Methylation early mammalian embryos. Medical search. Frequent questions

The two enrichment strategies allow probing of methyl-CpG protein interactions in early vertebrate oocytes and embryos. (biomedcentral.com)
We examine how epigenetic states are set up in oocytes - or egg cells - and influence gene expression in the embryo. (babraham.ac.uk)
We are also interested in how variations in DNA methylation come about in oocytes and whether we can use this variation as a marker for oocyte quality and embryo potential. (babraham.ac.uk)
Here, we have utilized single-cell methylome and transcriptome sequencing (scM&T-seq) to quantify both mRNA expression and DNA methylation in oocytes and a developmental series of human embryos at single-cell resolution. (babraham.ac.uk)
To identify H3K9 methylation and other meaningful biological changes in each mutant to explore the molecular functions of EHMT1 and EHMT2, we performed immunofluorescence imaging, multi-omics sequencing, and mass spectrometry (MS)-based proteome analyses in cKO oocytes. (babraham.ac.uk)
Furthermore, there were more significant changes in the transcriptome, DNA methylome, and proteome in cDKO than cKO oocytes, with transcriptional derepression leading to increased protein abundance and local changes in genic DNA methylation in cDKO oocytes. (babraham.ac.uk)
Here, we report live mammalian offspring derived from single unfertilized oocytes, which was achieved by targeted DNA methylation rewriting of seven imprinting control regions. (theuglyminute.com)
Therefore, we generated genome-wide methylation and transcriptome maps of size-selected, growing oocytes to capture the onset and progression of methylation. (biomedcentral.com)
Finally, we show that establishment of non-CpG methylation, which is prevalent in fully grown oocytes, and methylation over non-transcribed regions, are later events in oogenesis. (biomedcentral.com)
As a result, there is highly programmed methylation of a defined set of ~2000 CGIs in oocytes, mostly on account of their location within active transcription units. (biomedcentral.com)
Human DNMT1o mRNA is detectable in mature oocytes and early fertilized embryos but not in any somatic tissues analysed. (biomedcentral.com)
Sibling oocytes were randomly allocated after insemination to early cumulus cell removal at 6 hours (group I) and late cumulus cell removal at 16-18 hours (group II). (ecerm.org)

His early work led to the discovery that the molecular mechanism of genomic imprinting is based on DNA methylation. (wikipedia.org)
Reik W, Collick A, Norris ML, Barton SC, Surani MA (1987) Genomic imprinting determines methylation of parental alleles in transgenic mice. (wikipedia.org)
Genomic sites of DNA methylation can be bound by methyl-CpG-binding domain proteins (MBDs) and specific zinc finger proteins, which can recruit co-repressor complexes to silence transcription on targeted loci. (biomedcentral.com)
DNA methylation has been shown to be involved in numerous processes, including X-chromosome inactivation in mammals, retrotransposon silencing, genomic imprinting, carcinogenesis and the regulation of tissue specific gene expression during development. (edu.au)
We find that the fidelity of DNMT1-mediated maintenance methylation is directly related to the local density of DNA methylation, and for genomic regions that are lowly methylated, histone modifications can dramatically alter the maintenance methylation activity. (deylab.com)
The expression of a normal pattern of genomic imprinting in the mouse embryo depends on the activity of the DNA methyltransferase Dnmt1 [ 1 ]. (biomedcentral.com)
this might involve the replication, propagation or interpretation of early methylation patterns at selected loci (in particular imprinted genes) that are then subsequently able to escape the global genomic demethylation that occurs in later preimplantation development [ 8 ]. (biomedcentral.com)
Cytosine methylation of DNA involves a subset of genomic cytosines methylated at the C5 position in some species. (i-sis.org.uk)

We show that upon fertilization, both paternal and maternal genomes undergo active DNA demethylation, and genome-wide de novo DNA methylation is also initiated in the same period. (nature.com)
DNA methylation is a widespread epigenetic modification in vertebrate genomes. (biomedcentral.com)
DNA methylation is an epigenetic modification mostly associated with transcriptional repression in vertebrate genomes. (biomedcentral.com)
By comparing these signatures with early embryos that have undergone spontaneous cleavage-stage arrest, as determined by time-lapse imaging, we identify embryos that fail to appropriately activate their genomes or undergo epigenetic reprogramming. (babraham.ac.uk)
In the past, our research focused on viral genomes as models for DNA replication in mammalian cell nuclei. (nih.gov)
Nucleus transplantation experiments in mouse zygotes in the early 1980s confirmed that normal development requires the contribution of both the maternal and paternal genomes. (wikipedia.org)
The vast majority of mouse embryos derived from parthenogenesis (called parthenogenones, with two maternal or egg genomes) and androgenesis (called androgenones, with two paternal or sperm genomes) die at or before the blastocyst/implantation stage. (wikipedia.org)
Howlett S.K., Reik W., Methylation levels of maternal , and paternal genomes during preimplantation development. (biologie-journal.org)
Reduced representation bisulfite sequencing (RRBS) has been widely used to profile genome-scale DNA methylation in mammalian genomes. (biomedcentral.com)

Although imprinting accounts for a small proportion of mammalian genes they play an important role in embryogenesis particularly in the formation of visceral structures and the nervous system. (wikipedia.org)
Parthenogenetic/gynogenetic embryos have twice the normal expression level of maternally derived genes, and lack expression of paternally expressed genes, while the reverse is true for androgenetic embryos. (wikipedia.org)
Changes in genes and regulatory elements are associated with exceptional mammalian traits, such as hibernation, that could inform therapeutic development. (bvsalud.org)
Gene expression is tightly regulated via DNA methylation (5mC) and the aberrant expression of meiotic genes in mitotic cells via CpG promoter hypomethylation has been proposed to cause cancer. (edu.au)
Although more highly transcribed genes acquire methylation earlier, the major transitions in the oocyte transcriptome occur well before the de novo methylation phase, indicating that transcription is generally not rate limiting in conferring permissiveness to DNA methylation. (biomedcentral.com)
Mammalian parental imprinting represents an exquisite form of epigenetic control regulating the parent-specific monoallelic expression of genes in clusters. (nature.com)
Mammalian parental imprinting is a form of epigenetic regulation that causes genes to be expressed from only one chromosome homolog according to parent-of-origin 1 , 2 . (nature.com)
Methylation at CpG islands of mammalian miR-9 genes was assessed in fetal murine neural stem cells (mNSCs) by methylation-specific PCR, and miRNA processing assessed by qRT-PCR for pre-miR-9 transcripts. (blogspot.com)
We used six bulls and characterized the DNA methylation levels of KDM2A , KDM5A , KMT2D , and DOT1L genes. (animal-reproduction.org)
Capra E, Lazzari B, Turri F, Cremonesi P, Portela AMR, Ajmone-Marsan P, Stella A, Pizzi F. Epigenetic analysis of high and low motile sperm populations reveals methylation variation in satellite regions within the pericentromeric position and in genes functionally related to sperm DNA organization and maintenance in Bos taurus. (animal-reproduction.org)
The methylation patterns of the IGF2 and IGF2R genes in bovine spermatozoa are not affected by flow‐cytometric sex sorting. (animal-reproduction.org)
Devos J, Behrouzi A, Paradis F, Straathof C, Li C, Colazo M, Block H, Fitzsimmons C. Genetic potential for residual feed intake and diet fed during early- to mid-gestation influences post-natal DNA methylation of imprinted genes in muscle and liver tissues in beef cattle. (animal-reproduction.org)
Differential methylation of the two alleles is a hallmark of imprinted genes. (biomedcentral.com)
Soon after fertilization, the vast majority of methylcytosine in sperm is converted by the Tet3 enzyme to hydroxymethylcytosine that gets lost by dilution during replication, effectively erasing cytosine methylation patterns except for a subset that is maintained, including those of some imprinted genes. (i-sis.org.uk)
However, genes expressed early in development may preferentially retain histones. (i-sis.org.uk)
These results show that developmental genes are highly vulnerable to postnatal paternal folate deficiency, reflecting congenital disabilities, such as abnormal eyes and skull bones, in early embryos born to FD WT fathers. (molcells.org)

Furthermore, due to ethical and legal concerns, very limited techniques can be applied to human embryos to validate some of significant conclusions drawn from descriptive studies regarding human embryonic development. (nature.com)
Here, we present whole-genome chromatin immunoprecipitation followed by high-throughput DNA sequencing (ChIP-seq) analyses of a wide variety of histone markers in the brain, heart, and liver of early human embryos shortly after their formation. (researchgate.net)

Adenot P.G., Szollosi M.S., Geze M., Renard J.P., Debey P., Dynamics of paternal chromatin changes in live one-cell mouse embryo after natural fertilization. (biologie-journal.org)
Adenot P.G., Mercier Y., Renard J.P., Thompson E.M., Differential H4 acetylation of paternal, maternal chromatin precedes DNA replication, and differential transcriptional activity in pronuclei of 1-cell mouse embryos. (biologie-journal.org)
Cheng X., Blumenthal R.M., Coordinated chromatin control: structural and functional linkage of DNA and histone methylation. (biologie-journal.org)
Fulka H., St John J.C., Fulka J., Hozak P., Chromatin in early mammalian embryos: achieving the pluripotent state. (biologie-journal.org)
Giraldo A.M., Hylan D.A., Ballard C.B., Purpera M.N., Vaught T.D., Lynn J.W., Godke R.A., Bondioli K.R., Effect of epigenetic modifications of donor somatic cells on the subsequent chromatin remodeling of cloned bovine embryos. (biologie-journal.org)
Instead, CGI methylation timing negatively correlates with enrichment for histone 3 lysine 4 (H3K4) methylation and dependence on the H3K4 demethylases KDM1A and KDM1B, implicating chromatin remodelling as a major determinant of methylation timing. (biomedcentral.com)
These results do not support a major role for transcriptional transitions in the time of onset of DNA methylation in the oocyte, but suggest a model in which sequences least dependent on chromatin remodelling are the earliest to become permissive for methylation. (biomedcentral.com)
This mark could be in the form of methylation or perhaps some other epigenetic modification to the chromatin. (biomedcentral.com)
NcRNAs such as those involved in RNA interference can be independently inherited, and can also direct chromatin modification and DNA methylation see [7, 8] RNA Inheritance of Acquired Characters , and Nucleic Acid Invaders from Food Confirmed , SiS 63). (i-sis.org.uk)

Most importantly, our DNA methyltransferase loss-of-function analysis indicates that DNA methylation influences early monkey embryogenesis. (nature.com)
Although the genome-wide DNA demethylation is believed to be a hallmark of mammalian embryogenesis, previous study also indicated that the somatic form of dnmt1 ( dnmt1s ) is actually expressed at each stage of pre-implantation embryos and plays a role in the maintenance of DNA imprinting 8 . (nature.com)
However, probably due to technological limitations, no study has yet revealed genome-wide DNA remethylation during early embryogenesis. (nature.com)
Monkeys have served as one of the most valuable models for understanding DNA methylation dynamics during early embryogenesis in human due to their similarities in genetics and early embryonic development 17 , 18 . (nature.com)
restorative research provides evidence for natural rejuvenation processes occurring at the epigenetic level during mammalian embryogenesis. (epigenie.com)
For example, the rejuvenation of the germline in mammalian offspring removes any deleterious aging-associated changes passed on by parents and returns epigenetic aging to " ground zero " during early embryogenesis to prevent accumulated damage from affecting future generations. (epigenie.com)
Fascinatingly, the young whippersnappers from the Gladyshev lab now reveal some fascinating insights into the dynamics and the mechanisms involved in the rejuvenation of DNA methylation profiles during the very early stages of mammalian embryogenesis. (epigenie.com)
Overall, this exciting study describes the natural rejuvenation events that occur during early development and the onset of organismal aging during embryogenesis. (epigenie.com)
Using QCANet, we were able to extract several quantitative criteria of embryogenesis from 11 early mouse embryos. (nature.com)
However, due to the extensive epigenetic reprogramming that occurs during embryogenesis, we hypothesized that the different methylation regions (DMRs) identified in the blastocysts may not maintain in adulthood. (animal-reproduction.org)
These results suggest that embryo DMRs were reprogrammed during the final stages of de novo methylation during embryogenesis or later in development. (animal-reproduction.org)

We find that differentiated mammalian cells operate with high DNA methylation stability, consistent with earlier reports. (nottingham.ac.uk)

or Ph.D. positions in the field of epigenetics of mammalian develpment. (umontreal.ca)
DNA methylation additionally regulates the epigenetics course of that bypasses genetic coding in transmitting chosen traits to mammals' offspring. (rosa-eterna.com)
Epigenetics and stroke: role of DNA methylation and effect of aging onblood-brain barrier recovery. (diagenode.com)

CpG island methylation and histone modifications appear to play a role in the epigenetic regulation of CTA expression, however, very little is known about their functions in vivo. (edu.au)
I observed that histone modifications could potentially serve as an indicator of the methylation status of CTA gene promoters and that the expression of CTAs was inversely related to gene promoter 5mC levels. (edu.au)
Huang J.C., Lei Z.L., Shi L.H., Miao Y.L., Yang J.W., Ouyang Y.C., Sun Q.Y., Chen D.Y., Comparison of histone modifications in in vivo and in vitro fertilization mouse embryos. (biologie-journal.org)
Overall, our results demonstrate that while distinct cell states can substantially impact the genome-wide activity of the DNA methylation maintenance machinery, locally there exists an intrinsic relationship between DNA methylation density, histone modifications and DNMT1-mediated maintenance methylation fidelity that is independent of cell state. (deylab.com)
Small RNAs including short-interfering (si)RNAs and piRNAs, and long RNAs such as long intergenic noncoding (linc)RNAs can direct histone modifications and cytosine methylation. (i-sis.org.uk)

To understand how cell state transitions impact DNMT1-mediated maintenance methylation, we scaled the method down and combined it with the measurement of mRNA to simultaneously quantify genome-wide methylation levels, maintenance methylation fidelity and the transcriptome from the same cell (scDyad&T-seq). (deylab.com)
Applying scDyad&T-seq to mouse embryonic stem cells transitioning from serum to 2i conditions, we observe dramatic and heterogenous demethylation and the emergence of transcriptionally distinct subpopulations that are closely linked to the cell-to-cell variability in loss of DNMT1-mediated maintenance methylation activity, with regions of the genome that escape 5mC reprogramming retaining high levels of maintenance methylation fidelity. (deylab.com)
We are now elucidating novel molecules that function upstream of NP95-DNMT1, and couple DNA replication with maintenance methylation in mammalian cells. (riken.jp)

Here, we present a new conceptual framework, Ratio of Concordance Preference (RCP), that uses double-stranded methylation data to quantify the flexibility and stability of the system that gave rise to a given set of patterns. (nottingham.ac.uk)
Couldrey C., Lee R.S., DNA methylation patterns in tissues from mid-gestation bovine foetuses produced by somatic cell nuclear transfer show subtle abnormalities in nuclear reprogramming. (biologie-journal.org)
Because Dnmt1 shows preferential activity against hemimethylated DNA, it has been proposed to be involved primarily in maintaining methylation patterns through rounds of DNA replication, rather than establishing them de novo . (biomedcentral.com)
Differential methylation patterns in lean and obese non-alcoholicsteatohepatitis-associated hepatocellular carcinoma. (diagenode.com)
Folate-deficient postnatal diet can change H3K4me3 patterns in sperm that are not erased after fertilization, but persist throughout preimplantation embryos. (molcells.org)
Changes in H3K4me3 patterns are linked to dysregulated gene expression in early development, leading to congenital disabilities in the offspring. (molcells.org)

We also identified differential enrichment of transcription factor binding motifs in CGIs acquiring methylation early or late in oocyte growth. (biomedcentral.com)
Imprints entail the maintenance of germline-derived differential epigenetic marks, mostly in the form of DNA methylation, through to the somatic cells of the offspring. (nature.com)

Genome-wide assessment of DNA methylation alterations induced by superovulation, sexual immaturity and in vitro follicle growth in mouse blastocysts. (babraham.ac.uk)
To achieve this, cells of in vitro produced bovine embryos were traced from the 2-cell stage to the blastocyst stage. (bioone.org)
Potential projects center around the transmission and maintenance of maternal heterochromatin in the murine embryo or around the epigenetic impact of in vitro production of bovine embryos. (umontreal.ca)
Combining in vivo and in vitro assays, we show that the proliferation and differentiation capabilities of NSPCs decrease significantly in Ythdf2 −/− embryos. (biomedcentral.com)
In conventional in vitro fertilization (IVF), co-incubation of the cumulus-oocyte complex and sperm for 16-18 hours can imitate natural conditions, but prolonged exposure time may generate high levels of reactive oxygen species produced by cumulus cells and excess spermatozoa, resulting in impaired embryo development and vitality [ 1 , 2 ]. (ecerm.org)

Broader application of our RCP framework will permit comparison of epigenetic-information systems across cells, developmental stages, and organisms whose methylation machineries differ substantially or are not yet well understood. (nottingham.ac.uk)
During pre-implantation stages of mammalian development, maternally stored material promotes both the erasure of the sperm and oocyte epigenetic profiles and is responsible for concomitant genome activation. (babraham.ac.uk)
In the rare instances that they develop to postimplantation stages, gynogenetic embryos show better embryonic development relative to placental development, while for androgenones, the reverse is true. (wikipedia.org)
We show that the conditional depletion of the m 6 A reader protein Ythdf2 in mice causes lethality at late embryonic developmental stages, with embryos characterized by compromised neural development. (biomedcentral.com)
Thus, investigating methylation at intermediate stages would be informative, but genome-wide studies have not yet been done. (biomedcentral.com)
Notably, this region included a Zfp57 binding site that is essential for maintaining paternal methylation during the early stages of pre-implantation 5 , 6 . (nature.com)
Revealing cell populations catching the early stages of the human embryo development in naïve pluripotent stem cells. (axonmedchem.com)

By the 8-cell stage, remethylation becomes more pronounced than demethylation, resulting in an increase in global DNA methylation. (nature.com)
Abdalla H., Yoshizawa Y., Hochi S., Active demethylation of paternal genome in mammalian zygotes. (biologie-journal.org)
Oocyte coinjection of catalytically inactive Cas9 (dCas9)-Dnmt3a or dCpf1-Tet1 messenger RNA (mRNA) with single-guide RNAs (sgRNAs) targeting specific regions induced de novo methylation or demethylation, respectively, of the targeted region. (theuglyminute.com)
Further, to gain deeper insights into the methylation and demethylation turnover dynamics, we extended Dyad-seq to quantify all combinations of 5mC and 5-hydroxymethylcytosine (5hmC) at individual CpG dyads to show that TET proteins preferentially hydroxymethylate only one of the two 5mC sites in a symmetrically methylated CpG dyad rather than sequentially convert both 5mC to 5hmC. (deylab.com)
Our current studies examine the nuanced and long-term developmental impact of DNA demethylation mediated by TET1, as well as its non-catalytic functions, during early embryonic differentiation using mouse models. (bcm.edu)

Here we report genome-wide composition, patterning, and stage-specific dynamics of DNA methylation in pre-implantation rhesus monkey embryos as well as male and female gametes studied using an optimized tagmentation-based whole-genome bisulfite sequencing method. (nature.com)
Our study provides the first comprehensive illustration of the 'wax and wane' phases of DNA methylation dynamics. (nature.com)
We therefore decided to investigate comprehensively the global and high-resolution DNA methylation dynamics during early development of a non-human primate (rhesus monkey, Macaca mulatta ). (nature.com)
The first of our time-traveling studies in this rejuvenation face-off takes inspiration from nature and studies the dynamics and mechanisms associated with the rejuvenation of DNA methylation profiles in the cells of the early mammalian embryo. (epigenie.com)
My graduate and postdoctoral research has focused on investigating how the genome and epigenome regulates the dynamics of gene expression in viral and mammalian systems 8 . (confex.com)
Methylome dynamics of bovine gametes and in vivo early embryos. (animal-reproduction.org)
Altered DNA methylation dynamics are a conserved aspect of biological ageing and ha. (diagenode.com)

However, this resulted in only very subtle DNA methylation changes, and it remains unknown whether these alterations affect the expression of IAP elements and other ERVs. (biomedcentral.com)
Alterations in DNA methylation corresponding with lung inflammation and as a biomarker for disease development after MWCNT exposure. (sciendo.com)
Transcriptional alterations in glioma result primarily from DNA methylation-independent mechanisms. (igred.fr)

DNA cytosine methylation is an important epigenetic modification that plays a key role in gene expression. (edu.au)
though major model organisms such as worms and flies have perfectly functional epigenetic inheritance despite lacking cytosine methylation. (i-sis.org.uk)
Conversely, maternal cytosine methylation is protected from hydroxylation by the PGC7/Dppa3/Stella protein and methylation pattern is effectively maintained. (i-sis.org.uk)

My laboratory has developed new technologies and applied them towards understanding the molecular biology and enzymology of DNA replication in animal cells and viruses (SV40, polyomavirus, papillomavirus, and herpes simplex virus), and at the beginning of animal development (mouse preimplantation embryos and frog eggs). (nih.gov)
Moreover, previous studies have been performed in primed ESCs, which are not epigenetically or transcriptionally representative of preimplantation embryos. (biomedcentral.com)

He discovered some of the key epigenetic mechanisms important for mammalian development, physiology, genome reprogramming, and human diseases. (wikipedia.org)
It will be of great importance to define proteins recruited to methylated DNA during early development. (biomedcentral.com)
2) What are the requirements for DNA replication and transcription at the beginning of mammalian development? (nih.gov)
That imprinting might be a feature of mammalian development was suggested in breeding experiments in mice carrying reciprocal chromosomal translocations. (wikipedia.org)
The first lineage specification during mammalian embryo development can be visually distinguished at the blastocyst stage. (bioone.org)
Christians E., Rao V.H., Renard J.P., Sequential acquisition of transcriptional control during early embryonic development in the rabbit. (biologie-journal.org)
My research interests focus on the process of epigenetic remodeling during germline and early embryonic development. (umontreal.ca)
Following parthenogenetic activation, these edited regions showed maintenance of methylation as naturally established regions during early preimplantation development. (theuglyminute.com)
The transfer of modified parthenogenetic embryos into foster mothers resulted in significantly extended development and finally in the generation of viable full-term offspring. (theuglyminute.com)
Thus, embryo development is highly dynamic. (nature.com)
Zebrafish embryos were exposed to EtOH during gastrulation, or injected with anti-miR-9 or nonsense control morpholinos during the 2-cell stage of development and collected between 24 and 72 hours postfertilization (hpf). (blogspot.com)
In the last four decades, transgenic and knockout mouse models have helped to understand the mechanisms of mammalian sex determination, germ cell development, and adult gonad functions. (lidsen.com)
Transmission of 5-methylcytosine (5mC) from one cell generation to the next plays a key role in regulating cellular identity in mammalian development and diseases. (deylab.com)
Therefore, due to the highly dynamic epigenetic state during early embryonic development, we suggest that is essential to validate the DMRs found in embryos in adult individuals. (animal-reproduction.org)
Canovas S, Ross PJ, Kelsey G, Coy P. DNA Methylation in Embryo Development: Epigenetic Impact of ART (Assisted Reproductive Technologies). (animal-reproduction.org)
Davis TL, Yang GJ, McCarrey JR, Bartolomei MS. The H19 methylation imprint is erased and re‐established differentially on the parental alleles during male germ cell development. (animal-reproduction.org)
Dean W, Santos F, Stojkovic M, Zakhartchenko V, Walter J, Wolf J, Reik W. Conservation of methylation reprogramming in mammalian development: aberrant reprogramming in cloned embryos. (animal-reproduction.org)
Choline acts during preimplantation development of the bovine embryo to program postnatal growth and alter muscle DNA methylation. (animal-reproduction.org)
The impact of early mechanical removal of cumulus cells on fertilization and embryonic development is not yet precisely known. (ecerm.org)
This study aimed to investigate the effects of early and late cumulus cell removal on fertilization, polyspermy, embryonic development potential, blastocyst development, and clinical outcomes. (ecerm.org)
Early cumulus cell removal at 6 hours had no significant difference in fertilization, polyspermy, embryo development, or obstetric and perinatal outcomes compared to late removal. (ecerm.org)
They can also provide nutrients, hormones, and glycosaminoglycans, which are essential for the growth and development of embryos. (ecerm.org)
Development of DNA methylation-based epigenetic age predictors inloblolly pine (Pinus taeda). (diagenode.com)
The Developmental Genetics Research Group studies epigenetic regulation of organ development and stem cell functions, mediated by Polycomb group (PcG) proteins and DNA methylation (5mC) mechanisms. (riken.jp)

For time-lapse observation of early-stage D rosophila embryos, Keller et al. (nature.com)

Our current research now focuses on two basic, interrelated questions: (1) How do mammalian cells decide where and when to initiate DNA replication? (nih.gov)
Upon senescence, Jmjd3 is overexpressed and the MLL1 protein is recruited to the locus provoking the dissociation of Polycomb from the INK4/ARF locus, its transcriptional activation and its replication during early S-phase. (plos.org)

This work provides the useful knowledge on designing and executing RRBS for investigating the genome-wide DNA methylation in tissues from pigs. (biomedcentral.com)

DNA CpG methylation on the cytosine is among the most stable forms of epigenetic mechanisms in the life cycle of mammals. (nature.com)
The age-old battle of nature versus nurture has entered new frontiers with two exciting studies exploring different mechanisms that drive "epigenetic rejuvenation" - also known as the science of how our old and creaky DNA methylation-based epigenetic clocks can be wound back into a frolicking and fancy-free state. (epigenie.com)
therefore, it provides a unique opportunity to investigate the extent to which different sequence features acquire methylation as a result of common or distinct mechanisms. (biomedcentral.com)

He found that the environment influences epigenetic programming in embryos, with changes in gene expression persisting in adults and their offspring. (wikipedia.org)
EtOH also disrupts the relationship between miR-9 and target gene expression, suggesting a nuanced relationship between EtOH and miRNA regulatory networks in the developing embryo. (blogspot.com)

We fully characterize embryonic genome activation and maternal transcript degradation and map key epigenetic reprogramming events in developmentally high-quality embryos. (babraham.ac.uk)
However, in 2004, experimental manipulation by Japanese researchers of a paternal methylation imprint controlling the Igf2 gene led to the birth of a mouse (named Kaguya) with two maternal sets of chromosomes, though it is not a true parthenogenone since cells from two different female mice were used. (wikipedia.org)
Haaf T., The battle of the sexes after fertilization: behaviour of paternal and maternal chromosomes in the early mammalian embryo. (biologie-journal.org)
Associations of maternal diet and placenta leptin methylation. (animal-reproduction.org)

however, little is known about the kinetics of methylation gain genome wide and the reasons for asynchrony in methylation at imprinted loci. (biomedcentral.com)

DNA methylation (or 5-methylcytosine (5mC)) plays an essential role in ERV repression in postimplantation embryos and male germ cells [ 6 , 7 ]. (biomedcentral.com)

No single solution of RRBS is optimal in all circumstances for investigating genome-scale DNA methylation. (biomedcentral.com)

Methylation defects have also been associated with male infertility. (wikipedia.org)
Early life exposures to environmental insults have a profound impact on health across the course of life, ranging from birth defects to increased susceptibility to diseases such as cancer, cardiovascular and metabolic disease in adulthood. (bcm.edu)
Paternal folate deficiency also worsens H3K4me3 alteration in TG sperm, and FD TG fathers' embryos show severer defects. (molcells.org)
DNA methylation profiling reveals a pathological signature that contributes to transcriptional defects of CD34(+) CD15(-) cells in early chronic-phase chronic myeloid leukemia. (igred.fr)

In the female germline, DNA methylation is acquired late in oogenesis from an essentially unmethylated baseline and is established largely as a consequence of transcription events. (biomedcentral.com)

Kopecny V., Fléchon J.E., Camous S., Fulka J. Jr., Nucleologenesis , and the onset of transcription in the eight-cell bovine embryo: fine-structural autoradiographic study. (biologie-journal.org)

H3K9me2 was found at several paternally methylated imprinted regions in sperm, suggesting that this histone mark signals special sites of the paternal genome where methylation is maintained. (i-sis.org.uk)

Later, when these mice mature, they bred these male mice with WT female mice fed with a regular chow diet to examine pregnancy outcomes, H3K4me3 histone methylation in sperm and eight-cell embryos, and transcriptome of early embryos. (molcells.org)
PCA of eight-cell embryo transcriptome revealed evident clustering based on paternal diet and genotype. (molcells.org)

Here we describe Dyad-seq, a method that combines enzymatic detection of modified cytosines with nucleobase conversion techniques to quantify the genome-wide methylation status of cytosines at the resolution of individual CpG dinucleotides. (deylab.com)

Endogenous MeCP2 and MBD3 were precipitated from Xenopus oocyte extracts and conditions for methylation-specific binding were optimized. (biomedcentral.com)
For a reverse experiment, DNA methylation in early Xenopus embryos was assessed by MBD affinity capture. (biomedcentral.com)

We conclude that preference for concordant DNA methylation is a consistent mode of information transfer, and thus provides epigenetic stability across cell divisions, even in stem cells and those undergoing developmental transitions. (nottingham.ac.uk)
Failure of mammalian cells to regulate their proliferation cycle leads to cancer. (nih.gov)
In mouse embryos, cells seem prepatterned to become certain cell lineage because the first cleavage plane has been related with further embryonic-abembryonic axis at the blastocyst stage. (bioone.org)
Surprisingly, we found that there is a significant percentage of the embryos (∼60%) with labeled and nonlabeled cells randomly distributed and intermingled. (bioone.org)
The H3K9 histone methyltransferase SETDB1 is essential for ERV repression in embryonic stem cells (ESCs), with DNA methylation also playing an important role. (biomedcentral.com)
Indeed, ERV silencing in embryonic stem cells (ESCs) is largely dependent on post-translational modification of histones, in particular methylation at H3K9. (biomedcentral.com)
However underneath regular situations, DNA methylation, catalyzed by Dnmt3b, performs an vital function in how younger, unformed mammalian cells divide and turn into extra specialised cells. (rosa-eterna.com)
Stem cells, discovered solely in early-stage embryos, can turn into some other sort of cell discovered within the physique. (rosa-eterna.com)
Because the cells in embryos are considerably crowded, an algorithm to segment individual cells in detail and accurately is needed. (nature.com)
In early embryos, cells are loosely connected to each other. (nature.com)
At the 8-cell stage, the embryo becomes compact, and the cells form a spherical mass called a morula. (nature.com)
In htz1Delta cells, Sir2 and Sir3 spread into flanking euchromatic regions, producing changes in histone H4 acetylation and H3 4-methylation indicative of ectopic heterochromatin formation. (sdbonline.org)
Efficient generation of embryonic stem cells from single blastomeres of cryopreserved mouse embryos in the presence of signalling modulators. (axonmedchem.com)
Silver, gold, and iron oxide nanoparticles alter miRNA expression but do not affect DNA methylation in HepG2 cells. (sciendo.com)
Carbon nanotube- and asbestos-induced DNA and RNA methylation changes in bronchial epithelial cells. (sciendo.com)
Sperm DNA methylation is predominantly stable in mice offspring bornafter transplantation of long-term cultured spermatogonial stem cells. (diagenode.com)
DNA methylation changes from primary cultures through senescence-bypassin Syrian hamster fetal cells initially exposed to benzo[a]pyrene. (diagenode.com)

Here, we aimed to characterize DMRs previously identified in embryos, in the blood and sperm of adult progenies of two groups of heifers (low S/Co and control). (animal-reproduction.org)
If total fertilization failure (TFF) was determined to have occurred at early cumulus cell removal, rescue intracytoplasmic sperm injection (ICSI) was performed. (ecerm.org)
In current practice, short co-incubation of the cumulus-oocyte complex with sperm and early cumulus cell removal (6 hours) is challenging [ 4 - 7 ]. (ecerm.org)
Subsequently, they found that insecticides DDT and permethrin, jet fuel, plastic additives phthalates and bisphenol A, and dioxin can all trigger trans-generational health effects in rats such as obesity and ovarian disease, and each resulted in a different pattern of methylation in sperm DNA, according to Skinner. (i-sis.org.uk)
Postnatal paternal folate deficiency increases congenital disabilities through H3K4me3 histone methylation changes in sperm and embryos. (molcells.org)
showed that a folate-deficient diet interfering with adequate methyl supply changes H3K4me3 histone methylation in sperm. (molcells.org)

Fertilization, polyspermy, embryo quality, and pregnancy outcomes were not significantly different between both groups. (ecerm.org)

Here, we investigated the role of EHMT1 in the oocyte in comparison to EHMT2 using oocyte-specific conditional knockout mouse models ( cKO, cKO, cDKO), with ablation from the early phase of oocyte growth. (babraham.ac.uk)
Aoki F., Worrad D.M., Schultz R.M., Regulation of transcriptional activity during the first , and second cell cycles in the preimplantation mouse embryo. (biologie-journal.org)
Arney K.L., Bao S., Bannister A.J., Kouzarides T., Surani M.A., Histone methylation defines epigenetic asymmetry in the mouse zygote. (biologie-journal.org)
Bouniol C., Nguyen E., Debey P., Endogenous transcription occurs at the 1-cell stage in the mouse embryo. (biologie-journal.org)
We showed that QCANet can be applied not only to developing mouse embryos but also to developing embryos of two other model species. (nature.com)

Given the predominant role of transcription, we sought to investigate whether transcription timing is rate limiting for de novo methylation and determines the asynchrony of methylation events. (biomedcentral.com)
Mammalian forms of the transcription repressor, Kaiso, can reportedly bind methylated DNA and non-methylated CTGCNA motifs. (biologists.com)

Surprisingly, TET2 does not drive changes in DNA methylation levels at IAPs, suggesting that it regulates these retrotransposons indirectly. (biomedcentral.com)

The group studies a population of coupled genetic oscillators in the vertebrate embryo termed the segmentation clock. (ens-lyon.fr)

Anatomy28

Organisms8

Diseases1

Chemicals and Drugs34

Analytical, Diagnostic and Therapeutic Techniques and Equipment15

Phenomena and Processes36

Disciplines and Occupations2

Information Science3

Oocytes12

Genomic8

Genomes9

Genes16

Human embryos2

Chromatin9

Embryogenesis11

Differentiated mammalian cells1

Epigenetics3

Histone modifications5

Maintenance methylation3

Patterns6

Differential2

Vitro5

Stages7

Demethylation5

Dynamics7

Alterations3

Cytosine methylation3

Preimplantation embryos2

Development24

Time-lapse1

Replication2

Tissues1

Mechanisms3

Gene expression2

Maternal4

Loci1

Germ cells1

Genome-scale1

Defects4

Germline1

Bovine embryo1

Paternal genome1

Transcriptome2

Quantify1

Xenopus2

Cells17

Sperm6

Pregnancy outcomes1

Mouse5

Transcription2

Regulates1

Vertebrate1