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  • incubation
  • The results of spectral analysis indicating HmuY-haem complex formation were corroborated by non-reducing SDS-PAGE analysis of samples obtained at different time intervals during incubation of HmuY with immobilized methaemoglobin. (nih.gov)
  • haemoglobin
  • HmuY was also capable of scavenging haem from oxyhaemoglobin pre-treated with the K-gingipain (Kgp).This is the first demonstration of a haemophore working in conjunction with proteases to acquire haem from haemoglobin.In addition, HmuY was able to extract haem from methaemalbumin, and could bind haem, either free in solution or from methaemoglobin, even in the presence of serum albumin. (nih.gov)
  • patients
  • The Prevalence of Methemoglobin in HIV Infected Patients on Chronic Dapsone Therapy for Prophylaxis of Pneumocystis jirovecii Infection. (masimo.ca)
  • activity
  • Haem pickup by HmuY from methaemoglobin-agarose.HmuY (16 µM) was incubated with methaemoglobin-agarose beads and after centrifugation the agarose-free supernatant was sampled and analyzed by non-reducing SDS-PAGE, and stained with TMB/H2O2 to reveal haem-associated peroxidise activity before being stained with Coomassie brilliant blue (CBB). (nih.gov)
  • production
  • 6. The explanation of this phenomenon of methemoglobin production is not only of importance so far as this special reaction is concerned, but also because it suggests an explanation for the manner in which pathological effects are produced by those bacteria which apparently produce no soluble toxin. (rupress.org)
  • result
  • The pKi values and the rate constants of methemoglobin S are strikingly different from those of methemoglobin A. This result is explained in terms of different electrostatic contributions to the free energy of heme linkage arising from differences in the environments of ionizable groups at the surfaces of the two molecules. (edu.ng)
  • solution
  • In addition, HmuY was able to extract haem from methaemalbumin, and could bind haem, either free in solution or from methaemoglobin, even in the presence of serum albumin. (nih.gov)