Methods quantification. Medical search. Frequent questions

However, they are present at nanomolar concentrations in biological samples, posing challenges for detection and quantification. (biorxiv.org)
Detection of analytes was performed using an ultra-high performance liquid chromatography method coupled to tandem mass spectrometry (UHPLC-MS/MS) in dynamic multiple reaction monitoring (dMRM) mode. (biorxiv.org)
Although culture is the standard method for definitive diagnosis, detection of cryptococcal antigen (CRAG) in serum or cerebrospinal fluid (CSF) is used for presumptive diagnosis. (cdc.gov)
Based on the levels of SARS-CoV-2 in wastewater, methods can be adapted to higher or lower detection limits as needed. (cdc.gov)
Cassiday L. Two new methods for ricin detection. (cdc.gov)
Puri P and Kumar O. Integrating immunobased detection and identification methods for ricin analysis: an overview. (cdc.gov)
The method was compared with an immunoturbidimetric procedure for the detection of microalbumuniuria. (who.int)
The reliability and practicability of the TCA method renders it suitable for the detection of early stage renal damage, with emphasis on screening high- risk populations in countries with limited resources. (who.int)
The detection limit (DL, LOD, LLD) for a chemical analysis method is the lowest concentration at which a substance can be detected. (janusinfo.se)
Due to the absence of chemical reference materials for lignin dimers and beyond, universal detection methods based on i.e. response factor prediction are being developed. (lu.se)

This assay is based on a microbiological method conducted in petri dishes or multi-well cell culture plates. (wikipedia.org)
Consideration of the immunoassay method and reliable assay-specific reference ranges are of particular importance especially in clinical cases where small changes in insulin levels can cause false classification in terms of insulin sensitivity of horses and ponies. (biomedcentral.com)
After preparing the fluorescence assay, DNA quantification is carried out using a fluorometer, which compares the fluorescence of a given sample to a standard curve and a set of DNA samples of known concentration. (denovix.com)

sleuth is implemented in an interactive shiny app that utilizes kallisto quantifications and bootstraps for fast and accurate analysis of data from RNA-seq experiments. (nature.com)
At the heart of this lies a common need for rapid and accurate methods to measure algal biomass concentrations. (frontiersin.org)
These results demonstrate that the developed method can be a fast and accurate method for quantification of silibinin in aqueous samples. (researchsquare.com)
Quantification of coronary plaque within selected proximal or middle coronary segments without image blurring and heavy calcification with 64-slice CT was moderately accurate with respect to intravascular ultrasound and demonstrated good reproducibility. (escardio.org)
DNA quantification is a fundamental laboratory process that enables the accurate analysis of genetic samples. (denovix.com)
This method is generally more time-consuming than spectrophotometry or fluorescence quantification and less accurate in terms of quantification but will provide information on size distribution of DNA fragments. (denovix.com)
Title : Accurate quantification of tio2 nanoparticles collected on air filters using a microwave-assisted acid digestion method Personal Author(s) : Mudunkotuwa, Imali A.;Anthony, T. Renée;Grassian, Vicki H.;Peters, Thomas M. (cdc.gov)
However, accurate quantification can be challenging, and many researchers find it difficult to study in a robust manner. (lu.se)
By using dose-response curve principles for both experimental design and analysis, it is possible to increase the sensitivity and robustness, leading to accurate quantification of phagocytosis that is comparable across experiments and systems.Here, we describe how to quantify phagocytosis using flow cytometry with a robust, high-throughput, and easy-to-use approach. (lu.se)

region 228440 to 229981) was the amplification target for quantification of hr DNA by PCR. (scirp.org)
However, all of these methods involve PCR amplification of target DNA that is typically more than hundred base-pair long [ 25 - 30 ]. (hindawi.com)
Real-time PCR or quantitative PCR (qPCR) techniques combine DNA amplification with DNA quantification to enable the calculation of the concentration of DNA sequences in the initial sample. (denovix.com)

Alternative non-destructive methods based on chlorophyll fluorescence require expensive equipment and are less suitable for multiple sampling of small cultures which need to be maintained under axenic growth conditions. (frontiersin.org)
Since the fluorescence intensity of rare events and the position of thousands of surrounding cells are recorded at the same time, the method associates the advantage of the FCM analysis and the microscopic observation. (nih.gov)

A method for quantitative analysis of eight exogenous eCB internal standards (anandamide-d4, docosahexaenoyl ethanolamide-d4, eicosapentaenoyl ethanolamide-d4, linoleoyl ethanolamide-d4, oleoyl ethanolamide-d4, palmitoyl ethanolamide-d5, stearoyl ethanolamide-d3 and palmitanilide) in cerebrospinal fluid is reported here. (biorxiv.org)
Together with the "Review of EUROSTATs reporting method and statistics" published in June 2013, it serves as a basis for the development of a harmonised approach on quantitative techniques within the FUSIONS project. (eu-fusions.org)
We studied 47 patients to investigate the accuracy of coronary plaque volume measurement acquired with 64-slice multi-slice computed tomography (MSCT), using newly developed quantification software, when compared with quantitative intracoronary ultrasound (QCU). (escardio.org)
This paper provides a step-by-step protocol describing how to handle the tissue, conduct the imaging, and use stereological methods to obtain unbiased and quantitative data on fiber type-specific subcellular glycogen distribution in skeletal muscle. (jove.com)
Along with measurement of SARS-CoV-2 RNA in this step, several laboratory controls should also be measured, including matrix recovery controls, human fecal normalization, quantitative measurement controls, and controls to assess molecular method inhibition. (cdc.gov)

Here, we report the use of a targeted liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) method for concurrent quantification of multiple cytochrome P450 (P450), UDP-glucuronosyltransferase (UGT), and transporter proteins in cultured primary human hepatocytes. (aspetjournals.org)

We aim to develop a High-Performance Liquid Chromatography (HPLC) sensitive method for quantification of silibinin in aqueous samples to quantify its concentration in new formulations. (researchsquare.com)
Multiple testing methods and laboratory workflows are used to quantify SARS-CoV-2 in wastewater across the United States. (cdc.gov)

On behalf of the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM), the NTP Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM) requests public comments on nominations received for the three in vitro test methods proposed for detecting and quantifying botulinum neurotoxin (BoNT), and at second for an in vitro test method proposed for detecting non-endotoxin pyrogens. (gmp-compliance.org)
Besides the direct estimation of parasite release from infected erythrocytes, this method provides a morphological evaluation of normal infected cells approaching the end of the plasmodial life cycle, or pathological forms accumulated as the result of experimental intervention in the parasite release process. (biomedcentral.com)

There is a need to automate stochastic uncertainty quantification codes in the digital age. (polito.it)
Ensuring the reliability of Uncertainty Quantification (UQ) codes in such conditions, in an autonomous way, is a challenging problem. (polito.it)
Both methods performed within limits of allowable uncertainty based on inter- and intra-individual variation. (who.int)

Figure 2: Sensitivity and false discovery rates of differential expression methods. (nature.com)
Figure 3: Self-consistency of differential expression methods when using less data. (nature.com)

A simple, inexpensive and non-destructive method to estimate chlorophyll concentration of microalgal cultures in situ from digital photographs using the RGB color model is presented. (frontiersin.org)
The linearity of the method was established in the concentration range of 1.0-5000 ng/mL. (unboundmedicine.com)
The application of this method for routine measurement of plasma darunavir concentration was demonstrated by a bioequivalence study conducted in 40 healthy Indian subjects for a 600 mg tablet formulation along with 100mg ritonavir as booster under fast and fed conditions. (unboundmedicine.com)
The method was applied to a cohort of healthy individuals (n=33) to identify and estimate the concentration of eCBs in CSF. (biorxiv.org)
We aim to develop an HPLC-UV method, common technique for analysis of pharmaceuticals, for quantification of silibinin in the aqueous samples in order to quantification of its concentration in in vitro experiments of new formulations. (researchsquare.com)
Virus quantification is counting or calculating the number of virus particles (virions) in a sample to determine the virus concentration. (wikipedia.org)
Other methods may measure the concentration of viral proteins, DNA, RNA, or molecular particles, but not necessarily measure infectivity. (wikipedia.org)
Plaque-based assays are a commonly used method to determine virus concentration in terms of infectious dose. (wikipedia.org)
Broadly speaking, DNA quantification is any process used to determine the concentration of DNA in a sample. (denovix.com)
For DNA quantification, the intensity of these bands can be used to determine the concentration of a given DNA fragment in the initial sample. (denovix.com)
Sample processing for measuring SARS-CoV-2 RNA in wastewater involves sample preparation, sample concentration, RNA extraction, and RNA measurement methods. (cdc.gov)

Such a method of live-cell lipid quantification has (i) experimental power in basic cell biology, (ii) practical utility for identifying developmental predictive biomarkers while advancing biology-based oocyte/embryo selection, and (iii) ability to yield rationally supporting technology for decision-making in rodents, domestic species, and human assisted reproduction and/or fertility preservation. (rsc.org)

Aim(s): The aim of this study was to compare two different colorimetric methods, being Folin-Ciocalteu and DMBA assays in terms of their bias and selectivity in quantification of phlorotannin in brown seaweed. (lu.se)

We describe sleuth ( http://pachterlab.github.io/sleuth ), a method for the differential analysis of gene expression data that utilizes bootstrapping in conjunction with response error linear modeling to decouple biological variance from inferential variance. (nature.com)
The activities of transcription factors and other genes involved in cell fate decisions can be measured by a number of different gene expression quantification experiments. (lu.se)

The method does not require DNA extraction or standard curves for quantification of hr DNA in unknown samples. (scirp.org)
Surrogate analytical techniques based on chlorophyll content use solvent extraction methods for chlorophyll quantification, but these methods are destructive, time consuming and require careful disposal of the resultant solvent waste. (frontiersin.org)
Methods: Supercritical fluid extraction (SFE) was used for extraction of phlorotannins from brown seaweeds. (lu.se)
In this study, green extraction method employing supercritical CO2 was utilized, taking advantage of its less burden to the environment. (lu.se)
The method involved liquid-liquid extraction of darunavir and IS in methyl-tert-butyl ether from 50 μL human plasma. (unboundmedicine.com)
Several methods for extraction and quantification of PE are available but they comprise typically various extraction buffers, repetitive freeze-thaw cycles and liquid nitrogen, making extraction procedures more complicated. (ruc.dk)
A simple method for extraction of PE from cryptophytes is described using standard laboratory materials and equipment. (ruc.dk)
Simple method for extraction and quantification of phycoerythrin from cryptophytes. (ruc.dk)

Direct protein quantification was applied successfully to study in vitro induction in sandwich cultured primary hepatocytes in a 24-well format using the prototypical inducers rifampicin, omeprazole, and phenobarbital. (aspetjournals.org)
We conclude that targeted absolute LC-MS/MS quantification of drug-metabolizing enzymes and transporters can broaden the scope and significantly increase the impact of in vitro drug metabolism studies, such as induction, as an important supplement or future alternative to mRNA and activity data. (aspetjournals.org)
The assessment of potential drug-drug interactions by in vitro methods has become an indispensable part of the preclinical development process of new drug candidates. (aspetjournals.org)

OBJECTIVE: The aims of this research were (a) to study the interrater reliability of a posture observation method, (b) to test the impact of different posture categorization systems on interrater reliability, and (c) to provide guidelines for improving interrater reliability. (cdc.gov)
APPLICATION: The results provide ergonomics practitioners with information about the interrater reliability ofa postural observation method and guidelines for improving interrater reliability for video-recorded field data. (cdc.gov)

Usually carried out before downstream experiments, DNA quantification is vital in ensuring the validity of experimental results. (denovix.com)

Czech Digital Mathematics Library: Quantification method of classification processes. (dml.cz)
Quantification method of classification processes. (dml.cz)

Our results indicate that insulin concentrations of one sample measured by different methods vary greatly and should be interpreted carefully. (biomedcentral.com)
Fluorometric techniques are more sensitive than absorbance-based methods such as UV-Vis DNA quantification, making them more suitable for measuring low concentrations of DNA and are more specific for the analyte of interest. (denovix.com)

In this paper we develop a method that applies these principles to a particularly hot problem in cell biology, the study of stem cell like cells in cultures of primary cells, cancer cells, and various cell lines. (nih.gov)
This study aimed to compare non-sedated free-breathing multi-echo 3-D stack-of-radial (radial) MRI versus standard breath-holding MRI and SVS techniques in a group of children for fat quantification with respect to image quality, accuracy and repeatability. (springer.com)
In the current study therefore, recently developed dedicated quantification software was evaluated against intravascular ultrasound (IVUS)(3). (escardio.org)
This study facilitated the quantification of collagen and understanding of the collagen constituents in sea cucumber . (bvsalud.org)

ABSTRACT We aimed to develop a reliable, low cost method to assess the early stages of renal impairment in diabetes, for use in high-risk populations in countries with limited resources. (who.int)

We show that the ddPCR method has acceptable precision and high accuracy, works with different biologic drugs, and compared to qPCR shows higher tolerance of drug matrices. (scirp.org)

The FFA method typically yields results in less time than plaque assays or fifty-percent-tissue-culture-infective-dose (TCID50) assays (see below), but it can be more expensive in terms of required reagents and equipment. (wikipedia.org)
Typically, these dyes (such as PicoGreen or SYBR Green) are specific to double-stranded DNA, while UV-Vis DNA quantification methods don't discriminate between RNA and DNA. (denovix.com)

Further improvement in CT resolution is required for more reliable measurement of coronary plaques using quantification software. (escardio.org)
Conclusions: 3DVUS using the volumetric-linear array method accurately measures plaque volumes, including those of small plaques. (itg.be)

Current T1 measurement methods are time-consuming. (tue.nl)

Therefore, an exact quantification of insulin by laboratory analysis is the most important and challenging step to diagnose EMS-related changes in insulin regulation. (biomedcentral.com)

The sensitivity of the developed method was 10 µg/ml. (researchsquare.com)
The clinical sensitivity and specificity of the TCA method determined against this ratio were 86% and 90% respectively. (who.int)

By following the methods described here it should be possible to produce a standard curve for chlorophyll analysis in a wide range of testing conditions including different microalga cultures, culture vessel and photographic set up in any particular laboratory. (frontiersin.org)
Moreover, the recent report on frequent artifacts on real-time PCR results [ 33 ] has raised the questions of validity of the existing DNA-based methods in the analysis of highly processed meat products. (hindawi.com)
Standard test methods for analysis of selected elements in waterborne oils. (cdc.gov)

To consider a broad range of different scenarios for binary as well as multiclass quantification settings, we carried out almost 3 million experimental runs on 40 data sets. (arxiv.org)

The method is used here to show that swelling of schizonts caused by protein-free media inhibits parasite release. (biomedcentral.com)

They seek data generated through alternative test methods for detecting and quantifying BoNT, including but not limited to three test methods nominated by BioSentinel Pharmaceuticals, Inc. (BioSentinel). (gmp-compliance.org)
In addition, the department is looking for data generated through alternative test methods for identifying non-endotoxin pyrogens, including but not limited to the monocyte activation test (MAT), which was nominated by Biotest AG. (gmp-compliance.org)
FUSIONS has also published a "Food Waste Quantification Manual" to support EU Member States (MS) in establishing more reliable monitoring and reporting of national food waste data at each stage in the food supply chain. (eu-fusions.org)
The Manual can be used as a reference by researchers and Member State authorities to develop coherent methods for acquiring food waste data in order to fill in data gaps more effectively. (eu-fusions.org)
Laboratory controls can ensure that results are comparable by accounting for method performance and data quality. (cdc.gov)
Testing methods include sample processing steps, use of laboratory controls, and implementation of biosafety measures to ensure that data can be interpreted for public health use. (cdc.gov)

NICEATM received nominations for validation studies on each of the above test methods, which have the potential to reduce or replace animal use for regulatory testing. (gmp-compliance.org)
Validation parameters for establishing robust standardised methods for cannabinoid quantification within Cannabis-based drug forms are critically discussed. (researchgate.net)
This research aimed at the development and validation of an ultra-performance liquid chromatography (UPLC) method for the quantification of glutathione (GSH) in grape juice and in white wine after derivatisation with para-benzoquinone. (unimi.it)

Our results can guide practitioners who intend to apply quantification algorithms and help researchers to identify opportunities for future research. (arxiv.org)

Each method has its own advantages and disadvantages, which often determines which method is used for specific applications. (wikipedia.org)

Attin T, Wegehaupt FJ: Methods for assessment of dental erosion. (karger.com)

The low requirement for equipment (i.e., a simple digital camera, available on smartphones) and widely available standard software for measuring pixel intensity make this method suitable for both laboratory and field-based work, particularly in situations where sample, qualified personnel and/or equipment is limited. (frontiersin.org)

The quantification of parasite release without any contribution of parasite invasion is the prerequisite for studying parasite release from host cells, a largely unexplored aspect of parasite biology. (biomedcentral.com)

The method developed is performed in conjunction withthe information provided by the Swedish environmental agency (Svenskanaturvårdsverket, SNV) in regards to PCB limits for sensitive land usage. (essays.se)

Specific and sensitive ultra-high performance liquid chromatography-quadrupole time of flight-mass spectroscopy (UPLC-QTOF-MS) methods have been developed for the determination of curcuminoids and curcumin metabolites in human blood plasma. (nih.gov)

Background: Colorimetric methods such as Folin-Ciocalteu (F-C) and 2,4- dimethoxybenzaldehyde (DMBA) assays have been widely used for total phlorotannins content (TPC) estimation. (lu.se)
However, when assessing the bias between the two methods using spiked Ulva lactuca extract, the F-C and DMBA assays exhibited biases of 4.2 ± 1.0 % and -3.04 ± 1.1 %, respectively. (lu.se)
Although both assays showed good trueness in phlorotannin's quantification, a big difference in TPC acquired by these assays was observed. (lu.se)

A simple, precise and rapid ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method has been developed and validated for the quantification of darunavir, a protease inhibitor, using darunavir-d9 as internal standard (IS). (unboundmedicine.com)
B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 879 IS - 24 N2 - A simple, precise and rapid ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method has been developed and validated for the quantification of darunavir, a protease inhibitor, using darunavir-d9 as internal standard (IS). (unboundmedicine.com)

Here we describe development of a direct E. coli hr DNA dd PCR method where the drug is directly added to the ddPCR reaction. (scirp.org)
Patients with cardiorespiratory disease use a variety of terms to describe the experience of being breathless, and it has been proposed that dyspnoea descriptors may provide a direct route for its quantification. (bmj.com)

More generally, we find that the performance on multiclass quantification is inferior to the results obtained in the binary setting. (arxiv.org)

Fluorometric DNA quantification techniques rely on the use of molecular dyes, which preferentially bind to DNA molecules. (denovix.com)
Methods in molecular biology (Clifton, N.J. (lu.se)

In order to address potential barriers to analytical method standardisation, a collaborative research initiative inclusive of academic and commercial stakeholders is proposed. (researchgate.net)

The aim of this paper is to document the development of a method for detectionand quantification of Polychlorinated biphenyls (PCB) in soil using GasChromatography (GC) connected to a Quadrupole Mass Spectrometer (MS) via aninternal standard (CB189). (essays.se)

J. Tchuindjang Tchoufang , H. Paydas , N. Hashemi , O. Dedry & J. Lecomte-Beckers , «Development of quantification methods applied to HSS alloys for carbides volume fraction and grain size assessments», Acta Stereologica [En ligne], Proceedings ICSIA, 14th ICSIA abstracts, URL : https://popups.ulg.ac.be/0351-580x/index.php?id=3845. (ac.be)

The method is highly reproducible, easy to perform, and does not require expensive equipment. (biomedcentral.com)

Anatomy12

Organisms9

Diseases2

Chemicals and Drugs47

Analytical, Diagnostic and Therapeutic Techniques and Equipment137

Phenomena and Processes32

Disciplines and Occupations14

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture10

Information Science19

Health Care28

Detection10

Assay3

Accurate9

Amplification3

Fluorescence2

Quantitative5

Coupled to tandem mass spectro1

Quantify2

Evaluation2

Uncertainty3

Differential expression2

Concentration11

Lipid1

Selectivity1

Gene2

Extraction8

Vitro3

Observation2

Experiments1

Classification2

Concentrations2

Study4

Abstract1

QPCR1

Typically2

Plaques2

Measurement1

Laboratory analysis1

Sensitivity2

Analysis3

Experimental1

Protein1

Data6

Validation3

Researchers1

Advantages1

Assessment1

Suitable1

Contribution1

Limits1

Determination1

Assays3

Chromatography2

Direct2

Results1

Molecular2

Analytical method1

Mass spectrometer1

Development1

Reproducible1