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  • Variability
  • So by placing scores as into ranges of methylation level kinda takes into account the posibile variability from the PCR reactions, the sequencing reaction and the way the automated sequencer basecalls, taking note that the chromatographs we routinely see from a sequencing reaction has been normalised and processed. (protocol-online.org)
  • question
  • Now my question is why clone in a bacteria with a methyltransferase when we're essentialy trying to rid our sequence of methylation. (protocol-online.org)
  • My second question is I have a range of multiple cell lines and my MSP is showing that most of my cells are methylated with only one of my cell lines showing partial methylation. (protocol-online.org)
  • role
  • Understanding what triggers hypermethylation of tumour suppressor genes in cancer cells is critical if we are to discern the role of methylation in the oncogenic process. (protocol-online.org)
  • random
  • Instead our results support a model that requires a combination of prior gene silencing and random 'seeds' of methylation to trigger hypermethylation of the GSTP1 gene in the prostate cancer cell. (protocol-online.org)
  • certain
  • 1) If you focus on individual CpG sites, you can get the percentage of clones showing methylation at a particular site for each sample, then apply Chi-square test, and finally you can say that certain treatment causes significant methylation (or demehtylation) at what CpG site compared to untreated. (protocol-online.org)
  • look
  • As for confirmation of your results, you could look for methylation sensitive restriction sites close to or within your region of interest (such as HpaII and MspI). (protocol-online.org)