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  • genomic
  • To obtain a maximally completed sequence, three libraries are cloned into the phage vector, M13, bacteriophage are used: with the 0.5 kb and 7 kbp insert consisting of two sequences, with the average distance in genomic DNA of 100 kbp. (google.com)
  • Also disclosed are corresponding genomic-fragment clones containing polynucleotides encoding the open reading frame sequences for the antigenic peptides. (freepatentsonline.com)
  • A general method for screening genomic or cDNA, or fragments and mixtures thereof, involves sample simplification by the generation of subsets and then subjecting the subsets to a modified mismatch scanning procedure that eliminates DNA having single stranded breaks after a MutSLH cleavage. (google.com)
  • The methods are particularly useful in human population isolates, including identification of identical-by-descent sequences, genomic comparisons of two or more individuals, and genomic comparisons of two populations of individuals, for the identification of sequences of low polymorphism. (google.com)
  • The National Human Genome Research Institute (NHGRI) has continued its coordinated effort to support the development of technologies to dramatically reduce the cost of DNA sequencing, a move aimed at broadening the applications of genomic information in medical research and health care. (genome.gov)
  • Abstract
  • Abstract DESCRIPTION (provided by applicant): The rationales for the development of technology that will enable extremely cheap, high speed sequencing are well established. (sbir.gov)
  • nucleotide
  • 2 . The method of claim 1 further comprising removing nucleotide derivatives and nucleotides that are not incorporated into the primer. (google.ca)
  • 3 . The method of claim 2 further comprising the step of repeating said conducting and removing steps using a second nucleotide species and a nucleotide derivative corresponding to a nucleotide species different from said second species. (google.ca)
  • The invention provides methods for correcting misincorporation of a nucleotide in a primer during a sequencing-by-synthesis reaction by using both a polymerase substantially lacking in exonuclease activity and an enzyme, preferably a polymerase, having exonuclease activity. (google.es)
  • synthesis
  • Whereas we have made significant progress in demonstrating the power of digital microfluidics in sequencing by synthesis in our current R21 proposal, some key experimental aspects of the technology as applied to achieving long reads need to be demonstrated. (genome.gov)
  • 5) experimentally determine read length limitations in droplet-based sequencing by synthesis, and implement software and signal processing strategies to improve read lengths and data quality and throughput, with a goal to demonstrate 1,000 to 10,000 base pair reads by Year 3. (genome.gov)
  • loci
  • Targeted sequencing of the specific loci associated with a complex trait in large numbers of individuals is a promising approach for using current sequencing technologies to identify and characterize the variants in these intervals. (biomedcentral.com)
  • chemistry
  • Miniaturized arrays may be used in a variety of applications, such as gene sequencing, monitoring gene expression, gene mapping, bacterial identification, drug discovery, and combinatorial chemistry. (google.com)
  • target
  • Although PCR enriches target sequences with high specificity and sensitivity, it is difficult to scale the method. (biomedcentral.com)
  • a) Can flexible 'molecular wires' bridge the gap between sensing electrodes and the target attachment sites on the DNA to be sequenced? (genome.gov)
  • 2. The method of claim 1 further comprising the step of automatically analyzing each frame of said mosaic of video images based upon target cell selection criteria and automatically selecting target cells of interest based upon said target cell selection criteria. (google.com.au)
  • 4. The method of claim 2 further comprising the steps of manually selecting additional target cells of interest or manually deselecting target cells automatically selected based on said target cell selection criteria. (google.com.au)
  • The invention involves a novel method and apparatus for obtaining pure cell populations or cell constituents such as DNA, RNA or proteins from target cells in tissue sections using ultraviolet (UV) laser-assisted ablation of non-target cells. (google.com.au)
  • Bases
  • No. 60/103,998, entitled "On the Power of Universal Bases in Sequence by Hybridization" and filed Oct. 13, 1998 and U.S. application Ser. (google.com)
  • When combined with a nanopore-DNA translocation system that presents each base sequentially to the electronic sensor, it appears that at least 100 million bases per day could be read with continuous sequence runs of at least 80,000 bases. (genome.gov)
  • arrays
  • Methods of manufacturing high-density arrays of reactants. (google.com)
  • This invention relates to arrays manufactured on polymeric surfaces and more particularly to high-density, miniaturized arrays and methods of manufacturing the same. (google.com)