• This project has generated high-quality genome-wide maps of several key histone modifications, chromatin accessibility, DNA methylation and mRNA expression across 100s of human stem cells and primary ex vivo tissues selected to represent the normal counterparts of tissues and organ systems frequently involved in human disease. (expasy.org)
  • The methylation occurs at the N6 of adenines in (5′)GATC sequences. (slideserve.com)
  • Transcriptome sequencing is important to researchers because they help us understand how the cellular machinery interprets genomic sequences and alterations to these sequences. (cd-genomics.com)
  • We focus on three contexts: (1) prenatal genomic sequencing for possible fetal abnormalities, (2) rapid genomic sequencing for critically ill children, and (3) reanalysis of genomic data obtained from children for diagnostic purposes. (cdc.gov)
  • The implications of these findings with regard to the maintenance of methylation-free islands, X chromosome inactivation, and the chromatin structure of facultative heterochromatin are discussed. (oregonstate.edu)
  • Previous research has shown that DNA methylation can drive speciation and modulate virulence in bacteria, but the impact of epigenetic modifications on the diversification and pathogenesis of R. solanacearum is unknown. (pacb.com)
  • Epigenetic control of gene expression lasts through multiple cell divisions without alterations in primary DNA sequence and can occur via mechanisms that include histone modification and DNA methylation. (stanford.edu)
  • Our analysis identified a novel methylation motif associated with a DNA methylase that is conserved in all complete Ralstonia spp. (pacb.com)
  • We have developed MotifLab as a flexible workbench for motif analysis in a genomic context. (biomedcentral.com)
  • N 6 -deoxyadenosine methylation (6mA) is the most widespread type of DNA modification in prokaryotes and is also abundantly distributed in some unicellular eukaryotes. (nature.com)
  • Using this method, we examined the genomic positions of 6mA in bacteria ( E. coli ), green algae ( C. reinhardtii ), and mammalian cells (HEK239T, Huh7, and HeLa cells). (nature.com)
  • MISMATCH REPAIR: only viable a short time after replication Methylation and mismatch repair Really only understood well in E.coli. (slideserve.com)
  • In vivo footprint and methylation analysis by PCR-aided genomic sequencing: comparison of active and inactive X chromosomal DNA at the CpG island and promoter of human PGK-1. (oregonstate.edu)
  • We used ligation-mediated polymerase chain reaction (PCR) for a genomic sequencing study in which 450 bp of the human PGK-1 promoter region was analyzed for the presence of in vivo protein footprints and cytosine methylation at all CpG sites. (oregonstate.edu)
  • Analysis of DNA methylation status of the promoter of human telomerase reverse transcriptase in gastric carcinogenesis. (neb.com)
  • Embryonic stem cells (ESCs), derived from the blastocyst-stage embryo, are capable of generating all cell types of the mammalian body (pluripotency) and of maintaining the capacity for indefinite self-renewal without compromising their genomic integrity. (biomedcentral.com)
  • Connect-four: genomic analyses of regenerating stem cells identifies zygotic Dux factors as tumor initiators. (mpi-hlr.de)
  • From the abstract: ' This paper examines the ethics of introducing emerging forms of artificial intelligence (AI) into prenatal and pediatric genomic medicine. (cdc.gov)
  • The two studies demonstrate the importance of population-level data to track progress in implementation, outcomes, and disparities in genomic medicine. (cdc.gov)
  • Due to the prominence of high-throughput sequencing technology, genomic data such as RNA-seq has become commonly accessible. (cd-genomics.com)
  • In vivo footprinting studies with dimethylsulfate (DMS) revealed eight regions of apparent protein-DNA contacts on the Xa. (oregonstate.edu)
  • The SNPs identified by GWAS that are statistically significantly over-represented in the disease (or case) populations are called risk-associated SNPs and genomic regions containing the SNPs are called risk loci for that particular disease. (biomedcentral.com)
  • In htz1Delta cells, Sir2 and Sir3 spread into flanking euchromatic regions, producing changes in histone H4 acetylation and H3 4-methylation indicative of ectopic heterochromatin formation. (sdbonline.org)
  • For a reverse experiment, DNA methylation in early Xenopus embryos was assessed by MBD affinity capture. (biomedcentral.com)
  • Application of genomic AI to these early life settings has not received much attention in the ethics literature. (cdc.gov)
  • 4-7 Repeat expansion into the full mutation size range usually coincides with de novo methylation of the fragile X chromosome region. (bmj.com)
  • 3 8 De novo methylation is thought to be initiated at embryogenesis by attraction of DNA methyl transferase to hairpin-like unimolecular fold backs of CGG repeat sequences expanded beyond a threshold of repeat length. (bmj.com)
  • We also study the patterning of de novo DNA methylation in fetal male germ cells. (vai.org)
  • The Zebrafish Information Network (ZFIN, http://zfin.org), the model organism database for zebrafish, provides the central location for curated zebrafish genetic, genomic and developmental data. (shengsci.com)
  • To quantify cis- versus trans-acting contributions to mammalian regulatory evolution, we performed genomic DNase I footprinting of the mouse genome across 25 cell and tissue types, collectively defining ~8.6 million transcription factor (TF) occupancy sites at nucleotide resolution. (wisc.edu)
  • We construct a high-resolution pan-genome dataset representing almost the entire genomic content in the silkworm. (cnrs.fr)
  • Both the soma-germline and the germline-soma transitions involve global erasure and reestablishment of DNA methylation patterns. (vai.org)
  • To elucidate whether the Kcnq1ot1 RNA plays a role in the bidirectional silencing activity of the Kcnq1 ICR, we have characterized factor binding sites by genomic footprinting and tested the functional consequence of various deletions of these binding sites in an episome-based system. (nih.gov)
  • Taken together, our population-scale genomic resources will promote functional genomics studies and breeding improvement for silkworm. (cnrs.fr)
  • From the abstract: ' Increasing demand for genomic testing coupled with genetics workforce shortages has placed unsustainable pressure on standard models of care. (cdc.gov)
  • From the abstract: ' Automating reanalysis of genomic data for undiagnosed rare disease patients presents a paradigm shift in how clinical genomics is delivered. (cdc.gov)
  • Changes in methylation pattern of albumin and alpha-fetoprotein genes in developing rat liver and neoplasia. (shengsci.com)
  • We find that the silkworm population harbors a high density of genomic variants and identify 7308 new genes, 4260 (22%) core genes, and 3,432,266 non-redundant structure variations (SVs). (cnrs.fr)
  • Despite increased interest in archaeal chromatin in recent years, its properties have been little studied using genomic tools. (biomedcentral.com)
  • Other genomic elements code for the expression and processing of RNA transcripts. (mdpi.com)