Method tissue than frozen tissue. Medical search. Frequent questions

In a parallel series of electrophysiology experiments in living tissue, the researchers characterized the functional dynamics of the same type of synapses. (sciencedaily.com)
However, as for the effect of the pretreatment method prior to freezing for strawberry tissues, most researchers have focused only on one of the textural parameters, i.e. fracture stress that is considered as a textural index reflecting the cell wall hardness. (ishs.org)
The researchers froze isolated testicle stem cells, while clinics are freezing whole tissue samples. (newscientist.com)
Now, maturing sample preparation methods are delivering more reliable single-nucleus RNA sequencing (snRNA-seq), letting researchers analyze cells in previously intractable tissues. (genomeweb.com)
Many researchers underestimate the length of time needed to develop a sound IHC method. (genengnews.com)
In previous studies, researchers at MGH succeeded in cooling rat livers to minus six degrees by augmenting the protective solution used to cool them with both a modified glucose compound, which lowers the temperature at which the cells freeze, and an ingredient used in anti-freeze, to protect against cold. (cosmosmagazine.com)
The staggered approach, the researchers say, allows the tissue time to adjust and ensures the solution spreads throughout the organ more uniformly. (cosmosmagazine.com)
The researchers have not yet implanted a liver preserved using this new method into a human subject, but they say traditional standards of assessing liver viability indicate that this process will not negatively affect the organ. (cosmosmagazine.com)
Researchers have successfully frozen testicular tissue from monkeys too young to produce sperm and used this tissue to produce a pregnancy resulting in live, healthy offspring. (eurekalert.org)
When the animals approached puberty, the researchers thawed the tissue samples and implanted them, along with a comparison set of fresh, unfrozen samples, back into the animals they came from. (eurekalert.org)
The researchers then isolated sperm from the previously frozen implants and used it to fertilize 138 eggs. (eurekalert.org)
The researchers are attempting to expand their project to include bio-inspired strategies to improve preservation of tissues. (umn.edu)
The researchers have developed a method to advance undeveloped human eggs to near maturity, in laboratory cultures maintained outside the body. (nih.gov)
Finally, the researchers will have to demonstrate that they can freeze and thaw human follicles before growing them in culture. (nih.gov)
Researchers have already identified experimental methods to freeze entire ovaries or strips of ovarian tissue and implant them in a woman's body when she is ready to have children. (nih.gov)

Methods to use frozen testicular tissue in reproduction are still in development. (upmc.com)
The finding is an early step in the development of a method to store testicular tissue for children before they undergo fertility-destroying treatment for cancer or other disorders. (eurekalert.org)
In the current study, the authors removed and froze testicular tissue from five rhesus macaques too young to produce sperm. (eurekalert.org)
The authors note that the study is small, but it helps establish the concept of using immature testicular tissue to preserve fertility. (eurekalert.org)

Explaining the treatment, NIPL Director Jay Mehta said that Cryoablation is a minimally invasive image guided (ultrasound or CT-Scan) treatment that uses extreme cold to freeze and accurately destroy diseased tissue within the tumour zone, quickly and causing bare minimum pain to the patient. (daijiworld.com)
Cryoablation has several advantages over other treatment methods. (daijiworld.com)
The Mehtas say that it is used for treating benign or malignant tumours of the breast, kidney, liver, lung, bone, soft tissues, skin, etc., and the specific application of Cryoablation depends on the condition being treated. (daijiworld.com)
Consultant Radiologist Dr Jankharia in the city said that Cryoablation fills a niche in the overall ablation space and is best for fibromatosis, specific bone and soft tissue tumours, besides liver and lungs. (daijiworld.com)
The Mehtas contend that Cryoablation is a "futuristic and revolutionary technology in India" which the people can take maximum advantage of to freeze and kill the cancer. (daijiworld.com)
Methods used include heating tissue by hot liquids or microwave thermal heating, freezing (CRYOABLATION), chemical ablation, and photoablation with LASERS. (bvsalud.org)
Cryoablation uses tissue freezing (to -70° C) to effect tissue destruction. (msdmanuals.com)

The certified line completely dedicated to the processing of human tissues and cells intended for transplanation. (alchimiasrl.com)
Transcription factor over-expression is a proven method for reprogramming cells to a desired cell type for regenerative medicine and therapeutic discovery. (nature.com)
Normalized area under rank recall curve values for all methods are available in Supplementary Tables 2 - 6 and for epigenomic marks of liver cells in Extended Source Data Fig. 3 . (nature.com)
Rat testicle cells that were frozen for 23 years have produced sperm after being implanted into mice. (newscientist.com)
They took advantage of stem cells from rats that had been isolated and frozen 23 years earlier, thawing and implanting them into the testes of mice. (newscientist.com)
For comparison, the same procedure was also done in other mice using rat cells that had been removed and immediately implanted, as well as with rat cells that had been frozen a few months ago. (newscientist.com)
When the mice's testes were examined, the 23-year-old stem cells had survived and developed into groups of sperm-producing cells, although they made about 20-fold fewer groups of cells than the fresh tissue or recently frozen tissue. (newscientist.com)
The groups of cells from the 23-year-old implants were making mature sperm, but each one made about a third as many as the ones derived from implants of fresh or recently frozen cells. (newscientist.com)
They also took the cells from adult rats, while clinics have to take tissue from children who haven't yet gone through puberty. (newscientist.com)
Most of the samples failed outright, and even in ones that yielded data, there was a lot of background RNA, which he attributed to RNA that had leaked out of the frozen cells and been converted to cDNAs in the 10x droplets. (genomeweb.com)
Single nucleus sequencing really becomes applicable when the user cannot for whatever reason isolate their cells without damaging them," such as with fixed or frozen samples, he added. (genomeweb.com)
But in some cases, especially cryopreserved or post-mortem tissues where some cells die or burst before others, analyzing nuclei leads to a less biased experiment, offering a more complete picture of the cell population at the time of sample collection. (genomeweb.com)
Transmission Electron Micrsocopy (TEM) imaging of cells and tissues generally requires a number of preparation steps. (epfl.ch)
Background/aims To examine the characteristics of infiltrating cells in conjunctival tissues adjacent to the peripheral corneal ulcers of Mooren's ulcer. (bmj.com)
The stained sections were observed and the characteristics of the infiltrating cells in the conjunctival tissues were pathologically examined. (bmj.com)
Results In all patients, infiltration of inflammatory cells was observed in the submucosal connective tissue of the conjunctiva. (bmj.com)
Conclusions In the four Mooren's ulcer cases, infiltrating cells in the submucosa of the conjunctival tissues adjacent to the ulcerative cornea were found to be mainly composed of helper T lymphocytes and macrophages. (bmj.com)
In work with the light microscope, it is difficult to recognize the various components of cells and tissues without differential staining. (columbia.edu)
The research presented in this thesis focuses on developing and applying new tools for interrogating cells and tissues. (kth.se)
We successfully applied our method to cancer cells from metastatic breast cancer patients. (kth.se)
Currently livers are kept at four degrees Celsius to avoid the irreparable damage that can occur when cells freeze, so they only remain viable for around nine hours. (cosmosmagazine.com)
They are continuing these studies and using statistical models to understand the interactions between different solutes and develop rational methods to select the proper combination of molecules to stabilize cells. (umn.edu)
This treatment uses targeted cooling to freeze away fat cells without damaging surrounding skin and tissue. (drumansky.com)
NIPL Managing Director Nainesh Mehta said that as the tissue freezes, ice crystals form, causing cellular damage and destruction… with the extreme cold temperatures freezing and killing the abnormal cells to treat the patient. (daijiworld.com)
This is a good option for some patients, but it is possible that some cancer cells may hitch hike on the ovarian tissue and result in a new cancer after treatment is completed. (nih.gov)
Attempts to store tissues by freezing or drying have focused on adding the protective chemicals some plants and animals have evolved to protect their cells. (newscientist.com)
Fred Levine's team at the University of California, San Diego, was trying to fine-tune a method of drying skin cells using the sugar trehalose, which preserves structures within cells as water is lost. (newscientist.com)
I would have expected to see dramatic damage to cells treated this way," says Mehmet Toner, a tissue engineer at Harvard Medical School in Boston. (newscientist.com)
A unique method of freezing vegetables and fruit that keeps cells alive has been developed, and patented, at Lund University in Sweden. (lu.se)
Using input from two other research areas - how cells can be treated with electrical pulses, and how tissue behaves in a vacuum - the method was developed. (lu.se)
The vegetables are frozen after a few hours, since the cells have been put under stress. (lu.se)
Given this, the ALINE MANICA2 cryopreservation of adipose tissue-derived mesenchymal stem cells (ADMSCs) could be a JULIANA CRISTINA SCHMIDT2 way to collect them in one surgical procedure and after performing fractional fat grafting. (bvsalud.org)
Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)

this array can help scientists high throughput analyze gene and protein expressions in multiple tissue samples, especially analyze different protein levels with antibodies by immunohistochemistry. (wikipedia.org)
Samples sterile porcine cardiovascular tissues, decontaminationantibioticsolutionBASE128_125ml/g 14h 37°C, washing: BASE 6.5 ml/g. 2×5 min. (alchimiasrl.com)
Synaptic structure is typically studied in chemically fixed samples or, as previously done with flash and freeze, with dissociated neurons. (sciencedaily.com)
Adults can have sperm samples frozen before this treatment , but that isn't an option for children who are yet to go through puberty. (newscientist.com)
In such cases, some clinics have been removing and freezing small samples of children's immature testicle tissue in the hope that, if reimplanted when they are adults, it will mature and start making sperm. (newscientist.com)
But for tissues that are hard to dissociate, or for samples with an unknown preservation history, "what you do is hit it with lysis, get the nuclei, and away you go," he said. (genomeweb.com)
The expression of miR-133a in 125 pairs of tissue samples was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) and correlated with patient's clinicopathological data by statistical analysis. (hindawi.com)
Method type(s) for identification: The samples were counted with a liquid scintillation counter LS-750, according to the programme for 14C. (europa.eu)
The levels of organochlorine residues in 40 samples of milk and 20 human adipose tissues from two locations in the northern of Côte d Ivoire were determined. (scialert.net)
This study revealed in general that the level of pesticides in samples of adipose tissue was higher than that in samples of milk from any source (city or rural). (scialert.net)
Adipose tissue samples revealed the presence of polychlorinated biphenyl (PCB), Hexachlorobiphenyl (HCB) in addition to pesticides which are found in the milk Average concentrations of organochlorine pesticides in milk matrix were above the Maximum Residue Limits (MRL) of WHO. (scialert.net)
Human breast milk samples (40 samples) and adipose tissues (20 samples) were collected from mothers (17 to 56 years old, average age 30) living in two different locations in the north of Côte d Ivoire (Korhogo and Sinématiali) from 2004 to 2007. (scialert.net)
Though effective, these methods are not widely available due to issues such as the difficulty in acquiring samples, lack of infrastructure or high cost. (frontiersin.org)
The present collection includes primary material (scabs), frozen liquid cultures and lyophilized samples. (who.int)
The analysis of ADMSCs, after 1 and 3 months of freezing, indicated that samples treated with L-proline + trehalose showed similar viability to those preserved with DMSO and SFB ( p =0.444). (bvsalud.org)
Methods: We analysed methylation status of 807 cancer-related genes in 189 fresh frozen primary breast tumours and four normal breast tissue samples using an array-based methylation assay. (lu.se)

Methods to use frozen ovarian tissue in reproduction are currently experimental. (upmc.com)
Ovarian tissue cryopreservation, though still not widely available, is undertaken in selected cases where time is urgent. (medicalxpress.com)
And it's important that clinicians across the world continue to collect and share data on long-term outcome for all methods, including ovarian tissue preservation, to provide patients with robust information. (medicalxpress.com)
They collected them from the ovarian tissue of 14 cancer patients. (nih.gov)

Collecting and freezing sperm, eggs, or embryos before treatment. (cancer.org)
Developing next-generation methods to treat the most complex cases of infertility (no eggs, no sperm). (upmc.com)
25% goes toward ongoing research to design technologies to produce sperm from the tissue (either inside or outside the body). (upmc.com)
A few months later, they removed the tissue implants and found that both the fresh and frozen tissue had produced sperm. (eurekalert.org)
Men facing such treatments can freeze their sperm for use at a later date. (nih.gov)
Unlike sperm, eggs rarely survive freezing and thawing. (nih.gov)
The best option currently for a female cancer patient to preserve fertility is to collect eggs, fertilize them with sperm, and freeze the resulting embryos. (nih.gov)

By using our newly developed liquid chromatography-tandem mass spectrometry quantification method, we were able to measure the absolute amount of MRP2/Mrp2 in liver tissues and isolated hepatocytes across species. (aspetjournals.org)
Freshly isolated hepatocytes conserved MRP2/Mrp2 protein levels that are comparable with those in the liver tissues. (aspetjournals.org)
Finally, they developed a new method of delivering the preservation solution to the liver. (cosmosmagazine.com)
It also does not spread uniformly, increasing the chance of ice nucleation spreading and freezing the liver. (cosmosmagazine.com)

Pulvini tissue was immediately frozen in liquid nitrogen after harvesting in the greenhouse. (nih.gov)
All the fresh tissues were snap-frozen in liquid nitrogen and stored at −80°C until RNA extraction. (hindawi.com)
It used liquid nitrogen (LN2) for maximum freezing, safety and efficacy. (daijiworld.com)

Dr. Khalife explains that the most appropriate method of fertility preservation is decided on an individual basis. (medicalxpress.com)
Thus, we bridge a gap between traditional tissue preservation methods and novel gene technologytools. (kth.se)

So far, reports Dr. Khalife, the rate of those returning to make use of their frozen eggs and embryos is 16% (61/373), and 44 of them achieved a remarkably high birth rate of 71%, with a twin rate of 9%, when the fertilised eggs and embryos were transferred in an IVF procedure. (medicalxpress.com)
This study showed a patient follow-up rate of 16% to make use of their frozen eggs and embryos, but this rate, says Dr. Khalife, "will definitely increase" in time. (medicalxpress.com)

Peter Jonas, professor at the Institute of Science and Technology Austria (IST Austria), and his group members, first authors Carolina Borges-Merjane (postdoc) and Olena Kim (PhD student), have developed a so-called "flash and freeze" method for studying structure and function of synapses in intact neural circuits in mammalian brain slices. (sciencedaily.com)
The method presented by the Jonas group is a modification of the "flash and freeze" protocol initially used for studying neurons of the worm Caenorhabditis elegans and individually isolated or dissociated mammalian neurons. (sciencedaily.com)
TEM of a thin section of mammalian pancreatic tissue. (genomeweb.com)
Can we apply strategies used by extremophiles to improve mammalian cell and tissue storage? (researchgate.net)
Our experimental focus is on the mammalian setting using multiomics, informatics, mouse genetics, human genetics, single cell studies, and new human tissue platforms. (stanford.edu)

The DNA, RNA, and protein within the tissues are damaged in various levels during the fixation. (wikipedia.org)
Since frozen tissue sections don't go through any fixation procedures, and therefore, the DNA, RNA, and protein in frozen tissues retain their native characteristics much more than in paraffin embedded tissues. (wikipedia.org)
This is done either by chemical fixation or rapid freezing. (epfl.ch)
Following fixation, blocks of tissue must be cut into thin sections. (columbia.edu)
We found a high Pearson correlation of 0.95 between formalin-fixation paraffin embedding (FFPE) and Fresh Frozen (FF) mouse brain datasets. (kth.se)

Women who can't freeze their eggs. (upmc.com)
20% goes toward ongoing research to design technologies to produce eggs from the tissue. (upmc.com)

My research centers around three main questions: How can some animals (extremophiles) survive environmental insults such as complete dehydration, freezing, or lack of oxygen? (researchgate.net)
Initially, Gomez and his colleagues simply wanted to understand how some plants, for example carrots, survive the freezing temperatures of winter. (lu.se)

Indeed, while the average of 2,2-bis(p-chlorophenyl)-1,1,1-trichloroethane (DDT), predominant specie and its isomers in adipose tissue was 10.02 mg kg -1 in countryside and 6.93 mg kg -1 in the city, it was respectively 0.013 and 0.019 mg kg -1 in milk. (scialert.net)
Regarding adipose tissue, this trend was also observed, except for DDT and its metabolites whose values were far below the MRL. (scialert.net)

Cytomics Frozen section procedure MicroArray and Gene Expression (MAGE) Battifora H: The multitumor (sausage) tissue block: novel method for immunohistochemical antibody testing. (wikipedia.org)
The extensive washing procedure, able to remove antibiotic from the solutions and tissues. (alchimiasrl.com)
As expected, the colder the freezing procedure, the less damage the process generally produced. (magritek.com)
Biopsy is often the definitive procedure that provides tissue for microscopic analysis when additional information is required to guide any indicated therapy. (medscape.com)

Advancing stem cell and gonadal tissue transplantation therapies for infertility. (upmc.com)
RESULTS: All 14 cases demonstrated successful tissue transplantation, , with immediate recovery of sensation observed in 10 cases with distal finger pulp defects. (bvsalud.org)

A technique for in situ culture of microorganisms in whole frozen organs. (bmj.com)

Since a favorable dataset for a TCR study is a negative result, it is very important to know that the assay is specific and robust before the test tissues are examined. (genengnews.com)
While biotinylation is a well-established and relatively straightforward technique for labeling biological molecules, FITC may be preferable in a TCR study as it avoids the need for additional avidin-biotin blocking steps in the assay protocol, without which background staining of tissue is likely to prove problematic. (genengnews.com)
In Paper II , we present an assay for spatial RNA quality evaluation, used to estimate the success for tissue specimens before proceeding with more expensive spatial sequencing methods. (kth.se)

Scientists who have been developing antibodies for therapeutic purpose all need their preliminary results from frozen tissues to get approval from FDA. (wikipedia.org)
Essentially, the presence of therapeutic antibody binding in frozen ex vivo tissues is used to give an indication of potential organ toxicity in vivo . (genengnews.com)
The potential for miRNAs as epigenetic biomarkers has long been recognized as a noninvasive method for early CRC detection to enable more effective therapeutic intervention and evaluation of survival [ 8 ]. (hindawi.com)

Some of these studies have used fresh spinal meninges obtained from living animals, [1-4] whereas others have relied on cadaveric human tissue previously frozen for variable periods of time before study. (asahq.org)
And you certainly must NOT leave any previously frozen foods at room temperature for more than two hours. (totalgymdirect.com)
Raw foods that were previously frozen - like fish from a fish market - will be safest if they're cooked first, and then refrozen. (totalgymdirect.com)

Methods Mol Biol. (wikipedia.org)
Methods Cell Biol 50: 209-22). (nih.gov)

In conclusion, miR-133a was downregulated in CRC tissues, but its higher expression correlated with adverse clinical characteristics and poor prognosis. (hindawi.com)

Pulsed electrical field ablation uses a train of high voltage, short duration electrical impulses that kill cardiac myocytes in a non-thermal method. (msdmanuals.com)

3. A freezing profile was defined leading to optimal freezing curve (-1°C/min to -40°C, -3°C/min to -120°C). (alchimiasrl.com)
An optimal freezing profile was set-up. (alchimiasrl.com)
We provide evidence that AME and diffTF are optimal methods for transcription factor recovery that will allow for systematic prioritization of transcription factor candidates to aid in the design of new reprogramming protocols. (nature.com)
Optimal reconstruction method for flat MCP joints is therefore unclear. (bvsalud.org)
Goa, India) and OptiMAL method for the health infrastructure coupled with political detection of pLDH (DiaMed, Cressier sur instability in malaria endemic countries, Morat, Switzerland) [ 10,12 ]. (who.int)

It is unclear if the results will translate to people, as there are some differences between the team's methods and those currently used by fertility clinics, says Rod Mitchell at the University of Edinburgh, UK. (newscientist.com)

In this study, we developed a method to analyze damages on strawberry tissue taking the membrane degradation into account. (ishs.org)
Genetic testing encompasses a broad range of laboratory tests performed to analyze DNA, RNA, chromosomes, proteins, and certain metabolites using biochemical, cytogenetic, or molecular methods or a combination of these methods. (cdc.gov)

You could refreeze foods like poultry or preciously frozen raw meat if it's been handled properly at temperatures below 40 °F - but that's hard to be certain about, so it's better to stay on the safe side. (totalgymdirect.com)

Postmortem bacteriology of the lung by printculture of frozen tissue. (bmj.com)
Lung lobes obtained at necropsy from 100 patients were frozen at -70 degrees C and sectioned as a whole in a sledge microtome as used on complete frozen animals. (bmj.com)
Patients with a suspicious lung mass sometimes receive surgery with no preoperative tissue diagnosis despite-and sometimes in lieu of-modern medical investigations. (nih.gov)
Among patients ultimately found to have lung cancer and who received a lobectomy, performing a frozen section intraoperatively did not increase mean operation time or morbidity. (nih.gov)

Various paraffin tissue arrays are now commercially available from many biotech companies. (wikipedia.org)
Most of the arrays can be easily made by microarraying instrument (Beecher Instruments Inc.). However, paraffin embedded tissues have limitations. (wikipedia.org)

And my luck was that I entered brain research at the time, in the late 1960s, when modern neuroscience was born, and in the labora- tory where a new groundbreaking microscopic technique, the Falck-Hillarp monoamine histofluorescence method, had just been developed. (lu.se)
larvae were identified in the bear paw tissue by microscopic analysis of the material performed by Henry Bishop from DPD's microscopy diagnostic lab. (cdc.gov)

Therefore, many scientific experimental results from formalin fixed tissues are not reliable. (wikipedia.org)

Alchimia is one of the leading European manufacturers of medical devices for ophthalmic surgery and human tissue banking with the aim of improving the daily routine of the medical personnel and the quality of life of patients. (alchimiasrl.com)
The expression of miR-455 was lower in HCC tissues and cell lines than in nontumor tissues and normal cell line, and downregulation of miR-455 was connected with worse outcome of HCC patients. (hindawi.com)
Methods This study involved four eyes of four patients with Mooren's ulcer and who were considered to be in need of surgical treatment. (bmj.com)
The patients' resected conjunctival tissues were embedded and frozen. (bmj.com)
Among those patients with no preoperative tissue diagnosis, 97 (47.1%) proceeded to surgery without attempts at preoperative diagnosis, and 109 (52.9%), after attempts at preoperative diagnosis failed to yield a positive diagnosis. (nih.gov)
It requires just a small incision or a needle puncture, results in less trauma to the patient and enables faster recovery compared with open surgery, can be performed under local anaesthesia in most cases, and since it is precise and targeted at the abnormal tissue while preserving the surrounding healthy tissues, it eliminates hospital stay for most patients," elaborated Nainesh Mehta. (daijiworld.com)
METHODS: Patients undergoing open surgical repair for a complete thumb UCL rupture were included between December 2011 and February 2021. (bvsalud.org)
METHODS: From January 2016 to January 2020, a total of 14 patients , 9 males and 5 females, ages ranging from 22 to 69 years old, and with volar soft tissue defects in the middle and distal digits 2 to 4, underwent surgical reconstruction using the V-Y shaped flap with digital artery and nerve at the metacarpophalangeal joint. (bvsalud.org)

To address this and to also study the oncogenic potential of unregulated function of dominant regulators of epithelial homeostasis noted above, we developed Multi-Functional Human Tissue Genetics noted above which, when combined with skin tissue regeneration on immune deficient mice, has permitted the molecular reconstruction of events sufficient to trigger human cancer. (stanford.edu)
Since that time, advances in scientific research and technology have led to a substantial increase both in the health conditions for which genetic defects or variations can be detected with molecular methods and in the spectrum of the molecular testing methods ( 1 ). (cdc.gov)
Use of EIAs, tissue culture, molecular probes, and the polymerase chain reaction has improved the diagnosis of diarrhea caused by bacteria, and special concentrating and staining techniques have improved the process of detecting parasites such as Cryptosporidium and I. belli. (cdc.gov)

Tests commonly employed in frozen tissue array include immunohistochemistry, and in situ hybridization. (wikipedia.org)

With our method, we can now use the same type of preparation used to study synaptic function to simultaneously study structure," Olena Kim points out. (sciencedaily.com)
One such preclinical safety study is the assessment of tissue cross-reactivity (TCR). (genengnews.com)
However, these methods add to the overall study cost, and sufficient soluble antigen is not always available. (genengnews.com)
In order to study tissues with a microscope they must be preserved (fixed) and cut into sections thin enough to be translucent. (columbia.edu)

Schoenberg Fejzo M, Slamon DJ: Frozen tumor tissue microarray technology for analysis of tumor RNA, DNA, and proteins. (wikipedia.org)
Strikingly, we also observed similar iCNV profiles in both tumor and benign tissue areas from prostate and skin cancer. (kth.se)
Higher miR-133a expression in tumor tissues was associated with development of distant metastasis, advanced Dukes and TNM staging, and poor survival. (hindawi.com)
MiR-133a has been reported on several occasions to be downregulated in cancers when compared to normal adjacent tissue, and it has been implemented as a tumor suppressor targeting several oncogenes. (hindawi.com)

Removal of tissue by vaporization, abrasion, or destruction. (bvsalud.org)

Tissue microarrays from frozen tissues-OCT technique. (wikipedia.org)
Further, we investigated the effect of the pretreatment with the CaCl 2 solution, which is well known as a texture preserving technique for freeze-thawing fresh strawberry. (ishs.org)
The new technique allows them to be "supercooled" without ice storage to minus four degrees, maintaining tissue for up to 27 hours. (cosmosmagazine.com)
Magnetic resonance imaging (MRI) was tested as a technique to map and quantify tissue damage from freezing in fish. (magritek.com)

3. Freezing method: several profiles were tested on aortic, pulmonary and pericardium specimens. (alchimiasrl.com)
In Paper III , we present a protocol for performing spatial mRNA genome-wide expression profiling of FFPE tissue specimens. (kth.se)

We compare methods that use gene expression, biological networks and chromatin accessibility data, and comprehensively test parameter and preprocessing of input data to optimize performance. (nature.com)
The findings suggest that children who have testicle tissue frozen before cancer treatment may be able to have the tissue reimplanted so they can one day have their own biological children through in vitro fertilisation (IVF), says Eoin Whelan at the University of Pennsylvania in Philadelphia. (newscientist.com)
The new approach uses machine perfusion - a way of delivering oxygen and nutrients to capillaries in biological tissues while outside the body - at four degrees with the traditional protective solution, then slowly lowers the temperature while increasing the concentration of the new protective additives. (cosmosmagazine.com)
They are characterized by their bioaccumulation properties in the fatty parts of biological beings, such as blood, breast milk and fatty tissues via the food chain ( Smith and Gangolli, 2002 ). (scialert.net)

The tissue sections were then subjected to H&E and immunohistochemical staining. (bmj.com)

When you don't have enough time to thaw frozen foods, just remember: it's safe to cook foods from the frozen state. (totalgymdirect.com)

RAB18 was upregulated in HCC tissues and cell lines, and the expression of RAB18 was regulated by miR-455. (hindawi.com)
Endogenous expression levels of several potential target genes were determined by qRT-PCR and correlated using Pearson's method. (hindawi.com)
Methods: We analyzed the expression of toll-like receptors (TLRs) and adenosine receptors during traumatic skeletal muscle injury. (cdc.gov)
mRNA expression and immunostaining of these receptors were evaluated in mouse skeletal muscle injured by freezing. (cdc.gov)

RESEP-untreated tissues: 11/12 strains on agar plates (at less extent for 4 strains), 10/12 strains on BactAlert. (alchimiasrl.com)
five primary scab isolates, four of nine frozen cultures and all six lyophilized strains have demonstrable viability. (who.int)

Sections from this block are cut using a cryostat, mounted on a microscope slide and then analyzed by any method of standard histological analysis. (wikipedia.org)

The association of L-proline and trehalose kept ADMSC viable for 30 and 90 days of freezing, and could be an alternative as a cellular cryopreservative without toxicity and enabling the use of serial fat grafting. (bvsalud.org)

Water containing the trehalose is then injected and replaces the air in the tissue. (lu.se)
The cellular fraction was processed and frozen with L-proline (1.5M) + trehalose (0.2M) or with DMSO + fetal bovine serum (FBS) as control. (bvsalud.org)

Results indicate a wide variety of bacteria associated with healthy colonies and an even higher diversity associated with those corals presenting tissue necrosis, which displayed nearly 25% more microorganisms. (nature.com)

The U.S. Department of Agriculture's guidelines on food safety recommends three methods for thawing your food: microwaving, gradually thawing your food in a microwave, or soaking food in a cold water bath. (totalgymdirect.com)

Malignancy is suspected when persistent oral mucosal lesions are red or red and white or when they are ulcerated, indurated, or fixed to deeper tissues. (medscape.com)

These new models, which we term Multi-Functional Human Tissue Genetics, allow up to 10 alleles or more to be altered simultaneously, permitting genetic experiments with an unprecedented degree of rapidity and complexity. (stanford.edu)

CoolSculpting has made a name for itself as one of the most effective methods of nonsurgical body contouring today. (drumansky.com)
Physical therapy is the primary treatment for frozen shoulder, but surgery may be suggested if nonsurgical methods are not effective. (healthline.com)

The function of neurons is usually studied in slices of brain tissue, in which networks remain intact. (sciencedaily.com)

TCR studies are also used to compare staining patterns between human and animal tissue, providing additional justification for the choice of models used to generate other preclinical safety data. (genengnews.com)
Unlabeled human or humanized antibodies can be detected by precomplexing with an anti-human antibody before applying to the test tissues. (genengnews.com)
1 The Control Item, essentially an isotype control, is usually a molecule identical in structure to the test item (including labels such as biotin or FITC) but raised against a molecule unlikely to be found in human tissue-for example, green fluorescent protein, a plant protein, or even snake venom. (genengnews.com)
A machine profusion process helps supercool human livers without freezing the tissue. (cosmosmagazine.com)
Twenty-four fresh-frozen, human thumbs were tested for flexion, extension and valgus stability of the MCP joint. (bvsalud.org)

Do not wish to delay or interrupt their gender-affirming treatments to avoid female puberty and egg production to be retrieved and frozen. (upmc.com)

still, diffusion cell studies using fresh and frozen meningeal tissues continue to be submitted to anesthesia journals for publication (personal observation, C.M.B.). To address this concern, we directly compared the permeability of morphine through fresh and dry-frozen spinal meninges. (asahq.org)
Additionally, the diffusion of water molecules in tissue was measured by using NMR to evaluate the degradation of cell membrane. (ishs.org)

OBJECTIVE: To explore a surgical method for the reconstruction of volar soft tissue defect and sensory and vascular repair in middle and far phalangeal digits. (bvsalud.org)

Conclusions: Both receptor types may be potential targets for stimulation of skeletal muscle tissue regeneration and functional restoration after injury. (cdc.gov)

We measured the permeability of morphine through fresh spinal meninges obtained from anesthetized monkeys and then repeated this measurement through the same tissues after they were dry-frozen at -20 [degree sign]C. (asahq.org)
[1-4] Briefly, thoracic spinal cords of seven Macaca nemestrina monkeys were carefully removed en bloc and placed in PBS within 10-30 min of the animal's death by barbiturate overdose. (asahq.org)

Frozen tissue array consists of fresh frozen tissues in which up to 50 separate tissue cores are assembled in array fashion to allow simultaneous histological analysis. (wikipedia.org)
Consequently, frozen tissue array should be the best tool for high throughput analysis on this purpose. (wikipedia.org)
Methods for isolation and analysis of polyribosomes. (nih.gov)
This set-up allows neuroscientists to stimulate neurons and freeze the tissue immediately afterwards for analysis by electron microscopy, so that changes in anatomy right after stimulation become visible. (sciencedaily.com)
Focusing on computational and statistical aspects of MR discovery, the ARACNe-MRA (Algorithm for the Reconstruction of Accurate Cellular Networks-Master Regulator Analysis) method has shown competent performance in this regard [ 4 ]. (aging-us.com)
To provide repeatable, objective classification, image analysis using a convolutional neural network was then performed on the MRI data to identify regions of damaged tissue. (magritek.com)

As a result, it was revealed that both cell wall and cell membrane got damaged, so that the remarkable tissue softening was induced. (ishs.org)
However, for strawberry tissue, damage on cell membrane was found to be dominant in textural softening. (ishs.org)
It was also clarified that the pretreatment with the CaCl 2 solution would suppress the decrease of fracture stress reflecting cell wall hardness but could not improve initial modulus reflecting cell membrane tension after freeze-thawing. (ishs.org)
As a conclusion, it was found to be necessary to control the damage on cell membrane to prevent the softening of strawberry tissue after freeze-thawing. (ishs.org)

Skin malignancies, including epidermal squamous cell carcinoma (SCC), alone account for nearly as many cancers as all other tissues combined. (stanford.edu)

Here, we take a synapse, stimulate it with light and, within milliseconds, shoot it into a chamber that freezes the structure at minus 196 degrees Celsius and at a pressure of 2,000 bar. (sciencedaily.com)

The dermis offers low resistance, as do almost all internal tissues except bone, which is a poor conductor of electricity. (medscape.com)

Whether to take a core biopsy or wedge excision biopsy for frozen section assessment intraoperatively was decided at the surgeon's discretion. (nih.gov)
Oral tissue biopsy may be necessary for lesions that cannot be diagnosed on the basis of the history and clinical findings alone. (medscape.com)

Fundamentally it consists of a chemical or physical method of killing the tissue and yet retaining characteristic peculiarities of shape and structure. (columbia.edu)

Multicellular systems (tissues, aggregates) typically have a poor response to freezing. (umn.edu)

Anatomy3

Organisms1

Diseases4

Chemicals and Drugs13

Analytical, Diagnostic and Therapeutic Techniques and Equipment24

Phenomena and Processes5

Disciplines and Occupations2

Information Science2

Health Care3

Researchers15

Testicular tissue4

Cryoablation7

Cells33

Samples16

Ovarian tissue4

Sperm7

Liver4

Liquid nitrogen3

Preservation2

Embryos2

Mammalian5

Fixation5

Eggs2

Survive2

Adipose2

Procedure4

Transplantation2

Organs1

Assay3

Therapeutic3

Previously frozen3

Biol2

Characteristics1

Ablation1

Optimal5

Fertility1

Analyze2

Temperatures1

Lung4

Paraffin2

Microscopic2

Experimental1

Patients8

Molecular3

Situ1

Study4

Tumor4

Destruction1

Technique4

Specimens2

Biological4

Immunohistochemical1

Thaw1

Expression4

Strains2

Microscope1

Viable1

Trehalose2

Microorganisms1

Microwave1

Deeper1

Genetic1

Nonsurgical2

Brain tissue1

Human5

Puberty1

Diffusion2

Soft tissue1

Regeneration1

Monkeys2

Analysis6

Cell membrane4

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Degrees1

Bone1

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Consists1

Typically1