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Comparison of Rapid Enzyme-linked Immunosorbent Assay and Immunomagnetic Separation Methods for Detection of Escherichia coli O157 in Fecal, Hide, Carcass, and Ground Beef Samples. (ttu.edu)
Dive into the research topics of 'Comparison of Rapid Enzyme-linked Immunosorbent Assay and Immunomagnetic Separation Methods for Detection of Escherichia coli O157 in Fecal, Hide, Carcass, and Ground Beef Samples. (ttu.edu)
This is partly due to the lack of a readily available, reliable immunomagnetic separation technique (IMS). (usda.gov)
Here we investigated the use of lectin-magnetic separation (LMS) for isolating Toxoplasma gondii oocysts from water sample concentrates, with subsequent detection by microscopy or molecular methods. (usda.gov)
International Journal of Pharmaceutics Volume 215 Issue 1-2 Pages 45-50 (2001) Chromatography: Separation and Indirect Detection of Amino-acids by Reversed Phase ion-pair Chromatography. (wikipedia.org)
We report a method that combines Immunomagnetic separation and polymerase chain reaction (IMS-PCR) to detect Salmonella sp. (edu.au)
Immunomagnetic separation (IMS) beads were used to assist the accumulation of bacteria into the path of the beam of the excitation laser. (rsc.org)
Pathogen testing methods, including polymerase chain reaction and immunomagnetic separation, which increase the sensitivity and speed of pathogen detection, as well as recent advancements in nano-biotechnology, present attractive prospects for market participants throughout the projection period. (credenceresearch.com)
The NanoSPoD joint project aims to establish a nanoprobe-based magnetic separation method (NBMS) for plant pathogens, to develop new molecular biological detection methods and to use and evaluate NBMS together with new and already established diagnostic methods for the detection of QPs and RNQPs. (computomics.com)
In order to accelerate Salmonella diagnosis, we used the immunomagnetic separation assay associated with bacteriophage P22 for the rapid detection of the following Salmonella serovars in chicken rinses of drumsticks, artificially contaminated with 5, 10, and 100 CFU/25mL of bacteria: Salmonella enterica subsp. (pvb.com.br)
The isolation of STEC from samples positive for the presence of the genes specifying the serogroups in the scope of this method can be facilitated by using serogroup-specific enrichment techniques (e.g. immunomagnetic separation, IMS). (iso.org)
Three methods namely: Rand Water Method 06 (2007), US-EPA Method 1623 (2005) and Drinking Water Inspectorate standard operating procedures (2003) were compared and key different steps in the methods were identified (wrist action speed, centrifuge speed, immunomagnetic separation procedures and addition of pre-treatment steps). (vut.ac.za)
Different experiments were conducted to verify and evaluate the difference between two wrist action shaker speeds, three different centrifuge speeds, two slightly different immunomagnetic separation procedures and when a pre-treatment step was included in the method. (vut.ac.za)
CD138 + cells from a single patient with MM were isolated with informed consent from bone marrow (BM) mononuclear cells using positive immunomagnetic column separation (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany). (spandidos-publications.com)

This method is based on the immunomagnetic enrichment of CTCs from patient's blood samples, with subsequent staining of these cells. (iiarjournals.org)
By technology, the two major segments of the global market for CTCs prognostic technologies include tumor cell detection and tumor cell enrichment. (dailybn.com)
On the other hand, the tumor cell enrichment segment is divided into immunological and immunomagnetic methods, filtration, and centrifugation. (dailybn.com)
In these sub-segments, immunomagnetic methods accounted for the largest share in the overall tumor cell enrichment segment. (dailybn.com)
CTCs are present in the bloodstream at a very low concentration, thus their detection and characterization require highly sensitive and specific methods, which consist of a combination of enrichment (isolation) and detection (identification) strategies ( Table 1 ) and both steps are essential components of the identification process ( 19 ). (amegroups.org)
Previously defined strategies for CTC detection, isolation, and enrichment are based on procedures including density, immunomagnetic, size-exclusion and/or circulation manipulation in microfluidic devices (4-8). (biotechnologyconsultinggroup.com)
The results confirm the utility of the qPCR method without the pre-enrichment step in tetrathionate as a rapid test for Salmonella spp. (pvb.com.br)
CTCs were captured onto a very small area (1 mm2) with a high enrichment ratio.27 Although eDAR was more sensitive than the CellSearch method in detecting CTCs from metastatic breast cancer patients 27 the original version of eDAR still has several factors that may limit its clinical application. (conferencedequebec.org)
The aim of this thesis was to realize single cell isolation by means of automated immunomagnetic bead enrichment and antibody-based fluorescence detection in a chip-based microfluidic system and the molecular biological characterization of these cells to assay tumor features from liquid biopsy. (fraunhofer.de)
Therefore, this work compares manual and automated immunomagnetic enrichment in terms of cell recovery in dependency of cell line and input number, bead characteristics, biofunctionaliza-tion protocol and blood cell contamination focusing on head and neck squamous cell carcinoma (HNSCC) and metastatic breast cancer. (fraunhofer.de)
In the microfluidic benchtop platform CTCelect, we characterized each process step with two different carcinoma cell lines (MCF-7, SCL-1) demon-strating up to 87 Percent enrichment, 73 percent optical detection and dispensing efficiency. (fraunhofer.de)

Detection sensitivity for both RT-LAMP assays was greater by a factor of 100 compared to the conventional reverse transcription polymerase chain reaction assays. (ppjonline.org)
After isolation, the source and genetic make-up of CTCs are characterized using nucleic acid-based methods, such as quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR), or cytometric-based methods, such as flow cytometry and enzyme-linked immunospot assay technology ( 4 ). (iiarjournals.org)

We created a smartphonebased, electrochemical, immunomagnetic assay for the detection of ochratoxin A in real samples, providing a solid basis forsensing of further analytes. (kobv.de)
Based on these findings, we created a smartphone-based, electrochemical, immunomagnetic assay for the detection of ochratoxin A (OTA) and ergometrine in food samples. (kobv.de)

Epithelial immunospot (EPISPOT) is an antibody-based method for quantification of live CTCs by detection of CTCs which are capable of secreting proteins such as cathepsin D, MUC1 and CK19. (medscape.com)
CTCs in the blood sample are enriched by negative selection using anti-CD45 immunomagnetic beads. (medscape.com)
The large heterogeneity of melanoma CTCs has hindered their detection and clinical application. (nature.com)
To explore the heterogeneity of CTCs, a number of antibody-based and transcript-based assays have been developed for detection of a broader range of melanoma CTCs. (nature.com)
We present a method for the detection of CTCs by real-time PCR on different cytokeratin markers. (iiarjournals.org)
Numerous studies suggest that detection of CTCs in the blood or disseminated tumour cells (DTCs) in the bone marrow of patients with cancer is linked to poor outcomes of the disease and reduced treatment efficiency ( 5 , 6 ). (iiarjournals.org)
There are several different methods for the detection of DTCs and CTCs from bone marrow or blood ( 7 - 9 ). (iiarjournals.org)
In this study, we present a novel nucleic acid-based method for the detection and possible quantification of CTCs utilizing quantitative TaqMan real time PCR. (iiarjournals.org)
The enumeration of circulating tumor cells (CTCs) represents an effective prognostic and predictive biomarker, which is able to monitor efficacy of adjuvant therapies, detect early development of (micro)metastases and at last, assess therapeutic responses of advanced disease earlier than traditional imaging methods. (amegroups.org)
In the last decade, the detection of disseminated tumor cells (DTCs) in the bone marrow and circulating tumor cells (CTCs) in the blood have demonstrated to provide useful information for the clinical management of BC by predicting treatment benefit earlier than traditional imaging methods. (amegroups.org)
The aim of this review is to describe the current state of the art on CTCs detection and clinical use, the evidence to demonstrate their clinical validity, and their potential impact for both future clinical trial design and, decision-making process in our daily practice. (amegroups.org)
Herein, we review the history and the current status of CTC detection, discuss the prognostic, predictive, and diagnostic capabilities of these technologies, and describe application of CTCs as models for the study of tumor biology. (amegroups.org)
However, comparable to CellSearch, most of these methods are limited by the heterogeneous nature of CTCs, often missing the EpCAM? (biotechnologyconsultinggroup.com)
Ensemble-decision aliquot position (eDAR) is a sensitive and high-throughput method to analyze circulating tumor cells (CTCs) from peripheral blood. (conferencedequebec.org)
Nonetheless the small number of CTCs compared to billions of blood cells in a patient sample requires sensitive methods to obtain reproducible tumor cell recovery rates. (fraunhofer.de)
in this scenario, careful consideration must be given to the isolation approach, whereas methods that discriminate against certain subpopulations may result in the exclusion of CTCs that carry biological relevance. (oaepublish.com)
An emerging method to detect metastasis is the analysis of circulating tumor cells (CTCs), which has shown promise in filling the gaps left by other diagnostic methods. (iiarjournals.org)

These findings indicate that LMS with WGA coupled to magnetic beads could be an efficient isolation step in the analysis of water sample concentrates for T. gondii oocysts, with detection either by microscopy or by qPCR. (usda.gov)
Rand Water has adopted their method for isolation and detection of Cryptosporidium oocysts and Giardia cysts in water from United State Environmental Protection Agency (US-EPA) Method 1623, 1999. (vut.ac.za)
A study was done to improve the performance of the Rand Water Method 06 (2007) used for isolation and detection of Cryptosporidium oocysts and Giardia cysts. (vut.ac.za)
Total exosomes enriched from cell culture (by using the Total Exosome Isolation reagents or ultracentrifugation) can be further purified into specific subpopulations by immunomagnetic capture. (thermofisher.com)
Immunological techniques, which are the most widely used methods, employ immunomagnetic isolation ( 4 ). (iiarjournals.org)

The developed in-situ ﬂuorescent immunomagnetic sensor approach offers distinct advantages because it is rapid, highly sensitive, and easy to use and could therefore be potentially used as a pathogen screening tool. (docksci.com)
comprehensive pathogen detection methods will enable implementation of effective strategies to avoid, or when needed intervene in, the distribution of foods that have been contaminated with foodborne pathogens. (docksci.com)
The complexity of food matrices, the wide variety of microorganisms therein, and the varying growth and replication traits of target pathogenic microorganisms that, when present, are often in much smaller numbers than the nonpathogenic microorganisms, pose signiﬁcant challenges for pathogen detection. (docksci.com)
Associations between faecal pathogen detection, E. coli concentrations and youth exhibitor biosecurity practices at California county fairs. (ucdavis.edu)
As new and promising detection approaches, an immunomagnetic analysis technique as well as an innovative pathogen screening method based on Next Generation Sequencing (NGS) will be investigated. (computomics.com)
Salmonella detection is a key point in food safety testing, because of the frequent association of this pathogen with food poisoning in humans. (pvb.com.br)

A simple ﬂuorometric immunological method in combination with a magnetic concentration step was developed for rapid detection of target bacteria with high sensitivity and speciﬁcity in less than 2 h without enumeration. (docksci.com)
The purpose of this review is to provide a complete update of the various analytical methods for virus detection, such as molecular, immunological, sensor-based and other detection assays, along with the advantages and limitations of these strategies. (frontiersin.org)

The results are in good correlation with the established photometric detection method, providing a solid basis for sensing of further analytes in HRP-based assays using the newly developed miniaturized smartphone-based, electrochemical, immunomagnetic assay. (kobv.de)
Immunomagnetic HIV-1 capture analysis was used to determine the cellular origin of cell-free virus particles present in all 30 plasma samples and indices of immune activation were measured using enzyme-linked immunosorbent assays. (lww.com)
The detection of tau in plasma has been limited due to its low abundance until recent technical development of ultrasensitive assays. (biomedcentral.com)

This study reports the development of a sensitive magnetic bead-based enzyme-linked immunoassay (MELISA) for the pan-reactive detection of the Influenza A virus. (flutrackers.com)
Immunoassay refers to a class of analytical methods based on antigen-antibody-specific interactions. (peertechzpublications.com)
There are two formats in each immunoassay test: 1) sandwich format (for macromolecule detection, such as foodborne pathogens and allergen proteins). (peertechzpublications.com)

A biotin-nanoparticle and immunomagnetic bead-based assay was developed. (flutrackers.com)
We evaluated the feasibility of an early prototype ligand-coated magnetic bead technology to concentrate M. tuberculosis prior to detection by LED-based fluorescence microscopy compared with direct Ziehl-Neelsen microscopy and direct and concentrated fluorescence microscopy in a reference laboratory in Kampala, Uganda. (biomedcentral.com)
Both magnetic bead and concentration by centrifugation led to reduced specificity compared with the direct smear methods. (biomedcentral.com)
A recent study reported encouraging performance of ligand-coated magnetic beads in combination with FM, in detection of M. tuberculosis in a panel of frozen sputum samples, with good correlation reported between magnetic bead concentration and centrifugation [ 7 ]. (biomedcentral.com)

However, these conventional methods are time-consuming, laborious, and/or lack in sensitivity in part due to the requirement of cultivation steps. (docksci.com)
However the main limitation of the method is its low sensitivity in programmatic settings, particularly in HIV co-infected patients [ 2 ]. (biomedcentral.com)
Various physical and chemical sputum processing methods have been identified which can improve the sensitivity of microscopy. (biomedcentral.com)
Fluorescence microscopy (FM) gives an average increase of 10% sensitivity over the ZN method, while retaining specificity [ 4 ], a finding which has had little relevance in disease endemic countries until the recent development of inexpensive fluorescence microscopes illuminated by light emitting diodes (LEDs). (biomedcentral.com)
The use of this novel roll-to-roll fabricated SERS substrate was combined with optimised gold nanoparticles to increase the detection sensitivity. (rsc.org)
The sensitivity of our SERS detection was improved through optimization of the concentration of the sample inside the hydrophobic polydimethylsiloxane (PDMS) wells. (rsc.org)
The aim of the project is to universally improve the sample quality for all downstream molecular biological analysis methods by employing NBMS, so that a higher sensitivity and robustness of these methods is attained. (computomics.com)

The assay combines immunomagnetic beads and biotin-nanoparticle-based detection to quantify a highly conserved viral nucleoprotein in virus lysates. (flutrackers.com)

In-situ fluorescent immunomagnetic multiplex detection of foodborne pathogens in very low numbers. (docksci.com)
Furthermore, the method was highly speciﬁc in detecting the target pathogens in a multiplex format. (docksci.com)
This regulation requires enhanced detection of pathogens in both imported and domestically produced foods coupled with effective intervention methods to achieve food safety. (docksci.com)
The rapid and accurate detection of food pathogens plays a critical role in the early prevention of foodborne epidemics. (rsc.org)
Detection methods will be established for different user groups, which even allow the identification of previously unknown pathogens. (computomics.com)

CTC were detected by multiplex immunostaining and multi-transcript detection. (nature.com)
The IMS-PCR assay exploits selective extraction of bacteria using antibody coated superparamagnetic particles and multiplex PCR amplification that enable detection of Salmonella sp. (edu.au)

Immunomagnetic capture is used in a number of platforms as an alternative to filtration or centrifugation to concentrate bacteria in clinical specimens. (biomedcentral.com)

The colorimetric detection signal was amplified using biotinylated silica nanoparticles (NP). (flutrackers.com)
These nanoparticles were functionalized on the surface with short DNA spacers bearing biotin groups by an automated supported synthesis method performed on nano-on-micro assemblies with a DNA/RNA synthesizer. (flutrackers.com)

Thus we demonstrate IMS-PCR with improved surface chemistry and covalent attachment of antibody to be rapid, specific and cost effective detection method for Salmonella species in clinical isolates. (edu.au)

The application of molecular techniques in food is large, several authors comment on the detection and quantification of strains of pathogenic microorganisms present in foods and the presence of toxins and waste allergens not related to specific goods in production lines. (peertechzpublications.com)
Here we identified 66 eligible studies reporting quantification of plasma tau and phosphorylated tau 181 (ptau181) using four ultrasensitive methods. (biomedcentral.com)

and Giardia duodenalis are available and widely used, equivalent methods for analyzing water samples for Toxoplasma oocysts are lacking. (usda.gov)
Methods for detecting the presence of Cryptosporidium oocysts and Giardia cysts have been developed and continuous improvement is being done to improve the recovery rate of the target protozoa. (vut.ac.za)
The optimization of the Rand Water Method 06 (2007) was successful as the recovery rate of Cryptosporidium oocysts and Giardia cysts from water increased. (vut.ac.za)

Objective:To investigate the clinical characteristics and prognostic factors of 2019 novel coronavirus (2019-nCoV) Omicron variant infected cases.Methods:A total of 987 coronavirus disease 2019 (COVID-19) adult imported cases admitted to Shanghai Public Health Clinical Center, Fudan University from July 1, 2021 to January 6, 2022 were recruited. (bvsalud.org)

We examine the efficiency to capture and detect target bacteria in suspension using immunomagnetic particles in two different attachment modes namely, random assembly and oriented assembly of antibodies. (edu.au)

Different SERS substrates have been studied for the detection of foodborne microbes. (rsc.org)

This method uses either epithelial cell-specific markers which are generally expressed in all tumor cells, or tumor markers expressed by specific types of cancer ( 13 ). (iiarjournals.org)

Although standard methods for analyzing water samples for the protozoan parasites Cryptosporidium spp. (usda.gov)

This review describes the current state of the art on CTC detection strategies, the evidence to demonstrate their clinical validity, and their potential impact for both future clinical trial design and, decision-making process in our daily practice. (amegroups.org)
Objective:To evaluate the efficacy and safety of endoscopic submucosal dissection (ESD) for early hypopharyngeal carcinoma and precancerous lesions.Methods:Clinical data of 41 patients who received ESD for early hypopharyngeal carcinoma and precancerous lesions from August 2013 to August 2019 in the Department of Endoscopy of Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College were retrospectively analyzed. (bvsalud.org)
Considering the calamity of ZIKV infection, there is an urgent need to develop rapid detection methods for ZIKV along with DENV, which shares common clinical symptoms with ZIKV. (frontiersin.org)
Currently, detection of breast cancer metastasis relies on clinical manifestations of the spread to distant organs, biopsies of affected organs, radiological evaluations, imaging methods and serum tumor markers ( 3 , 4 ). (iiarjournals.org)
Although mammographic screening has reduced the mortality rate associated with metastasis as a result of early diagnosis ( 2 ), the methods described above are frequently inept at detecting metastasis at the earliest stage and at accurately predicting the clinical outcome of the disease ( 3 , 4 ). (iiarjournals.org)

This method is rapid and inexpensive and highly specific for Mycobacterium tuberculosis in such settings. (biomedcentral.com)
Hence, the development of innovative, rapid and robust diagnostic methods is required, which are of major importance for plant protection and serve to identify, monitor and develop appropriate management and control strategies. (computomics.com)
Consequently, there is a need to develop a rapid and efficient detection method to accurately identify LNYV and CMV infections and help limit their spread. (ppjonline.org)
This review summarizes the currently available methods employed for the rapid detection of ZIKV and suggests an innovative approach involving the application of a liposome-based hypothesis for the development of new strategies for ZIKV detection and their use as effective biomedicinal tools. (frontiersin.org)

Although these biosensors are currently among the less popular tools for human disease detection, they have become useful tools for the screening and detection of pathogenic bacteria, fungi, and viruses because of their versatile advantageous features compared to other sensing devices. (frontiersin.org)

The food industry often runs own lab procedures, for example, for mycotoxin analysis, but it is a major goal to simplify analysis, linking analytical methods with smart technologies. (kobv.de)
The integration of various analytical methods on ancient silk fabrics enables us to learn various information about their textile forms, silk animal origins, colors and dyestuffs, revealing the highly developed silk waving techniques and prosperous costume culture of the Liao Dynasty. (springeropen.com)
Identifying and examining ancient silks are crucial in both archaeology and academia, thus many analytical methods have currently been employed in this field. (springeropen.com)

The hypothesis that disagreements within the literature regarding bacterial spectra results from conditions present during the detection process has not been supported. (rsc.org)

This can be achieved either by cytometric techniques or by nucleic acid-based methods ( 3 ). (iiarjournals.org)

The study of ancient silk dyeing processes has also gained significant attention, with LC-MS/MS being the most widely utilized detection technique. (springeropen.com)

METHODS: A total of 167 patients from the GeparX trial were analyzed for DTCs at baseline by immunocytochemistry using the pan-cytokeratin antibody A45-B/B3. (bvsalud.org)

In general, the most known and used methods are Western Blots (WB), Enzyme-Linked Immune Sorbent Assay (ELISA), Lateral-Flow Assay (LFA), and Dot-Blot Assay (DBA) and have been applied in the detection of carcinogenic compounds, pathogenic and allergenic microorganisms using antibodies [8]. (peertechzpublications.com)

The majority of the approaches are either based on costly patterning techniques on silicon or glass wafers or on methods which have not been tested in large scale fabrication. (rsc.org)

However, many such methods require expensive centrifuges or reagents which would add substantially to the cost and complexity of performing smear microscopy. (biomedcentral.com)
Instrumental analyses, commonly used in physical-chemical methods, present systematic errors related to the equipment and reagents used, as well as errors in reading, and weighing by the operator, and require several hours before the results are obtained. (peertechzpublications.com)

Without limitation, some embodiments comprise methods, systems, and/or compositions relating to microRNAs and/or cell based therapies and the use of the same in the research, diagnosis, or treatment of injury or disease. (justia.com)

At moderately acidic conditions (pH 4), neither with carbon nor with gold electrodes a reproducible electrochemical detection of TMB could be achieved. (kobv.de)

Molecular detection of Sarcocystis neurona in cerebrospinal fluid from 210 horses with suspected neurologic disease. (ucdavis.edu)

Additionally, imaging methods such as bone scintigraphy, liver echography and chest X-ray are to be performed bianually. (iiarjournals.org)

Simpler concentration methods that could be applied in peripheral laboratories are urgently needed. (biomedcentral.com)

CTC detection using our multimarker approach was associated with shorter overall and progression-free survival. (nature.com)
To provide a straightforward approach for the miniaturization of the detectionstep, we have studied the pitfalls of the electrochemical TMB detection. (kobv.de)
To provide a straight-forward approach for the miniaturization of the detection step, we have studied the pitfalls of the electrochemical TMB detection. (kobv.de)

Flow cytometry -Immunomagnetic capture of exosomes on the surface of the Dynabeads allow for a clear and defined FFC/SSC detection typically in less than one hour. (thermofisher.com)

The tumor cell detection segment is further sub-segmented into optical methods and molecular methods. (dailybn.com)

In addition, methods based on antigen-antibody immunoreactivity also play an important role in ancient silk analysis. (springeropen.com)
The methods presented in this review proved to be fast, and effective and could be effective substitutes for chemical and physical methods, chemical and biochemical, producing results with greater accuracy, reliability and speed of analysis. (peertechzpublications.com)
For a long time, food analysis has always been based on physical, chemical, physicochemical, and biochemical methods, however, these are techniques that sometimes require a lot of time and have a low degree of precision. (peertechzpublications.com)
There are a few methods, though, which can be used for intact exosome analysis. (thermofisher.com)
Protein analysis -Specific monoclonal antibodies ( CD9 , CD63 , and CD81 ) allow for detection of cellular and exosomal antigens by Western blot analysis . (thermofisher.com)

Application of LMS integrated with qPCR enabled detection of a single oocyst/ml in dirty water sample concentrate. (usda.gov)

This situation has created an alarming public health emergency of international concern requiring the detection of this life-threatening viral candidate due to increased cases of newborn microcephaly associated with ZIKV infection. (frontiersin.org)

Molecular techniques have facilitated the identification, genotyping, and monitoring of food contamination sources, identification of genetically modified organisms, and use as food detection methods to avoid falsification through genetic markers. (peertechzpublications.com)

We focused on validating this method in a model system, since this is a necessary step before starting to analyze patient samples. (iiarjournals.org)
If you are looking to compare multiple protein samples on the same gel and want to keep exosomal protein complexes intact, you should check out the fast and gentle Dynabeads-based immunoprecipitation method using Invitrogen Protein A or Protein G in combination with your primary antibody of choice. (thermofisher.com)

Anatomy6

Organisms7

Diseases4

Chemicals and Drugs20

Analytical, Diagnostic and Therapeutic Techniques and Equipment26

Phenomena and Processes5

Disciplines and Occupations4

Technology, Industry, Agriculture5

Information Science2

Health Care7

Separation14

Enrichment11

Polymerase chain re2

Assay for the detection2

CTCs17

Isolation5

Pathogen6

Immunological2

Assays3

Immunoassay3

Bead4

Sensitivity7

Beads1

Pathogens5

Multiplex2

Filtration1

Nanoparticles2

Salmonella1

Quantification2

Oocysts3

Prognostic1

Target bacteria1

Foodborne1

Epithelial1

Cryptosporidium1

Clinical5

Rapid4

Bacteria1

Analytical methods3

Bacterial1

Nucleic1

Widely1

DTCs1

Pathogenic1

Approaches1

Reagents2

Diagnosis1

Reproducible1

Cerebrospinal fluid1

Bone1

Concentration1

Approach3

Capture1

Molecular methods1

Analysis5

QPCR1

Viral1

Markers1

Samples2