Methylation at promoter. Medical search. Frequent questions

This epigenetic modification is usually grouped in regions denominated CpG islands, which frequently co-localize with gene promoters, silencing the transcription of those genes. (cun.es)
We also analyze the contribution of enhancer DNA methylation on the expression of relevant genes in acute myeloid leukemia and chronic myeloproliferative neoplasms. (cun.es)
Methylation profile of the promoter CpG islands of 14 "drug-resistance" genes in hepatocellular carcinoma. (wjgnet.com)
To establish the DNA methylation patterns of the promoter CpG islands of 14 "drug-resistance" genes in hepatocellular carcinoma (HCC). (wjgnet.com)
The methylation specific polymerase chain reaction in conjunction with sequencing verification was used to establish the methylation patterns of the 14 genes in the liver tissues of four healthy liver donors, as well as tumor and the paired non-cancerous tissues of 30 HCC patients. (wjgnet.com)
By crossreferencing methylation patterns to genome-wide mapping of histone H3 lysine (K) 4/27 trimethylation and binding of Oct4, Nanog, and Polycomb proteins on gene promoters, we found that promoter DNA methylation is the only marker of this group present on approximately 30% of genes, many of which are silenced in mES cells. (ca.gov)
Epigenetic aberrations of tumor suppressor genes (TSGs), particularly DNA methylation, are frequently involved in the pathogenesis of gastric cancer (GC). (jcancer.org)
Here, effects of changes in methylation status of 5-methyl cytosine in melatonin and female hormone receptor genes were investigated as possible mechanisms for increased breast cancer risk in female night shift workers. (fortunejournals.com)
Methylation in promoter regions of the MTNR1A, MTNR1B, PGR, ESR1 and ESR2 genes was analyzed by pyrosequencing in a nested case-control study of female nurses, including 354 breast cancer cases and 356 healthy controls. (fortunejournals.com)
No association between promoter methylation levels and breast cancer risk was observed for the other receptor genes investigated. (fortunejournals.com)
Furthermore, the fresh new methylation level of NLRC5 is additionally adversely coordinated to your term from NLRC5-situated family genes on the MHC category I pathway, in addition to HLA-A, HLA-B, HLA-C, B2M, LMP2, LMP7 and you can TAP1. (masazgorlice.pl)
Global transcriptome assays of both types of cells with or without 5-azacytidine treatment identified 640 genes, whose expression might be methylation-dependently down-regulated in the MG cells. (oncotarget.com)
Global methylation analysis revealed that 35 out of the 640 genes were hyper-methylated in the MG cells. (oncotarget.com)
We investigated whether DNA methylation of 5 tumor suppressor genes in pleural fluid samples could aid in diagnosis of malignant effusion. (elsevierpure.com)
In samples from 47 patients with malignant pleural effusions and 34 with nonmalignant effusions, we used a methylation-specific polymerase chain reaction to detect aberrant hypermethylation of the promoters of the DNA repair gene O 6 -methylguanine-DNA methyltransferase (MGMT), p16 INK4a , ras association domain family 1A (RASSF1A), apoptosis-related genes, death-associated protein kinase (DAPK), and retinoic acid receptor β (RARβ). (elsevierpure.com)
Sensitivity, specificity, and positive predictive value of methylation in one or more genes for diagnosis of malignant effusion were 59.6%, 79.4%, and 80.0% respectively. (elsevierpure.com)
In conclusion, aberrant promoter methylation of tumor suppressor genes in pleural fluid DNA could be a valuable diagnostic marker for malignant pleural effusion. (elsevierpure.com)
Promoters are located near the transcription start sites of genes, upstream on the DNA (towards the 5' region of the sense strand). (wikipedia.org)
Some cancer genes are silenced by mutation, but most are silenced by DNA methylation. (wikipedia.org)
In the cell nucleus, it seems that promoters are distributed preferentially at the edge of the chromosomal territories, likely for the co-expression of genes on different chromosomes. (wikipedia.org)
Since E1A and E1B jointly had a more pronounced effect, it was conceivable that genes in both regions acted together in the abrogation of the inhibitory effect of promoter methylations in the late E2A promoter. (uni-bielefeld.de)
Effects of promoter methylation on increased expression of polyamine biosynthetic genes in suicide. (ulaval.ca)
The MHC class II transactivator (MHC2TA) is the master controller of expression of class II genes, and methylation of the promoter of this gene has been previously been shown to alter its function. (ox.ac.uk)
Regional promoter CpG methylation with genome-wide hypomethylation jointly, as a simple epigenetic hallmark of malignancies, result in the silencing of tumor suppressor genes (TSG) and activation of oncogenes, adding to cancers development and initiation. (antiviralbiologic.com)
Recently, several whole-genome sequencing research of practically all individual malignancies also demonstrate the fact that mostly mutated genes are epigenetic modifiers including CpG methylation equipment components across different malignancies2,3,4,5, highlighting the immediate and essential participation of epigenetic development dysregulation in tumorigenesis. (antiviralbiologic.com)
The aim of this study was to identify genes in adenoid cystic carcinoma (ACC) of salivary gland strongly deregulated by epigenetic CpG island methylation, to validate selected genes by conventional techniques, and to correlate the findings with clinicopathologic factors. (nih.gov)
Aberrant promoter methylation of tumor suppressor genes has not been fully investigated in pediatric tumors. (elsevierpure.com)
Therefore, we examined the methylation status of nine genes (p16 INK4A , MGMT, GSTP1, RASSF1A, APC, DAPK, RARβ, CDH1 and CDH13) in 175 primary pediatric tumors and 23 tumor cell lines using methylation-specific PCR. (elsevierpure.com)
A cross-sectional study was designed to investigate the promoter methylation status of candidate genes in DI-exposed workers. (cdc.gov)
We studied four candidate genes (GSTM1, DUSP22, IFN-y, and IL-4) for which altered promoter methylation has been previously investigated for relationships with a variety of other environmental exposures. (cdc.gov)
Our finding suggests that exposure to occupational chemicals could play a heretofore undefined mechanistic role via epigenetic modification of specific genes in the promoter region. (cdc.gov)
DNA methylation is a chemical process that regulates the function of the genes. (lu.se)

Here, we examined the methylation status of IGFBP-3 to correlate to clinicopathological factors in human cancers. (nih.gov)

We report here genome-wide mapping of DNA methylation patterns at proximal promoter regions in mouse embryonic stem (mES) cells. (ca.gov)
We define choice promoters for the gene, a distal and proximal promoter. (accessibletech4all.org)
Although both promoters are energetic, just the proximal promoter was responsive and regulated simply by TGF-1 and constitutively active ALK5 adversely. (accessibletech4all.org)
Taken jointly, these research define TGF-1-mediated downregulation of TRIII mRNA appearance through effects in the ALK5/Smad2/3 pathway in the gene proximal promoter being a potential system for reduced TRIII appearance in individual malignancies. (accessibletech4all.org)
Taken together, our results suggest that hyper-methylation of the ZNF350 proximal promoter may be one of the crucial determinants for acquiring increased migratory capabilities in colon cancer cells. (oncotarget.com)
Bisulfite-treated DNA from leukocytes was evaluated for methylation levels in six CpG sites of the proximal promoter of the PPARGC1A gene by pyrosequencing (positions -816, -783, -652, -617, -521 and -515). (unav.edu)

The association between promoter methylation and familial breast cancer was tested in 40 unaffected premenopausal women in our cohort who underwent BRCA1/2 mutation testing. (umn.edu)

The characterization of the aberrant enhancer DNA methylation provides not only a novel pathogenic mechanism for different tumors but also highlights novel potential therapeutic targets for myeloid derived neoplasms. (cun.es)
We also found that the methylation of PRDM5 promoter was closely related to the histopathological types and the progression of tumors through the public relations database. (jcancer.org)
In this study, we investigated the methylation/hypomethylation status in the promoter region of the mesothelin gene in gynecological tumors. (imrpress.com)
The average methylation of CpG sites in ovarian tumors ranged from 6-56% (median: 31%) in mesothelin-positive and 13-79% (median: 43%) in mesothelin-negative samples. (imrpress.com)
In endometrial tumors, the average methylation ranged from 5-52% (median: 28%) in mesothlin-positive and from 15-67% (median: 22%) in mesothlinnegative samples. (imrpress.com)
We detected diverse levels of methylation/hypomethylation at CpG sites in the mesothelin promoter region in ovarian and endometrial tumors. (imrpress.com)
We uncovered regular promoter methylation of in a big group of tumor cell lines and principal tumors, and confirmed its tumor suppressive demethylation and features activity in tumor cells. (antiviralbiologic.com)
Our findings indicate that aberrant promoter methylation of RASSF1A may contribute to the pathogenesis of many different forms of pediatric tumors. (elsevierpure.com)

Aberrant promoter methylation of insulin-like growth factor binding protein-3 gene in human cancers. (nih.gov)

Recent genome-wide DNA methylation studies have challenged this paradigm, demonstrating that DNA methylation of regulatory regions outside promoters is able to influence cell-type specific gene expression programs under physiologic or pathologic conditions. (cun.es)
Coupling genome-wide DNA methylation assays with histone mark annotation has allowed for the identification of specific epigenomic changes that affect enhancer regulatory regions, revealing an additional layer of complexity to the epigenetic regulation of gene expression. (cun.es)
In this review, we summarize the novel evidence for the molecular and biological regulation of DNA methylation in enhancer regions and the dynamism of these changes contributing to the fine-tuning of gene expression. (cun.es)
Collectively, our data show that DNA methylation is essential and instructive for deposition of specific histone modifications across regulatory regions, which together influences gene expression patterns in mESCs. (ca.gov)
We hypothesized that epigenetic alterations in the promoter region of the dopamine D2 receptor (DRD2) gene would be associated with cue-elicited activation of neural reward regions, as well as severity of alcohol use behavior. (psu.edu)
Whole-blood DNA methylation was characterized at 5.2 million loci by MeDIP sequencing and assessed longitudinally to identify differentially methylated regions associated with high or low pain sensitivity (pain DMRs). (ox.ac.uk)
Non-coding RNAs are linked to mRNA promoter regions, according to research. (wikipedia.org)
Promoters represent critical elements that can work in concert with other regulatory regions (enhancers, silencers, boundary elements/insulators) to direct the level of transcription of a given gene. (wikipedia.org)
I have found some articles which describe methylation in intergenic regions and introns activating transcription but, since you're asking specifically about promoters, I'll limit the examples to methylation in the 5' flanking region. (stackexchange.com)
We hypothesized that diisocyanate (DI) exposure could modify gene promoter regions regulating transcription of cytokine mediators and thereby influence expression of DA. (cdc.gov)

We find that genome-wide DNA demethylation alters occupancy of histone modifications at both promoters and enhancers. (ca.gov)
The reactivation led to transcriptional initiation at the authentic cap sites of the late E2A promoter and was not associated with promoter demethylation, at least not in both DNA complements. (uni-bielefeld.de)
DNA methylation is usually a reversible process, through either passive or active demethylation. (antiviralbiologic.com)
The newly recognized 783348-36-7 IC50 5-hydroxymethylcytosine (5?hmC) in mammalian genomic DNA6, as an intermediate of active DNA demethylation, has been recognized as the sixth base, which provides us new insight into the regulation of CpG methylation dynamics via active demethylation. (antiviralbiologic.com)
Levels of WIF1 promoter methylation, mRNA expression, and protein expression in a glioblastoma cell line were compared before and after demethylation treatment. (gwu.edu)

The CpG hypomethylation in the promoter position -783 of the PPARGC1A gene in leukocytes may represent a biomarker of reduced insulin sensitivity after the ingestion of glucose. (unav.edu)
The methylation-sensitive single-nucleotide primer extension (Ms-SNuPE) technique was used to quantify the methylation/hypomethylation status at 20 CpG sites in the mesothelin promoter region. (imrpress.com)
A correlation was found between mesothelin expression and the average methylation/hypomethylation status as well as methylation/hypomethylation status at four of 20 CpG sites in ovarian samples. (imrpress.com)
We speculate that, although methylation/hypomethylation changes may affect its transcription, other mechanisms may synergically operate in tissue-specific expression and tumor-related mesothelin overexpression. (imrpress.com)
Hypermethylation of CpG islands at active gene promoters leads to transcriptional repression, whereas hypomethylation is associated with gene overexpression. (nih.gov)
Moreover, the effect of Hcy on the expression of TFEB is regulated by hypomethylation of the TFEB promoter catalyzed by DNA methyltransferase 3b (DNMT3b). (lu.se)

Antibodies to CREB1 and NFκB were used to immunoprecipitate formaldehyde-crosslinked protein-DNA fractions, followed by reverse transcription quantitative real-time polymerase chain reaction to quantitate the number of copies of CMV-promoter DNA bound to the various transcription factors. (bournemouth.ac.uk)
METHODS: Methylation specific polymerase chain reaction was used to detect CDKN2A promoter methylation in gastric adenocarcinomas from American patients. (duke.edu)
Promoters can be about 100-1000 base pairs long, the sequence of which is highly dependent on the gene and product of transcription, type or class of RNA polymerase recruited to the site, and species of organism. (wikipedia.org)
RNA polymerase II (RNAP II) bound to the transcription start site promoter can start mRNA synthesis. (wikipedia.org)
Promoters contain specific DNA sequences such as response elements that provide a secure initial binding site for RNA polymerase and for proteins called transcription factors that recruit RNA polymerase. (wikipedia.org)
In bacteria The promoter is recognized by RNA polymerase and an associated sigma factor, which in turn are often brought to the promoter DNA by an activator protein's binding to its own DNA binding site nearby. (wikipedia.org)
In eukaryotes The process is more complicated, and at least seven different factors are necessary for the binding of an RNA polymerase II to the promoter. (wikipedia.org)
A promoter is induced in response to changes in abundance or conformation of regulatory proteins in a cell, which enable activating transcription factors to recruit RNA polymerase. (wikipedia.org)

The MG cells substantially lost their migratory properties after treatment with a methyltransferase inhibitor, 5-azacytidine, suggesting a role of DNA methylation in this process. (oncotarget.com)
Paradoxical role of DNA methylation in activation of FoxA2 gene expression during endoderm development. (stackexchange.com)

Here we examine genome-wide DNA methylation, first in 50 identical twins discordant for heat pain sensitivity and then in 50 further unrelated individuals. (ox.ac.uk)

Patients with hypermethylation of MGMT, p16 INK4a , RASSF1A or RARβ were 5.68 times more likely to have malignant effusions than patients without methylation (p = 0.008). (elsevierpure.com)
Neuroblastoma patients with methylation of RASSF1A were significantly older than patients without methylation (P = 0.008). (elsevierpure.com)

Of women with a BRCA1/2 mutation, none showed methylation of HIN-1 and only 1 of 15 women showed CpG island methylation of RARB M4, INK4a/ARF, or PRB promoters. (umn.edu)

Promoter CpG methylation contributes to ES cell gene regulation in parallel with Oct4/Nanog, PcG complex, and histone H3 K4/K27 trimethylation. (ca.gov)
Teng Jj, Zhao Wj, Zhang Xl, Zhao DK, Qiu XY, Chen Xd, Yang L. Downregulation of promoter methylation gene PRDM5 contributes to the development of tumor proliferation and predicts poor prognosis in gastric cancer. (jcancer.org)
CONCLUSION: The results of this study cannot support the notion that methylation of the pIV promoter of MHC2TA contributes to MS disease risk, although tissue and timing specific epigenetic modifications cannot be ruled out. (ox.ac.uk)

In genetics, a promoter is a sequence of DNA to which proteins bind to initiate transcription of a single RNA transcript from the DNA downstream of the promoter. (wikipedia.org)
CpG methylation can prevent transcription factor binding and/or recruit proteins that inhibit transcription, either competitively or through chromatin condensation. (stackexchange.com)
Epigenetic changes mainly take place through chemical modification of DNA (DNA methylation) and histones, the proteins around which the DNA helix winds itself. (lu.se)
Epigenetic modifications of the histone proteins, such as acetylation or methylation, affect how tightly the DNA is packed around the histones and thus how accessible the DNA sequence is. (lu.se)
As well as DNA methylation, there are also other epigenetic mechanisms such as modification of histone proteins. (lu.se)

We hypothesised that inactivation of BRCA1 by promoter methylation could occur as a germline or an early somatic event that predisposes to breast cancer with the phenotype normally associated with BRCA1 germline mutation. (biomedcentral.com)
In one patient, methylation of 10% of the BRCA1 alleles was detected in the peripheral blood DNA, consistent with 20% of cells having one methylated allele. (biomedcentral.com)
Buccal mucosa DNA from this individual displayed approximately 5% BRCA1 methylation. (biomedcentral.com)
The other patients had no detectable BRCA1 methylation in their peripheral blood. (biomedcentral.com)
These results demonstrate that in some cases of breast cancer, low-level promoter methylation of BRCA1 occurs in normal tissues of the body and is associated with the development of BRCA1 -like breast cancer. (biomedcentral.com)
the earlier in tumorigenesis methylation occurs, the greater the similarity to tumours arising from germline BRCA1 mutations. (biomedcentral.com)
A rare BRCA1 promoter mutation c.-107A>T induces mosaic monoallelic BRCA1 promoter methylation in all tissue types and results in incident breast, ovarian, and colon cancers via the silencing of BRCA1. (jax.org)
The dynamics of this gain of BRCA1 promoter methylation are not well understood. (jax.org)
We showed that the introduction of the c.-107A>T mutation caused initiation and progression of BRCA1 promoter methylation in the WIBJ2 induced pluripotent stem cell line, in cis with the c.-107A>T mutation. (jax.org)
However, we found that, mysteriously, neither of these BRCA1 promoter methylation events occurred in the HEK293T cells. (jax.org)
This work serves as an important step in furthering the scientific community's understanding of developmental gain of methylation at the BRCA1 promoter in the presence of, and irrespective of, the c.-107A>T mutation. (jax.org)
Conclusions: This is the first evidence of CpG island methylation of tumor suppressor gene promoters in non-BRCA1/2 familial breast cancer. (umn.edu)

In contrast, CpG island methylation of RARB M4 (P = 0.051), INK4a/ARF (P = 0.042), HIN-1 (P = 0.044), and PRA (P = 0.032), as well as the overall frequency of methylation events (P = 0.004), was associated with abnormal Masood cytology. (umn.edu)

Evaluation of site-specific methylation of the CMV promoter and its role in CHO cell productivity of a recombinant monoclonal antibody. (bournemouth.ac.uk)
0.0001), no specific methylation event was associated with age. (umn.edu)
We found a complex pattern of CpG specific methylation of this region which was associated with both overall (p=0.044) and disease free (p=0.046) survival. (birmingham.ac.uk)

We hypothesised that one of the mechanisms by which BRAF mutant colorectal cancer demonstrate poor outcomes with chemotherapy is abnormal gene methylation. (birmingham.ac.uk)
We hypothesised that one of the mechanisms by which BRAF mutant colorectal cancer demonstrate poor outcomes with chemotherapy is abnormal gene methylation.METHODS: The Cancer Genome Atlas (TCGA) methylation data was analysed using a linear regression model with BRAF mutation as an independent variable. (birmingham.ac.uk)

The methylation status of IGFBP-3 was determined by bisulfite DNA sequencing and was correlated with expression semi-quantified by real-time RT-PCR to develop a methylation-specific PCR (MSP) assay for IGFBP-3. (nih.gov)
CMV promoter methylation was determined using bisulfite sequencing. (bournemouth.ac.uk)
Bisulfite amplicon sequencing revealed that no significant difference was detected between the methylation levels of CUX1 core promoter region in SM tissues and ST tissues . (bvsalud.org)
Methodology: Using bisulfite sequencing, we investigated the methylation status of five CpG residues of a CpG-rich region in the promoter of SLC6A4 by comparing female shift working nurses from a high work stress environment (n = 24) to low work stress environment (n = 25). (tuni.fi)
Methylation levels were assessed by bisulfite sequencing of DNA extracted from peripheral blood leucocytes. (tuni.fi)
Also, the methylation status of the SOX11 promoter was determined by sodium bisulfite sequencing and methylation specific PCR (MSP). (lu.se)

Distinct promoter methylation patterns arise upon clonal selection in different families of cell lines. (bournemouth.ac.uk)
In this study, we investigated methylation patterns in the promoter region of OAZ1, OAZ2, AMD1, and ARG2 in Brodmann area 44 from a group of 33 suicide completers and 31 non-suicide controls. (ulaval.ca)

The epigenetic regulation through methylation at F7 promoter as well as the inter-relationship of genetic and epigenetic modifications at F7 promoter site were both previously unexplored. (bmj.com)
F7 epigenetic regulation through methylation relates to CAD risk by affecting plasma FVIIa concentrations and its role prevails in those A1A1 subjects, i.e. not carriers of the functional A2 allele. (bmj.com)

Methods: CpG island promoter methylation was tested in the initial random periareolar fine-needle aspiration sample from 109 asymptomatic women at high risk for breast cancer. (umn.edu)
METHODS: In DNA from peripheral blood mononuclear cells from a sample of 50 monozygotic disease discordant MS twins the MHC2TA promoter IV was sequenced and analysed by methylation specific PCR. (ox.ac.uk)
METHODS: The Cancer Genome Atlas (TCGA) methylation data was analysed using a linear regression model with BRAF mutation as an independent variable. (birmingham.ac.uk)

Gene regulation through DNA methylation is a well described phenomenon that has a prominent role in physiological and pathological cell-states. (cun.es)
Reversible Regulation of Promoter and Enhancer Histone Landscape by DNA Methylation in Mouse Embryonic Stem Cells. (ca.gov)
DNA methylation is one of a number of modes of epigenetic gene regulation. (ca.gov)
The correlation between a polymorphism and its effect on DNA methylation and, consequently, on gene product regulation is a novel and very intriguing concept. (bmj.com)
We investigated the relationship between WIF1 promoter methylation and regulation of the WNT/β-catenin signaling pathway, tumor grade, and survival in patients with astrocytoma. (gwu.edu)
The ASXL1 protein may have an additional role in gene regulation by signaling to molecules to add a methyl group (a process called methylation) to an area near a gene called the promoter region, which controls gene activity. (medlineplus.gov)

We previously demonstrated that increased monoclonal antibody productivity in dihydrofolate reductase (DHFR)-amplified CHO cells correlates with phosphorylated transcription factor-cytomegalovirus (CMV) promoter interactions. (bournemouth.ac.uk)
In this article, we extend the characterization to include CMV promoter methylation and its influence on NFκB and CREB1 transcription factor binding to the CMV promoter in two families of DHFR-amplified CHO cell lines. (bournemouth.ac.uk)
Azacytidine treatment reduced methylation in all treated samples, though not at all methylation sites, while increasing transcription. (bournemouth.ac.uk)
Variations in promoters or transcription factors cause some diseases. (wikipedia.org)
These transcription factors have specific activator or repressor sequences of corresponding nucleotides that attach to specific promoters and regulate gene expression. (wikipedia.org)
As promoters are typically immediately adjacent to the gene in question, positions in the promoter are designated relative to the transcriptional start site, where transcription of DNA begins for a particular gene (i.e., positions upstream are negative numbers counting back from -1, for example -100 is a position 100 base pairs upstream). (wikipedia.org)
In bacteria, the promoter contains two short sequence elements approximately 10 (Pribnow Box) and 35 nucleotides upstream from the transcription start site. (wikipedia.org)
There is nothing intrinsic to DNA methylation itself that requires it to repress transcription. (stackexchange.com)
Their hypothesis is that CpG methylation prevents binding of a protein that represses transcription by condensing chromatin through histone modifications. (stackexchange.com)
They hypothesize that methylation could affect chromatin state or recruit, what they term, a methylation dependent factor, which activates transcription. (stackexchange.com)
The results show that the core promoter region of CUX1 was present at (-1601-(-1) bp) upstream of the transcription start site . (bvsalud.org)
Transcription factors bind alongside the promoter and set off the gene expression. (lu.se)

Conclusions: PDCD1 methylation might aid the identification of HNSCC patients potentially benefitting from a radical or alternative treatment, particularly in the context of immunotherapies targeting PD-1/PD-L1. (uni-koeln.de)
CONCLUSIONS: The presence of EBV in gastric adenocarcinomas is strongly associated with CDKN2A inactivation by promoter methylation. (duke.edu)
Conclusions: Our findings show that environmental stress is concurrent with decreased methylation of the SLC6A4 promoter. (tuni.fi)

It will now be demonstrated that the inactivation of the late promoter of Ad2 DNA can be reversed by transactivating functions that are encoded in the 13S messenger RNA of the E1A region of Ad2 DNA. (uni-bielefeld.de)

The current study leveraged functional neuroimaging (fMRI) during an alcohol reward paradigm (n = 383) to test associations among DRD2 promoter methylation in peripheral tissue, signal change in the striatum during the presentation of alcohol cues, and severity of alcohol use disorder (AUD). (psu.edu)

Methylation in CpG sites of the PPARGC1A gene (encoding PGC1-α) has been associated with adiposity, insulin secretion/sensitivity indexes and type 2 diabetes. (unav.edu)
We assessed the association between the methylation profile of the PPARGC1A gene promoter gene in leukocytes with insulin secretion/sensitivity indexes in normoglycemic women. (unav.edu)
An in vivo study confirmed that methylation adjuvant therapy can improve the sensitivity of cancer to EGFR-targeted therapy. (geneticsmr.com)
promoter was significantly increased in DA+ in comparison with both comparator groups (DA- and AW), and it exhibited good sensitivity (77.5%) and specificity (80%) for identifying DA workers in a multivariate predictive model after adjusting for type of DI exposure, smoking status, methacholine PC20, and gender. (cdc.gov)

We also studied promoter region methylation of PRDM5 in GC by methylation-specific PCR (MSP). (jcancer.org)
In the other cell line family, lower methylation frequency at the NFκB consensus binding site was accompanied by more NFκB recruitment to the promoter region. (bournemouth.ac.uk)
A strong correlation between the DNA methylation percentage of different CpG sites of the PPARGC1A promoter in leukocytes was found, suggesting an integrated epigenetic control of this region. (unav.edu)
Reference: [DNA methylation of human telomerase reverse transcriptase promoter region in human ameloblastoma]. (neb.com)
It was demonstrated in transient expression experiments that the 5' CCGG 3'-methylated late E2A promoter was at least partly reactivated in cell lines constitutively expressing the E1 region of Ad2 or of adenovirus type 5 (Ad5) DNA. (uni-bielefeld.de)
As DNA methylation can regulate the gene expression , the potential association between CUX1 core promotor region methylation and lambskin pattern in Hu sheep was explored in the present study. (bvsalud.org)
A repressive region (-1151-(-751) bp) was also detected, which had a strong inhibitory effect on CUX1 promoter activity. (bvsalud.org)
Although the data demonstrated the differential expression of CUX1 between SM and ST probably has no association with DNA methylation , the identification of the core region and a potential repressive region of CUX1 promoter can enrich the role of CUX1 in Hu sheep hair follicle development. (bvsalud.org)
NSCLC cells with low expression of EGFR and high methylation in the promoter region were insensitive to EGFR-targeted therapy. (geneticsmr.com)
This probe covered a region -413bp from the promoter region of TFAP2E. (birmingham.ac.uk)
When a promoter region is methylated, gene activity is repressed, and when a promoter region is not methylated, the gene is active. (medlineplus.gov)

This study reports that, during development, high levels of methylation of a CpG island in the FoxA2 promoter is present in expressing tissues and absent in non-expressing tissues. (stackexchange.com)
The authors analyzed 16 matched normal and tumor tissues for aberrant DNA methylation using the methylated CpG island amplification and microarray method and the pyrosequencing technique. (nih.gov)
RASSF1A methylation was tumor specific and was absent in adjacent non-malignant tissues. (elsevierpure.com)

Here we use nucleosome occupancy and methylation sequencing method to analyze both the genome-wide chromatin accessibility and DNA methylome at a series of crucial time points during fetal germ cell development in both human and mouse. (nature.com)

We treated six cell lines lacking RASSF1A mRNA with 5-aza-2′deoxycytidine to examine the relationship between methylation and transcriptional silencing. (elsevierpure.com)
Further analyses of EOC cell lines showed that SOX11 mRNA and protein were expressed in two of five cell lines, correlating with promoter methylation status. (lu.se)

Promoter methylation of WNT inhibitory factor-1 and expression pattern" by Sun A. Kim, Jihye Kwak et al. (gwu.edu)

In summary, our results showed that IGFBP-3 methylation played an important role in the silencing of its expression, suggesting that IGFBP-3 may act as a tumor suppressor gene in several human cancers examined. (nih.gov)
In gastric cancers without EBV, 32% exhibited CDKN2A promoter methylation and 26% had p16 protein loss. (duke.edu)

Several pain DMRs show longitudinal stability consistent with susceptibility effects, have similar methylation levels in the brain and altered expression in the skin. (ox.ac.uk)
Methylation of class II transactivator gene promoter IV is not associated with susceptibility to multiple sclerosis. (ox.ac.uk)

Allelic methylation is functionally equivalent to a mutation in that loss of activity of the second allele arising from a mutation, loss of heterozygosity or a second methylation event will inactivate the gene. (biomedcentral.com)
To deconvolute this process, we introduced this c.- 107A>T mutation in two in vitro cell lines and analyzed them for their methylation status at 30, 60, and 100 days in culture. (jax.org)

DNA methylation also reversibly regulates H3K27ac and H3K27me3 at previously identified tissue-specific enhancers. (ca.gov)
We hypothesized that maternal gestational glucose levels would positively correlate with DNA methylation of the PPARGC1A promoter in placental tissue. (portlandpress.com)
The mean percent methylation of the WIF1 promoter in astrocytomas was higher than that in control brain tissue. (gwu.edu)

Here, we profile the DNA methylome, transcriptome, and global occupancy of histone modifications (H3K4me1, H3K4me3, H3K27me3, and H3K27ac) in a series of mouse embryonic stem cells (mESCs) with varying DNA methylation levels to study the effects of DNA methylation on deposition of histone modifications. (ca.gov)

Other mechanisms such as DNA methylation regulate gene expression via epigenetic phenomena. (bmj.com)

Similarly, the majority of CRC cell lines tested exhibited decreased BVES expression and promoter DNA hypermethylation, a modification associated with transcriptional silencing. (jci.org)
Treatment with a DNA-demethylating agent restored BVES expression in CRC cell lines, indicating that methylation represses BVES expression. (jci.org)
Our data suggest that in mES cells promoter methylation represents a unique epigenetic program that complements other regulatory mechanisms to ensure appropriate gene expression. (ca.gov)
Promoters control gene expression in bacteria and eukaryotes. (wikipedia.org)
Gene expression depends on promoter binding. (wikipedia.org)
The modification of the three 5' CCGG 3' sequences at nucleotides +24, +6 and -215, relative to the cap site in this promoter, sufficed to silence the gene in transient expression either in Xenopus laevis oocytes or in mammalian cells, and after the fixation of the E2A promoter-chloramphenicol-acetyltransferase (CAT) gene construct in the genome of hamster cells. (uni-bielefeld.de)
Can methylation of a promoter induce gene expression in some rare cases? (stackexchange.com)
I've read somewhere that methylation of an intron can induce gene expression (eg. (stackexchange.com)
begingroup$ @Chris: I'm intersted if a methylation of a promoter can induce gene expression in some rare cases, but I've only read about introns- if they are metilated they can induce gene expression. (stackexchange.com)
Here, PDPN expression was associated with CpG methylation. (stackexchange.com)
We found significant site-specific differences in methylation in the promoter of ARG2 and AMD1 that were also significantly negatively correlated with gene expression. (ulaval.ca)
EGFR expression was high when the methylation degree was lower in patients with adenocarcinoma and poor pathological differentiation of tumor than in patients with squamous cell carcinoma and good pathological differentiation. (geneticsmr.com)
In summary, this study shows that Hcy can activate autophagy by inhibiting DNMT3b-mediated DNA methylation and upregulating TFEB expression. (lu.se)

Misunderstandings can result from using canonical sequence to describe a promoter. (wikipedia.org)
On average, only 3 to 4 of the 6 base pairs in each consensus sequence are found in any given promoter. (wikipedia.org)
The inactivating effect of sequence-specific promoter methylations was extensively studied by using the late E2A promoter of adenovirus type 2 (Ad2) DNA. (uni-bielefeld.de)
Activation of a methylated promoter mediated by a sequence-specific DNA-binding protein, RFX. (stackexchange.com)
RESULTS: No methylation or sequence variation of the MHC2TA promoter pIV was found. (ox.ac.uk)
In front of every gene is a DNA sequence called a promoter. (lu.se)

Methylation levels were tested by several quantitative techniques including MethyLight, methylation-sensitive high resolution melting (MS-HRM) and a newly developed digital MS-HRM assay. (biomedcentral.com)
In unadjusted (bivariate) analysis, burnout was not significantly associated to methylation levels. (tuni.fi)
0.0001 respectively) to methylation levels. (tuni.fi)

Results: High PDCD1 methylation (mPDCD1) was associated with a significantly shorter overall survival after surgical resection in both the discovery (HR = 2.24 [95% CI: 1.08-4.64], p = 0.029) and the validation cohort (HR = 1.54 [95% CI: 1.08-2.21], p = 0.017). (uni-koeln.de)

0.0001), suggesting the presence of ethnic differences in the IGFBP-3 methylation status. (nih.gov)
In contrast, 91% of the tumours containing EBV had CDKN2A promoter methylation (p = 0.0003) and 90% showed p16 protein loss (p = 0.0001). (duke.edu)

Regulatory elements can be several kilobases away from the transcriptional start site in gene promoters (enhancers). (wikipedia.org)
The distal promoter is upstream of the gene and may contain additional regulatory elements with a weaker influence. (wikipedia.org)
The reactivation of a methylation-inactivated eukaryotic promoter by transactivating functions has general significance in that the value of a regulatory signal can be fully realized only by its controlled reversibility. (uni-bielefeld.de)
Promoter CpG methylation is a fundamental regulatory procedure for gene appearance. (antiviralbiologic.com)

We also found that IGFBP-3 methylation was preferentially present in GCs arising in the lower-third of the stomach (p = 0.079). (nih.gov)

In multivariate Cox proportional hazards analysis, PDCD1 methylation remained a significant prognostic factor for HNSCC (HR = 2.14 [95% CI: 1.19-3.84], p = 0.011). (uni-koeln.de)

Anatomy22

Organisms6

Diseases8

Chemicals and Drugs104

Analytical, Diagnostic and Therapeutic Techniques and Equipment26

Phenomena and Processes99

Disciplines and Occupations2

Information Science3

Health Care2

Genes32

Examined the methylation status1

Proximal promoter6

Association between promoter methylation1

Tumors8

Aberrant promoter methylation1

Regions10

Demethylation5

Hypomethylation6

Polymerase8

Role of DNA methylation2

Genome-wide DNA methylation1

Patients without methylation2

Showed CpG island1

Contributes3

Proteins5

BRCA112

Island methylation1

Specific methylation3

Gene methylation2

Bisulfite6

Patterns2

Epigenetic regulation2

METHODS3

Regulation6

Transcription12

Conclusions3

Inactivation1

Peripheral1

Sensitivity4

Region11

Tissues3

Chromatin1

MRNA2

Inhibitory1

Cancers2

Susceptibility2

Mutation2

Tissue3

Histone modifications1

Regulate1

Expression13

Sequence6

Levels3

Significantly1

0.00012

Regulatory4

Preferentially1

Prognostic factor1