Clonogenic assays were performed on leukemic and tumor cell lines to evaluate the in vitro antineoplastic activity of DAC. (biomedcentral.com)
Increasing the DAC concentration and duration of exposure produced a greater loss of clonogenicity for both human leukemic and tumor cell lines. (biomedcentral.com)
We have observed that DAC reduced the in vitro clonogenic potential in a dose-dependent manner of both human and murine leukemia and tumor cell lines. (biomedcentral.com)
But mice receiving EGME or EGEE and challenged with the same tumor cell concentration showed over 83 percent survival rates. (cdc.gov)
Survival time2
The in vivo antineoplastic activity of DAC administered as an i.v. infusion was evaluated in mice with murine L1210 leukemia by measurement of survival time, and in mice bearing murine EMT6 mammary tumor by excision of tumor after chemotherapy for an in vitro clonogenic assay. (biomedcentral.com)
EGME and EGEE treatment did not affect median survival time in syngeneic mice, indicating that these compounds did not inhibit tumor growth. (cdc.gov)
Leukemic2
Studies were conducted in the leukemic mouse and rat to test the hypothesis that enhanced effects of drugs given in sequence relate to a predictable increase in tumor growth and sensitivity to cycle-active agents. (johnshopkins.edu)
The reactivation of the TSGs ( p57KIP2 in HL-60 leukemic cells and p16CDKN2A in Calu-6 lung carcinoma cells) and the inhibition of global DNA methylation in HL-60 leukemic cells increased with DAC concentration. (biomedcentral.com)
Endothelial Cells1
2009) Synthesis of a Labeled RGD-Lipid, Its Incorporation into Liposomal Nanoparticles, and Their Trafficking in Cultured Endothelial Cells. (ubc.ca)
Tumors2
In mice with L1210 leukemia and in mice bearing EMT6 tumors, the antineoplastic action of DAC also increased with the dose. (biomedcentral.com)
M5076 tumors in C57BL/6 mice and to prolong survival of DBA/2 mice with disseminated L1210 leukemia. (unboundmedicine.com)
Concentration2
Interestingly, the half maximal inhibitory concentration (IC50) of BLMs in different cancer cell lines varies from nM to μM ranges. (lookformedical.com)
In recent studies, this linker modified analog of goniothalamin was shown to be cytotoxic against PC-3 (prostate cancer) and MCF-7 (human breast cancer) cell lines with half maximal inhibitory concentration values of 0.13 μM and 2.6 μM, respectively. (lookformedical.com)
Chemotherapy2
Phase II trial of induction chemotherapy of pemetrexed plus split-dose cisplatin followed by pemetrexed maintenance for untreated non-squamous non-small-cell lung cancer. (shengsci.com)
abstract： PURPOSE:We conducted a phase II trial to evaluate the efficacy and safety of induction chemotherapy of pemetrexed plus split-dose cisplatin followed by pemetrexed maintenance for advanced non-squamous non-small-cell lung cancer (NSCLC). (shengsci.com)
Antitumor2
The present study examined the role of CYP3A-mediated metabolism in the in vivo antitumor activity and host toxicity of MMDX in the mouse model and investigated the potential for increasing the therapeutic effectiveness of the drug by inducing its hepatic CYP-catalyzed activation. (unboundmedicine.com)
The role of autophagy in the death of L1210 leukemia cells initiated by the new antitumor agents, XK469 and SH80, Mol Cancer Ther 2007 Jan;6(1):370-9. (wayne.edu)
Smears2
Blood smears of allogeneic mice were made for differential counts on the last day of treatment, day of death, and 43 days post tumor transplantation. (cdc.gov)
the system is designed to identify and type hsv from direct lesion smears (ds) and from cell culture smears (cs) infected with a swab from the patient's lesion by means of an indirect fluorescence assay (ifa) using hsv type-common and hsv-2 type-specific monoclonal antibodies. (liverpool.ac.uk)
Concentrations1
We also observed a correlation between in vitro and in vivo concentrations needed to produce optimal responses in mice with L1210 leukemia or with EMT6 tumor. (biomedcentral.com)
Vitro3
The in vitro antineoplastic action of DAC correlated well with its inhibition of DNA methylation and reactivation of TSG in human neoplastic cells. (biomedcentral.com)
an enzyme linked immunosorbent assay (elisa) system to study specific anti-herpes simplex virus (hsv) antibody production in vitro by human peripheral blood mononuclear cells (pbmc) has been developed. (liverpool.ac.uk)
trifluorothymidine (tft) is known to be concentrated in herpes simplex virus (hsv) infected cells in vitro in the form of phosphorylated derivatives. (liverpool.ac.uk)
Anticancer3
Consequently, ROS-modulation has emerged as an anticancer strategy with synthesis of various ROS-inducing or responsive agents that target cancer cells. (degruyter.com)
abstract： PURPOSE:M30 and M65 ELISAs are proposed as surrogate biomarkers of tumour cell death in patients and are being applied increasingly in the pharmacodynamic (PD) evaluation of anticancer drugs during clinical trials. (shengsci.com)
Once inside, the anticancer activities rely on their abilities to produce DNA breaks, thus leading to cell death. (lookformedical.com)
Therapeutic1
This indicates the intricate control of metabolism and redox balance for survival of cancer cells that can be targeted by therapeutic agents. (degruyter.com)
Apoptosis2
Of note, an increased carbohydrate uptake and/or induction of death receptors of cancer cells was exploited to develop glycoconjugates that potentially induce cellular stress, ROS and apoptosis. (degruyter.com)
Chemistry of RNA and DNA base methylation The addition of the methyl-group to DNA and RNA bases (Fig.?2) is catalyzed by DNA- and RNA-methyltransferases that use DNMTs, which methylate canonical dC bases.26 In contrast, DNMT1 maintains the methylation status Actinomycin D biological activity during cell division. (thebiotechdictionary.com)
Receptors1
Both BLM and A5 are hydrophilic molecules that depend on transporters or endocytosis receptors to get inside of cells. (lookformedical.com)
Markedly2
We have shown that intensification of the DAC dose markedly increased its antineoplastic activity in mouse models of cancer. (biomedcentral.com)
Additional experiments carried out in healthy C57BL/6 mice showed that TAO markedly inhibited MMDX-induced myelosuppression and protected the animals against lethal doses of MMDX. (unboundmedicine.com)
Death3
Photodynamic therapy (PDT) is a form of phototherapy involving light and a photosensitizing chemical substance, used in conjunction with molecular oxygen to elicit cell death (phototoxicity). (wikipedia.org)
If sufficient oxidative damage ensues, this will result in target-cell death (only within the illuminated area). (wikipedia.org)
Qualification of M30 and M65 ELISAs as surrogate biomarkers of cell death: long term antigen stability in cancer patient plasma. (shengsci.com)
Compounds1
This mini review highlights the development of compounds that target cancer cells by taking advantage of redox or metabolic alteration in cancer cells. (degruyter.com)
Antigen1
this study compared conventional viral isolation (vi) in cell cultures with a commercial product--virgo antigen detection system--for the identification and typing of herpes simplex virus (hsv). (liverpool.ac.uk)
Reactive1
The reactive oxygen kills the target cells. (wikipedia.org)
Activity3
a complete loss of MMDX activity was observed in liver metastases-bearing animals receiving 40 microg/kg MMDX i.v. plus TAO. (unboundmedicine.com)
However, pregnenolone-16alpha-carbonitrile pretreatment failed to enhance MMDX activity in mice bearing either s.c. (unboundmedicine.com)
it was reported that glycoprotein c of hsv-1 (gc-1) mediates the binding of c3b-coated erythrocytes to infected cells and has regulatory (decay-accelerating) activity for the alternative pathway c3 convertase of human complement. (liverpool.ac.uk)
Time1
The longest survival (250% of controls) was in animals whose second course began on day 3, the time of peak HSA as measured by DNA synthesis induced in L-1210 cells in culture. (johnshopkins.edu)
Dose2
Preclinical efficacy evaluations of XK-469: dose schedule, route and cross-resistance behavior in tumor bearing mice. (wayne.edu)
The values of LD50 (lethal dose 50%) calculated from experiments are different for each cell line . (lookformedical.com)
Water3
Photosensitisers have been employed to sterilise blood plasma and water in order to remove blood-borne viruses and microbes and have been considered for agricultural uses, including herbicides and insecticides. (wikipedia.org)
All allogeneic mice challenged with 3,000,000 L1210 cells and receiving water or CP treatment died. (cdc.gov)
Although hydrazine sulfate is carcinogenic i.e., can cause cancer in some weanling mice given the drug in their drinking water since birth, there has never been a case of human cancer reported as a result of HS therapy . (hydrazinesulfate.org)
Human1
infection of human epidermoid carcinoma no. 2 cells with herpes simplex virus type 1 (hsv-1) leads to a reorganization of antigens associated with both the small and heterogeneous nuclear ribonucleoprotein complexes (snrnp and hnrnp). (liverpool.ac.uk)
Liver1
We found that MMDX cytotoxicity for cultured M5076 tumor cells was potentiated 22-fold by preincubating the drug with NADPH-supplemented liver microsomes from untreated C57BL/6 female mice. (unboundmedicine.com)
Blood1
Bilirubin is a chemical that is produced when old red blood cells break down. (webmd.com)
Cancer cell2
High abundance of glucose in the cytoplasm of a cancer cell also increases flux into other metabolic pathways such as hexosamine biosynthetic pathway (HBP) [ 7 ]. (degruyter.com)
ROS-modulation in normal vs. cancer cell. (degruyter.com)
Cellular1
Redirection of glutamine to cellular energetics destabilizes glutathione homeostasis in glioblastoma cells and other cancer cells [ 9 ]. (degruyter.com)
Enhance1
Cancer cells also enhance glucose-uptake to fuel these enhanced metabolic activities [ 2 ]. (degruyter.com)
Role2
The key role in the pathogenesis of sarcoidosis is played by proinflammatory cytokines and chemokines, molecules crucially involved in the activation of immune and inflammatory cells and their trafficking to the site of disease [4]. (thebiotechdictionary.com)
This study aimed to explore the role and mechanism of action of sulforaphane (SFN) in protecting mouse Leydigs (TM3) cells from cadmium (Cd)-induced damage. (lookformedical.com)
Greater1
using [4-14c]edu as the radiolabeled analogue of edu, it was ascertained that, at antivirally active doses, edu is phosphorylated to a much greater extent by hsv-infected vero cells than by mock-infected cells. (liverpool.ac.uk)
Expression1
2005). Investivgation of factors responsible for cell line cytoplasmic expression differences. (ubc.ca)
Treated with 1001
LBN rats bearing acute myelocytic leukemia (AML) were treated with 100 mg Ara-C/kg q.8 h six times begining on day 0 and on other days in sequence (0.1 through 0.12). (johnshopkins.edu)