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  • cell
  • We found that conjugation of cell penetrating peptide (CPP) to PNA is essential for the antimicrobial effect, with CPP1 [(KFF)3K] being the most effective against E. amylovora . (frontiersin.org)
  • polyamide
  • Earlier, we have shown that an anti-TAR polyamide nucleotide analog (PNA TAR ) conjugated to a membrane transducing (MTD) peptide, transportan, is efficiently taken up by the cells and displays potent antiviral and virucidal activity [B. Chaubey, S. Tripathi, S. Ganguly, D. Harris, R. A. Casale and V. N. Pandey (2005) Virology , 331, 418- (pubmedcentralcanada.ca)
  • Synthesis
  • Methods, employing a polycyclic hydrocarbon or a polycyclic heteroaromatic compound as sensitizers, are provided to increase the efficiency of removing, by irradiation, photolabile protecting groups that mask reactive sites on synthesis intermediaries. (google.com)
  • Pipkorn R, Wiessler M, Waldeck W, Hennrich U, Nokihara K, Beining M, Braun K. Improved Synthesis Strategy for Peptide Nucleic Acids (PNA) appropriate for Cell-specific Fluorescence Imaging. (medsci.org)
  • In this context our modified solid phase peptide chemistry using temperature shifts during synthesis is considered as an appropriate technology. (medsci.org)
  • Contributions were made by the introduction of the Merriefield's solide phase peptide synthesis [ 8 ] and Carpino's protecting group methodologies [ 9 ]. (medsci.org)
  • Contemporary approaches to the synthesis of chemically modified biomacromolecules (proteins, nucleic acids, lipids, and carbohydrates) not only require efficient means to control conjugation and the specific site of attachment of the conjugated moiety but also the effective use of recent developments in the fields of pharmaceutical chemistry, biomolecular/polymer engineering, and nanobiotechnology. (worldcat.org)
  • In this second edition of Bioconjugation Protocols: Strategies and Methods, expert researchers update the classic methods and introduce valuable new approaches that go beyond basic conjugation techniques to include elements from advanced organic synthesis, molecular biology, surface biotechnology, materials science, and nanobioscience/engineering. (worldcat.org)
  • enumeration
  • Current methods for identification and enumeration take 1-2 weeks and rely on semi-selective culture medium followed by final identification from morphology and biochemical testing. (usda.gov)
  • droplet
  • 2 . A method according to claim 1 , wherein the step of dispensing droplets includes limiting each droplet to a specified volume smaller than one microliter. (google.com)
  • 3 . A method according to claim 1 , wherein dispensing each droplet onto the surface includes dispensing each droplet while the surface is moving. (google.com)
  • 6 . A method according to claim 1 , wherein moving the surface through the delay line includes hanging each droplet beneath the surface. (google.com)
  • 8 . A method according to claim 1 , further comprising a step of analyzing a characteristic of each droplet. (google.com)
  • 13 . A method according to claim 9 , wherein tracking each droplet's position includes measuring and recording each droplet's position on the moving surface using a position sensor, such that each droplet is associated with a fiducial position on the moving surface. (google.com)
  • 15 . A method according to claim 13 , wherein the steps of measuring and recording occur at substantially the same time each droplet is dispensed onto the moving surface. (google.com)
  • probe
  • Methods for preparing a biochip are provided herein wherein the biomolecular probe to be used with the biochip is alternatively bound to a hydrogel prepolymer prior to or simultaneously with polymerization of the prepolymer. (google.com)
  • In particularly preferred embodiments, a polyurethane-based hydrogel prepolymer is derivatized with an organic solvent soluble biomolecule, such as a peptide nucleic acid probe in aprotic, organic solvent. (google.com)
  • virucidal
  • These results indicate that anti-HIV-1 PNA conjugated with MTD peptides are not only inhibitory to HIV-1 replication in vitro but are also potent virucidal agents which render HIV-1 virions non-infectious upon brief exposure. (pubmedcentralcanada.ca)
  • sequence
  • 3. The method of claim 1 wherein at least a portion of the oligonucleotide is complementary to a portion of the target sequence intervening the upstream and downstream primers. (freepatentsonline.com)
  • Methods for amplifying genes requiring only one gene-specific primer impose less sequence-dependent bias than standard two-primer PCR amplification procedures. (asmscience.org)
  • compositions
  • There is a need in the art for improved procedures for analyzing samples, and for compositions and articles of manufacture useful in such methods. (google.com)
  • There is disclosed compositions and methods relating to or derived from anti-PSMA antibodies. (patents.com)
  • carrier
  • After ligation to the modular peptide-based BioShuttle carrier, which was utilized to facilitate the delivery of the functional modules into the cells' cytoplasm, the modules were scrutinized. (medsci.org)
  • analysis
  • Using microscopy and molecular community analysis techniques, the effects of antifouling methods will be assessed. (southampton.ac.uk)
  • Limitations on the amount of diagnostic information that can be obtained from a sample include the size of the sample that can be obtained and readily manipulated, the processing time required to perform multiple tests, the tolerance of the sample for multiple treatment steps without loss of signal, and the cost for performing multiple methods of analysis. (google.com)
  • infection
  • The rate of new infection depicts the failure of existing and approved methods to control the spread of the deadly virus. (pubmedcentralcanada.ca)
  • This 36mer peptide directed against the HIV-1 glycoprotein gp41 ( 3 ) is administered by subcutaneous injection and is currently the only AIDS drug which exerts its action outside the target cell prior to infection. (pubmedcentralcanada.ca)
  • comprises
  • The method comprises drawing a liquid sample transversely through a filter using an automated device that can control the rate of flow through the filter. (google.com)
  • identification
  • said composition thereby permitting the separate identification of two or more chemiluminescent labels in a single test sample as an indication of the presence of said nucleic acid analytes in the test sample. (sumobrain.com)
  • potential
  • This project will assess the cleanliness of existing endoscopes and the efficacy of current decontamination procedures, using a new highly sensitive microscopy imaging method to identify and quantify residual contamination from a range of flexible endoscopes, and new cell assays to assess potential prion infectivity. (southampton.ac.uk)
  • molecular
  • Methods: In this paper I tested by laboratory examination and literature/patent searches whether 40 Inventive Principles were used in molecular genetics laboratories and whether it is possible to derive post hoc Primary Parameter and Conflicting Parameter of the Contradiction Matrix for these Principles. (triz-journal.com)