Methods for extraction of dna of fungal pathogens. Medical search. Frequent questions

ABSTRACT We evaluated the utility of 2 methods for detection of methicillin-resistant Staphylococcus aureus (MRSA) directly from signal-positive blood culture bottles: loop-mediated isothermal amplification (LAMP) assay, and direct cefoxitin disk diffusion (DCDD) test using a 30 g cefoxitin disk. (who.int)

Dr. Hamprecht is a clinical microbiologist at the Institute for Medical Microbiology, Immunology and Hygiene and professor for antibiotic resistance of gram-negative pathogens at the University of Cologne, Germany, and the German Centre for Infection Research (DZIF), also in Cologne. (cdc.gov)
Enteric pathogens can cause gastrointestinal infection and may be included in this group. (wikipedia.org)
In this study, a suppression subtractive hybridization cDNA library approach was used for the identification of pathogen and plant genes that were differentially expressed during infection of the susceptible cultivar BRS Pérola of Phaseolus vulgaris L. A total of 979 unigenes (430 contigs and 549 singletons) were obtained and classified according to their functional categories. (frontiersin.org)
Pneumocystis pneumonia (PCP) is a common and serious opportunistic infection in immunocompromised patients that is caused by the fungal pathogen Pneumocystis jiroveci , formerly known as P. carinii f.sp. (biomedcentral.com)
Fungal infection of roots can occur at any time during the production cycle, while colonization of flower buds generally occurs during the later stages of flower development and can be manifested as a pre-harvest or post-harvest bud rot. (cannaqa.wiki)
The aim of this study was to characterise the impact of a resistance gene against L. maculans infection on residue microbial communities and to identify micro-organisms interacting with this pathogen during residue degradation. (biorxiv.org)
Measures to combat fungal infections require detailed knowledge of the fungal infection biology. (biomedcentral.com)
Large-scale genomics and transcriptomics approaches have been proven to enable the identification of pathogenicity genes in fungi affecting humans or plants [ 8 ], which makes them valuable tools towards a better understanding of fungal infection mechanisms. (biomedcentral.com)
The pathogen infection mechanism, however, remains elusive. (biomedcentral.com)
Microbial bio control agents are now one of the widely used approach in which microbial species are used to eliminate or inhibit the growth of pathogens in order to reduce the severity of the infection. (springeropen.com)
These chemicals are classified as bactericides, fungicides and nematicides which suppress the plant infection caused by bacteria, fungal and nematodes, respectively. (springeropen.com)
Conventional hospitals in Egypt to implement sound 1 mL aliquot was used directly for the methods for the identification of S. au- infection prevention and control pro- LAMP assay and DCDD test. (who.int)
Candidiasis (candidosis) is by far the most common fungal infection of the mouth (oral cavity). (medscape.com)
therefore, a diagnosis of a deep-seated fungal oral infection should prompt the clinician to investigate systemic involvement as well as to determine the integrity of the immune system of the affected individual. (medscape.com)
The most common presentation of oral deep fungal infection is a chronic, solitary ulcer or nodule. (medscape.com)

In this study, we isolated culturable endophytic bacteria and fungi from soybean seeds and evaluated their antagonistic activities against common bacterial and fungal seed-borne pathogens of soybean. (ppjonline.org)
Enterobacterales and fungi), their resistance mechanisms, and the improvement of diagnostic methods. (cdc.gov)
In this study, six qPCR assays were compared in five independent laboratories using the same set of DNA samples from fungi, plants, and soil. (illinois.edu)
Fungal community structure differed between the two lines at harvest, but not subsequently, suggesting that the presence/absence of the resistance gene influences the microbiome at the base of the stem whilst the plant is alive, but that this does not necessarily lead to differential colonisation of the residues by fungi. (biorxiv.org)
In addition to its economical importance, C. graminicola is among the best characterized and most tractable fungi of the genus Colletotrichum , a genus comprising a broad range of hemibiotrophic plant pathogens that represent a constant threat to fruit and vegetable production worldwide ( http://www.broadinstitute.org ). (biomedcentral.com)
Moreover, co-occurrence interactions between fungi and bacteria, mostly contributed by fungal Ascomycota and bacterial Proteobacteria in control, were reverted to co-exclusive interplay in CRC (p=0.00045). (bmj.com)
An increasing number of infections, including zoonoses, lack of taxonomic stability and ambiguous clinical picture of all cases of dermatomycosis induce to search for new, fast, repeatable and at the same time cheap methods of species identification of these fungi. (am-online.org)
The results of many studies indicate that the direct identification of fungi from dermatological samples based on molecular methods is much more reliable and much faster compared to that carried out by conventional methods. (am-online.org)
Particular molecular methods used in the species identification of fungi directly from the clinical material differ in the procedures of genomic DNA extraction, PCR techniques used, the molecular marker used and the results interpretation system. (am-online.org)
The University of Guelph, Agriculture & Food Laboratory offers soil nutrient and diagnostic services providing micro and macro nutrient, fertility analysis as well as detection of soil pathogens such as fungi, nematodes, etc. (uoguelph.ca)

The cause opportunistic invasive fungal infections. (cdc.gov)
Descritores: Levedura, diagnóstico molecular, extração de RNA, RT-PCR 1 INTRODUCTION Advances in Medicine have led to extended survival of immunocompromised patients and increased importance of opportunistic fungal infections (1-2). (livrozilla.com)
We now report our seminal findings on the major constituents including terpenes identified in native, historically significant herbal medicinal plant Elder (Sambucus nigra L.) flower and elder berry in particular and their concomitant strong antimicrobial effects exhibited on various nosocomial pathogens notably upon methicillin-resistant Staphylococcus aureus MRSA, recognised globally as a clinically significant pathogen, associated with skin and soft tissue infections. (researchgate.net)
1-3 Unlike bacterial infections, we do not have much choice of medications in fungal infections. (eophtha.com)
Patients are usually treated as bacterial infections and would have received steroids before they are diagnosed to have fungal endophthalmitis. (eophtha.com)
The present disclosure provides for methods of diagnosing and treating bacterial infections. (warf.org)
This yeast is also the major opportunistic pathogen responsible for both superficial and disseminated infections. (springer.com)
Fungal infections of the skin, hairs, and nails undeniably dominate among all types of fungal infections. (am-online.org)
The etiological factors of the majority of superficial fungal infections are dermatophytes which, although they are the oldest microorganisms considered as pathogens, have long been unstable in the taxonomic position. (am-online.org)
In the last decade, revolutionary progress has been observed in the development of molecular methods for the diagnosis of fungal infections and the reliable identification of species of etiological factors that cause these dermatomycoses. (am-online.org)
Superficial fungal infections are common in dermatology and are often caused by opportunistic species in the Candida and Malassezia genera. (actasdermo.org)
This article focuses on noncandidal oral fungal infections (deep mycoses). (medscape.com)
The oral lesions associated with these deep fungal infections are chronic and progress to form solitary, chronic deep ulcers with the potential for local destruction and invasion and systemic dissemination. (medscape.com)

A common effect of these fermentations is that the food product is less hospitable to other microorganisms, including pathogens and spoilage-causing microorganisms, thus extending the food's shelf-life. (wikipedia.org)
The traditional culture-based method is time-consuming (taking days or even weeks), especially some microorganisms are unculturable in a lab setting. (quickbiology.com)

2(4):308-10 Detection of clinically relevant yeast in blood: evaluation of methods for RNA extraction and amplification In this paper, we compare different methods for RNA extraction from yeast cells spiked in blood and different reagents for enzymatic amplification. (livrozilla.com)
Detection of Pneumocystis-DNA was associated with a worse prognosis: seven (44%) of patients with positive PCR died within one month compared to nine (14%) of the controls (p = 0.01). (biomedcentral.com)
In order to increase sensitivity in non-invasive specimens, several experimental studies have evaluated PCR detection of P. jiroveci DNA as a potential diagnostic test. (biomedcentral.com)
Multicopy gene-based assays targeting the ribosomal DNA intergenic spacer (IGS) or the mitochondrial small subunit (mtSSU) showed relatively high sensitivity (limit of detection [LOD] = 0.05 to 5 pg) compared with a single-copy gene (FvTox1)-based assay (LOD = 5 to 50 pg). (illinois.edu)
Detection of bacterial DNA has been investigated as a marker of SBP and as a predictor of prognosis. (frontiersin.org)
Research & Innovation (R&I) activities will be focused on developing low-cost paper-based isothermal NATs, their integration into microfluidic devices and optimization for use in a) tissue engineering b) agrifood (poultry/plant/fungal) pathogen detection c) detection of toxigenic freshwater Cyanobacteria causing harmful algal blooms. (wbc-rti.info)
To minimize these losses, early detection of these pathogens is needed. (ualberta.ca)
RT-iPCR resulted in a limit of detection of 1, 188, and 938 spores for Ptr, Pst, and Fg respectively without involving complex procedures of DNA extraction as this method directly measures the spores. (ualberta.ca)
qPCR resulted in a limit of detection of 3, 400, and 800 spores for Ptr, Pst, and Fg respectively after being corrected to DNA extraction efficiency. (ualberta.ca)
These techniques would help in early detection of fungal spores and prevents the spread of plant diseases. (ualberta.ca)
Minimal Residual Disease (MRD) detection assays such as those used to detect the presence of cancer at early stages have largely relied on single nucleotide variant (SNV) detection in plasma DNA. (warf.org)
The current gold standard method for COVID-19 testing is a multi-step protocol 25 involving RNA extraction using column-based or magnetic bead-based methods, followed by RT-qPCR-based detection of the. (warf.org)
In Nature Medicine , Dr. Charles Chiu in UCSF-Abbott Viral Diagnostics and Discovery Center ( VDDC ) describes a simple, rapid, and universal method of pathogen detection by metagenomic next-generation sequencing ( mNGS ) analysis of cell-free DNA ( cfDNA ) from a variety of different body fluids, ranging from low-cellularity cerebrospinal fluid ( CSF ) to purulent fluids with high human host DNA content ( ref1 ). (quickbiology.com)
To evaluate their mNGS method sensitivity and precision, they collected 182 samples from 160 patients, performed cultured based pathogen detection, 16S ribosomal RNA, and 28S-ITS PCR assays in parallel ( Table 1 ). (quickbiology.com)

Candida albicans is the most important opportunistic fungal pathogen of humans. (ncl.edu.tw)

A total of 87 bacterial isolates and 66 fungal isolates were obtained. (ppjonline.org)
A total of 465 fungal isolates were obtained from a total of 358 isolated plant samples. (online-rpd.org)

Our antibacterial and antifungal activity assay showed that four fungal species and nine bacterial species have the potential to suppress the growth of at least one seed-borne pathogen tested in the study. (ppjonline.org)
de Bary, is a necrotrophic pathogen with worldwide distribution and is known to infect over 400 species of plants ( Boland and Hall, 1994 ). (frontiersin.org)
The pathogens affecting production of hemp have been described and include fungal, bacterial, viral, and nematode species. (cannaqa.wiki)
This approach could be used to identify and promote micro-organisms with beneficial effects against residue-borne pathogens, and more broadly, to decipher the complex interactions between multi-species pathosystems and other microbial components in crop residues. (biorxiv.org)
To date, chytridiomycosis has decimated over 500 amphibian species, representing the greatest loss of biodiversity due to a pathogen ever recorded [ 4 ]. (biomedcentral.com)
In addition, candidiasis is a zoonotic disease, therefore pathogens originating from different sources may be transferred to other host species [ 4 ]. (springer.com)
The cucurbit powdery mildew (CPM) caused by different fungal species is a major concern for cucurbit crops around the world. (ppjonline.org)
Some of them are developed earlier but there is still need to develop more pathogen-resistant species for example in case of silencing of genes with insertion of a viral segment. (springeropen.com)
Aim: To measure the agreement of methods for identification of Candida species in oral cavity samples, comparing the CHROMagar Candida, microculture, API 20C AUX and RAPD techniques. (bvsalud.org)
There is a variety of methods for identifying Candida species from clinical samples in the oral cavity 5 . (bvsalud.org)
Methods based on molecular markers are useful not only for phenotyping, but also for differentiation of Candida species 15-16 . (bvsalud.org)
DNA probes are available for several species. (medscape.com)

Due to the growing concerns about environmental damage and the development of resistance to agrochemicals among pathogen populations, there is a strong demand for eco-friendly alternatives to synthetic chemicals in agriculture. (ppjonline.org)
This work showed that a seed-endophytic bacterium Sphingomonas melonis can confer resistance to the seed-borne pathogen Burkholderia plantarii via the production of anthranilic acid, which interferes with the sigma factor RpoS of the pathogen. (ppjonline.org)
Our research focuses primarily on isolate variability, host resistance and pathogen biology. (illinois.edu)
Fun(gi)omics: Advanced and Diverse Technologies to Explore Emerging Fungal Pathogens and Define Mechanisms of Antifungal Resistance. (cdc.gov)
Under natural pathogen pressure, inoculation with strain RMB7 and RMB7-supernatant conferred resistance by arugula plant against Pythium irregulare with a concurrent growth improvement over non-inoculated plants. (biomedcentral.com)
Earlier, on the basis of the natural phenomenon, it was assumed that evolution was the only process to deal with pathogens in which plants themselves undergoes the process of evolution either they develop resistance or sensitivity to the pathogens attacked. (springeropen.com)

Pea- an important cash crop, rich in nutrients, but frequently infected with powdery mildew (fungal disease caused by Erysiphe pisi ) that destroys the whole crop and causes economic loss for growers. (nature.com)
On the foliage, powdery mildew , caused by Golovinomyces cichoracearum , was the major pathogen observed. (cannaqa.wiki)

Fusarium equiseti is a plant pathogen with a wide range of hosts and diverse effects, including probiotic activity. (researchsquare.com)
Root-infecting pathogens included those from the Fusarium genus ( Fusarium oxysporum , Fusarium solani , and Fusarium brachygibbosum ) and the Pythium genus ( Pythium dissotocum , Pythium myriotylum , and Pythium aphanidermatum), which caused root browning, discoloration of the crown and pith tissues, stunting and yellowing of plants, and in some instances, plant death. (cannaqa.wiki)
Sudden death syndrome (SDS) of soybean is caused by a soilborne pathogen, Fusarium virguliforme . (illinois.edu)
These isolates were identified as F. avenecaum, F. culmorum, F. graminearum and F. croockwellense Fusarium graminearum was the predominant pathogen isolated, followed by F. culmorum F. avenecaum and F. croockwellense , respectively. (scialert.net)

The transcriptional profile of 11 fungal genes related to pathogenicity and virulence were evaluated by reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). (frontiersin.org)
The role of the microbiota in the plant's response to a disease or in the pathogenicity of a fungal pathogen is currently being studied in various pathosystems, but remains poorly understood. (biorxiv.org)
Here we present the first reference genome and transcriptome of C. personatum to support studies in phylogenetics, genetic diversity, pathogenicity, and metabolites of this peanut pathogen. (biomedcentral.com)

Aerial distribution of pathogen inoculum and mold spores and dissemination through vegetative propagation are important methods of spread, and entry through wound sites on roots, stems, and bud tissues facilitates pathogen establishment on Cannabis plants. (cannaqa.wiki)
DNA extraction efficiency determined for Fg, Pst and Ptr spores are 5%, 14% and 290% respectively. (ualberta.ca)
in the amplification of DNA isolated from single yeast colonies and fungal spores using REDExtract-N-Amp ™ seed PCR kit. (sigmaaldrich.com)

A polymerase chain reaction (PCR) assay using primers for the internal transcribed spacer region (ITS) of ribosomal DNA confirmed identity of the cultures. (cannaqa.wiki)

Moreover, the traditional methods for functional gene discovery are insufficient to meet the modern-day research demands due to the high error rates and low efficiencies. (researchsquare.com)
Hence, we used quantitative real-time PCR (qRT-PCR), Illumina MiSeq (ITS rRNA) and FUNGuild to examine ITS rRNA gene populations, soil fungal succession and their functions under control (CK) and sugarcane straw retention (SR) treatments at different soil layers (0-10, 10-20, 20-30, and 30-40 cm) in fallow fields. (biomedcentral.com)
In a subset of 10 representative patients, bacterial DNA was extracted from ascites and whole blood, followed by 16S rRNA gene amplicon sequencing. (frontiersin.org)
All isolates were identified as D. citri by analyzing the sequences at the internal transcribed spacer (ITS) rDNA region using primers of ITS1/ITS4 or at β-tubulin using primer Btdcitri-F/R. Therefore, based on the present study, where the results of morphological identification of conidial type were consistent with DNA sequence analysis of certain gene, choosing a suitable method for a fast diagnosis of citrus melanose was suggested. (online-rpd.org)
Microbiome taxonomic and functional profiles were assessed using high-throughput bacterial 16S rRNA and fungal ITS2 gene sequencing, bacterial shotgun metagenomics and skin mucosal metabolomics. (biomedcentral.com)
Histone-modifying enzymes, which regulate eukaryotic chromatin conformation and gene expression, are key epigenetic factors controlling fungal development, virulence, and secondary metabolism. (biomedcentral.com)
Fungal pathogenesis is a sophisticated process including infectious development of the pathogen and fungus-host interactions, which requires a fine-tuned regulation of gene expression dynamics. (biomedcentral.com)
NCBI-GenBank lists only five gene fragments, a total of 3534 bp as follows: 1122 bp of DNA dependent RNA Polymerase II second largest subunit, 1069 bp of rRNA subunits/ITS regions, 451 bp of calmodulin, 354 bp of histone-H3, 321 bp of elongation factor 1-alpha and 217 bp of the actin gene. (biomedcentral.com)

Methods: Different combinations of commercially available kits were tested with blood samples artificially inoculated with yeast cultures. (livrozilla.com)
Collection and extraction of occupational air samples for analysis of fungal DNA. (cdc.gov)
Metagenomics is the study of genetic material from environmental samples for detecting microbes, especially pathogens. (warf.org)
UW-Madison researchers have developed and validated a novel "parasitome" method to accurately characterize parasites in samples. (warf.org)
are the pathogens most frequently cultured from CF airway samples and are associated with pulmonary decline [ 2 ]. (ersjournals.com)
Fungal colonies were isolated from samples collected in Mendoza province, Argentina. (ppjonline.org)

They commonly occur as food spoilage agents and opportunistic pathogens and are widely used as versatile cell factories. (nature.com)

Here, we analyze the epidemiology and genetic structure of natural populations of an obligate fungal pathogen, Podosphaera plantaginis . (elifesciences.org)
Oilseed rape residues are a crucial determinant of stem canker epidemiology, as they support the sexual reproduction of the fungal pathogen Leptosphaeria maculans . (biorxiv.org)

The results showed that the tested essential oils caused highly significant inhibition of fungal growth and aflatoxin production in A. flavus and A. parasiticus . (hindawi.com)
The extent of the inhibition of fungal growth and aflatoxin production was dependent on the type and concentration of essential oils applied. (hindawi.com)

Anthracnose caused by the fungal pathogen Colletotrichum lupini is the most serious disease in lupin worldwide. (cropscience.org.au)

Seed-borne pathogens in crops reduce the seed germination rate and hamper seedling growth, leading to significant yield loss. (ppjonline.org)
de Bary, one of the most important plant pathogens, causes white mold on a wide range of crops. (frontiersin.org)
The objectives of the agreement directly relate to specific project objectives in the NP301 project "Characterizing and Evaluating the Genetic Diversity and Horticultural Value of Genetic Resources for Cacao and Other Tropical Tree Crops Economically Important to the United States and in NP303 project "Developing Pathogen-and Plant-Based Genetic Tools for Breeding Disease Tolerance in Theobroma cacao. (usda.gov)
Traditionally, seed coating and mulching techniques were more common among farmers to generate better quality of the crops and prevent plants from any disease but currently new innovative methods are used. (springeropen.com)

Our goal in this work is to provide a reference genome for research on this pathogen. (biomedcentral.com)

Probiotic bacteria, including those that produce bacteriocins can kill and inhibit pathogens. (wikipedia.org)
Ethanolic extracts of Sambucus nigra L. blooms and fruits have been shown to inhibit 13 pathogens, including Staphylococcus sp. (researchgate.net)

6 h, 40-60 min are needed for nucleic acid extraction, 2-2.5 h for mNGS library preparation, 1 h for nanopore 1D library preparation and 1 h for nanopore sequencing and analysis. (quickbiology.com)

Numerous quantitative real-time polymerase chain reaction (qPCR) assays have been developed for this pathogen but their sensitivity and specificity for F. virguliforme have not been compared. (illinois.edu)

The aflatoxins are a group of chemically similar toxic fungal metabolites (mycotoxins) produced by genus Aspergillus . (hindawi.com)

Nevertheless, the gut fungal microbiota in CRC remains largely unexplored. (bmj.com)
The involvement of gut fungal microbiota in CRC is largely unexplored. (bmj.com)

Numerous agents of disease and pathogens are readily transmitted via food which includes bacteria and viruses. (wikipedia.org)
Finally, bacteriophages, viruses that only infect bacteria can be used to kill bacterial pathogens. (wikipedia.org)
A rapid method for the identification of plasmid DNA in bacteria. (microbiologyresearch.org)

Fungal colonies were morphologically diverse in shapes, aerial hyphae formation, colony, and sclerotia color. (biomedcentral.com)
Microsatellite DNA analyses of both founders and their parthenogenetically produced offspring indicated that, in both sister-pair and unrelated-pair colonies, there was no significant skew in the production of eggs, larvae, workers and soldiers. (biomedcentral.com)
As many other powdery mildews, the disease is typically recognized by the signs of the pathogen, which appear as whitish colonies in both sides of the leaves ( Fig. 1A and B ), comprising mycelium and asexual sporulation structures (conidia). (ppjonline.org)
Methods: Ninety-one colonies of Candida were isolated and presumptively identified in CHROMagar Candida, submitted to microculture, API 20C AUX and RAPD techniques. (bvsalud.org)

Thus, retaining straw to improve fungal composition, community stability and their functions, in addition to reducing soil-borne pathogens, can be an essential agronomic practice in developing a sustainable agricultural system. (biomedcentral.com)

While the ecological and evolutionary benefits of outcrossing are theoretically well-supported, support for such benefits in pathogen populations are still scarce. (elifesciences.org)
Pathogen populations supporting coinfection also have a higher probability of surviving winter. (elifesciences.org)
Identifying areas and populations where pathogens have sex can help to identify when and where new strains are most likely to emerge. (elifesciences.org)

Keywords: Yeast, molecular-based diagnosis, RNA extraction, RTPCR RESUMO Objetivos: Avaliar diferentes métodos de extração e purificação de RNA de leveduras em amostras de sangue para ser usado em diagnóstico molecular. (livrozilla.com)
Hence, extraction and purification of nucleic acids are fundamental issues in molecular-based diagnosis. (livrozilla.com)
To protect melanose in citrus farms, a fast and exact diagnosis method is necessary. (online-rpd.org)
E arly diagnosis of fungal endophthalmitis is important in order to prevent irreversible damage to the retina and prevent visual loss. (eophtha.com)

The clinical gold standard consisted of aggregated results from cultures, bacterial 16S PCR and/or fungal 28S-ITS PCR, while the composite standard also included confirmatory digital PCR with Sanger sequencing and clinical adjudication. (quickbiology.com)

We, therefore, targeted this research to find the pathogen-resistant pea lines and further decipher the diversity at er locus among resistant pea lines. (nature.com)
Overall, straw retention improved α-diversity, network structure and fungal community, while reduced soil pathogenic microbes across the entire soil profile. (biomedcentral.com)
Jointly our results show that outcrossing has direct epidemiological consequences as well as a major impact on pathogen population genetic diversity, thereby providing evidence of ecological and evolutionary benefits of outcrossing in pathogens. (elifesciences.org)
Morphological features and ISSR-based DNA markers were used to study the morphogenetic diversity. (biomedcentral.com)
The criteria to assemble this working collection will be based on (i) confirmed genetic identity through previous DNA fingerprinting, ii) geographic origin and genotypic diversity, and (iii) historical importance of the accessions. (usda.gov)

Direct interactions with other members of the community involved many fungal and bacterial ASVs (amplicon sequence variants). (biorxiv.org)

This requires that studies on the pathogens potentially affecting Cannabis plants be conducted so that methods to manage emerging diseases and molds can be developed. (cannaqa.wiki)
Two main fungal leaf spot diseases occur in peanut, namely early leaf spot (ELS) and late leaf spot (LLS), these cause a yearly average of $44 million losses. (biomedcentral.com)
Various pathogens are responsible for causing plant bacterial and viral diseases, the treatment of them is very important in order to bring out the best quality and quantity of the agricultural yield. (springeropen.com)
Various pathogens are responsible for causing plant bacterial and viral diseases, the treatment of them is very crucial. (springeropen.com)

Identification and categorization of the pathogen will be helpful in designing integrated disease management guidelines for sugar beet and dry beans of mid western America. (biomedcentral.com)
Moreover, fungal culture takes longer time and identification requires more expertise. (eophtha.com)
Bronchoscopy with bronchoalveolar lavage fluid (BALF) collection allows identification of lower airway pathogens while limiting upper airway contamination of the sample. (ersjournals.com)
To enable pathogen identification within 6h sample-to-answer time, they utilize Nanopore sequencing, a field-deployable, real-time sequencing platform. (quickbiology.com)
Whereas culture-based pathogen identification can take days to weeks, mNGS testing using nanopore or Illumina sequencing platforms has an overall turnaround time of 5-24 h. (quickbiology.com)
En parallèle, une identification microbiologique normalisée et un test de sensibilité à l'oxacilline par PCR visant l'amplification du gène Mec A ont été réalisés. (who.int)

Diverse microbes including plant pathogens are associated with seeds, and some of the microbes appear to have co-evolved alongside them ( Nelson, 2018 ). (ppjonline.org)

METHODS Strains and growth conditions Yeasts were cultivated in YPD (0.5% yeast extract, 1% peptone and 2% glucose) at 30°C and 150 rpm. (livrozilla.com)

Among them, Pseudomonas koreensis appears to have strong antagonistic activities across all the pathogens. (ppjonline.org)

METHODS: Male colorectal cancer (CRC) screenees were recruited to undergo upper endoscopy, identifying newly diagnosed cases of Barrett's esophagus. (who.int)

Many pathogens possess the capacity for sex - here defined in its broadest sense as the coming together of genes from different individuals ( Lehtonen and Kokko, 2014 ) - despite being able to reproduce also asexually and/or via selfing. (elifesciences.org)

Management of this drastic disease becomes a compulsion because the pathogen not only affected the grain and pods but also reduced pea foliage up to 33-69 per cent 5 . (nature.com)
Standard methods for fungal brood disease research. (usda.gov)
Root and stem rot caused by Rhizoctonia solani is a serious fungal disease of sugar beet and dry bean production in Nebraska. (biomedcentral.com)
[1] [2] In contrast, the pathogens affecting Cannabis have not been extensively studied, and the different growing environments, cultivation methods, as well as differences among the strains or genetic selections of hemp and Cannabis can influence disease development. (cannaqa.wiki)
Understanding the determinants of chytridiomycosis outbreak dynamics in the wild is therefore necessary to not only control disease spread and mitigate ecosystem-level impacts [ 5 ] but also elucidate the disease ecology of these super-generalist pathogens. (biomedcentral.com)
Candidiasis is a sporadic fungal disease in livestock. (springer.com)
Glomerella leaf spot (GLS) is a devastating fungal disease that damages the leaves and fruits and reduces tree vigor of apple ( Malus domestica ). (biomedcentral.com)

The prognosis of fungal endophthalmitis depends on the magnitude of intraocular involvement, the virulence of the organism, and the timing of treatment. (eophtha.com)

Cfhos2 deletion mutants induced strong plant defense responses manifested by epidermal cell browning, granulation, and distortion of pathogen invasive hyphae. (biomedcentral.com)

METHODS AND RESULTS: In this study, a total of 19 iPBS markers were used to determine the genetic relationships among 11 chestnut cultivars (Castanea sativa Mill. (bvsalud.org)
Unweighted pair group method with arithmetic averages (UPGMA) and eBURST analyses showed that the genetic construction of avian origin C. albicans population is fairly diverse. (springer.com)

2022 ). Such positive charge generally facilitates histone-DNA binding, thereby favors chromatin condensation and transcriptional repression (Bannister and Kouzarides 2011 ), but in rare case leads to transcription activation as well (Wang et al. (biomedcentral.com)

Besides, both sugarcane straw retention and soil depths (topsoil and subsoil) significantly altered the co-occurrence patterns and fungal keystone taxa closely related to straw decomposition. (biomedcentral.com)

A potential approach was developed to determine DNA extraction efficiency using fungal reproductive biology and a formula was derived to calculate spore numbers from the quantified DNA amount in the qPCR. (ualberta.ca)

To ensure safety of food products, microbiological tests such as testing for pathogens and spoilage organisms are required. (wikipedia.org)
The RAPD (Random Amplification Polymorphic DNA) allows the amplification of DNA sequences and is a simple and quick technique that does not require prior knowledge on the genomes to characterize organisms, using one randomly determined (usually a decamer) primer 17 . (bvsalud.org)

Given that sex is assumed to come at a cost, these mixed reproductive strategies typical of pathogens have remained puzzling. (elifesciences.org)

The extremely slow growth of this fungus, approximately 1 cm colony in 6 months, and challenges in nucleic acid extractions have hindered research on LLS. (biomedcentral.com)

An evidence-based scientific scrutiny of Irish traditional medicines for their antimicrobial potency is urgently required for combating antibiotic resistant common nosocomial pathogens. (researchgate.net)

The parents and the representative progenies were used in searching for candidate molecular markers using MFLP and NBS-FLP methods. (cropscience.org.au)

Surveillance for respiratory and diarrheal pathogens at the human-pig interface in Sarawak, Malaysia. (cdc.gov)

Specifical clinical tests only focusing on certain pathogens makes it impossible for discovering unknown pathogens as most infectious syndromes present with indistinguishable clinical manifestations. (quickbiology.com)

High throughput sequencing (HTS) has been employed to investigate fungal community composition in soil, however, our understanding of the function and network of fungal community in a fallow straw retention system is very limited [ 24 ]. (biomedcentral.com)
Conclusions This study revealed CRC-associated mycobiome dysbiosis characterised by altered fungal composition and ecology, signifying that the gut mycobiome might play a role in CRC. (bmj.com)

If you do not have Bicoid DNA, it is acceptable to substitute another non-human non-fungal control DNA in this assay along with the appropriate primers and probes. (cdc.gov)

4 Fungal cultures comprised of Penicillium Sp. (eophtha.com)

Although listed here under the category 'Microbial polysaccharides', commercial alginates are currently only produced by extraction from brown seaweeds such as Laminaria hyperborea or L. japonica. (wikipedia.org)
Similarly, like microbial agent's different chemicals are present in order to kill the pathogens. (springeropen.com)

Typically a pre-requisite of sex in pathogens is for at least two different strains to infect the same host. (elifesciences.org)

The distinct methods involve use of nucleic acids and serological assays were implemented to study bacterial and viral characteristics of the infecting pathogen. (springeropen.com)

Anatomy16

Organisms77

Diseases20

Chemicals and Drugs56

Analytical, Diagnostic and Therapeutic Techniques and Equipment32

Phenomena and Processes49

Disciplines and Occupations4

Technology, Industry, Agriculture7

Information Science4

Health Care6

Abstract1

Infection15

Fungi10

Infections13

Microorganisms2

Detection14

Opportunistic fungal1

Isolates were obtained2

Species12

Resistance6

Powdery mildew2

Fusarium4

Pathogenicity3

Spores3

Ribosomal1

Gene8

Samples6

Food spoilage1

Epidemiology2

Inhibition of fungal2

Colletotrichum1

Crops4

Genome1

Inhibit2

Nucleic Acid Extraction1

Sensitivity1

Metabolites1

Microbiota2

Bacteria3

Colonies4

Soil-borne1

Populations3

Diagnosis4

Bacterial 16S1

Diversity5

Interactions1

Diseases4

Identification6

Microbes1

Yeast1

Pseudomonas1

Colorectal cancer1

Genes1

Disease7

Virulence1

Hyphae1

Genetic2

20221

Taxa1

QPCR1

Organisms2

Reproductive1

Fungus1

Antibiotic1

Molecular1

Respiratory1

Infectious1

Composition2

Assay1

Cultures1

Microbial2

Infect1

Viral1