• it is imperative to understand the implications of diet on epigenetic modifications, and the effect of those modifications on the development of cancer today and in future generations. (scentoferos.com)
  • DNA methylation is an inheritable chemical modification of cytosine, and represents one of the most important epigenetic events. (biomedcentral.com)
  • A major type of epigenetic event is DNA methylation, which involves the addition of a methyl group to the number 5 carbon of the cytosine pyrimidine ring [ 2 ]. (biomedcentral.com)
  • Notwithstanding rapid developments in epigenome-mapping methods that can explore a large number of chromatin modifications, the only approach that can screen thousands of samples needed to detect trans -acting relationships in a genome-wide fashion is an array-based multiplex assay that interrogates a multitude of methylation sites in parallel. (nature.com)
  • The phenotypic profiles of the cells, as well as the expression of histone demethylases, were studied through immunofluorescence, transient transfection, western blot, and qRT-PCR analysis after inducing hypertrophy by angiotensin II and endothelin-1. (hindawi.com)
  • Therefore, a balance of the methylation/demethylation of lysine residues in histones is important for gene expression and genomic integration [ 9 , 10 ]. (hindawi.com)
  • Both the localization of the lysine residues on histone tails and the degree of methylation (mono-, di-, or trimethylation) modify the state of differential gene expression [ 10 - 12 ]. (hindawi.com)
  • In the present work, the role of neurohormones ET-1 and Ang II in inducing cardiac hypertrophy was examined and the expression of some histone demethylases was evaluated. (hindawi.com)
  • Although cells contain a myriad of biomolecules that can potentially be assayed across large numbers of genetically characterized samples, analyses of nucleic elements such as transcript expression and CpG methylation are ideally suited for genome-wide comparisons with currently available technologies. (nature.com)