultures may be superior for evaluation of some human strains, while the mouse can provide a model for studying avian strains that preferentially bind only the α2,3-linked SA receptor. (elsevierpure.com)
Airway progenitor cells contain two parental centrioles (PC) and form structures called deuterosomes that nucleate centrioles during amplification. (elifesciences.org)
Title : Effect of Epithelial-Specific MyD88 Signaling Pathway on Airway Inflammatory Response to Organic Dust Exposure Personal Author(s) : Johnson, Amber N.;Dickinson, John;Nelson, Amy;Gaurav, Rohit;Kudrna, Katrina;Evans, Scott E.;Janike, Katherine;Wyatt, Todd A.;Poole, Jill A. (cdc.gov)

Troy+ cells have a 40-fold higher capacity than Troy- cells to form organoids, which is considered a stem cell property in vitro. (knaw.nl)
In this report, we show that infection with influenza A virus and treatment with the resulting host signals (febrile-range temperatures, norepinephrine, extracytoplasmic ATP, and increased nutrient availability) induce the release of bacteria from biofilms in a newly developed biofilm model on live epithelial cells both in vitro and during in vivo colonization. (lu.se)
MLE-12 cells were induced by LPS to construct an ARDS model in vitro. (karger.com)
METHODS: As bronchial epithelial cells (BECs) are pivotal in initiating organic dust-induced inflammation, we investigated the in vitro effects of MaR1 on a human BEC cell line (BEAS-2B). (cdc.gov)
In conclusion, the presented mouse models of colorectal cancer further strengthen our previous in vitro findings and highlight the prospect of CysLT1R as an alternative therapeutic approach. (lu.se)

Wnt ligands are secreted glycoproteins that function in intracellular signaling pathways to regulate a variety of developmental processes including cell growth, cell differentiation, cell polarity, and apoptosis. (biomedcentral.com)
Gata-3 is an essential regulator of mammary-gland morphogenesis and luminal-cell differentiation. (edu.au)
The AF form of SALSA had a more intact structure and contained peptides from the zona pellucida domain, which is involved in cell differentiation and oligomerization. (researchgate.net)
CD24 is involved in regulating B cell apoptosis and in preventing terminal differentiation of B cells into antibody-secreting plasmablasts and plasma cells. (stemcell.com)
However, a major unresolved question is how each cell regulates the precise number of its motile cilia during differentiation. (elifesciences.org)

The stem/progenitor potential of these cell populations was assessed in vivo by cleared mammary fat pad transplantation. (cardiff.ac.uk)
During kidney development, progressively committed progenitor cells give rise to the distinct segments of the nephron, the functional unit of the kidney. (knaw.nl)
Similar segment-committed progenitor cells are thought to be involved in the homeostasis of adult kidney. (knaw.nl)
However, markers for most segment-committed progenitor cells remain to be identified. (knaw.nl)
Here, we evaluate Troy/TNFRSF19 as a segment-committed nephron progenitor cell marker. (knaw.nl)
Our data show that Troy marks a renal stem/progenitor cell population in the developing kidney that in adult kidney contributes to homeostasis, predominantly of the collecting duct, and regeneration. (knaw.nl)
Mouse utricle sensory epithelial cellCderived progenitor cells (MUCs), which possess locks cell progenitor and mesenchymal features via epithelial-to-mesenchymal changeover (EMT) seeing that previously described, provide a potential strategy for locks cell regeneration via cell transplantation. (forgetmenotinitiative.org)
In the auditory system, we have generated mouse utricle sensory epithelial cellCderived progenitor cells (MUCs) via EMT (Zhang and Hu, 2012). (forgetmenotinitiative.org)
1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)

The definitive identification of a mammary epithelial stem cell population is critically dependent on its purity. (cardiff.ac.uk)
Several functional components of the epithelium contribute to this unique role, among them the secretion of a mucus layer and life-long regeneration of the different epithelial cell types from a stem cell compartment, allowing for quick repair of barrier defects 2 . (nature.com)
Recently, cell reprogramming technology has been used in stem cell research. (forgetmenotinitiative.org)
Zhao Y.(2011).Mouse embryonic stem cell-derived thymic epithelial cell progenitors enhance T-cell reconstitution after allogeneic bone marrow transplantation. (ioz.ac.cn)
Scientific Advisory Boards: National Breast Cancer Foundation, National Stem Cell Foundation of Australia and Cancer Science Institute (Singapore), Victorian Cancer Biobank, Pfizer, AbbVie. (edu.au)
Board Member: National Stem Cell Foundation of Australia, Executive member kConFab (Kathleen Cuningham Foundation Consortium for research into Familial Breast cancer). (edu.au)
Generation of a functional mammary gland from a single stem cell. (edu.au)
Two theories predominate: the bicellular stem cell theory and the multicellular theory. (medscape.com)
Recent evidence suggests that the bicellular stem cell theory is the more probable etiology of salivary gland neoplasms. (medscape.com)

The breast is a complex tissue consisting of two epithelial layers (an outer myoepithelial/basal layer and an inner luminal epithelial layer) as well as a large non-epithelial component (fibroblasts, endothelial cells, lymphocytes, adipocytes, neurons and myocytes). (cardiff.ac.uk)
It is expressed on almost all intestinal intraepithelial lymphocytes (IEL), dendritic epidermal T cells (DEC), subpopulations of peripheral T cells, and distinct subsets of fetal, neonatal, and adult thymocytes. (bdbiosciences.com)
Sivelestat significantly inhibited the increase in lung collagen content, fibrotic changes, the numbers of total cells (including macrophages, neutrophils and lymphocytes), the levels of the active form of TGF-β1 and phospho-Smad2 in bleomycin-induced early-stage pulmonary fibrosis. (ersjournals.com)

Genetic deletion of Dnmt3a in intestinal epithelial cells ( Dnmt3a ΔIEC ) in mice confirms the phenotype of an altered epithelial ultrastructure with shortened apical-junctional complexes, reduced Goblet cell numbers and increased intestinal permeability in the colon in vivo. (nature.com)
These data offer insights for a novel mechanistic action of MaR1 in bronchial epithelial cells, and support future in vivo studies to test MaR1's utility in reducing the deleterious inflammatory effects of environmental dust exposures. (cdc.gov)
Objective: This in vivo experimental study evaluated the effect of methotrexate on the oral mucosa of mice. (bvsalud.org)

A new study in mice led by Kathryn Anderson , Chair of the Developmental Biology Program in the Sloan Kettering Institute, showed that a mutation in the PTEN gene that's been linked to many kinds of cancer can also cause birth defects of the nervous system. (mskcc.org)
9 Department of Cell and Developmental Biology and Program in Chemical and Physical Biology, Vanderbilt University School of Medicine, Nashville, Tennessee. (aacrjournals.org)

This will considerably facilitate the further analysis of mammary epithelial subpopulations, whilst ensuring high purity, which is key for understanding mammary epithelial stem cells in normal tissue biology and carcinogenesis. (cardiff.ac.uk)
Our scientists pursue every aspect of cancer research-from exploring the biology of genes and cells, to developing immune-based treatments, uncovering the causes of metastasis, and more. (mskcc.org)
I am a clinician-scientist and Joint Head of the ACRF Cancer Biology and Stem Cells Division and Breast Cancer Laboratory. (edu.au)
The laboratory mouse is an important model for addressing questions in cancer biology. (cshlpress.com)
BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)

These animals were studied between 7 and 12 mo after transplantation, at which times all thymocytes and peripheral T cells were derived from stem cells of the fetal liver graft. (rupress.org)
Thymocytes of SCID-hu mice transplanted with liver and thymus of two different donors (FLDA/FTDB animals) were nonresponsive to Epstein-Barr virus-transformed B cell lines (B-LCL) established from both the FLDA and FTDB, but proliferated vigorously when stimulated with third-party allogeneic B-LCL. (rupress.org)

The EasySep™ procedure involves labeling unwanted non-epithelial cells with biotinylated antibodies and magnetic particles. (stemcell.com)

Intestinal epithelial cells (IECs) have evolved to build a complex physiological barrier that separates the intestinal lumen from the underlying mucosal tissue. (nature.com)
Single layered intestinal epithelial cells (IECs) establish a semipermeable intestinal barrier (IB) which allows the dual role of transporting nutrients and avoiding the passage of pathogens. (frontiersin.org)
Selective permeability of IECs is achieved by apical junctional complex (AJC) proteins which comprise tight junctions, adherens junctions, and desmosomes to maintain the apicobasal cell polarity and intestinal tissue integrity ( 4 ). (frontiersin.org)

In this study, the protective effect of a fermented wheat germ extract (FWGE) against LPS-induced inflammation and oxidative stress in IPEC-J2 porcine intestinal epithelial cells was studied. (hindawi.com)

Shi and Advantage 2013), and locks cell progenitors possess been determined in the adult mammalian utricle and cochlea (Li et al. (forgetmenotinitiative.org)
2007). Some research possess recommended that assisting cells had been the most likely resource of progenitors (Kelley et al. (forgetmenotinitiative.org)
Remarkably, these mesenchymal-like MUCs expressed prosensory cell markers, which indicated that MUCs were prosensory-like hair cell progenitors that may be able to differentiate into new hair cells. (forgetmenotinitiative.org)
Prosensory cells are hair cell progenitors because they differentiate into hair and supporting cells during development (Kelley, 2006). (forgetmenotinitiative.org)

The α2.6-linked SA receptor was not expressed in the mouse, a factor that may contribute to the inability of some human strains to efficiently infect the mouse lung. (elsevierpure.com)
Alternatively, a mouse lung slice model was utilized in supportive cytokine studies. (cdc.gov)
MaR1 also reduced HDE-stimulated cytokine release including TNF-a in a mouse lung slice model when given before or following HDE treatment. (cdc.gov)
These results suggest that sivelestat alleviated bleomycin-induced pulmonary fibrosis via inhibition of both TGF-β activation and inflammatory cell recruitment in the lung. (ersjournals.com)
icologic and metabolic data that explain the nasal and major differences are 4- to 10-fold more ring-oxidation lung differences and their relevance for human risk and phenylacetaldehyde pathways in mice compared assessment. (cdc.gov)
lung Clara cells and nasal olfactory cells (Cruzan et al . (cdc.gov)
in an inhalation study and only equivocal evidence of metabolism to SO is below the limit of detection in nearly all samples, and the most active sample of lung lung tumors in two of four chronic toxicity/oncogenicity was approximately 100-fold less active than mouse studies by gavage in mice (reviewed in Cruzan et al . (cdc.gov)
2001). No other tumor types were increased in these toxicity in mice and rats, including mouse lung tu- studies in mice. (cdc.gov)

These Wnts were chosen since previous studies suggest that C57mg cells respond differently to these Wnts, and since these Wnts can activate different signaling pathways in other systems. (biomedcentral.com)

DNMT3A expression is downregulated in intestinal epithelial cells from IBD patients and upon tumor necrosis factor treatment in murine intestinal organoids. (nature.com)

Conclusion: Differential staining of mammary epithelial cells for CD24 can be used to simultaneously isolate pure populations of non-epithelial, myoepithelial/basal and luminal epithelial cells. (cardiff.ac.uk)
The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)

To study the role of thymic education on the development of the human T cell repertoire, SCID-hu mice were constructed with fetal liver and fetal thymus obtained from the same or two different donors. (rupress.org)
Dendritic cells and macrophages of fetal liver donor (FLD) origin were abundantly present in the medullary and cortico-medullary areas. (rupress.org)

2007). Such a dedifferentiation program occurs in mammalian pancreatic epithelial cell cultures, in which pancreatic cells dedifferentiate into mesenchymal-like cells that are able to re-enter the cell cycle, probably via epithelial-to-mesenchymal transition (EMT) (Gallo et al. (forgetmenotinitiative.org)
We found that adult mouse utricle sensory epithelial cells underwent EMT to become MUCs that were able to propagate in adherent cultures, express mesenchymal markers, and lose the expression of epithelial markers. (forgetmenotinitiative.org)
In suspension cultures, we found that MUCs formed spherical structures buy SB-649868 and started to express epithelial markers and bundle-like structures, which indicates that MUCs may possess the potential to become epithelial hair-like cells. (forgetmenotinitiative.org)
However, the yield of epithelial bundleCbearing cells is limited in MUC suspension cultures and/or hanging drops. (forgetmenotinitiative.org)
In this study, we maintained mesenchymal-like MUCs in adherent cultures to test new methods that were able to guide MUCs to buy SB-649868 become a number of epithelial cells expressing epithelial genes such as in MUCs. (forgetmenotinitiative.org)

Our group is studying molecular regulators of normal mammary gland development and cancer, with a particular interest in understanding how mammary stem cells and their progeny contribute to the mammary epithelial cell hierarchy and breast cancer. (edu.au)

Innate immunity in gastrointestinal system includes epithelial cell layers that express tight cell-cell contact, secreted mucous layer which prevent entry of microbes, protective plasma protein, and circulating leukocytes, such as phagocytic macrophages, neutrophils, dendritic cells (DC), natural killer (NK) cells, NK T-cell, and innate lymphoid cells. (frontiersin.org)

some cells in a tumor have more EpCAM than other cells in the same tumor. (wikipedia.org)
Using this technique, the tumor cells grow within the mammary fat pad, in the appropriate tissue environment, mimicking human breast cancer progression more closely than subcutaneously injection or xenograft to mice cells. (jove.com)
Clear cell renal cell carcinoma (ccRCC) is characterized by a loss of the tumor suppressor gene VHL, and hence this tumor type is characterized by constitutive activation of the hypoxic response. (lu.se)
In the multicellular theory, each tumor type is associated with a specific differentiated cell of origin within the salivary gland unit. (medscape.com)
In the mouse xenograft model of colon cancer, we were able to observe a reduced tumor growth in response to CysLT1R antagonist treatment. (lu.se)
Also, a decreased nuclear expression of β-catenin within the epithelial tumor compartment was determined for CysLT1R mutant female mice in both models. (lu.se)
However, the mechanism of tumor progression in FAP/sporadic colorectal cancer and in colitis-associated colorectal cancer might differ as indicated by reduced tumor expression of COX-2 and reduced serum levels of PGE2 in the female double mutant (CysLT1R−/− ApcMin/+) mice, whereas AOM/DSS-treated female single mutant (CysLT1R−/−) mice demonstrated increased serum levels of PGE2. (lu.se)

and negative regulation of cell population proliferation. (nih.gov)
We have shown that LTD4 via its receptor CysLT1 induces expression of proteins associated with colorectal cancer and promotes proliferation, survival and migration in intestinal epithelial cells. (lu.se)

Furthermore, LPS-triggered partial disruption of epithelial integrity was reduced after FWGE application. (hindawi.com)
Pretreatment with C-BF prevented LPS induced intestinal tight junction disruption and epithelial cell apoptosis. (unboundmedicine.com)

Dnmt3a ΔIEC mice suffer from increased susceptibility to experimental colitis, characterized by reduced epithelial regeneration. (nature.com)

Cells were sorted into separate populations based on CD24 expression and assessed for luminal epithelial and myoepithelial/basal markers by direct fluorescent microscopy and real time PCR. (cardiff.ac.uk)
There was no difference in baseline nasal epithelial PAFR expression between vapers (n=11) and controls (n=6). (ersjournals.com)
Nicotine-containing E-cigarette vapour increased mouse nasal PAFR expression, and nasopharyngeal pneumococcal colonisation. (ersjournals.com)
In this work, we analyze expression patterns of DNMT3A in patients with IBD, perform loss of function experiments using Caco-2 cell line and generate a conditional Dnmt3a ΔIEC mouse line. (nature.com)
Expression levels are substantially higher in small intestine, and in colon EpCAM is probably expressed at the highest levels among all epithelial cell types. (wikipedia.org)
EpCAM expression differs between different types of renal cell carcinomas, and EpCAM expression increases during development of androgen resistance in prostate cancer. (wikipedia.org)
To test the hypothesis that a specific cell line can respond to distinct Wnts with different patterns of gene expression, we over-expressed Wnt-5a and Rfz-2 in C57mg mammary epithelial cells and compared this cell line to C57mg cells over-expressing Wnt-1. (biomedcentral.com)
Different Wnts have distinct effects on gene expression in a single cell line. (biomedcentral.com)
Beta-catenin then accumulates in the nucleus where it interacts with its transcriptional co-activators, members of the Lymphoid Enhancer Factor/T Cell Factor (LEF/TCF) family of high mobility group DNA binding proteins to regulate gene expression [ 1 ]. (biomedcentral.com)
LPS effectively inhibited cell viability and LC3B I expression and enhanced LC3B II, Beclin-1 and atg5 expressions in MLE-12 cells. (karger.com)
3-methyladenine promoted cell viability and ZO-1 expression, down-regulated Beclin-1 and atg5 expression, while Rapamycin (Rap) had an opposite effect. (karger.com)
Astragaloside IV suppressed cell viability and ZO-1 expression after the Rap treatment. (karger.com)
It is expressed on pro-B, pre-B and mature B cells, but expression is strongly down-regulated after B cell activation. (stemcell.com)
showed significant upregulation of miR-301a in the heart of diabetic ventricles to regulate expression of voltage-gated potassium channel (Kv4.2) by direct interaction with 3′UTR in the diabetes mouse (db/db) model ( 9 ). (frontiersin.org)
C-BF also reduced LPS induced TNF-α expression through the NF-κB signaling pathway in mouse RAW 264.7 macrophages. (unboundmedicine.com)
Cells which lack ER expression at diagnosis arise from an ER compart- were harvested at 60 - 80% confluency and used as a reference in all hybrid- ment within the mammary epithelium or represent evolution from an izations. (lu.se)
We also investigated the role of CysLT1R in 1) FAP/sporadic colorectal cancer by crossing ApcMin/+ mice with mice lacking CysLT1R expression and in 2) colitis-associated colorectal cancer by employing the AOM/DSS-model on mice lacking CysLT1R expression. (lu.se)

Methods: Mouse mammary glands were dissociated and stained with CD24. (cardiff.ac.uk)
This laboratory manual provides cutting-edge methods for generating and characterizing mouse models that accurately recapitulate many features of human cancer. (cshlpress.com)

miR-301a is initially identified as a novel ncRNA to be upregulated in T-helper cells in response to myelin oligodendrocyte protein antigen in the multiple sclerosis ( 8 ). (frontiersin.org)

During postnatal nephrogenesis, Troy+ cells are present in the cortex and papilla and display an immature tubular phenotype. (knaw.nl)

These data demonstrate a critical role for DNMT3A in orchestrating intestinal epithelial homeostasis and response to tissue damage and suggest an involvement of impaired epithelial DNMT3A function in the etiology of IBD. (nature.com)
When intestinal cells or tissue samples collected from celiac disease patients were pre-incubated with VX-770 before being exposed to P31-43, the peptide did not elicit an immune reaction. (eurasiareview.com)
It is described as a housekeeping enzyme, being responsible for cell-to-cell signaling, tissue homeostasis, and cytoprotection. (medscape.com)

In the adult kidney, Troy+ cells are present in the papilla and these cells continue to contribute to collecting duct formation during homeostasis. (knaw.nl)
Dysregulation in intestinal epithelial monolayer cause abnormal interaction with immune cells and perturb the intestinal immune homeostasis which is associated with the clinical symptoms of IBD. (frontiersin.org)

Cell viability was estimated by cell counting kit-8 and cell apoptosis by flow cytometry. (karger.com)
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD24 Antibody, Clone M1/69, followed by a mouse anti-rat IgG2b antibody, FITC (filled histogram), or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530 (Catalog #60077), followed by a mouse anti-rat IgG2b antibody, FITC (solid line histogram). (stemcell.com)
Stains mouse Lgr5 transfected cells but not irrelevant transfectants in Flow Cytometry and Immunocytochemistry. (rndsystems.com)
Detection of Lgr5/GPR49 in HEK293 Human Cell Line Transfected with Mouse Lgr5/GPR49 and eGFP by Flow Cytometry. (rndsystems.com)

Genetic deficiency induces embryonic lethality in Dnmt1 and Dnmt3b-deleted mice and postnatal lethality in Dnmt3a-knockout mice at ~4 weeks of age 10 . (nature.com)
A dedifferentiation program was suggested for inducing differentiated somatic cells to become pluripotent embryonic-like stem cells (Halley-Stott et al. (forgetmenotinitiative.org)
HEK293 human embryonic kidney cell line transfected with mouse Lgr5/GPR49 and eGFP was stained with and either (A) Rat Anti-Mouse Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8240) or (B) Rat IgG 2B Isotype Control (Catalog # MAB0061 ) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113 ). (rndsystems.com)
Lgr5/GPR49 was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with GFP (green) tagged mouse LGR5 using Rat Anti-Mouse Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8240) at 10 µg/mL for 3 hours at room temperature. (rndsystems.com)
Rat Anti-Mouse Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8240) induces TOPflash activity in the HEK293 human embryonic kidney cell line stably expressing LGR5 in the presence of Wnt-3a, but in the absence of R-Spondins. (rndsystems.com)
2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)
Immunohisto- have been used for positive selection of NSCs from em- chemistry on human embryonic central nervous system bryonic mice (Nagato et al. (lu.se)

In summary, CD97 increases the structural integrity of enterocytic adherens junctions by increasing and stabilizing junctional beta-catenin, thereby regulating intestinal epithelial strength and attenuating experimental colitis. (nih.gov)

Since invasive pneumococcal strains adhere to, and translocate across, respiratory tract epithelial cells, adhesion is a prerequisite for establishing pneumococcal disease [ 4 ]. (ersjournals.com)
however, the selectivity for infection of specific respiratory cell populations is not well described, and any relationship between receptors in the mouse and human lungs is incompletely understood. (elsevierpure.com)

Tracing of Troy+ cells during nephrogenesis demonstrates that Troy+ cells clonally give rise to tubular structures that persist for up to 2 y after induction. (knaw.nl)
Kidney-specific knockout of Sav1 in the mouse promotes hyperproliferation of renal tubular epithelium through suppression of the Hippo pathway. (nih.gov)

E-cadherin is the epithelial cell ligand for αIELβ7 integrin. (bdbiosciences.com)

Astragaloside IV might suppress autophagy initiation directly or indirectly through suppressing the oxidative stress and inflammatory response, which further enhances the cell viability and tight junction and reduces apoptosis in LPS-stimulated pulmonary endothelial ARDS cell model, thus exerting its therapeutic function in ARDS. (karger.com)

Following exposure to pathogens, the innate immune cells of gastrointestinal mucosa release pro-inflammatory cytokines and activate an adaptive immune response to aggravating inflammation in the intestine ( 3 ). (frontiersin.org)

Results: Three populations of CD24 expressing cells were identified: CD24Negative, CD24Low and CD24High. (cardiff.ac.uk)
CD24 is differentially expressed during T and B cell development, and is not found on the majority of mature peripheral T cells or plasma cells. (stemcell.com)

The mammalian internal ear provides a limited capability in to regenerate locks cells (Forge et al. (forgetmenotinitiative.org)
As a result, harm to mammalian locks cells outcomes in irreversible and everlasting hearing reduction usually. (forgetmenotinitiative.org)

To date, this has been hampered by the lack of suitable markers to separate out the two epithelial layers, and to remove contaminating non-epithelial cells. (cardiff.ac.uk)
Staining of these cells with cytokeratin markers revealed that these populations correspond to non-epithelial, myoepithelial/basal and luminal epithelial cells, respectively. (cardiff.ac.uk)
CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
2000). In *Correspondence to: Perrine Barraud, Department of Veterinary Medi- contrast, several cell surface markers have been used to cine, Neurosciences, Madingley Road, Cambridge CB3 OES, United enrich for NSCs in the rodent CNS. (lu.se)

Control cells cultured for the same periods of time as MNU-treated cells produced only ductal outgrowths that were morphologically similar to those found in the mammary glands of adult virgin hosts. (eurekamag.com)
Methodology: A total of 28 adult BALB/c mice were randomly assigned into a control and an experimental group. (bvsalud.org)

Additionally, EpCAM has oncogenic potential via its capacity to upregulate c-myc, e-fabp, and cyclins A & E. Since EpCAM is expressed exclusively in epithelia and epithelial-derived neoplasms, EpCAM can be used as diagnostic marker for various cancers. (wikipedia.org)

A single exposure or multiple exposures of these cells to MNU was effective in inducing tumorigenic cells that produced palpable tumors as early as 6 weeks after transplantation. (eurekamag.com)
This theory holds that tumors arise from 1 of 2 undifferentiated stem cells: the excretory duct reserve cell or the intercalated duct reserve cell. (medscape.com)

Mammary epithelial cells, isolated from virgin BALB/c mice, were embedded within collagen gels and grown in a serum-free medium containing prolactin, progesterone, and linoleic acid. (eurekamag.com)

To investigate the function of CD97 in colorectal carcinogenesis, transgenic Tg(villin-CD97) mice overexpressing CD97 in enterocytes were generated and subjected to azoxymethane (AOM)/dextran sodium sulfate (DSS)-induced colitis-associated tumorigenesis. (nih.gov)
Unexpectedly, we found a CD97 cDNA copy number-dependent reduction of DSS-induced colitis in Tg compared to wild-type (WT) mice that was confirmed by applying a simple DSS protocol. (nih.gov)

Ultrastructural analysis revealed that overexpression of CD97 strengthened lateral cell-cell contacts between enterocytes, which, in contrast, were weakened in CD97 knockout (Ko) mice. (nih.gov)
Interferon / receptor knockout (Ifnar1-/-) mice were inoculated with recent USUV isolates from Africa and Europe, as well as the historic 1959 South African strain. (cdc.gov)
CysLT1R knockout female mice, but not male mice exhibited a reduced polyp formation in the small intestine and colon, respectively. (lu.se)

Important determinants for virus infection are epithelial cell receptors identified as glycans terminated by an α2,3-linked sialic acid (SA) that preferentially bind avian strains and glycans terminated by an α2,6-linked SA that bind human strains. (elsevierpure.com)
Viremia was highest in mice inoculated with the recent African strains and lowest in mice inoculated with the Netherlands strain. (cdc.gov)

Keratin also protects epithelial cells from damage or stress. (wikipedia.org)
Thus, VX-770 protects gluten-sensitive epithelial cells from the detrimental effect of gluten. (eurasiareview.com)
Our results indicate that exposure to an attenuated form of WNV protects against severe USUV disease in mice and elicits a neutralizing response to both WNV and USUV. (cdc.gov)

Transepithelial resistance was not altered in Tg and Ko mice, indicating that tight junctions were not affected. (nih.gov)

In Tg murine and normal human colonic enterocytes as well as in colorectal cell lines CD97 was localized preferentially in E-cadherin-based adherens junctions. (nih.gov)

Although DNMT3A and DNMT3B are highly homologous, their redundancy seems to be limited, and their unique biological function, e.g., specificity for methylation patterns in distinct cell types, is still elusive 9 . (nature.com)

Importance of the filamin A-Sav1 interaction in organ size control: evidence from transgenic mice. (nih.gov)

In LPS-induced ARDS cell model, astragaloside IV up-regulated cell viability, SOD activity and ZO-1 and LC3B I expressions but down-regulated cell apoptosis, TNF-α, IL-6, LC3B II, Beclin-1 and atg5 expressions and LDH and MDA levels. (karger.com)
found that FWGE can promote apoptosis in different types of cancer cell lines such as SNU-5, MKN-45, SNU-620, SNU-1, and SNU-16. (hindawi.com)
FWGE has significant antiproliferative effects and kills tumour cells by the induction of apoptosis via the caspase-poly (ADP-ribose) polymerase pathway [ 9 ]. (hindawi.com)

In latest years, regeneration of locks cells offers produced guaranteeing outcomes (Groves et al. (forgetmenotinitiative.org)

Limiting dilution analysis revealed that T cells reacting with the human histocompatibility leukocyte antigens (HLA) of the FLD were undetectable in the CD8+ T cell population and barely measurable in the CD4+ subset. (rupress.org)
Combined treatment of FWGE and 5-fluorouracil or dacarbazine resulted in a synergistic effect in the HCR-25 human cancer cell line and B16 murine melanoma cell line [ 6 ]. (hindawi.com)
Using human intestinal cell lines that are sensitive to gluten, the researchers found that one specific peptide, P31-43, directly binds to CFTR and impairs its function. (eurasiareview.com)
An appendix specifically addresses the use of mouse cancer models in translational studies and the integration of mouse and human clinical investigations. (cshlpress.com)
Lgr5/GPR49 in HEK293 Human Cell Line Transfected with Mouse Lgf5/GPR49. (rndsystems.com)

We then focused on two genes previously linked to various cancers, mesothelin and stromelysin-1, which are respectively up-regulated by Wnt-1 and Wnt-5a in C57mg cells. (biomedcentral.com)

Eleven to 14 days after the final carcinogen treatment, the cells were removed from the collagen gels and injected into the cleared mammary fat pads of syngeneic hosts to assay for transformed cell populations. (eurekamag.com)

Anatomy24

Organisms1

Diseases2

Chemicals and Drugs11

Analytical, Diagnostic and Therapeutic Techniques and Equipment4

Phenomena and Processes11

Disciplines and Occupations1

Information Science2

Tissues9

Thymic epithelial cells5

Airway11

Vitro5

Differentiation5

Progenitor9

Stem cell9

Lymphocytes3

Vivo3

Developmental Biology2

Biology5

Thymocytes2

Antibodies1

IECs3

Fermented Wheat G1

Progenitors4

Lung8

Pathways1

Organoids1

Isolate2

Fetal2

Cultures5

Mammary gland1

Dendritic cells1

Tumor7

Proliferation2

Disruption2

Susceptibility1

Expression17

Methods2

Protein1

Phenotype1

Tissue3

Homeostasis2

Flow cytometry4

Embryonic7

Experimental colitis1

Respiratory2

Tubular2

Ligand1

Endothelial1

Inflammation1

CD242

Mammalian2

Markers4

Adult2

Neoplasms1

Tumors2

BALB1

Colitis2

Knockout3

Strains2

Protects3

Tight junctions1

Adherens junctions1

Specificity1

Transgenic1

Apoptosis3

Regeneration1

Human5

Genes1

Assay1