Metabolites isotopically label. Medical search. Frequent questions

However, for quantification of the drugs applying HF-LPME, further development is required to incorporate the use of isotopically labelled analogues. (gla.ac.uk)

Isotopically labeled internal standards are used for precise and accurate quantification. (cdc.gov)
A sensitive LC-MS/MS method was developed and validated for the simultaneous quantification of MPV and its metabolite ß-d-N4-hydroxycytidine (NHC) in human plasma and saliva. (lstmed.ac.uk)
However, for multi-analytes quantification, the number of available isotopically labelled internal standards is limited. (palsystem.com)
Lipid research encompasses the large-scale profiling of cellular lipids, the quantification of specific lipids in biological samples and the documentation of the interactions between lipids and other lipids, proteins and metabolites within the body. (sanbio.nl)
Though alterations in the NAD + to nicotinamide ratio and the NAD + to NADH ratio are anticipated by models to account for the effects of calorie restriction, the nature of a putative change in NAD + metabolism requires analytical definition and quantification of the key metabolites. (biomedcentral.com)
The metabolomic division of our facility allows small-molecule quantification, with a special focus on drugs and their metabolites. (edu.pl)
Our facility also offers several proteomic relative quantification strategies, including label-free analysis and stable isotope labeling, both in vivo and in vitro, using a variety of labeling tags. (edu.pl)
The upgrade to MassChrom® 25-OH-vitamin D3/D2 analysis allows for the separation of the main metabolites of vitamins D3 and D2, 25-Hydroxycholecalciferol and 25-Hydroxyergocalciferol, from their epimeric forms 3-epi-25-OH-vitamin D3 and 3-epi-25-OH-vitamin D2, hence allowing for separate quantification by LC MS/MS. (zskck.com)

For instance, isotope-assisted metabolomics technology has enabled the mapping of a global metabolic network, estimation of flux at branch points of metabolic pathways, and assignment of elemental formulas to unknown metabolites. (mdpi.com)
Solution conditions were tested at different pH values (from 7.2-5.0) and two temperatures (25 °C and 55 °C) to form a greater database of known chemical shifts that, in future work, will be used against unknown metabolites. (boisestate.edu)

Two of the urinary metabolites measured are unique to specific pyrethroids: 4-fluoro-3-phenoxyacetic acid from cyfluthrin and cis-3-(2,2-dibromovinyl)-2,2-dimethylcyclopropane carboxylic acid from deltamethrin. (cdc.gov)
The other urinary metabolites can result from exposure to any of several pyrethroid insecticides. (cdc.gov)
The specific organophosphorus insecticides with urinary metabolites measured are chlorpyrifos and chlorpyrifos-methyl (3,5,6-trichloro-2-pyridinol or TCPy), diazinon (2,-isopropyl-4-methyl-6-hydroxypyrimidine or IMPY), malathion (malathion dicarboxylic acid or MDA), and methyl and ethyl parathion (para-nitrophenol or PNP). (cdc.gov)
Methyl parathion has extremely limited agricultural use, and ethyl parathion is no longer registered for use in the U.S. Each of these measured urinary metabolites can also result from environmental degradation of the parent organophosphorus insecticide. (cdc.gov)
Detecting any of these urinary metabolites in a person's urine may indicate exposure to the specific organophosphorus insecticide or to the environmental breakdown product or both. (cdc.gov)
Of stanozolol urinary metabolites in humans by gas chromatography-mass spectrometry. (ligadebolsa.com)

Because pyrethroid insecticides are widely used in the U.S. population, measuring urinary pyrethroid metabolites is important to evaluate human exposure and potential for health effects. (cdc.gov)

NAIL-MS (short for nucleic acid isotope labeling coupled mass spectrometry) is a technique based on mass spectrometry used for the investigation of nucleic acids and its modifications. (wikipedia.org)
Therefore, cells in culture are first fed with stable isotope labeled nutrients and the cells incorporate these into their biomolecules. (wikipedia.org)
Unlabeled nucleosides can therefore be distinguished from their stable isotope labeled isotopologues. (wikipedia.org)
In general, cells are cultivated in unlabeled or stable (non-radioactive) isotope labeled media. (wikipedia.org)
For E.coli (bacteria) the minimum medium M9 can be used and supplemented with the stable isotope labeled variants of the needed salts. (wikipedia.org)
In S.cerevisiae (yeast) there are currently two possibilities: First, the use of commercially available complete growth medium, which enables labeling with 13C-carbon and/or 15N-nitrogen and second the use of minimal YNB medium which has to be supplemented with several amino acids and glucose which can be added as stable isotope labeled variants in order to achieve 13C-carbon, 15N-nitrogen and 2H-hydrogen labeling of RNA. (wikipedia.org)
While labeling in model organisms like E.coli and S.cerevisiae is fairly simple, stable isotope labeling in cell culture is much more challenging as the composition of the growth media is much more complex. (wikipedia.org)
Neither the supplementation of stable isotope labeled glucose nor the supplementation of stable isotope labeled variants of simple precursors of nucleoside biosynthesis such as glutamine and/or aspartate is sufficient for a defined mass increase higher than 2 Da. (wikipedia.org)
Instead, most cells kept in cell culture can be fed with stable isotope labeled methionine for labeling of methyl groups and with stable isotope labeled variants of adenin and uridine for labeling of the nucleoside's base body. (wikipedia.org)
NAIL-MS can be used to produce stable isotope labeled internal standards (ISTD). (wikipedia.org)
Isotope-labeling is a useful technique for understanding cellular metabolism. (mdpi.com)
In the present study, diverse cell biological and analytical tools such as laser ablation inductively-coupled plasma mass spectrometry (LA-ICP-MS), isotope labeling, and nano-scale secondary ion mass spectrometry (NanoSIMS) were employed to better understand the distribution and activation process of KP2156 (in comparison to free oxaliplatin and a non-albumin-binding succinimide analogue). (microbial-ecology.de)
b: Further detailed isotope labeling is data processing costs only. (uky.edu)

After culture stabilization, [U-13C] glucose was added as an isotopically labeled metabolic precursor. (tno.nl)
Using computer modeling of the 12C contents and 13C labeling kinetics, the metabolic fluxes in the gut microbial pathways for synthesis of lactate, formate, acetate, and butyrate were determined separately for glucose and unlabeled background substrates. (tno.nl)
Metabolites are small molecular compounds that are found in various stages of the metabolic processes within an organism. (boisestate.edu)
Molecular compounds such as glucose and sodium pyruvate can be isotopically labeled with C-13 to trace metabolic flux within a living cell using nuclear magnetic resonance (NMR) spectroscopy. (boisestate.edu)
Fifteen metabolites were found to be significantly dysregulated in CAP patients, which were mainly mapped to the metabolic pathways of sphingolipid, arginine, pyruvate and inositol phosphate. (biomedcentral.com)
This study demonstrates that serum metabolomics approaches based on the LC-MS/MS platform can be applied as a tool to reveal metabolic changes during CAP and establish a metabolite signature related to disease severity. (biomedcentral.com)
The metabolic fate of isotopically labeled trimethylamine- N -oxide (TMAO) in humans. (claracho.com)

Human metabolism of pyrethroids occurs rapidly, and metabolites are eliminated in urine and bile over several days. (cdc.gov)
Human metabolism of these organophosphorus insecticides results in the specific metabolites named above, dialkylphosphate metabolites, and other metabolites, all which are eliminated in urine over a few days. (cdc.gov)
Major differences in the pharmacokinetics between CPF and metabolite doses were observed within the first 3 h of exposure, due to the required metabolism of CPF to initially form TCPy and DETP. (cdc.gov)

This method can be used to assess human exposure to select non-persistent pesticides by measuring their metabolites in urine. (cdc.gov)
High-performance liquid chromatography (HPLC) has been used to measure isotopically labelled pyridine and its metabolites in urine (Shaker et al. (cdc.gov)
The results of this comparison showed that both methods were acceptable for the detection of exposures to fentanyl analogs and metabolites in urine. (cdc.gov)
Urine was selected as biological matrix, and the group of chosen analytes were 14 anti-hypertensive drugs and their metabolites with very different physical-chemical properties. (gla.ac.uk)

Indeed, evidence obtained with isotopically labeled 20-carbon fatty acids incubated with sheep vesicular glands, an abundant source of PGs, confirmed this assumption. (jci.org)

The CSC can identify putative features and confirm by preparing noncommercially available authentic standards (and labeled analogs). (cancer.gov)
To achieve this sensitivity, two acceptable methods for the detection of human exposure to seven fentanyl analogs and three metabolites were developed using either off-line 96-well plate SPE or on-line SPE. (cdc.gov)

it suggests that environmental exposure to low dose mixtures of pesticides and metabolites will not impact their pharmacokinetics. (cdc.gov)
Using a combination of LC-MS and GC-MS it is common for laboratories to analyse 400-500 pesticides and metabolites, ideally each at the 10 ng/g concentration in a single extract. (thermofisher.com)

For example, the medium can contain glucose labeled with six carbon-13 atoms (13C) instead of the normal carbon-12 (12C). (wikipedia.org)

The plant genus Cannabis is a prolific producer of unique pharmaceutically relevant metabolites, commonly referred to as cannabinoids. (researchgate.net)

Comparative pharmacokinetics of chlorpyrifos versus its major metabolites following oral administration in the rat. (cdc.gov)

The use of atmospheric pressure chemical ionisation (APCI) and an isotopically labelled internal standard assures precision and robustness and minimises ion suppression effects. (zskck.com)

Analysis was performed using stable isotopically labelled (SIL) internal standards (IS). (lstmed.ac.uk)

The QuPPe extracts often contain high amounts of co-extractives which can impact on the robustness of the method, thus isotopically labelled standards are recommended to improve the precision of analysis. (thermofisher.com)
aromaLAB GmbH is proud to announce that aromaLAB's synthesis department achieved a successful certification of DIN EN ISO 9001 for the development and production of unlabelled and isotopically labelled standards. (labocoranalitica.es)

Metabolite concentrations and isotopic labeling were determined by NMR, GC, and enzymatic methods. (tno.nl)

Choline and one-carbon metabolite response to egg, beef and fish among healthy young men: a short-term randomized clinical study. (claracho.com)

The alternation trends of five metabolites among the three groups including sphinganine, p-Cresol sulfate, dehydroepiandrosterone sulfate (DHEA-S), lactate and l -arginine in the validation cohort were consistent with those in the discovery cohort. (biomedcentral.com)
Combining lactate, sphinganine and DHEA-S as a metabolite panel for discriminating severe CAP from non-severe CAP exhibited a better AUC of 0.911 (95% confidence interval 0.825-0.998) than CURB-65, PSI and APACHE II scores. (biomedcentral.com)

CSC synthesizes custom fluorescent and light-activated probes, including fluorophore-labeled peptides, fluorescent or photoactivated drugs, fluorescent versions of native biological small molecules, and other literature-reported fluorescent tools, for biological research. (cancer.gov)
Posttranslational linkage of a thiol to the alpha-carbon of an amino acid residue is unprecedented in ribosomally synthesized peptides or proteins, and very rare in secondary metabolites. (rcsb.org)

And, there is no single workflow which can provide large-scale metabolomics coverage in particular for polar metabolites. (palsystem.com)

article{osti_1500021, title = {Augmenting the Calvin-Benson-Bassham cycle by a synthetic malyl-CoA-glycerate carbon fixation pathway}, author = {Yu, Hong and Li, Xiaoqian and Duchoud, Fabienne and Chuang, Derrick S. and Liao, James C.}, abstractNote = {The Calvin-Benson-Bassham (CBB) cycle is presumably evolved for optimal synthesis of C3 sugars, but not for the production of C2 metabolite acetyl-CoA. (osti.gov)

Institut für Pharmazie und Molekulare Biotechnologie, Limonoids are typical secondary metabolites of Citrus species. (gotomydoctor.com)

or 2H-labeled CPF, diethyl-D10 (deuterium labeled) on the side chain) were exploited to compare the pharmacokinetics of CPF with TCPy, and DETP. (cdc.gov)

The amino acid serine, which is a downstream metabolite generated from betaine via the homocysteine remethylation pathway is another necessary step. (medscape.com)

This pathway converts a C3 metabolite to two acetyl-CoA by fixation of one additional CO2 equivalent, or assimilates glyoxylate, a photorespiration intermediate, to produce acetyl-CoA without net carbon loss. (osti.gov)
According to the LIPID Metabolite and Pathway Strategy (LIPID MAPS) project, lipids can be categorised into eight major groups: fatty acyls, glycerophospholipids, glycerolipids, sterol lipids, sphingolipids, polyketides, prenol lipids and saccharolpidpids. (sanbio.nl)

For metabolite profiling chemical derivatization has been used to improve MS sensitivity and LC retention. (palsystem.com)
1-D NMR spectra can then be collected to observe specific chemical shifts of known C-13 labeled metabolites in living cells. (boisestate.edu)
This NMR spectra can be accurately replicated as each chemical shift is derived from its corresponding metabolite. (boisestate.edu)

The Calvin-Benson-Bassham (CBB) cycle is presumably evolved for optimal synthesis of C3 sugars, but not for the production of C2 metabolite acetyl-CoA. (osti.gov)

Metabolomic and pharmacokinetic studies are performed by the Laboratory of Quantitative Analysis of Drugs and Metabolites (registration no. 3089, National Chamber of Laboratory Diagnosticians), which is part of the Mass Spectrometry Facility. (edu.pl)

How the labeling of RNA molecules is achieved depends on the model organism. (wikipedia.org)

However, biomonitoring of TCPy, DEP and DETP may be reflective of an individual's contact with both the parent pesticide and exposure to these metabolites in the environment. (cdc.gov)
METHODS: We evaluated the urinary acrylonitrile metabolite, 2CyEMA, as a biomarker of exposure to cigarette smoke in the U.S. population-representative data from the National Health and Nutritional Examination Survey (NHANES). (cdc.gov)

On top of this, lipids are changing constantly in response to changes in their environment and interactions with metabolites. (sanbio.nl)
Lipids make up a large proportion of all metabolites, making them important potential biomarkers. (sanbio.nl)

Selected differential metabolites between CAP patients versus controls, and between the severe CAP group versus non-severe CAP group, were confirmed by targeted mass spectrometry assays in a validation cohort ( n = 73, including 32 non-severe CAP, 19 severe CAP and 22 controls). (biomedcentral.com)
Mesterolone and its metabolites have been isolated by liquid-liquid extraction and analyzed by gas chromatography-mass spectrometry (gc-ms) after acylation or. (ligadebolsa.com)

Thereafter, all nucleotides are 5 Da heavier than their unlabeled isotopologues due to a complete carbon labeling of the ribose. (wikipedia.org)
This enables labeling with 13C-carbon, 15N-nitrogen, 34S-sulfur and 2H-hydrogen. (wikipedia.org)

Clinical pharmacokinetic studies are underway utilising this method for determination of MPV and its metabolite in patients with COVID-19 infection. (lstmed.ac.uk)
Pearson's correlation analysis was performed to assess relationships between the identified metabolites and clinical severity of CAP. (biomedcentral.com)

For most NAIL-MS approaches it is crucial that the labeled nucleosides are more than 2 Da heavier than the unlabeled ones. (wikipedia.org)

NAIL-MS can be used to investigate RNA modification dynamics by changing the labeled nutrients of the corresponding growth medium during the experiment. (wikipedia.org)

NDAAs were analysed using sequential tandem MS. The CID products parallel the metabolites produced in biological systems. (uoguelph.ca)

Metabolomic feature identification - The presence and amount of a specific metabolite in cells, biofluids, tissues, or organisms can be directly reflective of biochemical processes occurring in a biological system in response to its environment. (cancer.gov)
The aim of this specific research is to compile an NMR database of known C-13 labeled metabolites in varying solution conditions. (boisestate.edu)

The study objective was to quantitatively evaluate the pharmacokinetics of the individual metabolites relative to their formation following a dose of CPF. (cdc.gov)

Urinary excretion rates for orally administered TCPy and DETP relative to 13C-CPF or 2H-CPF derived 13C-TCPy and 2H-DETP were consistent with blood pharmacokinetics, and the urinary clearance of metabolite dosed groups were comparable with the results for the 13C- and 2H-CPF groups. (cdc.gov)

Special care must be taken when supplementing the medium with FBS (fetal bovine serum), as it also contains small metabolites used for the biosynthesis of nucleosides. (wikipedia.org)

In biological systems in which pyridine may have been metabolized or may itself be a metabolite, consideration should be given to the possible binding of the analyte by endogenous substances in the biological system. (cdc.gov)
The "minimum performance limits" (MRPL) for the prohibited antibiotic substances chloramphenicol (MRPL = 0.3 ppb) and nitrofuran metabolites (MRPL = 1.0 ppb) in animal foods such as honey, which have been in force in the EU for many years, have been replaced by "reference points for action" (RPA) and tightened significantly. (labocoranalitica.es)

After cultivation and appropriate labeling of the cells, they are generally harvested using phenol/chloroform/guanidinium isothiocyanate. (wikipedia.org)

This study identified 16 sugar alcohols and related metabolites in the blood of these patients that were associated with an increased risk of heart attack and stroke. (chaneyhealth.com)

In simple terms this means that high-tech equipment and computing were used to measure hundreds of metabolites in the blood of the patients and, in this case, correlate each of them with the occurrence of heart attacks and strokes over the next three years. (chaneyhealth.com)

3 h post-dosing) pharmacokinetics for both treatment groups and metabolites were very comparable. (cdc.gov)

The CSC routinely prepares isotopically labeled metabolites for the use in these studies. (cancer.gov)
The complete primary and three-dimensional solution structures of subtilosin A (1), a bacteriocin from Bacillus subtilis, were determined by multidimensional NMR studies on peptide produced using isotopically labeled [(13)C,(15)N]medium derived from Anabaena sp. (rcsb.org)

Anatomy13

Organisms16

Chemicals and Drugs139

Analytical, Diagnostic and Therapeutic Techniques and Equipment33

Phenomena and Processes46

Disciplines and Occupations3

Technology, Industry, Agriculture3

Information Science3

Health Care7

Analogues1

Quantification8

Unknown metabolites2

Urinary metabolites6

Pyrethroid metabolites1

Isotope13

Metabolic7

Metabolism3

Urine4

Fatty acids1

Analogs2

Pesticides2

Glucose1

Cannabinoids1

Chlorpyrifos1

Ionisation1

Stable1

Standards2

Concentrations1

Choline1

Lactate2

Peptides2

Metabolomics1

Synthetic1

Secondary1

Deuterium1

Downstream1

Pathway2

Chemical3

Sugars1

Drugs1

Molecules1

Exposure2

Lipids2

Mass2

Carbon2

Clinical2

Approaches1

Growth1

Tandem1

Specific2

Evaluate1

Results1

Small1

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Cells1

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Studies2