• Chromogenic in situ hybridization (CISH) is a recently introduced method, and although it makes use of the in situ hybridization technology of FISH, it also takes advantage of the chromogenic signal detection of IHC that can be detected with the ordinary light microscope and costs one-quarter as much as FISH. (biomedcentral.com)
  • Detection of cytomegalovirus by in situ hybridisation and immunohistochemistry using new monoclonal antibody CCH2: a comparison of methods. (bmj.com)
  • Moreover, zebrafish is very well-suited for systematic analysis of gene expression patterns at transcript level by whole-mount in situ hybridization (WISH), and at protein level by immunohistochemistry/immunofluorescence (IHC/IF). (frontiersin.org)
  • Tumor Her2/ neu is generally assessed as protein overexpression by using immunohistochemistry (IHC), and patients with tumors that either have 2+ or 3+ results with this method become good candidates for treatment with trastuzumab. (biomedcentral.com)
  • Three-color in situ hybridization was applied to the study of numerical chromosome abnormalities as occur in human solid tumors. (nih.gov)
  • To study the incidence of trisomy 3 and its implications for the pathogenesis of PGL in Hong Kong, we have applied the technique of chromosome in situ hybridization in 13 cases of PGL by using archival paraffin-embedded tissue sections. (hku.hk)
  • METHODS: Translocations were scored using fluorescence in situ hybridization chromosome painting. (cdc.gov)
  • High-resolution chromosome analysis uses special culture methods to obtain a high percentage of prophase and prometaphase spreads. (msdmanuals.com)
  • This method is compatible with the standard workflow of conventional FISH, allows re-budgeting of the sample for various tests, and results in a ~ 15-fold reduction in probe consumption. (springer.com)
  • The continuous flow of probes and shaping liquid on these selected cells resulted in a 120-fold reduction of the hybridization time compared with the standard protocol (3 min vs. 6 h) and efficient rinsing, thereby shortening the total FISH assay time for centromeric probes. (springer.com)
  • A key milestone in ISH-based techniques was fluorescence in situ hybridization (FISH) (Bauman et al. (springer.com)
  • Advances in fluorescence microscopy, fluorescent dyes, biotechnology, bioinformatics, and research on the human genome project in the late 90's accelerated the development of methods to synthesize and design NA probes for FISH. (springer.com)
  • In this paper, we present a new method to implement micrometer-scale interphase FISH that we term μFISH. (springer.com)
  • Therefore, we analyzed the potential of fluorescence in situ hybridization (FISH) for diagnosing WD, using a Tropheryma (T.) whipplei -specific probe. (frontiersin.org)
  • Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that uses fluorescent probes that bind to only particular parts of a nucleic acid sequence with a high degree of sequence complementarity. (wikipedia.org)
  • To develop and evaluate an automated method for quantification of HER2 fluorescence in situ hybridisation (FISH) signals. (bmj.com)
  • The FISH quantification tool (FishJ) is available for download as an ImageJ macro or alternatively it can be utilised through a web interface with an option of uploading FISH images for hybridisation signal counting. (bmj.com)
  • The authors studied DNA amplification of the HER-2/neu gene on 4-microm sections obtained from 62 formalin-fixed, paraffin-embedded PCs by fluorescence in situ hybridization (FISH). (nih.gov)
  • Also, FISH is more sensitive than IHC for detection of abnormalities in the HER-2/neu gene, and further studies should be undertaken to determine whether a FISH-based HER-2/neu detection method may prove of importance in the prediction of prognosis and planning of therapy in prostate cancer patients. (nih.gov)
  • Her-2/ neu gene amplification is primarily detected by in situ hybridization and uses fluorescence (FISH) to detect the signals. (biomedcentral.com)
  • In this analysis, HER2 results obtained with Onco type DX, a validated 21-gene recurrence score assay utilizing RT-PCR, were compared with standard fluorescence in situ hybridization (FISH) results. (biomedcentral.com)
  • Furthermore, FISH is a fast and economical method to detect and identify the zoonotic agent Campylobacter within ovine abortion material. (dtu.dk)
  • It is the recommended first-tier test for patients undergoing prenatal diagnosis for the indication of a fetal structural abnormality detected by ultrasound (unless the structural abnormality is strongly suggestive of a specific aneuploidy, in which case, karyotype with or without fluorescence in situ hybridization [FISH] may be offered before genomic microarray). (arupconsult.com)
  • Fluorescence in-situ hybridization (FISH) was subsequently tested in conjunction with a range of oligonucleotide probes for the detection of legionella in environmental samples. (dguv.de)
  • Methods Adult AML samples at diagnosis or follow-up with karyotype and FISH were identified. (medscape.com)
  • It is common practice for laboratories to use interphase fluorescence in situ hybridization (FISH) panels in conjunction with karyotyping for the detection of the most prognostically significant recurrent AML-associated cytogenetic abnormalities, both in diagnostic specimens and during follow-up to monitor response to therapy. (medscape.com)
  • Fluorescence in situ hybridization (FISH) is also an acceptable method, with ROS1positivity defined as the detection of at least 15% of neoplastic nuclei with ROS1 gene rearrangements among a minimum of 50 total neoplastic nuclei. (who.int)
  • Fluorescence "in situ" hybridization (FISH) technique enables chromosomes to be tagged with fluorescent dyes to highlight genetic traits. (lu.se)
  • Examples of acceptable methods include next-generation sequencing (NGS), Sanger sequencing, reverse transcriptase-polymerase chain reaction (RT-PCR), NanoString and EdgeSeq. (who.int)
  • It relies on the sequence-specific hybridization of probes to their complementary targets in individual cells, followed by direct or indirect detection of the labelled probe. (springer.com)
  • For this purpose, a number of fluorescence-based methods, such as the use of genetic probes and a range of dyes, were to be tested on laboratory and industrial samples. (dguv.de)
  • The treatment of non-muscle-invasive bladder cancer (Ta, T1, carcinoma in situ [CIS]) begins with transurethral resection of bladder tumor (TURBT). (medscape.com)
  • Carcinoma in situ (CIS) is frequently found in association with high-grade or extensive TCC. (medscape.com)
  • The classic appearance of carcinoma in situ as a flat, velvety patch. (medscape.com)
  • However, using special staining techniques such as 5-aminolevulinic acid, it has been shown that significant areas of carcinoma in situ are easily overlooked by conventional cystoscopy. (medscape.com)
  • Detects small copy number variants (CNVs) and further characterizes chromosomal abnormalities identified by conventional cytogenetic methods on direct and cultured amniotic fluid and chorionic villus sampling (CVS) specimens. (arupconsult.com)
  • Comparative Genomic Hybridization, or CGH, is a useful cytogenetic diagnostic tool when a diagnosis is unknown. (psychesystems.com)
  • The goal of this work was to use cytogenetic and molecular methods to determine the species delimitations and understand the karyoevolution of S . marmoratus using samples collected from distinct Brazilian localities. (scielo.br)
  • This programme is operated by UK NEQAS Immunocytochemistry & In-Situ Hybridisation . (ukneqas.org.uk)
  • For further information on the operation of the programme or details on current accreditation status please contact UK NEQAS Immunocytochemistry & In-Situ Hybridisation directly. (ukneqas.org.uk)
  • Details of the analysis procedures applied to data can be obtained from UK NEQAS Immunocytochemistry & In-Situ Hybridisation directly. (ukneqas.org.uk)
  • Details of the performance criteria for this programme can be obtained from UK NEQAS Immunocytochemistry & In-Situ Hybridisation directly. (ukneqas.org.uk)
  • In 1988 the Service was recognised by the UK Department of Health and from that time it became known as the UK National External Quality Assessment Service for Immunocytochemistry (UK NEQAS-ICC and later, when it incorporated in-situ hybridisation methods, as UKNEQAS ICC & ISH). (ukneqas.org.uk)
  • Further applications of this method in prenatal diagnosis for the detection of aneuploidy of the most frequently involved autosomes, as well as for the quantification of gene copy number and mRNA expression, are discussed. (nih.gov)
  • CME: What is the Preferred Molecular Testing Method to Detect RET in NSCLC? (reachmd.com)
  • Today I'll be discussing what is the preferred molecular testing method to detect RET fusions in non-small cell lung cancer. (reachmd.com)
  • The drawback of this method is that it cannot detect balanced inversions or translocations. (psychesystems.com)
  • This study in Iraq used in situ hybridization to detect the frequency and genotyping of HPV in tissue specimens from 129 patients diagnosed with malignant breast cancer, 24 with benign breast tumours and 20 healthy controls. (who.int)
  • Guidelines mandate demonstration of 95% concordance to another laboratory or method before reporting patient HER2 results. (biomedcentral.com)
  • Fifty-five patients were HER2 + by both methods. (biomedcentral.com)
  • Using diagnostic tumour tissue specimens from 95 patients allowed capturing the expression in situ. (helsinki.fi)
  • METHODS Synovial tissue specimens from 40 patients with RA and eight patients with non-rheumatic disease were obtained by arthroscopy guided biopsy techniques or during joint surgery. (bmj.com)
  • Based upon the studies conducted, the hybridization technique was developed further, the time required for processing of the samples reduced, and the quality of the fluorescence microscopy specimens improved. (dguv.de)
  • The objective of the project was to develop a method employing fluorescence microscopy for the detection of legionella in industrial samples from a range of working areas. (dguv.de)
  • carry out studies where biological structures or functions are visualised using methods based on fluorescence microscopy. (lu.se)
  • understand the limitations and possibilities when using different methods based on light and fluorescence microscopy. (lu.se)
  • The process is done in 3 main procedures: tissue preparation (pre-hybridization), hybridization, and washing (post-hybridization). (wikipedia.org)
  • After fixation, samples are permeabilized to allow the penetration of hybridization reagents. (wikipedia.org)
  • Il est argumenté que cette localisation est en accord avec l'hypothèse d'osmorégulation de la fixation symbiotique d'azote. (agronomy-journal.org)
  • The hybridization signals for each probe when a nucleic abnormality is detected. (wikipedia.org)
  • After checking all the necessary conditions, hybridization steps can be started by first adding a target-specific probe, composed of 20 oligonucleotide pairs, hybridizes to the target RNA(s). (wikipedia.org)
  • In situ hybridization of a radioactive RNA probe on resin-embedded legume root-nodule sections: a tool for observing gene expression in the rhizosphere? (agronomy-journal.org)
  • In this work we performed in situ hybridization of a carbonic anhydrase RNA 35 S-labeled probe on nodule sections previously embedded in a methacrylate resin. (agronomy-journal.org)
  • The aim of the method is to isolate fixed chromatin from Drosophila embryos. (embl.de)
  • Western blot analysis and in situ hybridization confirmed the presence of AND-34 RNA and protein in lens epithelial cells, particularly at the lens equator. (molvis.org)
  • Computerised image analysis methods provided comparative quantitative assessments. (bmj.com)
  • METHODS: This was a descriptive analysis of Wisconsin COVID workers' compensation (WC) claims between March 12 and December 31, 2020. (cdc.gov)
  • A method is described for visualizing three nucleic acid sequences simultaneously by in situ hybridization using a new blue immunofluorescent label, amino methyl coumarin acetic acid (AMCA), in combination with green and red fluorescing FITC and TRITC. (nih.gov)
  • Hybridisation signals were calculated both manually and automatically with the ImageJ custom macro. (bmj.com)
  • De plus, la qualité de ces résultats avec une sonde radioactive suggère qu'il serait possible d'utiliser l'hybridation in situ pour des applications telles que la localisation et la quantification d'une invasion microbienne de l'endorhizosphère ou du rhizoplan en utilisant des sondes ARN spécifiques de champignons ou de bactéries, et en comptant le nombre de signaux radioactifs par cellule. (agronomy-journal.org)
  • This was to be followed by standardization, quantification and validation of the methods. (dguv.de)
  • It was not possible at this stage to formulate a standard method for the detection of legionella from industrial samples which described direct detection of the legionella on a filter and computer-aided quantification of the cells. (dguv.de)
  • As a detection method, it does not represent an improvement over the established method (cultivation on selective media and detection by means of a latex agglutination test), since the combination of commercial test system and fluorescence microscope entails relatively complex laboratory apparatus and expensive test kits. (dguv.de)
  • The reasons for this are, on the one hand, the sensitivity of the method to interference from chemical substances in these samples, and on the other, the associated microbiological flora, which is generally present here in high concentrations and which presents an obstacle to the automated microscopic detection of legionella. (dguv.de)
  • METHODS: During September 2020 - May 2021, the Wisconsin Department of Health Services collected occupation and industry data as part of routine COVID-19 case investigations. (cdc.gov)
  • Like the conventional DIN detection method, the rapid test which was examined requires that single colonies be cultivated beforehand. (dguv.de)
  • In situ hybridization (ISH) also was performed in this study, and the primer pair MM-like (CCTGTCCTATGTCGGGCAGG) hybridized with the Pacific oyster parasite. (alr-journal.org)
  • It is critical for the hybridization process to have all optimal conditions to have a successful in situ result, including temperature, pH, salt concentration, and time of the hybridization reaction. (wikipedia.org)
  • What is in situ hybridization and what are the recent advancements with this indispensable technique? (enzolifesciences.com)
  • The hybridization technique developed in the "Development of a quick test for determination of legionella pneumophila by molecular biological methods" project No. FFFF0202 was to be optimized, and the time required for processing of the samples reduced as a result. (dguv.de)
  • L'étude menée en Iraq a utilisé la méthode d'hybridation in situ pour déterminer la fréquence du papillomavirus humain et pour son génotypage dans les échantillons de tissus prélevés auprès de 129 patientes ayant reçu un diagnostic de cancer du sein malin, de 24 patientes porteuses d'une tumeur du sein bénigne et de 20 femmes témoins en bonne santé. (who.int)
  • In situ conservation is a wonderful method to protect an endangered plan or animal species in its natural area, either by safeguarding the habitat itself, or by protecting the species from the predators. (differencebetween.net)
  • This method ensures not only multiplication of the species, but process of evolution and adaptation as well. (differencebetween.net)
  • Conservation of species in in situ takes place in their natural habitats. (differencebetween.net)
  • Ex situ conservation involves plant and animal species. (differencebetween.net)
  • Denaturing allows hybridization to occur after the two samples mix with each other. (psychesystems.com)
  • Theoretical introduction to digital visualisation with an emphasis on fluorescence-based methods and digital image processing. (lu.se)
  • Further work is required in this area in order for the hybridization and staining protocol to be optimized for different samples. (dguv.de)
  • A modified immunohistochemical method detecting cytokine producing rather than cytokine binding cells was applied to determine cytokine synthesis in fixed cryopreserved sections. (bmj.com)
  • This approach involves the intramolecular cross-linking of 5′ amine- and 3′ disulfide-modified MO oligonucleotides using appropriately functionalized tethers, generating macrocyclic structures that conformationally resist RNA hybridization. (cdc.gov)
  • In a protocol every step of a method is precisely defined. (lu.se)
  • It is a cheap and convenient method of conserving biological diversity. (differencebetween.net)
  • In this method of biological diversity conservation, sampling, shifting, storage and preservation of target taxa is carried out outside the natural habitat of the organisms. (differencebetween.net)
  • It is conservation of biological diversity outside their natural areas and is done through different methods like captive breeding, botanical garden, zoos, aquaria etc. (differencebetween.net)
  • Development of methods for si/shRNA knockdown in airway cell models. (unc.edu)